JP2018052990A - がん治療のためのegfr−sglt1相互作用の標的化 - Google Patents
がん治療のためのegfr−sglt1相互作用の標的化 Download PDFInfo
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Abstract
Description
本出願は、2013年5月8日に出願された米国仮出願第61/821,028号に基づく優先権を主張し、この出願は、その全体が参照によって本明細書に組み込まれる。
政府支援
アメリカがん協会(RSG−09−206−01)
米国国防総省前立腺癌研究プログラム(W91ZSQ8334N607)
cancer、BMC Cancer7巻:142頁(2007年))。
したがって、EGFRチロシナーゼインヒビターに抵抗性であるがん細胞を適切に処置することができる方法および組成物が当該技術分野において必要とされる。
本明細書は、例えば、以下の項目も提供する。
(項目1)
上皮増殖因子受容体(EGFR)とナトリウム/グルコース共輸送体1(SGLT1)の間の結合相互作用を不安定化させる化合物を含む組成物。
(項目2)
前記化合物が、ペプチドおよびペプチド類似体のうちの少なくとも1つである、項目1に記載の組成物。
(項目3)
前記ペプチドが、EGFRのSGLT1相互作用ドメインに由来する、項目2に記載の組成物。
(項目4)
前記ペプチドが、アミノ酸配列LVWKQSCSSTSSTH(配列番号001)を含む、項目2に記載の組成物。
(項目5)
前記ペプチドが、配列番号002、配列番号003、配列番号004、配列番号005、配列番号006、配列番号007、配列番号008、配列番号009、配列番号0010および配列番号0011のうちの少なくとも1つに実質的に類似する化合物を含む、項目2に記載の組成物。
(項目6)
前記化合物が、フロリジンSGLT1インヒビターおよびフロリジン様SGLT1インヒビターのうちの少なくとも1つである、項目1に記載の組成物。
(項目7)
対象におけるがん細胞を処置する方法であって、該対象に、上皮増殖因子受容体(EGFR)とナトリウム/グルコース共輸送体1(SGLT1)の間の結合相互作用を不安定化させる化合物を投与することを含む方法。
(項目8)
前記化合物が、ペプチドおよびペプチド類似体のうちの少なくとも1つである、項目7に記載の方法。
(項目9)
前記ペプチドが、アミノ酸配列LVWKQSCSSTSSTH(配列番号001)を含む、項目8に記載の方法。
(項目10)
前記ペプチドが、配列番号002、配列番号003、配列番号004、配列番号005、配列番号006、配列番号007、配列番号008、配列番号009、配列番号0010および配列番号0011のうちの少なくとも1つを含む、項目8に記載の方法。
(項目11)
前記対象にチロシンキナーゼインヒビターを投与することをさらに含む、項目7に記載の方法。
(項目12)
前記対象にプロテアソーム機構を標的とする化合物を投与することをさらに含む、項目7に記載の方法。
(項目13)
前記がん細胞が、乳がん細胞、前立腺がん細胞および結腸がん細胞のうちの少なくとも1つである、項目7に記載の方法。
(項目14)
前記ペプチドを使用してEGFR−SGLT1相互作用によって制御される下流の生存経路を同定することにより、がん治療の標的を同定することをさらに含む、項目8に記載の方法。
(項目15)
対象におけるがん細胞を処置する方法であって、該対象に、チロシンキナーゼインヒビターと、フロリジンSGLT1インヒビターおよびフロリジン様SGLT1インヒビターのうちの少なくとも1つとを投与することを含む方法。
(項目16)
前記投与が、上皮増殖因子受容体(EGFR)とナトリウム/グルコース共輸送体1(SGLT1)の間の結合相互作用を不安定化させる、項目15に記載の方法。
(項目17)
前記対象にチロシンキナーゼインヒビターを投与することをさらに含む、項目15に記載の方法。
(項目18)
前記がん細胞が前立腺がん細胞である、項目17に記載の方法。
(項目19)
前記チロシンキナーゼインヒビターが、ゲフィチニブ、エルロチニブ、イコチニブ、ムブリチニブ、バンデタニブ、ラパチニブ、ペリチニブ、カネルチニブ、ネラチニブ、アファチニブおよびダコミチニブのうちの少なくとも1つである、項目17に記載の方法。
(項目20)
前記フロリジンSGLT1インヒビターが、カナグリフロジンおよびダパグリフロジンのうちの少なくとも1つである、項目15に記載の方法。
・SGLT1とのその十分な相互作用およびSGLT1との相互安定化に必要とされるEGFRの重要なマイクロドメイン;
・EGFR−SGLT1相互作用におけるEGFRの活性化/不活性化の効果;
・前立腺がんの組織および細胞株におけるEGFRおよびSGLT1の発現測定;
・EGFRチロシンインヒビターに対する前立腺がん細胞の感受性に対するSGLT1の阻害効果;
・合成されたペプチドであるESD−01のアミノ酸配列;
・がん細胞におけるEGFRおよびSGLT1タンパク質の安定性に対するESD−01の効果;および
・非がん細胞(HEK293)およびインビトロで培養された数種類のがん細胞の生存に対するESD−01の効果。
・L−アミノ酸またはD−アミノ酸のいずれかで作製されたESD−01ペプチドは、インビトロにおけるがん細胞の死滅に同等に有効である。
kinase activity、Cancer Cell13巻(5号):385〜393頁(2008年))。ある実施形態において、EGFR−SGLT1相互作用は、がんについてのEGFRに基づく治療を改善するための重要な標的であり得る。
Clinical Oncology、23巻(3号):455〜460頁(2005年);Gross Mら、A phase II trial of docetaxel and erlotinib as first−line therapy for elderly patients with androgen−independent prostate cancer、BMC Cancer7巻:142頁(2007年))。ある実施形態において、SGLT1およびEGFRは、前立腺がんの増殖を相乗的に促進することができる。一実施形態において、SGLT1の阻害が、EGFRインヒビターに対して前立腺がん細胞を感作させることができるかどうかを試験するために、前立腺がん細胞株(例えば、PC3およびLNCaP(ともにEGFRとSGLT1に対して陽性))をSGLT1インヒビター(例えば、フロリジンおよびフロリジン誘導体、例えばカナグリフロジンおよびダパグリフロジン)の存在/非存在下でEGFRチロシンキナーゼインヒビター(例えば、ゲフィチニブ、エルロチニブ、イコチニブ、ムブリチニブ、バンデタニブ(Vandertanib)、ラパチニブ、ペリチニブ、カネルチニブ、ネラチニブ、アファチニブおよびダコミチニブ)を用いて処理され得る(Ehrenkranz JRら、Phlorizin:a review、Diabetes/Metabolism Research and Reviews21巻(1号):31〜38頁(2005年))。一実施形態において、処置の増殖阻害効果を決定することができる。例えば、ある実施形態において、MTTアッセイに供する前に、EGFRインヒビター(ゲフィチニブ、10μM;エルロチニブ、10μM)の有無でSGLT1インヒビターであるフロリジン(50μM)を用いて、48時間細胞を処理することができる。対照細胞のOD値を人為的に1に設定することができる。全ての実験は、少なくとも3回繰り返されてもよい。アスタリスク記号は、連結された群間の統計的有意性を示す。
Ther.4巻(9号):306〜312頁(2012年))。
1 Expression Increases in Human Diseased Prostate、J. Cancer Sci. Ther.、4巻(9号):306〜312頁(2012年))。PBSで3回洗浄後、組織を、10%ロバ血清を含有するPBSに溶解させた、Alexa Fluor488コンジュゲート化ロバ抗ウサギIgGとAlexa Fluor 594コンジュゲート化ロバ抗マウスIgGの混合物とともに室温で30分間インキュベートした。次に、染色した試料を室温にてPBSで3回(洗浄1回あたり5分間)洗浄した。蛍光画像を共焦点顕微鏡(Olympus)で撮像し、分析した。細胞核を4’,6−ジアミジノ−2−フェニルインドール(DAPI)で染色した。
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