JP2018050510A - Powder containing phospholipid derived from milk and method for producing the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a method for improving a yield of phospholipid by inhibiting an aggregate of protein from remaining in a powder containing phospholipid derived from milk produced using a milk product as a starting material.SOLUTION: The powder containing phospholipid derived from milk contains 45 to 70 wt% of lipid in a dry matter, has a content of phospholipid derived from milk contained in lipid of 20 wt% or more in a dry matter and has a median diameter of insoluble components of 10 μm or less when prepared as a 5% aqueous solution. The method for producing the powder containing phospholipid derived from milk comprises the steps of: decomposing a protein by adding a proteolytic enzyme to a starting material including a milk product; deactivating the proteolytic enzyme after decomposing the protein; separating the protein decomposed by membrane treatment after inactivating the proteolytic enzyme to produce a concentrated liquid; and drying the concentrated liquid.SELECTED DRAWING: None

Description

  The present invention relates to milk-derived phospholipid-containing powder and a method for producing the same.

Soy lecithin and egg yolk lecithin are widely used as a natural product-derived phospholipid crude product as an emulsifier in food production. In recent years, milk-derived phospholipids such as phosphatidylserine, phosphatidylcholine, and sphingomyelin have various physiological properties. It has been reported and attracted attention.
It has been reported that phosphatidylserine and phosphatidylcholine are deeply involved in the development and maintenance of nerve function and motor function, and sphingomyelin has an intestinal maturation function in infants. Sphingomyelin is a substance having a structure in which phosphocholine is bound to a ceramide skeleton composed of sphingosine and a fatty acid, and is present in large amounts in the brain and nerve tissues. Sphingomyelin is only slightly contained in soybean phospholipid and egg yolk phospholipid, but is abundant in milk and accounts for about 30% of phospholipid in milk.
Therefore, milk-derived phospholipid-rich powders that contain milk-derived phospholipids at high concentrations, such as phosphatidylserine, phosphatidylcholine, and sphingomyelin, are widely used as functional foods, breast milk substitutes, or pharmaceutical raw materials. can do.

  As a powder containing milk-derived phospholipid, a powder produced using butaselam as a raw material is known (for example, Patent Document 1). In the method described in Patent Document 1, the reducing solution of Batase lam or Batase rum powder is adjusted to an acidity of pH 4.0 to 5.0, and the isoelectric point precipitation of the protein produced by adding calcium chloride is removed by centrifugation or the like. Then, after adjusting the pH to neutral, and drying the concentrated solution obtained by ultrafiltration or microfiltration of the supernatant, a milk-derived complex lipid-containing powder containing milk-derived phospholipids is obtained. ing.

  In addition, dairy products such as buttermilk powder are known to extract phospholipids in dairy products using chloroform / methanol / water (4/8/3) solvents or organic solvents such as acetone. (Patent Document 2). In this method, phospholipids can be concentrated to a purity of 90% or higher.

JP 2007-89535 A JP-A-3-47192

When the pH is adjusted and the protein contained in the starting material is agglomerated and precipitated, as in Patent Document 1, the centrifugation or the like is performed. Agglomeration occurs again while keeping the solution acidic. For this reason, protein aggregates remain in the obtained powder. When this powder is used as a food material, lipid contained in the powder becomes oil droplets and lactose and minerals dissolve in water, but the remaining protein aggregates become insoluble components. Since the median diameter of these insoluble components is as relatively large as 30 μm or more, when a food containing a food material is eaten, a “grainy” texture is felt.
In addition, when protein is aggregated and precipitated, about 30% of the phospholipid contained in the starting material is taken into the precipitate, and the phospholipid is also removed in the step of removing the protein aggregate precipitate. The yield collected as a phospholipid-containing powder will be low.
On the other hand, in the method using the organic solvent of Patent Document 2, since it is a method using an organic solvent that is not approved for the preparation of food materials, it is difficult to use the obtained milk-derived phospholipid-containing powder for food. is there.
The present invention is intended to solve these problems, and suppresses protein aggregates remaining in a milk-derived phospholipid-containing powder produced using a dairy product as a starting material, thereby improving the yield of phospholipids. It is an object to improve.

The present invention contains 45 to 70% by weight of lipid in the dried product, the content of the phospholipid derived from milk contained in the lipid in the dried product is 20% by weight or more, and is an insoluble component in a 5% aqueous solution. Is a milk-derived phospholipid-containing powder having a median diameter of 10 μm or less.
The present invention also includes a step of degrading protein by adding a proteolytic enzyme to an aqueous starting material solution containing dairy products, a step of deactivating the enzyme after degrading the protein, and after deactivating the enzyme, A method for producing milk-derived phospholipid-containing powder, comprising a step of separating a degraded protein by membrane treatment to obtain a concentrated solution, and a step of drying the concentrated solution.
Furthermore, the present invention includes a step of acidifying an aqueous solution of a starting material containing dairy products, a step of removing aggregates produced by adding calcium chloride by centrifugation or the like, and obtaining a supernatant; A method for producing a milk-derived phospholipid-containing powder, comprising a step of concentrating by treatment, a step of solubilizing a remaining aggregate by alkalizing the concentrate, and a step of drying the concentrate.

According to the present invention, since the median diameter of the insoluble component when the milk-derived phospholipid-containing powder is made into an aqueous solution is reduced, the “roughness” texture can be suppressed even when used as a food material.
Further, according to the production method of the present invention, the protein contained in the starting material is reduced in molecular weight by enzymatic degradation and then removed, so that protein aggregates are prevented from remaining in the powder. It can be prevented from being removed and the yield of phospholipids can be improved. According to the present invention, the recovery rate of the phospholipid in the raw material can be 90% or more, and the yield of the final product can be 15% or more of the solid content of the raw material.
Moreover, in the conventional manufacturing method, since the removed protein aggregate precipitate was generated as a semi-solid by-product, the treatment was separately required. On the other hand, since the membrane permeate generated as a by-product in the present production method is a liquid, it is easier to handle than the conventional semi-solid by-product. Further, this liquid contains protein degradation products such as peptides and amino acids obtained by degrading proteins in the raw material with enzymes, lactose and minerals, and can also be used as a milk solid source for foods and the like.
The milk-derived phospholipid-containing powder of the present invention, or the milk-derived phospholipid-containing powder and by-products obtained by the production method of the present invention can be used as functional foods, breast milk substitutes or pharmaceutical raw materials.

Hereinafter, a method for producing the milk-derived phospholipid-rich powder of the present invention will be described.
The content of phospholipid in butter lamb flour is 5% by weight or more, which is 10 times higher than 0.5% by weight in buttermilk powder. In addition, butaselam is inexpensive because it is produced as a by-product in the production of AMF (Anhydrous Milk Fat, butter oil). For this reason, Batase lamb and Batase lamb powder can be used as a raw material for milk-derived phospholipid-containing powder.
In the case of using butase lamb flour, the protein in protease is reduced in molecular weight by adding protease, which is a proteolytic enzyme, after dissolving in water and reducing it to a concentration of about 10% by weight.
Proteases used in the enzyme treatment include proteases derived from animals such as pepsin, chymotrypsin, trypsin and pancreatin, proteases derived from plants such as papain, bromelain and ficin, bacteria (lactic acid bacteria, Bacillus subtilis, actinomycetes), fungi (mushrooms, yeast, Any protease such as endoprotease and exoprotease produced by microorganisms such as mold) and algae can be used. The neutral / alkaline protease produced by the genus Bacillus and Aspergillus is particularly preferable because of its high protein resolution. The enzyme may be in any form regardless of liquid or powder such as purified enzyme, semi-purified enzyme, crude enzyme, and crushed cells. The solvent for preparing the enzyme solution is not particularly limited as long as the enzyme is not denatured or inactivated and does not cause any problem in food hygiene.
Specific examples of proteases that can be used include, for example, Sumiteam FP-G (manufactured by Shin Nippon Chemical Industry Co., Ltd.), Sumiteam LP-50D (manufactured by Shin Nippon Chemical Industry Co., Ltd.), Sumiteam MP (manufactured by Shin Nippon Chemical Industry Co., Ltd.), Protin SD -AY10 (Amano Enzyme), Protin NY100 (Amano Enzyme), Peptidase R (Amano Enzyme), Proteax (Amano Enzyme), Protease M `` Amano '' SD (Amano Enzyme), Protease P "Amano" 6SD (Amano Enzyme), Bromelain F (Amano Enzyme), Samoaze PC10F (Amano Enzyme), Pandidase NP-2 (Yakult Yakuhin Kogyo), Aroase NP-10 (Yakult Yakuhin Chemical) Kogyo Co., Ltd., Orientase 22BF (manufactured by HIBI), Orientase OP (manufactured by HIBI), Nucleicin (manufactured by HI), Multifect PR 6L (Du Pon), Multifect PR 7L (DuPont), Actinase AS (Kaken Pharma), Denateam AP (Nagase ChemteX), Maxipro PSP (DSM), Maxipro FPC (DSM), Entilon There are NBS-100 (manufactured by Toto Kasei), Alcalase (manufactured by Novozymes), Protamex (manufactured by Novozymes), and flavorzyme (manufactured by Novozymes).
Regarding the enzyme treatment conditions, the enzyme reaction temperature and pH do not necessarily have to be reacted at the optimum temperature and pH of the enzyme, and it may be preferable to carry out the reaction at a temperature and pH that can prevent flavor deterioration and spoilage. The reaction time is 5 minutes to 24 hours, but preferably within 5 hours from the viewpoint of microbial growth.
One enzyme may be added, but two or more enzymes may be used in combination.

The obtained enzyme-degraded batase lam contains proteins, peptides, amino acids, lactose, minerals, and lipid components including the target phospholipid. Membrane separation technology is applied to remove and reduce components other than phospholipids. Proteins and lipids contained in Bataselam cannot permeate the ultrafiltration membrane or microfiltration membrane. On the other hand, peptides, amino acids and other components such as lactose and minerals produced by reducing the molecular weight of proteins can permeate the ultrafiltration membrane or microfiltration membrane. For this reason, by concentrating and diafiltering the protease-treated bathase lam with an ultrafiltration membrane or a microfiltration membrane, components other than lipids can be removed to the permeate side and phospholipids can be concentrated. The proportion of the lipid component in the solid content of the resulting concentrate is as high as 45 to 70% by weight, but it can be easily pulverized with a spray drying apparatus normally used in the food industry, and the phospholipid content is 20%. % Of powder can be obtained.
The type of membrane used for membrane treatment is not limited to organic membranes and inorganic membranes. Polyvinylidene fluoride, polysulfone, polyethersulfone, polytetrafluoroethylene, polyethylene, polypropylene, polyimide, polyetherimide, polycarbonate, high molecular weight polyvinyl alcohol, etc. It may be made of an inorganic polymer such as ceramics, zeolite, zirconia, silica, or alumina. The shape of the separation membrane may be any shape such as a flat membrane, a hollow fiber membrane, a spiral membrane, and a ceramic membrane.
The separation membrane can be either an ultrafiltration membrane or a microfiltration membrane, or both, but removes peptides, amino acids, lactose and minerals in the enzymatic degradation batchase ram as much as possible and the lipid fraction as close to the concentrate as possible. In order to make it remain, it is desirable to use a microfiltration membrane, more preferably a microfiltration membrane having a fractional particle size of 0.1 to 1.4 μm.
As the drying means, for example, freeze drying, vacuum drying, hot air drying and the like can be used as appropriate.
The obtained powder of the present invention has a low protein, carbohydrate content and mineral content, contains 45 to 70% by weight of lipid in the dried product, and contains 20% by weight or more of phospholipid in the dried product. And a milk-derived phospholipid-containing powder having a median diameter of 10 μm or less as an insoluble component in a 5% aqueous solution.
It should be noted that the supernatant obtained by adjusting Batase lamb or Batase lamb powder reduced solution to an acidity of pH 4.0 to 5.0 and removing the isoelectric point precipitate of the protein produced by adding calcium chloride by centrifugation or the like The membrane is concentrated by ultrafiltration or microfiltration, and the resulting concentrate is adjusted to an alkaline pH of 9.0 to 10.0, solubilizing the protein aggregates and drying. It is possible to obtain a milk-derived phospholipid-containing powder similar to the above, but it is inferior to the above-mentioned method in terms of the recovery rate of phospholipid and the yield of the obtained powder.

  Next, an Example is given and this invention is demonstrated concretely.

Bataselam flour (TATUA) 20kg (of which 3.0kg of lipid, 1.6kg of phospholipid) is added to 180kg of water heated to 50 ° C, stirred and dissolved, and reduced to 10% solids by weight. Was prepared. Aspergillus oryzae-derived protease (Sumiteam LP-50D, Shinnippon Chemical Co., Ltd.) is added to this Bataselam reduced solution at 1% by weight of the protein weight of Bataselam flour, and reacted at 50 ° C. for 5 hours. Got. About the obtained enzyme-degraded butase ram solution, after inactivating the enzyme, a microfiltration membrane treatment with a fractional particle size of 1.4 μm was performed, the concentrated solution was recovered, and dried by freeze-drying treatment. Milk-derived phospholipid powder was obtained.
The weight of the obtained powder is 5.0 kg, of which the lipid is 2.9 kg and the phospholipid is 1.5 kg, the lipid in the dried powder of the obtained powder is 58 wt%, and the milk-derived phospholipid is 30 in the dried material. % By weight. In addition, the recovery rate of phospholipids in the powder obtained from the raw material butase lamb powder was 94%, the yield was 25%, and the median diameter of the insoluble component when the obtained powder was made into a 5% aqueous solution (accumulation on a volume basis). 50% particle diameter) was measured by a laser diffraction particle size distribution analyzer and found to be 5.1 μm.
The lipid and phospholipid content of the milk-derived phospholipid powder of the present invention were quantified by the following method.
After dissolving 1g of milk-derived phospholipid powder in 5mL of 1.25wt% potassium oxalate aqueous solution, add 6mL of ethanol, 15mL of diethyl ether and 15mL of petroleum ether sequentially and shake horizontally to extract the lipid into the solvent phase. The lipid content was calculated from the weight of oil remaining after volatilization of the solvent after recovery. Add 0.5 mL of perchloric acid and 2.0 mL of nitric acid to 25 mg of the above oil, heat at 100 ° C for 60 minutes, add 3 mL of nitric acid, and increase in temperature from 120 ° C to 140 ° C in 10 ° C increments for 10 minutes, 150 ° C to 170 ° C. Until then, each was heated at 10 ° C for 30 minutes to make wet ash, and then inorganic phosphorus was quantified using Phospha C-Test Wako. The obtained amount of inorganic phosphorus was multiplied by a phosphatidylserine conversion factor of 25.4 to convert it to a phospholipid amount.

[Comparative Example 1]
Batatarum reducing solution with a solid content of 10% by weight by adding 180 kg of water heated to 50 ° C to 20 kg of raw material butase lamb powder (TATUA) (of which 3.0 kg of lipid and 1.6 kg of phospholipid) Was prepared. The pH was adjusted to 4.4 by adding 10% hydrochloric acid to the Bataselam reducing solution. At the same time, calcium chloride was added to 0.02% by weight of the total amount, and then the casein was aggregated by maintaining at 50 ° C. for 30 minutes. The produced casein precipitate was completely removed by treating with a centrifuge to obtain a supernatant. The supernatant was recovered and powder was obtained by freeze-drying.
The weight of the obtained powder is 2.4 kg, of which the lipid is 1.4 kg, the phospholipid is 0.7 kg, the lipid in the dried product is 58% by weight, and the milk-derived phospholipid is 29 in the dried product. % By weight. Moreover, the recovery rate of phospholipids in the powder obtained from the raw material butase lamb powder was 44%, and the yield was 12%. Compared with Example 1, the recovery rate and yield of phospholipids were poor.
When the median diameter of the insoluble component (volume-based cumulative 50% particle diameter) was measured with a laser diffraction particle size distribution meter when the obtained powder was made into a 5% aqueous solution, it was 32.6 μm, which was larger than Example 1. .
[Comparative Example 2]
Bataselam flour (TATUA) 20kg (of which 3.0kg of lipid, 1.6kg of phospholipid) is added to 180kg of water heated to 50 ° C, stirred and dissolved, and reduced to 10% solids by weight. Was prepared. The obtained Batase lamb powder reduced solution was subjected to a microfiltration membrane treatment with a fractional particle size of 1.4 μm, and the concentrated solution was collected and dried by freeze-drying to obtain milk-derived phospholipid powder.
Of the 8.5 kg powder obtained, the lipid was 2.9 kg, the phospholipid was 1.5 kg, and the protein was 3.9 kg. The obtained powder was 34% by weight of the lipid and the milk-derived phospholipid was the dried product. The content was 18% by weight, which was lower than that in Example 1. This is probably because the protein in the raw material was not sufficiently removed to the permeate side, and the lipid and phospholipid contents were relatively low. The recovery rate of phospholipids in the powder obtained from the raw material butase lamb powder was 94%, and the yield was 43%.
When the obtained powder was made into a 5% aqueous solution, the median diameter of the insoluble component (volume-based cumulative 50% particle diameter) was measured by a laser diffraction particle size distribution analyzer and found to be 0.44 μm.
[Comparative Example 3]
Bataselam powder (TATUA) 20 kg (of which 3.0 kg of lipid, 1.6 g of phospholipid) is added with 180 kg of water heated to 50 ° C. and stirred and dissolved to obtain a 10% by weight solid content of Bataselam reducing solution. Was prepared. Aspergillus oryzae-derived protease (Sumiteam LP-50D, Shinnippon Chemical Co., Ltd.) is added to this Bataselam reduced solution at 1% by weight of the protein weight of Bataselam flour and reacted at 50 ° C. for 1 hour, followed by enzymatic degradation Bataselam solution Got. About the obtained enzyme-degraded butase ram solution, after inactivating the enzyme, a microfiltration membrane treatment with a fractional particle size of 1.4 μm was performed, the concentrated solution was recovered, and dried by freeze-drying treatment. Milk-derived phospholipid powder was obtained.
Of the obtained powder 7.8 kg, the lipid is 2.9 kg, the phospholipid is 1.5 kg, the protein is 3.2 kg, the lipid in the dried product is 37% by weight, the milk-derived phospholipid is the dried product Among them, 19% by weight was lower than that in Example 1. This is thought to be because the protein in the raw material was not sufficiently decomposed, so that the removal to the permeate side was insufficient, and the lipid and phospholipid contents were relatively low. The recovery rate of phospholipids in the powder obtained from the raw material butase lamb powder was 94%, and the yield was 39%.
The median diameter (volume-based cumulative 50% particle diameter) of the insoluble component when the obtained powder was made into a 5% aqueous solution was measured by a laser diffraction particle size distribution analyzer and found to be 0.42 μm.

By adding 450kg of water heated to 50 ° C to 50kg of raw material butase ram (Uelzena) (0.65kg of lipid, 0.33kg of solid 5kg solids) A reducing solution was prepared. Aspergillus oryzae-derived protease (Sumiteam FP-G, Shin Nippon Chemical Industry Co., Ltd.) is added to this bathase lam flour reducing solution at 1% by weight of the protein weight of the bathase lam flour and reacted at 50 ° C. for 5 hours. A liquid was obtained. The obtained enzyme-degraded Batase ram solution was subjected to ultrafiltration membrane treatment with a fractional molecular weight of 10 kDa after the enzyme was deactivated, and the concentrated solution was collected and dried by freeze-drying treatment. Milk-derived phospholipid powder was obtained.
The weight of the obtained powder is 1.1 kg, of which the lipid is 0.61 kg, the phospholipid is 0.31.kg, the lipid in the dried product is 55% by weight, and the milk-derived phospholipid is in the dried product. It contained 28% by weight, the phospholipid recovery rate of the raw material bathase lamb powder was 91%, and the yield was 22.0%.
The median diameter (volume-based cumulative 50% particle diameter) of the insoluble component when the obtained powder was made into a 5% aqueous solution was 7.3 μm as measured by a laser diffraction particle size distribution meter.

[Test Example 1]
Example 1, Example 2, Comparative Example 1, Comparative Example 2, and Comparative Example 3 to confirm the effect of the difference in median diameter of the obtained milk-derived phospholipid powder on the “roughness” texture during eating A 5% aqueous solution of the powder obtained in 1 above was prepared and subjected to sensory evaluation. Table 1 shows the sensory evaluation results. In addition, the lipid content in the dried product of the obtained powder and the phospholipid content derived from milk are shown.
The 5% aqueous solution of milk-derived phospholipid powder obtained in Example 1 and Example 2 did not feel rough because the median diameter of insoluble components was 10 μm or less. On the other hand, in the 5% aqueous solution of the milk-derived phospholipid powder obtained in Comparative Example 1, an insoluble component having a median diameter of 30 μm or more remained, so that roughness was felt. Further, the 5% aqueous solution of milk-derived phospholipid powder obtained in Comparative Examples 2 and 3 did not feel rough.

By adding 450kg of water heated to 50 ° C to 50kg of raw material butase ram (Uelzena) (0.65kg of lipid, 0.33kg of solid 5kg solids) A reducing solution was prepared. Four types of proteases having different origins were added to the reduced solution of this bathase lam powder, and reacted at 50 ° C. for 5 hours to obtain an enzyme-decomposed bathase ram solution. For the obtained enzyme-degraded batase ram solution, after inactivating the enzyme, a microfiltration membrane treatment with a fractional particle size of 1.4 μm was performed, and the concentrated solution was collected and dried by freeze-drying treatment. Milk-derived phospholipid powder was obtained.
Table 2 shows the median diameter (cumulative volume-based 50% particle diameter) of the insoluble component when the protease used in the enzyme treatment and each of the obtained powders in a 5% aqueous solution were measured by a laser diffraction particle size distribution meter. Results are shown. The milk-derived phospholipid powder prepared in this example had a median diameter of insoluble components of 10 μm or less when it was made into a 5% aqueous solution, regardless of the protease species used for the enzyme treatment. Sensory evaluation was carried out by adjusting 5% aqueous solutions of these powders, and as with the results of Example 1 and Example 2, no roughness was felt.

Batatarum reducing solution with a solid content of 10% by weight by adding 180 kg of water heated to 50 ° C to 20 kg of raw material butase lamb powder (TATUA) (of which 3.0 kg of lipid and 1.6 kg of phospholipid) Was prepared. The pH was adjusted to 4.4 by adding 10% hydrochloric acid to the Bataselam reducing solution. At the same time, calcium chloride was added to 0.02% by weight of the total amount, and then the casein was aggregated by maintaining at 50 ° C. for 30 minutes. The produced casein precipitate was completely removed by treating with a centrifuge to obtain a supernatant. The supernatant was subjected to a microfiltration membrane treatment with a fractional particle size of 1.4 μm, and the concentrated solution was recovered. Sodium hydroxide was added so that the pH of the concentrated solution was 9.5 to dissolve the remaining protein aggregates, and powder was obtained by lyophilization treatment.
The weight of the obtained powder is 2.5 kg, of which the lipid is 1.4 kg, the phospholipid is 0.7 kg, the lipid in the dried product is 56% by weight, and the milk-derived phospholipid is 28 in the dried product. % By weight. In addition, the recovery rate of phospholipids in the powder obtained from the raw material butase lamb powder was 44%, and the yield was 12%. The median diameter (volume-based cumulative 50% particle diameter) of the insoluble component when the obtained powder was made into a 5% aqueous solution was measured by a laser diffraction particle size distribution meter, and found to be 9.6 μm.
The median diameter was similar to that in Example 1, but the phospholipid recovery rate and yield were poor compared to Example 1.

Claims (10)

  The dry matter contains 45 to 70% by weight of lipid, the content of the phospholipid derived from milk contained in the lipid in the dry matter is 20% by weight or more, and the median diameter of the insoluble component when it is made into a 5% aqueous solution is Milk-derived phospholipid-containing powder, characterized by being 10 μm or less. Adjusting the starting material containing the dairy product to pH 4.0 to 5.0, adding calcium chloride to remove the generated precipitate, and obtaining a supernatant;
Concentrating the supernatant by membrane treatment;
Adjusting the pH of the concentrate to 9.0 to 10.0 to solubilize protein aggregates and drying,
A method for producing a milk-derived phospholipid-containing powder, comprising: Adding a proteolytic enzyme to the starting material containing the dairy product to break down the protein;
A step of deactivating the proteolytic enzyme after degrading the protein;
After deactivating the proteolytic enzyme, separating the degraded protein by membrane treatment to obtain a concentrated solution;
And a step of drying the concentrated solution. A method for producing a milk-derived phospholipid-containing powder.   4. The method according to claim 3, wherein the membrane treatment is a treatment by microfiltration with a fractional particle size of 1.4 [mu] m or less.   The method according to claim 3, wherein the membrane treatment is a treatment by ultrafiltration having a molecular weight cut off of 10 kDa or more.   6. The proteolytic enzyme is at least one enzyme selected from animal-derived proteases, plant-derived proteases, bacterial-derived proteases, fungal-derived proteases, and algal-derived proteases. Manufacturing method.   The method according to claim 3, wherein the proteolytic enzyme is derived from the genus Bacillus.   The production method according to claim 3, wherein the proteolytic enzyme is derived from the genus Aspergillus.   The production method according to claim 3, wherein the proteolytic enzyme is a neutral or alkaline enzyme.   The production method according to any one of claims 2 to 9, wherein the dairy product is at least one of buttermilk, buttermilk powder reducing solution, butterlam, and butterlam powder reducing solution.