JP2018008905A5

JP2018008905A5 -

Info

Publication number: JP2018008905A5
Application number: JP2016139851A
Authority: JP
Filing date: 2016-07-15
Publication date: 2019-09-19
Anticipated expiration: 2036-07-15

Description

［キクバヤマボクチ抽出物の調製］
植物原料から抽出物を調製する一般的方法を適宜採用することができるが、溶媒抽出法が望ましい。溶媒抽出については、水、エタノール等の極性溶媒を用いることが望ましく、水とその他の極性溶媒を同時に用いることがさらに望ましい。さらに、抽出物を活性炭処理、液液分配、カラムクロマトグラフィー、液体クロマトグラフィー、ゲル濾過、等によって、分離精製し、さらに活性の高い画分（成分）を抽出することもできる。
[Preparation of Kikubayama Bo Chi extract]
A general method for preparing an extract from plant raw materials can be employed as appropriate, but a solvent extraction method is desirable. For solvent extraction, it is desirable to use a polar solvent such as water or ethanol, and it is more desirable to use water and another polar solvent simultaneously. Furthermore, the extract can be separated and purified by activated carbon treatment, liquid-liquid distribution, column chromatography, liquid chromatography, gel filtration, etc., and a fraction (component) with higher activity can be extracted.

上記の通り、キクバヤマボクチエキスにＤＰＰＨラジカル消去効果が認められた。
As described above, the DPPH radical scavenging effect was observed in Kikubayama box extract.

上記の通り、キクバヤマボクチエキスのＵＶＢに起因する炎症関連分子であるTNF−α遺伝子の発現抑制効果が認められる。
As described above, the effect of suppressing the expression of the TNF-α gene, which is an inflammation-related molecule caused by UVB of Kikubayama boxy extract, is observed .

得られたdot-blotting（図１）を解析した結果、上記の通り、キクバヤマボクチエキスのglyoxalに起因するAGEs形成の抑制効果が認められた。
As a result of analysis of the obtained dot-blotting (FIG. 1), as described above, an inhibitory effect on the formation of AGEs due to glyoxal of the kikubayama boxy extract was observed.

上記の通り、キクバヤマボクチエキスの保湿効果が認められた。
As described above, the moisturizing effect of Kikubayama Box extract was recognized.

上記の通り、天然保湿因子ＮＭＦの増加によるバリア機能が向上の指標となるclaudin-1（CLDN1）およびfilaggrin（FLG）の増加が認められた。
As described above, an increase in claudin-1 (CLDN1) and filaggrin (FLG), which are indicators for improving the barrier function due to an increase in natural moisturizing factor NMF, was observed.

得られたdot-blotting（図２）を解析した結果、上記の通り、UVB起因する皮膚老化度合の指標である変性タンパク質の一種4-HNEMPの形成抑制効果が認められた。
As a result of analyzing the obtained dot-blotting (FIG. 2), an effect of inhibiting formation of 4-HNEMP, a kind of denatured protein, which is an index of the degree of skin aging caused by UVB was observed as described above.

［化粧水］
実施例１のキクバヤマボクチエキスを使用して以下の構成成分を常法で調製した。（数値は重量％）
また、比較例として、キクバヤマボクチエキス分を精製水で置換したものを調製した。
[Lotion]
The following constituents were prepared in a conventional manner using the Kikubayama box extract of Example 1. (Number is weight%)
In addition, as a comparative example, a product obtained by substituting Kikubayama box extract with purified water was prepared.

［ローションタイプ］
実施例１のキクバヤマボクチエキスを使用して以下の構成成分を常法で調製した。（数値は重量％）
また、比較例として、キクバヤマボクチエキス分を精製水で置換したものを調製した。
[Lotion type]
The following constituents were prepared in a conventional manner using the Kikubayama box extract of Example 1. (Number is weight%)
In addition, as a comparative example, a product obtained by substituting Kikubayama box extract with purified water was prepared.

［クリーム剤］
実施例１のキクバヤマボクチエキスを使用して以下の構成成分を常法で調製した。（数値は重量％）
また、比較例として、キクバヤマボクチエキス分を精製水で置換したものを調製した。
[Cream]
The following constituents were prepared in a conventional manner using the Kikubayama box extract of Example 1. (Number is weight%)
In addition, as a comparative example, a product obtained by substituting Kikubayama box extract with purified water was prepared.

［パック剤］
実施例１のキクバヤマボクチエキスを使用して以下の構成成分を常法で調製した。（数値は重量％）
また、比較例として、キクバヤマボクチエキス分を精製水で置換したものを調製した。
[Packing agent]
The following constituents were prepared in a conventional manner using the Kikubayama box extract of Example 1. (Number is weight%)
In addition, as a comparative example, a product obtained by substituting Kikubayama box extract with purified water was prepared.

上記の通り、キクバヤマボクチエキスの抗皮膚老化剤及びこれを含む化粧料の抗皮膚老化効果が認められた。

As above-mentioned, the anti-skin aging effect of the anti-skin aging agent of Kikubayama bokuchi extract and the cosmetics containing this was recognized.