JP2017198486A - がん診断装置、がん診断方法、及びプログラム - Google Patents
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Abstract
Description
対象生体組織から生成した懸濁液の蛍光強度の測定結果を用いて、蛍光強度と細胞数の関係を示すヒストグラムを生成するヒストグラム生成部と、
前記ヒストグラムを解析して前記対象生体組織のがん種別の判定を行う判定部と、を備え、
前記判定部は、
前記ヒストグラムの細胞数のピークに対応する蛍光強度に基づいてS期細胞に対応するS期蛍光強度範囲を検出し、
前記S期蛍光強度範囲の細胞数の変化を基に、DNA異数性が生じている腫瘍細胞に対応する異常蛍光強度範囲を検出し、
前記S期蛍光強度範囲から前記異常蛍光強度範囲を除外した範囲の細胞数の指標に基づいて前記対象生体組織のがん診断を行う、ものである。
以下、図面を参照して本発明の実施の形態について説明する。図1は、本実施の形態にかかるがん診断装置1の外観例を示す斜視図である。なお図1に示すがん診断装置1の外観はあくまでも一例であり、この他の形状であっても勿論構わない。がん診断装置1は、フローサイトメトリ(Flow Cytometry)に用いられる装置である。フローサイトメトリとは、微細な粒子を流体中に分散させ、その液体(懸濁液)を流して個々の粒子を光学的に分析する手法である。がん診断装置1は、がん診断の対象となる生体組織(以下、対象生体組織とも呼称する。)の細胞核染色された細胞数を計測し、その計測結果を用いて細胞数と蛍光強度(DNA量)の関係を示すDNAヒストグラムを生成する。がん診断装置1は、DNAヒストグラムを用いたがん解析を行う。
2 細胞単離装置
11 ノズル
12 細胞前処理部
13 光学処理部
14 ヒストグラム生成部
15 判定部
16 出力部
20 ピペット部材
21 本体
22 フィルタ
23 蓋体
24 容器
25 試薬
Claims (8)
- 対象生体組織から生成した懸濁液の蛍光強度の測定結果を用いて、蛍光強度と細胞数の関係を示すヒストグラムを生成するヒストグラム生成部と、
前記ヒストグラムを解析して前記対象生体組織のがん診断を行う判定部と、を備え、
前記判定部は、
前記ヒストグラムの細胞数のピークに対応する蛍光強度に基づいてS期細胞に対応するS期蛍光強度範囲を検出し、
前記S期蛍光強度範囲の細胞数の変化を基に、DNA異数性が生じている腫瘍細胞に対応する異常蛍光強度範囲を検出し、
前記S期蛍光強度範囲から前記異常蛍光強度範囲を除外した範囲の細胞数の指標に基づいて前記対象生体組織のがん診断を行う、がん診断装置。 - 前記がん診断は、がんの種別の判定又は悪性度の判定であることを特徴とする請求項1に記載のがん診断装置。
- 前記判定部は、前記S期蛍光強度範囲から前記異常蛍光強度範囲を除外した範囲の細胞数の代表値と、前記ヒストグラムのピーク値と、を比較して前記対象生体組織のがん診断を行う、ことを特徴とする請求項1又は2に記載のがん診断装置。
- 前記代表値は、平均値、または最頻値、または中央値である、ことを特徴とする請求項3に記載のがん診断装置。
- 前記判定部は、前記S期蛍光強度範囲から前記異常蛍光強度範囲を除外した範囲の曲線下面積と、前記ヒストグラムの曲線下面積と、を比較して前記対象生体組織のがん診断を行う、ことを特徴とする請求項1又は2に記載のがん診断装置。
- 前記判定部は、前記S期蛍光強度範囲から前記異常蛍光強度範囲を除外した範囲の曲線下面積と、前記ヒストグラムの全範囲からデブリに相当する範囲を除外した範囲の曲線下面積と、を比較して前記対象生体組織のがん診断を行う、ことを特徴とする請求項1又は2に記載のがん診断装置。
- 対象生体組織から生成した懸濁液の蛍光強度の測定結果を用いて、蛍光強度と細胞数の関係を示すヒストグラムを生成するヒストグラム生成ステップと、
前記ヒストグラムを解析して前記対象生体組織のがん診断を行う判定ステップと、を備え、
前記判定ステップでは、
前記ヒストグラムの細胞数のピークに対応する蛍光強度に基づいてS期細胞に対応するS期蛍光強度範囲を検出し、
前記S期蛍光強度範囲の細胞数の変化を基に、DNA異数性が生じている腫瘍細胞に対応する異常蛍光強度範囲を検出し、
前記S期蛍光強度範囲から前記異常蛍光強度範囲を除外した範囲の細胞数の指標に基づいて前記対象生体組織のがん診断を行う、がん診断方法。 - コンピュータに、
対象生体組織から生成した懸濁液の蛍光強度の測定結果を用いて、蛍光強度と細胞数の関係を示すヒストグラムを生成するヒストグラム生成ステップと、
前記ヒストグラムを解析して前記対象生体組織のがん種別の判定を行う判定ステップと、を実行させ、
前記判定ステップでは、
前記ヒストグラムの細胞数のピークに対応する蛍光強度に基づいてS期細胞に対応するS期蛍光強度範囲を検出し、
前記S期蛍光強度範囲の細胞数の変化を基に、DNA異数性が生じている腫瘍細胞に対応する異常蛍光強度範囲を検出し、
前記S期蛍光強度範囲から前記異常蛍光強度範囲を除外した範囲の細胞数の指標に基づいて前記対象生体組織のがん診断を行う、プログラム。
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