JP2017176047A - Methods of manufacturing containers - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide production methods of producing a container in which a functional membrane is arranged also in the corner of the bottom surface of the container.SOLUTION: The present invention provides a production method of producing a container 10 for containing cells using a female mold 46 in which a crevice 48 specifying the outer shape of the container is formed; and a male mold 41 which has a core 43 arranged in the crevice and which forms a cavity cy for molding a container between the male mold and the female mold. The production method comprises filling the cavity with a molding material in a state where a base material 13 having on the surface of one side a functional membrane 20 having a function to the cells is contained in the cavity. The base material is contained within the cavity such that a part of the functional membrane covers a tip surface 44 of the core while at least a portion of the edge 21 of the functional membrane is estranged from the tip surface and positioned in the cavity.SELECTED DRAWING: Figure 2

Description

  The present invention relates to a method for producing a container for containing cells, and more particularly to a method for producing a container having a functional membrane having a function for cells.

  For example, as shown in Utility Model Document 1, containers that contain cells are used. In such a container, a functional film having some function for cells to be accommodated may be provided on the bottom surface. In Utility Model Document 1, a hydrophilic film for imparting hydrophilicity is provided on the bottom surface of the container.

  Utility Model Document 1 proposes that a container is manufactured by in-mold molding using a functional film prepared in advance for the purpose of stably forming the functional film over the entire bottom surface of the container. In the manufacturing method disclosed in Utility Model Document 1, the molten resin is supplied into the cavity in a state where the front end surface of the core that defines the bottom surface of the container is covered with a functional film.

Japanese Utility Model Publication No. 5-88299

  By using in-mold molding, the functional film can be generally disposed on the bottom surface of the container. However, the cells accommodated in the container are fine, and it is necessary to position the functional film on the bottom surface of the container with high accuracy. In other words, in the container disclosed in Utility Model Document 1, it is expected that the functional film is not arranged with sufficient accuracy, and typically, the functional film does not sufficiently extend to the corner of the bottom surface of the container. In this case, due to the manufacturing accuracy of the container, the desired treatment cannot be sufficiently performed on the cells located on the corners of the bottom surface of the container. In particular, considering that processing of extremely expensive cell sheets or the like can be performed in the container, it can be said that it is a serious problem to spread the functional film to the corners of the bottom surface of the container.

  The present invention has been made in consideration of such points, and an object of the present invention is to provide a method of manufacturing a container in which a functional film is arranged so that it can function effectively at the corners of the bottom surface of the container. To do.

The method for producing a container according to the present invention comprises:
In order to mold a container for containing cells between a female mold in which a recess defining an outer shape of the container is formed and a female mold having a core disposed in the recess. A male mold that forms a cavity of
A step of filling the cavity with a molding material in a state where a base material having a functional membrane having a function for cells on one side is accommodated in the cavity;
The base material is formed in the cavity such that a part of the functional film covers the tip surface of the core and at least a part of an edge of the functional film is located in the cavity apart from the tip surface. Be contained.

  In the method for manufacturing a container according to the present invention, the base material is configured such that the functional film covers the entire area of the tip surface of the core, and the edge of the functional film is separated from the tip surface on the entire circumference. You may accommodate in the said cavity so that it may be located in the said cavity.

  The method for producing a container according to the present invention further includes a step of filling the cavity of the preform with a molding material in a state where the functional film is accommodated in the cavity of the preform, and molding the base material. You may make it prepare.

  According to the present invention, it is possible to manufacture a container in which the functional membrane can function effectively even at the corners of the bottom surface of the container.

FIG. 1 is a perspective view showing a container for explaining an embodiment of the present invention. FIG. 2 is a longitudinal sectional view of the container of FIG. FIG. 2A is a longitudinal sectional view corresponding to FIG. 2 and showing a modified example of the container. FIG. 3 is a plan view showing the bottom of the container of FIG. FIG. 4 is a view for explaining a method of manufacturing the container of FIG. FIG. 5 is a view for explaining a method of manufacturing the container of FIG. FIG. 6 is a view for explaining a method of manufacturing the container of FIG. FIG. 7 is a view for explaining a method of manufacturing the container of FIG. 8 is a perspective view corresponding to FIG. 1 and showing another modified example of the container. FIG. 9 is a perspective view corresponding to FIG. 1 and showing still another modified example of the container. FIG. 10 is a view corresponding to FIG. 2 and a longitudinal sectional view of the container of FIG. FIG. 11 is a view corresponding to FIG. 6 and illustrating the method for manufacturing the container of FIG. FIG. 12 is a view corresponding to FIG. 10 for explaining another example of the method for manufacturing the container of FIG.

  Hereinafter, an embodiment of the present invention will be described with reference to the drawings. In the drawings attached to the present specification, for the sake of illustration and ease of understanding, the scale, the vertical / horizontal dimension ratio, and the like are appropriately changed and exaggerated from those of the actual product.

  1-12 is a figure for demonstrating one Embodiment and its modification of this invention. Among these, FIG.1 and FIG.2 is a perspective view or longitudinal cross-sectional view which respectively shows a container, FIG. 3 is a top view which cuts out and shows the bottom part of a container.

  As shown in FIGS. 1 and 2, the container 10 has a bottom portion 11 and a side wall portion 12. The container 10 has a bottom surface 10 a formed by the bottom portion 11, and an inner wall surface 10 b and an outer wall surface 10 c formed by the side wall portion 12. The container 10 has a storage space S defined by a bottom surface 10a and an inner wall surface 10b. The container 10 is intended to store cells in the storage space S thereof. At least a part of the bottom surface 10a of the container 10 is formed by a functional film 20 that can exhibit a predetermined function with respect to the contained cells. In particular, in the container manufacturing method described below, a contrivance is made to reliably form the functional film 20 up to the corner of the bottom surface 10a that defines the boundary with the inner wall surface 10b. That is, according to the container 10 manufactured with the manufacturing method demonstrated below, the effect | action resulting from the function of the functional film 20 can be exerted effectively with respect to a cell.

  First, the configuration of the container 10 will be described. As described above, the container 10 has the bottom part 11 and the side wall part 12. In addition, the bottom part 11 and the side wall part 12 are division according to a site | part and do not necessarily correspond to the division based on a manufacturing process, a boundary, or a boundary so that it may become clear also from description of the manufacturing method mentioned later. For example, the interface is formed during the manufacturing steps that are sequentially performed may be located in the bottom 11 as shown in FIG. 2A.

  As shown in FIG. 2, the bottom part 11 has the 1st surface 11a and the 2nd surface 11b which were formed in plate shape and mutually opposed. The first surface 11a is a surface facing upward when the container 10 is used, and is a flat surface. The second surface 11b is a surface facing downward when the container 10 is used. The first surface 11a forms a bottom surface 10a of the container 10 in a part thereof. A side wall 12 is provided on the first surface 11 a of the bottom 11. As shown in FIG. 2, the side wall portion 12 rises from the first surface 11 a of the bottom portion 11. In the example shown in FIG. 1, the side wall portion 12 is formed in a circumferential shape on the first surface 11a. The side wall portion 12 forms an inner wall surface 10b as an inner surface with reference to the circumference formed by the side wall portion 12. Moreover, the side wall part 12 forms the outer wall surface 10c as a surface which becomes the outer side on the basis of the circumference which the side wall part 12 forms.

  Here, FIG. 3 is a plan view showing the bottom portion 11 from the first surface 11a side with the side wall portion 12 omitted. A dotted line in FIG. 3 indicates a position connected to the inner wall surface 10b of the first surface 11a. As shown in FIG. 3, the first surface 11 a includes a peripheral region ca covered with the side wall portion 12 and a bottom surface region ba surrounded by the peripheral region ca. In the illustrated example, the bottom surface region ba is a region surrounded by the side wall portion 12 and forms the bottom surface 10 a of the container 10. In the illustrated example, the peripheral area ca is the peripheral edge of the first surface 11a.

  Returning to FIG. 2, the bottom 11 has the functional film 20 on the first surface 11 a. That is, the functional film 20 forms at least a part of the first surface 11a. Further, as shown in FIGS. 2 and 3, the functional film 20 is provided across the peripheral area ca and the bottom area ba. Therefore, in the part where the functional film 20 extends over the peripheral region ca and the bottom surface region ba, the functional film 20 extends and spreads reliably to the corner of the bottom surface 10a that becomes the boundary with the inner wall surface 10b. In the illustrated example, the functional film 20 forms the first surface 11a in the entire bottom region ba. Further, the edge portion 21 of the functional film 20 is located in the peripheral region ca over the entire circumference. That is, the edge 21 of the functional film 20 is located between the inner edge and the outer edge of the peripheral region ca. In other words, the edge 21 of the functional film 20 is located between the position connected to the inner wall surface 10b and the position connected to the outer wall surface 10c of the first surface 11a. Therefore, in the illustrated example, the bottom surface 10a of the container 10 is formed by the functional film 20 of the bottom portion 11, and further extends and extends to all corners of the bottom surface 10a.

  In the container 10 configured as described above, the portion other than the functional film 20 of the bottom portion 11 and the side wall portion 12 are formed of, for example, a resin material. As resin materials that can be used, polystyrene resin, polyester resin, polyethylene resin, polyethylene terephthalate resin, polypropylene resin, ABS resin, nylon, acrylic resin, fluororesin, polycarbonate resin, polyurethane resin, methylpentene resin, phenol resin, melamine resin, Examples thereof include resin materials such as epoxy resins and vinyl chloride resins, and resin materials containing at least one of the above-described surface hydrophilized treatments. In addition, in order to enable observation of the cells accommodated in the container 10, the bottom part 11 and the side wall part 12 are preferably transparent. Further, the bottom portion 11 and the side wall portion 12 may be formed integrally so that the interface is not observed between them, or may be formed as a separate body and joined, and the interface may be observed between them. Good.

Next, the functional film 20 will be described. As described above, the functional membrane 20 is a membrane that is expected to exhibit a predetermined function with respect to the contained matter such as cells contained in the container 10. The function expected of the functional film 20 is not particularly limited, but as an example, a stimulus-responsive polymer having a surface that can be changed from cell-adhesive to non-cell-adhesive by a predetermined stimulus, one or more The functional film 20 can be formed as a functional organic compound film using a hydrophilic compound such as an ethylene glycol chain composed of an ethylene glycol unit (CH ₂ —CH ₂ —O). The film thickness of the functional film 20 is, for example, preferably in the range of 0.5 nm to 300 nm, and more preferably in the range of 1 nm to 100 nm.

  Hereinafter, preferred embodiments of the “stimulus responsive polymer film” and the “hydrophilic compound film” will be described.

<Stimulus-responsive polymer film>
The stimulus-responsive polymer film is a film containing a polymer whose degree of adhesion to cells on the surface is changed by a predetermined stimulus. That is, examples of the stimulation-responsive polymer film include a film that changes the adhesion with cells depending on the presence or absence of stimulation. Such a stimulus-responsive polymer film can be applied to the container 10 intended to produce a cell sheet by culturing cells on the bottom surface 10a. In this application, the cell sheet can be produced in a state of being adhered on the bottom surface 10 a of the container 10. The produced cell sheet is very susceptible to damage. Therefore, when the cell sheet is taken out from the container 10, there is a possibility that the cell sheet may be damaged such as tearing depending on the magnitude and variation of the adhesive strength to the bottom surface 10 a. When a stimulus-responsive polymer film capable of changing the adhesion of the container bottom surface 10a to the cell sheet is used, the cell sheet can be stably produced in the container 10, and damage such as tearing can be caused. The cell sheet can be taken out from the container 10 while avoiding it.

  Examples of the stimulus responsive polymer include a temperature responsive polymer, a pH responsive polymer, an ion responsive polymer, and a photoresponsive polymer. Among these, a temperature-responsive polymer is preferable because it is easy to give a stimulus.

  As the temperature-responsive polymer, for example, a polymer that exhibits cell adhesiveness at a temperature when cells are cultured and exhibits cell non-adhesiveness at a temperature when the produced cell sheet is peeled may be used. For example, a temperature-responsive polymer has an improved affinity for surrounding water at temperatures below the critical dissolution temperature, and the property that the polymer takes up water and swells to make it difficult for cells to adhere to the surface, that is, cell non-adhesiveness. It is preferable to use a material that exhibits a property of facilitating the adhesion of cells to the surface by desorption of water from the polymer at a temperature equal to or higher than the critical dissolution temperature, that is, cell adhesion. Such a critical solution temperature is called a lower critical solution temperature. A temperature-responsive polymer having a lower critical solution temperature T of 0 ° C. to 80 ° C., more preferably 0 ° C. to 50 ° C. may be used. This is because the cells can be stably cultured when T is 0 ° C to 80 ° C. It is also possible to form the functional film 20 using a temperature-responsive polymer that exhibits cell non-adhesion at a temperature equal to or higher than the critical lysis temperature and exhibits cell adhesion at a temperature lower than the critical lysis temperature.

  Suitable temperature-responsive polymers include acrylic polymers or methacrylic polymers. Specific examples of suitable temperature-responsive polymers include poly-N-isopropylacrylamide (T = 32 ° C.), poly-Nn-propyl acrylamide (T = 21 ° C.), and poly-Nn-propyl methacrylamide. (T = 32 ° C.), poly-N-ethoxyethyl acrylamide (T = about 35 ° C.), poly-N-tetrahydrofurfuryl acrylamide (T = about 28 ° C.), poly-N-tetrahydrofurfuryl methacrylamide (T = About 35 ° C.), and poly-N, N-diethylacrylamide (T = 32 ° C.).

  As a monomer for forming these polymers, a monomer that can be polymerized by irradiation with radiation can be used. Examples of the monomer include (meth) acrylamide compounds, N- (or N, N-di) alkyl-substituted (meth) acrylamide derivatives, (meth) acrylamide derivatives having a cyclic group, vinyl ether derivatives, and the like. The above may be used. When a single monomer is used alone, the polymer formed is a homopolymer, and when multiple monomers are used together, the polymer is a heteropolymer, but either form is possible.

  In addition, when it is necessary to adjust T depending on the type of proliferating cell, when it is necessary to enhance the interaction between the coating substance and the cell culture support, and the balance between the hydrophilicity and hydrophobicity of the cell support is adjusted. If necessary, other monomers other than those described above may be further added for copolymerization. Further, a graft or block copolymer of the above polymer and another polymer, or a mixture of the above polymer and another polymer may be used. Moreover, it is also possible to crosslink within a range where the original properties of the polymer are not impaired.

  As the pH responsive polymer and the ion responsive polymer, those suitable for the cells accommodated in the container 10 can be appropriately selected.

  The stimulus-responsive polymer film is prepared by preparing a coating composition containing a monomer that is polymerized to form a target stimulus-responsive polymer and an organic solvent that can dissolve the monomer. The film is applied to the surface of the substrate to form a coating film, and then the monomer in the coating film is polymerized by an appropriate means such as radiation irradiation to form a polymer. It can be formed by causing a grafting reaction in between.

<Hydrophilic compound layer>
Another example of the functional film 20 is a hydrophilic compound such as an ethylene glycol chain composed of one or more ethylene glycol units (an ethylene glycol chain composed of a plurality of ethylene glycol units can be referred to as a “polyethylene glycol chain”). Layer. The terminal of the ethylene glycol chain may be in a form blocked with a hydroxyl group, or the terminal of the ethylene glycol chain may be in a form in which another substance such as a biological substance is linked by a covalent bond. A layer containing an ethylene glycol chain whose end is blocked with a hydroxyl group can provide a hydrophilic surface to which cells are difficult to adhere.

  Examples of biological substances that can be covalently bonded to the end of an ethylene glycol chain include antigens, antibodies, DNA, RNA, peptides, hormones, enzymes, cytokines, sugar chains, lipids, coenzymes, enzyme inhibitors, cells, and other functions. The protein which has is included. Furthermore, the low molecular weight compound which has affinity with such a biological substance, and a high molecular compound are also contained in the range of a biological substance.

  In order to immobilize a layer of a hydrophilic compound such as an ethylene glycol chain on the surface of the support material, it can be physically adsorbed on the surface of the support material in advance, and the end of the ethylene glycol chain can be immobilized. An underlayer containing polysiloxane containing a functional group capable of reacting with a hydroxyl group to form a covalent bond in the side chain is provided. The functional group on the side chain of the polysiloxane is preferably a glycidyl group or an epoxy group. The underlayer can be formed by applying a silanol compound having a desired side chain to the surface of the support material, and performing condensation polymerization on the surface to convert it into polysiloxane.

  Next, the functional group of the primer layer is reacted with a hydroxyl group of polyethylene glycol in which two or more ethylene glycol or ethylene glycol units are repeated to form a covalent bond, thereby immobilizing the ethylene glycol chain. At this time, ethylene glycol or polyethylene glycol containing a catalytic amount of concentrated sulfuric acid is brought into contact with the primer layer. A layer containing ethylene glycol chains whose ends are blocked with hydroxyl groups is formed in this way.

  Furthermore, if necessary, at least one functional group capable of forming a covalent bond with another substance is directly or indirectly linked to one end of the ethylene glycol chain. The method for introducing the functional group is not particularly limited.

  Next, an example of a method for manufacturing the container 10 having the above configuration will be described. In the manufacturing method described below, the substrate 13 is formed by in-mold molding using the functional substrate 15 in the first step of forming the functional substrate 15 by forming the functional film 20 on the support layer 16. It has the 2nd process to produce, and the 3rd process to produce container 10 by in-mold molding using base material 13.

  First, the first step for producing the functional substrate 15 shown in FIG. 4 will be described. The illustrated functional substrate 15 includes a functional film 20 and a support layer 16 that supports the functional film 20. The support layer 16 functions as a support material when the functional film 20 is produced.

  Specifically, the formation of the functional film 20 on the support layer 16 can be performed as follows. First, a composition that forms the functional film 20 by being cured or dried is applied on the support layer 16. The application method is not particularly limited, and various methods such as bar coating, roll coating, and gravure coating can be used. Next, the solidification process of the coating film on the support layer 16 is implemented as needed. For the solidification treatment, a method according to the composition of the coating film is employed, and as an example, the coating process is performed by electron beam irradiation on the coating film. Then, the thickness of a coating film is adjusted by spray-cleaning a coating film. In order to smoothly adjust the thickness, the coating film may be immersed in water together with the support layer 16 and swollen before spray cleaning. Finally, by drying the coating film, the functional substrate 15 having the support layer 16 and the functional film 20 made of the coating film can be obtained.

  The “base” may be a film or a plate as long as it has a predetermined structure. The film-like functional substrate 15 is preferably flexible so that it can be wound into a roll. The flexible functional substrate 15 is preferable because mass production by a roll-to-roll method is easy. One or more other layers (for example, the above-described underlayer) may be present between the support layer 16 and the functional film 20 as necessary. The shape of the functional substrate 15 in plan view can be an arbitrary shape according to the shape of the bottom surface 10 a of the container 10. For example, it may be a polygon such as a triangle, a rectangle (rectangle, square, parallelogram, rhombus, etc.), a pentagon, a hexagon, a heptagon, an octagon, a circle, an ellipse, or the like.

  The support layer 16 is appropriately selected according to the final functional substrate 15. If the functional substrate 15 is plate-shaped, a plate-shaped support material is used as the support layer 16, and if the functional substrate 15 is film-shaped, a film-shaped support material is used as the support layer 16.

  The material of the support layer 16 can be appropriately selected from resin materials that can form the functional film 20 on one surface, but the type of the material is not particularly limited. Typically, as the material of the support layer 16, polyethylene terephthalate (PET), polystyrene (PS), polycarbonate (PC), TAC (triacetyl cellulose), polyimide (PI), nylon (Ny), low density polyethylene (LDPE) ), Medium density polyethylene (MDPE), polyvinyl chloride, polyvinylidene chloride, polyphenylene sulfide, polyether sulfone, polyethylene naphthalate, polypropylene, acrylic resin, and the like.

  The surface of the support layer 16 on the side on which the functional film 20 is formed can be a surface subjected to an easy adhesion treatment. “Easy adhesion treatment” refers to treatment with an easy adhesive such as polyester, acrylic ester, polyurethane, polyethyleneimine, silane coupling agent, perfluorooctane sulfonic acid (PFOS), and the like.

  The thickness of the support layer 16 can be selected as appropriate. When the support layer 16 is a film material, the thickness is not particularly limited, but may be, for example, 5 to 500 μm, more preferably 20 to 500 μm, and particularly preferably 50 to 250 μm.

  Next, the 2nd process which produces the base material 13 by the in-mold shaping | molding using the functional base | substrate 15 is demonstrated.

  The second step is performed using the preforming die 30 shown in FIG. The preforming mold 30 has a first mold 31 and a second mold 36. The first mold 31 and the second mold 36 form a cavity cx corresponding to the outer shape of the base material 13 to be manufactured in a state where the molds are clamped close to each other. In the illustrated example, the first mold 31 has a plate-like outer shape. The first mold 31 is formed with a suction hole 32 communicating with the suction means. On the other hand, the second mold 36 has a recess 38 that can accommodate the functional substrate 15. In particular, in the illustrated example, the recess 38 includes a first recess 38 a located on the first mold 31 side and a second recess 38 b located on the side away from the first mold 31. The opening shape of the second recess 38b is smaller than the opening shape of the first recess 38a. That is, the recess 38 has a stepped portion 38c that defines the periphery of the second recess 38b therein, and is tapered stepwise. The second mold 36 is formed with an injection hole 37 leading to the molding material source. The injection hole 37 communicates with the second recess 38 b of the recess 38.

  A 2nd process produces the base material 13 by in-mold shaping | molding as a preforming process. First, as shown in FIG. 5, the functional substrate 15 is disposed on the first mold 31 so as to block the suction holes 32. At this time, the functional film 20 of the functional substrate 15 is made to face the suction hole 32. The functional substrate 15 is fixed to the first mold 31 by suction through the suction holes 32. In this state, the first mold 31 and the second mold 36 are clamped to reach the state shown in FIG.

  In the illustrated example, the shape of the functional base 15 including the functional film 20 is smaller than the opening shape of the first recess 38 a of the recess 38 of the second mold 36. Accordingly, the functional base 15 is positioned between the first mold 31 and the second mold 36 when the preform 30 is clamped without positioning the functional base 15 with respect to the first mold 31 with extremely high accuracy. Can be effectively prevented from being crushed. That is, positioning of the functional base 15 with respect to the first mold 31 can be performed easily and quickly.

  On the other hand, the shape of the functional substrate 15 including the functional film 20 is larger than the opening shape of the second recess 38 b of the recess 38 of the second mold 36. In the mold clamping state shown in FIG. 5, the peripheral edge portion of the functional base 15 is located in the first recess 38 a of the recess 38 that defines the cavity cx of the preform 30. In the mold clamping state shown in FIG. 5, the functional base 15 sandwiches the peripheral edge between the first mold 31 and the stepped portion 38 c of the second mold 36 in the cavity cx of the preforming mold 30. Has been. As a result, the functional substrate 15 is stably held in the cavity cx of the preforming die 30. The depth of the first recess 38a of the recess 38 is such that the functional base 15 can be accommodated without damage, and the depth of the second recess 38b is the thickness of the support 14 to be manufactured. Is determined in consideration of

  Next, the molding material is filled into the cavity cx of the preforming die 30 through the injection hole 37. The molding material can be the material already described as the material that forms the side wall portion 12 and the portion other than the functional film 20 of the bottom portion 11. When the molding material is injected into the cavity cx, the functional substrate 15 is held in a state where the entire surface of the functional film 20 is in contact with the first mold 31. In the illustrated example, the periphery of the functional substrate 15 is sandwiched between the first mold 31 and the second mold 36. Therefore, even if a high-temperature and high-pressure molding material, typically a high-temperature and high-pressure resin material, is injected into the cavity cx of the preforming die 30, the functional film 20 is damaged or the functional film 20 is broken or wrinkled. This can be effectively prevented.

  When the molding material injected into the cavity cx of the preforming mold 30 is solidified due to a decrease in temperature, the support 14 made of the molding material is formed. As described above, the substrate 13 having the support 14 and the functional substrate 15 supported on the support 14 can be obtained.

  In order to improve the adhesion between the support 14 and the functional substrate 15, the surface of the portion of the support layer 16 of the functional substrate 15 that is integrated with the support 14 contains a heat-sealable resin material. You may go out. The heat-sealable resin material is melted by contact with a high-temperature molten resin and solidifies as the resin is solidified. For this reason, the bonding between the functional base 15 and the support 14 can be further strengthened.

  Moreover, you may make it the base material 13 produced as mentioned above remove the peripheral part by stamping. For example, the base material 13 is removed at a portion including the peripheral edge portion of the functional substrate 15 sandwiched between the first mold 31 and the second mold 36. By such removal, the support 14 and the functional base 15 may be flush with each other at the peripheral edge of the base material 13 as shown in FIG.

  Next, the 3rd process which produces the container 10 by the in-mold shaping | molding using the base material 13 is demonstrated.

  The third step is performed using the mold 40 shown in FIG. The molding die 40 has a male die 41 and a female die 46. The male mold 41 and the female mold 46 form a cavity cy when they are close to each other and clamped. The cavity cy defines an internal space corresponding to the outer shape of the container 10. In the illustrated example, the female mold 46 has a recess 48 corresponding to the outer shape of the container 10 to be produced. On the other hand, the male mold 41 has a core 43 disposed in the recess 48. A region surrounded by the recess 48 and the core 43 forms a cavity for molding the container 10. The male mold 41 is formed with a suction hole 42 communicating with the suction means. The female mold 46 is formed with an injection hole 47 leading to the molding material source. The injection hole 47 communicates with the recess 48.

  In the third step, the container 10 is produced by in-mold molding. First, as shown in FIG. 6, the base material 13 is arranged on the male mold 41 so as to close the suction hole 42. At this time, a part of the functional film 20 included in the base material 13 covers the tip surface 44 of the core 43. A suction hole 42 is opened in the distal end surface 44. Accordingly, the base material 13 is fixed to the male mold 41 by suction through the suction holes 42. At this time, as shown in FIGS. 6 and 7, at least a part of the edge 21 of the functional film 20 extends beyond the edge 44 a of the tip surface 44 of the core 43 and extends into the cavity cy. That is, the functional film 20 is exposed in the cavity cy at least partially without covering the tip surface 44. In particular, in the illustrated example, the part of the functional film 20 that is separated from the core front end surface 44, in other words, the part that is exposed in the cavity cy of the functional film 20 is located on the extended surface of the front end surface 44. In other words, in the illustrated example, the portion of the functional film 20 that is separated from the core tip surface 44 is located in the same plane as the tip surface 44 of the core 43 in the cavity cy. As will be described later, it is important for the functional film 20 to extend into the cavity cy at least partially away from the distal end surface 44 of the core 43, while the male film of such a functional film 20 is provided. The positioning with respect to the mold 41 is compared with the positioning disclosed in Japanese Utility Model Laid-Open No. 5-88299, that is, the functional film having the same shape as the tip surface of the core is positioned so that the tip surface matches the outer shape. Can be implemented easily and quickly.

  FIG. 7 is a plan view showing the positional relationship between the tip surface 44 of the core 43, the functional film 20, the base material 13, and the recess 48. FIG. In the illustrated example, the functional film 20 has a larger area than the front end surface 44 of the core 43. The functional film 20 covers the entire tip surface 44 of the core 43. The edge portion 21 of the functional film 20 extends into the cavity cy over the edge portion 44a of the distal end surface 44 on the entire circumference. Such positioning can be carried out easily and extremely quickly as compared with the positioning disclosed in Japanese Utility Model Laid-Open No. 5-88299.

  Further, in the illustrated example, the shape of the base material 13 including the functional film 20 is smaller than the opening shape of the concave portion 48 of the female mold 46. In other words, in the state shown in FIGS. 6 and 7, the functional film 20 is held in the cavity cy of the mold 40 while being separated from the female mold 46. Therefore, the base material 13 is sandwiched between the male mold 41 and the female mold 46 when the mold 40 is clamped without positioning the base material 13 with respect to the male mold 41 with extremely high accuracy. It can be effectively avoided. Also from this point, the base material 13 can be easily and quickly positioned with respect to the male mold 41.

  After the mold 40 is clamped, the molding material is filled into the cavity cy of the mold 40 through the injection hole 47. The molding material can be the material already described as the material that forms the side wall portion 12 and the portion other than the functional film 20 of the bottom portion 11. When the molding material is injected into the cavity cy, the base material 13 is adsorbed and held on the front end surface 44 of the core 43. However, the peripheral edge portion of the base material 13 including the edge portion 21 of the functional film 20 is separated from the tip surface 44 and extends into the cavity cy. Therefore, the peripheral portion of the substrate 13 is exposed to a molding material that flows at high temperature and pressure. However, the base material 13 is a so-called injection molded product and can have a sufficient thickness. For this reason, even if the base material 13 is pressed by the high-temperature and high-pressure molding material, it can effectively avoid deformation such as wrinkles and bending and damage such as cracks to the peripheral portion extending into the cavity cy. it can. Accordingly, it is possible to effectively prevent the molding material from flowing and mixing between the front end surface 44 of the core 43 and the base material 13.

  The molding material injected into the cavity cy of the molding die 40 is solidified due to a decrease in temperature, whereby the molding material is integrated with the base material 13 to obtain the container 10. The manufactured container 10 has a bottom part 11 and a side wall part 12. In the container 10 manufactured through the third step shown in FIG. 6, the material injected in the second step to form the support 14 of the substrate 13 and the bottom portion injected in the third step. The interface is shown in FIG. 2A is observed due to the relationship between a part of 11 and the material forming the side wall 12.

  In in-mold molding using the molding die 40, it is effective to prevent the molding material from entering between the core 43 and the base material 13 by preventing the deformation of the base material 13 extending from the tip surface 44 of the core 43. Can be prevented. As a result, in the obtained container 10, as shown in FIGS. 1 to 3, the functional film 20 is exposed to the corner of the container bottom surface 10 a. In particular, when molding is performed in the positional relationship shown in FIG. 7, the container bottom surface 10 a is formed by the functional film 20 until it covers all corners. At this time, at least a part of the side wall 12 of the container 10 is provided on the functional film 20.

  From the viewpoint of preventing deformation of the base material 13 during in-mold molding using the molding die 40, the thickness t (see FIG. 6) of the base material 13 is preferably 0.1 mm or more and 10 mm or less. More preferably, it is 5 mm or more and 5 mm or less. Further, from the viewpoint of surely extending the functional film 20 to the corner of the container bottom surface 10a and from the viewpoint of sufficiently facilitating the positioning of the base material 13 with respect to the core 43, the extension length l of the functional film 20 from the core 43 is provided. (See FIG. 6) is preferably 0.01 mm or more and 10 mm or less, and more preferably 0.1 mm or more and 3 mm or less.

  In the example shown in FIG. 6, when the mold 40 is clamped, the core 43 of the male mold 41 comes into contact with the base material 13 from the functional film 20 side, and the recess 48 of the female mold 46 is formed. The bottom is in contact with the base material 13 from the side opposite to the functional film 20. Therefore, in the manufactured container 10, the base material 13 comes to form a part of 2nd surface 11b of the bottom part 11. FIG. In other words, the minimum thickness of the bottom 11 of the container 10 is the thickness of the base material 13. In this case, in the in-mold molding using the mold 40, the base material 13 can be more stably held in the cavity cy while effectively preventing the base material 13 from being deformed or damaged. Moreover, the site | part in which the bottom part 11 is formed only with the base material 13 is excellent in light transmittance, Therefore It is suitable for observing the inside of the container 10 from the bottom part 11 with a microscope. However, the present invention is not limited to this example. When the molding die 40 is clamped as in the example shown in FIG. 11 to be referred to later, the core 43 of the male die 41 is formed on the base material 13 from the functional film 20 side. While contacting, the bottom of the recess 48 of the female mold 46 may be separated from the base material 13.

  According to the present embodiment described above, the container 10 includes a bottom portion 11 having a functional film 20 having a function for cells on one surface 11a, and a bottom portion 11 provided on one surface 11a of the bottom portion 11. And a side wall portion 12 that forms an accommodation space S for accommodating cells. One surface 11 a of the bottom portion 11 includes a peripheral region ca covered with the side wall portion 12 and a bottom surface region ba that is surrounded by the peripheral region ca and forms the bottom surface 10 a of the container 10. The functional film 20 is provided across the peripheral area ca and the bottom area ba. That is, in the part where the functional film 20 extends across the peripheral area ca and the bottom area ba, the functional film 20 extends and spreads assuredly to the corner of the bottom surface 10a that becomes the boundary with the inner wall surface 10b. . In particular, when the edge 21 of the functional film 20 is located in the peripheral region ca over the entire circumference, the functional film 20 extends to the corners that form the entire periphery of the bottom surface 10a. Thereby, the function expected by the functional film 20 can be effectively exerted on the cells.

  As an example, when the functional film 20 is a stimulus-responsive polymer film such as a temperature-responsive film, for example, the functional film 20 forming the bottom surface 10a changes its state according to a change in temperature, and the inside of the container 10 Acts on cells. Specifically, the cell sheet cultured on the bottom surface 10a of the container 10 can be separated from the bottom surface 10a by cooling the functional film 20 of the temperature-responsive polymer film. At this time, the cell sheet can be smoothly peeled from the container 10 also at the bottom corner that becomes the boundary between the bottom 10a and the inner wall 10b. Therefore, a delicate and expensive cell sheet can be collected from the container while avoiding damage such as tearing. Since such a cell sheet has a desired shape corresponding to the shape of the container bottom surface 10a, it can be applied to a planned treatment.

  By the way, a plurality of cell sheets produced in the container 10 may be arranged on a site to be treated, for example, on the surface of the heart, such that their edges overlap each other. In such a use, in order to reduce the number of cell sheets used in the treatment site by reducing the overlapping area of two adjacent cell sheets, the outline of the cell sheet is preferably square rather than circular. . Then, in order to produce a square cell sheet, the bottom surface 10a of the container 10 may have a square shape or a shape with chamfered corners as in the example shown in FIG. On the other hand, when the container 10 having such a bottom surface shape is used, the cell sheet is difficult to peel off particularly at the corners, and the possibility of forming a starting point of tearing is increased. Therefore, it can be said that the structure of the container and the method for manufacturing the container described above are particularly suitable for a container whose bottom surface for producing a useful cell sheet has a square shape or a shape with chamfered corners. .

  Further, according to the present embodiment, in manufacturing the container 10, the female mold 46 having the female mold 46 in which the concave portion 48 defining the outer shape of the container 10 is formed and the core 43 disposed in the concave portion 48 is provided. And a male mold 41 for forming a cavity cy for molding the container 10 between the two. The manufacturing method includes a step of filling the cavity cy with a molding material in a state where the base material 13 having the functional film 20 having a function for cells on one side is accommodated in the cavity cy. In this in-mold molding, in the base material 13, a part of the functional film 20 covers the front end surface 44 of the core 43, and at least a part of the edge 21 of the functional film 20 is separated from the front end surface 44 and spreads into the cavity cy. Thus, it is accommodated in the cavity cy. According to such a manufacturing method, in the bottom part 11 of the manufactured container 10, the functional film 20 is formed across the peripheral area ca covered by the side wall part 12 and the bottom surface area ba forming the bottom surface 10 a. The In particular, the functional film 20 covers the entire region of the front end surface 44 of the core 43, and the base member 13 so that the edge 21 of the functional film 20 spreads in the cavity cy apart from the front end surface 44 on the entire circumference. Is accommodated in the cavity cy, the functional film 20 is located in the entire bottom region ba forming the bottom surface 10a of the container 10 at the bottom 11 of the manufactured container 10, and the edge 21 of the functional film 20 is provided. , It is located in the peripheral area ca covered by the side wall 12 over the entire circumference. Therefore, the manufactured container 10 effectively exhibits the function expected of the functional film 20 even at the corner of the bottom surface 10a.

  In this manufacturing method, a part of the functional film 20 covers the front end surface 44 of the core 43 and at least a part of the edge 21 of the functional film 20 extends beyond the edge 44a of the front end surface 44 into the cavity cy. The base material 13 may be accommodated in the cavity cy. Therefore, the positioning of the base material 13 with respect to the male mold 41 does not require high accuracy and can be easily performed. Thereby, the container 10 in which the functional film 20 functions effectively also at the bottom corners can be easily and inexpensively manufactured.

  Furthermore, according to the present embodiment, the manufacturing method accommodates the functional film 20 in the cavity cx of the preforming mold 30 and fills the molding material in the cavity cx of the preforming mold 30 to form the base material 13. The method further includes the step of: That is, the container 10 can be manufactured through two in-mold moldings. According to such a method, the base material 13 having a certain degree of deformation resistance can be manufactured easily and in a short time by the first preforming. In other words, the base material 13 having a certain thickness can be manufactured easily and in a short time. Moreover, according to the base material 13 obtained in this way, the functional film 20 can be easily prevented from being deformed easily during the second molding. As a result, the functional film 20 can be stably extended and spread to the corner of the container bottom surface 10a.

  Various modifications can be made to the above-described embodiment. Hereinafter, an example of modification will be described with reference to the drawings as appropriate. In the following description and the drawings used in the following description, for parts that can be configured in the same manner as the above-described embodiment, the same reference numerals as those used for the corresponding parts in the above-described embodiment are used. A duplicate description is omitted.

  In the above-described embodiment, the example has been described in which the bottom surface 10a of the container 10 has a shape in which a square or a square corner is chamfered in a plan view. A container having a bottom surface 10a having a square shape or a chamfered corner is useful as a container for culturing cells to produce a cell sheet, whereas in a conventional container, the functional membrane is formed on the bottom surface of the container. Problems due to not spreading to the corners could be noticeable. For this reason, the device relating to the container structure and the device manufacturing method described above in which the functional film 20 stably extends and spreads to the corners of the container bottom surface 10a has a shape in which the bottom surface 10a is chamfered with a square or a square corner. This is suitable for the container 10. However, the above-described container configuration and manufacturing method are not limited to a container having a bottom surface that is square or chamfered at a corner portion of the rectangle, but a container having a bottom surface that is a polygon other than a rectangle or a shape whose corners are chamfered. It is also suitable for the container 10 whose bottom surface 10a shown in FIG. 5 is circular, and in these examples, the same effects as the above-described effects resulting from the container configuration and the manufacturing method can be achieved. it can. The container 10 shown in FIG. 8 is different from the container in the embodiment described above in the shape of the bottom surface, and can be otherwise the same as the container in the embodiment described above.

  In the above-described embodiment, an example in which the container 10 has only one storage space S has been described. However, the present invention is not limited to this example. For example, as shown in FIGS. 9 and 10, the container configuration and the manufacturing method described above can be applied to the container 10 having a plurality of storage spaces S. In the example shown in FIGS. 9 and 10, the container 10 has a total of ten storage spaces S. The container 10 also has a bottom part 11 and a side wall part 12. The bottom 11 has a first surface 11a and a second surface 11b that face each other. The first surface 11a has a peripheral region ca where the side wall portion 12 is provided, and a total of ten bottom surface regions ba that form the bottom surface 10a of the accommodation space S. The functional film 20 is formed across the peripheral area ca and the bottom area ba, and forms part of the first surface 11a of the bottom 11. In the illustrated example, a total of ten functional films 20 are provided corresponding to each bottom region ba. The edge 21 of each functional film 20 may be located in the peripheral area ca area surrounding the corresponding bottom area ba over the entire circumference. In the example shown in FIGS. 9 and 10, the same operational effects as the above-described operational effects can be obtained.

  Moreover, the container 10 shown by FIG.9 and FIG.10 may be manufactured by the same method as the manufacturing method mentioned above. Specifically, the container 10 can be manufactured by in-mold molding using the molding die 40 shown in FIG. The molding die 40 includes a female mold 46 in which a recess 48 that defines the outer shape of the container 10 is formed, and a core 43 that is disposed in the recess 48, for molding the container 10 between the female mold 46. And a male mold 41 that forms a cavity cy. This manufacturing method includes a step of filling the cavity cy with a molding material in a state where the base material 13 having the functional film 20 on one surface is accommodated in the cavity cy. As shown in FIG. 11, the base material 13 includes a cavity in which a part of the functional film 20 covers the tip surface 44 of the core 43 and at least a part of the edge 21 of the function film 20 exceeds the edge 44 a of the tip surface 44. It is accommodated in the cavity cy so as to spread in cy. In the molding step shown in FIG. 11, the base material 13 is such that the functional film 20 covers the entire region of the tip surface 44 of the core 43, and the edge portion 21 of the function film 20 is separated from the tip surface 44 on the entire circumference. And you may make it spread in the cavity cy. In the container 10 manufactured by the manufacturing method of FIG. 11, the relationship between the material forming the support 14 of the base material 13 and the material that is injected in the molding process using the molding die 40 and forms the part of the bottom portion 11 and the side wall portion 12. Thus, the interface omitted in FIG. 10 is observed at the bottom 11. Further, the base material 13 may be produced by in-mold molding using the above-described preforming mold 30. This preforming is performed by filling the cavity cx of the preforming mold 30 with a molding material in a state where the functional substrate 15 including the functional film 20 is accommodated in the cavity cx of the preforming mold 30. Also in the example shown in FIG. 11, the same operational effects as the above-described operational effects can be achieved.

  Unlike the manufacturing method shown in FIGS. 9 to 11, the functional film 20 straddles two or more adjacent bottom surface regions ba and a peripheral region ca positioned between the two or more bottom surface regions ba. Alternatively, as shown in FIG. 12, one functional film 20 that extends to all the bottom surface areas ba may be provided. In the manufacturing method shown in FIG. 12, as in the manufacturing method shown in FIG. 6, when the molding die 40 is clamped, the core 43 of the male die 41 is attached to the base material 13 from the functional film 20 side. In addition, the bottom of the recess 48 of the female mold 46 is in contact with the base material 13 from the side opposite to the functional film 20. Therefore, in the manufactured container 10, the base material 13 comes to form a part of 2nd surface 11b of the bottom part 11. FIG. In other words, the minimum thickness of the bottom 11 of the container 10 is the thickness of the base material 13.

  In the above-described embodiment, the example in which the base material 13 is produced by the in-mold molding process using the preforming mold 30 is shown. The present invention is not limited to this example, and the preforming process using the preforming mold 30 is deleted. May be. Since the support layer 16 of the functional base 15 that supports the functional film 20 has sufficient strength, the portion of the functional base 15 that does not face the core 43 is deformed during in-mold molding using the molding die 40. It can be sufficiently suppressed. From the viewpoint of imparting sufficient strength to the functional base 15 and preventing deformation of the functional base 15 during in-mold molding using the molding die 40, the thickness ta of the support layer 16 made of a resin material (see FIG. 4) Is effective to be 0.1 mm or more and 10 mm or less, and more preferably 0.5 mm or more and 5 mm or less.

  In addition, although the some modification with respect to one Embodiment mentioned above was demonstrated above, naturally, it is also possible to apply combining several modifications suitably.

  EXAMPLES Hereinafter, although an Example demonstrates this invention further in detail, this invention is not limited to this.

<< Manufacture of containers >>
As described below, containers according to Example 1, Example 2, Comparative Example 1, and Comparative Example 2 were produced. The shape of the container was the shape shown in FIG. That is, the bottom surface of each container was made into a quadrangular shape, especially a square shape.

  First, functional substrates according to Sample 1 and Sample 2 described below were manufactured.

・ Sample 1
A liquid composition was prepared by dissolving N-isopropylacrylamide in isopropyl alcohol to a final concentration of 40% by weight. A 50 μm thick polystyrene film (available from Asahi Kasei Chemicals) was prepared as a support layer. The said composition was apply | coated using the wire bar to the surface which gave the support layer the corona treatment and was made hydrophilic. The coating amount was 1.4 g / m ² . Then, after drying with hot air at 40 ° C., irradiation with an electron beam causes graft polymerization of N-isopropylacrylamide, immobilizing poly-N-isopropylacrylamide on the surface of the support layer, and providing a support layer and a functional membrane. A functional substrate was prepared. The functional film contained poly-N-isopropylacrylamide so as to function as a temperature-responsive polymer film.

・ Sample 2
A support layer comprising a 50 μm thick polystyrene film (available from Asahi Kasei Chemicals) was prepared. The same composition as Sample 1 was applied to the surface of the support layer that had been subjected to corona treatment to be hydrophilized by the gravure direct method. The coating amount was 1.1 g / cm ² and the conveyance speed was 5 m / min. Then, after passing through a dry hood at 40 ° C. for 2 m and drying, electron beam irradiation is performed to graft polymerize N-isopropylacrylamide, and poly-N-isopropylacrylamide is immobilized on the surface of the support layer. And the functional base | substrate which has a functional film was produced. The functional film contained poly-N-isopropylacrylamide so as to function as a temperature-responsive polymer film.

<Example 1>
The produced sample 1 was cut into a sheet having an appropriate size, and then immersed in water for 4 hours and dried. And the functional base | substrate using the sample 1 was cut out to the predetermined magnitude | size using the hand press machine.

  Next, using the injection molding machine (α-100C, FANUC), the above-described in-mold molding described with reference to FIG. 5 was performed, and a base material having a functional film on one surface was produced. As a molding material, polystyrene pellets (PS Japan SGP10) were used. As molding conditions, the resin temperature was 220 ° C. and the mold temperature was 20 ° C.

  Then, using the injection molding machine ((alpha) -100C, FANUC), the above-mentioned in-mold shaping | molding demonstrated with reference to FIG. 6 was performed, and the container which concerns on Example 1 was produced. As a molding material, polystyrene pellets (PS Japan SGP10) were used. As molding conditions, the resin temperature was 220 ° C. and the mold temperature was 20 ° C. In this molding, the base material is covered so that a part of the functional film of the base material covers the entire surface of the front end surface of the core, and the edge of the functional film spreads into the cavity away from the front end surface on the entire circumference. Housed in a cavity of the mold. In the obtained container 10, the edge part of the functional film was positioned in the peripheral region over the entire circumference.

<Example 2>
A container according to Example 2 was produced in the same manner as in Example 1 except that a functional substrate made of Sample 2 was used.

<Comparative Example 1>
The produced sample 1 was cut into a sheet having an appropriate size, and then immersed in water for 4 hours and dried. And the functional base | substrate using the sample 1 was cut out to the predetermined magnitude | size using the hand press machine. The punching shape of the functional substrate was the same shape as the tip surface of the core of the molding die used in the next molding step.

  Then, in-mold molding was performed using an injection molding machine (α-100C, FANUC), and a container according to Comparative Example 1 was produced. The mold was the same as the mold used in Example 1. The functional substrate was positioned with respect to the mold so that the functional membrane was aligned with the outer contour of the male core tip. Thereafter, the mold was clamped to perform in-mold molding. As a molding material, polystyrene pellets (PS Japan SGP10) were used. As molding conditions, the resin temperature was 220 ° C. and the mold temperature was 20 ° C.

<Comparative example 2>
A container according to Comparative Example 2 was prepared in the same manner as Comparative Example 1 except that the functional substrate made of Sample 2 was used, and the other differences were the same as Comparative Example 1.

<< Use of container >>
Cell sheets were prepared using the containers according to Example 1, Example 2, Comparative Example 1 and Comparative Example 2. Each container was sterilized with ultraviolet rays for a predetermined time in a clean bench, and bovine aortic vascular endothelial cells were adjusted to have a surface cell density of 1 × 10 ⁵ cells / cm ² and seeded in each container. The medium used was DMEM containing 10% FBS (manufactured by Sigma), and the culture was performed in a CO ₂ incubator at 37 ° C. and 5% CO ₂ for 48 hours. Then, each container was stored in an incubator under 20 ° C. and 5% CO 2 conditions. After 20 minutes, the product was removed from the incubator at 20 ° C. Next, the temperature of the functional film was adjusted to change the state from adhesive to non-adhesive.

  In the container according to Example 1 and the container according to Example 2, it was possible to take out the rectangular cell sheet from the container without causing damage such as tearing only by making the functional film non-adhesive.

  In the container according to Comparative Example 1 and the container according to Comparative Example 2, when the functional membrane was made non-adhesive, many portions of the square cell sheet were peeled from the bottom surface of the container. However, even when the functional membrane was changed to non-adhesive, the cell sheet did not peel from the container at the corner of the container bottom. When the cell sheet was peeled from the container, the corners of the cell sheet were torn.

S receiving space ca peripheral region ba bottom region cx cavity cy cavity 10 container 10a bottom surface 10b inner wall surface 10c outer wall surface 11 bottom portion 11a first surface 11b second surface 12 side wall portion 13 base material 13a edge portion 14 support 15 functional substrate 16 Support layer 20 Functional film 21 Edge 30 Preliminary mold 31 First mold 32 Suction hole 36 Second mold 37 Injection hole 38 Recess 40 Mold 41 Male mold 42 Suction hole 43 Core 44 Tip surface 44a Edge 46 Female mold 47 Injection Hole 48 Recess

Claims (3)

In order to mold a container for containing cells between a female mold in which a recess defining an outer shape of the container is formed and a female mold having a core disposed in the recess. A male mold that forms a cavity of
A step of filling the cavity with a molding material in a state where a base material having a functional membrane having a function for cells on one side is accommodated in the cavity;
The base material is formed in the cavity such that a part of the functional film covers the tip surface of the core and at least a part of an edge of the functional film is located in the cavity apart from the tip surface. A method for manufacturing a container to be accommodated.   The base material is such that the functional film covers the entire area of the tip surface of the core, and the edge of the functional film is located in the cavity apart from the tip surface on the entire circumference thereof. The manufacturing method of the container of Claim 1 accommodated in the said cavity.   3. The method according to claim 1, further comprising a step of filling the molding material in the cavity of the preforming die in a state where the functional film is accommodated in the cavity of the preforming die, and molding the base material. Manufacturing method of the container.

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