JP2017165664A - Cleaning agent and method for producing the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a cleaning agent having an excellent foaming property and capable of prompting rapid elution of protease.SOLUTION: According to the present invention, there is provided a cleaning agent consisting of an aggregate of granular materials containing a proteolytic enzyme and having a particle diameter of 0.1 to 2.0 mm. The granular materials have rough surfaces and are amorphous in shape. In addition, when adding 5 parts by weight of water to 1 part by weight of the granular materials, a thickener, which causes a viscosity of 200 to 35000 mPa-s, is added to the granular materials. The granular materials contain 0.001 to 30 parts by weight of an acrylic polymer per 100 parts by weight.SELECTED DRAWING: Figure 1

Description

  The present invention relates to a cleaning material used for cleaning the skin surface such as the face and body, and a method for producing the cleaning material.

  Conventionally, many cleaning materials are provided for cleaning the body such as the face and body. Among them, a detergent containing a protease that is a proteolytic enzyme is widely used as one of the detergents that can be expected to have a high cleaning effect (see, for example, Patent Documents 1 and 2).

  Since the protease contained in the cleaning material can also decompose protein stains deposited on fine irregularities on the skin surface, a high cleaning effect can be obtained.

JP 2002-326924 A JP 2005-192403 A

  By the way, when face washing is assumed as an example of body washing, the time required for face washing is about several tens of seconds, and generally about 1 to 2 minutes at most.

  However, the protease contained in the cleaning material requires a certain amount of time until it fully exhibits the expression of the enzyme activity by the protease.

  However, in spite of this, the conventional cleaning material simply contains protease as a constituent component, and it is difficult to say that the sufficient degradation effect and cleaning effect by the protease can be exhibited during the cleaning time. This is conspicuous in a powdery cleaning material in which water is added and foamed, and it is considered that it takes time to elute the protease in the added water to obtain a sufficient protease concentration.

  On the other hand, when adding water to the powdery cleaning material and foaming, if the cleaning material is made into fine powder, the contact area with water can be expanded, and the protease can be eluted more quickly into the water. Can function.

  In general, when a fine powder is dissolved to prepare an aqueous solution, there is a problem that the powder becomes a paste to form a splice to inhibit complete dissolution of the powder. Specifically, fine powder dissolves from the powder surface to the inside of the powder when water is added, but the powder surface is insufficiently dissolved in water, and the powder surface is highly soluble. A viscous coating layer is formed, the contact between the inside of the powder and water is blocked, and it becomes difficult to completely dissolve. Furthermore, the powder with the coating layer gathered in a paste form to form a so-called pollen, which makes it more difficult for the powder to elute into water.

  In this way, even if the cleaning material is a fine powder, the cleaning material forms a splint and the elution of the cleaning material components is hindered. There arises a problem that the foamability is deteriorated.

  This invention is made | formed in view of such a situation, Comprising: It has the foaming property, and provides the washing | cleaning material which can accelerate | stimulate the elution of protease rapidly. The present invention also provides a method for producing the cleaning material.

  In order to solve the above conventional problems, in the cleaning material according to the present invention, (1) a cleaning material comprising an aggregate of granules having a particle size of 0.1 to 2.0 mm containing a proteolytic enzyme, The granular material is characterized by having an irregular shape with rough surface.

The cleaning material according to the present invention is also characterized by the following points.
(2) When the granular material is mixed by adding 5 parts by weight of water to 1 part by weight of the granular material, a thickener that generates a viscosity of 200 to 35000 mPa · s is added.
(3) The granular material contains 0.001 to 30 parts by weight of an acrylic polymer per 100 parts by weight.
(4) The granular material contains 0.01 to 10 parts by weight per 100 parts by weight of ore powder containing an aluminum compound as a substantial component.
(5) The surface of the granular material is formed in a concavo-convex shape in which substantially spherical small particles having a particle diameter of 0.1 to 500 μm are scattered on the surface of the irregularly shaped base particles.

  In the method for producing a cleaning material according to the present invention, (6) a protease, an amino acid, an ore powder containing an aluminum compound as a substantial constituent, an acrylic polymer, and an excipient are substantially uniform. It also has a mixing step of obtaining a mixed powder by powder mixing, and a granulating step of impregnating the obtained mixed powder with ethanol to obtain an amorphous granule having a particle size of 0.1 to 2.0 mm. Has characteristics.

  According to the cleaning material of the present invention, it is a cleaning material comprising an aggregate of granules having a particle size of 0.1 to 2.0 mm containing a proteolytic enzyme, and the granular material has an irregular surface rough shape. Therefore, it is possible to provide a cleaning material that can promote prompt elution of protease while having excellent foaming properties.

  In addition, when 5 parts by weight of water is added to and mixed with 1 part by weight of the granular body, the granular body is foamed by adding a thickener that generates a viscosity of 200 to 35000 mPa · s. The washed cleaning solution can be brought into close contact with the skin, and the flow rate of bubbles on the skin can be suppressed as much as possible. Therefore, the foam can be further stagnated on the skin, and the decomposition effect by the protease can be efficiently generated.

  Further, if the granular material contains 0.001 to 30 parts by weight of acrylic polymer per 100 parts by weight, the foamed cleaning solution is brought into close contact with the skin, and the flow rate of foam on the skin is increased. It can be suppressed as much as possible. Therefore, the foam can be further stagnated on the skin, and the decomposition effect by the protease can be efficiently generated. And since the acrylic polymer is used, it can be set as the washing | cleaning agent gentle with respect to skin.

  In addition, if the granule contains 0.01 to 10 parts by weight of ore powder containing an aluminum compound as a substantial constituent per 100 parts by weight, the enzyme activity is increased by imparting an optimum environment to the protease. Thus, the degradation effect by the protease described above can be further promoted.

  Further, if the surface of the granular material is formed in an irregular shape in which substantially spherical small particles having a particle diameter of 0.1 to 500 μm are scattered on the surface of the irregularly shaped substrate particles, the contact area with water is increased, and the surface of the particles is increased. While preventing the formation of powder, the dissolution of the granular material can be promoted promptly. Further, the contact of the granular material with the skin becomes gentle due to the small particles, and it is possible to prevent an excessive frictional burden from being applied to the skin during cleaning.

  In addition, if the clay content of the small particles is lower than that of the base particles, the small particles can be dissolved quickly, while the dissolution rate of the base particles is slightly delayed to give a time difference to the dissolution rate. it can. Therefore, in the initial stage of foaming, in a state where the flow of the cleaning solution is high (low viscosity), promptly precede the fine parts of the skin to reach the enzyme, and gently gently wrap the entire skin surface with high-viscosity foam. The enzyme can function while washing, and more effective washing can be performed.

  In addition, according to the method for producing a cleaning material according to the present embodiment, protease, amino acid, ore powder containing an aluminum compound as a substantial constituent, an acrylic polymer, and an excipient are substantially uniform. Because it has a mixing step of obtaining a mixed powder by powder mixing, and a granulating step of impregnating the obtained mixed powder with ethanol to obtain an irregular shaped particle having a particle size of 0.1 to 2.0 mm It is possible to form individual granular materials in the detergent into an irregular shape with rough surface, avoid the formation of spatter while promoting the elution of the detergent ingredients, and have excellent foaming properties However, it is possible to provide a method for producing a cleaning material that can promote rapid elution of protease. In addition, the surface of the irregularly shaped substrate particles is formed in a concavo-convex shape in which substantially spherical small particles having a particle size of 0.1 to 500 μm are scattered, and the small particles have a lower clay concentration than the substrate particles. Can be easily formed.

It is explanatory drawing which showed the microscopic image of the cleaning material which concerns on this embodiment. It is explanatory drawing which shows a verification test result. It is explanatory drawing which shows a verification test result. It is explanatory drawing which shows a verification test result.

  The present invention is a cleaning agent comprising an aggregate of granules having a particle size of 0.1 to 2.0 mm containing a proteolytic enzyme, wherein the granules have an irregular surface rough shape. A cleaning fee is provided. In particular, the cleaning material according to the present embodiment is used for cleaning by taking out a small amount of granular cleaning material from the palm of the hand and adding a small amount of water or hot water to the foam. In the following description, water or hot water used for foaming after dissolving the cleaning material is referred to as “dissolved water”, and a solution in which the cleaning material is dissolved is referred to as “cleaning solution”.

  The proteolytic enzyme used in the cleaning material according to the present embodiment is not particularly limited as long as it can decompose proteins such as aging unnecessary keratin and dirt existing on the skin surface. For example, exoprotease or endoprotease can be employed. . Among these proteolytic enzymes, enzymes classified as endoproteases are preferable. For example, serine proteinases, cysteine proteinases, metalloproteinases, and aspartic proteinases can be employed.

  For example, as serine proteinase, trypsin, Bacillus subtilis origin, Aspergillus origin alkaline proteinase etc. can be mentioned. Examples of cysteine proteinases include bromelain, papain, actinidine, ficin and the like. Examples of metalloproteinases include Bacillus subtilis-derived and Aspergillus-derived neutral proteinases. Examples of aspartic proteinase include rennin, pepsin, Aspergillus-derived acidic proteinase, and the like.

  The proteinase to be employed is not particularly limited, and any combination of these proteinases may be used. The optimum pH of the proteinase is not limited to any one of acidic, neutral, and alkaline. However, as a facial cleanser, for example, a weakly acidic to alkaline or neutral to alkaline pH is optimum. May be adopted. Moreover, it is good also as a prescription except a metalloproteinase.

In the cleaning material according to the present embodiment, the particle size of the granular material is 0.1 to 2.0 mm.
If the particle size of this granule is less than 0.1 mm, it is difficult to form the surface of each granule coarsely because the particle size is too small, and when sprinkling with dissolved water on the palm, Formation is encouraged.

  On the other hand, if the particle size exceeds 2.0 mm, the particle size of each granule is too large. Therefore, when a certain amount of dissolved water is added to the cleaning material, The contact area becomes smaller and the cleaning material is not easily dissolved quickly. Therefore, the components of the cleaning material contained in the granule are difficult to be uniformly dispersed, and the enzyme activity cannot be promoted. By making the particle size 0.1 mm or more and 2.0 mm or less, the detergent should be dissolved in dissolved water quickly while avoiding spalling to uniformly disperse the detergent ingredients and to form granules that promote enzyme activity. Can do.

  In addition, the granular material has an irregular shape with rough surface. Here, the surface roughening is a state in which the surface roughness of the granular material is formed in a concavo-convex shape of about 0.1 to 500 μm.

  By setting it as such a shape, the granular material can form the contact area with air, water, etc. as much as possible compared with the granular material which has not roughened the surface. Therefore, when dissolved water is added to the cleaning material, protease can be rapidly eluted in the dissolved water. In addition, since it is not necessary to reduce the particle size of the granular material, formation of a splice is avoided.

  In addition, when the overall appearance of the granular material is compared, it is an indefinite shape with different individual shapes, like a potato. By making such an indefinite shape, the corners and protrusions derived from the rough surface irregularities of the granular material are more distorted than in the case where the individual granular materials have a uniform shape. It will be formed protruding.

  Therefore, when foaming, irregular corners and protrusions derived from the individual rough surface shape of the granule generate frictional force with the palm, and the granule is easily crushed and dissolved easily. Become. For this reason, a washing | cleaning-agent component can melt | dissolve and disperse | distribute rapidly to dissolution water, and can promote the raise of protease activity.

  Furthermore, if observed from a microscopic viewpoint, by making the granular material indefinite shape, the large and small number of granular materials separated on the palm of the user have a height from the palm surface to the top of each granular material. Each will be different. Therefore, at the initial stage of foaming of the cleaning material, the granular material having a relatively large particle size receives a stronger frictional force, and a large amount of air is taken in due to the level difference of the granular material, which is efficient. It can prompt the foaming.

  Further, the granular material may be added with a thickener that causes a viscosity of 200 to 35000 mPa · s when 5 parts by weight of water is added to 1 part by weight of the granular material and mixed.

  The thickener is not particularly limited as long as the above-mentioned viscosity can be produced at the above-mentioned addition ratio. For example, natural polymers, synthetic polymers, semi-synthetic polymers, inorganic thickeners, these A salt or derivative thereof can be employed.

  For example, for natural polymers, gelatin, casein, collagen, gum arabic, dextrin, pectin, tragacanth gum, xanthan gum, alginic acid, hyaluronic acid and albumin can be employed.

  For synthetic polymers, polyethylene glycol, polyacrylic acid amide, ethylene oxide / propylene oxide copolymer, acrylic acid / maleic acid copolymer, carboxyvinyl polymer and sodium polyacrylate can be employed.

  Moreover, as long as it is a semi-synthetic polymer, propylene glycol alginate, methyl cellulose, carboxymethyl cellulose, carboxymethyl starch, hydroxyethyl cellulose, hydroxypropyl cellulose, cationized cellulose, cyclodextrin and ethyl cellulose can be employed.

  In addition, bentonite, laponite, silica, organically modified bentonite, and hydrophobized silica can be employed as long as they are thickener inorganic substances. Of course, the above-mentioned natural polymer, synthetic polymer, semi-synthetic polymer, and thickener inorganic substance may be used alone or in combination.

  On the other hand, if the viscosity is less than 200 mPa · s, bubbles formed by bubbling of the cleaning solution are liable to break, and there is a problem that the bubbles are less likely to stay on the skin. On the other hand, if it exceeds 35000 mPa · s, the viscosity of the cleaning solution becomes excessively high, and fine bubbles are hardly generated. Further, there arises a problem that the detergent component is difficult to be uniformly dispersed in the solution.

  As mentioned above, by setting the viscosity of the cleaning solution between 200 mPa · s and 35000 mPa · s, the contact area between the cleaning solution and the skin is made as much as possible while quickly raising fine bubbles that are hard to break. Moreover, it is possible to make it close to the skin while suppressing the flow of bubbles. Therefore, such a good-quality cleaning solution can be stagnated on the skin in the form of bubbles, and the decomposition effect by protease can be made efficient.

  Further, the granular material may contain 0.001 to 30 parts by weight of an acrylic polymer per 100 parts by weight.

  The acrylic polymer is not particularly limited as long as it can generate a viscosity of 200 to 35000 mPa · s when 5 parts by weight of water is added to 1 part by weight of the granular material as described above. An acrylic acid / maleic acid copolymer can be employed.

  The molecular weight of the acrylic polymer may be 1 to 10 million, and preferably has a molecular structure with a molecular weight of 3 to 7 million. With such a molecular weight, a viscosity of 200 to 35000 mPa · s can be generated when 5 parts by weight of water is added to 1 part by weight of the granular material and mixed.

  The acrylic polymer may be hydrophilic, hydrophobic, or amphiphilic. Among these, if a hydrophilic acrylic polymer is employed, the adhesion of the cleaning solution to the skin is enhanced, and the user can quickly obtain the protease degradation effect. And it can be set as the washing | cleaning agent of the property which has high safety | security and gives the skin a moisture retention effect.

  By the way, the point which contains an aluminum component is mentioned as one of the characteristic structures of the cleaning material which concerns on this embodiment. This is due to the fact that the present inventors have found that the proteolytic effect by protease is remarkably increased in the presence of a predetermined concentration of aluminum during intensive studies.

  This is considered to be caused by the synergistic action of some of the characteristic configurations of the cleaning material according to the present embodiment, and is useful for rate limiting in a complex system of cleaning solution. It can be said that it is a very interesting result that a substance useful for improving the reaction rate has been found. The inventors imagine that the increase in protease activity in the presence of aluminum is probably due to the fact that a trivalent aluminum ion is folded into a predetermined tertiary structure or has a quaternary structure. It interacts with the hydroxyl group exposed near the outer surface to regulate the behavior of the protease molecule, maintaining the three-dimensional structure of the protease itself, and increasing the activity by inhibiting digestion from other protease molecules. I think that.

  Such a phenomenon can be realized by adding a predetermined concentration of an aluminum compound to the granular material. Specifically, it is good also as containing 0.01-10 weight part of ore powder containing an aluminum compound as a substantial structural component per 100 weight part of granular materials.

  The aluminum compound may be either organic or inorganic. More preferably, it may be an aluminum compound composed of aluminum and silicon in a content ratio of 1: 1 or more, for example, alumina, aluminosilicate or aluminum carbonate, and silicon if silicate or silicon dioxide. A mixture selected from these groups may also be used.

  Here, the substantial content of the aluminum compound contained in the ore powder may be in a state containing 7.0 to 9.0 parts by weight of the aluminum compound per 100 parts by weight of the ore powder.

  Further, when the aluminum compound contained in the cleaning material has a substantial content of 0.0001 parts by weight or more of aluminum per 100 parts by weight of the granular material, the protease activity in the cleaning material is promoted.

  The component of the ore powder is not particularly limited as long as it contains an aluminum compound as a substantial component as described above, but may contain minerals other than the aluminum compound. For example, inorganic metals such as magnesium, zinc, iron, nickel, manganese, chromium, calcium, vanadium, titanium, copper, cerium, potassium, lithium, and sodium may be contained. These metal elements may exist in an ionic state.

  If such an ore powder is adopted, the mineral metal contained in the ore powder functions synergistically in the aluminum compound and the washing solution to rapidly promote protease activity, and without damaging the skin, the mineral component As such, it can have beauty effects such as acne prevention and whitening.

  The ore used as the raw material of the ore powder is not particularly limited, but may be adopted from natural or artificial ore. For example, any one or a mixture of two or more selected from the group consisting of quartzite, wax, dolomite, talc (talc), and clay may be used. If such an ore is used as a raw material, the cleaning solution can adsorb and remove excess sebum and waste products from the skin due to the porous shape of the surface of the ore powder, thereby further enhancing the cleaning effect.

  Further, among the ores, an ore powder made of clay as a raw material is particularly preferable, and may be selected from the group consisting of kaolin, gasur, montmorillonite, white clay, red clay, and a mixture thereof.

  Further, when the content of the ore powder is less than 0.01 parts by weight per 100 parts by weight of the granular material, there arises a problem that the decomposition effect cannot be promoted. On the other hand, even if the amount is 10 parts by weight or more, the content of each ore powder becomes excessive, resulting in a problem that a sufficient decomposition effect by protease cannot be obtained. That is, if 0.01 to 10 parts by weight of ore powder is contained per 100 parts by weight of the granular material, the protease of the cleaning material can cause a necessary and sufficient decomposition reaction while promoting rapid enzyme activity.

  Further, the surface of the granular material may be formed in an uneven shape in which substantially spherical small particles having a particle diameter of 0.1 to 500 μm are scattered on the surface of the irregularly shaped base particles.

  Base particles are composed of a predetermined ratio of cleaning components that can perform cleaning action. The particle size is 0.01 to 1 mm, and the surface of the base particles has multiple irregular shapes and corrugations. You may form in the indefinite shape which has a shape.

  The substantially spherical small particles may be particles having a particle diameter of 0.1 to 500 μm, and may be formed smaller than the base particles.

  A plurality of substantially spherical small particles are scattered on the surface of the substrate particles to form protrusions. The protrusion may be formed by superimposing one or a plurality of small particles on the surface of the base particle and protruding from the surface of the base particle at a height of 0.1 to 1000 μm.

  The small particles may be adhered to the surface of the substrate particles as a part rather than the entire surface, and the surface region where the remaining small particles are not adhered may be used as the substrate particle surface exposed region.

  In addition, since the small particles have a substantially spherical shape, the surface of the base particles and other small particles are attached on a narrow surface. For this reason, when dissolved water is added to the cleaning material, it dissolves from the adhering portion and the small particles are released into the dissolved water, so that the contact area with the dissolved water is increased and the particles are easily dissolved. Moreover, during washing | cleaning, the contact to skin can be made gentle and it can prevent applying an excessive friction burden.

  In addition, in the part where a plurality of small particles are superimposed (stacked) on the surface of the base particle, a gap containing air is formed inside, and the air present in this gap dissolves the small particles. As a result, it is possible to quickly produce fine and hard-to-break bubbles.

  In addition, as dissolution of the granular material into the dissolved water progresses, the large and small base particles of the concavo-convex shape give a difference in height between the palm and the finger and efficiently mix air into the cleaning solution to cause foaming. Promote.

  The clay concentration of the small particles may be lower than the clay concentration of the base particles. If molding is performed with such a concentration difference, small particles with a small amount of clay components can be dissolved quickly, and the enzyme can be reached in advance of fine parts of the skin. On the other hand, since the base particles have a higher clay concentration than the small particles, the dissolution rate in the dissolved water can be slightly delayed to give a time difference to the dissolution rate of the cleaning material.

  In general, when the water is added to the powder in the aggregated state, the spatter is a paste-like form in which the powder on the aggregated surface quickly deprives the moisture at the initial stage of dissolution when the moisture does not spread sufficiently. This paste is formed by mixing with the powder inside the assembly.

  In order to avoid such a state of pollination, in the cleaning material according to the present embodiment, small particles having a low clay concentration are fast-dissolving and base particles having a high clay concentration are slow-dissolving.

  As described above, the surface of the base particle forms a small particle scattering region where small particles are attached and a base particle surface exposed region where no small particles are attached.

  At the time of foaming, the dissolved water comes into contact with the surface-exposed areas of the fast-dissolving small particles and the slow-dissolving base particles, and among the granular materials, the small particles are quickly with a small amount of water compared to the base particles. Dissolve completely in dissolved water.

  Next, the base particle exposed region gradually starts to dissolve in the small particle elution solution, and further, the small particle scattered region exposed by the elution of the small particles gradually dissolves behind the small particle and base particle exposed region. Thus, a cleaning solution in which the cleaning material, which is an aggregate of granular materials, is completely dissolved is obtained.

  In this way, the difference between the specific shape constituting the granular material and the component concentration of each component shape is referred to as stepwise dissolution in the cleaning material as dissolution of small particles, dissolution of exposed regions of base particles, dissolution of dispersed regions of small particles. By giving a time difference, it is possible to prevent the aggregate of granules from rapidly taking away moisture to form a paste, and to increase the speed until the cleaning material is completely dissolved as much as possible.

  Further, in the region where a plurality of small particles are superimposed on the surface of the base particle, the substantially sealed air is mixed into the cleaning solution along with the dissolution of the fast-dissolving small particles. Even in this stage, it is possible to quickly foam fine bubbles that are hard to break.

  Further, the slow-dissolving substrate particles, as described above, can be eluted stepwise in the surface region, so that the substrate particles become more indefinite shape than before the elution, more uneven portions stand out, and water or The surface area with air will be enlarged.

  By adopting the granular material according to the present embodiment, the properties of the base particles are supported as solids, and the uneven shape of the base particles developed with elution gives water and air and a surface area while giving a height difference between the palm and the fingers. The air is mixed more efficiently into the cleaning solution to promote foaming.

  In general, if the clay-containing concentration of the substrate particles is increased to make it slowly soluble, it takes time for natural elution into dissolved water. However, according to the granular material according to the present embodiment, the protease is eluted as quickly as possible in order to reliably dissolve the cleaning material through the stepwise dissolution as described above, and foaming is promoted. The time until complete dissolution can be shortened by avoiding the formation of.

  That is, in the initial stage of foaming, the protease contained in the small particles is diffused as quickly as possible and fine foams can be generated, while in the final stage of foaming, it is stepped from each region of the slow-dissolving substrate particles. Protease is eluted, and effective foaming is promoted by the difference in height of the base particles, so that rapid foaming and protease elution can be realized.

  The present invention also provides a method for producing a cleaning material. The manufacturing method of the cleaning material which concerns on this embodiment has a mixing process and a granulation process.

  The mixing step is a step of mixing components and additives contained in the cleaning material according to the present invention.

  The mixed powder prepared in this step is a powder mixture of at least protease, amino acid, ore powder containing an aluminum compound as a substantial constituent, an acrylic polymer, and an excipient substantially uniformly. It only has to be present, and it may contain components and additives such as surfactants, perfumes, plant extracts, preservatives, feel improvers, moisturizers, and cosmetic ingredients. The content in the mixed powder is not particularly specified, but the components and additives exemplified below can be arbitrarily selected and used in combination.

  The amino acid may have a molecular structure having a molecular weight of 60 to 190. The acid, glutamine, histidine, and amino acid derivatives thereof may be any one selected from these groups or a mixture of two or more.

  As the excipient, for example, corn starch, dextrin, starch and the like can be used.

  Moreover, surfactant can be employ | adopted from anionic surfactant, a cationic surfactant, an amphoteric surfactant, a nonionic surfactant, and fatty acid soap. Of these surfactants, fatty acid soap is preferable, and potassium soap base is particularly preferable.

  The mixing ratio of the raw materials constituting the mixed powder is 0.01 to 2.0 parts by weight of amino acids, preferably 0.04 to 0.16 parts by weight, 0.05 to 5.0 parts by weight of the ore powder, and 0.1 to 10 parts by weight of the acrylic polymer. Is desirable. Moreover, the protease should just contain 10-40000U per 100 weight part of washing | cleaning materials. With such a mixing ratio, a good cleaning effect can be obtained. Moreover, you may add an excipient | filler, surfactant, vitamins, etc. further as needed.

  The granulation step is a step of impregnating the mixed powder obtained in the mixing step with alcohol, for example ethanol, to obtain an irregular shaped granule having a particle size of 0.1 to 2.0 mm.

  Granulation is performed by adding and impregnating 0.1 to 5 parts by weight of ethanol to 100 parts by weight of the mixed powder and stirring. More preferably, by adding 0.1 to 1 part by weight of ethanol to 100 parts by weight of the mixed powder and stirring, an irregularly shaped granule having a particle diameter of 0.1 to 2.0 mm in the granulation step described later Can be obtained. The ethanol may be natural alcohol, synthetic alcohol, hydrous alcohol, or a derivative thereof.

  Thus, by adding the alcohol (ethanol) and granulating it into an indefinite shape, in the cleaning material, the ore powder, the protease and the amino acid containing the aluminum compound as a substantial component are intervened by the alcohol. The clay-protease-alcohol-amino acid complex formed integrally is included.

  Specifically, aluminum is exposed on the clay surface by spraying ethanol on the powder mixture. Further, hydroxyl groups are exposed on the protease surface. Then, the hydroxyl group on the protease surface is coordinated to aluminum on the clay surface in the presence of the alcohol molecule, so that the protease molecule is chelated to the clay surface. That is, the aluminum atom on the clay surface and the hydroxyl group on the protease surface interact with each other via alcohol, and a large number of protease molecules are attached to the clay surface.

  In addition, the surface of the protease molecule other than the surface adhering to the clay (hereinafter also referred to as the non-clay facing surface) interacts with the amino acid by the same alcohol and is protected by the amino acid molecule. As a result, the three-dimensional structure of the protease molecule is more firmly maintained.

  That is, a fine protease molecule is formed on the surface of coarse clay particles by clay having an aluminum atom on the surface, a protease molecule, and an alcohol molecule that promotes a coordinate bond between the aluminum of the clay and the hydroxyl group of the protease. A clay-protease complex is formed in a large number of attached states, and a clay-protease-alcohol-amino acid complex is formed in which the clay non-opposing surface of the protease in the clay-protease complex is protected by an amino acid. Is done.

  In this clay-protease-alcohol-amino acid complex, when dissolving water is added to the detergent and the detergent is foamed and dissolved, the amino acid that protected the protease is released from the complex at a relatively early stage. Leaves the protected state and becomes active (decomposable).

  On the other hand, the amino acid released from the clay-protease-alcohol complex exhibits predetermined effects such as moisturizing, skin softening effect and skin irritation mitigating effect on the user's skin. The alcohol molecules that contribute to the interaction between clay and protease are less likely to leave than the alcohol molecules that contribute to the interaction between protease and amino acid, and the clay molecule is longer than the timing at which the amino acid is released. The protease-alcohol complex is maintained.

  In addition, the clay-protease-alcohol complex is a state in which a large number of fine protease molecules in an active state (deprived of the protected state) are attached to the surface of coarse clay particles. It can be said that the portion is concentrated to a high concentration in a state where the protease is active.

  Therefore, this clay-protease-alcohol complex causes high-concentration protease activity in every corner such as wrinkles and pores of the skin, and exhibits a very effective cleaning effect.

  In addition, the protease is in a state of being restricted by the clay, the predetermined protease molecule can be prevented from being digested by other protease molecules, and the protease titer can be maintained at a high level.

  In this way, the protease is coordinated and bonded to the aluminum present on the surface of the clay via an alcohol, so that the protease molecule is apparently adsorbed on the clay peripheral surface, and the three-dimensional structure retention effect of the protease, An effect of preventing digestion between protease molecules and an effect of preventing aggregation occur.

  From another point of view, it can also be said that this is a technique for improving the cleaning effect as a cleaning material by assembling protease molecules around clay to form a clay-protease-alcohol complex.

Hereinafter, the cleaning material and the method for manufacturing the cleaning material according to the present embodiment will be further described with reference to actual manufacturing processes, experimental results, and the like.
[1. Preparation of cleaning material
In a 5 liter stirring vessel, 50000 U of protease per kg of mixed powder, 10 g of sodium polyacrylate, 10 g of kaolin powder containing aluminum compound, 200 g of potassium mineral base, 0.008 g of amino acids, and the remaining Corn starch was added and prepared so that the total weight was 1 kg, and the mixture was well stirred and mixed with powder to obtain a mixed powder (mixing step).

  Protease 1U here refers to 1 ml of enzyme solution added to 5 ml of 0.6% milk casein (pH 7.5, 0.05M phosphate buffer) and reacted at a temperature of 30 ° C. for 10 minutes. This refers to the activity of an enzyme that produces a non-proteinaceous folin colored product corresponding to 1 μg of tyrosine (protease titer test method).

  Then, about 5 ml of absolute ethanol is sprayed on the mixed powder using a spray, the mixed powder is impregnated with absolute ethanol, and stirred well to form irregular shaped particles having a particle diameter of 0.1 to 2.0 mm. Obtained (granulation step). And the aggregate of the obtained granular material was used as the cleaning material A according to this embodiment.

[2. (Confirmation test)
Next, the prepared cleaning material A was subjected to confirmation tests such as microscopic verification, foaming time verification, and protein resolution verification.

(1. Microscopic observation)
First, the cleaning material A was observed using an optical microscope. As a result, as can be seen from the microscopic image shown in FIG. 1, it was confirmed that the surface of each granular material of the cleaning material A was rough.

  Moreover, it was confirmed that each granular material of the cleaning material A has an indefinite shape having a different size. Further, the particle size of the granular material in the microscopic image of this microscopic observation was about 0.10 mm to 0.30 mm.

  Further, as shown by the white arrow in FIG. 1, it was confirmed that the granular material was formed by scattering substantially spherical small particles in the base particles. It was confirmed that the particle size of the base particles was about 0.08 mm to 0.28 mm, and the size of the small particles was about 3 to 40 μm.

  From the microscopic image shown in FIG. 1A, the surface of the substrate particle has a plurality of irregular shapes and corrugations, and an overhang shape such as “Ω” type, and from the microscopic image shown in FIG. A substrate particle surface exposed region, which is a surface region to which no is adhered, was confirmed.

  Further, from the difference in contrast between the small particles and the base particles, it was confirmed that the content of the clay component in the small particles was lower than the content of the clay component in the base particles.

(2. Verification of foamability)
Next, the solubility of the cleaning material A in dissolved water and the foaming property were verified. In this verification, as a comparative control, measurement was also performed on a cleaning material having a smooth surface (hereinafter referred to as comparative cleaning material X). In addition, the comparative cleaning material X is an amorphous material having a particle size of 1.0 to 4.0 mm in the granulation step in the preparation step of the cleaning material A, and then the granular material is the granular material of the cleaning material A. Then, until the particle size becomes approximately the same particle size (0.1 to 2.0 mm), the aggregate is obtained by slowly chamfering and classifying by grinding.

  In the test, 3 mg of cleaning agent A or comparative cleaning agent X is placed in a 100 ml measuring cylinder, and dissolved water (ultra pure water) is added until the total volume reaches 30 ml. After shaking up and down 20 times, the volume of bubbles generated in the graduated cylinder was measured after being left for 5 minutes.

  The volume of foam generated by cleaning material A and comparative cleaning material X was measured as the height from the bottom of the graduated cylinder to the top surface of the foam, and the same experiment was repeated 5 times, so that each cleaning material was dissolved in dissolved water. The solubility and foaming properties were evaluated. The result is shown in FIG.

  According to the foam test results of FIG. 2, the average height to the top surface of the foam obtained with the comparative cleaning material X was 7.8 cm. Immediately after addition of the solution and during shaking, it was confirmed in the graduated cylinder that a pasty powder-like paste was already formed. As the properties of the foam, a large to small number of non-uniform bubbles rise immediately after shaking, and a large to small number of non-uniform bubbles generated immediately after standing for 5 minutes after shaking gradually disappear. Was observed. Also, after standing for 5 minutes, at the time of measurement, the large bubbles generated immediately after shaking have already disappeared, a layer of small and medium non-uniform bubbles appears on the side of the circumference of the graduated cylinder, and there is a splint inside the solution. It was confirmed that it was precipitated at the bottom of the graduated cylinder as an insoluble residue of the comparative cleaning material X.

  On the other hand, the average height to the top surface of the foam obtained with the cleaning material A according to this embodiment is 12.3 cm, which is about 4 cm higher than the average height to the top surface of the foam obtained with the comparative cleaning material X. It was. A large to small number of bubbles rose with fine bubbles immediately after the addition of the solution, and no splinting was observed at the bottom of the graduated cylinder immediately after shaking. In addition, it can be confirmed that small bubbles and finer bubbles are stagnating inside the measuring cylinder, although large bubbles will partially break the bubbles within 5 minutes of leaving the measuring cylinder after shaking. It was. In particular, it was confirmed that small bubbles and fine bubbles slowly flowed down on the inner surface of the graduated cylinder while the position of the upper surface of the bubble was lowered by defoaming. In the measurement after standing for 5 minutes, small bubbles and fine bubbles appeared on the side surface of the circumference of the graduated cylinder as an orderly layer of bubbles.

  As described above, the cleaning agent A quickly elutes the surfactant into the solution, and has an excellent foaming property as compared with the cleaning agent that is not rough surface, so that extremely fine bubbles can be quickly generated. It was shown to foam.

(3. Verification of protease elution rate and protease activity)
Next, the protease elution rate and protease activity of Cleaning Agent A were verified. In addition, as a further aspect of the cleaning material according to the present embodiment, six cleaning materials B1 to B6 that have different surface presence and presence of thickeners and aluminum components were also examined. In addition, as a comparative sample, a comparative cleaning material X having no surface roughening was also examined. The composition of the cleaning material is shown in Table 1 and Table 2.

  In the test, 0.4 g of cleaning material A or each comparative cleaning material was accommodated in a 15 ml capacity plastic tube, dissolved by adding 3 ml of water and then using a stirrer for a predetermined time, and depending on the stirring time. It was judged whether the elution was quick or not by measuring the activity of the protease. Moreover, the stirring apparatus was preset so that it might become stirring power comparable as foaming with a palm. The result is shown in FIG.

  As can be seen from FIG. 3, the cleaning material A and the cleaning materials B1 to B6 according to the present embodiment have higher enzyme activity in a predetermined time than the comparative cleaning material X, and the protease is faster than the comparative cleaning material X. Elution.

  Further, when focusing on the cleaning materials B1 to B6, the cleaning materials B1 and B4 containing aluminum were found to have higher activity than the cleaning materials B3 and B6 not containing them. In addition, cleaning agents B2 and B5 containing a thickener showed higher activity than cleaning agents B1, B3, B4 and B6 not containing them.

  When focusing attention on the cleaning material A, the enzyme activity extremely higher than those of the cleaning materials B1 to B6 was recognized by containing both aluminum and a thickener. In particular, as can be seen from FIG. 4, when comparing the approximate curves in each graph up to the initial reaction time of 30 seconds, the slope of the graph showing the change in the activity value of the cleaning material A1 is the highest among the cleaning materials B1 to B6. It was about 3 times that of B2, which showed high activity, and it was shown that the protease activity increased in a very short time after adding water to the detergent.

  As described above, according to the cleaning material according to the present embodiment, the cleaning material is a collection of granular materials having a particle diameter of 0.1 to 2.0 mm containing a proteolytic enzyme, and the granular material is Since it has an irregular surface rough shape, it is possible to provide a cleaning material that can promote prompt protease elution while having excellent foaming properties.

  Finally, the description of each embodiment described above is an example of the present invention, and the present invention is not limited to the above-described embodiment. For this reason, it is a matter of course that various modifications can be made in accordance with the design and the like as long as they do not depart from the technical idea according to the present invention other than the embodiments described above.

Claims (7)

A cleaning material comprising an aggregate of granules having a particle size of 0.1 to 2.0 mm containing a proteolytic enzyme,
The granular material has an irregular shape with rough surface, and a cleaning material.   The said granular material added the thickener which raise | generates the viscosity of 200-35000 mPa * s, when adding 5 weight part of water with respect to 1 weight part of the said granular material, and mixing. Item 10. The cleaning material according to Item 1.   The cleaning material according to claim 1 or 2, wherein the granular material contains 0.001 to 30 parts by weight of an acrylic polymer per 100 parts by weight.   The said granular material contains 0.01-10 weight part per 100 weight part of the ore powder which contains an aluminum compound as a substantial structural component, The cleaning material of any one of Claims 1-3 characterized by the above-mentioned.   The surface of the granular material is formed in a concavo-convex shape in which substantially spherical small particles having a particle diameter of 0.1 to 500 μm are scattered on the surface of an irregularly shaped base particle. Cleaning fee as described in   The cleaning material according to claim 5, wherein the small particles have a lower clay concentration than the base particles. A mixing step of obtaining a mixed powder by substantially uniformly mixing powder of an ore powder containing an protease, an amino acid, an aluminum compound as a substantial constituent, an acrylic polymer, and an excipient;
A granulation step of impregnating the obtained mixed powder with ethanol to obtain an irregular granule having a particle size of 0.1 to 2.0 mm;
A method for producing a cleaning material, comprising: