JP2017085928A - Microorganism culture device and microorganism culture method utilizing heat storage agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a microorganism culture device and a microorganism culture method which do not use a heater but utilize hot water as a heat source.SOLUTION: A microorganism culture device utilizing heat storage agent is made up of: a container including a heat insulation material in an internal space and an inner peripheral surface; a heat storage agent which is held in the container and equipped with a groove for housing a culture part which has microorganism culture solution; and a gap which is positioned between the heat insulation material and the heat storage agent and holds hot water. Further, in a microorganism culture method, the heat storage agent having a groove is housed in the container including the heat insulation material in the internal space and the inner peripheral surface, the culture part having microorganism culture solution is positioned at the groove, hot water is pooled in the gap formed between the heat insulation material and the heat storage agent, heat of the hot water is transferred to the culture part via the heat storage agent while the temperature of the water is adjusted to microorganism culture temperature, and a microorganism is cultured in the microorganism culture solution to be heated.SELECTED DRAWING: Figure 1

Description

  The present invention relates to a microorganism culturing apparatus and a microorganism culturing method that use hot water as a heat source without using a heater. More specifically, the present invention relates to a microorganism culturing apparatus and a microorganism culturing method that use hot water and a heat storage agent and are excellent in portability mainly suitable for test tube culture, simple, stable and capable of long-term culture.

  Conventionally, an object to be inspected collected from an object to be inspected for microorganisms is inoculated into a culture solution in a test tube and cultured with a large culture apparatus to check for the presence of microorganisms. On the other hand, portable culture apparatuses that cultivate a test tube standing in an incubator in which a heater is embedded have also become widespread.

  Furthermore, the invention using a heater and a heat storage material member has also been developed (Patent Document 1). Patent Document 1 discloses a test bottle heating block, a culture apparatus and a culture method using the test bottle heating block, which can keep the temperature in the test bottle constant even when the indoor temperature fluctuates. The heat insulating member 6 is provided at least on the side facing the heating means 3 of the metal heat storage member 5 having the support hole 12 for inserting the inspection bottle 11. Note that a heater is used as the heating means.

  However, an apparatus using a heater is an expensive apparatus because it requires an electric circuit such as temperature control and a safety device. Moreover, in invention of patent document 1, a heat insulating material intervenes between a heating means and a heat storage member, and heat conduction efficiency is low.

  On the other hand, an invention using a heat storage material without using a heater has also been developed (Patent Document 2). Patent Document 2 is a temperature adjustment member that avoids a decrease in heat release efficiency and a decrease in buffer capacity due to heat distribution non-uniformity inside the heat storage material, and stirring that does not substantially react with the heat storage material 82 inside the heat storage material 82. One or a plurality of tools 83 are preliminarily set in the heat storage material 82, and the heat storage material 82 is used by using a stirring flow generated by changing the relative position of the stirring tool 83 inside the heat storage material 82 during transportation. Eliminates internal thermal non-uniformity. In addition, as the heat storage material 82, paraffin that causes a phase transition from a liquid to a solid at a constant temperature is used.

  However, in the invention of Patent Document 2, since the heat storage material is heated in advance with a thermostatic device, it is simple and portable.

Japanese Patent Laid-Open No. 06-261739 JP 2009-73513 A

  Therefore, the present invention provides a microorganism culture apparatus and a microorganism culture method that use hot water as a heat source without using a heater, and more specifically, portableness that is suitable mainly for test tube culture using hot water and a heat storage agent. An object of the present invention is to provide a microorganism culture apparatus and a microorganism culture method that are excellent, simple, stable and capable of long-term culture.

In order to solve the above problems, the present invention
(1)
An internal space, a container provided with a heat insulating material on the inner peripheral surface, and
A heat storage agent provided with a groove for holding a culture part having a microorganism culture solution, which is held in the container;
Located between the heat insulating material and the heat storage agent, a gap for holding hot water,
A microorganism culturing apparatus using a heat storage agent characterized by comprising:
(2)
An internal space, a container provided with a heat insulating material on the inner peripheral surface, and
A culture part held in the container and having a microorganism culture solution;
A heat storage agent comprising a groove for accommodating the culture part;
Located between the heat insulating material and the heat storage agent, a gap for holding hot water,
A microorganism culturing apparatus using a heat storage agent characterized by comprising:
(3)
The heat storage agent is a substance that melts from solid to liquid with heat,
The melting temperature is a microorganism culture temperature;
The microorganism-cultivating apparatus using the heat storage agent according to (1) or (2), wherein the warm water is warm water higher than the melting temperature.
(4)
The heat storage agent,
A plurality of heat storage agent layers with different melting temperatures,
And,
The melting point of the heat storage agent layer on the warm water side is higher than the melting point of the heat storage agent layer on the culture part side,
The microorganism-culturing apparatus using the heat storage agent according to (3), wherein a melting point of the heat storage agent layer on the culture part side is a microorganism culture temperature.
(5)
The microorganism-cultivating apparatus using the heat storage agent according to any one of (1) to (4), wherein the substance to be melted is oil or fat.
(6)
The said container is provided with a lid | cover, The microorganisms culture apparatus using the thermal storage agent in any one of (1)-(5) characterized by the above-mentioned.
(7)
In a container equipped with an insulating material on the inner space and inner peripheral surface,
Stores heat storage agent with grooves,
Positioning a culture part having a microorganism culture solution in the groove,
Hot water is accumulated in a gap formed between the heat insulating material and the heat storage agent,
The heat of the warm water is adjusted to the microorganism culture temperature via the heat storage agent and transmitted to the culture unit,
A microorganism culturing method using a heat storage agent, wherein microorganisms are cultured in the heated microorganism culture solution.
It was.

  Since the present invention has the above-mentioned configuration, it provides a portable microorganism culture apparatus and a microorganism cultivation method that are superior in portability mainly suitable for test tube culture, using hot water as a heat source without using a heater. Can do. By making the heat storage agent layers into two or more layers and changing the melting point of each heat storage agent, even if hot hot water is roughly used, the temperature of the microorganism culture solution is prevented from rising, and more heat energy is provided Therefore, long-term heat insulation is possible.

1 is a schematic perspective view of a microorganism culturing apparatus using a heat storage agent according to a first embodiment of the present invention. It is a plane schematic diagram of the microorganism culture apparatus using the thermal storage agent which is 2nd embodiment of this invention. It is a plane schematic diagram of the microorganism culture apparatus using the thermal storage agent which is 3rd embodiment of this invention. It is a plane schematic diagram of the microorganism culture apparatus using the thermal storage agent which is 4th embodiment of this invention.

  Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings. However, the present invention is not limited to these embodiments.

  As shown in FIG. 1, a microorganism culture apparatus 1 using a heat storage agent according to the present invention includes a container 2 having a heat insulating material 3 on an inner space and an inner peripheral surface, and a microorganism culture solution 6 a that is held in the container 2. It comprises a heat storage agent 5 having a groove 5b for accommodating and standing the culture unit 6 and a gap 4 which is located between the heat insulating material 3 and the heat storage agent 5 and holds the hot water 7.

  As the heat insulating material 3, those conventionally known and used such as foamed polystyrene having a heat insulating function can be used. The heat insulating material 3 and the heat storage agent 5 may be fixed to the container 2 or detachable. Since the present invention does not require a heater and it is not necessary to preheat the heat storage agent, it can be fixed to the container 2.

  The heat storage agent 5 is preferably a material that melts from a solid to a liquid by heat, such as fats and oils, and the melting temperature may be set to, for example, 30 ° C., 35 ° C., 40 ° C., etc. The temperature of the oil / fat may be controlled by a conventionally known melting point temperature such as changing the composition or hydrogenation. Since heat of fusion is required when the solid melts, the temperature of the heat storage agent 5 is kept constant until all the solid melts. If the maintained temperature is set to the optimum culture temperature for microorganisms, microorganism culture becomes possible. If the heat storage agent 5 has a melting temperature from an individual to a liquid or an evaporation temperature from a liquid to a gas in a target temperature range, that is, if a specific temperature range is maintained during a state change of a substance, It is not limited.

  The warm water 7 may be warm water 7 higher than the melting temperature, and does not require accurate temperature control. Since the melting temperature is within the culture temperature range of the microorganism, the heat storage agent 5 is held in that temperature range. Of course, if a large amount of hot water is brought into contact with the heat storage agent 5, that is, if a large amount of heat exceeding the set melting temperature range is given, the heat storage agent 5 will eventually exceed the culture temperature range. Therefore, the hot water temperature and the amount of water are set in advance so that the amount of heat for maintaining the set temperature range is obtained. For this purpose, the capacity of the gap 4 and the allowable temperature range of the hot water to be stored may be set according to the optimum growth temperature of the cultured microorganism species. Extremely easily, that is, microbial culture is possible.

  Further, a detachable or openable lid 8 may be provided on the top of the container 2. By having the lid 8 provided with the heat insulating material on the inner surface, the heat retaining property is enhanced, and even when the amount of the hot water 7 is small and the temperature is low, the culture can be performed for a long time, and the container 2 can be downsized.

  The culture part 6 can be easily cultured on the spot by using the microorganism culture apparatus 1 using the heat storage agent of the present invention in a test tube or the like. The groove 5b for accommodating and positioning the culture part 6 of the heat storage agent 5 is not limited to one shown in FIG. As the container shape, any shape other than a cylinder, such as a box shape, can be adopted.

  As shown in FIG. 2, the microorganism culture apparatus 1 a using the heat storage agent according to the present invention includes a container 2 a having a heat insulating material 3 a on an inner space and an inner peripheral surface, a container 2 a, and a microorganism culture solution 6 a. The heat storage agent 5a provided with the groove 5b for accommodating the culture unit 6 and the heat insulating material 3 and the heat storage agent 5 are provided, and the gap 4a for holding the hot water 7 is formed. In this embodiment, the hot water 7 is in contact with only one side surface of the heat storage agent 5a. Further, the heat storage agent 5a is provided with a plurality of grooves 5b for accommodating and standing a plurality of culture sections 6 respectively. The container 2a may be provided with a lid.

  As shown in FIG. 3, the microorganism culturing apparatus 1 b using the heat storage agent according to the present invention includes the heat storage agent 5 in Example 1, the first heat storage agent 5 c and the second heat storage agent 5 d. Changed to Moreover, about melting | fusing point of an internal heat storage agent, melting | fusing point of the 1st heat storage agent 5c by the side of the warm water 7 is set higher than melting | fusing point of the 2nd heat storage agent 5d by the side of the culture part 6. FIG.

  For example, the first heat storage agent 5c is an oil having a melting point of 50-60 ° C., and the second heat storage agent 5d is an oil having a melting point near 37 ° C. If such a melting point is set, for example, the temperature of the hot water 7 is roughly set to about 90 ° C., and the first heat storage agent 5c layer serves as a temperature buffer, and prevents contact with the high temperature of the microorganism culture solution 6a. Then, heat is transmitted to the second heat storage agent 5d, and the microorganism culture solution 6a is maintained at a target temperature of 37 ° C.

  That is, the heat is transferred from the hot water 7 to the first heat storage agent 5c first to maintain a stable temperature near the melting point, and then the second heat storage agent 5d has a temperature range suitable for microbial culture, for example, 37 ° C. Secure. As described above, if the heat storage agents have different melting points in a plurality of layers, the heating temperature of the microorganism culture solution 6a can be stabilized and the culture temperature can be maintained for a long time.

  As shown in FIG. 4, the microorganism culture apparatus 1c using the heat storage agent according to the present invention has a higher melting point than the heat storage agent 5a in the hot water 7 side space so as to contact the heat storage agent 5a of Example 2. The first heat storage agent 5e is added. Moreover, about melting | fusing point of an internal heat storage agent, melting | fusing point of the 1st heat storage agent 5e by the side of the warm water 7 is set higher than melting | fusing point of the (1st) heat storage agent 5a by the side of the culture | cultivation part 6.

  As in Example 3, by providing a plurality of heat storage agent layers having different melting points, the heating temperature of the microorganism culture solution 6a can be stabilized and the culture temperature can be maintained for a long time.

  A microorganism culturing method using a microorganism culturing apparatus using a heat agent such as Example 1-4 is performed according to the following procedure.

  The microorganism culturing method using the heat storage agent according to the present invention is a microorganism in which a heat storage agent having a groove 5b is housed in a container provided with a heat insulating material in an inner space and an inner peripheral surface, and the collected sample is suspended in the groove 5b. A culture part having a culture solution is positioned, hot water 7 is stored in a gap formed between the heat insulating material and the heat storage agent, and the heat of the hot water 7 is adjusted to the microorganism culture temperature via the heat storage agent, and the culture part The microorganisms are cultured, for example, overnight to overnight in the microorganism culture solution that is transmitted to 6 and heated. The presence / absence of culturing of the microorganism may be determined by the color of the indicator added to the medium, which has been used conventionally.

DESCRIPTION OF SYMBOLS 1 Microbial culture apparatus 1a using a heat storage agent Microbial culture apparatus 1b using a heat storage agent Microbial culture apparatus 1c using a heat storage agent Microbial culture apparatus 2 using a heat storage agent 2 Container 2a Container 3 Insulating material 3a 5 thermal storage agent 5a thermal storage agent 5b groove 5c first thermal storage agent 5d second thermal storage agent 5e first thermal storage agent 6 culture section 6a microbial culture solution 7 warm water 8 lid

Claims (7)

An internal space, a container provided with a heat insulating material on the inner peripheral surface, and
A heat storage agent provided with a groove for holding a culture part having a microorganism culture solution, which is held in the container;
Located between the heat insulating material and the heat storage agent, a gap for holding hot water,
A microorganism culturing apparatus using a heat storage agent characterized by comprising: An internal space, a container provided with a heat insulating material on the inner peripheral surface, and
A culture part held in the container and having a microorganism culture solution;
A heat storage agent comprising a groove for accommodating the culture part;
Located between the heat insulating material and the heat storage agent, a gap for holding hot water,
A microorganism culturing apparatus using a heat storage agent characterized by comprising: The heat storage agent is a substance that melts from solid to liquid with heat,
The melting temperature is a microorganism culture temperature;
The microbial culture apparatus using a heat storage agent according to claim 1 or 2, wherein the warm water is warm water higher than the melting temperature. The heat storage agent,
A plurality of heat storage agent layers with different melting temperatures,
And,
The melting point of the heat storage agent layer on the warm water side is higher than the melting point of the heat storage agent layer on the culture part side,
4. The microorganism culture apparatus using a heat storage agent according to claim 3, wherein the melting point of the heat storage agent layer on the culture part side is a microorganism culture temperature. The microorganism-cultivating apparatus using a heat storage agent according to any one of claims 1 to 4, wherein the substance to be melted is oil or fat. The microbial culture apparatus using the heat storage agent according to any one of claims 1 to 5, wherein the container includes a lid. In a container equipped with an insulating material on the inner space and inner peripheral surface,
Stores heat storage agent with grooves,
Positioning a culture part having a microorganism culture solution in the groove,
Hot water is accumulated in a gap formed between the heat insulating material and the heat storage agent,
The heat of the warm water is adjusted to the microorganism culture temperature via the heat storage agent and transmitted to the culture unit,
A microorganism culturing method using a heat storage agent, wherein microorganisms are cultured in the heated microorganism culture solution.

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