JP2017067605A - Specimen measurement device and specimen measurement method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a specimen measurement device and a specimen measurement method with which it is possible to reliably measure the state of a specimen without using a filter.SOLUTION: A support member 2 supports a container having at least one first well in which a standard substance is stored and a plurality of second wells in which a measurement object is stored. A photographing unit 4 photographs the image of the whole of the container as a color image. An input unit 3a accepts the concentration of the standard substance of the first well as numerical data. A processing unit 7 generates a calibration curve on the basis of numerical data corresponding to the color and luminosity of the standard substance included in the color image data and the numerical data of the concentration of the standard substance inputted from the input unit, and calculates the concentration of the measurement object included in the color image data as numerical data.SELECTED DRAWING: Figure 2

Description

  The present invention relates to a sample measuring apparatus and a sample measuring method applied to, for example, measurement of a change in the state of a sample.

  This type of sample measurement apparatus performs absorbance measurement using, for example, a CCD camera or a photodiode. That is, a sample is accommodated in each well of a microplate having a plurality of wells, light is irradiated to the sample in each well, and the absorbance of the light transmitted through the sample is detected by a CCD camera or photodiode through a filter. (For example, refer to Patent Documents 1 and 2).

JP-A-10-170519 JP-A-10-170429

  The above-described sample measurement apparatus for measuring absorbance requires a filter for selecting light of a color necessary for measurement from light from the sample. For this reason, it is necessary to replace the filter according to the measurement.

  The present invention has been made based on the above circumstances, and provides a sample measuring apparatus and a sample measuring method capable of reliably measuring the state of a sample without using a filter.

  The sample measurement apparatus of the present invention includes a support that supports a container having at least one first well in which a standard substance is accommodated, a plurality of second wells in which a measurement object is accommodated, and the first, An imaging unit that images the entire surface of the container including the second well and outputs it as color image data, an input unit that inputs the concentration of the standard substance in the first well as numerical data, and a supply from the imaging unit A calibration curve is generated based on numerical data corresponding to the color information and brightness information of the standard substance contained in the color image data and the numerical data of the concentration of the standard substance input from the input unit And a calculation processing unit including a second process for calculating the concentration of the measurement object included in the color image data as numerical data based on the calibration curve.

  The sample measurement apparatus of the present invention includes a support that supports a container having a plurality of wells in which a sample is accommodated, an imaging unit that images the entire surface of the container including the plurality of wells, and outputs the image as color image data. An arithmetic processing unit that calculates numerical data corresponding to the color information and brightness information of the specimen included in the color image data supplied from the imaging unit.

  In the sample measurement method of the present invention, a support member supports a container having at least one first well in which a standard substance is accommodated and a plurality of second wells in which a measurement object is accommodated, and the imaging unit The entire surface of the container including the first and second wells is photographed and output as color image data, and the concentration of the standard substance contained in the at least one first well is numerical data by an input unit. And the numerical value data corresponding to the color information of the standard substance and the brightness information included in the color image data supplied from the imaging unit, and the numerical data corresponding to the brightness information are input from the input unit by the arithmetic processing unit. A calibration curve is generated based on the numerical data of the concentration of the standard substance, and the concentration of the measurement object included in the color image data is calculated as numerical data based on the calibration curve.

  The sample measurement method of the present invention supports a container having a plurality of wells in which a sample is accommodated by a support, images the entire surface of the container including the plurality of wells by an imaging unit, and outputs the image as color image data Then, the arithmetic processing unit calculates numerical data corresponding to the color information and brightness information of the specimen included in the color image data supplied from the imaging unit.

  ADVANTAGE OF THE INVENTION According to this invention, the sample measuring device and sample measuring method which can measure the state of a sample reliably without using a filter can be provided.

The perspective view which shows an example of the external appearance of the sample measuring device which concerns on this embodiment. The top view which shows an example of the display screen of the display part shown in FIG. The block diagram which shows an example of the inside of the sample measuring device which concerns on this embodiment. The top view which shows an example of the microplate applied to this embodiment. 6 is a flowchart showing an example of the operation of the sample measurement apparatus according to the embodiment. The figure which shows an example of the registration screen of the reference material of the display part shown in FIG. The figure which shows an example of the calibration curve corresponding to a reference material. The figure which shows an example of the density | concentration calculated based on the calibration curve shown in FIG.

  Embodiments of the present invention will be described below with reference to the drawings.

(Configuration of sample measuring device)
As shown in FIG. 1, the sample measurement apparatus 1 according to the present embodiment includes a housing 11, a plate setting unit 2, a keyboard 3 a, a mouse 3 b, and a display unit 8. The plate setting unit 2 is provided so as to be freely inserted and removed from the housing 11. The plate setting unit 2 as a support has a tray (not shown). This tray supports, for example, the periphery of the back surface of the microplate 12 as a sample container. In a state where the microplate 12 is set on the tray, the microplate 12 is accommodated in the housing 11.

  The keyboard 3a is used as an input unit for inputting various kinds of information, and the mouse 3b is used for pointing information displayed on the display unit 8.

  The display unit 8 displays various information such as various operation buttons for operating the sample measurement apparatus 1.

  FIG. 2 shows an example of a main screen displayed on the display unit 8. The main screen has a first display unit 8a, a second display unit 8b, and a third display unit 8c.

  The first display portion 8a displays a color image of the microplate 12, identification data of the microplate, and an ELISA (Enzyme-linked immuno-sorbent assay) kit name to be described later.

  The second display unit 8b can be switched by a plurality of tabs. For example, in the calibration curve tab, a calibration curve described later calculated from the numerical data of the concentration of the standard substance of the selected kit and the numerical data of the standard substance included in the displayed color image is displayed.

  In the case of the density tab, there are option marks of “detection target”, “RGB”, “Red”, “Green”, and “Blue”, and display corresponding to the option selected from these marks is possible.

  For example, when “inspection object” is selected, for example, the concentration of the measurement object calculated based on the calibration curve is displayed as numerical data.

  When “RGB” is selected, numerical data obtained by weighting the data of “Red”, “Green”, and “Blue” to obtain an average value is displayed.

  When any one of “Red”, “Green”, and “Blue” is selected, single-color numerical data of red, green, or blue is displayed.

  In addition to a plurality of “setting” buttons, for example, a “live view” button, a “save” button, an “ELISA” button, a “shoot” button, a “print” button, a “register” button, An “image search” button is displayed.

  The plurality of “setting” buttons are buttons for setting various operations of the sample measuring apparatus 1, for example, changing the brightness of the light source described later, changing the brightness of the screen of the display unit 8, When enlarging / reducing a photographed color image is specified, or when processing color image data in RGB, which will be described later, the HSV (Hue: Hue, Saturation: Saturation, Value: Lightness) color space or HSL (Hue, It is possible to set when processing in one of Saturation, Lightness (lightness) color space, HSB (Hue, Saturation, Brightness) color space, or HLS (Hue, Lightness / Luminance) color space. It is said that.

  The “live view” button is a button for instructing display of a raw image being shot.

  The “save” button is a button for instructing to save a photographed unsaved image.

  The “print” button is a button for instructing printing of data such as an image.

  The “ELISA” button is a button for inputting an ELISA kit name and a standard value, and when this button is clicked, the screen is switched to a registration screen.

  The “shoot” button is a button for shooting an image displayed on the first display unit 8a in a live view state.

  The “print” button is a button for instructing printing of various data.

  The “Register” button is a button operated to register an inspector or the like, and when this button is operated, the screen is switched to a registration screen.

  The “image search” button is a button for searching for a recorded image. When this button is operated, the screen is switched to a search screen, and the recorded image can be searched using, for example, a kit name. ing.

  FIG. 3 shows the internal configuration of the sample measuring apparatus 1. Light sources 31, 32, and 33 are disposed in the vicinity of the plate setting unit 2 on which the microplate 12 is set. The light sources 31, 32, 33 are preferably white flat type light sources, for example, in order to keep the luminance uniform with respect to the microplate 12, and are configured by, for example, electroluminescence or light emitting diodes.

  The light source 31 irradiates light to the microplate 12 from the back side of the microplate 12.

  The light source 32 irradiates the microplate 12 with light from above the left side of the microplate 12 (one along the length direction of the microplate 12).

  The light source 33 irradiates the microplate 12 with light from above the right side of the microplate 12 (the other along the length direction of the microplate 12).

  The light sources 31 to 33 are connected to the processing unit 7, and the brightness and the light source to be used can be changed by operating the “setting” button of the display unit 8.

  For example, the imaging unit 4 is disposed above the microplate 12. The imaging unit 4 is mounted on a slider, and the distance between the microplate 12 and the imaging unit 4 can be adjusted. The slider 5 is connected to the processing unit 7 and can be moved by operating the “setting” button on the display unit 8.

  The imaging unit 4 images the microplate 12 from the upper surface. The photographing unit 4 is configured by, for example, a CMOS sensor capable of photographing a color image, and photographs the entire surface of the microplate 12 having a plurality of wells as a color image. Specifically, the imaging unit 4 is irradiated from the light source 31 and transmitted through the microplate 12 and the sample in the well, and is irradiated from the light sources 32 and 33 and reflected from the microplate 12 and the sample in the well. At least one of the received light is received, and RGB color image data is generated. The color image data generated by the photographing unit 4 is sent to the processing unit 7.

  The photographing unit 4 photographed the entire surface of the microplate 12 from above the microplate 12, but is not limited to this. For example, an optical system such as a mirror can be used to reduce the size of the apparatus, It is also possible to photograph the entire surface of the microplate 12 from below the plate 12.

  The barcode reader 6 reads a barcode as identification data (not shown) attached to, for example, the side surface of the microplate 12, and sends identification data corresponding to the read barcode to the processing unit 7.

  Instead of the barcode, other identification data, for example, a QR code (registered trademark) may be attached to the microplate 12, and the identification data may be read by a dedicated reader in place of the barcode reader 6.

  Further, instead of the barcode reader 6, for example, identification data for identifying the microplate 12 may be supplied to the processing unit 7 by an input device such as a keyboard 3a or a mouse 3b.

  Alternatively, a configuration in which identification data such as a barcode is provided on the upper surface of the microplate 12 and the imaging unit 4 recognizes the identification data from the photographed color image data by the processing unit 7 may be adopted.

  The processing unit 7 controls the entire sample measuring device 1 according to various operation commands supplied from the display unit 8.

  As described above, the processing unit 7 controls the light emission of the light sources 31, 32, and 33 so that the specimen on the microplate 12 can be imaged with high accuracy.

  As described above, the processing unit 7 controls the slider 5 and moves the position of the photographing unit 4 so that an image of the entire surface of the microplate 12 can be photographed. Specifically, the size of the microplate 12 varies depending on the number of wells. Therefore, the position of the photographing unit 4 is controlled by the slider 5 so that an image of the entire surface of the microplate 12 can be taken.

  The processing unit 7 processes data based on the RGB data constituting the color image data supplied from the photographing unit 4. The processing unit 7 can perform processing based on the RGB data itself and processing based on any of the HSV color space, the HSB color space, the HSL color space, and the HLS color space. For example, in the case of processing based on the RGB data itself, the color of the specimen in each well and one of the luminous intensity and the luminance is calculated as numerical data from the RGB data.

  In addition, the processing unit 7 calculates a calibration curve based on one of the color of the standard substance, the luminous intensity and the luminance included in the measurement data, and the numerical data of the concentration of the standard substance input from the display unit 8 and the keyboard 3a. . Further, the processing unit 7 calculates the concentration of the measurement object as numerical data from the color image data of the measurement object based on the calculated calibration curve.

  In the case of processing based on any one of the HSV color space, HSB color space, HSL color space, and HLS color space, the processing unit 7 determines the hue, saturation, and brightness of the specimen in each well based on the RGB data. One of the luminance values is calculated as numerical data, and the numerical data of the hue, saturation, brightness, or luminance of the standard substance included in the measurement data, and the concentration of the standard substance input from the display unit 8 or the keyboard 3a. Based on this, a calibration curve is calculated. Specifically, for example, a calibration curve is calculated on the basis of numerical data of brightness or luminance as brightness information and the concentration of the standard substance. Further, the processing unit 7 calculates the concentration of the measurement object as numerical data from the color image data of the measurement object based on the calculated calibration curve.

  The processing unit 7 associates the color image data supplied from the imaging unit 4 with the identification data (including the ELISA kit name) and sends it to the display unit 8 to display the identification data, the ELISA kit name and the color image data. Are stored in the storage unit 9 in association with each other.

  The output unit 10 outputs image data of the microplate 12, identification data of the microplate 12, inspection data, and the like to the server 13 in accordance with instructions from the server 13. For example, when the output unit 10 receives an instruction to read out inspection data from the server 13 and the identification data of the microplate 12 to be read out, the output unit 10 reads out inspection data corresponding to the identification data from the storage unit 9 and stores the inspection data in the server Send to 13. The output unit 10 can be omitted as necessary.

(About ELISA kit)
Next, a case where the sample measurement apparatus 1 of the present embodiment is applied to, for example, ELISA will be described. ELISA is a method for quantifying the amount of antigen or antibody in a sample solution using an antibody or antigen labeled with an enzyme. By using ELISA, various diseases such as collagen disease and rheumatism, bacteria, etc. And virus infection, residual pesticides, etc. can be measured. As the measurement method, there are a direct method, an indirect method, a competitive method, and a sandwich method, and this sample measurement apparatus 1 is compatible with all measurement methods.

  FIG. 4 shows an example of an ELISA kit displayed on the display unit 8. For this reason, numerals 1 to 12 are displayed along the row direction of the microplate 12, and alphabets A to H are displayed along the column direction. Further, a circular display is shown corresponding to these numbers and alphabets.

  As shown in FIG. 4, in the ELISA kit applied to this embodiment, the microplate 12 is a standard microplate having 96 wells. The microplate 12 is not limited to this, and a microplate having more than 96 wells or a microplate having a small number of wells can be applied.

  For example, at least one of the wells (first wells) in the first to third rows of the microplate 12 constitutes a standard part 12a that accommodates a standard substance that is a reference for measurement. In the case of the present embodiment, for example, the wells in the first row and the second row constitute the standard part 12a, and the third to twelfth rows constitute the measurement part 12b that accommodates the sample to be measured. The circular display corresponding to the numbers 1 and 2 indicates that the display state of the other circular display is changed and the wells in the first and second columns are the standard substances.

  In the case of the sandwich method of ELISA, for example, the standard substance antigen (or antibody) added to the wells of rows A to H in the first and second columns of the 12 columns of the microplate on which the antibody (or antigen) is immobilized. ) Are different from each other, and the concentration of the enzyme-labeled secondary antibody (or enzyme label) is made constant. Under these conditions, after the reaction of the standard substance by the antigen / antibody reaction, color is developed at different concentrations when a chromogenic substrate is added. There are also methods using a fluorescent substrate or a luminescent substrate instead of the chromogenic substrate.

  The objects to be measured are accommodated in the wells in the third column to the twelfth column. After the reaction by the antigen / antibody reaction, for example, when a chromogenic substrate is inserted, the color is developed according to the concentration of the substance to be measured in the wells.

  In addition, although the standard part 12a was set to the column, you may set not only to this but to a line.

  The present embodiment does not measure the absorbance of the specimen, but rather information on the color of the specimen (in the case of RGB, color, hue in the case of HSV, etc.) and brightness information (in the case of RGB, one of luminous intensity and luminance) In the case of HSV, etc., saturation and one of brightness and luminance) are measured based on RGB color image data of the microplate taken. Note that the saturation may be handled as color information.

  The reason why the first row and the second row of wells are used as the standard portion 12a is that if only one row is used as the standard portion 12a, the measurement accuracy may be lowered due to variations in color image data of the standard substance. Because there is. Depending on the kit used, 3 rows instead of 2 rows may be used as the standard part.

  When two to three rows are used as the standard portion 12a, the average value of the color image data of the two rows of standard substances or the average value of the color image data of the three rows of standard substances is obtained, and this average value is used as a reference value. By doing so, it is possible to suppress variations in the color image data of the standard material.

  The relationship between the enzyme used in the ELISA and the substrate is, for example, as follows.

(1) Examples of enzymes • HRP (horseradish peroxidase)
・ AP (alkaline oxidase)
(2) Examples of HRP substrates and colors-TMB: oxidized by HRP, colored blue (absorption wavelength 650 nm), and changed to yellow (absorption wavelength 450 nm) after termination reaction.

OPD: oxidized by HRP and colored yellow (absorption wavelength 492 nm).

ABTS: Oxidized by HRP and colored green (absorption wavelength 416 nm).

Luminol: chemiluminescent substrate (3) AP substrate and examples of colors pNPP: yellow color (absorption wavelength 405 nm)

BCIP-NBT: Colors blue-violet.

・ Amplex Red: Red color and fluorescence (excitation / emission maxima = 570/585 nm)
As described above, the color due to the reaction varies depending on the type of enzyme and the type of substrate. Further, the intensity of color development varies depending on the intensity of the reaction of the enzyme-labeled antigen.

(Measurement operation)
The operation of the sample measurement apparatus 1 will be described with reference to FIG.

  This embodiment is a case where an ELISA kit is used as a microplate. However, a calibration curve is generated using a standard substance in cases other than the ELISA kit, and the concentration of the measurement object, precipitation, etc. are determined based on the calibration curve. When measuring, it is possible to measure by substantially the same operation.

  First, initial setting of the sample measuring apparatus is executed (S11). For example, the default setting is to click the “Register” button displayed on the display unit 8 and, on the registration screen, the name of the examiner, the ELISA kit name (referred to as the ELISA kit name), and the standard substance corresponding to the ELISA kit name For example, a value such as density is registered. Multiple ELISA kit names and standard substance values can be registered. In addition, it is assumed that, for example, “RGB” is selected as the measurement data processing.

  FIG. 6 shows an example of a standard substance registration screen displayed on the display unit 8. As shown in FIG. 6, the concentration of, for example, an antigen of a standard substance is input as numerical data corresponding to the first and second columns of the microplate 12. The unit of numerical data is determined in advance before input, and is, for example, “percentage”.

  Next, when the reacted microplate 12 is set in the plate setting unit 2 and the plate setting unit 2 is returned into the housing 11, the imaging unit 4 performs an upper surface and entire surface of the microplate 12 on the basis of a command from the processing unit 7. Is photographed (S12). The imaging unit 4 supplies the captured color image data of the microplate 12 to the processing unit 7 as RGB data.

  The processing unit 7 supplies the color image data to the display unit 8, and the captured color image data is displayed on the first display unit 8a of the display unit 8 (S13).

  As shown in FIG. 2, in addition to the color image data, for example, the identification data of the microplate 12 read by the barcode reader 6 is displayed on the first display unit 8a.

  Thereafter, an ELISA kit name corresponding to the photographed color image data is selected from the registered ELISA kit names (S14). That is, when the “ELISA kit name” shown in FIG. 2 is clicked, a plurality of ELISA kit names are pulled down. From these ELISA kit names, an ELISA kit name corresponding to the color image data is selected.

  Further, the processing unit 7 calculates color data corresponding to each well and numerical data of one of the luminosity and luminance (hereinafter described based on the luminosity) from the photographed color image data (S15). Specifically, color image data located at the center of each well is extracted from the color image data of the entire surface of the microplate 12 that has been imaged. The extraction range is, for example, data in a circle having a predetermined diameter set in each well. A composite value of RGB contained in the extracted color image data for each well is calculated.

  Specifically, the R component, G component, and B component included in the color image data are weighted, and an average value thereof is calculated. This average value includes numerical data of color and luminous intensity of color image data.

  Further, instead of processing based on RGB, when processing based on the HSV color space is selected among the processing based on the HSV color space, HSL color space, HSB color space, and HLS color space described above, the RGB data is The data is converted into HSV data according to a known conversion formula. Among the HSV data, for example, the value of H is numerical data corresponding to the color (hue) of the sample in each well, the value of S corresponds to the saturation of the sample, and the value of V corresponds to the brightness or luminance of the sample. Numerical data to be used.

  Next, among the numerical data corresponding to the color of the sample calculated for each well and the numerical data corresponding to the luminous intensity, the numerical data of the luminous intensity corresponding to the standard substances in the first and second columns of the microplate 12 and A calibration curve is generated based on the numerical data corresponding to the concentration of the standard substance of the ELISA kit selected in S14 (S16).

  Specifically, an average value is calculated for each row with respect to numerical data of luminous intensity corresponding to the standard substances in the first and second columns. The color after the reaction is specified by the standard substance, and the color can be specified by any of RGB data and HSV data. Further, in the case of this example, since the concentration of the standard substance is changed from the A line to the H line, the color density (brightness), that is, the numerical data of the luminous intensity is changed. For this reason, a calibration curve is generated with the average value of the numerical data of luminous intensity calculated for each row as the vertical axis and the numerical data corresponding to the concentration of the standard substance of the ELISA kit as the horizontal axis.

  FIG. 7 shows an example of a calibration curve. The calibration curve is displayed on the second display unit 8b of the display unit 8.

  Next, based on the generated calibration curve, the luminosities of the substances to be measured in the third to twelfth rows are calculated as concentration numerical data (S17).

  FIG. 8 shows an example of the concentration of the sample corresponding to each well of the microplate 12 calculated based on the calibration curve shown in FIG. The numerical value data of the density is displayed on the second display unit 8b of the display unit 8.

  Thereafter, the photographed color image data is stored in the storage unit 9 (S18). Whether or not the color image data is stored in the storage unit 9 is arbitrary. The color image data is recorded in the storage unit 9 by operating the “save” button of the display unit 8 as necessary. As the format of the color image data to be recorded, for example, a bitmap, JPEG or the like can be used.

  The above operation is executed for each microplate.

(Effect of embodiment)
According to the embodiment, the entire microplate including a plurality of wells is photographed as a color image by the photographing unit 4, and the color information of the standard substance and the numerical data of the luminous intensity (brightness information) included in the color image data, A calibration curve is generated based on the numerical data of the concentration of the standard substance set in advance, and the color and concentration of the measurement target substance included in the color image data are calculated based on the calibration curve. For this reason, it is possible to measure quickly and reliably without using a filter as in the conventional absorbance measurement.

  In addition, since no filter is required, it is not necessary to replace the filter as in the conventional absorbance measurement. Therefore, the measurement work can be simplified.

  Further, in the case of conventional absorbance measurement, no information is left on the sample on the microplate used for the measurement. For this reason, in order to re-inspect, it is necessary to prepare a microplate of the same conditions again. However, in this embodiment, the color image data of the microplate imaged by the imaging unit 4 can be recorded in the storage unit 9. For this reason, it is possible to inspect again using the recorded color image at a later date.

  Further, the processing unit 7 converts RGB data included in the color image data into HSV data. HSV data can digitize hues more accurately than RGB data. For this reason, it is possible to improve measurement accuracy by using HSV data as compared with RGB data.

  In the present embodiment, the color and luminous intensity of the specimen in each well are calculated in a state where the entire microplate including a plurality of wells is photographed as a color image by the photographing unit 4. For this reason, it is not necessary to scan for each well by an optical system unlike the absorbance measurement. Therefore, it is not necessary to provide an optical system for scanning or a driving device for driving the support of the microplate, and the apparatus configuration can be simplified.

  In addition, the said embodiment demonstrated the case where this apparatus was applied to the drug sensitivity test as a measurement of an antigen antibody reaction. However, the present invention is not limited to this, and the present apparatus can be applied to, for example, an agglutination reaction measuring apparatus.

  The photographing unit 4 is not limited to a CMOS sensor, and may be any device that can perform color photographing on the entire surface of the microplate 12.

  Further, by using a high-sensitivity sensor provided with a fluorescent filter as the imaging unit 4, it is also possible to measure fluorescence from the specimen.

  By using a high-sensitivity sensor as the imaging unit 4, it is also possible to measure light emission from the specimen.

  The imaging unit 4 can also measure ultraviolet rays from the specimen by using an ultraviolet sensor, and can also measure infrared rays from the specimen by using an infrared sensor.

  In addition, the sample measuring apparatus of the present embodiment is not limited to ELISA, and by deforming the plate set unit 2 as a support, for example, measurement of reaction using a coloring reagent, measurement of turbidity, precipitation It is also possible to apply to observation and measurement of this, measurement of stained gel after electrophoresis, observation and measurement of bacteria on a petri dish, and observation and measurement of cell spheroids.

  In addition, the present invention is not limited to the above-described embodiments as they are, and can be embodied by modifying the constituent elements without departing from the scope of the invention in the implementation stage. Moreover, various inventions can be formed by appropriately combining a plurality of constituent elements disclosed in the above embodiments. For example, some components may be deleted from all the components shown in the embodiment. Furthermore, constituent elements over different embodiments may be appropriately combined.

  DESCRIPTION OF SYMBOLS 1 ... Sample measuring apparatus, 11 ... Housing | casing, 2 ... Plate set part (support body), 3a ... Keyboard (input part), 12 ... Microplate (sample container), 4 ... Imaging | photography part, 7 ... Processing part, 9 ... Memory part.

Claims (7)

A support for supporting a container having at least one first well containing a standard substance and a plurality of second wells containing a measurement object;
An imaging unit that images the entire surface of the container including the first and second wells and outputs the image as color image data;
An input unit for inputting the concentration of the standard substance in the first well as numerical data;
Numerical data corresponding to color information and brightness information included in the color image data supplied from the imaging unit, and numerical data of the concentration of the standard material input from the input unit An arithmetic processing unit including a first process for generating a calibration curve based on the calibration curve, and a second process for calculating a concentration of the measurement object included in the color image data as numerical data based on the calibration curve. A specimen measuring apparatus characterized by the above. A support that supports a container having a plurality of wells in which a specimen is stored;
An imaging unit that images the entire surface of the container including the plurality of wells and outputs the image as color image data;
A sample measuring apparatus comprising: an arithmetic processing unit that calculates numerical data corresponding to the color information and brightness information of the sample included in the color image data supplied from the imaging unit.   The specimen measurement apparatus according to claim 1, further comprising a storage unit that stores the color image data captured by the imaging unit.   The arithmetic processing unit calculates numerical data corresponding to color information and brightness information based on R (red), G (green), and B (blue) data included in the color image data. The sample measurement device according to claim 1 or 2.   The arithmetic processing unit converts R (red), G (green), and B (blue) data included in the color image data into numerical data of hue, saturation, and brightness and luminance, and the converted hue 3. The sample measuring apparatus according to claim 1, wherein numerical data of color and luminosity is calculated on the basis of data of saturation, one of brightness and brightness. The support supports a container having at least one first well in which a standard substance is accommodated and a plurality of second wells in which a measurement object is accommodated,
The imaging unit images the entire surface of the container including the first and second wells and outputs the image as color image data.
By the input unit, the concentration of the standard substance contained in the at least one first well is input as numerical data,
By the arithmetic processing unit, the color information of the standard material included in the color image data supplied from the imaging unit, the numerical data corresponding to the brightness information, and the standard material input from the input unit A specimen measurement method, comprising: generating a calibration curve based on numerical concentration data; and calculating, based on the calibration curve, the concentration of the measurement object included in the color image data as numerical data. Supporting the container having a plurality of wells in which the specimen is accommodated by the support,
Photographing the entire surface of the container including the plurality of wells by the photographing unit and outputting it as color image data,
A sample measurement method comprising: calculating numerical data corresponding to color information and brightness information of the sample included in the color image data supplied from the imaging unit by an arithmetic processing unit.

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