JP2016216090A - Constant temperature transport method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a constant temperature transport method which does not cause pressure variations due to a change of environmental temperature when packing a tissue such as a cell and installing the tissue in a sealed vessel within a constant temperature transport container.SOLUTION: A material 90 to be transported as a temperature-managing object is enclosed into a sealed bag 201 which has a volume regulating part 203 capable of changing a volume in accordance with a temperature change and is sterilized, the sealed bag is installed into a metallic closed vessel 101, the closed vessel is installed into a temperature-controlled metallic soaking container 501, pressure regulation valves 104, 404 provided on a ventilation pipe line 103 which is arranged so as to be penetrated from the closed vessel to the outside of the soaking container and a heat insulating container in such a state as to fix the soaking container into the heat insulating container 511 while arranging heat insulating material 506 around the soaking container are changed from a closed state to an open state, thereby, pressure in the closed vessel is kept constant, subsequently, temperature in the closed vessel reaches a predetermined temperature, thereafter, the pressure regulation valve is again closed and, then, the heat insulating container is transported.SELECTED DRAWING: Figure 5

Description

  The present invention relates to a constant temperature transport method for transporting a biological sample including a regenerated tissue manufactured in a cell processing facility or the like while maintaining a culture environment such as a constant temperature, humidity, and pressure.

  In regenerative medicine, a biological sample such as a regenerative tissue produced by culturing a small amount of cells is used to recover the function of a lost organ or the like. Regenerative medicine has evolved in recent years and has attracted a lot of attention, and is an epoch-making technique that is expected to have a great effect on diseases for which there has been no therapeutic method. The manufacturing process of a biological sample such as a regenerative tissue used for regenerative medicine is performed based on Good Manufacturing Practice (GMP), which is a standard for manufacturing control and quality control of pharmaceuticals and the like. Manufacture is performed in a cell processing center (CPC) and follows a standard operating procedure (SOP) that satisfies GMP. In Japan, the laws and regulations set by the Ministry of Health, Labor and Welfare have already been enforced in GMP (for example, Ministry of Health, Labor and Welfare No. 179, Yakuhatsu No. 480). Outside Japan, relevant laws and regulations are being enforced mainly by Western institutions (eg, US Food and Drug Administration, European Commission).

  In the early stage of practical application of regenerative medicine, it is assumed that the products will be manufactured at a small number of CPCs as production bases, shipped to medical institutions in various places, that is, transported, and used for treatment. In addition, the cell processing facility for manufacturing and the medical institution for performing treatment are separated from each other through a daily space that is less clean than the culture environment, even if they are in the same site. Therefore, although the distance varies, the work of transport always occurs. In the future, while the need for regenerative medicine from overseas will expand, it is expected that there will be more opportunities for transportation between overseas and Japan while maintaining quality in a safe and secure manner.

  At present, transportation of biological samples such as regenerative tissues is routinely performed in research and development. However, transportation methods based on standards (standards for proper logistics) aimed at ensuring quality in transportation and storage processes such as GDP (Good Distribution Practice), which are common in the pharmaceutical industry, have not yet been established. . Among them, there are various transport methods, and they are appropriately selected according to the cell type or the use after transport. For example, the transport temperature is maintained at the same constant temperature as the culture temperature (for example, about 37 ° C.), transported in the open air without temperature control (for example, 10 to 37 ° C.), or refrigerated. In cases where the cell metabolism is suppressed (eg, 4 ° C.) and in a frozen state (eg, −20 ° C., in liquid nitrogen), they are different from each other. Unlike the manufacturing process, the transportation process is affected by temperature, humidity, pressure, impact, vibration, and the like.

  The external environment that passes during transportation first has a different temperature compared to the culture environment. In the culture environment of the cell treatment facility, the temperature is appropriately selected according to the cell type or application. In many cases, the temperature during culture is 37 ° C. Since the thermostatic bath is used for temperature control, the temperature change width is small. On the other hand, during transportation, the temperature of the external environment varies depending on the location. Therefore, in order to eliminate the influence of the temperature of the external environment, a mechanism for keeping the temperature inside the cell transport container constant is necessary. Moreover, in order to reduce the influence of the temperature difference with the external environment, it is necessary to install a heat insulating material or the like around the cell transport container to ensure heat insulation. Furthermore, it is desirable that the time for keeping the internal temperature constant is sufficiently long. In the future, when transporting to a distant place such as overseas using an aircraft etc., unexpected troubles such as customs procedures at the airport from unloading at the airport apron side under harsh conditions, or shipping destination After arriving at a medical institution, a situation in which waiting time occurs for preparation of examination or surgery is assumed.

  Therefore, as an important requirement, temperature maintenance performance for a transported object is required for a considerably long time as a cell transport container. The reason for this is that it is assumed that it will be transported overseas in the future. In addition, it is assumed that in the medical institution after arrival, preparation for treatment is performed while the culture container is accommodated in the cell transport container. To maintain the temperature for a sufficiently long time, it is possible to increase the amount of chemical or heat storage material, but the total weight of the cell transport container also increases. As a result, the burden on transportation workers increases. In order to avoid this, it is necessary to improve heat insulation performance and heat retention efficiency.

  In Document 1, as shown in FIG. 6, the second heat insulating container 603 is disposed inside the first heat insulating container 601 with the first heat insulating container 601 and the heat transfer conductive cushioning material 602 interposed therebetween. Further, an airtight container 605 is built in the second heat insulating container 603 by a divided heat storage material box 604, and a structure in which heat insulating materials and heat conducting members are alternately stacked is formed randomly. By arranging a plurality in such a way that there is no gap in any direction, an improvement in heat insulation performance and heat retention efficiency is realized.

  On the other hand, pressure is another factor that differs between the external environment during transportation and the culture. Although the culture environment is a normal pressure of about 1 atm, the pressure in the cabin is around 0.8 atm during transportation, especially when using an aircraft. When a general culture vessel is exposed to a reduced pressure, the gas dissolved in the medium is eluted and the pH changes, which makes it unsuitable for cell growth. In addition, the culture medium may leak from the gap between the lid of the culture container and the main body. In that case, cleanliness is lost and biological contamination occurs. In order to avoid these, an airtight mechanism is necessary to eliminate the influence of pressure. Airtightness can be realized by means such as a screw or a hinge-type metal fitting. However, as the volume of the airtight object increases, the necessary parts increase and the weight of the entire cell transport container increases. As a result, the burden on transportation workers increases.

  On the other hand, in Document 1, the cell storage container is made small enough to enter the pass box, the lid is airtight, and temperature and pressure sensors are installed to ensure safety.

  However, when transporting from a cell culture facility, it is necessary to bring it out from a state at room temperature or 37 ° C. and place them in a transport container controlled to a desired temperature. Such a change in temperature is unavoidable, and the pressure in the sealed container fluctuates with the change in temperature. Similarly, when a low-temperature sealed container is opened after arriving at a transport laboratory or hospital, there is a possibility that pressure fluctuations occur due to a rapid temperature rise and cells are damaged.

  In this way, a constant temperature transportation method that copes with state changes accompanying environmental changes in operation before and after transportation is required.

Japanese Patent No. 5476556

  As described above, in the conventional constant temperature transportation method, it is necessary to eliminate the influence of pressure change when transporting by a technique for maintaining the temperature constant or by aircraft, etc., and the cells are transferred to a clean culture area in the cell treatment facility. Since it is necessary to carry a transport container, a demonstration is being carried out using a transport container that incorporates technology to ensure cleanliness.

  However, when cells are transported by air, they are stored in a sealed container in order to prevent changes in atmospheric pressure, but the operation of actually packing cells and other tissues in a laboratory or hospital and installing them in a sealed container in the transport container In the above, because the airtightness is ensured, the pressure in the cell built-in container decreases according to the temperature change when the cell tissue is taken out from the cell culture environment and brought to the low transport environment temperature. Will be damaged. Furthermore, when a low-temperature sealed container is opened in a transport laboratory or hospital, a pressure fluctuation occurs due to a rapid temperature rise and damages cells. In this way, we believe that care is necessary for pressure fluctuations in actual operation.

  However, the conventional method does not take into account pressure fluctuations accompanying rapid temperature changes when the transported object is installed in the transport container. For example, the transported object is packed in an environment of 37 ° C. When it is set in a closed container in the transport container in order to keep the temperature at 0 ° C., since it is sealed, the apparent pressure will be 897 hPa from the gas equation of state. Such a pressure drop damage needs to be cared for in the same manner as the atmospheric pressure change considered during air transportation described above.

  Accordingly, an object of the present invention is to solve the above-described problem, and when a tissue such as a cell is packed and placed in a sealed container in a constant temperature transport container, pressure fluctuation due to a change in environmental temperature does not occur. It is to provide a constant temperature transportation method.

In order to achieve the above object, a constant temperature transportation method according to one aspect of the present invention provides a temperature control object in a sterilized sealed bag having a volume adjusting unit capable of changing a volume according to a temperature change. Enclose a certain object to be transported, place the sealed bag in a metal sealed container, install the sealed container in a temperature-controlled metal soaking container, and install a heat insulating material around the soaking container. In a state where the soaking container is fixed in the heat insulating container in a state where it is disposed, the pressure regulating valve provided in the ventilation pipe arranged to penetrate from the sealed container to the outside of the soaking container and the heat insulating container is closed. Change from open to open, keep the inside of the closed container at a constant pressure,
Next, after the temperature in the sealed container reaches a predetermined temperature, the pressure regulating valve is closed again, and then the insulated container is transported.

  According to the aspect of the present invention, when the sealed container containing the object to be transported is installed in the soaking container controlled to a predetermined temperature, the pressure adjustment valve is changed from the initial closed state to the opened state. Change and keep the inside of the sealed container at a constant pressure, and after the temperature in the sealed container reaches a predetermined temperature, the pressure regulating valve is closed again and transported. The object to be transported can be transported with the temperature and pressure maintained. Moreover, when taking out the said airtight container from the said soaking | uniform-heating container, the to-be-transported object can be taken out after changing the said pressure control valve from a closed state to an open state, and maintaining the same atmospheric pressure as external air. With this configuration, there is almost no influence of pressure fluctuations during operation due to a sudden temperature change when a transported object is taken in or out of a constant temperature transport container, and transport to a processing facility such as a lab or hospital and actual It is possible to use the transported goods.

Partial sectional drawing of the structure of the airtight container in embodiment of this invention The partial cross section figure of the structure of the sealing bag containing a glass bottle in embodiment of this invention Partial sectional drawing of the structure of the sealing bag containing a petri dish in the embodiment of the present invention The partial cross section figure of the structure of the airtight container with an automatic opening / closing control function in embodiment of this invention Partial sectional drawing of the structure of the constant temperature transport container in embodiment of this invention Partial sectional view of the configuration of a conventional cell transport container

  Hereinafter, embodiments of the present invention will be described with reference to the drawings.

  FIG. 1 is a configuration diagram of an airtight container 101 used in a constant temperature transportation method according to an embodiment of the present invention.

  In FIG. 1, a transported object 90 (which is a temperature management object) that needs to be controlled in a desired temperature is placed in a metal sealed container 101 in a state of being enclosed in a sterilized sealing bag 201. . In addition, a ventilation pipe 103 extends through the lid 101a of the sealed container 101 to the outside of the container, and a pressure adjustment valve 104 is disposed at the tip. The pressure adjustment valve 104 functions as an example of an atmospheric pressure adjustment unit. After the object to be transported 90 put in the sealed bag 201 is set in the sealed container 101, when the metal lid 101a is put on the container main body 101c, it can be crimped and held via the O-ring 101b.

  According to such a configuration, the state in the sealed container 101 can absorb pressure fluctuations accompanying a temperature change after the transported object 90 is set. That is, by opening the pressure adjustment valve 104 from a state in which the pressure adjustment valve 104 is normally closed, an air flow that cancels the differential pressure from the outside air is generated, and pressure fluctuations are prevented from occurring in the sealed container 101. I can do it.

  By the way, in the conventional transportation method, there is a difference between the temperature when the transported object 90 is prepared and the set temperature when the sealed container 101 is transported. Due to this temperature difference, after the transported object 90 is set in the sealed container 101, the temperature in the sealed bag 201 changes, and the pressure in the sealed bag 201 changes accordingly. For example, when the sealed container 101 is cooled to 5 ° C. after the cell tissue that is the transported object 90 is set in the sealed bag 201 in a 37 ° C. environment, the sealed container 101 is obtained from the following gas state equation (Formula 1). The internal pressure A drops from atmospheric pressure to 897 hPa.

(273 + 37) / 1000 hPa = (273 + 5) / AhPa (formula 1)
A = 1000 hPa × 278/310
This is highly likely to cause damage to the cell, and it is necessary to eliminate such an apparent pressure change in operation.

  Therefore, the sealed bag 201 used in the constant temperature transport container 80 of the present embodiment will be described with reference to FIGS.

  First, FIG. 2 shows a case where the transported object 90 is sealed in the glass bottle 202. The glass bottle 202 functions as an example of a transported object holding unit that needs to be temperature-controlled.

  The sealed bag 201 includes a plurality of glass bottles 202 containing a transported object (which is a temperature management object) that needs to be controlled in a desired temperature, and a volume adjusting unit 203. At that time, a gap (space) filled with air of 15% or more of the total volume of the sealed bag 201 is provided between the glass bottle 202 as the volume adjusting unit 203, for example, while the sealed bag 201 is being transported, The volume of the space is changed by pressure fluctuation outside the sealing bag. A vent hole 204 is provided in the lid 202a of the glass bottle 202.

  Thereafter, when the sealed bag 201 in which the plurality of glass bottles 202 containing the transported object 90 is actually set is installed in the sealed container 101 of FIG. 1, the inside of the sealed bag 201 containing the transported object 90 is higher than room temperature. The temperature approaches the temperature of the sealed container 101 set to a low temperature. Then, after the temperature in the sealed container 101 reaches a predetermined temperature, the pressure adjustment valve 104 is closed again. Thereafter, the sealed container 101 is transported. In addition, although the pressure in the airtight container 101 falls gradually, at the same time, when the transported object 90 is installed in the constant temperature transport container 80 and when it is taken out from the constant temperature transport container 80, the pressure is in a closed state. By manually opening the adjustment valve 104, air enters the sealed container 101 from the outside air due to the differential pressure, so that the pressure in the sealed container 101 is kept constant (that is, at a constant pressure). At this time, as a reason for providing a gap filled with air of 15% or more of the total volume of the sealing bag 201 as the volume adjusting unit 203, in the future when air transported overseas, as an assumed high temperature environment, Especially in the vicinity of the Middle East, there is a possibility that the temperature exceeds 40 ° C. Therefore, the transported object 90 set at an environmental temperature near 45 ° C is then cooled and transported to a supercooled state of 0 ° C or less. Suppose.

  In this case, from the following equation of state of gas (Equation 2), the pressure drops to 85.8% with respect to the atmospheric pressure, so a fluctuation ratio of 14.2% can be considered.

(273-0) / (273 + 45) = 85.8% (Expression 2)
Therefore, when transporting while maintaining a constant temperature even in such a harsh environment, it is necessary to have a gap filled with at least 15% air as the volume adjusting unit 203. That is, for example, if the volume adjusting unit 203 has a gap (space) filled with at least 15% air during transport of the transported object 90, the volume adjusting unit 203 even if there is a pressure fluctuation of 15%. By changing the volume of the space, this can be absorbed and damage to the transported object 90, for example, cells can be prevented.

  Moreover, in the sealing part 201a (for example, the upper edge of FIG. 2) provided in the opening part of the sealing bag 201, the entry and exit of air can be blocked and the sterilized state can be maintained, thereby preventing the entry of bacteria from the outside environment. I can do it.

  Further, the case where the transported object 90 is installed in the petri dish 302 together with the culture solution as shown in FIG. 3 is considered in the same manner as in the case of the glass bottle 202 of FIG. The sealed bag 301 includes a petri dish 302 containing a transported object 90 that needs to be controlled at a desired temperature, and a volume adjusting unit 303. The petri dish 302 containing the transported object is covered with a breathable film 304. At that time, a space filled with air up to 15% of the total volume of the sealing bag 301 is provided as a volume adjusting unit 303 between the petri dish 302 and the airtight container 101 from the high temperature state. At this time, it is possible to cancel the pressure change in the sealed container 101 due to temperature fluctuation by opening the pressure adjustment valve 104.

  In this way, the pressure adjustment valve 104 is changed from the closed state to the open state, the inside of the sealed container 101 is kept at a constant pressure, and further, after the temperature in the sealed container 101 reaches a predetermined temperature, the pressure adjustment valve 104 Close again. Thereafter, the sealed container 101 is transported.

  It is also possible to automatically control the opening / closing of these pressure adjustment valves 104 instead of manually. FIG. 4 shows an automatic opening / closing control system. This automatic opening / closing control system includes an electromagnetic valve 404 instead of the pressure adjustment valve 104, and further includes a container internal pressure gauge 401, a container external pressure gauge 402, and a control unit 403. As shown in FIG. 4, the internal and external pressures of the sealed container 101 are measured by the internal pressure gauge 401 and the external pressure gauge 402, and the measurement information is transmitted to the control unit 403 in real time. At that time, the control unit 403 calculates an amount for canceling the difference between the outside air pressure and the pressure in the sealed container 101, and controls the opening degree of the electromagnetic valve 404 by the amount for canceling the calculated pressure difference, thereby closing the sealed container 101. This produces the effect of eliminating pressure fluctuations inside.

  FIG. 5 shows the structure of the constant temperature transport container 80 when the temperature of the sealed container 101 is actually transported in the present embodiment.

  The constant temperature transport container 80 includes a soaking container 501, a heat conduction block 502, a Peltier module 503, a heat sink 504, a heat radiation fan 505, a vacuum heat insulating material 506, a power supply control temperature sensor 507, and a temperature display temperature. A sensor 508, a power supply control unit 509, a battery 510, and a heat insulating container 511 are provided.

  First, the hermetic container 101 is installed in a metal soaking container 501 with good thermal conductivity. One surface of the Peltier module 503 is attached to the soaking vessel 501 through the heat conduction block 502 so as to be in contact therewith. On the opposite surface of the Peltier module 503, a heat sink 504 and a heat dissipating fan 505 are used to promote the heat entering and exiting the Peltier module 503. In addition, a vacuum heat insulating material 506 is disposed around the soaking vessel 501 without any gap to insulate it.

  With this configuration, power is supplied to the Peltier module 503 sandwiched between the heat conduction block 502 which is an example of the cooling side heat conductor and the heat sink 504 which is an example of the heat radiation side conductor, and the heat conduction block 502 is transferred from the Peltier module 503. The heat soaking vessel 501 is radiated or heated via the heat sink.

  A power supply control temperature sensor 507 is installed on the outer surface of the soaking vessel 501 and in the vicinity of the portion where the heat conduction block 502 contacts the Peltier module 503, and in the vicinity of the portion where the heat conduction block 502 contacts the Peltier module 503. Temperature is measured. In addition, a temperature display temperature sensor 508 constituted by, for example, a wireless thermometer or a thermocouple is provided at a portion showing the temperature close to the center temperature inside the soaking vessel 501 on the outer side surface of the soaking vessel 501. Is installed, and the temperature of a portion showing a temperature close to the center temperature inside the soaking vessel 501 is measured. The following temperature control is performed so that the temperature measured by the temperature display temperature sensor 508 becomes a set temperature described later. With respect to the temperature control method, a power supply control unit 509 that controls power supply to the Peltier module 503 when a certain difference occurs between the detection results of the power supply control temperature sensor 507 and the temperature display temperature sensor 508. Under the control, power of the battery 510 as an example of the power source is supplied to the Peltier module 503. Mainly, the power supply control unit 509 controls power supply to the Peltier module 503 based on the detection result of the power supply control temperature sensor 507, and corrects the power supply to the Peltier module 503 based on the detection result of the temperature display temperature sensor 508. It is configured to do. By such temperature control, the set temperature set in the power supply control unit 509 can be held during transportation.

  The soaking vessel 501, the heat conduction block 502, the vacuum heat insulating material 506, the power supply control temperature sensor 507, the temperature display temperature sensor 508, the power supply control unit 509, and the battery 510 are made of metal. It is housed and fixed in the heat insulating container 511.

  In the power supply control unit 509 of the constant temperature transport container 80, the set temperature can be set with an accuracy of 1/10 ° C. from −20.0 ° C. to 50.0 ° C. as an example. Therefore, when a certain difference occurs between the detection results of the power supply control temperature sensor 507 and the temperature display temperature sensor 508, the power supply to the Peltier module 503 is controlled under the control of the power supply control unit 509. Thus, the set temperature can be maintained during transportation regardless of fluctuations in the outside air temperature.

  Therefore, by using this constant-temperature transport container 80, the temperature and pressure of the transported object 90 installed in the sealed container 101 shown in FIG. It will be possible to transport with high accuracy and safety without any preparation.

  Therefore, when the sealed container 101 containing the object to be transported 90 is installed in the soaking container 501 controlled to a predetermined temperature, the pressure adjustment valve 104 or the electromagnetic valve 404 is changed from the closed state to the open state, and the above-described operation is performed. Since the inside of the airtight container 101 is kept at a constant pressure and the temperature inside the airtight container 101 reaches a predetermined temperature, the pressure adjusting valve 104 or the electromagnetic valve 404 is closed again for transportation. The transported object 90 can be transported in a state where a constant temperature and pressure are maintained.

  Further, when the sealed container 101 is taken out from the soaking container 501, the pressure regulating valve 104 or the electromagnetic valve 404 is changed from the closed state to the open state, and is kept at the same atmospheric pressure as the outside air. It can be taken out. If comprised in this way, there is almost no influence of the pressure fluctuation in operation accompanying the rapid temperature change at the time of taking in / out of the to-be-transported object 90 in the constant temperature transport container 80, and conveyance to processing facilities, such as a laboratory or a hospital, is possible. The actual transported object 90 can be used.

  In addition, it can be made to show the effect which each has by combining arbitrary embodiment or modification of the said various embodiment or modification suitably. In addition, combinations of the embodiments, combinations of the examples, or combinations of the embodiments and examples are possible, and combinations of features in different embodiments or examples are also possible.

  The constant temperature transport container of the present invention can be transported safely and safely with high accuracy without preparing the heat storage material or the cold insulation material while keeping the temperature and pressure of the transported object in the state before transport. It can be applied to uses such as cell transport. In the future, in order to increase opportunities for transplantation of cell sheets using Japanese regenerative medicine technology between Japan and overseas, it will be necessary to frequently transport cell tissues from donors in a constant temperature environment. For example, in the transportation to rich countries in the Middle East, it is possible to transport cells safely and easily by using the constant temperature transport container of the present invention to transport expensive transported goods. Opportunities are expected. These may lead to large businesses as a growth strategy for Japan.

80 ... constant temperature transport container 90 ... transported object 101 ... sealed container 101a ... lid 101b ... O-ring 101c ... container body 103 ... vent pipe 104 ... pressure regulating valve 201 ... Sealing bag 202 ... Glass bottle 202a ... Glass bottle lid 203 ... Volume adjustment unit 204 ... Vent 301 ... Sealing bag 302 ... Petri dish 303 ... Volume adjustment unit 304 ... Breathable film 401 ... In-container pressure gauge 402 ... Outer container pressure gauge 403 ... Control unit 404 ... Solenoid valve 501 ... Soaking container 502 ... Thermal conduction block 503 ... Peltier module 504 ... Heat sink 505 ... Heat radiation fan 506 ... Vacuum insulation 507 ... Power supply control temperature sensor 508 ... Temperature display temperature sensor 509 ... Electric control unit 510... Battery 511 .. heat insulation container 601... First heat insulation container 602 .. heat transfer conductive buffer material 603 .. second heat insulation container 604. ..Airtight containers

Claims (5)

Enclose the package to be transported, which is the object of temperature control, in a sterilized sealed bag with a volume adjustment unit that can change its volume according to temperature changes, and place the sealed bag in a metal sealed container. In the state where the airtight container is fixed in the heat insulating container in a state where the airtight container is disposed in the periphery of the heat equalizing container, the airtight container is installed in the temperature controlled metal heat equalizing container. From the closed state to the open state pressure change valve provided in the ventilation pipe arranged to penetrate to the outside of the soaking container and the heat insulating container from the closed state, keeping the inside of the sealed container at a constant pressure,
Then, after the temperature in the closed container reaches a predetermined temperature, the pressure regulating valve is closed again, and then the insulated container is transported.   2. The constant temperature transportation method according to claim 1, wherein when the sealed container is taken out from the soaking container, the pressure regulating valve is changed from a closed state to an opened state, and the inside of the sealed container is maintained at the same pressure as the outside air. .   During the transportation of the heat insulating container, the volume adjustment part of the sealed bag has a space of at least 15% or more of the total volume of the sealed bag in the state after the transported object is set. The constant temperature transportation method according to claim 1 or 2, wherein the volume of the space varies due to pressure fluctuation outside the bag. While transporting the heat insulation container, measure the temperature in the airtight container with a wireless thermometer or thermocouple installed in the airtight container,
The constant temperature transport according to any one of claims 1 to 3, wherein the temperature is controlled so that the temperature of the transported object is maintained at a temperature in a state before transport based on the measured temperature in the sealed container. Method. The soaking vessel includes a cooling side heat conductor, a heat dissipation side conductor, a Peltier module interposed between the cooling side heat conductor and the heat dissipation side conductor, a power source for supplying power to the Peltier module, and the leveling device. A temperature sensor for power supply control installed in the vicinity of the cooling side heat conductor on the outer side surface of the heat vessel or on the cooling side heat conductor, and the center temperature inside the heat equalization vessel on the outer side surface of the heat equalization vessel A temperature display temperature sensor installed at a portion showing a temperature close to the temperature, a power supply control temperature sensor, and a power supply control unit that controls power supply to the Peltier module based on a detection result of the temperature display temperature sensor so,
While transporting the insulated container, the power supply to the Peltier module is controlled based on the detection result of the power supply control temperature sensor and the detection result of the temperature display temperature sensor, and the temperature of the object to be transported is controlled before the transport. The constant temperature transport method according to any one of claims 1 to 4, wherein control is performed so as to be maintained at a temperature of the state.

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