JP2016165236A - Manufacturing method of hard cookie - Google Patents

Manufacturing method of hard cookie Download PDF

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JP2016165236A
JP2016165236A JP2015045914A JP2015045914A JP2016165236A JP 2016165236 A JP2016165236 A JP 2016165236A JP 2015045914 A JP2015045914 A JP 2015045914A JP 2015045914 A JP2015045914 A JP 2015045914A JP 2016165236 A JP2016165236 A JP 2016165236A
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mung bean
protein
hard
raw material
weight
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JP6507724B2 (en
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将宏 杉山
Masahiro Sugiyama
将宏 杉山
吉田 隆治
Takaharu Yoshida
隆治 吉田
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Fuji Oil Co Ltd
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Abstract

PROBLEM TO BE SOLVED: To provide a hard cookie less in texture deterioration by moisture absorption and having crispy texture while capable of taking beans or beans protein easily.SOLUTION: Crispy texture reduction of hard cookie by moisture absorption can be suppressed by blending a raw material derived from a mung bean dry type milled article without passing a wet type extraction process of 3 wt.% to 15 wt.% as a mung bean protein in a raw material dried article. Preferably raw material derived from the blended mung bean dry type milled article is a mung bean protein concentrate obtained by an air classification and more preferably protein content of the mung bean concentration is 35 wt.% or more.SELECTED DRAWING: None

Description

本発明は、緑豆を配合したハードクッキーに関する。   The present invention relates to a hard cookie blended with mung beans.

ハードクッキーはサクサクとした硬い食感を特長とする焼菓子である。しかし水分含量が比較的低いために吸湿しやすく、このサクサク感が失われやすいという問題を有する。
吸湿を防止するために、乾燥剤の封入や多重包装が行われているが、製造工程の煩雑化によるコストの上昇や、包材の使用による環境負荷の増加が発生する。また消費者によって一度開封されてしまうと、その後の吸湿防止が難しい。
Hard cookies are baked confectionery that features a crisp and hard texture. However, since the moisture content is relatively low, it is easy to absorb moisture, and this crispy feeling is easily lost.
In order to prevent moisture absorption, a desiccant is enclosed and multiple packaging is performed. However, an increase in cost due to complicated manufacturing processes and an increase in environmental load due to the use of packaging materials occur. Further, once opened by the consumer, it is difficult to prevent moisture absorption thereafter.

特許文献1には、結晶セルロースを添加することで、吸湿硬度低下を抑えられることが記載されている。しかし結晶セルロースは食品添加物としての表示義務があり、汎用性に欠ける。また特許文献2ではある特定の糖アルコールを添加することで、吸湿を抑制できることが記載されている。しかし特定の糖アルコールを加える必要があるため、甘味料が限定的であり、上記同様に汎用性に欠ける。   Patent Document 1 describes that a decrease in moisture absorption hardness can be suppressed by adding crystalline cellulose. However, crystalline cellulose has a duty to label as a food additive and lacks versatility. Patent Document 2 describes that moisture absorption can be suppressed by adding a specific sugar alcohol. However, since it is necessary to add a specific sugar alcohol, sweeteners are limited and lack the versatility as described above.

一方、嗜好品であるハードクッキーにおいても健康志向のニーズが存在する。中でも豆類は栄養価が高く、特に蛋白質に富んでいるため、添加素材として有望である。代表例は大豆であり、大豆蛋白質には様々な栄養生理効果が知られている。しかしこれは強い吸水性を有するため、ハードクッキーに添加すると焼成後の吸湿が促進され、特徴的なサクサクした食感の経時的な喪失が顕著となってしまう。   On the other hand, there are health-oriented needs for hard cookies, which are luxury items. Beans are particularly promising as an additive material because they have high nutritional value and are particularly rich in protein. A representative example is soybean, and various nutritional physiological effects are known for soybean protein. However, since it has a strong water absorption, when it is added to a hard cookie, moisture absorption after baking is promoted, and the characteristic crisp texture is lost over time.

大豆蛋白質の構成成分であるβ-コングリシニンは、血中中性脂肪(特許文献3)や体脂肪(特許文献4)の改善効果を有し、特定保健用食品等にも利用されている。そしてこのβ-コングリシニン様の機能は、マメ科ササゲ属の豆類である緑豆の主たる構成蛋白質にも存在することが知られている(非特許文献1)。   Β-conglycinin, which is a component of soy protein, has an effect of improving blood neutral fat (Patent Document 3) and body fat (Patent Document 4), and is also used in food for specified health use. It is known that this β-conglycinin-like function also exists in the main constituent protein of mung beans, which are legumes of the leguminous cowpea genus (Non-patent Document 1).

本出願人は先に、湿式抽出(水抽出)により得られた分離緑豆蛋白を配合することで、良好なソフトクッキーが製造可能であることを開示している(特許文献5)。しかしソフトクッキーはしっとりした食感を特徴とするものであり、ハードクッキーのサクサク食感とは異なる。   The present applicant has previously disclosed that a good soft cookie can be produced by blending separated mung bean protein obtained by wet extraction (water extraction) (Patent Document 5). However, soft cookies are characterized by a moist texture and are different from the hard texture of hard cookies.

特開平8−205778号公報JP-A-8-205778 特開2004-33109号公報JP 2004-33109 A WO2002/026243国際公開パンフレットWO2002 / 026243 International pamphlet WO2004/009107国際公開パンフレットWO2004 / 009107 International publication pamphlet WO2014/156552国際公開パンフレットWO2014 / 156552 International pamphlet

分離緑豆タンパク質の新規生理機能探索(1)―血中中性脂肪低下作用―(第68回日本栄養・食糧学会大会講演要旨集,225頁,2013年)Exploration of novel physiological functions of isolated mung bean protein (1) -blood neutral fat lowering action- (Abstracts of the 68th Annual Meeting of the Japan Society of Nutrition and Food Science, 225, 2013)

表示上の制限がある食品添加物や、風味の影響の強い食材を使うことなく、栄養成分、特に豆類蛋白質を手軽に摂取可能でありながら、吸湿による食感変化の少ないハードクッキーを提供することを課題とした。   To provide hard cookies that can easily ingest nutritional ingredients, especially legume protein, without the use of food additives that have restrictions on labeling or ingredients that have a strong influence on flavor, but that have little texture change due to moisture absorption Was an issue.

上記課題に鑑み、本発明者は鋭意研究を行った結果、豆類原料として緑豆粉をハードクッキーに配合することで高湿度下においてもサクサクとした食感が維持できることを見出した。さらに空気分級によって得られた緑豆蛋白質濃縮物を配合することで、高湿度下においてもサクサクとした食感がさらに効果的に維持され、かつ風味も良好なハードクッキーが作製できることを発見し、本発明を完成するに至った。   In view of the above problems, as a result of intensive studies, the present inventor has found that a crunchy texture can be maintained even under high humidity by blending mung bean powder into hard cookies as a raw material for beans. Furthermore, it was discovered that by adding the mung bean protein concentrate obtained by air classification, a hard cookie with a good flavor and good flavor can be produced even under high humidity. The invention has been completed.

すなわち本発明は、
(1)湿式抽出工程を経ていない緑豆乾式粉砕物由来原料を、対原料乾燥物中緑豆蛋白質として3重量%以上15重量%以下相当量配合するハードクッキーの製造方法、
(2)配合する緑豆乾式粉砕物由来原料が空気分級により得られた緑豆蛋白濃縮物である、(1)に記載のハードクッキーの製造方法。
(3)緑豆蛋白濃縮物が蛋白質含量35重量%以上であることを特徴とする(2)に記載のハードクッキーの製造方法、
(4)緑豆蛋白質濃縮物の粒径20μm以上の画分が30重量%以下であることを特徴とする、(2)ないし(3)に記載のハードクッキーの製造方法、
である。
That is, the present invention
(1) A method for producing a hard cookie, wherein a raw material derived from a dry pulverized mung bean that has not undergone a wet extraction step is blended in an amount equivalent to 3% by weight to 15% by weight as a mung bean protein in the dry material
(2) The method for producing a hard cookie according to (1), wherein the raw material derived from the mung bean dry pulverized product is a mung bean protein concentrate obtained by air classification.
(3) The method for producing a hard cookie according to (2), wherein the mung bean protein concentrate has a protein content of 35% by weight or more,
(4) The method for producing a hard cookie according to any one of (2) to (3), wherein a fraction of the particle size of 20 μm or more of the mung bean protein concentrate is 30% by weight or less,
It is.

本発明によれば、軽食や間食として手軽に蛋白質を摂取可能であり、開封後も吸湿による食感の劣化が起こりにくいハードクッキーを提供できる。   ADVANTAGE OF THE INVENTION According to this invention, the hard cookie which can take protein easily as a light meal or a snack, and does not easily deteriorate the food texture due to moisture absorption after opening can be provided.

(緑豆)
緑豆とは、マメ科ササゲ属である、ヤエナリ(八重生)(Vigna radiata)の種子である。青小豆(あおあずき)、文豆(ぶんどう)と呼称されることもある。これら緑豆について、後述する乾式粉砕を行った後に、蛋白質画分を濃縮する。蛋白質の濃縮率を高めるためには、粉砕前に、緑豆の外皮は予め除いた方が好ましい。
(Mung Bean)
Mung beans are the seeds of Vigna radiata, a leguminous cowpea genus. It is sometimes called Ao Azuki or Bun. About these mung beans, after carrying out the dry-type grinding | pulverization mentioned later, a protein fraction is concentrated. In order to increase the concentration rate of protein, it is preferable to remove mung bean hulls in advance before pulverization.

(乾式粉砕)
本発明は緑豆の乾式粉砕物を使用することを特徴とする。乾式粉砕とは、蛋白質含量が30重量%程度の緑豆、好ましくは脱皮緑豆について、例えばフレーククラッシャー,ハンマーミル,ピンミル,ブレードミル,ボールミル,スタンプミル,バンタムミル,ジェットミル,サイクロンミル,フレットミル,パンミル,エッジランナー,ローラーミル,ミックスマーラー,振動ミルなどの乾式粉砕機を用いて、緑豆を粉砕することである。そして、破砕およびその前後の工程に於いて、積極的な水の介在なく処理を行うことが特徴である。水を用いると豆細胞組織中のプロテインボディが吸水し、この後の分画作業が困難になる場合がある。好ましい乾式粉砕機として、株式会社セイシン企業のジェットミルやインペラミル、アルピネ社のピンミル等を用いることができる。
(Dry grinding)
The present invention is characterized by using a dry pulverized product of mung beans. Dry milling refers to mung beans with a protein content of about 30% by weight, preferably peeled mung beans, such as flake crusher, hammer mill, pin mill, blade mill, ball mill, stamp mill, bantam mill, jet mill, cyclone mill, fret mill, pan mill. , Pulverize mung beans using dry crushers such as edge runners, roller mills, mix mullers, and vibration mills. And in the crushing and the process before and after that, it is characterized by processing without active water intervention. When water is used, the protein body in the bean cell tissue absorbs water, and the subsequent fractionation may be difficult. As a preferred dry mill, a jet mill or impeller mill manufactured by Seishin Co., Ltd., a pin mill manufactured by Alpine Corporation or the like can be used.

緑豆乾式粉砕物は粒子径により組成が異なる特徴を持ち、粒子径の小さい画分、すなわち微粉側に蛋白質がより多く含まれるため、破砕後分級前粉砕物の粒度分布において20μm以上の画分が体積基準で好ましくは30重量%以下、より好ましくは15重量%以下となるように粉砕することで、後述する分級によって蛋白質濃度の高い緑豆蛋白濃縮物を効率的に得ることができる。なお、粒子径分布はLMS-2000e(株式会社セイシン企業)、SALD-2300(島津製作所製)などのレーザー回折式粒度分布測定装置を用いて、測定媒液を2-プロパノールで測定することができる。   The green pulverized dry pulverized product has a characteristic that the composition varies depending on the particle size, and since the fraction with a small particle size, that is, contains more protein on the fine powder side, the fraction of 20 μm or more in the particle size distribution of the pulverized product after classification after pulverization By grinding so as to be preferably 30% by weight or less, more preferably 15% by weight or less on a volume basis, a mung bean protein concentrate having a high protein concentration can be efficiently obtained by classification described later. The particle size distribution can be measured with 2-propanol using a laser diffraction particle size distribution analyzer such as LMS-2000e (Seishin Corporation), SALD-2300 (manufactured by Shimadzu Corporation). .

(分級)
緑豆乾式粉砕物は、さらに続いて微粉側の画分を分離することで、蛋白質含量の高い濃縮物を得ることができる。分離手段としてはプロテインボディからの蛋白質の溶出を避けるため、乾式分級を用いることが好ましい。乾式分級を行う好ましい装置としては、篩、振動篩、重力式分級機、サイクロンなどの遠心式分級機、ルーバー型分級機などの慣性力式分級機を使用可能であり、分級する粒度、処理速度等を考慮し、適宜選択することができる。
(Classification)
The dry pulverized product of mung beans can be further obtained by separating the fraction on the fine powder side to obtain a concentrate having a high protein content. As the separation means, dry classification is preferably used in order to avoid elution of the protein from the protein body. As a preferred apparatus for performing dry classification, an inertial force classifier such as a sieve, a vibrating sieve, a gravity classifier, a centrifugal classifier such as a cyclone, or a louver type classifier can be used. Etc. can be selected as appropriate.

(空気分級)
乾式分級は、好ましくは空気分級を行う。空気分級とは、流体分級のうち空気等の気体を流体に用いる手法であり、気流分級や風力分級とも呼ばれ、高い分画精度で大量の製品を処理できる特徴を有する。装置として、株式会社セイシン企業のクラッシールやアルピネ社のミクロプレックス等が挙げられる。
(Air classification)
In the dry classification, air classification is preferably performed. Air classification is a method of using a gas such as air as a fluid among fluid classification, and is also referred to as airflow classification or wind classification, and has a feature that a large amount of products can be processed with high fractionation accuracy. Examples of the equipment include Claseal of Seishin Co., Ltd. and Microplex of Alpine.

(緑豆蛋白濃縮物)
本発明の緑豆蛋白濃縮物は、緑豆乾式粉砕物を原料とし、湿式抽出(水抽出)することなく、前述の乾式分級、好ましくは空気分級によって特定画分を分離することで、蛋白質含量を35重量%以上に濃縮したものである。また45重量%以上が好ましく、50重量%以上が更に好ましい。蛋白質含量が高いと風味が向上する上に、生理機能の発現が期待される量を効率的に摂取し易くなり、更に本発明の特徴である、吸湿を抑制し食感を維持する効果が高い。
(Mung Bean Protein Concentrate)
The mung bean protein concentrate of the present invention uses mung bean dry pulverized material as a raw material, and does not perform wet extraction (water extraction), but separates a specific fraction by the above-mentioned dry classification, preferably air classification, to obtain a protein content of 35. Concentrated to more than wt%. Moreover, 45 weight% or more is preferable and 50 weight% or more is still more preferable. When the protein content is high, the flavor is improved and it is easy to efficiently ingest the amount expected to express physiological functions, and further, the effect of suppressing moisture absorption and maintaining the texture, which is a feature of the present invention, is high. .

また、緑豆蛋白濃縮物はその分級後の粒径が、20μm以上の画分が体積基準で30重量%以下であることが好ましく、15重量%以下であることがより好ましい。粒径が小さいと、吸湿抑制効果が高い傾向にある。   Further, the fraction of mung bean protein concentrate having a particle size of 20 μm or more is preferably 30% by weight or less, more preferably 15% by weight or less, based on volume. When the particle size is small, the moisture absorption suppressing effect tends to be high.

(緑豆蛋白質の定量方法)
緑豆蛋白質の濃度測定方法としては、ウエスタンブロッティング法を用いることができる。すなわち、摩砕したサンプルにSDS及び2-メルカプトエタノール等の還元剤を含むサンプルバッファーを加え、10分間沸騰水中で抽出する。その後、何点かの濃度に調整した緑豆蛋白質(コントロール)を用いて、サンプルと同時にSDS-PAGEを行い、セミドライ法によりPVDF(Polyvinylidene difluoride)膜に転写する。転写した膜に、一次抗体として抗緑豆蛋白質抗体を反応させ、AP (Alkaline phosphatase)又はHRP(Horse radish peroxidase)等で標識された抗体を二次抗体として一次抗体と反応させ、酵素活性による発色等により、緑豆蛋白質を定量することができる。大豆蛋白質、エンドウ蛋白質も同様の方法で定量することができる。
(Method for quantifying mung bean protein)
Western blotting can be used as a method for measuring the concentration of mung bean protein. That is, a sample buffer containing a reducing agent such as SDS and 2-mercaptoethanol is added to the ground sample and extracted in boiling water for 10 minutes. Thereafter, SDS-PAGE is performed simultaneously with the sample using mung bean protein (control) adjusted to several concentrations, and transferred to a PVDF (Polyvinylidene difluoride) membrane by a semi-dry method. The transferred membrane is reacted with an anti-mung bean protein antibody as a primary antibody, and an antibody labeled with AP (Alkaline phosphatase) or HRP (Horse radish peroxidase) or the like is reacted with the primary antibody as a secondary antibody. Thus, mung bean protein can be quantified. Soy protein and pea protein can also be quantified by the same method.

(ハードクッキー)
本発明におけるハードクッキーとは、穀粉類を主原料とし、副原料を加えて混捏した生地を成形、焼成した焼菓子のことをいう。特に、薄型に成形され、サクサクとした硬い食感がその商品価値を左右する、焼成後の水分含量が5重量%未満であるものを指す。
(Hard cookies)
The hard cookie in the present invention refers to a baked confectionery obtained by forming and baking a kneaded dough using cereal flour as a main ingredient and adding auxiliary ingredients. In particular, it refers to those that are formed into a thin shape and have a crispy, hard texture that affects their commercial value and that have a water content of less than 5% by weight after firing.

(原料穀粉)
生地の調製には、従来公知のハードクッキーに用いられる原料を特に制限されず自由に用いることができる。特に穀粉については、クッキーの製造に使用される穀粉は一般的に小麦粉を使用することが多いが、ここには大麦、ライ麦、麦芽、トウモロコシ、エンバク、米、その他豆類等の穀粉を含んでも良い。また澱粉、加工澱粉、等を含んでも良い。本発明ではこれらを原料穀粉とする。これらの原料は本発明の効果に影響を与えない範囲で使用することが出来る。
(Raw material flour)
In preparing the dough, the raw materials used in conventionally known hard cookies are not particularly limited and can be used freely. In particular, for flour, flour used in the manufacture of cookies generally uses flour, but this may include flour such as barley, rye, malt, corn, oat, rice, and other beans. . Moreover, starch, processed starch, etc. may be included. In the present invention, these are raw material flours. These raw materials can be used as long as the effects of the present invention are not affected.

(副原料)
原料穀粉以外にも、種々の副原料を加えることができる。例えば、バター、マーガリン、ショートニング等の可塑性油脂、液状油、甘味料、卵、牛乳、豆乳、チョコレート、ドライフルーツ、ナッツ、野菜、ココア、コーヒー、乳製品、各種膨張剤等の一般的な原料を使用することができる。甘味料として、上白糖、三温糖、黒砂糖、キビ砂糖、蜂蜜、メープルシロップ、アガベシロップ、糖蜜、水飴、ブドウ糖、果糖、麦芽糖、ショ糖、ブドウ糖液糖果糖、オリゴ糖、フラクトオリゴ糖、イヌリン等の糖類、アスパルテーム、アセスルファムカリウム、スクラロース、サッカリン、ネオテーム等の人工甘味料、トレハロース、甘草抽出物、ステビア抽出物、羅漢果抽出物、ソーマチン、グリセリン、クルクリン、モネリン、モナチン等を使用することができる。この他にも各種保存料等、焼き菓子で用いられる従来公知の添加物を併用してもよい。
(Sub-material)
Various auxiliary materials can be added in addition to the raw material flour. For example, general raw materials such as plastic fats and oils such as butter, margarine and shortening, liquid oil, sweetener, egg, milk, soy milk, chocolate, dried fruit, nuts, vegetables, cocoa, coffee, dairy products, various swelling agents, etc. Can be used. As sweeteners, super white sugar, tri-sugar, brown sugar, millet sugar, honey, maple syrup, agave syrup, molasses, starch syrup, glucose, fructose, maltose, sucrose, glucose liquid fructose, oligosaccharide, fructooligosaccharide, inulin Sugars such as aspartame, acesulfame potassium, sucralose, saccharin, neotame and other artificial sweeteners, trehalose, licorice extract, stevia extract, rahan fruit extract, thaumatin, glycerin, curculin, monelin, monatin, etc. can be used . In addition to these, conventionally known additives used in baked goods such as various preservatives may be used in combination.

さらに健康効果を期待し、大豆、その他の豆類、豆乳粉末、調整豆乳粉末、大豆粉、脱脂大豆粉、その他豆類の穀紛、乳、ホエー、コラーゲン等のタンパク質、タンパク質分解物又はペプチドを用いてもよく、ω-3系油脂、ビタミン、ミネラル、ポリフェノール類、アスタキサンチン、共役リノール酸、水溶性食物繊維、不溶性食物繊維、カテキン、オリーブ(葉)ポリフェノール、難消化デキストリン、シナモン、リグナン、イソフラボン、植物ステロール、アセチルシステイン、白インゲン抽出物、ファセオラミン、アラビノキシラン、フィチン酸、(ウーロン茶)ポリフェノール、エルカンプーレ、カイアポイモ、グルコマンナン、クロム、桑の葉、タマネギ、テアフラビン、ドクダミ、バナジウム、フェヌグリーク、ペクチン、ホップ、ポリデキストロース、卵黄油等の素材と組み合わせてもよい。これらは本発明の効果を阻害しない範囲で適宜配合することができる。   In addition, using soybeans, other beans, soybean milk powder, modified soybean milk powder, soybean flour, defatted soybean flour, other beans flour, milk, whey, collagen, etc. Ω-3 oils, vitamins, minerals, polyphenols, astaxanthin, conjugated linoleic acid, water-soluble dietary fiber, insoluble dietary fiber, catechin, olive (leaf) polyphenol, indigestible dextrin, cinnamon, lignan, isoflavone, plant Sterol, acetylcysteine, white kidney bean extract, phaseolamin, arabinoxylan, phytic acid, (oolong tea) polyphenol, ercampule, caiapoimo, glucomannan, chrome, mulberry leaf, onion, theaflavin, dokudami, vanadium, fenugreek, pectin, hop It may be combined with materials such as polydextrose and egg yolk oil. These can be appropriately blended within a range not impairing the effects of the present invention.

本発明の緑豆乾式粉砕物由来原料の添加量としては、ハードクッキーの原料乾燥物に対して、緑豆蛋白質として、3重量%以上15重量%以下、好ましくは7重量%以上12重量%以下である。少なすぎると経時的な食感維持の効果が出にくく、多すぎると生地としてまとまりにくくなる。なお、ここでの「原料乾燥物」とは、原料から水分量を差し引いた重量をいう。   The added amount of the raw material derived from the mung bean dry pulverized product of the present invention is 3% by weight or more and 15% by weight or less, preferably 7% by weight or more and 12% by weight or less, as the mung bean protein, with respect to the hard cookie raw material dried product. . If the amount is too small, the effect of maintaining the texture over time is difficult to obtain. The “dried material” here refers to the weight obtained by subtracting the amount of water from the raw material.

(高湿度)
本発明によれば、サクサクとした食感が維持された、豆類蛋白高配合ハードクッキーを提供できる。本効果は特に高湿度下での吸湿抑制が顕著である。高湿度下とは、例えば25℃に於ける湿度が70%以上、好ましくは80%以上の条件を指す。
(High humidity)
ADVANTAGE OF THE INVENTION According to this invention, the beans protein high mixing | blending hard cookie with which the crunchy texture was maintained can be provided. This effect is particularly remarkable in suppressing moisture absorption under high humidity. High humidity refers to, for example, a condition where the humidity at 25 ° C. is 70% or more, preferably 80% or more.

(用途)
本発明のハードクッキーは吸湿性が低いため、開封から消費まで時間がかかる用途、例えば個包装なしで大袋にて提供される商品形態等において広く応用することができる。また、栄養価に優れた緑豆蛋白質を手軽に多量に摂取することができる。さらに緑豆蛋白質は血中の中性脂肪を低減する機能を持つことも報告されているため、栄養機能的な効果も期待できる。
(Use)
Since the hard cookie of the present invention has low hygroscopicity, it can be widely applied in applications that take time from opening to consumption, such as product forms provided in large bags without individual packaging. In addition, a large amount of mung bean protein having excellent nutritional value can be easily ingested. Furthermore, since mung bean protein has been reported to have a function of reducing triglycerides in blood, a nutritional functional effect can be expected.

以下、実施例により本発明のより具体的な実施態様について説明する。   Hereinafter, more specific embodiments of the present invention will be described by way of examples.

(製造例1)緑豆乾式粉砕物、及び緑豆粉の作製
脱皮緑豆を立型グラインダ(サワーボーイNSG-15F、株式会社長沢機械製作所)で粉砕し、JIS試験篩い16メッシュを通して、20μm以上の画分が体積基準で82%の予備粉砕物を得た。これを緑豆乾式粉砕物とした。緑豆乾式粉砕物はさらにジェットミル(シングルトラック・ジェットミルFS-4、株式会社セイシン企業)で破砕し、緑豆粉とした(圧力0.5MPa)。緑豆粉の蛋白質含量は29.5重量%、20μm以上の画分が体積基準で13%であった。
(Production Example 1) Mung bean dry pulverized product and preparation of mung bean powder Dehulled mung beans were pulverized with a vertical grinder (Sourboy NSG-15F, Nagasawa Machinery Co., Ltd.), and the fraction of 20μm or more was passed through 16 mesh of JIS test sieve. However, 82% of the pre-ground product was obtained on a volume basis. This was made into a mung bean dry pulverized product. The mung bean dry pulverized product was further crushed with a jet mill (Single Track Jet Mill FS-4, Seishin Enterprise Co., Ltd.) to obtain mung bean powder (pressure 0.5 MPa). The protein content of mung bean flour was 29.5% by weight, and the fraction of 20 μm or more was 13% by volume.

(製造例2)空気分級による緑豆蛋白濃縮物(乾式)の作製
緑豆粉は気流分級機(クラッシールN-01、株式会社セイシン企業)を用いてさらに分級(空気分級)した(ローター回転数5,000rpm)。分級の結果得られた微粉側の蛋白質含量は62重量%であり、20μm以上の画分は体積基準で20重量%であった。これを緑豆蛋白濃縮物(乾式)とした。
(Production Example 2) Production of Mung Bean Protein Concentrate (Dry Type) by Air Classification Mung bean powder was further classified (air classification) using an airflow classifier (Classeal N-01, Seishin Corporation) (rotor rotation speed 5,000 rpm). The protein content on the fine powder side obtained as a result of classification was 62% by weight, and the fraction of 20 μm or more was 20% by weight on a volume basis. This was designated as mung bean protein concentrate (dry type).

(比較製造例1,2)
脱皮脱胚した大豆、またはエンドウ豆について、製造例1と同様にグラインダで粉砕し、メッシュパスした予備粉砕物を、更にジェットミルで破砕し、それぞれ大豆粉(大豆蛋白質含量40重量%),エンドウ粉(エンドウ蛋白質含量25重量%)とした。
(Comparative Production Examples 1 and 2)
The moulted and de-embedded soybean or pea was crushed with a grinder in the same manner as in Production Example 1, and the pre-pulverized product passed through the mesh was further crushed with a jet mill, soy flour (soy protein content 40% by weight), pea Flour (pea protein content 25% by weight) was used.

(比較製造例3)湿式抽出による緑豆蛋白質分離物の作製
製造例1で作製した緑豆粉に4倍量の水を加え、常温で1時間撹拌抽出し、デカンターを用いて水溶液と繊維画分を分離した。続いて、水溶液のpHを4.5とし、得られた等電点沈殿蛋白質を回収し、中和後に高温高圧加熱を行って殺菌した。更に噴霧乾燥機にて乾燥し、緑豆蛋白質分離物(湿式)を得た。この蛋白質含量は86重量%であった。
(Comparative Production Example 3) Preparation of Mung Bean Protein Isolate by Wet Extraction Add 4 times the amount of water to the mung bean powder produced in Production Example 1, stir and extract at room temperature for 1 hour, and use decanter to separate the aqueous solution and fiber fraction separated. Subsequently, the pH of the aqueous solution was set to 4.5, and the obtained isoelectric precipitation protein was recovered, and after neutralization, sterilized by high-temperature and high-pressure heating. Furthermore, it dried with the spray dryer and obtained the mung bean protein isolate (wet type). The protein content was 86% by weight.

(実施例1〜3、比較例1〜4)
緑豆粉、緑豆蛋白濃縮物(乾式)、緑豆蛋白質分離物(湿式)、あるいは大豆、エンドウの粉砕物を用いてハードクッキーを作製した。
表1の配合に従って材料を混合した後、冷蔵庫で1時間生地を寝かせた。続いてこれを成形し(厚さ5mm、直径50mm)、オーブンで170℃、20分間焼成した。
焼成後のクッキーは1時間室温で放熱し、塩化カリウム及び塩化ナトリウム(何れも、キシダ化学製特級)の飽和水溶液存在下で密閉し、25℃で静置して吸湿試験を行った。尚、25℃の密閉容器内の相対湿度は、塩化カリウムでは84.2%、塩化ナトリウムでは75.3%であった。3日後に吸湿試験を終了し、クッキーの風味及び食感の官能評価を行った。官能評価ではサクサク感及び風味について評価し、設定した基準に従って点数付けを行った。尚、豆類粉砕物を加えずに調製し、焼成後吸湿試験を行わずにそのまま密閉保存した試験区をコントロールとし、実施例および比較例と対比した。
(Examples 1-3, Comparative Examples 1-4)
Hard cookies were prepared using mung bean powder, mung bean protein concentrate (dry type), mung bean protein isolate (wet type), or pulverized soybeans and peas.
After mixing the materials according to the formulation in Table 1, the dough was laid in a refrigerator for 1 hour. Subsequently, this was molded (thickness 5 mm, diameter 50 mm) and baked in an oven at 170 ° C. for 20 minutes.
The baked cookies were radiated at room temperature for 1 hour, sealed in the presence of a saturated aqueous solution of potassium chloride and sodium chloride (both are special grades manufactured by Kishida Chemical), and left at 25 ° C. to perform a moisture absorption test. The relative humidity in the sealed container at 25 ° C. was 84.2% for potassium chloride and 75.3% for sodium chloride. After 3 days, the moisture absorption test was completed, and sensory evaluation of the flavor and texture of the cookies was performed. In sensory evaluation, the crispness and flavor were evaluated and scored according to the set criteria. A test group prepared without adding the beans pulverized product and stored in a sealed state without performing a moisture absorption test after firing was used as a control, and was compared with Examples and Comparative Examples.

(表1)(単位:g)

Figure 2016165236
(Table 1) (Unit: g)
Figure 2016165236

評価は以下の基準で行った。
○サクサク感についての評価
・4点:コントロール(無添加・密閉保存)条件と同程度のサクサク感である。
・3点:コントロール(無添加・密閉保存)条件と比較するとサクサク感はやや減少している。
・2点:コントロール(無添加・密閉保存)条件と比較するとサクサク感は減少している。
・1点:サクサク感が完全に失われている。
○味についての評価
・3点:コントロールほぼ同等であり、豆の風味は全く感じない。
・2点:豆の風味を感じるが、違和感のない程度である。
・1点:豆の風味を強く感じ、青臭い。
Evaluation was performed according to the following criteria.
○ Evaluation for crispness ・ 4 points: Crispy sensation comparable to control (no additive / sealed storage) conditions.
・ 3 points: Crispy feeling is slightly reduced compared with the control (no additive / sealed storage) conditions.
・ 2 points: Crispy feeling is reduced compared to control (no additive / sealed storage) conditions.
-1 point: The crispness is completely lost.
○ Evaluation of taste ・ 3 points: The control is almost the same, and the flavor of the beans is not felt at all.
・ 2 points: Feels the flavor of beans, but does not feel uncomfortable.
-1 point: The bean flavor is strongly felt and has a blue odor.

これら試験の結果を表1下段に示した。サクサク感及び風味評価の合計が7以上を合格とした。
緑豆粉または緑豆蛋白濃縮物(乾式)を添加することによって、他の豆類粉末に比較してサクサク感が維持できていることが示された。特に、84.2%の高湿度下では、この差が顕著になった。また、原料乾燥物に対する緑豆蛋白質量を9.8重量%から4.9重量%に低減させた場合、本効果はやや低下した。
一方、緑豆蛋白質分離物(湿式)を添加したクッキーはしっとりした食感であり、本発明の目的とするサクサク食感のハードクッキーとは異なるものであった。
The results of these tests are shown in the lower part of Table 1. The sum total of a crispy feeling and flavor evaluation set 7 or more as the pass.
It was shown that by adding mung bean powder or mung bean protein concentrate (dry type), a crisp feeling can be maintained as compared with other bean powders. In particular, this difference became significant under high humidity of 84.2%. Moreover, when the amount of mung bean protein relative to the dried raw material was reduced from 9.8 wt% to 4.9 wt%, this effect was slightly reduced.
On the other hand, the cookie to which the mung bean protein isolate (wet) was added had a moist texture and was different from the crisp texture hard cookie which is the object of the present invention.

これらの結果から緑豆粉をハードクッキーに配合することで、高湿度下においてもサクサクとした食感が維持できることが明らかとなった。さらに乾式分級により蛋白質含量を高めた緑豆蛋白濃縮物を配合することで、高湿度下においてもサクサクとした食感をより効果的に維持でき、風味も良好なハードクッキーが作製できた。   From these results, it became clear that the crunchy texture can be maintained even under high humidity by adding mung bean powder to hard cookies. Furthermore, by adding a mung bean protein concentrate with a protein content increased by dry classification, a crispy texture could be maintained more effectively even under high humidity, and a hard cookie with good flavor could be produced.

Claims (4)

湿式抽出工程を経ていない緑豆乾式粉砕物由来原料を、対原料乾燥物中、緑豆蛋白質として3重量%以上15重量%以下相当量配合する、ハードクッキーの製造方法。 A method for producing a hard cookie, comprising mixing a raw material derived from a mung bean dry pulverized product that has not undergone a wet extraction step, in an amount equivalent to 3% by weight or more and 15% by weight or less as a mung bean protein. 配合する緑豆乾式粉砕物由来原料が、空気分級により得られた緑豆蛋白濃縮物である、請求項1に記載のハードクッキーの製造方法。 The manufacturing method of the hard cookie of Claim 1 whose mung bean dry-type ground material origin raw material to mix | blend is the mung bean protein concentrate obtained by the air classification. 緑豆蛋白濃縮物が、蛋白質含量35重量%以上であることを特徴とする、請求項2に記載のハードクッキーの製造方法。 The method for producing a hard cookie according to claim 2, wherein the mung bean protein concentrate has a protein content of 35% by weight or more. 緑豆蛋白質濃縮物の粒径20μm以上の画分が30重量%以下であることを特徴とする、請求項2ないし3に記載のハードクッキーの製造方法。 The method for producing a hard cookie according to any one of claims 2 to 3, wherein a fraction having a particle size of 20 µm or more of the mung bean protein concentrate is 30 wt% or less.
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CN112293454A (en) * 2020-11-03 2021-02-02 四川农业大学 Mulberry leaf biscuit and preparation method thereof

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CN107593842A (en) * 2017-10-26 2018-01-19 广东琪昌食品有限公司 A kind of high accounting mung bean soda cracker and its processing method
CN112293454A (en) * 2020-11-03 2021-02-02 四川农业大学 Mulberry leaf biscuit and preparation method thereof

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