JP2016158578A5 - - Google Patents

Claims (8)

A flavin-binding glucose dehydrogenase comprising a protein having the following amino acid sequence (a), (b) or (c) and having glucose dehydrogenase activity:
(A) the amino acid sequence shown in SEQ ID NO: 2 or 3;
(B) an amino acid sequence in which 1 or at most 5 amino acids are deleted, substituted or added in the amino acid sequence shown in SEQ ID NO: 2 or 3;
(C) an amino acid sequence having at least 90% identity with the amino acid sequence shown in SEQ ID NO: 2 or 3. A polynucleotide comprising the following (i), (ii), (iii), (iv) or (v):
(I) a polynucleotide encoding the protein of claim 1;
(Ii) a polynucleotide having the base sequence represented by SEQ ID NO: 1;
(Iii) a polynucleotide encoding a protein having a base sequence in which one or several bases are deleted, substituted or added in the base sequence shown in SEQ ID NO: 1 and having glucose dehydrogenase activity;
(Iv) a polynucleotide that hybridizes with a polynucleotide having the base sequence shown in SEQ ID NO: 1 under a stringent condition and encodes a protein having glucose dehydrogenase activity;
(V) a polynucleotide encoding a protein having a nucleotide sequence having at least 90 % identity with the nucleotide sequence shown in SEQ ID NO: 1 and having glucose dehydrogenase activity.   A recombinant vector comprising the polynucleotide according to claim 2.   A transformed cell transformed with the vector according to claim 3.   A method for producing a flavin-binding glucose dehydrogenase, comprising culturing the cell according to claim 4 and collecting flavin-binding glucose dehydrogenase from the culture.   A method for measuring glucose using the flavin-binding glucose dehydrogenase according to claim 1.   A glucose measurement reagent composition comprising the flavin-binding glucose dehydrogenase according to claim 1.   A biosensor for glucose measurement comprising the flavin-binding glucose dehydrogenase according to claim 1.