JP2016145867A - Pathological specimen type optical device and microscope - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a pathological specimen type optical device incorporating an LED used for the calibration of the color balance of the image of a pathological specimen displayed on the basis of an imaging signal outputted from the imaging element of a digital microscope device or a whole slide image creation device and the color balance of illumination light with which the pathological specimen is illuminated.SOLUTION: The pathological specimen type optical device includes a rectangular flat plate base material 4, an LED 6 which is arranged in one surface of the flat plate base material 4, an adjustment part 8 which adjusts the light quantity of the LED 6 arranged in the flat plate base material 4, and a power supply 12 which supplies electric power to the LED 6.SELECTED DRAWING: Figure 1

Description

  The present invention relates to calibration of the color balance of a pathological specimen image displayed based on an imaging signal output from an imaging device and the color balance of illumination light that illuminates the pathological specimen in a digital microscope apparatus or a hall slide image creation apparatus. The present invention relates to a pathological specimen optical device used and a microscope for magnifying and observing a pathological specimen.

  In recent years, in the field of pathology and the like, an apparatus for converting an image observed with an optical microscope into digital data and observing it on a display is used. Currently, there are digital microscope devices, hole slide image creation devices, and the like as such devices, and they are beginning to attract attention as advanced technologies in cancer countermeasures.

  Here, the digital microscope apparatus is an optical microscope that allows a pathological specimen to be observed on a display via a digital camera. The hole slide image creation device is a device that captures the entire pathological specimen on a slide glass (preparation) in which the pathological specimen is enclosed, and generates digital image data. The entire image is digitized and stored on a hard disk, DVD, or the like. Accumulated. As a specific example of the hole slide image creation device, for example, a slide image data creation device described in Patent Document 1 is known.

JP 2010-20329 A

  By the way, in the digital microscope apparatus and the hall slide image creation apparatus, the difference in color reproducibility between the imaging apparatus that captures the pathological specimen, the display apparatus that displays the imaging result, the type of the illumination apparatus that illuminates the pathological specimen, and the illumination apparatus. Since the pathological specimen is observed in different colors due to the influence of the filter arranged in the optical path between the pathological specimen and the pathological specimen, it may be difficult to make an accurate pathological diagnosis.

  An object of the present invention is to calibrate the color balance of an image of a pathological specimen displayed based on an imaging signal output from an imaging element of a digital microscope apparatus or a hall slide image creation apparatus, and the color balance of illumination light that illuminates the pathological specimen. It is providing the pathological specimen type optical device which incorporated LED used for, and a microscope.

  The pathological specimen optical device of the present invention includes a rectangular flat plate base material, LEDs arranged on one surface of the flat plate base material, and an adjustment unit that adjusts the light quantity of the LEDs arranged on the flat plate base material. And a power supply for supplying power to the LED.

  The microscope of the present invention is a microscope that illuminates a pathological specimen with illumination light emitted from the full-color LED and magnifies it, and is a red that constitutes the full-color LED based on the color of a staining reagent that stains the pathological specimen. A light amount adjusting unit that adjusts the light amount of each of the LED, the green LED, and the blue LED is provided.

  ADVANTAGE OF THE INVENTION According to this invention, the color balance of the image of the pathological specimen displayed based on the imaging signal output from the imaging device of a digital microscope apparatus or a hall slide image creation apparatus, and the calibration of the color balance of the illumination light which illuminates a pathological specimen It is possible to provide a pathological specimen type optical device incorporating an LED used for the above-mentioned and a microscope.

It is the figure which looked at the pathological specimen type optical device concerning a 1st embodiment from the upper part. It is a figure which shows the cross section of the pathological specimen type | mold optical device which concerns on 1st Embodiment. It is a block diagram which shows the structure of the digital microscope apparatus which concerns on 1st Embodiment. It is a flowchart for demonstrating the color balance calibration process which calibrates the color balance of the image of the pathological sample displayed on a display part using the pathological sample type optical device which concerns on 1st Embodiment. It is a flowchart for demonstrating the color balance calibration process which calibrates the color balance of the illumination light which illuminates the pathological specimen which concerns on 1st Embodiment. It is the figure which looked at the pathological specimen type optical device concerning a 2nd embodiment from the upper part. It is a figure which shows the structure of the other pathological specimen type | mold optical device which concerns on embodiment. It is a block diagram which shows the structure of the digital microscope apparatus which concerns on 3rd Embodiment.

  The pathological specimen optical device according to the first embodiment will be described below with reference to the drawings. FIG. 1 is a view of a pathological specimen optical device according to the first embodiment viewed from above, and FIG. 2 is a cross-sectional view thereof. As shown in FIGS. 1 and 2, the pathological specimen optical device 2 has an appearance substantially the same size as the preparation. That is, the pathological specimen optical device 2 includes a flat base material 4 having a rectangular shape made of a metal such as an aluminum material, and an LED 6 and a switch 8 are disposed on one surface of the base material 4. .

  The pathological specimen optical device 2 includes a white LED 6w, a blue LED 6b, a green LED 6g, and a red LED 6r as the LEDs 6. Here, the LED 6 is disposed in a concave portion 4a formed in a substantially central portion of one surface of the base material 4, and heat conduction grease or the like is placed in the concave portion 4a so that heat at the time of light emission is transmitted to the base material 4. It is in close contact.

  Further, as the switch 8, a switch 8w for adjusting the amount of white LED light emitted from the white LED 6w, a switch 8b for adjusting the amount of blue LED light emitted from the blue LED 6b, and the amount of green LED light emitted from the green LED 6g. A switch 8g for adjusting the amount of red LED light emitted from the red LED 6r. Here, the switch 8w, the switch 8b, the switch 8g, and the switch 8r are respectively an operation unit 8x that changes the light amount level of the LED light to the “+” side or the “−” side, and the light amount level of the LED light as a numerical value. A display unit 8y for displaying is provided. Further, the switch 8w, the switch 8b, the switch 8g, and the switch 8r are connected to a power control unit (not shown) that controls lighting, extinction, and light amount of the LED 6.

  Further, a battery 12 is disposed at a predetermined position of the base material 4 and supplies power to the LED 6 via the switch 8.

  Note that a heat sink groove 4b is formed at the peripheral edge of the back surface of the base material 4 by cutting the back surface of the base material 4 into a rectangular cross section. The cross-sectional shape of the heat sink groove 4b may be a wave shape instead of a rectangular shape. Thereby, the heat radiation area of the base material 4 is expanded, and the heat generated when the LED 6 emits light and transmitted to the base material 4 can be effectively radiated. Further, by providing the heat sink groove 4 b on the back surface of the base material 4, the cover glass 14 can be attached to the surface of the base material 4.

  FIG. 3 is a block diagram illustrating a configuration of the digital microscope apparatus according to the first embodiment. As shown in FIG. 3, the digital microscope apparatus 22 is placed on the stage 26 on which the preparation or the pathological specimen optical device 2 is placed, the microscope unit 28 for observing the preparation placed on the stage 26, and the stage 26. An illumination unit 30 including an illumination light source 30a for illuminating the prepared slide from below is provided.

  Here, the microscope unit 28 is an objective lens 28a for observing the preparation at a predetermined magnification, a lens barrel unit 28b, and an image of a pathological specimen on the preparation imaged by the objective lens 28a attached to the upper part of the lens barrel unit 28b. Is provided with a camera 28c having an image sensor 28d constituted by a CCD or the like.

  The illumination unit 30 also includes a full-color LED 30a, a lens 30b that collects illumination light emitted from the full-color LED 30a, a filter 30c that transmits illumination light collected by the lens 30b, and illumination light that has passed through the filter 30c. The mirror 30d is provided to reflect in the direction. The full color LED 30a includes a red LED, a green LED, and a blue LED.

  In addition, the digital microscope apparatus 22 includes a control unit 34 that comprehensively controls each unit of the digital microscope apparatus 22. The control unit 34 includes an imaging device 28d in the camera 28c, a light amount control unit 32, an operation unit 50 including a power switch, a display unit 40 that displays an image based on image data captured by the imaging device 28d, and a display unit. A display control unit 42 that controls display of 40 and an image storage unit 48 that stores image data captured by the image sensor 28d are connected. The image sensor 28d images a pathological specimen on the slide and outputs an imaging signal to the control unit 34. The light quantity control unit 32 controls lighting, extinction, and light quantity of each of the red LED, the green LED, and the blue LED included in the full color LED 30a.

  As shown in FIG. 3, a light receiving unit 38 a of an optical fiber 38 is disposed near the upper portion of the pathological specimen optical device 2, and light emitted from the LED 6 of the pathological specimen optical device 2 through the optical fiber 38 is received. It is transmitted to the optical spectrum analyzer 36. The optical spectrum analyzer 36 analyzes and outputs the wavelength characteristic of the LED light emitted from the LED 6 of the pathological specimen type optical device 2 acquired via the optical fiber 38. Similarly, one end of the optical fiber 39 is arranged near the upper portion of the display unit 40, and light emitted from the screen of the display unit 40 is transmitted to the optical spectrum analyzer 36 via the optical fiber 39. The optical spectrum analyzer 36 analyzes and outputs the wavelength characteristic of the image displayed on the display unit 40 acquired via the optical fiber 39.

  Next, a color balance calibration process for calibrating the color balance of the image of the pathological specimen displayed on the display unit 40 using the pathological specimen optical device 2 according to the first embodiment with reference to the flowchart shown in FIG. Will be described.

  First, when the operator turns off the power of the full color LED 30a via the operation unit 50 (step S10), the control unit 34 controls the light amount control unit 32 to turn off the full color LED 30a (step S20).

  Next, when the operator places the pathological specimen optical device 2 to which the cover glass 14 is attached on the stage 26 (step S11), the imaging device 28d images the pathological specimen optical device 2 and controls the imaging signal. To the unit 34. The control unit 34 displays the image of the pathological specimen type optical device 2 imaged by the imaging element 28d on the display unit 40 via the display control unit 42 (step S21).

  Next, the operator turns on the optical spectrum analyzer 36 (step S12) and operates the switch 8 of the pathological specimen optical device 2 (step S13). Specifically, by operating each of the switch 8w, the switch 8b, the switch 8g, and the switch 8r, the amount of white LED light emitted from the white LED 6w, the amount of blue LED light emitted from the blue LED 6b, and the green LED 6g. The amount of green LED light emitted from the red LED 6 and the amount of red LED light emitted from the red LED 6r are adjusted. In this embodiment, color balance calibration of the display unit 40 is performed using blue LED light. Accordingly, the operator operates the switches 8w, 8g, and 8r to turn off the power of the white LED 6w, the green LED 6g, and the red LED 6r, and operates the switch 8b to turn on the power of the blue LED 6b, and turns on the blue LED 6b. Further, the operator increases the amount of blue LED light by operating “+” of the switch 8b. Further, the amount of blue LED light is decreased by operating “−” of the switch 8b. Thereby, the light quantity of blue LED light can be made into a desired light quantity, and the numerical value corresponding to the light quantity is displayed on the display part 8y.

  Next, the optical spectrum analyzer 36 analyzes the wavelength characteristic of the blue LED light emitted from the blue LED 6b of the pathological specimen optical device 2 (hereinafter referred to as the wavelength characteristic of the pathological specimen optical device 2), and the analysis result is analyzed. Output. The operator acquires the analysis result of the wavelength characteristic of the pathological specimen optical device 2 output from the optical spectrum analyzer 36 (step S14).

  Similarly, the optical spectrum analyzer 36 analyzes the wavelength characteristic of the image displayed on the display unit 40 (referred to as the wavelength characteristic of the display unit 40), and outputs the analysis result. That is, the blue LED light emitted from the blue LED 6b is captured by the image sensor 28d, converted into image data by the control unit 34, and displayed on the display unit 40 as an image based on the image data. The optical spectrum analyzer 36 analyzes the wavelength characteristic of the image of the blue LED light displayed on the display unit 40 and outputs the analysis result. The operator acquires the analysis result of the wavelength characteristic of the display unit 40 output from the optical spectrum analyzer 36 (step S15).

  Next, the operator compares the wavelength characteristic of the pathological specimen optical device 2 with the wavelength characteristic of the display unit 40 (step S16). As a result of comparison, if the wavelength characteristics of the pathological specimen optical device 2 and the wavelength characteristics of the display section 40 are different (Yes in step S17), the wavelength characteristics of the display section 40 are the wavelengths of the pathological specimen optical device 2. The color balance constituted by the luminance and chromaticity of the image displayed on the display unit 40 is adjusted so as to obtain the characteristics. Specifically, in order to adjust the color balance of the image displayed on the display unit 40, the operator operates the operation unit 50 to instruct color balance adjustment (step S18). Based on an adjustment instruction from the operator via the operation unit 50, the control unit 34 controls the display control unit 42 to change the color balance of the image displayed on the display unit 40 (step S22). Thereby, the wavelength characteristic of the image of the blue LED light displayed on the display unit 40 is matched with the wavelength characteristic of the blue LED light emitted from the blue LED 6b of the pathological specimen type optical device 2.

  The light emitted from the pathological specimen type optical device 2 is affected by the colors of optical members such as the cover glass 14 and the objective lens 28a by passing through the cover glass 14, the objective lens 28a, and the lens barrel portion 28b. Further, the color of the light emitted from the pathological specimen optical device 2 and the color of the image displayed on the display unit 40 are often different due to the difference in color reproducibility between the imaging element 28d and the display unit 40. However, by using the pathological specimen optical device 2 according to the present embodiment, the color balance of the image displayed on the display unit 40, that is, the color balance of the image displayed based on the imaging signal output from the imaging element 28d is adjusted. Can be calibrated. Therefore, even when the optical member, the image sensor 28d, and the display unit 40 are affected as described above, the color of the light emitted from the pathological specimen optical device 2 and the image displayed on the display unit 40 The color can be the same.

  In the above-described color balance calibration process of the display unit 40, the color balance of the display unit 40 is calibrated using the pathological specimen type optical device 2 to which the cover glass 14 is attached, but observation is performed using the digital microscope apparatus 22. When the cover glass 14 is not included in the preparation, the color balance of the display unit 40 may be calibrated using the pathological specimen type optical device 2 with the cover glass 14 removed.

  Next, a color balance calibration process for calibrating the color balance of the illumination light that illuminates the pathological specimen according to the first embodiment will be described with reference to the flowchart shown in FIG. The illumination light color balance calibration process is performed after the color balance calibration process of the display unit 40 described above.

  After performing the color balance calibration process of the display unit 40, the operator removes the pathological specimen optical device 2 from the stage 26 (step S30). Then, the operator operates the operation unit 50 to turn on the full color LED 30a (step S31). Thereby, the control part 34 controls the light quantity control part 32, and turns on full color LED30a (step S40). In this embodiment, it is assumed that color balance calibration of illumination light is performed using blue LED light. Therefore, the operator operates the operation unit 50 so that the full color LED 30a emits blue LED light. Based on an adjustment instruction from the operator via the operation unit 50, the control unit 34 controls the light amount control unit 32 to reduce the light amounts of the red LED and the green LED constituting the full color LED 30a to 0, and to reduce the light amount of the blue LED. adjust.

  Light whose light amount is adjusted by the light amount control unit 32 and emitted from the full-color LED 30a is captured by the imaging element 28d, converted into image data by the control unit 34, and displayed on the display unit 40 as an image based on the image data. The optical spectrum analyzer 36 analyzes the wavelength characteristics of the image displayed on the display unit 40 (hereinafter referred to as wavelength characteristics before adjustment) and outputs the analysis result. The operator acquires the analysis result of the wavelength characteristic before adjustment output from the optical spectrum analyzer 36 (step S32).

  Next, the operator compares the wavelength characteristic before adjustment with the wavelength characteristic of the display unit 40 after color balance adjustment in step S18 of FIG. 4 (hereinafter referred to as target wavelength characteristic) (step S33). ). As a result of the comparison, if the wavelength characteristic before the adjustment and the target wavelength characteristic are different (Yes in step S34), the operator selects the full-color LED 30a so that the wavelength characteristic before the adjustment becomes the target wavelength characteristic. The amount of light of each of the red LED, the green LED, and the blue LED that constitute the LED is adjusted. Specifically, in order to adjust the light amounts of the red LED, the green LED, and the blue LED constituting the full color LED 30a, the operator operates the operation unit 50 to give an instruction for adjusting the light amount (step S35). Based on the adjustment instruction from the operator via the operation unit 50, the control unit 34 controls the light amount control unit 32 to change the respective light amounts of the red LED, the green LED, and the blue LED that constitute the full color LED 30a (step). S41). Thereby, the wavelength characteristic before adjustment is matched with the target wavelength characteristic.

  By matching the wavelength characteristics before adjustment with the target wavelength characteristics, the color of the light emitted from the full-color LED 30a and the color of the image displayed on the display unit 40 become the same. That is, the color of the illumination light emitted from the full-color LED 30a and illuminating the stage 26 is the same as the color of the image displayed on the display unit 40.

  The light emitted from the full-color LED 30a is affected by the colors of these optical members by passing through the lens 30b, the filter 30c, and the mirror 30d. However, by calibrating the color balance of the illumination light emitted from the full-color LED 30a as described above, the illumination light emitted from the full-color LED 30a and illuminates the stage 26 even if there is an influence of the optical member as described above. And the color of the image displayed on the display unit 40 can be made the same.

  According to the pathological specimen optical device 2 according to the first embodiment, the color balance of the image displayed on the display unit 40 can be easily calibrated. That is, in order to match the color of the pathological specimen with the color of the image displayed on the display section 40, the color balance calibration processing of the image displayed on the display section 40 using the pathological specimen type optical device 2 can be performed accurately and easily. It can be carried out.

  Next, a pathological specimen optical device according to a second embodiment will be described with reference to the drawings. In the pathological specimen optical device according to the second embodiment, a communication unit capable of communicating with an external controller or the like that remotely operates the switch 8 is added to the pathological specimen optical device 2 according to the first embodiment. Is. Therefore, in the second embodiment, portions different from the first embodiment will be described in detail, and descriptions of overlapping portions will be omitted as appropriate.

  FIG. 6 is a view of the pathological specimen optical device 102 as viewed from above. As shown in FIG. 6, the pathological specimen optical device 102 includes a communication unit 10 that is electrically connected to the power supply controller of the pathological specimen optical device 102. Here, the communication unit 10 can communicate with an external controller (not shown) by wireless communication such as WiFi (registered trademark) or Bluetooth (registered trademark). The communication unit 10 transmits the operation signal received from the controller to the power supply control unit, and the power supply control unit controls lighting, extinction, and light amount of the LED 6 based on the received operation signal. That is, the operator can remotely operate the switch 8 of the pathological specimen optical device 102 using the controller. The display unit 8y displays a numerical value corresponding to the amount of light only when the switch 8 is directly operated. That is, when the switch 8 is remotely operated using an external controller, the display function on the display unit 8y can be stopped because the operator does not visually recognize the display on the display unit 8y.

  The pathological specimen optical device 102 is a display unit of a microscope apparatus (hole slide image creation apparatus or the like) having a configuration in which the operator cannot operate the switch 8 after the pathological specimen optical device 102 is placed on the stage. It is used when calibrating the color balance of the pathological specimen image displayed on the screen. Hereinafter, a case where the color balance of an image of a pathological specimen displayed on the display unit of the hall slide image creation apparatus is calibrated using the pathological specimen optical device 102 will be described as an example.

  First, the full color LED for pathological specimen illumination provided in the hall slide image creation device is turned off. Next, after the operator places the pathological specimen optical device 102 on the stage that is retracted from the optical path, the stage is moved and placed on the optical path. When the stage is placed on the optical path, an image of the pathological specimen optical device 102 captured by the imaging device included in the hall slide image creation device is displayed on the display unit. In the state where the stage is disposed on the optical path, the operator cannot directly operate the switch 8 of the pathological specimen type optical device 102.

  Next, the operator turns on the optical spectrum analyzer and remotely operates the switch 8 using an external controller. Specifically, the communication unit 10 receives the operation signal transmitted from the controller, outputs a control signal to the power source control unit of the pathological specimen optical device 102, and the power control unit of the pathological specimen optical device 102 Based on the control signal, the LED 6 is turned on / off and the amount of light is controlled. In this embodiment, color balance calibration of the display unit 40 is performed using blue LED light. Therefore, the operator remotely operates the switches 8w, 8g, and 8r using an external controller to turn off the white LED 6w, the green LED 6g, and the red LED 6r, and remotely operates the switch 8g to turn on the blue LED 6b. Then, the blue LED 6b is turned on. Further, the operator increases the amount of blue LED light by remotely operating the “+” of the switch 8b using an external controller. Further, the amount of blue LED light is reduced by remotely operating the “−” of the switch 8b using an external controller. Thereby, the light quantity of blue LED light can be made into a desired light quantity. The subsequent processing is the same as steps S14 to S18 and S22 shown in FIG.

  When calibrating the color balance of the illumination light that illuminates the pathological specimen, the stage arranged on the optical path is moved, and the pathological specimen optical device 102 arranged on the stage is removed. The subsequent processing is the same as steps S31 to S35 and S40 to S41 shown in FIG.

  According to the pathological specimen optical device 102 according to the second embodiment, since remote control using a controller is possible, even if the switch 8 cannot be directly operated, the LED 6 is turned on and off, and the LED 6 It is possible to change the level of the amount of LED light emitted from the projector. In addition, the color balance of the image displayed on the display unit can be easily calibrated. In other words, in order to match the color of the pathological specimen with the color of the image displayed on the display section, the color balance calibration processing of the image displayed on the display section is accurately and easily performed using the pathological specimen type optical device 102. Can do.

  In each of the above-described embodiments, when the color balance of the illumination light is calibrated, the pathological specimen optical device 104 is removed from the stage. For example, a pathological specimen optical device 104 as shown in FIG. It is also possible to calibrate the color balance of the illumination light without removing the pathological specimen type optical device from the stage. A window 105 is provided on the pathological specimen optical device 104 as shown in FIG. In this case, after calibrating the color balance of the image displayed on the display unit, the stage on which the pathological specimen type device 104 is placed is moved, and the window 105 is placed on the optical path. And the wavelength characteristic of the illumination light which passed the window 105 is acquired, and the color balance of illumination light is calibrated.

  Further, in each of the above-described embodiments, the case where four types of LEDs 6 are arranged on the surface of the base material 4 is described as an example. LEDs may be arranged. That is, one type of LED (for example, only white LED 6w), two types of LEDs (for example, only red LED 6r and green LED 6g) or three types of LEDs (for example, red LED 6r, green LED 6g, and blue LED 6b) are arranged on the surface of base material 4. The color balance of the display unit 40 may be calibrated using the pathological specimen type optical device. In this case, the manufacturing cost can be reduced, and an inexpensive pathological specimen type optical device can be provided.

  In each of the above-described embodiments, a plurality of through holes may be formed in the base material 4 so that the heat transmitted to the base material 4 can be radiated more effectively.

  Further, in each of the above-described embodiments, a plurality of recessed portions whose cross section is processed into a concave shape may be formed on the back surface of the base material 4. Thereby, the heat dissipation efficiency of the base material 4 and the strength of the base material 4 can be increased.

  Further, the pathological specimen optical device according to each of the above-described embodiments includes a base material formed of a metal such as an aluminum material, but a base material formed of a material other than metal may be used. .

  Next, a digital microscope apparatus according to a third embodiment will be described with reference to the drawings. FIG. 8 is a block diagram showing a configuration of a digital microscope apparatus according to the third embodiment. Note that in the digital microscope apparatus according to the third embodiment, the same components as those of the digital microscope apparatus 22 shown in FIG.

  As shown in FIG. 8, the digital microscope apparatus 52 includes a storage unit 54 that stores information on the type of staining reagent for staining a pathological specimen to be pathological specimen, the type of pathological specimen, and the color of the pathological specimen. The storage unit 54 is connected to the control unit 34. The color of the pathological specimen, specifically the color of the stained part of the pathological specimen, is determined by the type of staining reagent and the type of pathological specimen, and the pathological specimen differs even when stained with the same staining reagent. The color of the stained part of the pathological specimen may be different. Further, even in the same pathological specimen, if the staining reagent is different, the color of the stained portion of the pathological specimen may be different. The storage unit 54 stores a table that is a list of colors of the stained portion of the pathological specimen corresponding to the type of staining reagent and the type of pathological specimen.

  When the type or the like of the staining reagent is designated by the operation unit 50, the control unit 34 determines the color of the illumination light emitted from the full color LED 30a based on the information stored in the storage unit 54, and the light amount control unit 32. To adjust the color of the illumination light emitted from the full color LED 30a.

  Hereinafter, the process of adjusting the color of the illumination light emitted from the full-color LED 30a based on the color of the staining reagent using the digital microscope apparatus 52 according to the third embodiment will be described.

  First, the operator operates the operation unit 50 to input the type of staining reagent and the type of pathological specimen used for the pathological specimen of the preparation 24. When the type of the staining reagent and the type of the pathological specimen are specified by the operation unit 50, the control unit 34 indicates the pathology corresponding to the type of the staining reagent and the type of the pathological specimen specified from the table stored in the storage unit 54. Identify the color of the stained part of the specimen. Then, the light quantity control unit 32 is controlled so that the color of the illumination light emitted from the full-color LED 30a is the color of the stained part of the pathological specimen or the color of the stained part of the pathological specimen, so that the full color Each light quantity of red LED, green LED, and blue LED which constitutes LED30a is adjusted.

  Whether the operator operates the operation unit 50 to change the color of the illumination light emitted from the full-color LED 30a to the color of the stained part of the pathological specimen or the color complementary to the color of the stained part of the pathological specimen. Can be entered. Based on the input signal from the operation unit 50, the control unit 34 specifies and adjusts the color of the illumination light emitted from the full-color LED 30a. If the pathological specimen is illuminated with illumination light having the same color as the color of the stained portion, the stained portion cannot be viewed on the display unit 40. On the other hand, when the pathological specimen is illuminated with illumination light having the same color as the complementary color of the stained portion, the stained portion can be clearly viewed on the display unit 40.

  According to the digital microscope apparatus 52 according to the third embodiment, since the color of the illumination light emitted from the full-color LED 30a can be adjusted to the color of the stained part of the pathological specimen, the color of the illumination light is stained. It is possible to prevent the displayed portion from being displayed on the display unit 40. Further, since the color of the illumination light emitted from the full color LED 30a can be adjusted to the complementary color of the color of the stained part of the pathological specimen, the part stained with the complementary color of the color of the illumination light is clearly displayed on the display unit 40. Can be displayed.

  In the above-described embodiment, the operator inputs the type of staining reagent and the type of pathological specimen, and the control unit 34 specifies the color of the stained part of the pathological specimen. It is also possible to directly input the color of the stained portion of the pathological specimen by operating the unit 50. In this case, when the control unit 34 obtains the color of the stained portion of the pathological specimen from the operation unit 50, the color of the portion of the illumination light emitted from the full-color LED 30a or the stained portion. The light quantity control unit 32 is controlled to adjust the color of the illumination light emitted from the full color LED 30a so as to be a complementary color.

  In the above-described embodiment, the digital microscope apparatus 52 has been described as an example. However, a microscope (hole slide image creation apparatus or the like) other than a digital microscope apparatus provided with a full-color LED as an illuminating apparatus that illuminates a pathological specimen is described. The present invention can also be applied to (including).

  2,102 ... Pathologic specimen type optical device, 4 ... base material, 4a ... concave, 4b ... heat sink groove, 6 ... LED, 6w ... white LED, 6b ... blue LED, 6g ... green LED, 6r ... red LED, 8 ... Switch, 8b ... Switch, 8g ... Switch, 8r ... Switch, 8x ... Operation part, 8y ... Display part, 10 ... Communication part, 12 ... Battery, 22, 52 ... Digital microscope apparatus, 32 ... Light quantity control part, 40 ... Display 50, operation unit, 54 storage unit.

Claims (8)

A rectangular flat plate base material;
LEDs arranged on one surface of the flat plate base material;
An adjustment unit for adjusting the light quantity of the LED disposed on the flat plate base material;
A pathological specimen type optical device comprising a power source for supplying power to the LED.   The pathological specimen optical device according to claim 1, further comprising a communication unit capable of communicating with an external device that remotely operates the adjustment unit.   The pathological specimen optical device according to claim 1 or 2, wherein the LED includes at least one of a red LED, a green LED, a blue LED, and a white LED.   The pathological specimen optical device according to any one of claims 1 to 3, wherein the flat plate base material is formed of a metal member.   The pathological specimen optical device according to any one of claims 1 to 4, wherein a heat sink is formed on the other surface of the flat plate base material.   The pathological specimen device according to any one of claims 1 to 5, wherein the flat plate base material has a size substantially equal to a pathological specimen. A microscope that illuminates and magnifies a pathological specimen with illumination light emitted from a full-color LED,
A microscope comprising: a light amount adjusting unit that adjusts the light amount of each of a red LED, a green LED, and a blue LED constituting the full-color LED based on a color of a staining reagent that stains the pathological specimen. A designation unit for designating the type of the staining reagent;
The light amount adjustment unit stains the illumination light emitted from the full-color LED with the color of the pathological specimen stained with the staining reagent or the staining reagent when the type of the staining reagent is specified by the specifying unit. The microscope according to claim 7, wherein the light quantity of each of the red LED, the green LED, and the blue LED is adjusted to be a complementary color of the color of the pathological specimen.

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