JP2016136852A - Bio-related substance purification cartridge set - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a bio-related substance purification cartridge set for which, when a user uses it, he/she can assemble a plurality of containers.SOLUTION: A bio-related substance purification cartridge set 5a of the invention is attached to a bio-related substance purification device and includes a first container 6 and a second container 7 which is joined to the first container 6. Either of a first fluid and a second fluid is a fluid for purifying a substance. The first container 6 includes a first joint part 227 in which a guiding part 227a is formed. The second container 7 includes: a second joint part 235 in which a guided part 235a guided by the guiding part 227a is formed; and a phase management part 237 which sets an angle around an axis Xb of a second flow passage 2e to the bio-related substance purification device. When the first container 6 and the second container 7 are joined, the guiding part 227a touches the guided part 235a to set an angle around the axis Xb of the first container 6 to the second container 7.SELECTED DRAWING: Figure 11

Description

  The present invention relates to a biological material purification cartridge set.

  In the field of biochemistry, a technique of PCR (Polymerase Chain Reaction) has been established. Recently, the accuracy and detection sensitivity of amplification in the PCR method have improved, and it has become possible to amplify, detect, and analyze a very small amount of sample (DNA, etc.). PCR is a technique for amplifying a target nucleic acid by subjecting a solution (reaction solution) containing a nucleic acid (target nucleic acid) to be amplified and a reagent to thermal cycling. As a thermal cycle of PCR, a method of performing a thermal cycle at two or three stages of temperatures is common.

  On the other hand, the diagnosis of infectious diseases such as influenza in the medical field is currently mainly performed using a simple test kit such as immunochromatography. However, in such a simple test, the accuracy may be insufficient, and it is desired to apply PCR that can be expected to have a higher test accuracy to the diagnosis of infectious diseases.

  In recent years, as a device used in the PCR method or the like, by laminating an aqueous liquid layer and a water-insoluble gel layer alternately in a capillary (in a cartridge) and passing a magnetic particle to which a nucleic acid is attached, A device for purifying a nucleic acid has been proposed (see Patent Document 1). Patent Document 1 describes that alcohol is used as a washing liquid for washing nucleic acid particles with magnetic particles, and water is used as an elution liquid for eluting nucleic acids from magnetic particles.

International Publication No. 2012/086243

  However, the device as described above is composed of an integrated container from the sample supply unit to the PCR reaction solution. For example, when stored in such a device for a long period of time, water contained in a washing solution or an eluate may diffuse and come into contact with a reagent for performing a nucleic acid amplification reaction, thereby inhibiting PCR.

  One of the objects according to some aspects of the present invention is to provide a biomaterial purifying cartridge set in which a plurality of containers can be assembled when used by a user.

[Application Example 1]
The bio-related substance purification cartridge set according to the present invention is:
A biological substance purification cartridge set to be attached to a biological substance purification apparatus,
A first container having a first flow path in which a first fluid is sealed and stored;
A second container having a second flow path in which a second fluid is sealed and accommodated, connected to the first container and communicating with the first flow path;
Including
Either of the first fluid and the second fluid is a fluid for purifying a biological substance,
The first container has a first joint part in which a guide part is formed,
The second container includes a second joint portion in which a guided portion guided by the guide portion is formed, and a phase management portion that sets an angle around the axis of the second flow path with respect to the biological material purification apparatus. Have
When the first container and the second container are joined, the guide part contacts the guided part and sets an angle around the axis of the first container with respect to the second container.

  According to the biological material purification cartridge set according to this application example, the first fluid and the second fluid are sealed and stored in separate containers, thereby suppressing the movement of the fluid between the containers until the containers are joined to each other. can do. In particular, according to the biological material purification cartridge set according to this application example, when the second container for the biological material purification apparatus and the first container are joined, the guide unit and the guided unit are used to connect the second container to the second container. Thus, the first container can be set at a predetermined angle around the axis of the second flow path. Therefore, in the biological material purification cartridge set according to the application example, the biological material purification cartridge, particularly the first container of the biological material purification cartridge joined by the user, is purified at a predetermined angle around the axis of the second flow path. Can be set for the device.

[Application Example 2]
In the biological material purification cartridge set according to the present invention,
The guided portion is formed at one or more locations in the second joint portion,
In the first joint portion, the guide portion may be formed every 90 degrees at four locations around the axis of the first flow path.

  According to the biological material purification cartridge set according to this application example, the second container can be set at an angle of 90 degrees around the axis with respect to the first container, and the burden on the user is small.

[Application Example 3]
In the biological material purification cartridge set according to the present invention,
The guided portion is a protrusion protruding from the outer surface of the second joint portion,
The guide part is a groove formed on the inner surface of the first joint part,
The protrusion may be guided in contact with a side surface of the groove.

  According to the biological substance purification cartridge set according to this application example, the protrusion is brought into contact with the side surface of the groove and guided to guide the second container to the predetermined angle around the axis of the second channel with respect to the first container. Can be set more reliably.

[Application Example 4]
In the biological material purification cartridge set according to the present invention,
The first container includes an adsorption container that forms the first flow path in which an adsorbing liquid is sealed and stored,
The second container includes an elution container that forms the second flow path in which an eluate is sealed and stored,
The adsorbent is a liquid that adsorbs a biological substance on a substance-binding solid phase carrier,
The eluate may be a liquid that elutes a biological substance from the substance-binding solid phase carrier into the eluate.

  According to the biological material purification cartridge set according to this application example, the elution container can be set at a predetermined angle around the axis of the second flow path with respect to the adsorption container.

[Application Example 5]
In the biological material purification cartridge set according to the present invention,
The first container may further include a cleaning container having a third channel that communicates with the first channel and includes a cleaning liquid for cleaning the substance-binding solid phase carrier on which the biological substance is adsorbed.

  According to the biological substance purification cartridge set according to this application example, the elution container can be set at a predetermined angle around the axis of the second flow path with respect to the cleaning container.

[Application Example 6]
In the biological material purification cartridge set according to the present invention,
The adsorption container has a plate-like body extending along the first flow path,
The plate-like body extends from the first flow path into the third flow path when the first flow path and the third flow path communicate with each other, and the first flow path from the third flow path to the first flow path. It may be possible to move the substance-binding solid support along the plate-like body to the channel.

  According to the biological material purification cartridge set according to this application example, the plate-like body provided in the adsorption container can be set to the biological material purification apparatus at a predetermined angle around the axis. Therefore, the substance-binding solid phase carrier can be moved along the plate-like body from the cleaning container to the adsorption container by the operation of the biological substance-purifying device.

[Application Example 7]
In the biological material purification cartridge set according to the present invention,
The guide part is a first guide part provided at one end of the cleaning container,
The adsorption container has a second guide part,
The cleaning container has a second guided portion guided to the second guiding portion at the other end,
The second guide part contacts the second guided part when joining the adsorption container and the cleaning container, and makes an angle around the axis of the third flow path of the cleaning container with respect to the adsorption container. It may be set.

  According to the biological material purification cartridge set according to this application example, the angle around the axis between the cleaning container and the second container can be set, and the angle around the axis between the cleaning container and the adsorption container can be set. Therefore, the angles around the axes of the adsorption container and the cleaning container and the second container can be set.

[Application Example 8]
In the biological material purification cartridge set according to the present invention,
The plate-like body is a first plate-like body,
The cleaning container includes a first cleaning container having the third flow path and a second cleaning container having a fourth flow path,
The first cleaning container has a second plate-like body extending along the third flow path,
The second plate-like body extends from the third flow path into the fourth flow path when the first cleaning container and the second cleaning container are joined, and from the fourth flow path, It may be possible to move the substance-binding solid phase carrier along the second plate-like body to the third channel.

  In the biological material purification cartridge set according to this application example, the second plate-like body provided in the first cleaning container can be set with respect to the biological material purification apparatus at a predetermined angle around the axis. Therefore, the substance-binding solid phase carrier is moved along the first plate and the second plate from the second washing container to the first washing container and from the first washing container to the adsorption container by the operation of the biological substance purification apparatus. Can be moved.

[Application Example 9]
In the biological material purification cartridge set according to the present invention,
The guide part is a first guide part provided at one end of the second cleaning container,
The first cleaning container has a third guide part,
The second cleaning container has a third guided portion guided by the third guide portion at the other end,
The third guide portion is configured to move the third cleaning container when the first cleaning container and the second cleaning container are joined.
The angle around the axis of the fourth flow path of the second cleaning container relative to the first cleaning container may be set in contact with the guided portion.

  According to the biological material purification cartridge set according to this application example, (1) the angle around the axis of the second cleaning container and the second container can be set, and (2) the second cleaning container and the first cleaning container An angle around the axis can be set, and (3) an angle around the axis between the first cleaning container and the adsorption container can be set. Therefore, the angles around the axes of the adsorption container, the first cleaning container, and the second cleaning container and the second container can be set.

[Application Example 10]
In the biological material purification cartridge set according to the present invention,
The second container further includes a third cleaning container having a fifth flow path communicating with the second flow path,
The guided portion is a first guided portion, provided at one end of the third cleaning container,
The third cleaning container has a fourth guide at the other end,
The elution container has a fourth guided portion guided by the fourth guide portion,
The fourth guide part contacts the fourth guided part when the third cleaning container and the elution container are joined, and the shaft of the second flow path of the elution container with respect to the third cleaning container A surrounding angle may be set.

  According to the biological substance purification cartridge set according to this application example, the angle around the axis between the elution container and the third washing container can be set, and the angle around the axis between the third washing container and the first container can be set. it can. Therefore, the angles around the axes of the first container (including the adsorption container), the third cleaning container, and the elution container can be set.

[Application Example 11]
In the biological material purification cartridge set according to the present invention,
The fourth guided portion is a groove provided on the outer surface of the elution container,
The fourth guide portion is a plate-like body extending in the axial direction of the third cleaning container, and is formed so that the width increases from the distal end toward the proximal end,
The width of the base end of the fourth guide part may be the same as the width of the groove of the fourth guided part.

  According to the biological material purification cartridge set according to this application example, when the elution container and the third cleaning container are joined, the proximal end of the fourth guide portion is guided by the groove of the fourth guided portion and the axis is rotated. The angle can be set.

[Application Example 12]
In the biological material purification cartridge set according to the present invention,
The phase management unit may be a plate-like body that extends in a direction parallel to the axis of the second flow path and protrudes from the outer surface of the second container.

  According to the biological substance purification cartridge set according to this application example, the second container is placed at a predetermined angle around the axis with respect to the biological substance purification apparatus by a plate-like body extending in a direction parallel to the axis of the second flow path. Can be set.

[Application Example 13]
In the biological material purification cartridge set according to the present invention,
The phase management unit may be a groove extending in a direction parallel to the axis of the second flow path.

According to the biological substance purification cartridge set according to this application example, the second container can be set at a predetermined angle around the axis with respect to the biological substance purification apparatus by the groove extending in the direction parallel to the axis of the second flow path. .

[Application Example 14]
In the biological material purification cartridge set according to the present invention,
The phase management unit may be a mark provided on the outer surface of the first container.

  According to the biological material purification cartridge set according to this application example, the second container can be set at a predetermined angle around the axis with respect to the biological material purification device by the mark provided on the outer surface.

It is a front view of the container assembly 1 which concerns on embodiment. It is a side view of the container assembly 1 which concerns on embodiment. It is a top view of container assembly 1 concerning an embodiment. It is a perspective view of container assembly 1 concerning an embodiment. It is AA sectional drawing in FIG. 3 of the container assembly 1 which concerns on embodiment. It is CC sectional drawing in FIG. 3 of the container assembly 1 which concerns on embodiment. It is a schematic diagram explaining operation of the container assembly 1 which concerns on embodiment. It is a schematic diagram explaining operation of the container assembly 1 which concerns on embodiment. 1 is a schematic configuration diagram of a PCR device 50. FIG. 2 is a block diagram of a PCR device 50. FIG. It is sectional drawing of the biological material purification cartridge set 5a which concerns on embodiment. FIG. 7 is a development view of the first joint portion 227. FIG. 6 is a development view of a second joint portion 235. FIG. 6 is a side view of the second container 7. It is DD sectional drawing in FIG. 11 of the 1st container 6. FIG. FIG. 4 is a schematic diagram for explaining a moving direction of a magnetic bead 30. It is a schematic diagram explaining the state which fixed the phase management part 237 to the biological material refinement | purification apparatus 51. FIG. FIG. 4 is a schematic diagram for explaining an assembly structure of a first container 6. FIG. 6 is a schematic diagram for explaining an assembly structure of a second container 7. FIG. 10 is a perspective view for explaining a modified example of the third cleaning container 230. FIG. 10 is a perspective view for explaining a modified example of the third cleaning container 230.

  DESCRIPTION OF EMBODIMENTS Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the drawings. The embodiments described below do not unduly limit the contents of the present invention described in the claims. In addition, not all of the configurations described below are essential constituent requirements of the present invention.

  The biological material purification cartridge set according to the present embodiment is a biological material purification cartridge set to be attached to the biological material purification apparatus, and includes a first container having a first flow path in which a first fluid is sealed and housed. A second container having a second flow path in which a second fluid is sealed and accommodated, which is joined to the first container and communicated with the first flow path, and the first fluid and the second fluid Is a fluid for purifying a biological substance, the first container has a guide part, the second container has a guided part guided by the guide part, and the biological substance purification apparatus A phase management unit that sets an angle around the axis of the second flow path with respect to the guide, and the guide unit contacts the guided unit when the first container and the second container are joined together An angle around the axis of the first container relative to the second container. Characterized in that it.

  Here, the biological substance is a substance related to the living body, such as nucleic acid (DNA, RNA), biological polymer such as polypeptide, protein, polysaccharide, protein, enzyme, peptide, nucleotide, amino acid, vitamin, etc. Including low molecular organic compounds derived from living organisms and inorganic compounds. In the following embodiments, a nucleic acid is used as a biological material for explanation.

  The substance-binding solid phase carrier is a substance capable of holding a biological substance by adsorption, that is, by reversible physical bonding. The shape of the substance-binding solid phase carrier is preferably fine particles, but is not limited thereto, and may be fine fibers or nets, and is not particularly limited. The substance-binding solid phase carrier preferably has magnetism in order to move it in a desired direction while adsorbing the biological substance. In the following embodiment, a description will be given using a magnetic bead 30 (see FIGS. 7 and 8 described later) that adsorbs a nucleic acid as a substance-binding solid phase carrier.

  The cleaning liquids 12, 14, and 16 (see FIGS. 7 and 8 to be described later) are liquids for cleaning the substance-binding solid phase carrier on which the biological substance is adsorbed. Therefore, by washing the substance-bound solid phase carrier with the washing liquid, it is possible to remove other impurities while adsorbing the biological substance adsorbed on the substance-bound solid phase carrier more stably.

  The fluid immiscible with the cleaning liquid is a fluid immiscible with the cleaning liquid in the cleaning container and can be phase-separated from the cleaning liquid. The fluid immiscible with the cleaning liquid is a substance inert to the cleaning liquid and includes a gas such as air. As the fluid that is immiscible with the cleaning liquid, when the cleaning liquid is an aqueous liquid, for example, oil or oil gel that is immiscible with the cleaning liquid can be used. The oil gel is obtained by gelling liquid oil with a gelling agent. In the present embodiment, the term “oil” simply excludes gelled ones. In the following embodiments, description will be given using oils 20, 22, 24, and 26 (see FIGS. 7 and 8 described later) as fluids that are immiscible with the cleaning liquid.

  The eluent 32 (see FIG. 7 and FIG. 8 described later) is one for detaching the biological substance from the substance-binding solid phase carrier and eluting it into the eluate. For example, water or a buffer solution can be used as the eluate.

  The fluid that is immiscible with the eluate is a fluid that is immiscible with the eluate in the elution container, and can be phase-separated from the eluate. A fluid that is immiscible with the eluate is a substance that is inert to the eluate. In the following embodiments, description will be given using oil 26 (see FIGS. 7 and 8 described later) as a fluid immiscible with the cleaning liquid.

  The biorelevant substance purification cartridge set according to the present invention is a set for assembling a cartridge for purifying a biorelevant substance. That is, when the biological material purification cartridge set according to the present invention is assembled, a cartridge for purifying the biological material can be obtained. Hereinafter, the cartridge (container assembly) will be described first, and then the biological material purification cartridge set will be described.

1. Outline of Container Assembly First, an outline of the container assembly 1 according to the present embodiment will be described with reference to FIGS. FIG. 1 is a front view of a container assembly 1 (hereinafter sometimes referred to as a cartridge) according to an embodiment. FIG. 2 is a side view of the container assembly 1 according to the embodiment. FIG. 3 is a plan view of the container assembly 1 according to the embodiment. FIG. 4 is a perspective view of the container assembly 1 according to the embodiment. In addition, the state of the container assembly 1 in FIGS. 1 to 3 will be described as an upright state.

The container assembly 1 includes an adsorption container 100, a cleaning container 200, an elution container 300, and a reaction container 400. The container assembly 1 is a container that forms a flow path (not shown) that communicates from the adsorption container 100 to the reaction container 400. The flow path of the container assembly 1 is closed at one end by the cap 110 and at the other end by the bottom 402.

  The container assembly 1 binds a nucleic acid to magnetic beads (not shown) in the adsorption container 100, purifies the magnetic beads while moving in the washing container 200, and extracts the nucleic acid in an eluate droplet (not shown) in the elution container 300. This is a container that performs pre-treatment for elution and thermal cycle processing of polymerase reaction for the droplet of eluate containing nucleic acid in the reaction container 400.

  Although the material of the container assembly 1 is not specifically limited, For example, glass, a polymer | macromolecule, a metal etc. can be used. It is more preferable to select a material having transparency in visible light such as glass or polymer as the material of the container assembly 1 because the inside (inside the cavity) can be observed from the outside of the container assembly 1. In addition, when a material that transmits magnetic force or a non-magnetic material is selected as the material of the container assembly 1, a magnetic force is applied from the outside of the container assembly 1 when a magnetic bead (not shown) is passed through the container assembly 1. Is preferred because it facilitates this. The material of the container assembly 1 can be, for example, polypropylene resin.

  The adsorption container 100 includes a cylindrical syringe part 120 that accommodates an adsorbing liquid (not shown), a plunger part 130 that is a movable pusher inserted into the syringe part 120, and one of the plunger parts 130. And a cap 110 that is fixed to the end of the head. The adsorption container 100 moves the cap 110 with respect to the syringe part 120 to slide the plunger part 130 on the inner surface of the syringe part 120, and pushes an unillustrated adsorbing liquid stored in the syringe part 120 to the cleaning container 200. be able to. The adsorbing liquid will be described later.

  The cleaning container 200 is obtained by joining and assembling the first to third cleaning containers 210, 220, and 230. The first to third cleaning containers 210, 220, and 230 each have one or more cleaning liquid layers partitioned by an oil layer (not shown). Then, by joining the first to third cleaning containers 210, 220, and 230, the cleaning container 200 has a plurality of cleaning liquid layers partitioned by a plurality of oil layers (not shown) inside. In the cleaning container 200 of the present embodiment, the example using the three cleaning containers including the first to third cleaning containers 210, 220, and 230 has been described. However, the present invention is not limited thereto, and the number may be appropriately increased or decreased according to the number of cleaning liquid layers. be able to. The cleaning liquid will be described later.

  The elution container 300 is joined to the third cleaning container 230 of the cleaning container 200 and accommodates the eluate therein so that the shape of the plug can be maintained. Here, the “plug” means a liquid when a specific liquid occupies one section in the flow path. More specifically, the plug of a specific liquid refers to a columnar shape in which only the specific liquid occupies the inside in the longitudinal direction of the flow path, and a certain space inside the flow path is formed by the liquid plug. Shows a state where is marked. The expression “substantially” here means that a small amount (for example, a thin film) of another substance (liquid or the like) may be present around the plug, that is, on the inner wall of the flow path. The eluate will be described later.

  The nucleic acid purification device 5 includes an adsorption container 100, a cleaning container 200, and an elution container 300.

  The reaction container 400 is a container that is joined to the elution container 300 and receives the liquid pushed out from the elution container 300, and is a container that stores droplets of the eluate containing the specimen during the thermal cycle process. Moreover, the reaction container 400 stores a reagent (not shown). The reagent will be described later.

2. Detailed Structure of Container Assembly Next, the detailed structure of the container assembly 1 will be described with reference to FIGS. FIG. 5 is a cross-sectional view taken along the line AA in FIG. 3 of the container assembly 1 according to the embodiment. FIG. 6 is a cross-sectional view taken along the line CC of FIG. 3 of the container assembly 1 according to the embodiment. Actually, the container assembly 1 is assembled in a state in which the contents such as the cleaning liquid are filled. However, in FIGS. 5 and 6, the contents are described to explain the structure of the container assembly 1. Is omitted.

2-1. Adsorption container In the adsorption container 100, the plunger part 130 is inserted from one opening end of the syringe part 120, and the cap 110 is inserted in the opening end of the plunger part 130. The cap 110 has a ventilation portion 112 at the center thereof, and the change in the internal pressure of the plunger portion 130 can be suppressed by the ventilation portion 112 when the plunger portion 130 is operated.

  The plunger portion 130 is a substantially cylindrical pusher that slides on the inner peripheral surface of the syringe portion 120. The plunger portion 130 has an opening end portion into which the cap 110 is inserted and a bottom portion facing the opening end portion of the syringe portion 120. It has the rod-shaped part 132 extended in a longitudinal direction, and the front-end | tip part 134 of the front-end | tip of the rod-shaped part 132. The rod-shaped portion 132 protrudes from the center of the bottom portion of the plunger portion 130, and a through-hole is formed around the rod-shaped portion 132 so that the plunger portion 130 and the syringe portion 120 communicate with each other.

  The syringe part 120 constitutes a part of the flow path 2 of the container assembly 1, and includes a large diameter part that accommodates the plunger part 130, a small diameter part having an inner diameter smaller than the large diameter part, and a large diameter part to a small diameter part. A reduced diameter portion that reduces the inner diameter of the lip, a suction insertion portion 122 at the tip of the small diameter portion, and a cylindrical suction cover portion 126 that covers the periphery of the suction insertion portion 122. The large diameter portion, the small diameter portion, and the suction insertion portion 122 that are part of the flow path 2 of the container assembly 1 are substantially cylindrical.

  The tip part 134 of the plunger part 130 seals the small diameter part of the syringe part 120 and partitions the large diameter part, the reduced diameter part, and the small diameter part at the time of provision to the operator to form two compartments.

  The suction insertion part 122 of the syringe part 120 is inserted and fitted into the first receiving part 214 which is one open end part of the first cleaning container 210 in the cleaning container 200, so that the syringe part 120 and the first cleaning container are fitted. 210 is joined. The outer peripheral surface of the suction insertion portion 122 and the inner peripheral surface of the first receiving portion 214 are in close contact with each other to prevent the liquid as the contents from leaking to the outside.

2-2. Cleaning Container The cleaning container 200 is an assembly that forms part of the flow path 2 of the container assembly 1 and includes the first to third cleaning containers 210, 220, and 230. Since the basic structures of the first to third cleaning containers 210, 220, and 230 are the same, the structure of the first cleaning container 210 will be described, and the description of the second and third cleaning containers 220 and 230 will be omitted. To do.

  The first cleaning container 210 has a substantially cylindrical shape extending in the longitudinal direction of the container assembly 1, and includes a first insertion portion 212 formed at one opening end and a first opening formed at the other opening end. It has a receiving part 214 and a cylindrical first cover part 216 that covers the periphery of the first insertion part 212.

  The outer diameter of the first insertion part 212 is substantially the same as the inner diameter of the second receiving part 224. Further, the inner diameter of the first receiving portion 214 is substantially the same as the outer diameter of the suction insertion portion 122.

By inserting and fitting the first insertion portion 212 of the first cleaning container 210 into the second receiving portion 224 of the second cleaning container 220, the outer periphery of the first insertion portion 212 is aligned with the inner periphery of the second receiving portion 224. The first cleaning container 210 and the second cleaning container 220 are joined together while closely sealing. Similarly, the first to third cleaning containers 210, 220, and 230 are connected to form the cleaning container 200. Here, “sealing” means sealing so that at least liquid or gas contained in a container or the like does not leak to the outside, including sealing that liquid or gas enters from the outside to the inside. Good.

2-3. The elution container The elution container 300 has a substantially cylindrical shape extending in the longitudinal direction of the container assembly 1 and constitutes a part of the flow path 2 of the container assembly 1. The elution container 300 has an elution insertion portion 302 formed at one opening end portion and an elution receiving portion 304 formed at the other opening end portion.

  The inner diameter of the elution receiving part 304 is substantially the same as the outer diameter of the third insertion part 232 of the third cleaning container 230. By inserting and fitting the third insertion portion 232 into the elution receiving portion 304, the outer periphery of the third insertion portion 232 is in close contact with the inner periphery of the elution receiving portion 304 and is sealed with the third cleaning container 230. The container 300 is joined.

2-4. Reaction container The reaction container 400 has a substantially cylindrical shape extending in the longitudinal direction of the container assembly 1 and constitutes a part of the flow path 2 of the container assembly 1. The reaction vessel 400 has a reaction receiving portion 404 formed at the open end, a bottom portion 402 formed at the other closed end, and a reservoir portion 406 that covers the reaction receiving portion 404.

  The inner diameter of the reaction receiving unit 404 is substantially the same as the outer diameter of the elution insertion unit 302 of the elution container 300. The elution container 300 and the reaction container 400 are joined by inserting and fitting the elution insertion part 302 into the reaction receiving part 404.

  A reservoir unit 406 having a predetermined space is provided around the reaction receiving unit 404. The reservoir unit 406 has a volume capable of receiving the liquid overflowing from the reaction vessel 400 due to the movement of the plunger unit 130.

3. Contents of Container Assembly and Operation of Container Assembly Next, the contents of the container assembly 1 will be described with reference to FIG. 7A, and the operation of the container assembly 1 will be described with reference to FIGS. explain. Drawing 7 is a mimetic diagram explaining operation of container assembly 1 concerning an embodiment. Drawing 8 is a mimetic diagram explaining operation of container assembly 1 concerning an embodiment. 7 and 8, each container is represented by a flow path 2 and the outer shape and the joining structure are omitted in order to explain the state of the contents.

3-1. Contents FIG. 7A shows the state of the contents in the flow path 2 in the state of FIG. The contents in the flow path 2 are, in order from the cap 110 toward the reaction vessel 400, the adsorbed liquid 10, the first oil 20, the first cleaning liquid 12, the second oil 22, the second cleaning liquid 14, the third oil 24, Magnetic beads 30, third oil 24, third cleaning liquid 16, fourth oil 26, eluent 32, fourth oil 26, and reagent 34.

In the flow channel 2, portions having a large cross-sectional area (a thick portion of the flow channel 2) and small portions (a thin portion of the flow channel 2) are alternately arranged on a surface orthogonal to the longitudinal direction of the container assembly 1. Part or all of the first to fourth oils 20, 22, 24, 26 and the eluate 32 are accommodated in a narrow portion of the flow path 2. The cross-sectional area of the narrow part of the flow path 2 is maintained stably when the interface of adjacent liquids (which may be fluids; the same applies hereinafter) is disposed in the narrow part of the flow path 2. Has a possible area. Therefore, the liquid disposed in the narrow portion of the flow path 2 can stably maintain the positional relationship between the liquid and the other liquid disposed above and below the liquid. Even if the interface between the liquid disposed in the narrow portion of the flow path 2 and the other liquid disposed in the thick portion of the flow path 2 is formed in the thick portion of the flow path 2, Even if the interface is disturbed, the interface is stably formed at a predetermined position by placing the interface in a stationary state.

  The narrow portion of the flow path 2 is formed inside the adsorption insertion portion 122, the first insertion portion 212, the second insertion portion 222, the third insertion portion 232, and the elution insertion portion 302. In the elution container 300, the elution insertion portion 302 is formed. Extends upward beyond. In addition, the liquid accommodated in the thin part of the flow path 2 is stably maintained even before the container is assembled.

3-1-1. Oil The first to fourth oils 20, 22, 24, and 26 are all made of oil, and exist as plugs between liquids before and after each oil in the state of FIG. Since the first to fourth oils 20, 22, 24, and 26 exist as plugs, the liquids adjacent to each other before and after each oil are selected as liquids that are phase-separated from each other, that is, liquids that are not miscible. The oils constituting the first to fourth oils 20, 22, 24, 26 may be different types of oils. Examples of the oil that can be used for these include one selected from silicone oil such as dimethyl silicone oil, paraffin oil, mineral oil, and mixtures thereof.

3-1-2. Adsorbing liquid The adsorbing liquid 10 refers to a liquid that serves as a field for adsorbing nucleic acids to the magnetic beads 30 and is, for example, an aqueous solution containing a chaotropic substance. As the adsorbing liquid 10, for example, 5M guanidine thiocyanate, 2% Triton X-100, 50 mM Tris-HCl (pH 7.2) can be used. The adsorbing liquid 10 is not particularly limited as long as it contains a chaotropic substance, but the adsorbing liquid 10 may contain a surfactant for the purpose of disrupting the cell membrane or denaturing proteins contained in the cells. The surfactant is not particularly limited as long as it is generally used for nucleic acid extraction from cells or the like. Specifically, a Triton surfactant such as Triton-X or a tween surfactant such as Tween 20 is used. Nonionic surfactants such as agents, and anionic surfactants such as sodium N-uraroyl sarcosine (SDS) can be mentioned, with nonionic surfactants in particular in the range of 0.1 to 2% It is preferable to use so that it becomes. Furthermore, it is preferable to contain a reducing agent such as 2-mercaptoethanol or dithiothreitol. The lysis solution may be a buffer solution, but is preferably neutral at pH 6-8. Taking these into account, specifically, 3M-7M guanidine salt, 0% -5% nonionic surfactant, 0 mM-0.2 mM EDTA, 0M-0.2M reducing agent, etc. It is preferable to contain.

  Here, the chaotropic substance has a function of generating chaotropic ions (a monovalent anion having a large ionic radius) in an aqueous solution and increasing the water solubility of the hydrophobic molecule. If it contributes to adsorption | suction, it will not specifically limit. Specific examples include guanidine hydrochloride, sodium iodide, sodium perchlorate, and the like. Among these, guanidine thiocyanate or guanidine hydrochloride having a strong protein-modifying action is preferable. The specified concentration of these chaotropic substances varies depending on each substance. For example, when guanidine thiocyanate is used, it is within a range of 3M to 5.5M, and when guanidine hydrochloride is used, it is used at 5M or more. Is preferred.

  Since the chaotropic substance is present in the aqueous solution, the nucleic acid in the aqueous solution is thermodynamically advantageous when adsorbed on a solid rather than surrounded by water molecules. Will be adsorbed.

3-1-3. Cleaning Liquid The first to third cleaning liquids 12, 14, and 16 are for cleaning the magnetic beads 30 to which the nucleic acid is bound.

  The first cleaning liquid 12 is a liquid that is phase-separated from both the first oil 20 and the second oil 22. The first washing liquid 12 is preferably water or a low salt concentration aqueous solution, and in the case of a low salt concentration aqueous solution, it is preferably a buffer solution. The salt concentration of the low salt concentration aqueous solution is preferably 100 mM or less, more preferably 50 mM or less, and most preferably 10 mM or less. Moreover, the 1st washing | cleaning liquid 12 may contain surfactant as mentioned above, and pH is not specifically limited. Although the salt for using the 1st washing | cleaning liquid 12 as a buffer solution is not specifically limited, Salts, such as a tris, hepes, pipes, and phosphoric acid, are preferable. Furthermore, the first washing solution 12 preferably contains alcohol in an amount that does not inhibit the adsorption of the nucleic acid to the carrier, the reverse transcription reaction, the PCR reaction, or the like. In this case, the alcohol concentration is not particularly limited.

  The first cleaning liquid 12 may contain a chaotropic substance. For example, when guanidine hydrochloride is contained in the first washing liquid 12, the magnetic beads 30 and the like can be washed while maintaining or enhancing the adsorption of the nucleic acid adsorbed to the magnetic beads 30 and the like.

  The second cleaning liquid 14 is a liquid that is phase-separated from both the second oil 22 and the third oil 24. The second cleaning liquid 14 may basically have the same or different composition as the first cleaning liquid 12, but is preferably a solution that does not substantially contain a chaotropic substance. This is to eliminate the introduction of chaotropic substances into the later solution. As the 2nd washing | cleaning liquid 14, you may consist of 5 mM Tris hydrochloric acid buffer, for example. As described above, the second cleaning liquid 14 preferably contains alcohol.

  The third cleaning liquid 16 is a liquid that is phase-separated from both the third oil 24 and the fourth oil 26. The third cleaning liquid 16 may basically have the same or different composition as the second cleaning liquid 14, but does not contain alcohol. The third cleaning liquid 16 may include citric acid to prevent alcohol from being brought into the reaction vessel 400.

3-1-4. Magnetic Beads The magnetic beads 30 are beads that adsorb nucleic acids and preferably have relatively strong magnetism so that they can be moved by the magnet 3 outside the container assembly 1. The magnetic beads 30 may be, for example, silica beads or silica-coated beads. The magnetic beads 30 may be preferably silica-coated beads.

3-1-5. The eluate 32 is a liquid that is phase-separated from the fourth oil 26, and exists as a plug sandwiched between the fourth oils 26 and 26 in the flow path 2 in the elution container 300. The eluate 32 is a liquid for eluting the nucleic acid adsorbed on the magnetic beads 30 from the magnetic beads 30 into the eluate 32. Further, the eluate 32 becomes droplets in the fourth oil 26 by heating. For the eluent 32, for example, pure water can be used. Here, the “droplet” is a liquid surrounded by a free surface.

3-1-6. Reagent Reagent 34 contains components necessary for the reaction. When the reaction in the reaction vessel 400 is PCR, the reagent 34 is an enzyme such as a DNA polymerase for amplifying the target nucleic acid (DNA) eluted in the droplet 36 (see FIG. 8) of the eluate, and At least one of a primer (nucleic acid) and a fluorescent probe for detecting an amplification product can be included, and here, all of the primer, enzyme, and fluorescent probe are included. The reagent 34 is incompatible with the fourth oil 26 and dissolves and reacts when it comes into contact with the droplet 36 of the eluate 32 containing nucleic acid, and is the lowest part in the gravity direction of the flow path 2 in the reaction container 400. Exists in a solid state in the region. For example, the reagent 34 may be freeze-dried (freeze-dried).

3-2. Operation of Container Assembly An example of the operation of the container assembly 1 will be described with reference to FIGS.

The operation of the container assembly 1 is as follows:
(A) a step of assembling the container assembly 1 by joining the adsorption container 100, the cleaning container 200, the elution container 300, and the reaction container 400;
(B) introducing a sample containing nucleic acid into the adsorption container 100 in which the adsorbing liquid 10 is stored;
(C) a step of moving the magnetic beads 30 from the second cleaning container 220 to the adsorption container 100;
(D) swinging the adsorption container 100 to adsorb the nucleic acid to the magnetic beads 30;
(E) From the adsorption container 100, the first oil 20, the first cleaning liquid 12, the second oil 22, the second cleaning liquid 14, the third oil 24, the third cleaning liquid 16, and the fourth oil 26 are passed in this order, and the elution container Moving the magnetic beads 30 adsorbed with nucleic acids to 300;
(F) a step of eluting the nucleic acid from the magnetic beads 30 with respect to the eluate 32 in the elution container 300;
(G) contacting the droplet containing the nucleic acid with the reagent 34 in the reaction vessel 400;
including.

  Hereinafter, each process is demonstrated in order.

(A) Step of assembling container assembly 1 As shown in FIG. 7A, the step of assembling is a flow path that joins from the adsorption vessel 100 to the reaction vessel 400 and continues from the adsorption vessel 100 to the reaction vessel 400. Assemble container assembly 1 to form 2. In FIG. 7A, the cap 110 is attached to the adsorption container 100, but the cap 110 is attached to the plunger portion 130 after the step (B).

  More specifically, the elution insertion section 302 of the elution container 300 is inserted into the reaction receiving section 404 of the reaction container 400, and the third insertion section 232 of the third cleaning container 230 is inserted into the elution receiving section 304 of the elution container 300. The second insertion part 222 of the second cleaning container 220 is inserted into the third receiving part 234 of the third cleaning container 230, and the first insertion part of the first cleaning container 210 is inserted into the second receiving part 224 of the second cleaning container 220. 212 is inserted, and the suction insertion part 122 of the suction container 100 is inserted into the first receiving part 214 of the first cleaning container 210.

(B) Step of introducing the sample In the step of introducing, for example, a cotton swab to which the sample is attached is inserted into the adsorbing liquid 10 from the opening where the cap 110 of the adsorption container 100 is attached, and this is immersed in the adsorbing liquid 10. Do. More specifically, a cotton swab is inserted from an opening at one end of the plunger portion 130 in a state of being inserted into the syringe portion 120 of the adsorption container 100. Next, the cotton swab is taken out from the adsorption container 100 and the cap 110 is attached. This is the state shown in FIG. Further, the specimen may be introduced into the adsorption container 100 by a pipette or the like. In addition, if the specimen is in a paste form or a solid form, the specimen may be attached to or put into the inner wall of the plunger unit 130 with a scissors or tweezers, for example. As shown in FIG. 7A, the adsorbing liquid 10 is partially filled in the syringe part 120 and the plunger part 130, but a space is left on the opening side where the cap 110 is attached. .

The sample contains the target nucleic acid. Hereinafter, this may be simply referred to as a target nucleic acid. The target nucleic acid is, for example, DNA or RNA (DNA: Deoxyribonucleic Acid and / or RNA: Ribonucleic Acid). The target nucleic acid is extracted from the specimen, eluted in an eluate 32 described later, and then used as a PCR template, for example. Examples of the specimen include blood, nasal mucus, oral mucosa, and other various biological samples.

(C) Step of moving the magnetic beads The step of moving the magnetic beads 30 is a magnet that is sandwiched between the third oils 24 and 24 of the second cleaning container 220 and exists in a plug shape as shown in FIG. The bead 30 is performed by moving the magnet 3 toward the adsorption container 100 in a state where the magnetic force of the magnet 3 arranged outside the container is applied.

  The cap 110 and the plunger part 130 are moved in the direction of extracting from the syringe part 120 in accordance with the movement of the magnetic beads 30 or earlier, and the sample in the adsorbed liquid 10 is moved from the plunger part 130 to the syringe part 120. Move in. Due to the movement of the plunger part 130, the flow path 2 that has been blocked by the tip part 134 communicates with the adsorbing liquid 10.

  The magnetic beads 30 ascend in the flow path 2 as the magnet 3 moves, and reach the adsorbing liquid 10 with the specimen as shown in FIG.

(D) Step of adsorbing nucleic acid to magnetic beads The step of adsorbing nucleic acid is performed by swinging the adsorption container 100. This step can be efficiently performed because the opening of the adsorption container 100 is sealed by the cap 110 so that the adsorbed liquid 10 does not leak out. By this step, the target nucleic acid is adsorbed on the surface of the magnetic bead 30 by the action of the chaotropic agent. In this step, nucleic acids and proteins other than the target nucleic acid may be adsorbed on the surface of the magnetic beads 30.

  As a method of swinging the adsorption container 100, a known device such as a vortex shaker may be used, or shaking may be performed by an operator's hand. Further, the magnetism of the magnetic beads 30 may be used to swing the adsorption container 100 while applying a magnetic field from the outside.

(E) Step of moving magnetic beads adsorbed with nucleic acid The step of moving magnetic beads 30 adsorbed with nucleic acid moves while applying the magnetic force of the magnet 3 from the outside of the adsorption vessel 100, the washing vessel 200, and the elution vessel 300. As a result, the magnetic beads 30 are moved in the adsorbing liquid 10, the first to fourth oils 20, 22, 24, 26 and the first to third cleaning liquids 12, 14, 16.

  For example, a permanent magnet or an electromagnet can be used as the magnet 3. Moreover, the magnet 3 may be moved by an operator's hand or may be performed using a mechanical device or the like. Since the magnetic beads 30 have a property of being attracted by a magnetic force, the relative arrangement of the magnets 3 is changed to the adsorption vessel 100, the cleaning vessel 200, and the elution vessel 300 by using this property. The inside of the flow path 2 is moved. The speed at which the magnetic beads 30 pass through each cleaning liquid is not particularly limited, and the magnetic beads 30 may be moved so as to reciprocate along the longitudinal direction of the flow path 2 in the same cleaning liquid. In addition, when moving particles other than the magnetic bead 30 in a tube, this can be performed using gravity or a potential difference, for example.

(F) Step of Eluting Nucleic Acid In the step of eluting nucleic acid, the nucleic acid is eluted from the magnetic beads 30 in the elution liquid droplets 36 in the elution container 300. The eluate 32 in FIG. 7 was present as a plug in the narrow portion of the flow path of the elution container 300. However, the content liquid can be obtained by heating the reaction container 400 while moving the magnetic beads 30 as described above. It expands and moves upward in the elution container 300 as a droplet 36 as shown in FIG. Then, as shown in FIG. 8A, when the magnetic beads 30 reach the eluate droplets 36 in the elution container 300, the target nucleic acid adsorbed on the magnetic beads 30 is dissolved into the eluate by the action of the eluate. Elution into the liquid droplet 36.

(G) The process of making it contact with the reagent 34 The process of making it contact with the reagent 34 makes the droplet 36 containing a nucleic acid contact the reagent 34 in the lowest part in the reaction container 400. FIG. Specifically, as shown in FIG. 8B, the magnetic beads 30 to which the magnetic force of the magnet 3 is applied by pressing the cap 110 and pressing down the first oil 20 by the tip portion 134 of the plunger portion 130. Is maintained at a predetermined position, the droplet 36 of the eluate from which the target nucleic acid is eluted moves to the reaction vessel 400 and contacts the reagent 34 at the bottom of the reaction vessel 400. The reagent 34 in contact with the droplet 36 melts and mixes with the target nucleic acid in the eluate, and for example, PCR using thermal cycling can be performed.

4). PCR Device A PCR device 50 that performs nucleic acid elution processing and PCR using the container assembly 1 will be described with reference to FIGS. 9 and 10. FIG. 9 is a schematic configuration diagram of the PCR device 50. FIG. 10 is a block diagram of the PCR device 50.

  The PCR device 50 includes a rotation mechanism 60, a magnet moving mechanism 70, a pressing mechanism 80, a fluorescence measuring instrument 55, and a controller 90.

4-1. Rotation mechanism The rotation mechanism 60 includes a rotation motor 66 and a heater 65, and rotates the container assembly 1 and the heater 65 by driving the rotation motor 66. When the rotating mechanism 60 rotates the container assembly 1 and the heater 65 to turn upside down, the droplet containing the target nucleic acid moves in the flow path of the reaction container 400, and thermal cycle processing is performed.

  The heater 65 includes a plurality of heaters (not shown), and can include elution, high temperature, and low temperature heaters, for example. The elution heater heats the plug-like eluate of the container assembly 1 and promotes elution of the target nucleic acid from the magnetic beads to the eluate. The high temperature heater heats the liquid upstream of the flow path of the reaction vessel 400 to a temperature higher than that of the low temperature heater. The low temperature heater heats the bottom 402 of the flow path of the reaction vessel. A temperature gradient can be formed in the liquid in the flow path of the reaction vessel 400 by the high temperature heater and the low temperature heater. The heater 65 is provided with a temperature control device, and the liquid in the container assembly 1 can be set to a temperature suitable for processing in accordance with a command from the controller 90.

  The heater 65 has an opening through which the outer wall of the bottom 402 of the reaction vessel 400 is exposed. The fluorescence measuring device 55 measures the luminance of the droplet of the eluate from the opening.

4-2. Magnet moving mechanism The magnet moving mechanism 70 is a mechanism for moving the magnet 3. The magnet moving mechanism 70 draws the magnetic beads in the container assembly 1 toward the magnet 3 and moves the magnetic beads in the container assembly 1 by moving the magnet 3. The magnet moving mechanism 70 includes a pair of magnets 3, an elevating mechanism, and a swing mechanism.

The swing mechanism is a mechanism that swings the pair of magnets 3 in the left-right direction in FIG. 9 (may be the front-rear direction in FIG. 9). The pair of magnets 3 is disposed so as to sandwich the container assembly 1 mounted on the PCR device 50 from the left and right directions (see FIGS. 7 and 8), and is perpendicular to the flow path of the container assembly 1 (here, The distance between the magnetic beads and the magnet 3 can be made closer in the left-right direction in FIG. Therefore, when the pair of magnets 3 are swung in the left-right direction as indicated by arrows, the magnetic beads in the container assembly 1 move in the left-right direction in accordance with the movement. The elevating mechanism can move the magnet 3 in the vertical direction and move the magnetic beads in the vertical direction in FIG. 9 in accordance with the movement of the magnet 3.

4-3. Pressing mechanism The pressing mechanism 80 is a mechanism that presses the plunger portion of the container assembly 1. When the plunger portion is pressed by the pressing mechanism 80, the droplet in the elution container 300 is pushed into the reaction container 400, PCR can be performed in the reaction vessel 400.

  In FIG. 9, the pressing mechanism 80 is shown arranged above the upright container assembly 1, but the direction in which the pressing mechanism 80 pushes the plunger portion is not the vertical direction in FIG. 9, for example, the vertical direction It may be inclined 45 degrees with respect to. By doing in this way, it becomes easy to arrange | position the press mechanism 80 in the position which does not interfere with the magnet moving mechanism 70. FIG.

4-4. Fluorescence measuring instrument The fluorescence measuring instrument 55 is a measuring instrument that measures the brightness of the droplets in the reaction vessel 400. The fluorescence measuring device 55 is disposed at a position facing the bottom portion 402 of the reaction vessel 400. Note that the fluorescence measuring device 55 is preferably capable of detecting luminance in a plurality of wavelength regions so as to be compatible with multiplex PCR.

4-5. Controller The controller 90 is a control unit that controls the PCR apparatus 50. The controller 90 includes a processor such as a CPU and a storage device such as a ROM and a RAM. Various programs and data are stored in the storage device. Further, the storage device provides an area for developing a program. Various processes are realized by the processor executing the program stored in the storage device.

  For example, the controller 90 controls the rotation motor 66 to rotate the container assembly 1 to a predetermined rotation position. The rotation mechanism 60 is provided with a rotation position sensor (not shown), and the controller 90 drives and stops the rotation motor 66 according to the detection result of the rotation position sensor.

  Further, the controller 90 controls the heater 65 to turn on / off the heater to generate heat, thereby heating the liquid in the container assembly 1 to a predetermined temperature.

  Further, the controller 90 controls the magnet moving mechanism 70 to move the magnet 3 in the vertical direction, and swings the magnet 3 in the horizontal direction in FIG. 9 according to the detection result of a position sensor (not shown).

  In addition, the controller 90 controls the fluorescence measuring device 55 to measure the luminance of the droplet in the reaction container 400. This measurement result is stored in a storage device (not shown) of the controller 90.

  The container assembly 1 is attached to the PCR device 50, and the above steps 3-2 (C) to (G) can be performed, and further PCR can be performed.

5. Biorelevant Substance Purification Cartridge Set A biorelevant substance purification cartridge set 5a according to this embodiment will be described with reference to the drawings. FIG. 11 is a cross-sectional view schematically showing the biological material purification cartridge set 5a according to this embodiment. The biological material purification cartridge set 5a is a part of the container assembly 1 described with reference to FIGS.

  The above-described cartridge (container assembly 1) can be obtained by assembling the biological substance purification cartridge set 5a and further joining a reaction container (not shown) (see FIG. 6). If the reaction container (400) is joined to the biological substance purification cartridge set 5a to form the container assembly 1, for example, it can be used as a nucleic acid amplification reaction cartridge. If the reaction container (400) is not joined, the biological substance It can be used as a purification cartridge.

  As shown in FIG. 11, the biological material purification cartridge set 5 a includes a first container 6 and a second container 7.

  The first container 6 has a first flow path 2a in which a first fluid is sealed and stored. The second container has a second flow path 2e that is joined to the first container 6 and communicates with the first flow path 2a and in which the second fluid is sealed and accommodated.

  Either the first fluid or the second fluid is a fluid for purifying the biological material. As the fluid for purifying the biological substance, the adsorbing liquid 10, the first to third cleaning liquids 12, 14, 16, the eluent 32, etc. described in “3-1. Contents” can be used.

5-1. First Container The first container 6 includes an adsorption container 100 that forms a first flow path 2a in which the adsorbing liquid 10 is sealed and accommodated. The adsorption container 100 is the container described in “2-1. Adsorption container” above. Here, the adsorption container 100 is not equipped with a cap 110 (see FIG. 6). The adsorbing liquid 10 may be a liquid that adsorbs a biological substance on a substance-binding solid phase carrier. The adsorbing liquid 10 may be, for example, the liquid described in “3-1-2. Adsorbing liquid”. In addition to the adsorbent 10, the first flow path 2 a may include the first oil 20 and the air 11 described in “3-1. Contents”.

  The first container 6 includes a cleaning container 200a having a third flow path 2b that communicates with the first flow path 2a and includes a first cleaning liquid 12 that cleans the substance-binding solid phase carrier on which the biological substance is adsorbed. More specifically, the cleaning container 200a includes a first cleaning container 210 having a third flow path 2b containing the first cleaning liquid 12, a second cleaning container 220 having a fourth flow path 2c containing the second cleaning liquid 14, and including. The first cleaning container 210 and the second cleaning container 220 are the containers described in “2-2. Cleaning container”. For example, the first cleaning liquid 12 and the second cleaning liquid 14 may be those described in “3-1-3. Cleaning liquid” above. In addition to the first cleaning liquid 12 and the second cleaning liquid 14, the third flow path 3b and the fourth flow path 2c include the first oil 20, the second oil 22, and the third flow described in "3-1. Contents" above. Oil 24 may be included. The fourth flow path 2c may include the magnetic beads 30 described in the above “3-1-4. Magnetic beads” as the substance-binding solid phase carrier.

  In the first container 6, the adsorption container 100 and the cleaning container 200a are joined, and the first flow path 2a, the third flow path 2b, and the fourth flow path 2c communicate with each other to form one flow path. One channel that is in communication may be used as the first channel. Both ends of the first container 6 are sealed with a film 120c and a film 222c, which are sealing members, and a predetermined fluid is sealed and stored in the first, third, and fourth flow paths 2a, 2b, and 2c. The structure in which the adsorption container 100 and the cleaning container 200 a in the first container 6 are joined is the same as the adsorption container 100, the first cleaning container 210, and the second cleaning container 220 described in “2. Detailed structure of the container assembly”. It is basically the same as the joined structure.

The first cleaning container 210 and the second cleaning container 220 are joined to the adsorption container 100 at a predetermined angle around the axis Xa of the first flow path 2a. The axis Xa is the central axis of the first flow path 2a, and here is the same as the central axes of the third flow path 2b and the fourth flow path 2c.
In addition, the axis Xa extends along the longitudinal direction of the first container 6 and is the central axis when the first container 6 has a substantially cylindrical shape.

  The film 120c is affixed to the surface of the flange part 120b formed in one end part of the syringe part 120 which comprises the adsorption container 100 by heat welding. When the film 120c is peeled off from the flange part 120b, an opening of the plunger part 130 inserted into the syringe part 120 is formed, and the cap 110 can be received in the opening.

  The film 222c is affixed near one end of the fourth flow path 2c of the second cleaning container 220. The second cleaning container 220 has a second cover part 226, the upper end of the second cover part 226 is connected to the outer surface of the second insertion part 222, and the lower end extends beyond the second insertion part 222. The inner surface 226a of the second cover portion 226 has an annular step portion 226b that increases in diameter downward. The step portion 226b is slightly below the lower end of the second insertion portion 222, and a film 222c is attached to the surface thereof by thermal welding.

  In this document, “upper” and “lower” are upper and lower in the drawings unless otherwise specified, and an upstream side where a biological substance (nucleic acid) moves is defined as an upper side, and a downstream side is defined as a lower side.

  In the above example, the first container 6 includes the adsorption container 100 and the cleaning container 200a. However, the present invention is not limited to this, and the first container 6 may include only the adsorption container 100 or the second cleaning container 220. May be.

5-2. Second Container The second container 7 includes an elution container 300 that forms a second flow path 2e in which the eluate 32 is sealed and accommodated. The elution container 300 is the same as that described in “2-3. Elution container”. The eluate 32 may be a liquid that elutes a biological substance from the substance-binding solid phase carrier into the eluate 32. The eluent 32 may be, for example, the liquid described in the above “3-1-5. Eluent”. In addition to the eluate 32, the second flow path 2e may include the fourth oil 26 described in “3-1. Contents”.

  The second container 7 includes a third cleaning container 230 having a fifth flow path 2d communicating with the second flow path 2e. The third cleaning container 230 is the same as that described in “2-2. Cleaning container”. The third cleaning container 230 has a phase management unit 237. The fifth flow path 2d may include the third cleaning liquid 16, the third oil 24, and the fourth oil 26 described in “3-1. Contents”.

  In the second container 7, the third cleaning container 230 and the elution container 300 are joined, and the fifth flow path 2d and the second flow path 2b communicate with each other to form one flow path. One channel connected in this way may be used as the second channel. Both ends of the second container 7 are sealed with a film 230c and a film 306c, which are sealing members, and a predetermined fluid is sealed and accommodated in the fifth and second flow paths 2d and 2e. The structure in which the third cleaning container 230 and the elution container 300 in the second container 7 are joined is basically the same as that described in “2. Detailed structure of the container assembly”.

  The film 230c is attached to the surface of one end portion of the third cleaning container 230 by heat welding. The film 306c is attached to one end of the elution container 300 by heat welding.

  The elution container 300 is joined to the third cleaning container 230 at a predetermined angle around the axis Xb of the second flow path 2e. The axis Xb is the central axis of the second flow path 2e and is the same as the central axis of the fifth flow path 2d. In addition, the axis Xb extends along the longitudinal direction of the second container 7 and is the central axis when the second container 7 has a substantially cylindrical shape.

  In the above example, the example including the third cleaning container 230 and the elution container 300 has been described. However, the present invention is not limited thereto, and only the elution container 300 or only the third cleaning container 230 may be used. Good.

5-3. Joining Structure A joining structure between the first container 6 and the second container 7 will be described with reference to FIGS. FIG. 12 is a development view of the first joint portion 227, and FIG. 13 is a development view of the second joint portion 235. FIG. 14 is a side view of the second container 7.

5-3-1. 1st junction part As shown in Drawing 11, the 1st container 6 has the 1st junction part 227 in which the 1st guide part 227a was formed. The first joint portion 227 is formed at the lower end of the first container 6, that is, the lower end of the second cover portion 226 of the second cleaning container 220. The second cover portion 226 has a cylindrical shape that opens downward, and has an annular inner surface 226a. The second cover part 226 is formed to have a size for receiving the portion of the third cleaning container 230 above the phase management part 237.

  The first joint portion 227 includes a first guide portion 227a, a first hole 227b, and a first vertical groove 227c.

  The first guide portion 227a is a groove formed on the inner surface 226a of the first joint portion 227. The first guide part 227a is formed so that the width gradually decreases upward from the opening end of the second cover part 226. As shown in FIG. 12, the 1st guide part 227a is a groove | channel recessed from the inner surface 226a, and the 1st guided part 235a mentioned later contacts the side walls 227aa and 227ab of the groove | channel. When the first guide portion 227a is unfolded, the two side walls 227aa and 227ab extending from the apex toward the opening end become equal sides of an isosceles triangle. The side walls 227aa and 227ab may be straight or curved when deployed. The side walls 227aa and 227ab only have to be able to guide the first guided portion 235a to the apexes thereof. The 1st guide part 227a is formed every 90 degree | times in four places around the axis | shaft Xa of the 1st flow path 2a. In FIG. 12, the groove region is indicated by hatching.

  The first hole 227b passes through the second cover portion 226 from the inner surface 226a to the outer surface. The first hole 227b is above the apex where the two side walls 227aa and 227ab are connected. There are two first holes 227b, which are at positions facing each other across the axis Xa. The first hole 227b is rectangular, the height in the vertical direction is the same as the height of the first guided portion 235a, and the width in the direction orthogonal to the vertical direction is the same as the width of the first guided portion 235a. .

  The first vertical groove 227c is a groove formed on the surface of the inner surface 226a, and extends upward from the opening end of the second cover portion 226 in a direction parallel to the axis Xa. The first vertical groove 227c is formed to be deeper than the first guide portion 227a. The width of the first vertical groove 227c is the same as the width of a first guided portion 235a described later.

5-3-2. Second Joint Part As shown in FIGS. 11, 13 and 14, the second container 7 has a second joint part 235 in which a first guided part 235a guided by the first guide part 227a is formed.

  The 2nd junction part 235 has the 1st guided part 235a and the 1st guided part 235b. The outer surface of the second joint portion 235 is a substantially cylindrical side surface with the axis Xb as the center.

The first guided portion 235a is a protrusion that protrudes from the outer surface of the second joint portion 235, and protrudes from the upper end of the second joint portion 235 in a direction orthogonal to the axis Xb. As shown in FIG. 12, the first guided portion 235a is a substantially rectangular parallelepiped, and the outer shape of at least the base end portion is rectangular in plan view. The protrusion of the first guided portion 235a is guided by contacting the side walls 227aa and 227ab of the groove of the first guide portion 227a.

  Thus, by guiding the protrusion in contact with the side surface of the groove, the second container 7 can be reliably set with respect to the first container 6 at a predetermined angle around the axis Xb of the second flow path 2e. .

  Below the first guided portion 235a, two first guided portions 235b are arranged at a position facing each other across the axis Xb. The first guided portion 235b is guided to the first guiding portion 227a in the same manner as the first guided portion 235a. The first guided portion 235b is a protrusion that protrudes from the outer surface of the second joint portion 235, and is formed in a substantially isosceles triangle as shown in the developed view, and the side walls 235ba and 235bb that are the same sides of the first guided portion 235b of the first guide portion 227a. It is guided in contact with the side walls 227aa and 227ab of the groove.

  As for the 2nd junction part 235, the 1st guided part 235a is formed in one or more places. If the 1st guide part 227a is equally arrange | positioned at four places as mentioned above, when joining the 2nd container 7 to the 1st container 6, the 2nd container 7 is set to the axis Xb with respect to the 1st container 6. It can be set to an angle of every 90 degrees around. Therefore, the burden on the user of carefully checking whether or not the first container 6 and the second container 7 are joined in a phase around a predetermined axis is small.

  The second joint 235 and the phase manager 237 are always in a fixed relationship. That is, the angle around the axis xb of the second joint part 235 and the angle around the axis Xb of the phase management part 237 always coincide. Here, the first guided portion 235a and the phase management portion 237 are in the same plane including the axis Xb. In this embodiment, since the second joint 235 and the phase management unit 237 are provided in the third cleaning container 230, they may be manufactured in a predetermined positional relationship at the manufacturing stage of the third cleaning container 230. For example, the above-described relationship is also obtained when the second joint 235 is provided in the third cleaning container 230 and the phase management unit 237 is provided in the elution container 300.

5-3-3. Joining Operation A joining operation when joining the second container 7 to the first container 6 will be described.

  As shown by an arrow in FIG. 11, the third receiving part 234 at the end of the second container 7 is inserted into the opening of the second cover part 226 of the first container 6. Since the plurality of flanges of the second container 7 are in contact with the inner surface 226a of the second cover portion 226 of the first container 6 and are aligned, the process is performed so that the axis Xa and the axis Xb are on the same line.

  The third receiving part 234 breaks through the film 222c, and the second insertion part 222 breaks through the film 230c, and the second flow path 2c and the fifth flow path 2e communicate with each other. That is, the first flow path 2a and the second flow path 2e are communicated. At this time, the third oil 24 flows out between the second cover portion 226 and the outer surface of the third cleaning container 230, but flows out of the biological material purification cartridge by the plurality of flanges on the outer surface of the third cleaning container 230. To prevent that.

  The first guided portion 235a receives a propulsive force along the direction of the axis Xb and contacts the first guide portion 227a, thereby generating a rotational force about the axis Xb in the first guided portion 235a. As a result, when the first container 227a joins the first container 6 and the second container 7, the first guide part 227a contacts the first guided part 235a and moves around the axis Xb of the first container 6 relative to the second container 7. The angle will be set.

The two first guided portions 235a guided to the apex of the first guiding portion 227a shown in FIG.
Due to the elastic deformation of the container itself, it gets over the groove of the first guide part 227a and fits into the first hole 227b. In addition, the remaining two first guided portions 235a at the position of the first vertical groove 227c are guided by the first vertical groove 227c and proceed along a direction parallel to the axis Xa. Even if there is no first guided portion 235a, if at least one first guide portion 235b having the same shape as the first guide portion 227a is formed, the axis Xb of the first container 6 relative to the second container 7 The surrounding angle can be set.

  According to the biological material purification cartridge set 5a, the first fluid and the second fluid are sealed and housed in separate containers, so that the movement of the fluid between the containers can be suppressed until the containers are joined. . In particular, when the second container 7 attached to the biological material purifying apparatus and the first container 6 are joined, the second container 7 is connected to the first container 6 by the first guide part 227a and the first guided part 235a. The container 7 can be set to a predetermined angle around the axis Xb of the second flow path 2e.

  Therefore, when the user joins the first container 6 and the second container 7, the first container 6 is set with respect to the biological material purifier at a predetermined angle around the axis Xb of the second flow path 2e. Can do. In other words, the elution container 300 can be set at a predetermined angle around the axis Xb with respect to the adsorption container 100, and the elution container 300 can be set at a predetermined angle around the axis Xb with respect to the cleaning container 200a. it can.

  The angle around the axis Xa or the axis Xb between the containers can be rephrased as an angle around the axis of the flow path at least in a portion where the flow paths of the containers are connected to each other. Further, the guided portion and the guiding portion are divided for convenience, and either one may be guided. You may provide a to-be-guided part and a guide part in a reverse container.

5-4. Plate-shaped body As shown in FIG. 11, the suction container 100 of the first container 6 has a first plate-shaped body 123 extending along the first flow path 2 a in the suction insertion portion 122.

  In addition, the first cleaning container 210 of the first container 6 has a second plate-like body 213 extending along the third flow path 2b in the first insertion portion 212.

  The first plate-like body 123 extends from the first flow path 2a into the third flow path 2b when the first flow path 2a and the third flow path 2b communicate with each other. The second plate-like body 213 extends from the third flow path 2b into the fourth flow path 2c when the first cleaning container 210 and the second cleaning container 220 are joined.

  The first plate-like body 123 and the second plate-like body 213 have the same shape. In order to avoid overlapping description, the first plate-like body 123 will be described.

  The first plate-like body 123 has a flow channel 2a for moving a target nucleic acid as a biological substance between the first inner surface 120a of the first flow channel 2a and the second inner surface 210a of the second flow channel 2b. Part of 2b is formed. The flat front surface and back surface of the first plate-like body 123 are located at a predetermined interval from the first inner surface 120a and the second inner surface 210a, and a part of the flow paths 2a and 2b is formed at the interval. Both end portions in the width direction of the first plate-like body 123 are formed integrally with the first inner surface 120a in the suction insertion portion 122 and in contact with the second inner surface 210a in the third flow path 2b. ing.

FIG. 15 is a DD cross-sectional view of the first container 6 in FIG. 11. As shown in FIG. 15, the first plate-like body 123 is arranged so that the two plates intersect. The two first plate-like bodies 123a and 123b have a cross-shaped cross section. Between the first plate-like body 123 and the first inner surface 120a (and similarly between the first plate-like body 123 and the second inner surface 210a), a first flow path 2a divided into four is formed. The Since the second plate-like body 213 is arranged at the same angle around the axis Xa as the first plate-like body 123, it does not appear in FIG.

  If each of the first plate 123 and the second plate 213 is one, the first plate 123 and the second plate with respect to the movement of the magnetic beads 30 in the horizontal direction (left and right in FIG. 11). The phase of the first cleaning container 210 should be set with respect to the adsorption container 100 and the phase of the second cleaning container 220 should be set with respect to the first cleaning container 210 so that the front surface or the back surface of the body 213 must face each other. I must. That is, the phase management between the adsorption container 100 and the first cleaning container 210 and the phase management between the first cleaning container 210 and the second cleaning container 220 at this time are both 180 degrees. On the other hand, the plurality of first plate-like bodies 123 and the second plate-like bodies 213, that is, two here, allows the phase management between the containers to be 90 degrees. When the degree of freedom in phase management is increased in this way, the joining operation is easy for the user, and the joining structure can be simplified for each container.

  Here, the angle around the axis Xa of each container in the first container 6 is set to a predetermined angle, and the first guide part 227a of the first joint part 227, the first plate-like bodies 123a and 123b, and the second plate. The angles around the axis Xa with the bodies 213a and 213b are in a desired positional relationship. The structure for setting the adsorption, first cleaning, and second cleaning containers 100, 210, and 220 in the first container 6 to a predetermined positional relationship will be described later.

  By arranging the first plate-like body 123 and the second plate-like body 213 in this way, when the first container 6 and the second container 7 are joined and set in, for example, a PCR device, the third flow path 2c to the third The magnetic beads 30 as the substance-binding solid phase carrier can be moved along the second plate-like body 213 to the flow path 2b, and the magnetic beads 30 are moved from the third flow path 2b to the first flow path 2a. It can move along the body 123. As described in “4-2. Magnet moving mechanism” above, even if the swinging mechanism only swings the pair of magnets 3 in the left-right direction in FIG. The first and second plate-like bodies 123 and 213 can be arranged. A desired position of the second container 7 is set around the axis Xb with respect to the PCR device by the phase management unit 237, and the first plate-like body 123 and the second of the first container 6 with respect to the second container 7. This is because the plate-like body 213 is set to a desired angle around the axis Xb.

  The moving method of the magnetic beads 30 is as shown in FIG. The magnetic beads 30 move along the first plate-like body 123 (the same applies to the second plate-like body 213) as the magnet 3 swings and moves upward as indicated by the arrow.

5-5. Phase Management Unit As shown in FIGS. 11 and 14, the third cleaning container 230 in the second container 7 includes a phase management unit 237.

  The phase management unit 237 is used to set a phase (position of the cartridge in the rotation direction about the axes Xa and Xb) to which the biological material purification cartridge is attached to the biological material purification apparatus such as a PCR device.

  The phase management unit 237 is a plate-like body that extends in a direction parallel to the axis Xb of the second flow path 2e and projects from the outer surface of the third cleaning container 230 (second container 7). An angle around the axis Xb of the second container 7 with respect to the biological material purification apparatus can be set by a plate-like body extending in a direction parallel to the axis Xb of the second flow path 2e.

FIG. 17 is a schematic diagram for explaining a state in which the phase management unit 237 is fixed to the biological material purification apparatus 51. The phase management unit 237 is an EE cross section of FIG. As shown in FIG. 17, the biological material purification apparatus 51 is provided with two grooves 52 for receiving two plate-like bodies of the phase management unit 237 at predetermined positions, and the width of the groove 52 is a plate-like body (237 ) Same or slightly wider than the thickness. And the 1st plate-shaped body 123 and the phase management part 237 which are shown with a broken line always become a fixed phase (angle around the axis | shaft Xb).

  The phase management unit 237 is provided at a position facing the axis Xb. Specifically, it is below the first guided portion 235a in the virtual plane including the axis Xb. Since the first guided part 235a and the phase management part 237 are in the same virtual plane, the axes Xa and Xb of the first container 6 and the phase management part 237 for guiding and joining the first guided part 235a The surrounding angles will match. In this way, the phase around the axis Xb of the second container 7 (biological material purification cartridge) when the user attaches the cartridge to the biological material purification apparatus can be managed at 180 degrees.

  Here, an example in which the first and second plate-like bodies 123 and 213 of the first container 6 and the phase management unit 237 of the second container 7 are set to a predetermined angle around the axis xb has been described. However, the present invention can be applied to other uses in which the first container 6 is disposed at a desired position with respect to the second container 7. For example, when a label for displaying the contents is attached to a predetermined position of the first container 6, the angle can be set around the axis Xb where the label is always visible when the cartridge is attached to the apparatus.

  In addition, the first container 6 is a state in which the adsorption container 100, the first cleaning container 210 and the second cleaning container 220 are joined, and the second container 7 is in a state in which the third cleaning container 230 and the elution container 300 are joined. However, the present invention is not limited to this, and each container may be separated or temporarily assembled. In that case, the fluid is sealed and stored for each flow path.

5-6. FIG. 18 is a schematic diagram for explaining the assembly structure of the first container 6, and shows a part of the second guide part 127 a, the third guide part 217 a, and the first guide part 227 a in a sectional view. ing.

  As shown in FIG. 18, the first container 6 is obtained by joining and assembling the adsorption container 100, the first cleaning container 210, and the third cleaning container 220. In each container, both ends of the flow path formed inside are sealed with a film (not shown), and the contents are stored in the flow path.

  The suction cover portion 126 at the lower end of the suction container 100 has a second guide portion 127a and a second vertical groove 127c formed on the inner surface 126a, and a second hole 127b penetrates from the inner surface 126a to the outer surface.

  Second guided portions 215a and 215b protrude from the middle outer surface of the first cleaning container 210. The first cover portion 216 at the lower end of the first cleaning container 210 has a third guide portion 217a and a third vertical groove 217c formed on the inner surface 216a, and a third hole 217b penetrates from the inner surface 216a to the outer surface.

  Third guided portions 225 a and 225 b protrude from the outer surface of the second stage of the second cleaning container 220. The second cover part 226 at the lower end of the second cleaning container 220 has a first guide part 227a and a first vertical groove 227c formed on the inner surface 226a, and the first hole 227b penetrates from the inner surface 226a to the outer surface.

  The second guide part 127a, the second hole 127b, and the second vertical groove 127c, and the third guide part 217a, the third hole 217b, and the third vertical groove 217c are the first guide part 227a, the first hole 227b, and the first hole, respectively. It is the same shape as 1 vertical groove 227c.

The second guided portions 215a and 215b and the third guided portions 225a and 225b have the same shape as the first guided portions 235a and 235b, respectively.

  Accordingly, the second guide part 127a rotates in contact with the second guided parts 215a and 215b when the adsorption container 100 and the first cleaning container 210 are joined to each other. The angle around the axis Xa of the third flow path 2b (see FIG. 11) can be set.

  In addition, when the first cleaning container 210 and the second cleaning container 220 are joined, the third guide part 217a rotates in contact with the third guided parts 225a and 225b and the second guide part 217a with respect to the first cleaning container 210. The angle around the axis Xa of the fourth flow path 2c (see FIG. 11) of the cleaning container 220 can be set.

  Here, an example in which the first cleaning container 210 and the second cleaning container 220 are provided to the user in a separated state and the user assembles them has been described. However, the present invention is not limited thereto, and the first cleaning container 210 and the second cleaning container It may be a cleaning container in which the container 220 is joined in advance.

  Thus, according to the biological material purification cartridge set 5a, the angle around the axis Xb between the second cleaning container 220 and the second container 7 can be set, and the second cleaning container 220, the adsorption container 100, and the first An angle around the axis Xa with the cleaning container 210 can be set. Therefore, the angles around the axes Xa and Xb of the adsorption container 100, the first cleaning container 210, the second cleaning container 220, and the second container 7 can be set. In other words, the first plate-like body 123 and the second plate-like body 213 can be set at a predetermined angle around the axes Xa and Xb with respect to the PCR device, for example. Then, due to the relationship between the first container 6 and the second container 7, the first and second plate-like bodies 123 and 213 are set in a direction that matches the swinging direction in the magnet moving mechanism of the PCR device.

  Further, when the magnetic beads 30 shown in FIG. 11 and the like are stored for a long time together with the chaotropic substance, the function of adsorbing the target nucleic acid is lowered. Usually, the adsorbing liquid 10 contains a chaotropic substance, and even if the adsorbing liquid 10 is held in a plug shape by the first oil 20, the movement of the chaotropic substance at the molecular level cannot be completely prevented. Therefore, it is desirable to store the magnetic beads 30 in a container different from the adsorption container 100 in which the adsorption solution 10 is sealed and stored until immediately before the nucleic acid extraction step. Since the magnetic beads 30 are fine particles as described above, introduction into the adsorption container 100 manually reduces the work efficiency. Therefore, the workability can be improved by using the first container 6 previously held in the cleaning container 200a. Further, the cleaning liquid may contain a slight amount of chaotropic substance. In that case, a second cleaning container 220 different from the first cleaning container 210 containing the first cleaning liquid 12 containing the chaotropic substance as in the first container 6 is prepared, and the magnetic beads 30 are placed in the second cleaning container 220. It is desirable to keep it inside. The second cleaning liquid 14 in the second cleaning container 220 does not contain a chaotropic substance. In this case, the first and second plate-like bodies 123 and 213 are required for the suction insertion portion 122 of the suction container 100 and the first insertion portion 212 of the first cleaning container 210.

5-7. FIG. 19 is a schematic diagram for explaining the assembly structure of the second container 7.

  As shown in FIG. 19, the first guided portion 235 a is provided at one end of the third cleaning container 230. The third cleaning container 230 has a fourth guide portion 238a at the other end.

The elution container 300 has a fourth guided portion 305 guided by the fourth guide portion 238a. When the third cleaning container 230 and the elution container 300 are joined, the fourth guide part 238a contacts the fourth guided part 305 and the axis of the second flow path 2e of the elution container 300 with respect to the third cleaning container 230. Set the angle around Xb. In this way, the angle around the axis Xb between the elution container 300 and the third cleaning container 230 can be set, and the axis Xa between the third cleaning container 230 and the first container 6 can be set.
And an angle around the axis Xb can be set. Therefore, the angles around the axis Xa and the axis Xb between the first container 6, the third cleaning container 230, and the elution container 300 can be set.

  The fourth guided portion 305 is a fourth groove 305 b provided in a fourth flange 305 a that protrudes from the outer surface of the elution container 300 in a direction orthogonal to the axis Xb.

  The fourth guide portion 238a is a plate-like body that extends in the direction of the axis Xb of the third cleaning container 230, and is formed so as to increase in width from the distal end 238d toward the proximal end 238c. The width of the base end 238 c of the fourth guide portion 238 a is the same as the width of the fourth groove 305 b of the fourth guided portion 305.

  When the elution container 300 and the third cleaning container 230 are joined, if the tip 238d of the fourth guide part 238a is inserted into the fourth groove 305b, the fourth guide part 238a is centered on the axis Xb in the fourth groove 305b. The angle around the axis Xb can be set by proceeding along the axis Xb while being guided in the rotating direction, and the base end 238c entering the fourth groove 305b. The fourth protrusion 305c provided in the vicinity of the center of the fourth groove 305b enters the fourth hole 238b provided in the base end 238c, so that the third cleaning container 230 and the elution container 300 along the axis Xb. Both the movement in the selected direction and the movement in the direction of rotation about the axis Xb are restricted, and both containers are fixed. The phase management around the axis Xb between the fourth guide portion 238a and the fourth guided portion 305 is 180 degrees. You may employ | adopt this structure for the 1st junction part and the 2nd junction part 235. FIG. Further, the fourth groove 305b is not limited to the groove, and may be a through hole having a predetermined width.

6). Modified Example The phase management unit 237 in the second container 7 of the above embodiment sets the phase (position of the cartridge in the rotation direction about the axes Xa and Xb) for attaching the biological material purification cartridge to the PCR device or the like. Is for. Therefore, the structure of the phase management unit 237 often depends on the structure of the apparatus side to which the cartridge is attached.

  FIG. 20 is a perspective view for explaining a modified example of the third cleaning container 230.

  As shown in FIG. 20, the phase management unit 237a is a groove extending in a direction parallel to the axis Xb. The second container 7 can be set at a predetermined angle around the axis Xb with respect to the biological material purifying apparatus by a simple structure called a groove (237a) extending in a direction parallel to the axis Xb. In this case, the biological substance refining device is provided with a projection that fits into the groove (237a) at a predetermined angle. The phase management unit 237a is provided at a predetermined position of the third cleaning container 230.

  FIG. 21 is a perspective view for explaining another modified example of the third cleaning container 230.

  As shown in FIG. 21, the phase management unit 237 b is a mark provided on the outer surface of the second container 7. The mark may be printed in advance so as to be visible with ink, or may be a small groove or protrusion. Such a mark provided at a predetermined position on the outer surface of the second container 7 allows the user to set the second container 7 at a predetermined angle around the axis Xb with respect to the biological material purification apparatus. In the biological substance purification apparatus, a mark or the like for alignment with this mark is provided at a predetermined position.

The present invention is not limited to the above-described embodiments, and various modifications can be made. For example, the present invention includes configurations that are substantially the same as the configurations described in the embodiments (for example, configurations that have the same functions, methods, and results, or configurations that have the same objects and effects). In addition, the invention includes a configuration in which a non-essential part of the configuration described in the embodiment is replaced. In addition, the present invention includes a configuration that exhibits the same operational effects as the configuration described in the embodiment or a configuration that can achieve the same object. In addition, the invention includes a configuration in which a known technique is added to the configuration described in the embodiment.

DESCRIPTION OF SYMBOLS 1 ... Container assembly, 2 ... Flow path, 2a ... 1st flow path, 2b ... 3rd flow path, 2c ... 4th flow path, 2d ... 5th flow path, 2e ... 2nd flow path, 3 ... Magnet, DESCRIPTION OF SYMBOLS 5 ... Biological-related substance refinement | purification cartridge set, 6 ... 1st container, 7 ... 2nd container, 10 ... Adsorption liquid, 11 ... Air, 12 ... 1st washing | cleaning liquid, 14 ... 2nd washing | cleaning liquid, 16 ... 3rd washing liquid, 20 ... 1st oil, 22 ... 2nd oil, 24 ... 3rd oil, 26 ... 4th oil, 30 ... Magnetic beads, 32 ... Eluate, 34 ... Reagent, 36 ... Droplet, 50 ... PCR apparatus, 55 ... Fluorescence measurement Apparatus 60 ... Rotation mechanism, 65 ... Heater, 66 ... Rotation motor, 70 ... Magnet movement mechanism, 80 ... Pressing mechanism, 90 ... Controller, 100 ... Adsorption container, 110 ... Cap, 112 ... Ventilation part, 120 ... Syringe part, 120a ... 1st inner surface, 120b ... Flange part, 120c ... Fi 122, suction insertion portion, 122a, outer wall, 122c, film, 123, first plate, 123a, first plate, 123b, first plate, 126, suction cover, 126a, inner surface, 127a ... second guide portion, 127b ... second hole, 127c ... second longitudinal groove, 130 ... plunger portion, 132 ... bar-shaped portion, 134 ... tip portion, 200 ... cleaning container, 200a ... cleaning container, 210 ... first Cleaning container, 210a ... second inner surface, 212 ... first insertion portion, 212a ... outer wall, 213 ... second plate-like body, 213a ... second plate-like body, 213b ... second plate-like body, 214 ... first receiving portion 215a ... second guided portion, 215b ... second guided portion, 216 ... first cover portion, 216a ... inner surface, 217a ... third guide portion, 217b ... third hole, 217c ... third longitudinal groove, 220 ... Second cleaning container, 222 ... second insertion part 222c ... film, 224 ... second receiving part, 225a ... third guided part, 225b ... third guided part, 226 ... second cover part, 226a ... inner surface, 226b ... step part, 227 ... first joint part, 227a ... 1st guide part, 227b ... 1st hole, 227c ... 1st vertical groove, 230 ... 3rd washing container, 230c ... film, 232 ... 3rd insertion part, 234 ... 3rd receiving part, 235 ... 2nd joining Part, 235a ... first guided part, 235b ... first guided part, 236 ... third cover part, 237 ... phase management part, 238a ... fourth guide part, 238b ... fourth hole, 238c ... proximal end, 238d ... tip 300 ... elution container 302 ... elution insertion part 304 ... elution receiving part 305 ... 4th guided part 305a ... 4th flange, 305b ... 4th groove, 305c ... 4th protrusion, 306 ... elution cover Part, 306c ... Phil 400 ... reaction vessel 402 ... bottom part 404 ... reaction receiving part 405 ... reaction cover part 406 ... reservoir part 510 ... first temporary assembly Xa ... axis Xb ... axis

Claims (14)

A biological substance purification cartridge set to be attached to a biological substance purification apparatus,
A first container having a first flow path in which a first fluid is sealed and stored;
A second container having a second flow path in which a second fluid is sealed and accommodated, connected to the first container and communicating with the first flow path;
Including
Either the first fluid or the second fluid is a fluid for purifying a substance,
The first container has a first joint part in which a guide part is formed,
The second container includes a second joint portion in which a guided portion guided by the guide portion is formed, and a phase management portion that sets an angle around the axis of the second flow path with respect to the biological material purification apparatus. Have
The guide section is configured to set an angle around the axis of the first container with respect to the second container by contacting the guided section when the first container and the second container are joined. Substance purification cartridge set. In claim 1,
The guided portion is formed at one or more locations in the second joint portion,
The first joint part is a biological material purification cartridge set in which the guide part is formed every 90 degrees at four locations around the axis of the first flow path. In claim 1 or 2,
The guided portion is a protrusion protruding from the outer surface of the second joint portion,
The guide part is a groove formed on the inner surface of the first joint part,
The biological material purification cartridge set, wherein the protrusion is guided in contact with a side surface of the groove. In any one of Claims 1 thru | or 3,
The first container includes an adsorption container that forms the first flow path in which an adsorbing liquid is sealed and stored,
The second container includes an elution container that forms the second flow path in which an eluate is sealed and stored,
The adsorbent is a liquid that adsorbs a biological substance on a substance-binding solid phase carrier,
The biologically relevant substance purification cartridge set, wherein the eluent is a liquid that elutes biologically relevant substances from the substance-binding solid phase carrier into the eluate. In claim 4,
The first container further includes a cleaning container that has a third channel that communicates with the first channel and contains a cleaning liquid for cleaning the substance-binding solid phase carrier on which the biological substance is adsorbed. set. In claim 5,
The adsorption container has a plate-like body extending along the first flow path,
The plate-like body extends from the first flow path into the third flow path when the first flow path and the third flow path communicate with each other, and the first flow path from the third flow path to the first flow path. A biological material purification cartridge set that enables a substance-binding solid phase carrier to move along a plate-like body to a flow path. In claim 5 or 6,
The guide part is a first guide part provided at one end of the cleaning container,
The adsorption container has a second guide part,
The cleaning container has a second guided portion guided to the second guiding portion at the other end,
The second guide part is in contact with the second guided part when the adsorption container and the cleaning container are joined to each other, and the relative angle around the axis of the third flow path of the cleaning container with respect to the adsorption container Set the bio-related substance purification cartridge set. In claim 6 or 7,
The plate-like body is a first plate-like body,
The cleaning container includes a first cleaning container having the third flow path and a second cleaning container having a fourth flow path,
The first cleaning container has a second plate-like body extending along the third flow path,
The second plate-like body extends from the third flow path into the fourth flow path when the first cleaning container and the second cleaning container are joined, and from the fourth flow path, A biological material purification cartridge set that enables a substance-binding solid phase carrier to move along the second plate-like body to a third channel. In claim 8,
The guide part is a first guide part provided at one end of the second cleaning container,
The first cleaning container has a third guide part,
The second cleaning container has a third guided portion guided by the third guide portion at the other end,
The third guide part contacts the third guided part when joining the first cleaning container and the second cleaning container, and the fourth guide part of the second cleaning container with respect to the first cleaning container is in contact with the fourth cleaning part. A biological material purification cartridge set that sets the angle around the axis of the flow path. In any one of Claims 4 thru | or 9,
The second container further includes a third cleaning container having a fifth flow path communicating with the second flow path,
The guided portion is a first guided portion, provided at one end of the third cleaning container,
The third cleaning container has a fourth guide at the other end,
The elution container has a fourth guided portion guided by the fourth guide portion,
The fourth guide part contacts the fourth guided part when the third cleaning container and the elution container are joined, and the shaft of the second flow path of the elution container with respect to the third cleaning container A bio-related substance purification cartridge set that sets the surrounding angle. In claim 10,
The fourth guided portion is a groove provided on the outer surface of the elution container,
The fourth guide portion is a plate-like body extending in the axial direction of the third cleaning container, and is formed so that the width increases from the distal end toward the proximal end,
The width of the base end of the fourth guide part is the same as the width of the groove of the fourth guided part. In any one of Claims 1 thru | or 11,
The biorelevant substance purification cartridge set, wherein the phase management unit is a plate-like body that extends in a direction parallel to the axis of the second flow path and protrudes from the outer surface of the second container. In any one of Claims 1 thru | or 11,
The phase management unit is a biological material purification cartridge set that is a groove extending in a direction parallel to the axis of the second flow path. In any one of Claims 1 thru | or 11,
The phase management unit is a biological material purification cartridge set, which is a mark provided on the outer surface of the second container.