JP2016121119A - Amino acid separation method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an amino acid separation method capable of stably separating amino acid as an element in a state close to an in vivo environment without use of chemical reaction.SOLUTION: An amino acid separation method includes the steps of: preparing a water solution by melting plural kinds of amino acid mixtures A, A, and Ainto water, regarding the water solution as an acid solution (or alkali solution), and electrifying the amino acids A, A, and Awith positive (or negative) charge; generating a stream v in the acid solution 10 (or alkali solution) coaxially and concentrically so as to induce a magnetic field B in the acid solution (or alkali solution), coaxially and concentrically collecting the amino acids A, A, and Ain the respective radii r, r, and r, in which a centrifugal force derived from the stream v and a Lorentz's force derived from the magnetic field B are balanced, so as to form and distribute plural cylindrical amino acid element layers AS, AS, and AS; and selectively or concurrently separately taking out the plural cylindrical amino acid element layers AS, AS, and AS.SELECTED DRAWING: Figure 2

Description

  The present invention relates to a method for separating amino acids. Specifically, at least a mixture of at least a plurality of amino acids is dissolved in water to form an aqueous solution, the aqueous solution is converted to an acid solution or an alkaline solution, and centrifugal force and Lorentz force It is related with the separation method of the amino acid which isolate | separates into each kind of the amino acid using the balance of, and takes out as a simple substance of an amino acid.

In general, amino acid separation methods use various chemical reactions, which requires a process of adding a large amount of various chemical substances and then removing those substances and by-products derived from those substances. It was.
The general formula of the molecule of this amino acid is R—CH (NH ₂ ) COOH, and the type of amino acid varies depending on the difference in R. For example, if R is H, that is, hydrogen, it becomes H—CH (NH ₂ ) COOH, which is called glycine, and if R is CH ₃ , it becomes CH ₃ —CH (NH ₂ ) COOH, and alanine and be called.
The three-dimensional structure of this amino acid has a triangular pyramid structure. And what this amino acid peptide-bonded is protein.
This protein is hydrolyzed with a dilute acid, an alkaline solution, an enzyme (trypsin) or the like, so that its peptide bond is broken and it is decomposed into individual amino acids as a simple substance.
Conventional amino acid separation methods generally utilize such chemical reactions.

  (Non) Patent Literature n

  The object of the present invention is to be able to separate amino acids in a state close to the environment in the living body without using a chemical reaction that adds a large amount of chemical substances, and to simplify and simplify the separation process and apparatus. In addition to being able to be separated stably and effectively and efficiently, separation time can be shortened and cost can be reduced. Is in the provision of.

  In the method for separating amino acids of the present invention, an aqueous solution in which a mixture of a plurality of types of amino acids is dissolved in water is prepared in advance, and the aqueous solution is made into an acid solution or an alkaline solution and placed in a vertical cylindrical water tank, or Putting the aqueous solution into the water tank and converting the aqueous solution into an acid solution or an alkaline solution in the water tank, and then rotating the water tank around the longitudinal axis of the water tank to turn into the acid solution or the alkaline solution. Create a water flow and apply a magnetic field to the acid or alkaline solution from either the top or bottom of the tank in the direction of the longitudinal axis, and the radial direction perpendicular to the longitudinal axis Concentrically gather amino acids as single amino acids at each amino acid that balances centrifugal force due to water flow and Lorentz force due to its magnetic field. Forming a cylindrical individual amino acids layer number, and includes a selectively retrieve or simultaneously separately retrieve process the plurality of cylindrical amino alone layer.

  In addition, the amino acid separation method of the present invention that can be embodied in a simpler and simpler process and apparatus is an aqueous solution in which a mixed solution of a plurality of amino acids is dissolved in water in advance, and carbon dioxide is further added to the aqueous solution. Bubbling and making the aqueous solution into an acid solution into a vertical cylindrical water tank, then rotating the water tank about the longitudinal axis of the water tank to create a water flow in the acid solution; and Applying a magnetic field to the acid solution from either the top or bottom direction of the water tank in the direction of the vertical axis, simultaneously with the rotation of the water tank or before or after the rotation of the water tank, and the vertical axis As a single amino acid, concentrically collect amino acids at the balance between the centrifugal force caused by the water flow and the Lorentz force caused by the magnetic field. Forming a cylindrical individual amino acids layer number, and includes a selectively retrieve or simultaneously separately retrieve process the plurality of cylindrical amino alone layer.

  The effect of the present invention is that by using a balance between centrifugal force and Lorentz force without using a chemical reaction that adds a large amount of chemical substances, first of all, as in the case of using such a chemical reaction. It is not necessary to add a large amount of chemical substances or a large amount of chemical substances and then remove them and by-products derived from them. Multiple amino acids are separated into amino acids in a state close to the environment in the living body. In addition to making the process and equipment simpler and easier to materialize and allowing the simple and simplified process and equipment to stably separate amino acids as a single unit, As a result, the separation time can be shortened, the cost is reduced, and the amino acid is present as a single substance after the separation. Use in the place where it becomes possible remains.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a perspective view for explaining a basic concept of an amino acid separation method according to the present invention. 1 is a perspective view partially showing a specific example of an amino acid separation method and separation apparatus according to the present invention. FIG. FIG. 3 is a side view showing a partial specific example shown in FIG. 2. BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic diagram showing an overall example of an amino acid separation method and separation apparatus according to the present invention. BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic view showing an overall example of an amino acid separation method and separation device of the present invention in which an amino acid extraction device uses a force generated from a difference in concentration. FIG. 5 is a perspective view partially showing another specific example of the amino acid separation method and separation apparatus of the present invention. FIG. 7 is a side view showing another specific example shown in FIG. 6. FIG. 7 is a schematic diagram showing the other specific example shown in FIG. 6 as a whole.

  The method for separating amino acids of the present invention is based on a technique that utilizes a balance between centrifugal force and Lorentz force, and an embodiment of the method is an aqueous solution in which a mixture of a plurality of types of amino acids is dissolved in water in advance, and Furthermore, the aqueous solution is made into an acid solution or an alkaline solution and placed in a bowl-shaped cylindrical water tank, or the aqueous solution is placed in the water tank and the aqueous solution is converted into an acid solution or an alkaline solution in the water tank. In addition, the water tank is rotated around the vertical axis of the water tank to generate a water flow in the acid solution or the alkaline solution, and in the direction of the vertical axis, from either the top or bottom direction of the water tank or the vertical direction A magnetic field is applied to the acid solution or alkaline solution, and the centrifugal force due to the water flow and the Lauren due to the magnetic field in the radial direction perpendicular to the longitudinal axis. A process of concentrically collecting amino acids as single amino acids at each of the balanced amino acids to form a plurality of cylindrical amino acid single layers, and selectively removing the multiple cylindrical amino acid single layers or simultaneously separating them It is embodied in the place including the step of taking out.

  In addition, the amino acid separation method of the present invention is an aqueous solution in which a mixed solution of a plurality of types of amino acids is dissolved in water in advance, and further, carbon dioxide is bubbled into the aqueous solution, and the aqueous solution is converted into an acid solution to form a saddle type. The step of placing in a cylindrical aquarium, then rotating the aquarium about the longitudinal axis of the aquarium to produce a flow of water in the acid solution and either simultaneously with the aquarium rotation or before the aquarium rotation Alternatively, after rotation, a magnetic field is applied to the acid solution from either the top or bottom direction of the water tank in the direction of the vertical axis, and the centrifugal force due to the water flow and the magnetic field in the radial direction perpendicular to the vertical axis. A process of concentrically collecting amino acids as single amino acids at each of the amino acids that balance Lorentz's force, and forming a plurality of cylindrical amino acid single layers, and It includes the plurality of cylindrical amino single layer selectively retrieve or simultaneously separately retrieve process is embodied in simpler and more simple process.

Hereinafter, the method for separating amino acids according to the present invention will be described based on the specific examples specified and illustrated. FIGS. 1 to 4 show the amino acids for separating amino acids as a simple substance from a mixture of many kinds of amino acids. 10 shows a specific example 10 of the separation method and separation apparatus.
First, with reference to FIG. 1, the basic idea that the method for separating amino acids of the present invention uses the balance between centrifugal force and Lorentz force will be described.
Amino acids, although the molecular formula is _{R-CH (NH 2) COOH} , in the solution of acid, positively charged becomes _-NH ^{3 +,} also in the alkaline solution, -COOH ^- negatively becomes When the solution is made either acidic or alkaline to be charged, it exists as a charged ion, ie a cation or an anion.
As shown in FIG. 1, an amino acid is previously dissolved in water to form an aqueous solution, and the aqueous solution is further made into an acid solution. The amino acid that dissolves in this acid solution is positively charged. Then, the acid solution is put into the vertical cylindrical water tank 11.
Then, as shown in FIG. 1, the vertical cylindrical water tank 11 is rotated clockwise around its longitudinal axis 0 at a predetermined rotational speed, and a water flow v is clockwise applied to the acid solution. Then, in that state, a magnetic field B is generated from the bottom of the water tank 11 upward in the direction of the vertical axis 0 of the water tank 11, and the magnetic field B is applied to the acid solution.
Here, the charge of the amino acid is q (> 0), the mass is m, the magnetic field strength B, and the velocity of the water flow (velocity of amino acid molecule) is v (m / s). However, the velocity v of the water flow (water as a solvent) is slow (small) at the center of the water tank 11 and fast (large) at the inner side surface of the water tank 11.
Under such a condition, as shown in FIG. 1, the amino acid present in the radial direction R perpendicular to the vertical axis 0 of the aquarium 11, that is, at the position of the radius r from the center of the aquarium 11, The centrifugal force m · v ² / r (N) due to the water flow v and the Lorentz force q · v · B (N) due to the magnetic field B work in opposite directions, and the position where both are just balanced is r and this position is Amino acids are collected in the cylindrical amino acid single layer AS in the water tank 11 and formed and distributed.
This can be expressed by the equation of motion.
m · v ² / r = q · v · B (N)
Is the position (distance) where the centrifugal force and the Lorentz force are balanced, and the radius r of the distance from the center 0 of the aquarium 11 is
r = (m / q) · (v / B) (m)
Therefore, if v and B are constant, the radius r is determined by the mass m and charge q of the amino acid. That is, since amino acids have different masses m and charges q depending on the types, the positions r at which the amino acids gather in a distribution form are unique to each type of amino acid.
Utilizing this property for amino acid separation is the basic concept of the method for separating amino acids of the present invention.
Therefore, a specific example 10 of the amino acid separation method and separation apparatus of the present invention, which is embodied in a simple and simple process and apparatus, will be described.
This amino acid separation method 10 is a step of dissolving a mixed solution of various kinds of amino acids A ₁ , A ₂ , A ₃ in water, making it into an acid solution and putting it in a bowl-shaped cylindrical water tank 15, A magnetic field B is applied to the charged acid solution, that is, the various amino acids A ₁ , A ₂ , A ₃ that are dissolved in the acid solution and charged positively from the bottom of the water tank 15 upward, On the other hand, the water tank 15 is rotated clockwise about its longitudinal axis 0, and a water flow v is generated in the acid solution in the water tank 15 in the clockwise direction, and the various amino acids A ₁ , A ₂ and A ₃ are separated into a single cylindrical layer AS ₁ , AS ₂ and AS ₃ in a concentric manner and collected, and the multiple types of cylindrical single layers AS ₁ , AS ₂ and AS ₃ are simultaneously separated. The process to take out in order, and The amino acid separator 10 includes a vertical cylindrical water tank 15 that is rotated clockwise by an electric motor (not shown) using a carbon dioxide bubbling device (not shown) and a speed reducer (not shown), and a magnetic field. The generator 17 and the amino acid take-out devices 19, 20, 21 are assembled.

In the separation method 10 of this amino acid, the step of placing a solution of the acid into its water tank 15 form the solution of the acid containing the various kinds of amino acids A _1, A _2, A ₃ in advance that many kinds of amino acids A ₁ , A ₂ , A _{3 in} an aqueous solution in water, and bubbling carbon dioxide into the aqueous solution to make the aqueous solution into the acid solution, and then the acid solution into the water bath 15. Put in. Of course, carbon dioxide is produced by the carbon dioxide bubbling device.

In the process of separating and collecting each of the various types of amino acids A ₁ , A ₂ , A ₃ into simple substances, the various types of amino acids A ₁ , A ₂ , A ₃ are positively charged in the water tank 11 and the acid The magnetic field generator 17 applies the magnetic field B to the cation amino acid molecules A ₁ , A ₂ , A ₃ from the bottom of the water tank 15 in the upward direction through the speed reducer. The electric motor causes the water tank 15 to rotate clockwise around the longitudinal axis 0 at a predetermined speed, causing the water solution to flow clockwise in the acid solution in the water tank 15, and the vertical direction of the water tank 15. In the radial direction R perpendicular to the axis 0, the radii r _{1 of} the amino acids A ₁ , A ₂ , A _3, respectively, in which the centrifugal force due to the water flow v and the Lorentz force due to the magnetic field B are balanced as amino acids alone, The various types of amino acids A ₁ , A ₂ , A ₃ are separated and collected at r ₂ , r ₃ , and formed into a plurality of types of cylindrical amino acid monolayers AS ₁ , AS ₂ , AS _3. Distribute. Of course, in this process, even if the work of applying the magnetic field B and the work of rotating the water tank 15 are performed simultaneously, one of them is performed first, and the other is performed later. It doesn't matter.

Thus, in this step, the charge of the amino acid molecule is q (> 0) [C] and the mass is m, and the magnetic field B with the magnetic flux density B (W / m ² ) is applied to the water tank as shown in FIGS. When the water tank 15 is rotated from the bottom to the top and rotated in the clockwise direction about the longitudinal axis 0, the frictional force generated between the inner wall surface of the water tank 15 and the acid solution causes the acid to flow. The solution begins to rotate clockwise.
What is important here is that in the radial direction R perpendicular to the longitudinal axis 0 of the water tank 15, the water as the amino acid and the solvent located at a radius (distance) r from the center 0 of the water tank 15 rotates the water tank 15. By having the same rotational angular velocity as the angular velocity, for example, a difference occurs in the rotational angular velocity of the amino acid and the solvent water, and if the rotational angular velocity of the solvent water becomes excessively high, the solvent water The density of the water tank 15 is low at the center of the water tank 15 and high at the side wall of the water tank 15, so that the central part is under negative pressure, and amino acids that do not have sufficient speed cannot obtain centrifugal force. As a result, it is pulled into the center.
More importantly, the water as the solvent rotates in the water tank 15 in the radial direction R perpendicular to the vertical axis 0 from the center to the side wall at the same angular velocity, so that the amino acid flows in addition to the flow. In other words, the state of moving in the rotational direction from the central portion to the side wall portion is maintained without being affected by other simultaneous flows.
For this purpose, the water tank 15 has a circular center pole 16 having a circular cross section at the center of the water tank 15 arranged coaxially with the longitudinal axis 0 thereof.

The step of separately taking out the multiple types of cylindrical amino acid single layers AS ₁ , AS ₂ , AS ₃ is performed by using the plurality of amino acid extraction devices 19, 20, 21 with the multiple types of amino acid single layers AS ₁ , AS ₂ , AS. ₃ Aspirate each separately. Each of the amino acid _{A 1,} A 2, _{A 3} through those steps are taken separated from a mixture of amino acids _{A 1,} A 2, _{A 3} as a unit.

The amino acid separation device 10 for performing this amino acid separation method 10 is shown in FIGS. 2 to 4 and, as described above, a carbon dioxide bubbling device (not shown), a saddle type cylinder The water tank 15, the magnetic field generator 17, and the amino acid extraction devices 19, 20, 21 are assembled.
The bowl-shaped cylindrical water tank 15 is made into a large bowl-shaped cylindrical container that is supported rotatably about the longitudinal axis 0 with the upper end open at the bottom wall 12 and the cylindrical side wall 13, and It is rotated clockwise by an electric motor (not shown) through a speed reducer (not shown).

  Further, the water tank 15 is integrally provided with a center pole 16 having a circular cross section. The center pole 16 is disposed coaxially with the longitudinal axis 0 of the water tank 15 in the water tank 15, extends from the bottom wall 12 to the opening 14, and is fixed to the bottom wall 12. . The water tank 15 does not disturb the water flow v so that the difference in the magnitude of the frictional force between the inner side surface of the cylindrical side wall 13 and the center of the water tank 15 can be ignored by the circular center pole 16 of the cross section. I am doing so.

  The magnetic field generator 17 includes an electromagnetic coil 18, a control panel (not shown), a power source (not shown), and the like. The electromagnetic coil 18 is arranged on the same axis directly below the water tank 15. Connected to the power supply at the control panel. 2, the water tank 15 is rotated clockwise as shown in FIG. 2, and the water flow v is generated clockwise. Therefore, when an electric current is applied by operating the control panel, the electromagnetic coil 18 A magnetic field B is generated from the bottom to the top. Of course, the electromagnetic coil 18 may be disposed both above and below the water tank 15 and either above or below.

As shown in FIG. 4, the amino acid take-out devices 19, 20, and 21 are amino acid containers 22, 23 and 24, pumps 25, 26 and 27, electric motors 28, 29 and 30, suction pipes 31, 32 and 33, and discharge pipes. 34, 35, 36, a control panel (not shown), a power source (not shown), etc., and the electric motors 28, 29, 30 are directly connected to the pumps 25, 26, 27 correspondingly. Connected to the power supply at the control panel.
The electric motors 28, 29, 30 are driven by the operation of the control panel, the electric motors 28, 29, 30 are driven, and the pumps 25, 26, 27 are driven by the electric motors 28, 29, 30. It is operated at 29,30. The pumps 25, 26, 27 are separated into the water tank 15, and the amino acids A ₁ , A ₂ , A ₃ collected in the cylindrical amino acid simple substance layers AS ₁ , AS ₂ , AS ₃ are collected in the amino acid containers 22, 23. , 24 and sucking in correspondingly.

Next, the operation of the amino acid separation apparatus 10 will be described.
In this amino acid separation apparatus 10, first, a mixed solution of the various types of amino acids A ₁ , A ₂ , A ₃ is dissolved in water to form an aqueous solution, and carbon dioxide (CO ₂ ) is bubbled into the aqueous solution. The aqueous solution is made into an acid solution.
Thus, when the aqueous solution is made into an acid solution, the amino acids A ₁ , A ₂ , A ₃ are dissolved in the acid solution in a positively charged state, that is, in a cation state. . In this state, the acid solution is put into the water tank 15. Here, the bubbling of the carbon dioxide (CO ₂ ) is performed by the carbon dioxide bubbling apparatus (not shown). The carbon dioxide bubbling device is assembled with a number of small-bore bubbling tubes (not shown), a carbon dioxide supply source (not shown), a pump (not shown), a connection pipe (not shown), and the like.

Next, the magnetic field generator 17 is operated on its control panel, and the electromagnetic coil 18 is energized. The electromagnetic coil 18 generates a magnetic field B from the bottom of the water tank 15 upward, and the water tank 15. The magnetic field B is applied to the amino acids A ₁ , A ₂ , and A ₃ that are dissolved in the acid solution and are positively charged and exist in the cation state.
At the same time or subsequently, the water tank 15 is operated by its control panel, and the electric motor is energized to drive the electric motor. The water tank 15 is rotated clockwise around the longitudinal axis 0 by the electric motor via the speed reducer, and a water flow v is generated clockwise in the acid solution in the water tank 15.
As such, a magnetic field B is applied to the acid solution in the aquarium 15 and at the same time a water flow v is generated, so that the amino acids A ₁ , A ₂ , A ₃ are on the vertical axis 0 of the aquarium 15. In the radial direction R orthogonal to each other, the amino acid A ₁ is located at the radii r ₁ , r ₂ , r ₃ of the amino acids A ₁ , A ₂ , A _3, respectively, in which the centrifugal force due to the water flow v and the Lorentz force due to the magnetic field B are balanced. , A ₂ and A ₃ are separated and collected, and are formed and distributed in the cylindrical amino acid single layer AS ₁ , AS ₂ and AS ₃ .

Each of the amino acids A ₁ , A ₂ , A ₃ is separated and collected in the water tank 15 at its radii r ₁ , r ₂ , r ₃ , and the cylindrical amino acid monolayer AS ₁ , AS ₂ , Since it is formed and distributed in AS ₃ , the cylindrical amino acid single layer AS ₁ , AS ₂ , AS ₃ is taken out separately by the amino acid take-out devices 19, 20, 21.
The amino acid take-out devices 19, 20, and 21 first adjust the suction pipes 31, 32, and 33 correspondingly to the single amino acid layers AS ₁ , AS ₂ , and AS ₃ , and then operate the control panel thereof. The electric motors 28, 29 and 30 are energized.
Since the electric motors 28, 29, 30 are driven, the pumps 25, 26, 27 are respectively operated by the corresponding electric motors 28, 29, 30, and are separated into the water tank 15. The cylindrical amino acid simple substance layers AS ₁ , AS ₂ , AS ₃ formed and separated from the amino acids A ₁ , A ₂ , A ₃ are sucked correspondingly, and the sucked amino acids A ₁ , A ₂ are sucked correspondingly. , correspondingly add lead to _{a 3} in the amino acid container 22, 23, 24.
As described above, the amino acid separation device 10 is utilized, and the amino acids A ₁ , A ₂ , and A ₃ are separated into amino acids from the mixed solution in a state close to the environment in the living body.

In this amino acid separation method and separation apparatus 10, carbon dioxide (CO ₂ ) is bubbled in an aqueous solution obtained by dissolving a mixture of the various types of amino acids A ₁ , A ₂ , and A ₃ in water before being transferred to the water tank 15. However, the carbon dioxide may be bubbled in the water tank 15 to form an acid solution after the aqueous solution is put in the water tank 15. In that case, it is desirable that the bubbling of the carbon dioxide is performed so as not to affect the water flow v. Further, this aqueous solution may be appropriately made into an acid solution so that the separation can be performed stably in a state close to the environment in the living body without adding a large amount of various kinds of chemical substances. On the other hand, when the alkaline solution is used, the aqueous solution is similarly made into an alkaline solution without adding a large amount of various chemical substances.

The magnetic field generator 17 generates a magnetic field B and is applied to the various amino acids A ₁ , A ₂ , A ₃ that are dissolved in the acid solution, positively charged and present in the cation state. Depending on whether the water tank 15 is rotated clockwise and the water flow v is generated clockwise, or the water tank 15 is rotated counterclockwise and the water flow v is generated counterclockwise. Determined.

The separated cylindrical amino acid simple substance layer AS ₁ , AS ₂ , AS ₃ was taken out by the amino acid take-out devices 19, 20, 21, but the separated cylindrical amino acid simple substance layer AS ₁ was removed. AS ₂ and AS ₃ may be taken out separately using the force generated from the concentration difference. In this case, the amino acid take-out device 19, 20, 21 shown in FIG. 4 omits the pumps 25, 26, 27 and the electric motors 28, 29, 30 and the suction pipes 31, 32, 33, and the discharge. The pipes 34, 35, and 36 are modified to be replaced with three resin pipes.
The method and apparatus will be described with reference to FIG. Since the amino acids A ₁ , A ₂ , A ₃ are separated into the cylindrical amino acid single layer AS ₁ , AS ₂ , AS ₃ and collected in the water tank 15, three resin pipes (not shown) are One end of the cylindrical amino acid AS ₁ , AS ₂ , AS ₃ at the respective positions r ₁ , r ₂ , r ₃ of the amino acids A ₁ , A ₂ , A ₃ , and the other end of the amino acid Each of the water tanks 15 is connected to the amino acid containers 22, 23, and 24 through the three resin pipes. Of course, at that time, the three resin pipes are filled with pure water. At this time, in the amino acid containers 22, 23, and 24, the water surface of the pure water is set lower than the height of the solution in the water tank 15. Collected is separated into its water tank 15 by doing so, and the difference of the amino acid _{A 1,} A 2, _{A 3} concentration of at present as the cylindrical amino single layer _{AS 1, AS 2, AS 3} In order to solve this problem, the pure water of the solvent is not drawn from the amino acid container 22, 23, 24 toward the water tank 15.

As such, in the amino acid container 22, 23, 24, the surface of the pure water is lower than the surface of the solution in the water tank 15, so that the amino acid container 22, 23, A force to draw the pure water of the solvent toward 24 is generated, which can counteract the force generated from the previous concentration difference. And by setting in that way, the phenomenon of trying to eliminate the concentration of amino acids A ₁ , A ₂ , A ₃ between the water tank 15 and the amino acid containers 22, 23, 24, that is, the amino acid molecule itself phenomenon occurs to flow into the amino acid container 22, 23, 24 through the three resin pipe from the water tank 15, and the amino acid _a 1 by it their _separation, a 2, _{a 3} becomes removable.

Therefore, in this amino acid separation method and separation apparatus 10, amino acids A ₁ , A ₂ , A are used by utilizing the balance between centrifugal force and Lorentz force without using a chemical reaction that adds a large amount of chemical substances. ₃ can separate each of the amino acids A ₁ , A ₂ , and A ₃ from the mixed solution as a simple substance, and a large amount of various chemical substances are added as in the case of using such a chemical reaction. The need to remove by-products derived from them is eliminated, the separation is stable and close to the in vivo environment, and the process and apparatus are simplified and simplified, and the amino acids A ₁ , A ₂ , A ₃ In addition to being able to be separated from these mixed liquids as a single unit, it is possible to effectively and efficiently separate the liquid, and as a result, the separation time is shortened and the cost is reduced. After that there separated amino acid A _1, A _2, A ₃ is as simple and amino acids A _1, which is the separation due to _its, A _2, A Effect of ₃ where is it available to obtain . As a result, this amino acid separation method and separation apparatus 10 are practical and useful.

FIG. 6 to FIG. 8 show that an aqueous solution in which a mixed solution of various amino acids A ₁ , A ₂ , A ₃ is dissolved in water is made into an acid solution, and the amino acid A ₁ , A ₂ , A _{3 is formed} from the acid solution. Another embodiment 40 of the amino acid separation method and separation apparatus of the present invention in which the amino acid is separated as a simple substance is partially shown, and this amino acid separation method and separation apparatus 40 includes the aforementioned amino acid separation method and separation method. Compared with the apparatus 10, the vertical cylindrical water tank 41 is structurally improved, and the amino acids A ₁ , A ₂ , A ₃ can be separated more effectively and efficiently from the acid solution as a single unit. It is embodied to be.

In this amino acid separation method and separation apparatus 40, the amino acid separation apparatus 40 includes a carbon dioxide bubbling device (not shown), a bowl-shaped cylindrical water tank 41, a magnetic field generation device 17, and amino acid extraction devices 19, 20, and 21. Then, the amino acid separation device 40 performs the amino acid separation method 40.
The vertical cylindrical water tank 41 is improved in a structure in which 12 partition plates 42 are added to the vertical cylindrical water tank 15 described above.
The partition plate 42 is a rectangular plate, and extends from the bottom wall 12 to the vicinity of the opening 14 inside the water tank 41 and between the cylindrical side wall 13 and the circular circular center pole 16 in the circumferential direction. Are radially arranged at predetermined intervals, and are integrally joined to the bottom wall 12, the cylindrical side wall 13, and the circular center pole 16 in cross section.
As described above, the vertical cylindrical water tank 41 has a partition plate 42 that is lower than the height of the cylindrical side wall 13 so that the partition plate 42 does not exist in the upper part of the water tank 41. Then, the acid solution is filled in the water tank 41 beyond the upper end of the partition plate 42, and when the water tank rotates, contact between the partition plate 42 and the suction pipes 31, 32, 33 is avoided. Thus, the separated amino acids A ₁ , A ₂ and A ₃ can be taken out.
Of course, the vertical cylindrical water tank 41 is rotated about the vertical axis 0 as the center 0 by the electric motor through the speed reducer, similarly to the water tank 15.

In the vertical cylindrical water tank 41, the amino acids A ₁ , A ₂ , A ₃ and the solvent water are rotated at the same speed by the effect of the partition plate 42, and the amino acids A ₁ , A ₂ , A ₃ is not affected by the flow of other water, and thus the flow velocity v is more stable than that of the tank 15, and accordingly the amino acids A ₁ , A ₂ , A ₃ are on its vertical axis 0. The cylindrical amino acid single layer AS ₁ , AS ₂ , AS ₃ is made faster, more stable and more distinct from the center 0 in the radial direction R perpendicular to each other at unique positions r ₁ , r ₂ , r ₃ . Are concentrically and concentrically formed and distributed. As such, in the water tank 41, the amino acid single layer AS ₁ , AS ₂ , AS ₃ is collected faster and with a higher density than the water tank 15 described above, and the separation time is further shortened. The Of course, this amino acid separation method and separation apparatus 40 is carried out in the same manner as the amino acid separation method and separation apparatus 10 described above, and an aqueous solution in which a mixture of various amino acids A ₁ , A ₂ , A ₃ is dissolved in water is acidified. And the amino acids A ₁ , A ₂ , A ₃ can be separated from the acid solution more effectively and more efficiently as amino acids alone.

  As will be apparent from the specific embodiments of the present invention described above with reference to the drawings, the contents of the present invention shall be understood by those skilled in the art to which the present invention pertains. Derived from the nature and nature of the, and easily embodied in another objectively recognized embodiment that makes them inherent. Of course, the content of this invention corresponds to the problem of the invention (be recommend with) and is essential for the establishment of the invention.

  As can be understood from the above, the method for separating amino acids according to the present invention is a method in which a mixed solution of a plurality of types of amino acids is preliminarily formed into an aqueous solution, and the aqueous solution is converted into an acid solution or an alkaline solution to form a cage. Put it in a cylindrical aquarium or place the aqueous solution in the aquarium and turn the aqueous solution into an acid solution or an alkaline solution in the aquarium, then rotate the aquarium around the vertical axis of the aquarium A flow of water is generated in the acid solution or alkaline solution, and a magnetic field is applied to the acid solution or alkaline solution from the upper or lower direction of the water tank in the direction of the vertical axis, or from the vertical direction, and the longitudinal direction thereof. In the radial direction perpendicular to the axis, the amino acid is located at each amino acid that balances the centrifugal force caused by the water flow and the Lorentz force caused by the magnetic field. A step of collecting concentrically as a body to form a plurality of cylindrical amino acid simple substance layers, and a step of selectively taking out the plurality of cylindrical amino acid simple substance layers or simultaneously taking them out separately. In the amino acid separation method, by using a balance between centrifugal force and Lorentz force without using a chemical reaction, first, a large amount of various chemical substances are added as in the case of using such a chemical reaction. There is no need to add a large amount of chemicals and then remove them and by-products derived from them, and multiple types of amino acids can be separated into each amino acid in a state close to the environment in the living body, and the process and equipment are more Simple and easy to implement, and its simple and simplified process and equipment enables stable separation of amino acids as a single entity In addition, it can be effectively and efficiently separated, and accordingly, the separation time is shortened and the cost is reduced.Furthermore, after separation, since the amino acid exists as a simple substance, it can be used as it is, As a result, it is useful and practical for separating amino acids.

DESCRIPTION OF SYMBOLS 10 Amino Acid Separation Method and Separator 11 Vertical Cylindrical Water Tank 12 Bottom Wall 13 Cylindrical Side Wall 14 Opening 15 Vertical Cylindrical Water Tank 16 Cross Section Circular Center Pole 17 Magnetic Field Generator 18 Electromagnetic Coil 19 Amino Acid Extractor 20 Amino Acid Extractor 20 21 Amino Acid Extraction Device 40 Amino Acid Separation Method and Separator 41 Vertical Cylindrical Water Tank 42 Partition Plate

The present invention relates to a method for separating amino acids. Specifically, at least a mixture of at least a plurality of amino acids is dissolved in water to form an aqueous solution, the aqueous solution is converted to an acid solution or an alkaline solution, and centrifugal force and Lorentz force It is related with the separation method of the amino acid which isolate | separates into each kind of the amino acid using the balance of, and takes out to each kind of amino acid.

In general, amino acid separation methods use various chemical reactions, which requires a process of adding a large amount of various chemical substances and then removing those substances and by-products derived from those substances. It was.
The general formula of the molecule of this amino acid is R—CH (NH ₂ ) COOH, and the type of amino acid varies depending on the difference in R. For example, if R is H, that is, hydrogen, it becomes H—CH (NH ₂ ) COOH, which is called glycine, and if R is CH ₃ , it becomes CH ₃ —CH (NH ₂ ) COOH, and alanine and be called.
The three-dimensional structure of this amino acid has a triangular pyramid structure. And what this amino acid peptide-bonded is protein.
This protein is hydrolyzed with a dilute acid, an alkaline solution, an enzyme (trypsin), etc., so that its peptide bond is broken and it is decomposed into individual amino acids.
Conventional amino acid separation methods generally utilize such chemical reactions.

  (Non) Patent Literature n

The object of the invention is, without the use of chemical reactions, such as addition of various amounts of chemicals can separate amino acids in a state close to the environment of the body, it separated to process and simple and easy to type of amino equipment In addition to being able to be separated stably and effectively and efficiently, the separation time can be shortened and the cost can be reduced. Is in the provision of.

Separation of amino acids of this invention, advance a plurality of types of mixture of amino acids was melt-in water solution and without, and further placed in a vertical cylindrical water tub without the aqueous solution or alkaline solution of the acid Or, the step of putting the aqueous solution into the aquarium and converting the aqueous solution into an acid solution or an alkaline solution in the aquarium, and then rotating the aquarium around the vertical axis of the aquarium to rotate the acid solution or A water flow is generated in the alkaline solution, and a magnetic field is applied to the acid solution or the alkaline solution from the upper or lower direction or the vertical direction of the water tank in the direction of the vertical axis, and the radius orthogonal to the vertical axis concentrically gathered plurality of cylindrical amino at each amino acid to balance the Lorentz force centrifugal force due to the water flow and by that the magnetic field in the direction Forming a layer, and includes the plurality of cylindrical amino layers selectively retrieve or simultaneously separately retrieve process.

Moreover, separation of amino acids of this invention, which embody allow a more simple and easy process and apparatus in advance plural kinds of amino acids mixture melt-in water to an aqueous solution and without the, and further, to the aqueous solution Bubbling carbon dioxide, making the aqueous solution into an acid solution and putting it in a vertical cylindrical water tank, then rotating the water tank around the vertical axis of the water tank to generate a water flow in the acid solution And applying a magnetic field to the acid solution simultaneously with the rotation of the aquarium or before or after the rotation of the aquarium in the direction of the longitudinal axis from the vertical direction or the vertical direction of the aquarium, and a plurality of cylindrical amino attracted concentrically at each amino acid to balance the centrifugal force and the Lorentz force due to the magnetic field due to the water flow in the radial direction perpendicular to the longitudinal axis Forming a layer, and includes the plurality of cylindrical amino layers selectively retrieve or simultaneously separately retrieve process.

The effect of the present invention is that by using a balance between centrifugal force and Lorentz force without using a chemical reaction that adds a large amount of chemical substances, first of all, as in the case of using such a chemical reaction. then there is no need to remove the by-products derived from them and to them added chemicals to large amount of added it and its various amounts of chemicals, on the type plurality kinds of amino acids is in a state close to the environment in vivo In addition to being separable, the process and equipment can be made simpler and easier and can be embodied, and the simple and simplified process and equipment makes it possible to stably separate amino acids by type. efficiently enables separated effectively, it is shortened separation time with it cost is reduced, and further, after separation, the amino acid is child present in that kind are As it is available from one to the place where it becomes possible.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a perspective view for explaining a basic concept of an amino acid separation method according to the present invention. 1 is a perspective view partially showing a specific example of an amino acid separation method and separation apparatus according to the present invention. FIG. FIG. 3 is a side view showing a partial specific example shown in FIG. 2. BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic diagram showing an overall example of an amino acid separation method and separation apparatus according to the present invention. BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic view showing an overall example of an amino acid separation method and separation device of the present invention in which an amino acid extraction device uses a force generated from a difference in concentration. FIG. 5 is a perspective view partially showing another specific example of the amino acid separation method and separation apparatus of the present invention. FIG. 7 is a side view showing another specific example shown in FIG. 6. FIG. 7 is a schematic diagram showing the other specific example shown in FIG. 6 as a whole.

Separation of amino acids of this invention is the technique of utilizing the balance of centrifugal forces and the Lorentz force, and, the embodiment of it, an aqueous solution previously melt-mixed solution of a plurality of types of amino acids in water None, and further, the aqueous solution is made into an acid solution or an alkaline solution and placed in a vertical cylindrical water tank, or the aqueous solution is put into the water tank and the aqueous solution is converted into an acid solution or an alkaline solution in the water tank. Step, and then rotating the aquarium about the longitudinal axis of the aquarium to produce a water flow in the acid or alkaline solution, and in either direction above or below the aquarium in the direction of the longitudinal axis or A magnetic field is applied to the acid solution or alkali solution from above and below, and the centrifugal force due to the water flow in the radial direction perpendicular to the longitudinal axis and the lore due to the magnetic field are applied. Step of gathering concentrically at each amino acid to balance the tool force to form a plurality of cylindrical amino layers, and includes the plurality of cylindrical amino layers selectively retrieve or simultaneously separately taken out process However, it is embodied.

Moreover, separation of amino acids of this invention, and further, carbon dioxide was bubbled into the aqueous solution, without the aqueous solution of the acid pre plural types of amino acids mixture melt-in water to an aqueous solution and without the, And then into the vertical cylindrical water tank, and then rotate the water tank around the vertical axis of the water tank to generate a water flow in the acid solution, and at the same time as the water tank rotation or the water tank Before or after rotation, a magnetic field is applied to the acid solution from above or below the water tank in the direction of the vertical axis, and the centrifugal force due to the water flow in the radial direction perpendicular to the vertical axis. and forming a plurality of cylindrical amino layers collected concentrically at each amino acid to balance the Lorentz force due to the magnetic field, and, the plurality of cylindrical Ami Including the step of taking out the acid layer selectively or simultaneously separately taken out it is embodied in simpler and easy process.

Hereinafter, based on the specific examples shown are identified, the invention illustrating the separation of the amino acid, 1 to 4, where the separation in each type from a mixture of many kinds of amino acids The specific example 10 of a separation method and a separation apparatus is shown.
First, with reference to FIG. 1, the basic idea that the method for separating amino acids of the present invention uses the balance between centrifugal force and Lorentz force will be described.
Amino acids, although the molecular formula is _{R-CH (NH 2) COOH} , in the solution of acid, positively charged becomes _-NH ^{3 +,} also in the alkaline solution, -COOH ^- negatively becomes When the solution is made either acidic or alkaline to be charged, it exists as a charged ion, ie a cation or an anion.
As shown in FIG. 1, an amino acid is previously dissolved in water to form an aqueous solution, and the aqueous solution is further made into an acid solution. The amino acid that dissolves in this acid solution is positively charged. Then, the acid solution is put into the vertical cylindrical water tank 11.
Then, as shown in FIG. 1, the vertical cylindrical water tank 11 is rotated clockwise around its longitudinal axis 0 at a predetermined rotational speed, and the water flow v is clockwise applied to the acid solution. Then, in that state, a magnetic field B is generated from the bottom of the water tank 11 in the direction of the vertical axis 0 of the water tank 11, and the magnetic field B is applied to the acid solution. Of course, this water flow v is a clockwise rotating flow.
Here, the charge of the amino acid is q (> 0), the mass is m, the magnetic field strength B, and the velocity of the water flow (velocity of amino acid molecule) is v (m / s). However, the velocity v of the water flow (water as a solvent) is slow (small) at the center of the water tank 11 and fast (large) at the inner side surface of the water tank 11.
Under such a condition, as shown in FIG. 1, the amino acid present in the radial direction R perpendicular to the vertical axis 0 of the aquarium 11, that is, at the position of the radius r from the center of the aquarium 11, The centrifugal force m · v ² / r (N) due to the water flow v and the Lorentz force q · v · B (N) due to the magnetic field B work in opposite directions, and the position where both are just balanced is r and this position is Amino acids are collected, formed and distributed in the cylindrical amino acid layer AS in the water tank 11.
This can be expressed by the equation of motion.
m · v ² / r = q · v · B (N)
Is the position (distance) where the centrifugal force and the Lorentz force are balanced, and the radius r of the distance from the center 0 of the aquarium 11 is
r = (m / q) · (v / B) (m)
Therefore, if v and B are constant, the radius r is determined by the mass m and charge q of the amino acid. That is, since amino acids have different masses m and charges q depending on the types, the positions r at which the amino acids gather in a distribution form are unique to each type of amino acid.
Utilizing this property for amino acid separation is the basic concept of the method for separating amino acids of the present invention.
Therefore, a specific example 10 of the amino acid separation method and separation apparatus of the present invention, which is embodied in a simple and simple process and apparatus, will be described.
This amino acid separation method 10 is a step of dissolving a mixed solution of various types of amino acids A ₁ , A ₂ , A ₃ in water, converting it into an acid solution, and putting it in a bowl-shaped cylindrical water tank 15. A magnetic field B is applied to the charged acid solution, that is, the various amino acids A ₁ , A ₂ , A ₃ that are dissolved in the acid solution and charged positively from the bottom of the water tank 15 upward, On the other hand, the water tank 15 is rotated clockwise about its longitudinal axis 0, and a water flow v is generated in the acid solution in the water tank 15 in the clockwise direction, and the various amino acids A ₁ , A ₂ and A ₃ are separated into a cylindrical amino acid layer AS ₁ , AS ₂ and AS ₃ in a concentric manner and collected, and the various types of cylindrical amino acid layers AS ₁ and AS ₂ . The process of taking out AS ₃ simultaneously and separately is sequentially performed, and the amino acid separation device 10 uses an electric motor (not shown) using a carbon dioxide bubbling device (not shown) and a speed reducer (not shown). 1), the vertical cylindrical water tank 15, the magnetic field generator 17, and the amino acid take-out devices 19, 20, and 21 are assembled.

In the separation method 10 of this amino acid, the step of placing a solution of the acid into its water tank 15 form the solution of the acid containing the various kinds of amino acids A _1, A _2, A ₃ in advance that many kinds of amino acids A ₁ , a _2, a mixture of a ₃ was melt-in water solution and without, and further, by bubbling carbon dioxide into the aqueous solution the solution and without of the aqueous solution the acid, and then a solution of the acid that Put in the aquarium 15. Of course, carbon dioxide is produced by the carbon dioxide bubbling device.

Solution of the multi-kinds of amino acids A _1, A _2, A ₃ collected by separating each step, within the water tank 11 is that many kinds of amino acids A _1, A _2, A ₃ is being charged positively its acid The magnetic field generator 17 applies the magnetic field B to the cation amino acid molecules A ₁ , A ₂ , A ₃ from the bottom of the water tank 15 upward, and the electric power is supplied via the speed reducer. A motor rotates the water tank 15 clockwise around the longitudinal axis 0 at a predetermined speed, causing the acid solution to flow clockwise in the water tank 15, and the longitudinal axis 0 of the water tank 15. amino acid a ₁ to balance the Lorentz force centrifugal force due to the water flow v as each type of amino acid in the radial direction R orthogonal to by its magnetic field B _in, a _2, a ₃ each radius _{1, r} 2, amino acids _A 1 that many kinds at the of _{r 3, A 2,} A ₃ collected by separating each then formed into many kinds of cylindrical amino layer _{AS 1,} AS 2, AS ₃ Distribute. Of course, in this process, even if the work of applying the magnetic field B and the work of rotating the water tank 15 are performed simultaneously, one of them is performed first, and the other is performed later. It doesn't matter.

Thus, in this step, the charge of the amino acid molecule is q (> 0) [C], the mass is m, and the magnetic field B with the magnetic flux density B (W / m ² ) is applied as shown in FIGS. When the water tank 15 is applied upward from the bottom of the water tank 15 and rotated clockwise about the longitudinal axis 0, the acid is generated by the friction force generated between the inner wall surface of the water tank 15 and the acid solution. The solution of begins to rotate clockwise.
The water as the solvent rotates in the water tank 15 at the same angular velocity from the center to the side wall in the radial direction R perpendicular to the longitudinal axis 0, so that the amino acid rotates from the center to the side wall. In order to maintain the state of movement in the direction, the water tank 15 has a circular center pole 16 having a circular cross section at the center in the center thereof and coaxially arranged on the longitudinal axis 0 thereof.

The step of separately taking out the multiple types of cylindrical amino acid layers AS ₁ , AS ₂ , AS ₃ is performed by using the plurality of amino acid take-out devices 19, 20, 21, respectively, with the multiple types of amino acid layers AS ₁ , AS ₂ , AS _3. Aspirate and remove separately. Each of the amino acid _{A 1,} A 2, _{A 3} through those steps are taken separated from a mixture of amino acids _{A 1,} A 2, _{A 3.}

The amino acid separation device 10 for performing this amino acid separation method 10 is shown in FIGS. 2 to 4 and, as described above, a carbon dioxide bubbling device (not shown), a saddle type cylinder The water tank 15, the magnetic field generator 17, and the amino acid extraction devices 19, 20, 21 are assembled.
The bowl-shaped cylindrical water tank 15 is made into a large bowl-shaped cylindrical container that is supported rotatably about the longitudinal axis 0 with the upper end open at the bottom wall 12 and the cylindrical side wall 13, and It is rotated clockwise by an electric motor (not shown) through a speed reducer (not shown).

  Further, the water tank 15 is integrally provided with a center pole 16 having a circular cross section. The center pole 16 is disposed coaxially with the longitudinal axis 0 of the water tank 15 in the water tank 15, extends from the bottom wall 12 to the opening 14, and is fixed to the bottom wall 12. . The water tank 15 does not disturb the water flow v so that the difference in the magnitude of the frictional force between the inner side surface of the cylindrical side wall 13 and the center of the water tank 15 can be ignored by the circular center pole 16 of the cross section. I am doing so.

  The magnetic field generator 17 includes an electromagnetic coil 18, a control panel (not shown), a power source (not shown), and the like. The electromagnetic coil 18 is arranged on the same axis directly below the water tank 15. Connected to the power supply at the control panel. 2, the water tank 15 is rotated clockwise as shown in FIG. 2, and the water flow v is generated clockwise. Therefore, when an electric current is applied by operating the control panel, the electromagnetic coil 18 A magnetic field B is generated from the bottom to the top. Of course, the electromagnetic coil 18 may be disposed both above and below the water tank 15 and either above or below.

As shown in FIG. 4, the amino acid take-out devices 19, 20, and 21 are amino acid containers 22, 23 and 24, pumps 25, 26 and 27, electric motors 28, 29 and 30, suction pipes 31, 32 and 33, and discharge pipes. 34, 35, 36, a control panel (not shown), a power source (not shown), etc., and the electric motors 28, 29, 30 are directly connected to the pumps 25, 26, 27 correspondingly. Connected to the power supply at the control panel.
The electric motors 28, 29, 30 are driven by the operation of the control panel, the electric motors 28, 29, 30 are driven, and the pumps 25, 26, 27 are driven by the electric motors 28, 29, 30. It is operated at 29,30. The pumps 25, 26, 27 are separated into the water tank 15, and the amino acids A ₁ , A ₂ , A ₃ collected in the cylindrical amino acid layers AS ₁ , AS ₂ , AS ₃ are collected in the amino acid containers 22, 23, 24 is sucked correspondingly and introduced.

Next, the operation of the amino acid separation apparatus 10 will be described.
In separation apparatus 10 of this amino acid, firstly, the wide variety of amino acids _{A 1,} A 2, _{A 3} mixture dissolved in water solution and pear then, carbon dioxide (CO ₂₎ was bubbled into the aqueous solution, The aqueous solution is made into an acid solution.
Thus, when the aqueous solution is made into an acid solution, the amino acids A ₁ , A ₂ , A ₃ are dissolved in the acid solution in a positively charged state, that is, in a cation state. . In this state, the acid solution is put into the water tank 15. Here, the bubbling of the carbon dioxide (CO ₂ ) is performed by the carbon dioxide bubbling apparatus (not shown). The carbon dioxide bubbling device is assembled with a number of small-bore bubbling tubes (not shown), a carbon dioxide supply source (not shown), a pump (not shown), a connection pipe (not shown), and the like.

Next, the magnetic field generator 17 is operated on its control panel, and the electromagnetic coil 18 is energized. The electromagnetic coil 18 generates a magnetic field B from the bottom of the water tank 15 upward, and the water tank 15. The magnetic field B is applied to the amino acids A ₁ , A ₂ , and A ₃ that are dissolved in the acid solution and are positively charged and exist in the cation state.
At the same time or subsequently, the water tank 15 is operated by its control panel, and the electric motor is energized to drive the electric motor. The water tank 15 is rotated clockwise around the longitudinal axis 0 by the electric motor via the speed reducer, and a water flow v is generated clockwise in the water tank 15 due to the dissolution of the acid.
As such, a magnetic field B is applied to the acid solution in the aquarium 15 and at the same time a water flow v is generated, so that the amino acids A ₁ , A ₂ , A ₃ are on the vertical axis 0 of the aquarium 15. the amino acid a ₁ at a concentrically spaced in the radial direction R _orthogonal, a _2, amino acids a ₁ balances the centrifugal force and the Lorentz force due to the magnetic field B as a cylindrical layer of a ₃ due to the water flow _v, a _2, a ₃ at the respective radii _{r 1,} r 2, _{r 3} is an amino acid _{a 1,} a 2, _{a 3} are collected each separated and formed on the cylindrical amino layer _{AS 1,} AS 2, AS ₃ Be distributed.

Each of the amino acids A ₁ , A ₂ , A ₃ is separated and collected in the water tank 15 at its radii r ₁ , r ₂ , r ₃ , and the cylindrical amino acid layers AS ₁ , AS ₂ , AS since the formed distributed _3, the cylindrical amino layer aS _{1, aS} 2, aS ₃ are separately withdrawn in its amino acid removal device 19, 20, 21.
As the amino acid extraction apparatus 19, 20, 21 are first its suction pipes 31, 32 and 33 correspond to suit the amino acid layer _{AS 1, AS 2, AS 3} , then performs an operation in which the control board The electric motors 28, 29, and 30 are energized.
Since the electric motors 28, 29, 30 are driven, the pumps 25, 26, 27 are respectively operated by the corresponding electric motors 28, 29, 30, and are separated into the water tank 15. The corresponding amino acids A ₁ , A ₂ , A ₃ formed and separated in the cylindrical amino acid layers AS ₁ , AS ₂ , AS ₃ are sucked correspondingly, and the sucked amino acids A ₁ , A ₂ , a ₃ corresponding to put led to the amino acid container 22, 23, 24.
As described above, the amino acid separation apparatus 10 is utilized, and the amino acids A ₁ , A ₂ , and A ₃ are separated into their amino acid types from the mixed solution in a state close to the environment in the living body. .

In this amino acid separation method and separation apparatus 10, carbon dioxide (CO ₂ ) is bubbled in an aqueous solution obtained by dissolving a mixture of the various types of amino acids A ₁ , A ₂ , and A ₃ in water before being transferred to the water tank 15. However, the carbon dioxide may be bubbled in the water tank 15 to form an acid solution after the aqueous solution is put in the water tank 15. In that case, it is desirable that the bubbling of the carbon dioxide is performed so as not to affect the water flow v. Further, this aqueous solution may be appropriately made into an acid solution so that the separation can be performed stably in a state close to the environment in the living body without adding a large amount of various kinds of chemical substances. On the other hand, when the alkaline solution is used, the aqueous solution is similarly made into an alkaline solution without adding a large amount of various chemical substances.

The magnetic field generator 17 generates a magnetic field B and is applied to the various amino acids A ₁ , A ₂ , A ₃ that are dissolved in the acid solution, positively charged and present in the cation state. Depending on whether the water tank 15 is rotated clockwise and the water flow v is generated clockwise, or the water tank 15 is rotated counterclockwise and the water flow v is generated counterclockwise. Determined.

The separated cylindrical amino acid layers AS ₁ , AS ₂ , AS ₃ were taken out by the amino acid take-out devices 19, 20, 21, but the separated cylindrical amino acid layers AS ₁ , AS 3 were removed. ₂ and AS ₃ may be taken out separately using the force generated from the concentration difference. In this case, the amino acid take-out device 19, 20, 21 shown in FIG. 4 omits the pumps 25, 26, 27 and the electric motors 28, 29, 30 and the suction pipes 31, 32, 33, and the discharge. The pipes 34, 35, and 36 are modified to be replaced with three resin pipes.
The method and apparatus will be described with reference to FIG. The amino acids A ₁ , A ₂ , A ₃ are separated into the cylindrical amino acid layers AS ₁ , AS ₂ , AS ₃ and collected in the water tank 15, so that three resin pipes (not shown) are One end of the amino acid A ₁ , A ₂ , A _{3 at the} respective positions r ₁ , r ₂ , r ₃ of the cylindrical amino acid layer AS ₁ , AS ₂ , AS ₃ and the other end of the amino acid container 22 , 23, and 24, and the water tank 15 is connected to the amino acid containers 22, 23, and 24 by the three resin pipes. Of course, at that time, the three resin pipes are filled with pure water. At this time, in the amino acid containers 22, 23, and 24, the water surface of the pure water is set lower than the height of the solution in the water tank 15. By doing so, the water tank 15 is separated and collected, and the amino acids A ₁ , A ₂ , A ₃ present as the cylindrical amino acid layers AS ₁ , AS ₂ , AS ₃ exhibit a difference in concentration. The pure water of the solvent is not drawn into the water tank 15 from the amino acid containers 22, 23, 24 to be eliminated.

As such, in the amino acid container 22, 23, 24, the surface of the pure water is lower than the surface of the solution in the water tank 15, so that the amino acid container 22, 23, A force to draw the pure water of the solvent toward 24 is generated, which can counteract the force generated from the previous concentration difference. And by setting in that way, the phenomenon of trying to eliminate the concentration of amino acids A ₁ , A ₂ , A ₃ between the water tank 15 and the amino acid containers 22, 23, 24, that is, the amino acid molecule itself phenomenon occurs to flow into the amino acid container 22, 23, 24 through the three resin pipe from the water tank 15, and the amino acid _a 1 by it their _separation, a 2, _{a 3} becomes removable.

Therefore, in this amino acid separation method and separation apparatus 10, amino acids A ₁ , A ₂ , A are used by utilizing the balance between centrifugal force and Lorentz force without using a chemical reaction that adds a large amount of chemical substances. ₃ can be separated from their mixture as amino acids A ₁ , A ₂ , A ₃ , respectively , and a large amount of various chemical substances are added as in the case of using such a chemical reaction. There is no need to remove the by-products derived from them, it becomes stable and separable with a bear that is close to the environment in the living body, and the amino acids A ₁ , A ₂ , A ₃ allows efficient separation and effectively in addition to allowing the separation to each kind from their mixture with it, is shortened separation time cost is reduced, and further, Amino acid A ₁ that has been separated after _release, A _2, A ₃ is present in the type, respectively, and, the separated amino acids A _1, A _2, A ₃ are directly available to become at the effects due to its Is obtained. As a result, this amino acid separation method and separation apparatus 10 are practical and useful.

FIG. 6 to FIG. 8 show that an aqueous solution in which a mixed solution of various amino acids A ₁ , A ₂ , A ₃ is dissolved in water is made into an acid solution, and the amino acid A ₁ , A ₂ , A _{3 is formed} from the acid solution. Another embodiment 40 of the amino acid separation method and separation apparatus of the present invention for separating the amino acids into their respective types is partially shown, and this amino acid separation method and separation apparatus 40 is the aforementioned amino acid separation method. and compared to the separator 10, its vertical cylindrical water tub 41 structurally improved and efficient and more effective the amino acid a _1, a _2, a ₃ in the kinds respectively from a solution of the acid It is embodied to be separable.

In this amino acid separation method and separation apparatus 40, the amino acid separation apparatus 40 includes a carbon dioxide bubbling device (not shown), a bowl-shaped cylindrical water tank 41, a magnetic field generation device 17, and amino acid extraction devices 19, 20, and 21. Then, the amino acid separation device 40 performs the amino acid separation method 40.
The vertical cylindrical water tank 41 is improved in a structure in which 12 partition plates 42 are added to the vertical cylindrical water tank 15 described above.
The partition plate 42 is a rectangular plate, and extends from the bottom wall 12 to the vicinity of the opening 14 inside the water tank 41 and between the cylindrical side wall 13 and the circular circular center pole 16 in the circumferential direction. Are radially arranged at predetermined intervals, and are integrally joined to the bottom wall 12, the cylindrical side wall 13, and the circular center pole 16 in cross section.
As described above, the vertical cylindrical water tank 41 has a partition plate 42 that is lower than the height of the cylindrical side wall 13 so that the partition plate 42 does not exist in the upper part of the water tank 41. Then, the acid solution is filled in the water tank 41 beyond the upper end of the partition plate 42, and when the water tank rotates, contact between the partition plate 42 and the suction pipes 31, 32, 33 is avoided. Thus, the separated amino acids A ₁ , A ₂ and A ₃ can be taken out.
Of course, the vertical cylindrical water tank 41 is rotated about the vertical axis 0 as the center 0 by the electric motor through the speed reducer, similarly to the water tank 15.

In the vertical cylindrical water tank 41, the amino acids A ₁ , A ₂ , A ₃ and the solvent water are rotated at the same speed by the effect of the partition plate 42, and the amino acids A ₁ , A ₂ , A ₃ is not affected by the flow of other water, and thus the flow velocity v is more stable than that of the tank 15, and accordingly the amino acids A ₁ , A ₂ , A ₃ are on its vertical axis 0. Cylindrical amino acid layers AS ₁ , AS ₂ , AS ₃ are made faster, more stable and more distinctive at the unique positions r ₁ , r ₂ , r ₃ from the center 0 in the orthogonal radial direction R, respectively. Concentric and concentrically formed and distributed. As such, in the water tank 41, the amino acid layers AS ₁ , AS ₂ , AS ₃ are collected faster and with a higher density than the water tank 15 described above, and the separation time is further shortened. . Of course, this amino acid separation method and separation apparatus 40 is carried out in the same manner as the amino acid separation method and separation apparatus 10 described above, and an aqueous solution in which a mixture of various amino acids A ₁ , A ₂ , A ₃ is dissolved in water is acidified. None of the solution and the amino acid a ₁ from a solution of the _acid, a _2, a ₃ respectively allows more efficient separation more effective for each type of amino acid.

  As will be apparent from the specific embodiments of the present invention described above with reference to the drawings, the contents of the present invention shall be understood by those skilled in the art to which the present invention pertains. Derived from the nature and essence of and readily embodied in another objectively recognized embodiment that makes them inherent. Of course, the content of this invention corresponds to the problem of the invention (be recommend with) and is essential for the establishment of the invention.

As will be appreciated from the above, separation of amino acids of this invention, advance a plurality of types of amino acids mixture melt-in water to an aqueous solution and pear and further without the aqueous solution or an alkaline solution of an acid, Or put the aqueous solution into the water tank and turn the aqueous solution into an acid solution or an alkaline solution in the water tank, and then the water tank around the longitudinal axis of the water tank. Rotate to cause a flow of water in the acid or alkaline solution, and in the direction of the longitudinal axis, apply a magnetic field to the acid or alkaline solution from either the top or bottom of the tank or from the top to bottom; and , concentrically at each amino acid to balance the Lorentz force in the radial direction perpendicular to the longitudinal axis and the centrifugal force due to the water flow by its magnetic field Forming a plurality of cylindrical amino layers collected, and, because it includes the plurality of cylindrical amino layers selectively retrieve or simultaneously separately retrieve process, separation of the amino acid of the invention, chemical By using the balance between centrifugal force and Lorentz force without using the reaction, first of all, adding a large amount of various chemical substances as in the case of using such a chemical reaction, In addition, there is no need to remove them and by-products derived from them, and multiple types of amino acids can be separated into each type of amino acid in a state close to the in vivo environment, making the process and equipment simpler and easier. becomes embodied possible Te, and in addition to the amino acid is stably separable for respective types according to simple, the simplified process and apparatus effectively Effectively allowing separation Te is reduced is shorter separation time with it the cost, and further, after separation, amino acids allows directly used since there to the type, respectively, as a result, the amino acid It is useful and practical for separation of water.

DESCRIPTION OF SYMBOLS 10 Amino Acid Separation Method and Separator 11 Vertical Cylindrical Water Tank 12 Bottom Wall 13 Cylindrical Side Wall 14 Opening 15 Vertical Cylindrical Water Tank 16 Cross Section Circular Center Pole 17 Magnetic Field Generator 18 Electromagnetic Coil 19 Amino Acid Extractor 20 Amino Acid Extractor 20 21 Amino Acid Extraction Device 40 Amino Acid Separation Method and Separator 41 Vertical Cylindrical Water Tank 42 Partition Plate

Claims (2)

An aqueous solution in which a mixed solution of a plurality of types of amino acids is dissolved in water is prepared in advance, and the aqueous solution is made into an acid solution or an alkaline solution and placed in a vertical cylindrical water tank, or the aqueous solution is put in the water tank and the water tank A step of converting the aqueous solution into an acid solution or an alkaline solution, and then rotating the water tank about the longitudinal axis of the water tank to generate a water flow in the acid solution or the alkaline solution, and A magnetic field is applied to the acid solution or alkaline solution from the top or bottom direction of the water tank in the direction of the axis, or the centrifugal force due to the water flow and the Lorentz by the magnetic field in the radial direction perpendicular to the vertical axis. Concentrically gather amino acids as single amino acids at each of the amino acids where the forces are balanced, and form multiple cylindrical amino acid single layers. To process, and the separation of the amino acid optionally include retrieving or simultaneously separately retrieve process the plurality of cylindrical amino alone layer. A step of preparing an aqueous solution in which a mixture of a plurality of amino acids is dissolved in water in advance, and further bubbling carbon dioxide into the aqueous solution, converting the aqueous solution into an acid solution and placing it in a vertical cylindrical water tank, Rotating the aquarium about the longitudinal axis of the aquarium to produce a water flow in the acid solution, and the aquarium in the direction of the longitudinal axis simultaneously with the rotation of the aquarium or before or after the rotation of the aquarium A magnetic field is applied to the acid solution from either the upper or lower direction or the vertical direction, and an amino acid is placed in each of the amino acids that balance the centrifugal force caused by the water flow and the Lorentz force caused by the magnetic field in the radial direction perpendicular to the longitudinal axis. And concentrically as a simple substance to form a plurality of cylindrical amino acid simple substance layers, and select the plurality of cylindrical amino acid simple substance layers. Separation of amino acids, including to retrieve or simultaneously separately retrieve process.

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