JP2016074610A - Expression promoting agent of filaggrin and involucrin - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an expression promoting agent of filaggrin and involucrin wherein a novel component is comprised as an active ingredient, and to provide a preventive and/or treating agent for dermatitis, such as atopic dermatitis and ichthyosis vulgaris using the same.SOLUTION: The above subject is solved by the following characteristics of the invention: (1) a filaggrin expression promoting agent containing Rosaceae Fragaria L. extract as an active ingredient; (2) the filaggrin expression promoting agent described in the above (1) wherein the extract contains tiliroside; and (3) the filaggrin expression promoting agent described in the above (1) or (2) characterized by using a seed as a site of the Rosaceae Fragaria L.SELECTED DRAWING: None

Description

  The present invention relates to an expression promoter for filaggrin and involucrin, and a dermatitis preventive and therapeutic agent using them, comprising a novel ingredient as an active ingredient.

The stratum corneum of the epidermis is located on the top surface of the skin, and functions as a moisturizer by suppressing moisture transpiration. It also functions as a barrier that suppresses the entry of microorganisms and viruses from the outside, ultraviolet rays, and invasive stimuli. So far, ceramide, a sphingolipid present in the stratum corneum, has been proposed to be important for these functions. However, recent studies have shown that other proteins also play an important role in the skin barrier function.
Filagrin is a kind of basic protein produced in epidermal granule cells, and plays an important role together with keratin in forming the stratum corneum indispensable for the barrier function of the skin. At the stage where the stratum corneum is formed by biosynthesis as the precursor profilaggrin, the profilagrin phosphate undergoes dephosphorylation and limited hydrolysis and is degraded to produce filaggrin. Profilagrin is a huge protein in which 10 to 12 filaggrins are connected. If filaggrin is not made, keratinous abnormalities occur, and the barrier function of the skin decreases, causing dermatitis. In addition, many filaggrin gene abnormalities have been found in patients with atopic dermatitis and are attracting attention as a key substance for the treatment of atopic dermatitis. Also, it is known that filaggrin expression is extremely reduced in patients with ichthyosis vulgaris.

Involucrin, on the other hand, is a highly reactive, water-soluble protein present in keratinocytes and epidermis that becomes a substrate for transglutaminase. It first appears in the cytoplasm and then undergoes cross-linking polymerization under the action of transglutaminase. Ultimately, it becomes an insoluble protein and contributes to the formation of a confined envelope. Cornified envelopes are organelles that play a major role in the keratinous barrier function. Thus, filaggrin and involucrin are important proteins in the barrier function of the skin.

On the other hand, we are conducting component studies and evaluating bioactive functions of strawberry seeds, and have clarified that the flavonoid glycosides tiliroside and kaempferol 3-O-glucoside are the main components. On the other hand, it has been found that the physiological activity shows a moisturizing action (accelerating the expression of aquaporin-3 etc. that increases the water transport function), a whitening action, and an anti-obesity action.

However, conventionally, it has been found that strawberries and extracts thereof have a preventive and therapeutic action on atopic dermatitis and dermatitis such as common ichthyosis by promoting the expression of filaggrin, involucrin and the like. Not.
Under such a background, it has been found that tiliroside and an extract of Rosaceae Fragaria L. containing them have an action of promoting the expression of filaggrin and involucrin, thereby causing atopic dermatitis and vulgaris The present invention has been completed by finding that it has an action to prevent and treat dermatitis such as ichthyosis.
That is, the present invention finds a novel use of the strawberry extract, and further promotes the expression promoter of filaggrin and involucrin containing the novel ingredient as an active ingredient, and skin such as atopic dermatitis and vulgaris ichthyosis using them. It aims at providing the prevention and treatment agent of a flame.

The features of the present invention for solving the above-described problems are as follows.
(1) A filaggrin expression promoter comprising an extract of Rosaceae Fragaria L. as an active ingredient.
(2) The filaggrin expression promoter according to (1) above, wherein the extract contains tyrilloside.
(3) The filaggrin expression promoter according to (1) or (2) above, wherein seeds are used as a part of the Rosaceae Fragaria L.
(4) A filaggrin expression promoter containing tiliroside as an active ingredient.
(5) An involucrin expression promoter comprising an extract of Rosaceae Fragaria L. as an active ingredient.
(6)
The involucrin expression promoter according to (5) above, wherein the extract contains tyrilloside.
(7) The involucrin expression promoter according to (5) or (6) above, wherein seeds are used as a part of the Rosaceae Fragaria L.
(8) An involucrin expression promoter containing tiliroside as an active ingredient.
(9) A dermatitis preventive / therapeutic agent comprising the agent according to any one of (1) to (8) above.
(10) The dermatitis preventive / therapeutic agent according to (9), wherein the dermatitis is atopic dermatitis or ichthyosis vulgaris.

It is a figure which shows the separation scheme of tiroloside. It is a graph which shows the result of having evaluated the influence which the strawberry seed extract and the influence which has on the expression of epidermal filaggrin of a tiroloside by the Western blotting method. It is a photograph which shows the result of having evaluated the effect | action which has on the expression of a strawberry seed extract and epidermal filaggrin of a tiroloside by immuno-staining. It is a graph which shows the result of having evaluated the influence which the strawberry seed extract and the influence which has on the expression of epidermis involucrin of a tiroloside by the Western blotting method. It is a photograph which shows the result of having evaluated the effect | action which has on the expression of a strawberry seed extract and epidermis involucrin of a tiroloside by immuno-staining.

The present invention is described in detail below.
The filaggrin and involucrin expression promoter of the present invention comprises an extract of Rosaceae Fragaria L. as an active ingredient.
The kind of Rosaceae Fragaria L. used as a raw material of the present invention is not particularly limited. . Rosea (Safflower snake strawberry), Fragaria nipponica var. yakusimensis (Yakushima robana snake strawberry), Fragaria nubicola, Fragaria vesca (Ezo snake strawberry) Fragaria viridis, Fragaria yezoensis (Ezoaceae strawberry), Fragaria moupinenaria, ES (Dutch strawberry), Fragaria chiloensis (Chile strawberry), Fragaria iturupensis Staudt, Fragaria virginiana (Virginia strawberry), Fragaria x Potentilla (intergeneric hybrid), Fragaria x vescana, and the like.
These may be used alone or in combination of two or more. Of these, Fragaria x ananassa DUCHESNE (Netherlands strawberry) is particularly preferable.

  The cultivar of Fragaria x ananassa DUCHESNE (Netherlands strawberry) used at this time is not particularly limited. For example, Toyono, Nyoho, Tochiotome, Sagahonoka, Akihime, Asuka Wave, Ascalbee, Iberry, Tochihime, Red Pearl, Sachinoka, Amaou, Hoso Early Childhood, Benihoppe Momoichigo, Natsumi, Summer Princess, Petica, Natsumusume (Karenya), Echigohime (Echigo) Hime) and the like, but not limited thereto. These may be used alone or in combination of two or more.

  The part of Rosaceae Fragaria L. used as a raw material is not particularly limited, but leaves, stems, fruits, flowers, seeds, etc. can be used, and seeds are particularly preferable. . This is because polyphenols such as tiliroside are contained at a high concentration, and thereby have an excellent gene expression effect for beautifying skin.

  It is preferable that tiriloside is contained as a component of Rosaceae Fragaria L .. This is because it has an excellent gene expression effect for beautiful skin. Here, tiliroside means a compound represented by the following chemical formula (1).

  At this time, the content of tiroloside is not particularly limited, but when the total mass of the expression promoter for filaggrin and involucrin is 100% by mass, 0.01-15% by mass, preferably 0.5-10% by mass. It is preferable. This is because it has a more excellent filaggrin and involucrin expression promoting action.

  In addition, the method for producing filaggrin and involucrin expression promoter is not particularly limited, but Rosaceae Fragaria L. is a seed of Rosaceae Fragaria L. In the case of using "seed"), polar solvent extraction, supercritical extraction and the like can be mentioned. In the present invention, only one of these may be used, or both of them may be used.

Here, when extracting with a polar solvent, a strawberry seed may be used as it is as an extraction raw material, but a defatted strawberry seed may be used. It is because an active ingredient can be extracted easily.
At this time, as a degreasing method, for example, the oil content may be separated by pressing strawberry seeds, or after separating the oil content, the residual oil content of the pressed product is extracted and separated with a degreasing solvent (fat-soluble organic solvent). You may do it. Furthermore, strawberry seeds may be pulverized and extracted and separated using a degreasing solvent (fat-soluble organic solvent).

  At this time, preferable degreasing solvents include n-hexane, acetone and the like. In particular, it is preferable to use n-hexane. This is because the extracted oil can be used as an edible oil and the defatted strawberry seed extract can be easily used as a food material. These degreasing solvents may be used alone or in combination of two or more.

  Furthermore, when extracting by polar solvent extraction, the polar solvent to be used is not particularly limited. For example, water, methanol, ethanol, isopropanol, acetone, 1,3-butylene glycol, ethylene glycol, propylene glycol, glycerin, acetic acid, acetic acid are used. Examples include ethyl, ether, hexane and the like. Of these, water and ethanol are preferred. In particular, it is preferable to use hydrous ethanol. These may be used alone or in combination of two or more.

  When water is used as the extraction solvent, the extraction temperature is 20 to 100 ° C., preferably about 80 to 100 ° C. This is because if the extraction temperature is too low, it is difficult to extract active ingredients. The kind of water for extraction is not particularly limited, and tap water, distilled water, mineral water, alkaline ionized water and the like can be used.

  When hydrous ethanol is used as the extraction solvent, the ethanol concentration is preferably 30 to 90 wt%. This is because if the amount is less than about 30 wt% or exceeds 90 wt%, the extraction amount of the active ingredient tends to decrease. The extraction temperature is 20 to 80 ° C, preferably about 50 to 80 ° C. In addition, the water-containing ethanol extraction may be repeated at various concentrations in order to improve the content of the active ingredient.

  In addition, when extracting with a polar solvent, any extraction method such as continuous extraction, immersion extraction, countercurrent extraction, etc. can be employed as the extraction method, and any apparatus can be used at room temperature or under reflux heating. Can do. Moreover, you may combine the said extraction method etc.

  As a specific method, an extraction raw material is put into a treatment tank filled with an extraction solvent, and an active ingredient is eluted while stirring. For example, when water or water-containing ethanol is used as the extraction solvent, the extraction is performed for about 30 minutes to 5 hours using a polar solvent of about 5 to 100 times (weight ratio) of the extraction raw material. The active ingredient is eluted in the solvent, and then filtered to remove the extraction residue to obtain an extract. Thereafter, the extract is subjected to treatments such as dilution, concentration, drying and purification according to a conventional method to obtain filaggrin and involucrin expression promoters.

  Examples of the purification method include activated carbon treatment, resin adsorption treatment, ion exchange resin, liquid-liquid countercurrent distribution, and the like, but they are not used in large quantities when added to foods. It may be used unpurified.

  Moreover, when extracting by supercritical extraction, the supercritical fluid to be used is not specifically limited, For example, a carbon dioxide, water, etc. are mentioned. In addition, these may use only 1 type and may use 2 or more types together. Of these, carbon dioxide is particularly preferred. It is because an active ingredient can be extracted more easily. The extraction method at this time may be performed by a known method.

The expression promoter for filaggrin and involucrin of the present invention is characterized by containing tyrilloside as an active ingredient.
The method for obtaining tyrilloside is not particularly limited, and can be obtained by a known method. That is, a commercially available product may be used, or it may be extracted and purified from a plant. Although the plant raw material used at this time is not specifically limited, A strawberry is preferable. When extracting and purifying from strawberry, specifically, it can be obtained by the method of Example.

  The expression promoter for filaggrin and involucrin of the present invention can be used as a material for various foods and drinks. Examples of the food and drink include edible oil (salad oil), confectionery (gum, candy, caramel, chocolate, cookie, snack, jelly, gummy, tablet confection etc.), noodles (soba, udon, ramen etc.), and dairy products ( Milk, ice cream, yogurt, etc.), seasonings (miso, soy sauce, etc.), soups, beverages (juice, coffee, tea, tea, carbonated drinks, sports drinks, etc.) and health foods (tablets , Capsules, etc.) and nutritional supplements (nutrient drinks, etc.). It is recommended that the filaggrin and involucrin expression promoter of the present invention be appropriately blended with these foods and drinks.

  These foods and drinks can be blended with various ingredients depending on the type, for example, glucose, fructose, sucrose, maltose, sorbitol, stevioside, corn syrup, lactose, citric acid, tartaric acid, malic acid, succinic acid. Acid, lactic acid, L-ascorbic acid, dl-α-tocopherol, sodium erythorbate, glycerin, propylene glycol, glycerin fatty acid ester, polyglycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, propylene glycol fatty acid ester, gum arabic, Food materials such as carrageenan, casein, gelatin, pectin, agar, vitamin Bs, nicotinic acid amide, calcium pantothenate, amino acids, calcium salts, pigments, fragrances and preservatives can be used. In addition, the filaggrin and involucrin expression promoters with health maintenance functions include other antioxidants and health food ingredients, such as antioxidants, reduced ascorbic acid (vitamin C), vitamin E. Reduced glutatin, tocotrienol, vitamin A derivative, lycopene, β-cryptoxanthin, astaxanthin, zeaxanthin, fucoxanthin, uric acid, ubiquinone, coenzyme Q10, folic acid, garlic extract, allicin, sezamin, lignans, catechin, isoflavone, chalcone, Tannins, flavonoids, coumarin, isocoumarins, blueberry extract, arbutin, tannin, anthocyanin, apple polyphenol, grape seed extract, ellagic acid, kojic acid, surge extract health food material, V. (vitamin) A, V.B1 , VB 2, V.B6, V.B12, VC, VD, VE, VP, choline, niacin, pantothenic acid, calcium folate, EPA, oligosaccharide, dietary fiber, squalene, soy lecithin, taurine, donariella, protein, octacosanol, DHA , Egg yolk lecithin, linoleic acid, lactoferrin, magnesium, zinc, chromium, selenium, potassium, heme iron, oyster meat extract, chitosan, chitin oligosaccharide, collagen, chondroitin, turmeric, licorice, kokushi, kehi, hawthorn, ginger, ganoderma , Swordfish extract, suppon, plantain, chamomile, chamomile, dandelion, hibiscus, honey, bowlen, royal jelly, lime, lavender, rosehip, rosemary, sage, bifidobacteria, faecalis, lacris, wheat germ oil, sesame oil , Soybean oil, medium Fatty acid, agaricus, ginkgo biloba extract, turmeric, chondroitin, brown rice germ extract, litchi, onion, DHA, EPA, DPA, strawberry tea, cordyceps, garlic, bee puppy, papaya, puer, propolis, megsuri tree, yabushitake, royal jelly , Saw Palmetto, Hyaluronic Acid, Collagen, Gabba, Harp Seal Oil, Shark Cartilage, Glucosamine, Lecithin, Phosphatidylserine, Tanachi Carrot, Mulberry Leaf, Soybean Extract, Echinacea, Ezoukogi, Barley Extract, Olive Leaf, Olive Seed, Gymnema , Banaba, Salacia, Garcinia, Chitosan, St. John's wort, jujube, carrot, passion flower, broccoli, placenta, pearl barley, grape seed, peanut seed coat, bilberry, black cohosh, maria thistle, laurel, sae Di, Rosemary, Rafuma, Black Vinegar, Goya, Maca, Safflower, Flax, Oolong Tea, Flower Thorn, Caffeine, Capsaicin, Xylooligosaccharide, Glucosamine, Buckwheat, Citrus, Dietary Fiber, Protein, Prunes, Spirulina, Barley Young Leaves, Nucleic Acid , Yeast, shiitake, plum meat, amino acid, deep sea potato extract, noni, oyster meat, suppon, champignon, plantain, acerola, pineapple, banana, peach, apricot, melon, strawberry, raspberry, orange, fucoidan, mesimacob, cranberry, Chondroitin sulfate, zinc, iron, ceramide, silk peptide, glycine, niacin, chesttree, ceramide, L-cysteine, L-carnitine, red wine leaf, millet, horsetail, biotin, centera asiatica, haskup, pycnogenol, fuki, Rhubarb , Clove, rosemary, catechin, puer, citric acid, brewer's yeast, merirot, black ginger, ginger, gajutsu, nattokinase, benicozi, tocotrienol, lactoferrin, cinnamon, buckwheat, cocoa, yuzu seed extract, perilla extract, lychee Seed extract, evening primrose extract, black rice extract, α-lipoic acid, gabba, fresh coffee bean extract, burdock extract, kiwi seed extract, Unshu tangerine extract, red ginger extract, leek seed extract, walnut seed extract, grape extract, kankanikuyuyo Extract, red rice extract, white jellyfish extract, lingonberry extract, kombu extract, wakame extract, rice protein, rice peptide, cherry extract, Sasa Kurihito yotake extract, plant-derived polyamine, maquiberry extract, junsai extract, Tea extract, such as chrysanthemum flower extract can also be formulated.

  As a specific production method, the filaggrin and involucrin expression promoter of the present invention can be easily spray-dried or freeze-dried together with powdered dextrin in the case of an extract, and this can be easily made into a powder, granule, tablet or solution. Can be contained in foods (instant foods, etc.). If necessary, it can also be mixed with a binder such as gum arabic to form a powder or granules, and added to a solid food. In the case of an extract, it can also be added to a beverage as it is or after being dispersed and dissolved in water, ethanol, glycerin or a mixture thereof.

  The expression promoter for filaggrin and involucrin of the present invention may be used as a raw material for drugs (including pharmaceuticals and quasi drugs). It can be produced by appropriately blending the filaggrin and involucrin expression promoter of the present invention with the raw material for the pharmaceutical preparation. In addition, the said chemical | medical agent may be used for a human and may be used for mammals other than a human. Examples of the pharmaceutical raw material that can be blended in the expression promoter for filaggrin and involucrin of the present invention include, for example, excipients (glucose, lactose, sucrose, sodium chloride, starch, calcium carbonate, kaolin, crystalline cellulose, cocoa butter, hydrogenated vegetable oil, Kaolin, talc, etc.), binder (distilled water, physiological saline, ethanol water, simple syrup, glucose solution, starch solution, gelatin solution, carboxymethylcellulose, potassium phosphate, polyvinylpyrrolidone, etc.), disintegrant (sodium alginate, agar) Sodium bicarbonate, calcium carbonate, sodium lauryl sulfate, stearic acid monoglyceride, starch, lactose, gum arabic powder, gelatin, ethanol, etc.), disintegration inhibitors (sucrose, stearin, cocoa butter, hydrogenated oil, etc.), absorption enhancers (Quaternary ammonium salt , Sodium lauryl sulfate, etc.), adsorbents (glycerin, starch, lactose, kaolin, bentonite, silicic acid, etc.), lubricants (purified talc, stearates, polyethylene glycol, and the like) and the like.

  The administration method of the filaggrin and involucrin expression promoter according to the present invention can be generally administered orally in the form of tablets, pills, soft / hard capsules, fine granules, powders, granules and the like. The water-soluble preparation can be administered orally as a liquid. Furthermore, parenteral administration may be used. When administered as a parenteral agent, the filaggrin and involucrin expression promoter of the present invention is dispersed in an appropriate solubilizing agent such as ethanol or water, and then a poultice, lotion, ointment, tincture, cream, etc. Can be applied in any dosage form. In addition, the water-soluble preparations of the filaggrin and involucrin expression promoter are used as they are or with the addition of a dispersant, suspension, stabilizer, etc. Can be applied in any dosage form.

  The dose may vary depending on the administration method, medical condition, age of the patient, etc., but for adults, it can be generally administered in an amount of about 5 to 200 mg as an active ingredient per day and usually about 0.5 to 100 mg for children.

  The compounding ratio when using the filaggrin and involucrin expression promoter of the present invention as a drug can be appropriately changed depending on the dosage form, but is usually about 0.01 when administered orally or by mucosal absorption. In the case of parenteral administration, about 0.01 to 20 wt% is recommended. In addition, since the dose varies depending on various conditions, a dose smaller than the above dose may be sufficient, or it may be necessary to administer beyond the range. The pharmaceutical composition may include, in addition to the filaggrin and involucrin expression promoters, other known compounds commonly used in the pharmaceutical field, and compounds necessary for molding into a form suitable for oral administration. . Examples of such compounds include lactose, starch, hydroxypropyl cellulose, kaolin, talc, calcium carbonate and the like.

Even when the filaggrin and involucrin expression promoter of the present invention is used as an external preparation for skin (including cosmetics, pharmaceuticals and quasi drugs), it can be expected to promote the expression of filaggrin and involucrin. In addition, the said skin external preparation may be used for a human and may be used for mammals other than a human.
Examples of the external preparation for the skin to which the expression promoter for filaggrin and involucrin of the present invention can be formulated include, for example, emulsion, soap, facial cleanser, bath preparation, cream, emulsion, lotion, eau de cologne, shave cream, shave Lotion, cosmetic oil, suntan / sunscreen lotion, powder, foundation, perfume, pack, nail cream, enamel, enamel remover, eyebrow, blusher, eye cream, eye shadow, mascara, eyeliner, lipstick, lip balm, Shampoos, rinses, hair dyes, dispersions, cleaning agents and the like can be mentioned.
Examples of the form of a pharmaceutical or quasi-drug that can contain the filaggrin and involucrin expression promoter of the present invention include ointments, creams, and liquids for external use.

In addition to the filaggrin and involucrin expression promoter according to the present invention, the external preparation for skin of the above form is blended with skin external preparations such as cosmetics and quasi-drugs as long as the promoting action of filaggrin and involucrin is not impaired. Ingredients, oils, higher alcohols, fatty acids, UV absorbers, powders, pigments, surfactants, polyhydric alcohols / sugars, polymers, bioactive ingredients, solvents, antioxidants, fragrances, preservatives, etc. be able to.
Examples are listed below, but the present invention is not limited to these examples.

(1) Examples of oil components Ester-based oil phase components: glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isostearic acid Isocetyl, butyl stearate, butyl myristate, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, adipine Diisopropyl acid, isoaralkyl neopentanoate, glyceryl tri (capryl / caprate), trimethylolpropane tri-2-ethylhexanoate, trimethylol triisostearate Propane, pentaerythritol tetra-2-ethylhexanoate, cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinoleate, isolaurate Stearyl, isotridecyl myristate, isocetyl myristate, isostearyl myristate, isocetyl palmitate, isostearyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl linoleate, octyldodecyl isoleate, isopropyl isostearate Cetostearyl 2-ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethyleneglycol dioctanoate , Ethylene glycol dioleate, propylene glycol dicaprate, propylene glycol di (capryl / capric acid), propylene glycol dicaprylate, neopentyl glycol dicaprate, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl triundecylate, triisopalmitine Glyceryl acid, glyceryl triisostearate, octyldodecyl neopentanoate, isostearyl octanoate, octyl isononanoate, hexyl decyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerin oleic acid Ester, polyglycerol isostearate, dipropyl carbonate, charcoal Dialkyl (C12-18), triisocetyl citrate, triisoaralkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyl decyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, Trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, olein Cholesteryl acid, dihydrocholesteryl oleate, phytosteryl isostearate, phytosteryl oleate, 12-stearoyl hydroxystearate Cetyl, 12-stearoyl-hydroxystearic acid stearyl 12-stearoyl-hydroxystearic acid isostearate, and the like.
Hydrocarbon oil phase components: squalane, liquid paraffin, α-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, petrolatum and the like.
Animal and vegetable oils and their hydrogenated oils, and naturally occurring waxes: beef tallow, hydrogenated beef tallow, lard, hydrogenated tallow, horse oil, hydrogenated horse oil, mink oil, orange luffy oil, fish oil, hydrogenated fish oil, egg yolk oil and other animal oils and Its hardened oil, avocado oil, almond oil, olive oil, cacao butter, kiwi seed oil, apricot oil, kukui nut oil, sesame oil, wheat germ oil, rice germ oil, rice bran oil, safflower oil, shea butter, soybean oil, evening primrose oil , Perilla oil, tea seed oil, camellia oil, corn oil, rapeseed oil, hydrogenated rapeseed oil, palm kernel oil, hydrogenated palm kernel oil, palm oil, hydrogenated palm oil, peanut oil, hydrogenated peanut oil, castor oil, hydrogenated castor oil , Vegetable oils such as sunflower oil, grape seed oil, jojoba oil, hardened jojoba oil, macadamia nut oil, medhome oil, cottonseed oil, hardened cottonseed oil, coconut oil, hardened coconut oil and its hardened , Beeswax, high acid value beeswax, lanolin, reduced lanolin, hydrogenated lanolin, liquid lanolin, carnauba wax and montan wax.
Silicone oil phase components: dimethylpolysiloxane, methylphenylpolysiloxane, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, methylhydrogenpolysiloxane, polyether-modified organopolysiloxane, dimethyl Siloxane / methylcetyloxysiloxane copolymer, dimethylsiloxane / methylstearoxysiloxane copolymer, alkyl-modified organopolysiloxane, terminal-modified organopolysiloxane, amino-modified silicone oil, amino-modified organopolysiloxane, dimethiconol, silicone gel, acrylic Examples include silicone, trimethylsiloxysilicic acid, and silicone RTV rubber.
Fluorine oil phase components: perfluoropolyether, fluorine-modified organopolysiloxane, fluorinated pitch, fluorocarbon, fluoroalcohol, fluoroalkyl / polyoxyalkylene co-modified organopolysiloxane, and the like.

(2) Examples of higher alcohols Lauryl alcohol, myristyl alcohol, cetyl alcohol, stearyl alcohol, isostearyl alcohol, oleyl alcohol, behenyl alcohol, 2-ethylhexanol, hexadecyl alcohol, octyldodecanol and the like can be mentioned.

(3) Examples of fatty acids Caprylic acid, capric acid, undecylenic acid, lauric acid, myristic acid, palmitic acid, palmitoleic acid, stearic acid, isostearic acid, oleic acid, linoleic acid, linolenic acid, arachidic acid, arachidonic acid, behenic acid , Erucic acid, 2-ethylhexanoic acid and the like.

(4) Examples of UV absorbers Paraaminobenzoic acid, amyl paraaminobenzoate, ethyldihydroxypropyl paraaminobenzoate, glyceryl paraaminobenzoate, ethyl paraaminobenzoate, octyl paraaminobenzoate, octyldimethyl paraaminobenzoate, ethylene glycol salicylate, salicylic acid Octyl, triethanolamine salicylate, phenyl salicylate, butylphenyl salicylate, benzyl salicylate, homomenthyl salicylate, benzyl cinnamate, octyl paramethoxycinnamate, 2-ethylhexyl paramethoxycinnamate, mono-2-diparamethoxycinnamate Glyceryl ethylhexanoate, isopropyl paramethoxycinnamate, diethanolamine salt of paramethoxyhydrocinnamic acid, diisopropyl diisopropylcinnamic acid Stealth mixture, urocanic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and its salts, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium disulfonate, dihydroxybenzophenone, dihydroxydimethoxybenzophenone, hydroxyoctoxybenzophenone, tetrahydroxybenzophenone, Butylmethoxydibenzoylmethane, 2,4,6-trianilino-p- (carbo-2-ethylhexyl-1-oxy) -1,3,5-triazine, 2- (2-hydroxy-5-methylphenyl) benzotriazole , Methyl-O-aminobenzoate, 2-ethylhexyl-2-cyano-3, 3-diphenyl acrylate, phenylbenzimidazole sulfate, 3- (4-methylbenzylidene) can Examples include full, isopropyldibenzoylmethane, 4- (3,4-dimethoxyphenylmethylene) -2, 5-dioxo-1-imidazolidinepropionate 2-ethylhexyl, and the like, polymer derivatives, silane derivatives, and the like.

(5) Examples of powders and pigments Red 104, Red 201, Yellow 4, Blue 1, Black 401 and other dyes, Yellow 4 AL lake, Yellow 203 BA lake and other lake dyes, nylon powder , Silk powder, urethane powder, Teflon (registered trademark) powder, silicone powder, polymethyl methacrylate powder, cellulose powder, starch, silicone elastomer spherical powder, polyethylene powder, yellow iron oxide, red iron oxide, black Colored pigments such as iron oxide, chromium oxide, carbon black, ultramarine and bitumen, white pigments such as zinc oxide, titanium oxide and cerium oxide, extender pigments such as talc, mica, sericite, kaolin and barium sulfate plate, titanium mica Pearl pigments such as barium sulfate, calcium carbonate, magnesium carbonate, aluminum silicate, metal salts such as magnesium silicate, Inorganic powders such as Rica and alumina, metal soaps such as aluminum stearate, magnesium stearate, zinc palmitate, zinc myristate, magnesium myristate, zinc laurate, zinc undecylenate, bentonite, smectite, boron nitride, etc. It is done. There are no particular restrictions on the shape of these powders (spherical, rod-like, needle-like, plate-like, irregular shape, flake-like, spindle-like, etc.) and particle diameter. These powders are conventionally known surface treatments such as fluorine compound treatment, silicone treatment, silicone resin treatment, pendant treatment, silane coupling agent treatment, titanium coupling agent treatment, oil agent treatment, N-acylated lysine treatment, The surface treatment may or may not be performed in advance by polyacrylic acid treatment, metal soap treatment, amino acid treatment, lecithin treatment, inorganic compound treatment, plasma treatment, mechanochemical treatment or the like.

(6) Examples of surfactants Anionic surfactants: fatty acid soap, α-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate , Alkylamide sulfate, alkyl phosphate, POE alkyl phosphate, alkylamide phosphate, alkyloylalkyl taurine salt, N-acyl amino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate, alkyl sulfoacetic acid Sodium, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphate, etc. are mentioned.
Cationic surfactant: alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, benzalkonium chloride , Behenic acid amidopropyldimethylhydroxypropylammonium chloride, stearic acid diethylaminoethylamide, stearic acid dimethylaminopropylamide, lanolin derivative quaternary ammonium salts, and the like.
Amphoteric surfactants: carboxybetaine type, amide betaine type, sulfobetaine type, hydroxysulfobetaine type, amide sulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type, amidoamine type and the like.
Nonionic surfactant: propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE sorbitol fatty acid ester, POE glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE cured castor Oil, POE castor oil, POE / POP copolymer, POE / POP alkyl ether, polyether-modified silicone lauric acid alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, and the like.
Natural surfactant: lecithin, saponin, sugar surfactant and the like.

(7) Examples of polyhydric alcohols and sugars Ethylene glycol, diethylene glycol, polyethylene glycol, propylene glycol, dipropylene glycol, polypropylene glycol, glycerin, diglycerin, polyglycerin, 3-methyl-1,3-butanediol, 1, 3 -Butylene glycol, sorbitol, mannitol, raffinose, erythritol, glucose, sucrose, fructose, xylitol, lactose, maltose, maltitol, trehalose, alkylated trehalose, mixed isomerized sugar, sulfated trehalose, pullulan and the like. These chemically modified compounds can also be used.

(8) Examples of polymers Acrylic ester / methacrylic acid ester copolymer (plus size, manufactured by Kyoyo Chemical), vinyl acetate / crotonic acid copolymer (resin 28-1310, manufactured by NSC), vinyl acetate / croton Acid / vinyl neodecanate copolymer (28-2930, manufactured by NSC), methyl vinyl ether maleic acid half ester (Gantrez ES, manufactured by ISP), T-butyl acrylate / ethyl acrylate / methacrylic acid copolymer (rubymer) , BASF), vinyl pyrrolidone / vinyl acetate / vinyl propionate copolymer (Rubicol VAP, BASF), vinyl acetate / crotonic acid copolymer (Rubiset CA, BASF), vinyl acetate / croton Acid / vinyl pyrrolidone copolymer (Rubiset CAP, manufactured by BASF), vinyl pyrrolidone / acrylate copolymer (Rubiflex, BA SF), acrylate / acrylamide copolymer (Ultrahold, BASF), vinyl acetate / butyl maleate / isobornyl acrylate copolymer (Advantage, ISP), carboxy vinyl polymer (Carbopol, BF Goodrich), anionic polymer compounds such as acrylic acid / alkyl methacrylate copolymer (Pemulene, BF Goodrich), and acetic acid amphoteric products of dialkylaminoethyl methacrylate polymers (Yukaformer, Mitsubishi Chemical) Amphoteric polymer compounds such as octyl acrylate acrylate / hydroxypropyl acrylate / butylaminoethyl methacrylate copolymer (AMPHOMER, NSC), quaternized vinylpyrrolidone / dimethylaminoethyl methacrylate (GAFQUAT, ISP) , Methyl vinyl imidazolium chloride Cationic polymer compounds such as vinyl / vinylpyrrolidone copolymer (rubicoat, manufactured by BASF), polyvinylpyrrolidone (rubiscol K, manufactured by BASF), vinylpyrrolidone / vinyl acetate copolymer (rubiscol VA, manufactured by BASF) ), Nonionic polymer compounds such as vinylpyrrolidone / dimethylaminoethyl methacrylate copolymer (copolymer 937, manufactured by ISP), vinylcaprolactam / vinylpyrrolidone / dimethylaminoethyl methacrylate copolymer (copolymer VC713, manufactured by ISP), etc. There is. Cellulose or derivatives thereof, keratin and collagen or derivatives thereof, calcium alginate, pullulan, agar, gelatin, tamarind seed polysaccharide, xanthan gum, carrageenan, high methoxyl pectin, low methoxyl pectin, guar gum, gum arabic, crystalline cellulose, arabino Naturally derived polymer compounds such as galactan, gum karaya, gum tragacanth, alginic acid, albumin, casein, curdlan, gellan gum and dextran can also be suitably used.

(9) Examples of physiologically active ingredients Examples of physiologically active ingredients include substances that impart some physiological activity to the skin when applied to the skin. For example, whitening ingredients, immunostimulants, anti-aging agents, UV protection agents, slimming agents, tanning agents, antioxidants, hair growth agents, hair restorers, moisturizers, blood circulation promoters, antibacterial agents, bactericides, desiccants, A cooling sensation agent, a warming sensation agent, vitamins, an amino acid, a wound healing promoter, an irritation relaxation agent, an analgesic agent, a cell activator, an enzyme component, etc. are mentioned. Examples of these suitable ingredients include, for example, ashitaba extract, avocado extract, amateur extract, altea extract, arnica extract, aloe extract, apricot extract, apricot kernel extract, ginkgo biloba extract, fennel extract, turmeric extract, oolong tea extract, ages Extract, Echinacea leaf extract, Ogon extract, Oat extract, Oen extract, Barley extract, Hypericum extract, Oyster extract, Dutch mustard extract, Orange extract, Seawater dried product, Seaweed extract, Hydrolyzed elastin, Hydrolyzed wheat powder, Hydrolyzed silk , Chamomile extract, Carrot extract, Kawara mugwort extract, Licorice extract, Calcade extract, Oyster extract, Kina extract, Cucumber extract, Guanosine, Gardenia extract, Kumazasa extract, Clarae Kiss, walnut extract, grapefruit extract, clematis extract, chlorella extract, mulberry extract, gentian extract, tea extract, yeast extract, burdock extract, fermented rice bran extract, rice germ oil, comfrey extract, collagen, bilberry extract, saicin extract, psycho extract Extract Kizuta extract, hawthorn extract, elderberry extract, yarrow extract, mint extract, sage extract, mallow extract, senki Sue extract, assembly extract, soybean extract, tiso extract, thyme extract, tea extract, clove extract, chigaya extract, chimpi extract, touki extract, toshinsen extract, tonin extract, spruce extract, dokudami extract, tomato extract, natto extract, carrot extract, garlic extract, Novara extract, hibiscus extract, bacmond extract, parsley extract, honey, hamamelis extract, parietalia extract, toad extract, bisabolol, loquat extract, dandelion extract, burdock extract, bukuro extract, butcher bloom extract, grape extract, propolis, loofah extract, Safflower extract, peppermint extract, bodaige extract, button extract, hop extract, pine extract, marronnier extract, mizubasho Kiss, Mukuroji extract, Melissa extract, Peach extract, Cornflower extract, Eucalyptus extract, Yukinoshita extract, Yokuinin extract, Artemisia extract, Lavender extract, Apple extract, Lettuce extract, Lemon extract, Lotus root extract, Rose extract, Rosemary extract, Roman chamomile extract And royal jelly extract.
Biopolymers such as deoxyribonucleic acid, mucopolysaccharide, sodium hyaluronate, sodium chondroitin sulfate, collagen, elastin, chitin, chitosan, hydrolyzed eggshell membranes, amino acids, hydrolyzed peptides, sodium lactate, urea, sodium pyrrolidonecarboxylate , Moisturizing ingredients such as betaine, whey, trimethylglycine, oily ingredients such as sphingolipid, ceramide, phytosphingosine, cholesterol, cholesterol derivatives, phospholipids, ε-aminocaproic acid, glycyrrhizic acid, β-glycyrrhetinic acid, lysozyme chloride, guaiazulene, Immunostimulants such as hydrocortisone, vitamin A, vitamin B2, vitamin B6, vitamin C, vitamin D, vitamin E, calcium pantothenate, biotin, nicotinamide, vitamin C ester Vitamins, active ingredients such as allantoin, diisopropylamine dichloroacetate, 4-aminomethylcyclohexanecarboxylic acid, antioxidants such as tocopherol, carotenoid, flavonoid, tannin, lignan, saponin, α-hydroxy acid, β-hydroxy acid, etc. Cell activators, blood circulation promoters such as γ-oryzanol and vitamin E derivatives, wound healing agents such as retinol and retinol derivatives, arbutin, kojic acid, placenta extract, sulfur, ellagic acid, linoleic acid, tranexamic acid, glutathione, etc. Whitening agent, cephalanthin, licorice extract, red pepper tincture, hinokitiol, garlic iodide extract, pyridoxine hydrochloride, DL-α-tocopherol, DL-α-tocopherol acetate, nicotinic acid, nicotinic acid derivative, calcium pantothenate, D-pan Tothenyl alcohol, acetyl pantothenyl ethyl ether, biotin, allantoin, isopropylmethylphenol, estradiol, ethinyl estradiol, capronium chloride, benzalkonium chloride, diphenhydramine hydrochloride, takanal, camphor, salicylic acid, nonyl acid vanillylamide, nonanoic acid vanillylamide, pyroctone Olamine, glyceryl pentadecanoate, L-menthol, mononitroguaiacol, resorcin, γ-aminobutyric acid, benzethonium chloride, mexiletine hydrochloride, auxin, female hormone, cantalis tincture, cyclosporine, zinc pyrithione, hydrocortisone, minoxidil, monostearic acid Examples include hair growth agents such as polyoxyethylene sorbitan, mint oil, and Sasanishiki extract.

(10) Examples of antioxidants Sodium bisulfite, sodium sulfite, erythorbic acid, sodium erythorbate, dilauryl thiodipropionate, tocopherol, tolyl biguanide, nordihydroguaiaretic acid, parahydroxyanisole, butylhydroxyanisole, dibutylhydroxy Examples include plant extracts having an antioxidant effect such as toluene, ascorbyl stearate, ascorbyl palmitate, octyl gallate, propyl gallate, carotenoid, flavonoid, tannin, lignan, saponin, apple extract and clove extract.

(11) Examples of solvents Purified water, ethanol, lower alcohol, ethers, LPG, fluorocarbon, N-methylpyrrolidone, fluoroalcohol, volatile linear silicone, next-generation chlorofluorocarbon and the like.

Example 1
Manufacture of strawberry seed extract The strawberry seed extract was prepared by crushing Polish strawberry seeds, extracting with methanol (70-80 degrees), and drying under reduced pressure. Moreover, tiliroside (trans-tiliroside) was isolated from strawberry seeds according to the following scheme. That is, 1.0 kg of strawberry (Fragaria x ananassa) seeds were extracted with methanol (5.0 L) under reflux (80 ° C) for 3 hours. The obtained extract was filtered, methanol was added to the residue, and the same extraction operation was performed 3 times in total. Thereafter, the extracts were combined and the solvent was distilled off under reduced pressure to obtain an extract (230.0 g, yield 23.0%). 230.0 g of the resulting extract was partitioned and extracted with ethyl acetate and water, and the solvent was distilled off from each transition part under reduced pressure. The ethyl acetate transition part (53.05 g, 5.3%) and the water transition part (176.95 g, 17.7% )
Part of the resulting ethyl acetate transition (25.0 g) was subjected to normal phase silica gel column chromatography (1.6 kg, CHCl ₃ -MeOH (20: 1 → 10: 1, v / v) → CHCl ₃ -MeOH-H ₂ 7 (fraction 1 (18.69 g), fraction 2 (2.17 g), fraction 3 (1.20 g), fraction separated by O (10: 3: 1 → 7: 3: 1, v / v) → MeOH) 4 (0.81 g), fraction 5 (2.29 g), fraction 6 (0.57 g), fraction 7 (0.61 g)]. A fraction (200 mg) of Fraction 3 was separated by HPLC [MeCN: H ₂ O (30:70), COSMOSIL 5C18-MS-II], and trans-tiliroside (1, 30 mg) and cis-tiliroside (2, 3.0 mg) was obtained.

Test Example 1: Evaluation of filaggrin expression promoting action Strawberry seed extract or tiliroside was added to the lower side of the membrane in a three-dimensional epidermal culture system and cultured for 5 days. During this time, the medium was changed daily. After completion of the culture, the cell layer was recovered together with the membrane. The recovered cell layer was measured for filaggrin expression by Western blotting and immunostained. The results are shown in FIG. 2 (Western blotting method) and FIG. 3 (immunostaining).

 As a result of investigating the expression of filaggrin by Western blotting, the expression of filaggrin was increased by 1 μg / mL for strawberry seed extract and 0.1 μg / mL for tiliroside (FIG. 2). In immunostaining, the expression of filaggrin was observed in the strawberry seed extract (10 μg / mL) and tiliroside (0.1 μg / mL or more) (the black-stained portion in FIG. 3).

Strawberry seed extract or tiliroside was added to the lower side of the membrane in the three-dimensional epidermal culture system and cultured for 5 days. During this time, the medium was changed daily. After completion of the culture, the cell layer was recovered together with the membrane. The recovered cell layer was measured for involucrin expression by Western blotting and immunostained.

The expression of involucrin, which plays an important role in keratin maturation, was examined by Western blotting. As a result, strawberry seed extract increased the expression of filaggrin at 1 μg / mL and tiliroside at 0.1 μg / mL or higher (Figure 4). In immunostaining, both strawberry seed extract and tiroloside showed enhanced expression in the stratum corneum (the darkened area in Fig. 5). From the above results, it was found that strawberry seed extract and its main component, tiroloside, have the effect of preventing and treating dermatitis by increasing the expression level of filaggrin and involucrin.

The compounding example of the expression promoter (strawberry seed extract) of the filaggrin and involucrin of a present Example is shown. These do not limit the present invention.
Formulation Example 1: Cosmetic cream
Squalane 20.0wt%
Beeswax 5.0
Refined jojoba oil 5.0
Glycerin 5.0
Glycerol monostearate 2.0
Polyoxyethylene (20) sorbitan-
Monostearate 2.0
Strawberry seed extract 2.0
Preservative appropriate amount
Perfume
Purified water residue
100.0 wt%

Formulation Example 2: Lotion
Ethanol 5.0wt%
Glycerin 2.0
1,3-butylene glycol 2.0
Polyethylene oleyl ether 0.5
Sodium citrate 0.1
Citric acid 0.1
Strawberry seed extract 0.1
Purified water residue
100.0 wt%

Formulation Example 3: Body Gel
Macadamia nut oil 2.0wt%
Octyldodecyl myristate 10.0
Methylphenylpolysiloxane 5.0
Behenyl alcohol 3.0
Stearic acid 3.0
Batyl alcohol 1.0
Glyceryl monostearate 1.0
Tetraoleic acid polyoxyethylene sorbit 2.0
Hydrogenated soybean phospholipid 1.0
Ceramide 0.1
Retinol palmitate 0.1
Preservative appropriate amount
Centella extract 1.0
Strawberry seed extract 1.0
1,3-butylene glycol 5.0
Purified water residue
100.0 wt%

Formulation Example 4: Latex
Squalane 4.0wt%
Vaseline 2.5
Cetanol 2.0
Glycerin 2.0
Lipophilic glyceryl monostearate 1.0
Stearic acid 1.0
L-Arginine 1.0
Strawberry seed extract 0.5
Potassium hydroxide 0.1
Perfume
Purified water residue
100.0wt%

Formulation Example 5: Bath agent (liquid)
Propylene glycol 50.0wt%
Ethanol 20.0
Sodium sulfate 5.0
Strawberry seed extract 0.5
Lanolin 0.5
Avocado oil 0.5
Dye 1.5
Fragrance 22.0
100.0wt%
Formulation Example 6: Chewing gum
53.0wt% sugar
Gum base 20.0
Glucose 10.0
Minamata 16.0
Fragrance 0.5
Strawberry seed extract 0.5
100.0 wt%

Formulation Example 7: Gummy
Reduced water tank 40.0wt%
Granulated sugar 20.0
Glucose 20.0
Gelatin 4.7
Water 9.68
Strawberry juice 4.0
Grape flavor 0.6
Dye 0.02
Strawberry seed extract 1.0
100.0 wt%

Formulation Example 8: Candy
50.0 wt% sugar
Minamata 33.0
Water 14.4
Organic acid 2.0
Fragrance 0.2
Strawberry seed extract 0.4
100.0 wt%

Formulation Example 9: Yogurt (Hard / Soft)
Milk 41.5wt%
Nonfat dry milk 5.8
Sugar 8.0
Agar 0.15
Gelatin 0.1
Lactic acid bacteria 0.005
Strawberry seed extract 0.4
Perfume
Water residue
100.0 wt%

Formulation Example 10: Soft drink
Fructose glucose liquid sugar 30.0wt%
Emulsifier 0.5
Strawberry seed extract 0.05
Perfume
Purified water residue
100.0 wt%

Formulation Example 11: Tablets
76.4 wt% sugar
Glucose 19.0
Sucrose fatty acid ester 0.2
Strawberry seed extract 0.5
Purified water 3.9
100.0 wt%

Formulation Example 12: Soft capsule
Strawberry seed oil 87.0wt%
Emulsifier 12.0
Strawberry seed extract 1.0
100.0 wt%

Formulation Example 13: Tablet
Lactose 54.0wt%
Crystalline cellulose 30.0
Starch degradation product 10.0
Glycerin fatty acid ester 5.0
Strawberry seed extract 1.0
100.0 wt%

Formulation Example 14: Oral granules (pharmaceuticals)
Strawberry seed extract 1.0wt%
Lactose 30.0
Cornstarch 60.0
Crystalline cellulose 8.0
Polyvinylpyrrolidone 1.0
100.0 wt%

Formulation Example 15: Cat food
Corn 34.0wt%
Flour 35.0
Meat meal 15.0
Beef tallow 8.9
Salt 1.0
Skipjack extract 4.0
Strawberry seed extract 1.0
Taurine 0.1
Vitamins 0.5
Minerals 0.5
100.0 wt%

Formulation Example 16: Dog food
Corn 30.0wt%
Meat (chicken) 15.0
Defatted soybean 10.0
Flour 25.0
Mosses 5.0
Strawberry seed extract 5.0
Animal fats and oils 8.9
Oligosaccharide 0.1
Vitamin 0.5
Mineral 0.5
100.0 wt%

As described above, the present invention can provide an excellent dermatitis preventive / therapeutic agent by increasing the expression level of filaggrin or involucrin.

Claims (10)

A filaggrin expression promoter comprising an extract of Rosaceae Fragaria L. as an active ingredient. The filaggrin expression promoter according to claim 1, wherein the extract contains tyrilloside. 3. The filaggrin expression promoter according to claim 1 or 2, wherein seeds are used as a part of the Rosaceae genus (Rosaceae Fragaria L.). A filaggrin expression promoter containing tyriloside as an active ingredient. An involucrin expression promoter containing an extract of Rosaceae Fragaria L. as an active ingredient. The involucrin expression promoter according to claim 5, wherein the extract contains tyrilloside. The involucrin expression promoter according to claim 5 or 6, wherein seeds are used as a part of the Rosaceae Fragaria L .. Involucrin expression promoter containing tyriloside as an active ingredient. A dermatitis prevention / treatment agent comprising the agent according to any one of claims 1 to 8. The dermatitis preventive / therapeutic agent according to claim 9, wherein the dermatitis is atopic dermatitis or ichthyosis vulgaris.