JP2011105652A - Skin-beautifying composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a skin-beautifying composition including a new component inhibiting expression of hyaluronidase gene, ceramidase gene and collagenase gene, thereby preventing aging of the skin. <P>SOLUTION: A hyaluronidase gene expression inhibitor, a ceramidase gene expression inhibitor and a collagenase gene expression inhibitor each includes an extract of Citrus junos seed. The skin-beautifying composition includes the hyaluronidase gene expression inhibitor, the ceramidase gene expression inhibitor or the collagenase gene expression inhibitor. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

The present invention relates to a skin beautifying composition. The present invention is widely used for foods, pharmaceuticals, cosmetics and the like.

  Hyaluronic acid retains moisture in the interstitial space, retains cells based on the formation of a jelly-like matrix in the tissue, retains tissue lubricity and flexibility, resists external forces such as mechanical failure, and It has many functions such as prevention of bacterial infection.

On the other hand, hyaluronic acid staining intensity between epidermal cells decreases due to aging, and photoelastic fibrosis caused by ultraviolet irradiation (solar
It has been reported that almost no hyaluronic acid in the part where elastosis has occurred is detected, and as a result, it is thought to cause dryness of the skin, firmness, decreased elasticity, and consequently increased wrinkles. In order to improve such a state, a method of keeping the skin surface moisturized by applying a cosmetic containing hyaluronic acid has been taken, but since hyaluronic acid, which is a polymer, does not penetrate the skin, it is fundamentally Improvement cannot be expected. Therefore, it is expected to develop a substance that fundamentally improves the skin function by enhancing the hyaluronic acid synthesizing ability inherent in the cell itself.

  In addition, a component generally referred to as ceramide is sphingolipid, glycosphingolipid, or a mixture thereof. Generally speaking, sphingolipid is the most abundant component in lipids constituting human skin stratum corneum and is said to occupy about 40 to 50% or more of stratum corneum intercellular lipids. It plays an important role in moisturizing the skin and is known to have a skin protecting effect and an effect of preventing rough skin, and thereby, cosmetics and foods containing ceramide have been developed.

  In addition, preventing and improving wrinkles and sagging that occur with aging is an important cosmetic issue. Wrinkles and sagging are remarkable in areas exposed to sunlight, and the phenomenon in which wrinkles and sagging occur in the skin exposed to sunlight is called photoaging. It is known that the amount of collagen in the dermis is remarkably reduced in photoaged wrinkles. On the other hand, collagen accounts for 90% or more of the dermal matrix, and it is also known that degradation is enhanced by collagenase activated by ultraviolet rays. From these facts, it is considered that the reduction of collagen in the skin is the main cause of wrinkling and sagging of the skin. Therefore, if an agent capable of suppressing the decrease in collagen can be provided, not only photoaging but also wrinkles and sagging associated with aging can be prevented and improved.

Therefore, suppressing the production of hyaluronidase, ceramidase, collagenase, and the like by suppressing the expression of genes such as hyaluronidase, ceramidase, collagenase, etc. is important for giving skin elasticity and elasticity and preventing skin aging. Therefore, several gene expression inhibitors such as hyaluronidase, ceramidase, collagenase and the like have been developed for the purpose of preventing skin aging.

Under such a background, the present inventors have found that a Yuzu extract suppresses the expression of hyaluronidase, ceramidase, and collagenase genes, thereby completing the present invention.
That is, an object of the present invention is to provide a skin-beautifying composition having a novel component that can suppress hyaluronidase, ceramidase, and collagenase gene expression and thereby prevent skin aging.

The features of the present invention for solving the above-described problems are as follows.
1. A hyaluronidase gene expression inhibitor comprising a yuzu seed extract as an active ingredient.
2. A ceramidase gene expression inhibitor comprising a yuzu seed extract as an active ingredient.
3. Collagenase gene expression inhibitor comprising a yuzu seed extract as an active ingredient.
4). Above 1. 2. The hyaluronidase gene expression inhibitor described in 2. above, Or the ceramidase gene expression inhibitor described in 3. above, or 3. A composition for beautifying skin comprising the collagenase gene expression inhibitor described in 1.

It is a graph which shows the cytotoxicity (n = 1) of a present Example.

Hereinafter, the present invention will be described in detail.
The skin-beautifying composition of the present invention is characterized by comprising a yuzu extract as an active ingredient.
As a raw material for extracting the composition for skin beautification according to the present invention, yuzu fruits, fruit juices, pericarps, fungi and the like can be used, and seeds are particularly preferred.

Solvents for extracting active ingredients from Yuzu include n-hexane, ether, acetone, ethyl acetate, methanol, ethanol, isopropyl alcohol, butanol, 1,3-butylene glycol, ethylene glycol, propylene glycol, glycerin, and water Etc. can be used. Two or more of these solvents may be mixed. Alternatively, supercritical extraction with carbon dioxide gas may be used.
Desirably, when water or ethanol is used as the extraction solvent, the active ingredient is efficiently extracted. In particular, hydrous ethanol is an extraction solvent that is less likely to reduce the activity of the active ingredient during extraction and is preferable from the standpoint of safety in the use of the extract in food. The kind of water for extraction is not particularly limited, and tap water, distilled water, mineral water, alkaline ionized water, deep water and the like can be used.

  As the extraction temperature for extracting the yuzu seed solvent extract, for example, when water-containing ethanol is used, the extraction temperature is 20 to 80 ° C., preferably about 50 to 80 ° C. This is because if the extraction temperature is too low, it becomes difficult to extract the active ingredient, and if the extraction temperature is too high, the activity of the active ingredient tends to decrease.

The water-containing ethanol as the extraction solvent has an ethanol concentration of 10 to 90% (wt / wt), desirably an ethanol concentration of 60 to 80% (wt / wt). The reason why the ethanol concentration is 60% (wt / wt) or more is that when the ethanol content is too small, the extraction amount of the active ingredient tends to be insufficient. In addition, in order to improve the content rate of an active ingredient, it is good to perform ethanol extraction repeatedly, changing ethanol concentration in steps.

As a method for extracting functional components derived from these seeds, any method such as continuous extraction, immersion extraction, countercurrent extraction, and supercritical extraction can be employed, and any apparatus can be used at room temperature or under heating. Can do.

A specific method for extracting a Yuzu seed solvent extract is as follows: an extraction raw material Yuzu seed) is introduced into a treatment tank filled with the extraction solvent, and the active ingredient is eluted while stirring. The raw material for extraction may be used as it is, but if it is pulverized in advance, the extraction efficiency is good. For example, when water-containing ethanol is used as the extraction solvent, the extraction solvent is used in an amount of about 5 to 100 times (weight ratio) of the extraction raw material, and extraction is performed for about 30 minutes to 2 hours. The active ingredient is eluted in the solvent, and then filtered to remove the extraction residue to obtain an extract. Thereafter, the extract is subjected to treatments such as dilution, concentration, drying, purification and the like according to a conventional method to obtain the skin beautifying composition according to the present invention.
Examples of the purification method include activated carbon treatment, resin adsorption treatment, ion exchange resin, liquid-liquid countercurrent distribution, and the like.

  The skin beautifying composition of the present invention suppresses the expression of a hyaluronidase gene that is an enzyme that degrades hyaluronic acid. Thereby, since the production | generation of hyaluronidase is suppressed, decomposition | disassembly of a hyaluronic acid is suppressed, thereby, it can prevent the drying of a skin, a firmness, a fall of elasticity, and an increase in wrinkles.

  Moreover, the composition for beautiful skin of this invention suppresses the gene expression of ceramidase which is an enzyme which decomposes | disassembles ceramide. Thereby, the reduction | decrease of the ceramide in a body is suppressed, and it has the effect of moisturizing and protecting the skin and preventing rough skin.

  Furthermore, the skin beautifying composition of the present invention suppresses the expression of collagenase, an enzyme that degrades collagen. Thereby, a decrease in collagen in the body can be suppressed, and therefore, photoaging of the skin can be prevented, and wrinkles and sagging associated with aging can be prevented and improved.

The skin beautifying composition of the present invention can be used as a material for various foods and drinks. Examples of food and drink include edible oils (salad oil, confectionery (gum, candy, caramel, chocolate, cookies, snacks, jelly, gummi, tablet confectionery, etc.), noodles (soba, udon, ramen, etc.), dairy products (milk , Ice cream, yogurt, etc.), seasonings (miso, soy sauce, etc.), soups, beverages (juice, coffee, tea, tea, carbonated drinks, sports drinks, etc.) and health foods (tablets, Capsules, etc.), nutritional supplements (nutrient drinks, etc.) The skin-beautifying composition of the present invention may be appropriately blended with these foods and drinks.

These foods and drinks can be blended with various ingredients depending on the type, for example, glucose, fructose, sucrose, maltose, sorbitol, stevioside, corn syrup, lactose, citric acid, tartaric acid, malic acid, succinic acid. Acid, lactic acid, L-ascorbic acid, dl-α-tocopherol, sodium erythorbate, glycerin, propylene glycol, glycerin fatty acid ester, polyglycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, propylene glycol fatty acid ester, gum arabic, Food materials such as carrageenan, casein, gelatin, pectin, agar, vitamin Bs, nicotinic acid amide, calcium pantothenate, amino acids, calcium salts, pigments, fragrances and preservatives can be used. Furthermore, the present skin care composition having a health maintenance function includes other antioxidants and health food materials such as reduced ascorbic acid (vitamin C), vitamin E, reduced glutatin, tocotrienol, vitamin A derivatives. , Lycopene, β-cryptoxanthin, astaxanthin, zeaxanthin, fucoxanthin, uric acid, ubiquinone, coenzyme Q10, folic acid, garlic extract, allicin, sezamin, lignans, catechin, isoflavone, chalcone, tannins, flavonoids, coumarin, isocoumarins , Blueberry extract, health food material) (Vitamin) A, V. B1, V. B2, V.B. B6, V.I. B12, V.I. C, V.D. D, V.D. E, V.E. P, choline, niacin, pantothenic acid, calcium folate, EPA, oligosaccharide, dietary fiber, squalene, soy lecithin, taurine, donariella, protein, octacosanol, DHA, egg yolk lecithin, linoleic acid, lactoferrin, magnesium, zinc, chromium, selenium , Potassium, heme iron, oyster meat extract, chitosan, chitin oligosaccharide, collagen, chondroitin, turmeric, licorice, kokushi, keihi, hawthorn, ginger, ganoderma, swordfish extract, suppon, licorice, kukoshi, keihi, hawthorn, ginger, Ganoderma, plantain, chamomile, chamomile, dandelion, hibiscus, honey, boren, royal jelly, lime, lavender, rosehip, rosemary, sage, bifidobacteria, faecalis, lacris, wheat germ oil Sesame oil, perilla oil, soybean oil, medium chain fatty acid, agaricus, ginkgo biloba extract, turmeric, chondroitin, brown rice germ extract, litchi, onion, DHA, EPA, DPA, green tea, cordyceps, garlic, bee, papaya, puer, Propolis, Meguri Tree, Yabushake, Royal Jelly, Saw Palmetto, Hyaluronic Acid, Collagen, Gabba, Harp Seal Oil, Shark Cartilage, Glucosamine, Lecithin, Phosphatidylserine, Seven Carrots, Mulberry Leaf, Soybean Extract, Echinacea, Ezoukogi, Barley Extract, Olive leaf, Olive fruit, Gymnema, Banaba, Salacia, Garcinia, Chitosan, St. John's wort, Jujube, Carrot, Passion flower, Broccoli, Placenta, Barley, Grape seed, Peanut seed coat, Bilberry, Bra Kukohosh, Maria Thistle, Laurel, Sage, Rosemary, Luffa, Black Vinegar, Goya, Maca, Safflower, Flax, Oolong Tea, Flower Thorn, Caffeine, Capsaicin, Xylooligosaccharide, Glucosamine, Buckwheat, Citrus, Dietary Fiber, Protein, Prunes , Spirulina, Barley Young Leaves, Nucleic Acid, Yeast, Shiitake Mushroom, Plum Meat, Amino Acid, Deep Sea Mushroom Extract, Noni, Oyster Meat, Suppon, Champignon, Plantain, Acerola, Pineapple, Banana, Peach, Apricot, Melon, Strawberry, Raspberry, Orange , Fucoidan, Meshimakobu, Cranberry, Chondroitin sulfate, Zinc, Iron, Ceramide, Silk peptide, Glycine, Niacin, Chesttree, Ceramide, L-cysteine, Red wine confectionery, Millet, Horsetail, Biotin, Centella Asiatica, Haskup, Pic Nogenol, Japanese cypress, rhubarb, clove, rosemary, catechin, puer, citric acid, brewer's yeast, merilot, black zinger, ginger, gadget, nattokinase, benichouji, tocotrienol, lactoferrin, cinnamon, buckwheat, cocoa, etc. it can.

As a specific production method, a yuzu seed solvent extract is spray-dried or freeze-dried together with powdered cellulose, and this is easily made into powders, granules, tablets, or solutions to be included in foods and drinks (instant foods, etc.) Can do. In addition, the yuzu seed solvent extract can be dissolved in, for example, fats and oils, ethanol, glycerin, or a mixture thereof to form a liquid and added to a beverage or added to a solid food. If necessary, it can be mixed with a binder such as gum arabic or dextrin to form a powder or granules and added to a beverage or a solid food.

As the addition amount when applying the skin-beautifying composition of the present invention to foods and drinks, since the main purpose is disease prevention and health maintenance, the content of active ingredients is 1 to 20 wt% or less in total for the foods and drinks. Preferably there is.

The skin beautifying composition of the present invention may be used as a raw material for medicines (including pharmaceuticals and quasi drugs). It can be manufactured by appropriately blending the skin beautifying composition of the present invention with a raw material for a pharmaceutical preparation. Examples of the preparation raw material that can be blended in the skin beautifying composition of the present invention include excipients (glucose, lactose, sucrose, sodium chloride, starch, calcium carbonate, kaolin, crystalline cellulose, cocoa butter, hydrogenated vegetable oil, kaolin, talc, and the like. Etc.), binder (distilled water, physiological saline, ethanol water, simple syrup, glucose solution, starch solution, gelatin solution, carboxymethylcellulose, potassium phosphate, polyvinylpyrrolidone, etc.), disintegrant (sodium alginate, agar, hydrogen carbonate) Sodium, calcium carbonate, sodium lauryl sulfate, stearic acid monoglyceride, starch, lactose, gum arabic powder, gelatin, ethanol, etc.), disintegration inhibitors (sucrose, stearin, cocoa butter, hydrogenated oil, etc.), absorption enhancers (fourth) Grade ammonium base, sodium lauryl sulfate, etc.) Adsorbent (glycerin, starch, lactose, kaolin, bentonite, silicic acid, etc.), lubricants (purified talc, stearates, polyethylene glycol, and the like) and the like.

The method for administering the skin beautifying composition according to the present invention can be generally administered orally in the form of tablets, pills, soft / hard capsules, fine granules, powders, granules, liquids, etc. It may be administered parenterally. When administered as a parenteral agent, it can be applied in the form of a solution, or in the form of a haptic agent, lotion agent, ointment, tincture, cream, etc. with the addition of a dispersant, suspension, stabilizer, etc. can do.

The dosage may vary depending on the administration method, medical condition, age of the patient, etc., but for adults, it can be generally administered as 0.5 to 5000 mg as an active ingredient per day, and usually about 0.5 to 3000 mg for children.
The blending ratio of the beautifying skin composition can be appropriately changed depending on the dosage form, but is usually about 0.3 to 15.0 wt% when administered orally or by mucosal absorption, and when administered parenterally. , About 0.01 to 10 wt%. In addition, since the dose varies depending on various conditions, a dose smaller than the above dose may be sufficient, or it may be necessary to administer beyond the range.

Examples of the form of the external skin material that can be formulated with the skin beautifying composition of the present invention include, for example, emulsion, soap, face wash, bath preparation, cream, emulsion, lotion, eau de cologne, shaving cream, shaving lotion, Cosmetic oil, suntan / sunscreen lotion, powder, foundation, perfume, pack, nail cream, enamel, enamel remover, eyebrow, blusher, eye cream, eye shadow, mascara, eyeliner, lipstick, lip balm, shampoo, rinse , Hair dyes, dispersions, cleaning agents and the like.
Examples of the form of a pharmaceutical or quasi-drug that can be formulated with the skin beautifying composition of the present invention include ointments, creams, and liquids for external use.

In addition to the skin beautifying composition according to the present invention, the external preparation for skin of the above-described form includes ingredients, oils, higher alcohols, and other ingredients blended in skin external preparations such as cosmetics and quasi-drugs, as long as the skin beautifying effect is not impaired. Fatty acids, ultraviolet absorbers, powders, pigments, surfactants, polyhydric alcohols / sugars, polymers, physiologically active ingredients, solvents, antioxidants, fragrances, preservatives, and the like can be blended.
Examples are listed below, but the present invention is not limited to these examples.
(1) Examples of oil components Ester-based oil phase components: glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isostearic acid Isocetyl, butyl stearate, butyl myristate, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, adipine Diisopropyl acid, isoaralkyl neopentanoate, glyceryl tri (capryl / capric acid), trimethylolpropane tri-2-ethylhexanoate, triisostearic acid tri Tyrololpropane, pentaerythritol tetra-2-ethylhexanoate, cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinoleate, lauric acid Isostearyl, isotridecyl myristate, isocetyl myristate, isostearyl myristate, isocetyl palmitate, isostearyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl linoleate, octyldodecyl isoleate, isopropyl isostearate , Cetostearyl 2-ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, diocta Ethylene glycol acid, ethylene glycol dioleate, propylene glycol dicaprate, propylene glycol di (capryl / capric acid), propylene glycol dicaprylate, neopentyl glycol dicaprate, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl triundecylate, tri Glyceryl isopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostearyl octoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerin Oleic acid ester, polyglycerol isostearic acid ester, charcoal Dipropyl acid, dialkyl carbonate (C12-18), triisocetyl citrate, triisoaralkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyl triethyl citrate, citrate Acetyl tributyl acid, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, hydroxystearic acid Cholesteryl acid, cholesteryl oleate, dihydrocholesteryl oleate, phytosteryl isostearate, phytosteryl oleate, 12-sulfate Aroyl hydroxystearic acid isocetyl, 12-stearoyl-hydroxystearic acid stearyl 12-stearoyl-hydroxystearic acid isostearate, and the like.
Hydrocarbon oil phase components : squalane, liquid paraffin, α-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, petrolatum and the like.
Animal and vegetable oils and their hydrogenated oils, and naturally occurring waxes: beef tallow, hydrogenated beef tallow, lard, hydrogenated tallow, horse oil, hydrogenated horse oil, mink oil, orange luffy oil, fish oil, hydrogenated fish oil, egg yolk oil and other animal oils Its hardened oil, avocado oil, almond oil, olive oil, cacao butter, apricot oil, kukui nut oil, sesame oil, wheat germ oil, rice germ oil, rice bran oil, safflower oil, shea butter, soybean oil, evening primrose oil, perilla oil, Tea seed oil, camellia oil, corn oil, rapeseed oil, hardened rapeseed oil, palm kernel oil, hardened palm kernel oil, palm oil, hardened palm oil, peanut oil, hardened peanut oil, castor oil, hardened castor oil, sunflower oil, Vegetable oil such as grape seed oil, jojoba oil, hardened jojoba oil, macadamia nut oil, medhome oil, cottonseed oil, hardened cottonseed oil, coconut oil, hardened coconut oil and its hardened oil, beeswax, Acid number beeswax, lanolin, reduced lanolin, hydrogenated lanolin, liquid lanolin, carnauba wax and montan wax.
Silicone oil phase components : dimethylpolysiloxane, methylphenylpolysiloxane, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, methylhydrogenpolysiloxane, polyether-modified organopolysiloxane, dimethyl Siloxane / methylcetyloxysiloxane copolymer, dimethylsiloxane / methylstearoxysiloxane copolymer, alkyl-modified organopolysiloxane, terminal-modified organopolysiloxane, amino-modified silicone oil, amino-modified organopolysiloxane, dimethiconol, silicone gel, acrylic Examples include silicone, trimethylsiloxysilicic acid, and silicone RTV rubber.
Fluorine oil phase components: perfluoropolyether, fluorine-modified organopolysiloxane, fluorinated pitch, fluorocarbon, fluoroalcohol, fluoroalkyl / polyoxyalkylene co-modified organopolysiloxane, and the like.
(2) Examples of higher alcohols include lauryl alcohol, myristyl alcohol, cetyl alcohol, stearyl alcohol, isostearyl alcohol, oleyl alcohol, behenyl alcohol, 2-ethylhexanol, hexadecyl alcohol, octyldodecanol and the like.
(3) Examples of fatty acids Caprylic acid, capric acid, undecylenic acid, lauric acid, myristic acid, palmitic acid, palmitoleic acid, stearic acid, isostearic acid, oleic acid, linoleic acid, linolenic acid, arachidic acid, arachidonic acid, behenic acid , Erucic acid, 2-ethylhexanoic acid and the like.
(4) Examples of UV absorbers Paraaminobenzoic acid, amyl paraaminobenzoate, ethyldihydroxypropyl paraaminobenzoate, glyceryl paraaminobenzoate, ethyl paraaminobenzoate, octyl paraaminobenzoate, octyldimethyl paraaminobenzoate, ethylene glycol salicylate, salicylic acid Octyl, triethanolamine salicylate, phenyl salicylate, butylphenyl salicylate, benzyl salicylate, homomenthyl salicylate, benzyl cinnamate, octyl paramethoxycinnamate, 2-ethylhexyl paramethoxycinnamate, mono 2-diparamethoxycinnamate Glyceryl ethylhexanoate, isopropyl paramethoxycinnamate, diethanolamine salt of paramethoxyhydrocinnamate, diisopropyl diisopropylke Cinnamic acid ester mixture, urocanic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and its salt, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium disulfonate, dihydroxybenzophenone, dihydroxydimethoxybenzophenone, hydroxyoctoxybenzophenone, Tetrahydroxybenzophenone, butylmethoxydibenzoylmethane, 2,4,6-trianilino-p- (carbo-2-ethylhexyl-1-oxy) -1,3,5-triazine, 2- (2-hydroxy-5-methyl) Phenyl) benzotriazole, methyl-O-aminobenzoate, 2-ethylhexyl-2-cyano-3,3-diphenyl acrylate, phenol Rubenzimidazole sulfate, 3- (4-methylbenzylidene) camphor, isopropyldibenzoylmethane, 4- (3,4-dimethoxyphenylmethylene) -2,5-dioxo-1-imidazolidinepropionate 2-ethylhexyl, and the like, and These polymer derivatives, silane derivatives and the like can be mentioned.
(5) Examples of powders and pigments: Red No. 104, Red No. 201, Yellow No. 4, Blue No. 1, Black No. 401 and other dyes, Yellow No. 4 AL lake, Yellow No. 203 BA lake and other lake dyes, Nylon powder , Silk powder, urethane powder, Teflon (registered trademark) powder, silicone powder, polymethyl methacrylate powder, cellulose powder, starch, silicone elastomer spherical powder, polyethylene powder, yellow iron oxide, red iron oxide, black Colored pigments such as iron oxide, chromium oxide, carbon black, ultramarine and bitumen, white pigments such as zinc oxide, titanium oxide and cerium oxide, extender pigments such as talc, mica, sericite, kaolin and barium sulfate plate, titanium mica Pearl pigments such as barium sulfate, calcium carbonate, magnesium carbonate, aluminum silicate, silica Metal salts such as magnesium, inorganic powders such as silica and alumina, metal soap such as aluminum stearate, magnesium stearate, zinc palmitate, zinc myristate, magnesium myristate, zinc laurate, zinc undecylenate, bentonite, smectite And boron nitride. There are no particular restrictions on the shape of these powders (spherical, rod-like, needle-like, plate-like, irregular shape, flake-like, spindle-like, etc.) and particle diameter. These powders are conventionally known surface treatments such as fluorine compound treatment, silicone treatment, silicone resin treatment, pendant treatment, silane coupling agent treatment, titanium coupling agent treatment, oil agent treatment, N-acylated lysine treatment, The surface treatment may or may not be performed in advance by polyacrylic acid treatment, metal soap treatment, amino acid treatment, lecithin treatment, inorganic compound treatment, plasma treatment, mechanochemical treatment or the like.
(6) Examples of surfactants
Anionic surfactants : fatty acid soap, α-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkyl amide sulfate, alkyl Phosphate, POE alkyl phosphate, alkylamide phosphate, alkyloyl alkyl taurine salt, N-acyl amino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate, alkyl sulfoacetate sodium, acylated hydrolyzed collagen Peptide salts, perfluoroalkyl phosphate esters and the like can be mentioned.
Cationic surfactant : alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, benzalkonium chloride , Behenic acid amidopropyldimethylhydroxypropylammonium chloride, stearic acid diethylaminoethylamide, stearic acid dimethylaminopropylamide, lanolin derivative quaternary ammonium salts, and the like.
Amphoteric surfactants : carboxybetaine type, amide betaine type, sulfobetaine type, hydroxysulfobetaine type, amide sulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type, amidoamine type and the like.
Nonionic surfactant : propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE sorbit fatty acid ester, POE glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE cured castor Oil, POE castor oil, POE / POP copolymer, POE / POP alkyl ether, polyether-modified silicone lauric acid alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid and the like.
Natural surfactant : lecithin, saponin, sugar surfactant and the like.
(7) Examples of polyhydric alcohols and sugars Ethylene glycol, diethylene glycol, polyethylene glycol, propylene glycol, dipropylene glycol, polypropylene glycol, glycerin, diglycerin, polyglycerin, 3-methyl-1,3-butanediol, 1, 3 -Butylene glycol, sorbitol, mannitol, raffinose, erythritol, glucose, sucrose, fructose, xylitol, lactose, maltose, maltitol, trehalose, alkylated trehalose, mixed isomerized sugar, sulfated trehalose, pullulan and the like. These chemically modified compounds can also be used.
(8) Examples of polymers Acrylic ester / methacrylic acid ester copolymer (plus size, manufactured by Kyoyo Chemical), vinyl acetate / crotonic acid copolymer (resin 28-1310, manufactured by NSC), vinyl acetate / croton Acid / vinyl neodecanate copolymer (28-2930, manufactured by NSC), methyl vinyl ether maleic acid half ester (Gantrez ES, manufactured by ISP), T-butyl acrylate / ethyl acrylate / methacrylic acid copolymer (rubymer) , Manufactured by BASF), vinylpyrrolidone / vinyl acetate / vinyl propionate copolymer (rubiscol VAP, manufactured by BASF), vinyl acetate / crotonic acid copolymer (ruby set CA, manufactured by BASF), vinyl acetate / croton Acid / vinyl pyrrolidone copolymer (ruby set CAP, BA F), vinyl pyrrolidone / acrylate copolymer (Rubiflex, BASF), acrylate / acrylamide copolymer (Ultrahold, BASF), vinyl acetate / butyl maleate / isobornyl acrylate copolymer Anionic polymer compounds such as coalescence (Advantage, made by ISP), carboxyvinyl polymer (Carbopol, made by BFGoodrich), acrylic acid / alkyl methacrylate copolymer (Pemulene, made by BFGoodrich), and dialkylaminoethyl methacrylate Amphoteric products such as amphoteric products of polymers (Yuka Former, manufactured by Mitsubishi Chemical), octyl acrylate acrylate / hydroxypropyl acrylate / butylaminoethyl methacrylate copolymer (AMPHOMER, manufactured by NSC), etc. Molecular compounds, cationic polymer compounds such as quaternized vinylpyrrolidone / dimethylaminoethyl methacrylate (GAFQUAT, manufactured by ISP), methylvinylimidazolium chloride / vinylpyrrolidone copolymer (ruby coat, manufactured by BASF), polyvinylpyrrolidone (Lubicol K, manufactured by BASF), vinylpyrrolidone / vinyl acetate copolymer (Rubicol VA, manufactured by BASF), vinylpyrrolidone / dimethylaminoethyl methacrylate copolymer (copolymer 937, manufactured by ISP), vinyl caprolactam / Nonionic polymer compounds such as vinylpyrrolidone / dimethylaminoethyl methacrylate copolymer (copolymer VC713, manufactured by ISP) and the like. Cellulose or derivatives thereof, keratin and collagen or derivatives thereof, calcium alginate, pullulan, agar, gelatin, tamarind seed polysaccharide, xanthan gum, carrageenan, high methoxyl pectin, low methoxyl pectin, guar gum, gum arabic, crystalline cellulose, arabino Naturally derived polymer compounds such as galactan, gum karaya, gum tragacanth, alginic acid, albumin, casein, curdlan, gellan gum and dextran can also be suitably used.
(9) Examples of physiologically active ingredients Examples of physiologically active ingredients include substances that impart some physiological activity to the skin when applied to the skin. For example, whitening ingredients, anti-inflammatory agents, anti-aging agents, UV protection agents, slimming agents, tanning agents, antioxidants, hair growth agents, hair restorers, moisturizers, blood circulation promoters, antibacterial agents, bactericides, desiccants, A cooling sensation agent, a warming sensation agent, vitamins, an amino acid, a wound healing promoter, an irritation relaxation agent, an analgesic agent, a cell activator, an enzyme component, etc. are mentioned. Examples of these suitable ingredients include, for example, ashitaba extract, avocado extract, amateur extract, altea extract, arnica extract, aloe extract, apricot extract, apricot kernel extract, ginkgo biloba extract, fennel extract, turmeric extract, oolong tea extract, ages Extract, Echinacea leaf extract, Ogon extract, Oat extract, Oen extract, Barley extract, Hypericum extract, Oyster extract, Dutch mustard extract, Orange extract, Seawater dried product, Seaweed extract, Hydrolyzed elastin, Hydrolyzed wheat powder, Hydrolyzed silk , Chamomile extract, carrot extract, cormorant extract, licorice extract, calcade extract, oyster extract, kiwi extract, kina extract, cucumber extract, guanosine, gardenia extract, kumazasa Kiss, Clara extract, Walnut extract, Grapefruit extract, Clematis extract, Chlorella extract, Mulberry extract, Gentian extract, Black tea extract, Yeast extract, Burdock extract, Rice bran ferment extract, Rice germ oil, Comfrey extract, Collagen, Cowberry extract, Saisin extract , Psycho extract, Saitai extract, Salvia extract, Soybean extract, Sasa extract, Hawthorn extract, Salamander extract, Shiitake extract, Giant extract, Shikon extract, Perilla extract, Linden extract, Shimotake extract, Japanese peony extract, Ginger root extract, Birch extract, Japanese cedar Extract, Kizuta extract, Hawthorn extract, Elderberry extract, Achillea millefolium extract, Pepper extract, Sage extract, Zenia A extract, sensu extract, assembly extract, soybean extract, thymus extract, thyme extract, tea extract, clove extract, chigaya extract, chimpi extract, toki extract, toukisenka extract, tonin extract, spruce extract, dokudami extract, tomato extract, natto extract, carrot extract, Garlic extract, Novara extract, Hibiscus extract, Bacmond extract, Parsley extract, Honey, Camelis extract, Parietalia extract, Butterberry extract, Bisabolol, Biwa extract, Japanese dandelion extract, Fukinoto extract, Bukcho extract, Butcher bloom extract, Grape extract, Propolis, Loofah extract, safflower extract, peppermint extract, bodaiju extract, button extract, hop extract, pine extract, Maroni Eki , Mizuba pepper extract, Mukuroji extract, Melissa extract, Peach extract, Cornflower extract, Eucalyptus extract, Yukinoshita extract, Yokuinin extract, Mugwort extract, Lavender extract, Apple extract, Lettuce extract, Lemon extract, Astragalus extract, Rose extract, Rosemary extract, Roman chamomile extract, royal jelly extract and the like.
Biopolymers such as deoxyribonucleic acid, mucopolysaccharide, sodium hyaluronate, sodium chondroitin sulfate, collagen, elastin, chitin, chitosan, hydrolyzed eggshell membranes, amino acids, hydrolyzed peptides, sodium lactate, urea, sodium pyrrolidonecarboxylate , Moisturizing ingredients such as betaine, whey, trimethylglycine, oily ingredients such as sphingolipid, ceramide, phytosphingosine, cholesterol, cholesterol derivatives, phospholipids, ε-aminocaproic acid, glycyrrhizic acid, β-glycyrrhetinic acid, lysozyme chloride, guaiazulene, Anti-inflammatory agents such as hydrocortisone, vitamin A, vitamin B2, vitamin B6, vitamin C, vitamin D, vitamin E, calcium pantothenate, biotin, nicotinamide, vitamin Vitamins such as C-ester, active ingredients such as allantoin, diisopropylamine dichloroacetate, 4-aminomethylcyclohexanecarboxylic acid, antioxidants such as tocopherol, carotenoid, flavonoid, tannin, lignan, saponin, α-hydroxy acid, β -Cell activators such as hydroxy acids, blood circulation promoters such as γ-oryzanol and vitamin E derivatives, wound healing agents such as retinol and retinol derivatives, arbutin, kojic acid, placenta extract, sulfur, ellagic acid, linoleic acid, tranexamic acid , Whitening agents such as glutathione, cephalanthin, licorice extract, red pepper tincture, hinokitiol, garlic iodide extract, pyridoxine hydrochloride, DL-α-tocopherol, DL-α-tocopherol acetate, nicotinic acid, nicotinic acid derivatives , Calcium pantothenate, D-pantothenyl alcohol, acetyl pantothenyl ethyl ether, biotin, allantoin, isopropylmethylphenol, estradiol, ethinylestradiol, capronium chloride, benzalkonium chloride, diphenhydramine hydrochloride, takanal, camphor, salicylic acid, vanillylamide nonyl , Nonanoic acid vanillylamide, piroctone olamine, glyceryl pentatecanate, L-menthol, mononitroguaiacol, resorcin, γ-aminobutyric acid, benzethonium chloride, mexiletine hydrochloride, auxin, female hormone, cantharis tincture, cyclosporine, zinc pyrithione, hydrocol Thizone, minoxidil, polyoxyethylene sorbitan monostearate, peppermint oil, Sasanishikie Hair tonic, such as the scan and the like.
(10) Examples of antioxidants: sodium bisulfite, sodium sulfite, erythorbic acid, sodium erythorbate, dilauryl thiodipropionate, tocopherol, tolylbiguanide, nordihydroguaiaretic acid, parahydroxyanisole, butylhydroxyanisole, dibutylhydroxy Examples include toluene, ascorbyl stearate, ascorbyl palmitate, octyl gallate, propyl gallate, carotenoids, flavonoids, tannins, lignans, saponins, plant extracts having an antioxidant effect such as apple extract and clove extract.
(11) Examples of solvents Purified water, ethanol, lower alcohol, ethers, LPG, fluorocarbon, N-methylpyrrolidone, fluoroalcohol, volatile straight-chain silicone, next-generation chlorofluorocarbon and the like.

Examples of the present invention will be described below. In addition, the Example shown below is demonstrated in order to confirm the skin beautifying action etc. of the composition obtained by this invention, and the scope of the present invention is not limited to these products and manufacturing methods.

[Production of skin beautifying composition]
Yuzu used as a raw material was made in Japan. First, yuzu seeds were pulverized and extracted with n-hexane at 60 ° C. for 1 hour. This was filtered, and the residue was extracted with 70% ethanol at 70 ° C. for 4 hours. After filtration, the filtrate was distilled under reduced pressure. Furthermore, what was vacuum-dried and made into a powder form was used as a yuzu seed solvent extract (limonin content: 1.4%).

[Evaluation of Composition for Beautiful Skin]
Method: Using a human three-dimensional cultured epidermis model (manufactured by J-TEC), the effect of the yuzu seed solvent extract of this example was evaluated. It added to the dermis layer side so that the final concentration might be 1,10 and 100 mg / mL. The medium was changed every 2 days and cultured for 7 days. Cytotoxicity in the MTT assay was evaluated and the expression of each gene of hyaluronidase (HYAL2, HYAL3), ceramidase (Ceramidase, SM Synthase), and collagenase (MMP1) was evaluated. The results are shown in Table 1 (HYAL2, HYAL3), Table 2 (Ceramidase, SM Synthase), and Table 3 (MMP1).

Results and Effects of Examples As a result of the experiment, cytotoxicity was not observed with 1-100 mg / mL of Yuzu seed solvent extract (Fig. 1).
According to Table 1, it was confirmed that the expression of the gene of hyaluronidase, which is a hyaluronic acid-degrading enzyme, is suppressed by adding a yuzu seed solvent extract. In particular, it was confirmed that HYAL3 suppresses the expression of the hyaluronidase gene within a wide range of 1 to 100 mg / mL. Thereby, decomposition | disassembly of a hyaluronic acid is suppressed and it can prevent the drying of a skin, elasticity, the fall of elasticity, and the increase in wrinkles as a result.
Moreover, according to Table 2, it was confirmed that the expression of the ceramidase gene is suppressed at 1 mg / mL of the yuzu seed solvent extract. Thereby, it can be understood that the yuzu seed solvent extract has an effect of protecting the skin and preventing rough skin because it suppresses the decomposition of ceramide in the skin.
Furthermore, according to Table 3, it turns out that the expression of a collagenase gene is suppressed in a yuzu seed solvent extract 1-10 mg / mL. Thereby, the reduction | decrease of collagen can be suppressed, the photoaging of skin can be suppressed, and also wrinkles and sagging accompanying aging can be prevented and improved.

The compounding example of the composition for beautiful skin (Yuzu seed solvent extract) by this invention is shown.
Formulation Example 1: Chewing gum
53.0wt% sugar
Gum base 20.0
Glucose 10.0
Minamata 16.0
Fragrance 0.5
Beautiful skin composition 0.5
100.0 wt%

Formulation Example 2: Gummy
Reduced water tank 40.0wt%
Granulated sugar 20.0
Buto sugar 20.0
Gelatin 4.7
Water 9.68
Yuzu fruit juice 4.0
Yuzu Flavor 0.6
Dye 0.02
Skin care composition 1.0
100.0 wt%

Formulation Example 3: Candy
50.0 wt% sugar
Minamata 33.0
Water 14.4
Organic acid 2.0
Fragrance 0.2
Skin care composition 0.4
100.0 wt%

Formulation Example 4: Yogurt (hard / soft)
Milk 41.5wt%
Nonfat dry milk 5.8
Sugar 8.0
Agar 0.15
Gelatin 0.1
Lactic acid bacteria 0.005
Skin care composition 0.4
Perfume
Water residue
100.0 wt%

Formulation Example 5: Soft drink
Fructose glucose liquid sugar 30.0wt%
Emulsifier 0.5
Skin care composition 0.3
Perfume
Purified water residue
100.0 wt%

Formulation Example 6: Tablets
76.4 wt% sugar
Glucose 19.0
Sucrose fatty acid ester 0.2
Beautiful skin composition 0.5
Purified water 3.9
100.0 wt%

Formulation Example 7: Soft capsule
Brown rice germ oil 47.0wt%
Yuzu seed oil 40.0
Emulsifier 12.0
Skin care composition 1.0
100.0 wt%

Formulation Example 8: Tablet
Lactose 54.0wt%
Crystalline cellulose 30.0
Starch degradation product 10.0
Glycerin fatty acid ester 5.0
Skin care composition 1.0
100.0 wt%

Formulation Example 9: Cosmetic cream
Squalane 20.0wt%
Beeswax 5.0
Refined jojoba oil 5.0
Glycerin 5.0
Glycerol monostearate 2.0
Polyoxyethylene (20) sorbitan
Monostearate 2.0
Skin care composition 2.0
Preservative appropriate amount
Perfume
Purified water residue
100.0 wt%

Formulation Example 10: lotion
Ethanol 5.0wt%
Glycerin 2.0
1,3-butylene glycol 2.0
Polyethylene oleyl ether 0.5
Sodium citrate 0.1
Citric acid 0.1
Skin care composition 0.1
Purified water residue
100.0 wt%

Formulation Example 11: Body gel
Macadamia nut oil 2.0wt%
Octyldodecyl myristate 10.0
Methylphenylpolysiloxane 5.0
Behenyl alcohol 3.0
Stearic acid 3.0
Batyl alcohol 1.0
Glyceryl monostearate 1.0
Tetraoleic acid polyoxyethylene sorbit
2.0
Hydrogenated soybean phospholipid 1.0
Ceramide 0.1
Retinol palmitate 0.1
Preservative appropriate amount
Camellia extract 1.0
Skin care composition 1.0
1,3-butylene glycol 5.0
Purified water residue
100.0 wt%

Formulation Example 12: Emulsion
Squalane 4.0wt%
Vaseline 2.5
Cetanol 2.0
Glycerin 2.0
Lipophilic glyceryl monostearate
1.0
Stearic acid 1.0
L-Arginine 1.0
Beautiful skin composition 0.5
Potassium hydroxide 0.1
Fragrance
Purified water residue
100.0 wt%

Formulation Example 13: Bath agent (liquid)
Propylene glycol 50.0wt%
Ethanol 20.0
Sodium sulfate 5.0
Beautiful skin composition 0.5
Lanolin 0.5
Avocado oil 0.5
Dye 1.5
Fragrance 22.0
100.0 wt%

  As described above, the present invention can provide a skin-beautifying composition having a novel component that can suppress the expression of collagenase, ceramidase, and hyaluronidase, thereby preventing skin aging.

Claims (4)

A hyaluronidase gene expression inhibitor comprising a yuzu seed extract as an active ingredient. A ceramidase gene expression inhibitor comprising a yuzu seed extract as an active ingredient. Collagenase gene expression inhibitor comprising a yuzu seed extract as an active ingredient. A composition for beautifying skin comprising the hyaluronidase gene expression inhibitor according to claim 1, the ceramidase gene expression inhibitor according to claim 2, or the collagenase gene expression inhibitor according to claim 3.

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