JP2016061585A - Blood coagulation detection device, blood coagulation detection method, and blood coagulation detection program - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a blood coagulation detection device that can detect coagulation of blood only by irradiating the blood stored in a container with light without mechanical operations on the container, thus reducing a time to detection.SOLUTION: A blood coagulation detection device 100 comprises: a light source 3 for irradiating measured blood BL stored in a blood collection tube (a container) 1 with light; a light receiver 5 for receiving the transmission light through the measured blood BL; an absorbancy calculation part 8 for calculating absorbancy over a predetermined wavelength region according to the received transmission light; and a coagulation detection part 9 for performing secondary differential on the absorbancy at the wavelength and detecting coagulation of the measured blood BL in the blood collection tube 1 according to the measured secondary differential absorbancy, which is a result of the secondary differential.SELECTED DRAWING: Figure 1

Description

  The present invention relates to a blood coagulation detection device, a blood coagulation detection method, and a blood coagulation detection program for detecting coagulation of blood collected using absorbance.

  Conventionally, in blood tests, for example, an appropriate amount of blood collected using a vacuum blood collection tube (hereinafter referred to as a blood collection tube) is dispensed from the blood collection tube into a cuvette of the blood test apparatus. Sorting is performed by inserting a nozzle into the blood collection tube and sucking the blood in the blood collection tube with the tip of the nozzle reaching the blood. At this time, if the blood in the blood collection tube is coagulated, the nozzle clogs because the coagulated portion in the blood, that is, blood clot, is sucked into the nozzle. In this way, if the nozzle is clogged at the time of sorting, it becomes impossible to sort at that time, and many blood tests will be forced to stop, so that the blood in the blood collection tube is coagulated Must be detected for each blood collection tube before blood is collected.

  Whether or not the blood contained in the blood collection tube has been coagulated has been manually determined before, but in recent years, various methods and apparatuses have been considered. For example, in Patent Document 1, blood inside a container is imaged, blood is moved in the container by rotating the container by a predetermined angle in the direction from the vertical posture to the horizontal, and blood is imaged in a state where the blood is moved. Then, the area of the image before and after the rotation of the specimen container is calculated, and the coagulation of blood is determined based on the difference between the obtained areas.

  Further, in Patent Document 2, the container is rotated until the bottom of the container is positioned above the lid of the container, and held in that state. The held container is imaged, and the blood liquid in the container in the captured image is captured. Coagulation of blood is determined based on the presence or absence of aggregates protruding from the surface.

Japanese Patent No. 3957864 Japanese Patent No. 5139912

  However, in Patent Document 1 and Patent Document 2, the container must be rotated by a predetermined angle before solidification is detected, which complicates the apparatus configuration and the inspection process. In addition, the time required to rotate a large number of containers containing the blood to be examined and the time required to image them one by one are necessarily required, so it took time to detect the total number of coagulations. . As a result, as the number of containers to be examined increases, the possibility that blood will clot before the examination occurs may increase.

  As described above, the reason for detecting the presence or absence of coagulation in the end by tilting or rotating the container is that the components of coagulated blood and non-coagulated blood are substantially the same. This is because, in the optical detection method such as analysis, there is no difference between the two optical spectra, and it is considered impossible to detect coagulation.

  In contrast, as a result of intensive studies, the inventors of the present invention have found that blood coagulation can be detected from a secondary differential spectroscopic spectrum by subjecting the absorbance to a second derivative with respect to wavelength and performing spectroscopic analysis.

  The present invention has been made in view of the above-described problems, and blood coagulation can be detected only by irradiating light to blood stored in a container without adding mechanical operation to the container. An object of the present invention is to provide a blood coagulation detecting device capable of shortening the time.

  That is, the blood coagulation detection apparatus according to the present invention is based on a light source that irradiates light to measurement blood stored in a container, a light receiver that receives transmitted light that has passed through the measurement blood, and the received transmitted light. An absorbance calculation unit for calculating the absorbance over a predetermined wavelength range, and second-order differentiation of the absorbance with respect to the wavelength, and detecting the coagulation of the measurement blood in the container based on the second-order differential absorbance as a result of the second-order differentiation And a coagulation detector.

  According to such a configuration, the light that the light emitted from the light source passes through the measurement blood in the container and is received by the light receiver, and the absorbance calculated by the absorbance calculation unit over a predetermined wavelength region based on the transmitted light. Is second-order differentiated by the wavelength in the coagulation detector, and blood coagulation is detected based on the measured second-order differential absorbance which is the second derivative result. It is possible to detect absorbance peaks that cannot be detected by the absorbance calculated by the absorbance calculation unit by second-order differentiation of the absorbance, and as a result, blood coagulation can be performed based on such absorbance peaks. It becomes possible to detect.

  In order to calculate the absorbance with high accuracy, the absorbance calculation unit includes a transmittance calculation unit that calculates the transmittance of the transmitted light, and an absorbance calculation unit that calculates the absorbance based on the calculated transmittance. The coagulation detection unit is predetermined from a differential operation unit that secondarily differentiates the absorbance with a wavelength, and a second derivative absorbance based on the measured second derivative absorbance (hereinafter referred to as a reference second derivative absorbance). It is desirable to include a coagulation determination unit that determines that the measured blood is coagulated when the measured blood is more than the threshold value.

  In order to reduce the influence of noise, the absorbance calculation unit further includes an average value calculation unit that calculates a moving average value for the wavelength of the absorbance, and the differential calculation unit uses the moving average value as a wavelength. It is desirable to use a second derivative.

  In order to quickly detect blood coagulation, the coagulation detector detects blood coagulation when the measured second derivative absorbance is more than a predetermined threshold from the reference second derivative absorbance at wavelengths of 750 to 1050 nm. What to do is desirable. In the specific wavelength range of 750 to 1050 nm, the second derivative spectrum of uncoagulated blood is stable without significant change, whereas the second derivative spectrum of coagulated blood changes greatly. Therefore, coagulation can be detected easily and accurately. Moreover, even when a label is attached to the container, the light in the specific wavelength region is preferable because it passes through the label.

  The blood coagulation detection method according to the present invention includes an irradiation step of irradiating light stored in a container with light, a light receiving step of receiving transmitted light transmitted through the blood, and an absorbance based on the received transmitted light. And a clotting detection step of performing a second-order differentiation of the absorbance with respect to wavelength and performing spectral analysis, and detecting coagulation of blood in the container based on the analysis result.

  As described above, according to the blood coagulation detection device of the present invention, since the second derivative spectroscopy is employed, blood coagulation can be detected only by irradiating light, and therefore a mechanism for tilting or vibrating the container. This eliminates the need for a process and makes it possible to promote compactness and simplification of the apparatus and to quickly detect blood coagulation.

The block diagram which shows the whole structure of embodiment of this invention. 2 is an exemplary block diagram showing the configuration of the information processing apparatus of the embodiment. FIG. The spectral spectrum figure of the 2nd derivative absorbance in each of the blood which has not coagulated and the blood coagulated of the embodiment. The 1st modification which shows typically arrangement | positioning of the light source and light receiver with respect to the blood-collecting tube in embodiment of this invention. The 2nd modification which shows typically arrangement | positioning of the light source and light receiver with respect to the blood-collecting tube in embodiment of this invention. The 3rd modification which shows typically arrangement | positioning of the light source and light receiver with respect to the blood-collecting tube in embodiment of this invention. The 4th modification which shows typically arrangement | positioning of the light source and light receiver with respect to the blood-collecting tube in embodiment of this invention. The 5th modification which shows typically arrangement of a light source and a photoreceiver to a blood collection tube in an embodiment of the present invention.

  An embodiment of the present invention will be described with reference to the drawings.

  The blood coagulation detecting device 100 shown in FIGS. 1 to 2 includes a support base 2 that supports a blood collection tube 1 that is a container containing measurement blood BL, and a blood collection tube 1 that is supported by the support base 2. A light source 3 that irradiates light to the measurement blood BL stored therein, a spectroscope 4 that splits the transmitted light that has passed through the blood collection tube 1, a light receiver 5 that receives the transmitted light dispersed by the spectroscope 4, and light reception And an information processing device 6 for calculating the absorbance based on the intensity signal of the transmitted light output from the vessel 5 and detecting the coagulation of the measured blood BL based on the calculation result.

  The support base 2 includes a concave portion 2a that is long in the vertical direction in the center portion, and includes a light source side opening 2b and a light receiving side opening 2c that are provided at positions facing and communicating with the concave portion 2a. The support base 2 supports the blood collection tube 1 by inserting the blood collection tube 1 into the recess 2a. In a state where the blood collection tube 1 is supported, the concave portion 2a is prevented from entering light from the outside. In this embodiment, the light source side opening 2b and the light receiving side opening 2c are provided on the same straight line with the recess 2a interposed therebetween. In addition, in the state where the support 2 supports the blood collection tube 1 and the measurement blood BL coagulates, as shown in FIG. 1, considering that the clot CL sinks to the lower part of the support tube 1, The light source side opening 2b and the light receiving side opening 2c are provided corresponding to the lower part of the recess 2a. The light source side opening 2b transmits light emitted from the light source 3, that is, primary light. The light-receiving side opening 2c allows the transmitted light that has passed through the measurement blood BL to pass therethrough.

  The light source 3 is, for example, a halogen lamp that irradiates the measurement blood BL with primary light including at least infrared light through the light source side opening 2b.

  The spectroscope 4 separates the transmitted light from the light receiving side opening 2c for each wavelength, and includes, for example, a prism, a diffraction grating, and a slit.

  The light receiver 5 outputs a current or a voltage corresponding to the intensity of transmitted light for each wavelength dispersed by the spectroscope 4, and is a sensor such as a photodiode, a phototransistor, or a CCD having a sensitivity distribution up to near infrared rays. is there.

  The information processing apparatus 6 includes a dedicated or general-purpose computer including a CPU 6a, a memory 6b, an input / output interface 6c, a display 6d, a keyboard 6e, a mouse 6f, and the like. An absorbance calculation unit 8 that calculates the absorbance over a predetermined wavelength range based on the transmitted light for each wavelength received by the photoreceiver 5, and second-order differentiation of the absorbance with respect to the wavelength, and a second-order differential measurement result A coagulation detector 9 for detecting coagulation of the measurement blood BL in the blood collection tube 1 based on the absorbance is provided. The predetermined wavelength range is, for example, 500 nm to 1100 nm.

  The absorbance calculation unit 8 calculates a transmittance calculation unit 8a that calculates the transmittance of transmitted light, an absorbance calculation unit 8b that calculates an absorbance for each wavelength based on the calculated transmittance, and a moving average value of the calculated absorbance. And an average value calculation unit 8c.

  The transmittance calculating unit 8a determines the transmittance according to the ratio between the intensity of each wavelength of the primary light emitted from the light source 3 and transmitted through the measurement blood BL and the intensity of each wavelength of the transmitted light transmitted through the measurement blood BL. Is calculated. For example, the intensity of the primary light is measured by receiving the primary light in a state where the blood collection tube 1 is not supported by the support base 2. As a configuration in which the intensity of the primary light is not measured in advance, the light emitted from the light source unit 6 is divided into two light beams by, for example, a beam splitter, one of which is used as the primary light, and the remaining light beam is measured blood. An apparatus configuration for irradiating the BL can be mentioned. When the intensity of the primary light is the same value over a predetermined wavelength range necessary for the measurement or the intensity spectrum is known, it is not necessary to measure the intensity of the primary light.

  The absorbance calculation unit 8b calculates the absorbance for each wavelength based on the common logarithm of the transmittance of each wavelength calculated by the transmittance calculation unit 8a.

  The average value calculation unit 8c calculates a moving average value by moving average using the nearest predetermined number of absorbances calculated for each wavelength. The moving average in this embodiment is a simple moving average.

  The coagulation detection unit 9 is predetermined from a differential operation unit 9a that secondarily differentiates the moving average value of absorbance with a wavelength, and a secondary differential absorbance that is based on the measured second derivative absorbance (hereinafter also referred to as a reference second derivative absorbance). A coagulation determination unit 9b that determines that the measured blood BL is coagulated when the distance is greater than or equal to the threshold value. The coagulation detection unit 9 displays the determination result of the coagulation determination unit 9b on the display 5d. The standard second derivative absorbance is an absorbance obtained by secondarily differentiating the moving average value of the absorbance of non-coagulated blood that has not been coagulated with respect to wavelength. This reference second derivative absorbance is calculated in advance prior to detection of coagulation, and is stored in a reference second derivative absorbance storage unit (hereinafter also referred to as a reference storage unit) 10 set in a predetermined area of the memory 5b.

  The differential calculation unit 9a secondarily differentiates the moving average value of the absorbance input from the average value calculation unit 8c by the wavelength, and calculates the measured secondary differential absorbance. The differentiation is performed based on a so-called finite difference method, and is performed by dividing the difference between the moving average values of two wavelengths separated by a minute wavelength by the minute difference in wavelength. Similarly, the second derivative is executed by dividing the difference between two wavelengths separated by a minute wavelength in the first derivative absorbance of the spectrum obtained by the first derivative by the minute difference in wavelength. This second derivative is performed on the measured first derivative absorbance in a predetermined wavelength range to obtain a measured second derivative absorbance spectrum (hereinafter also referred to as a measurement spectrum). Then, in the measurement spectrum, when a peak of absorbance exists in a specific wavelength range 11 and the measured second derivative absorbance is separated from the reference second derivative absorbance by a predetermined threshold or more, the coagulation of the measured blood BL is detected. To do. The specific wavelength region 11 is 750 to 1050 nm.

  In such a configuration, by executing the blood coagulation detection program, each unit of the information processing apparatus 6 described above functions. And the coagulation | solidification of the measurement blood BL accommodated in the blood collection tube 1 is detected by the procedure demonstrated below. In the blood coagulation detection program, the calculated transmittance, absorbance, moving average value, and second derivative absorbance are stored in the memory 5b as necessary, and are deleted when they are no longer needed.

  First, in a state where the blood collection tube 1 is not supported by the support base 2, the intensity of the primary light from the light source 3 is measured over a predetermined wavelength range. Measurement is performed on the intensity of each wavelength in a predetermined wavelength region. The measured intensity of each wavelength is used to calculate the transmittance that is the basis for calculating the absorbance.

  After measuring the intensity of the primary light from the light source 3, the blood collection tube 1 in which the collected measurement blood BL is accommodated is inserted into the recess 2 a of the support base 2 and accommodated in the blood collection tube 1 supported by the support base 2. The measured blood BL is irradiated with light.

  When the light receiver 5 receives the transmitted light that has passed through the measurement blood BL, the transmittance is calculated from the intensity of the transmitted light and the intensity of the primary light for each wavelength. Next, the absorbance for each wavelength is calculated by calculating the common logarithm of the transmittance based on the calculated transmittance. Thereafter, the moving average value of the absorbance is calculated from the predetermined number of calculated absorbances.

  A measured spectrum of the measured second derivative absorbance is obtained by secondarily differentiating the calculated moving average value by wavelength. In the measurement spectrum, when it is determined that a peak exists in a specific wavelength range 11 and thereby the measured secondary differential absorbance is more than a predetermined threshold from the reference secondary differential absorbance stored in the reference storage unit 11 In addition, it is detected that the measurement blood BL in the blood collection tube 1 is coagulated.

  Specifically, coagulation is detected by calculating a measured absorbance difference, which is the absolute value of the deviation between the measured second derivative absorbance and the reference second derivative absorbance for each wavelength, and the measured absorbance difference is equal to or greater than a predetermined threshold. In this case, it is determined by determining that the measurement blood BL is coagulated. As a specific example thereof, (1) it is determined that coagulation occurs when the measured absorbance difference at any wavelength in a specific wavelength region 11 is equal to or greater than a predetermined threshold. (2) Calculate the measured absorbance difference for all wavelengths within the specific wavelength range 11, calculate the average value of the measured absorbance difference based on the calculation result, and the average value of the measured absorbance difference is equal to or greater than a predetermined threshold value If so, solidification is determined. (3) In a specific wavelength range 11, when the number of times the measured second derivative absorbance is separated from the reference second derivative absorbance by a predetermined threshold or more is a predetermined number of times or more, coagulation is determined.

  FIG. 3 shows measured second derivative absorbance spectra of non-coagulated noncoagulated blood and measured second derivative absorbance spectra of coagulated blood coagulated in three different samples 1-3. It is the shown spectrum figure. In FIG. 3, in each spectrum diagram, the horizontal axis represents the wavelength, and the vertical axis represents the second derivative absorbance.

  In the spectrum diagram of FIG. 3, in common with samples 1 to 3, the measured second derivative absorbance spectrum of non-coagulated blood (indicated by a one-dot chain line in FIG. 3) is a specific wavelength region (indicated by a dotted ellipse in FIG. 3). ) There is no remarkable peak at 10 and it shows a stable state with almost no change. On the other hand, in the measurement spectrum of coagulated blood (shown by a solid line in the figure), there are significant upward and / or downward peaks in a specific wavelength region 11. In Samples 1 to 3 shown in FIG. 3, these peaks are confirmed when the wavelengths in the specific wavelength region 11 are 900 nm and 940 nm. These peaks do not appear in the spectrum of the original absorbance calculated based on the transmittance, but appear only when the original absorbance is second-order differentiated with respect to the wavelength.

  On the other hand, in a wavelength range other than the specific wavelength range 11, the measurement spectrum of non-coagulated blood and the measurement spectrum of coagulated blood show almost the same tendency. That is, in the range from around 700 nm to a specific wavelength region 11, each spectrum shows a stable second derivative absorbance with little change in the same peak or almost the same value. Moreover, in the wavelength range above the specific wavelength range 11, peaks appear almost the same. Therefore, it becomes difficult to detect the coagulation of the measurement blood BL in a wavelength range other than the specific wavelength range 11.

  Such a difference in the measurement spectrum is considered to be caused by the dispersion state of the components of the coagulated blood being different from that of the non-coagulated blood. That is, when the measurement blood BL is not coagulated, blood cell components such as red blood cells and white blood cells are dispersed throughout the measurement blood BL, so that the primary light from the light source 3 is reflected in the measurement blood BL and partly absorbed. Is done. On the other hand, when the measurement blood BL is coagulated, blood cell components and the like are aggregated to form a blood clot CL, and the distribution state of the blood cell components is different from that before coagulation in the portion of the serum SR formed at the same time. As a result, the component itself of the measurement blood BL does not change as a whole of the measurement blood BL, so that the primary light from the light source 3 is slightly reflected / diffused in the serum SR portion, but the light receiver 5 and the blood clot CL is generated, the primary light absorption in the clot CL increases. As a result, it is considered that the difference in the measurement spectrum as described above may occur between the coagulated blood with the clot CL and the non-coagulated blood without the clot CL.

  Therefore, as described above, the blood collection tube 1 is rotated in the vertical direction only by inserting the blood collection tube 1 containing the measurement blood BL into the support 2 and irradiating the measurement blood BL with light in a predetermined wavelength range. Since the coagulation of the measurement blood BL can be detected without changing the position, the time required for detection can be shortened as much as possible.

  In this embodiment, the moving average value of the original absorbance is second-order differentiated by the wavelength to calculate the measured second-derivative absorbance, and the coagulation detection is performed based on the measured second-derivative absorbance. Can be minimized. For this reason, the detection accuracy of coagulation can be increased. Instead of calculating the moving average value, noise removal may be performed by curve fitting, least squares, or the like.

  In addition, since the coagulation determination is performed based on the difference in absorbance in a specific wavelength region 11 including infrared rays, a label on which information on the measurement blood BL to be examined is written is attached to the surface of the blood collection tube 1. However, it is possible to detect the coagulation of the measurement blood BL with high accuracy.

  The present invention is not limited to the above embodiment.

  In the above embodiment, the transmitted light is split and received by the spectroscope 4, but the spectroscope 4 is disposed between the light source 3 and the light source side opening 2b, and the primary light emitted from the light source 3 is described. The light may be dispersed by the spectroscope 4 and the measurement blood BL may be irradiated with monochromatic light obtained by the spectroscopy. Even in this case, the absorbance is second-order differentiated for each wavelength in the predetermined wavelength region, and a measured second-derivative absorbance spectrum is created based on the measured second-derivative absorbance of each obtained wavelength. Coagulation of the measured blood BL is detected based on the second derivative absorbance peak.

  In the above embodiment, coagulation was detected based on the measured second derivative absorbance calculated by second derivative of the moving average value of the original absorbance, but the measured second derivative absorbance was calculated by secondarily differentiating the original absorbance with the wavelength. Then, the coagulation may be detected based on the calculated second derivative absorbance.

  In the embodiment described above, it is possible to determine the degree to which the measurement blood BL is coagulated by setting a plurality of predetermined threshold values. For example, the predetermined threshold is configured by a first threshold having a small value, a second threshold greater than the first threshold, and a third threshold greater than the second threshold. Then, when the measured second derivative absorbance is separated from the reference second derivative absorbance by a first threshold or more, it is determined that coagulation has started. Similarly, when the measured second derivative absorbance is separated from the reference second derivative absorbance by a second threshold or more, it is determined that the coagulation has progressed to an intermediate level. Thereafter, when the measured second derivative absorbance is separated from the reference second derivative absorbance by a third threshold or more, it is determined that the coagulation is complete. Thus, the degree of coagulation can be detected by determining the change in the measured second derivative absorbance spectrum with respect to the passage of time from the start of the coagulation detection operation based on the first to third threshold values.

  In the above embodiment, the specific wavelength range 11 is set at a wavelength of 750 to 1050 nm. However, the specific wavelength range may be set to a wavelength of 500 nm to 650 nm. Even in the wavelength range of 500 nm to 650 nm, as is apparent from FIG. 3, the difference in absorbance between the standard second derivative absorbance and the measured second derivative absorbance is recognized. Can be detected.

  In the above embodiment, the light source 3 and the light receiver 5 are arranged in the same straight line at the position where the blood collection tube 1 is sandwiched, but they may be arranged as shown in FIGS. That is, what is shown in FIGS. 4 to 6 is a modification in which two light sources 3 are arranged for one light receiver 5. In the first modification shown in FIG. 4, each light source 3 is disposed on the opposite side of the light receiver 5 with the blood collection tube 1 interposed therebetween. In the second modification shown in FIG. 5, the light source 3 is disposed on the same side as the light receiver 5 with respect to the blood collection tube 1 and above and below the light receiver 5. The third modified example shown in FIG. 6 is a configuration in which the configuration of the light source 3 and the light receiver 5 in the first modified example is adopted at a position sandwiching the blood collection tube 1. In these configurations, the light source 3 and the light receiver 5 are arranged in the same vertical plane.

  Next, in the fourth modification shown in FIG. 7, the light source 3 and the light receiver 5 are arranged in the same horizontal plane, and the light source 3 is rotated by 90 degrees in the same plane with respect to the light receiver 5. Is to be placed. In the fifth modification shown in FIG. 8, the light source 3 is further increased by one with respect to the fourth modification, and the light sources 3 are arranged to face each other.

  In the arrangement structure of the light source 3 and the light receiver 5 shown in the first to fifth modified examples, the reflected / scattered light generated in the coagulation portion of the measurement blood BL in addition to the transmitted light (broken arrows in FIGS. 4 to 8) Can be efficiently received, so that the analysis accuracy can be further improved.

  In addition, it goes without saying that the present invention is not limited to the above-described embodiment, and various modifications can be made without departing from the spirit of the present invention.

1 ... Blood collection tube (container)
2... Support stand 3... Light source 5... Receiver 6. Information processing device 8... Absorbance calculation unit 8 a. 8b ··· Absorbance calculation unit 8c ··· Average value calculation unit 9 ··· Coagulation detection unit 9a · · · Differential operation unit 10 · · · Specific wavelength range 100 · · · Blood coagulation detection Device BL ... Measurement blood

Claims (6)

A light source for irradiating the measurement blood contained in the container with light;
A light receiver that receives the transmitted light that has passed through the measurement blood;
An absorbance calculation unit that calculates the absorbance over a predetermined wavelength range based on the received transmitted light; and
A blood coagulation detection apparatus comprising: a coagulation detection unit that secondarily differentiates the absorbance with a wavelength and detects coagulation of the measured blood based on a measured second derivative absorbance that is a second derivative result thereof. The absorbance calculation unit includes a transmittance calculation unit that calculates the transmittance of the transmitted light, and an absorbance calculation unit that calculates the absorbance based on the calculated transmittance.
The coagulation detector includes a differential operation unit that secondarily differentiates the absorbance with a wavelength, and a second derivative absorbance that is based on the measured second derivative absorbance (hereinafter referred to as a reference second derivative absorbance) that is a predetermined threshold value or more. The blood coagulation detection apparatus according to claim 1, further comprising a coagulation determination unit that determines that the measurement blood is coagulated when separated. The absorbance calculation unit further comprises an average value calculation unit for calculating a moving average value for the wavelength of the absorbance,
The blood coagulation detection apparatus according to claim 2, wherein the differential operation unit is a unit that secondarily differentiates the moving average value with a wavelength.   4. The blood coagulation according to claim 1, 2, or 3, wherein the coagulation detector detects blood coagulation when the measured second derivative absorbance is separated from a reference second derivative absorbance by a predetermined threshold or more at a wavelength of 750 to 1050 nm. Detection device. An irradiation step for irradiating the measurement blood contained in the container with light;
A light receiving step for receiving the transmitted light that has passed through the measurement blood;
An absorbance calculation step for calculating an absorbance over a predetermined wavelength range based on the received transmitted light;
A blood coagulation detection method, comprising: a coagulation detection step of secondarily differentiating the absorbance with a wavelength and detecting coagulation of the measured blood in the container based on the measured second derivative absorbance which is the second derivative result thereof. .   Based on the transmitted light that has passed through the collected measurement blood, an absorbance calculation unit that calculates the absorbance over a predetermined wavelength range, and the absorbance is second-order differentiated by wavelength, and the second-order derivative measurement result is the second-order derivative absorbance. A blood coagulation detection program which causes a computer to function as a coagulation detection unit for detecting coagulation of measured blood in a container based on the computer program.