JP2016028606A5

JP2016028606A5 -

Info

Publication number: JP2016028606A5
Application number: JP2015224220A
Authority: JP
Filing date: 2015-11-16
Publication date: 2016-04-14
Anticipated expiration: 2035-03-31

Claims

An acquisition means for acquiring captured image data as captured image data including a culture medium image in which a culture medium in which a specimen is placed and a colony is cultured is imaged;
Scanning on the captured image based on a template image for specifying the shape of the culture medium, and specifying an area having a predetermined condition as an image portion of the culture medium image, from among the captured images Image extracting means for extracting an image portion of the medium image;
Detecting means for performing image analysis on the extracted medium image, identifying a colony pixel used for imaging the colony cultured in the medium, and detecting the colony;
With
The template image includes a first region having the same shape as the medium to be extracted ;
Colonies detecting said first region, characterized in that it is a mask area that have the property to disable the color feature amount of each pixel of the captured image if the first area on the captured image is superimposed system.

In the colony detection system according to claim 1,
The template image further includes a second region having a predetermined shape on the periphery of the first region ,
The second region is a region having characteristics that enable the color feature amount of each pixel of the captured image when the first region is superimposed on the captured image ,
The image extracting means;
While masking the region superimposed on the first region at each scanning position, based on the color feature amount of each pixel belonging to the region superimposed on the second region, the medium image is selected from the captured images. A colony detection system characterized by extracting an image portion .

In the colony detection system according to claim 2,
The template image includes a third region formed at an outer edge of the second region ;
The colony detection system, wherein the third region is a mask region having a characteristic of invalidating a color feature amount of each pixel of the captured image when the third region is superimposed on the captured image.

In the colony detection system of any one of Claims 1-3,
The acquisition means acquires type information indicating the type of the medium,
The colony detection system, wherein the image extraction unit selects one template image from the plurality of template images based on the acquired type information, and extracts the culture medium image using the selected template image .

In the colony detection system according to claim 4,
The image extracting means determines a search area where the medium image is assumed to be formed from the captured image based on the type information, and scans the selected template image within the determined search area. A colony detection system that extracts the medium image by

In the colony detection system of any one of Claims 1-3,
The acquisition means acquires type information indicating the type of the medium,
The image extraction means determines a search area where the culture medium image is assumed to be formed from the captured image based on the type information, and scans the template image within the determined search area. Colony detection system that extracts media images.

In the colony detection system of any one of Claims 1-6,
The detection means is
Feature quantity extraction means for extracting color feature quantities relating to the colors of pixels constituting the extracted medium image;
Identifying means for identifying a colony pixel used to image the colony based on the color feature of each extracted pixel;
Based on the identified colony pixels, colony detection means for detecting the colonies cultured in the medium;
A colony detection system comprising:

In the colony detection system according to claim 7,
The acquisition means acquires type information indicating the type of the medium,
The colony detection system in which the specifying unit specifies the colony pixel based on the acquired type information together with the extracted color feature amount of each pixel.

In the colony detection system according to claim 7 or 8,
The acquisition means acquires type information indicating the type of the medium,
The colony detection system in which the colony detection unit detects the colonies cultured in the medium based on the acquired type information together with the identified colony pixels.

In the colony detection system of any one of Claims 1-9,
A colony detection system further comprising registration means for registering information on the detected colonies as culture medium information in a database.

Computer
An acquisition means for acquiring captured image data as captured image data including a culture medium image in which a culture medium in which a specimen is placed and a colony is cultured is imaged;
Scanning on the captured image based on a template image for specifying the shape of the culture medium, and specifying an area having a predetermined condition as an image portion of the culture medium image, from among the captured images Image extracting means for extracting an image portion of the medium image, and performing image analysis on the extracted medium image to identify a colony pixel used for imaging the colonies cultured in the medium Detection means for detecting the colony;
The template image includes a first region having the same shape as the medium to be extracted ,
The program first region, characterized in that it is a mask area that have the characteristics to disable the color feature amount of each pixel of the captured image if the first area on the captured image is superimposed.

The program according to claim 11,
The template image further includes a second region having a predetermined shape on the periphery of the first region ,
The second region is a region having characteristics that enable the color feature amount of each pixel of the captured image when the first region is superimposed on the captured image ,
The image extracting means;
While masking the region superimposed on the first region at each scanning position, based on the color feature amount of each pixel belonging to the region superimposed on the second region, the medium image is selected from the captured images. A program that extracts image parts .

The program according to claim 12,
The template image includes a third region formed at an outer edge of the second region ;
The program, wherein the third region is a region having a characteristic of invalidating a color feature amount of each pixel of the captured image when the third region is superimposed on the captured image.

An acquisition step of acquiring, as captured image data, data of a captured image including a culture medium image in which a culture medium in which a specimen is placed and a colony is cultured is imaged;
Scanning on the captured image based on a template image for specifying the shape of the culture medium, and specifying an area having a predetermined condition as an image portion of the culture medium image, from among the captured images An image extraction step of extracting an image portion of the medium image;
A detection step of performing image analysis on the extracted medium image, identifying a colony pixel used for imaging the colony cultured in the medium, and detecting the colony;
Including
The template image includes a first region having the same shape as the medium to be extracted ;
The colony detection method, wherein the first region is a mask region having a characteristic of invalidating a color feature amount of each pixel of the captured image when the first region is superimposed on the captured image. .

In the colony detection method according to claim 14,
The template image is
Said template image, further to include a second region having a predetermined shape on the periphery of the first region,
The second region is a region having characteristics that enable the color feature amount of each pixel of the captured image when the first region is superimposed on the captured image ,
The image extracting means;
While masking the region superimposed on the first region at each scanning position, based on the color feature amount of each pixel belonging to the region superimposed on the second region, the medium image is selected from the captured images. A colony detection method for extracting an image portion .

In the colony detection method of Claim 15,
The template image includes a third region formed at an outer edge of the second region ;
The colony detection method, wherein the third region is a region having a characteristic of invalidating a color feature amount of each pixel of the captured image when the third region is superimposed on the captured image.

In the colony detection system according to claim 5 or 6,
When the image extraction processing unit scans the template image within the determined search area, each scan position where the template image is superimposed on the captured image, and a search area adjacent to each scan position The medium is scanned by scanning the template image while limiting the scanning range in the search region based on the relationship between the scanning position and the color feature amount of the predetermined region on the captured image specified by the template image. Colony detection system that extracts images.

The colony detection system according to claim 17,
The image extraction processing means, each scanning position where the template image in horizontal scanning or vertical scanning is superimposed on the captured image, each scanning position where the template image is superimposed on the captured image based on the immediately preceding scanning position, The colony detection system which restrict | limits the said search area | region based on the relationship of the color feature-value of the predetermined area | region on the captured image specified by the said template image in.

The colony detection system according to claim 17,
The image extraction processing unit is on a captured image specified by the template image at a specific scanning position where the template image is superimposed on the captured image and a plurality of scanning positions adjacent to the specific scanning position. The colony detection system which restrict | limits the said search area | region based on the relationship of the color feature-value of a predetermined area | region.

The colony detection system according to claim 17,
The image extraction processing means includes, in any single horizontal scanning line or vertical scanning, each scanning position where the template image is superimposed on the captured image, and a scanning position on a search area adjacent to each scanning position. The range of the search area for performing horizontal scanning or vertical scanning is specified based on the relationship between the color feature amounts of the predetermined area on the captured image specified by the template image in, and the horizontal scanning is performed in the specified range. Or the colony detection system which extracts the said culture medium image by scanning the said template image, restrict | limiting vertical scanning.