JP2015231365A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2015231365A5 JP2015231365A5 JP2015098423A JP2015098423A JP2015231365A5 JP 2015231365 A5 JP2015231365 A5 JP 2015231365A5 JP 2015098423 A JP2015098423 A JP 2015098423A JP 2015098423 A JP2015098423 A JP 2015098423A JP 2015231365 A5 JP2015231365 A5 JP 2015231365A5
- Authority
- JP
- Japan
- Prior art keywords
- cells
- culturing
- stem cells
- inner ear
- pluripotent stem
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000004027 cells Anatomy 0.000 claims 20
- 230000001939 inductive effect Effects 0.000 claims 11
- 210000001778 pluripotent stem cell Anatomy 0.000 claims 9
- 210000003027 Ear, Inner Anatomy 0.000 claims 8
- 210000000130 stem cell Anatomy 0.000 claims 7
- 102100008634 FGF2 Human genes 0.000 claims 6
- 101700082364 FGF2 Proteins 0.000 claims 6
- 230000002792 vascular Effects 0.000 claims 5
- 102100000261 FGF10 Human genes 0.000 claims 2
- 101700052889 FGF10 Proteins 0.000 claims 2
- 102100008645 FGF3 Human genes 0.000 claims 2
- 101710004181 INTS2 Proteins 0.000 claims 2
- 102000011384 Pendrin Human genes 0.000 claims 2
- 108050001616 Pendrin Proteins 0.000 claims 2
- 239000000835 fiber Substances 0.000 claims 2
- 239000003102 growth factor Substances 0.000 claims 2
- 239000003112 inhibitor Substances 0.000 claims 2
- 230000002401 inhibitory effect Effects 0.000 claims 2
- 239000011435 rock Substances 0.000 claims 2
- 238000004114 suspension culture Methods 0.000 claims 2
- 210000001671 Embryonic Stem Cells Anatomy 0.000 claims 1
- 210000000981 Epithelium Anatomy 0.000 claims 1
- 102100015614 FGF19 Human genes 0.000 claims 1
- 101700047578 FGF19 Proteins 0.000 claims 1
- 102100007142 FGF20 Human genes 0.000 claims 1
- 101700028871 FGF20 Proteins 0.000 claims 1
- 102100015612 FGF9 Human genes 0.000 claims 1
- 101700084870 FGF9 Proteins 0.000 claims 1
- 208000003098 Ganglion Cysts Diseases 0.000 claims 1
- UIIMBOGNXHQVGW-UHFFFAOYSA-M NaHCO3 Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims 1
- 208000005400 Synovial Cyst Diseases 0.000 claims 1
- 210000000270 basal cell Anatomy 0.000 claims 1
- 210000002768 hair cell Anatomy 0.000 claims 1
- 210000004255 neuroglia Anatomy 0.000 claims 1
- 210000002569 neurons Anatomy 0.000 claims 1
- 230000001953 sensory Effects 0.000 claims 1
Claims (9)
前記多能性幹細胞をROCK Inhibitor存在下で培養する工程と、
増殖因子非存在下、ROCK Inhibitor非存在下で培養する工程と、
無血清培地で培養する工程と、
bFGF, FGF3, FGF10, FGF19からなる群から選択される少なくとも一つの増殖因子を含有する無血清培地で培養する工程と、
単一細胞に解離する工程と
を、この順で行う、誘導方法。 A method for inducing pluripotent stem cells into inner ear stem cells,
Culturing the pluripotent stem cells in the presence of ROCK Inhibitor;
Culturing in the absence of growth factors and in the absence of ROCK Inhibitor;
Culturing in a serum-free medium;
culturing in a serum-free medium containing at least one growth factor selected from the group consisting of bFGF, FGF3, FGF10, and FGF19 ;
A method of induction wherein the step of dissociating into single cells is performed in this order.
をさらに含む、請求項１に記載の誘導方法。 Culturing the cells dissociated into single cells in a culture dish coated with poly-O-fibronectine;
The induction method according to claim 1 , further comprising:
請求項１の方法によって、内耳幹細胞を製造する工程と、
前記内耳幹細胞をFGF9およびFGF20存在下で浮遊培養する工程と、
接着培養する工程と、
を、この順で行う、誘導方法。 A method for inducing inner ear sensory epithelium (support cells, hair cells) and cochlear ganglion cells (neurons, glial cells) from pluripotent stem cells ,
Producing inner ear stem cells by the method of claim 1;
A step of suspension culture of the inner ear stem cells in the presence of FGF9 and FGF20;
An adhesion culture step;
Are guided in this order.
請求項１の方法によって、内耳幹細胞を製造する工程と、
前記内耳幹細胞をFGF3および／またはFGF10存在下で浮遊培養する工程と、
接着培養する工程と、
を、この順で行う、誘導方法。 A method for inducing vascular marginal cells from pluripotent stem cells ,
Producing inner ear stem cells by the method of claim 1;
The suspension culture of the inner ear stem cells in the presence of FGF3 and / or FGF10;
An adhesion culture step;
Are guided in this order.
請求項１の方法によって、内耳幹細胞を製造する工程と、
前記内耳幹細胞をbFGF存在下で培養する工程を含む、誘導方法。 A method for inducing Periotic mesenchymal cells from pluripotent stem cells ,
Producing inner ear stem cells by the method of claim 1;
An induction method comprising a step of culturing the inner ear stem cells in the presence of bFGF.
請求項６の方法によって、Periotic mesenchymal 細胞を製造する工程と、
前記Periotic mesenchymal 細胞をbFGF存在下で培養する工程と、
bFGF非存在下で培養し、蝸牛線維細胞及び血管条細胞を得る工程と、
をこの順で行う、誘導方法。 A method for inducing cochlear fiber cells and vascular streak cells from pluripotent stem cells ,
Producing periotic mesenchymal cells by the method of claim 6;
Culturing the Periotic mesenchymal cells in the presence of bFGF;
culturing in the absence of bFGF to obtain cochlear fiber cells and vascular streak cells;
In this order, the guidance method.
請求項６の方法によって、Periotic mesenchymal 細胞を製造する工程と、
前記Periotic mesenchymal 細胞をbFGF存在下で培養する工程と、
bFGF非存在下で、NaHCO3存在下で培養し、Pendrin陽性細胞を得る工程と、
をこの順で行う。誘導方法。 A method for inducing Pendrin positive cells from pluripotent stem cells ,
Producing periotic mesenchymal cells by the method of claim 6;
Culturing the Periotic mesenchymal cells in the presence of bFGF;
culturing in the presence of NaHCO 3 in the absence of bFGF to obtain Pendrin positive cells;
In this order. Guidance method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2015098423A JP6218152B2 (en) | 2014-05-13 | 2015-05-13 | Inner ear cell induction method |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2014100017 | 2014-05-13 | ||
| JP2014100017 | 2014-05-13 | ||
| JP2015098423A JP6218152B2 (en) | 2014-05-13 | 2015-05-13 | Inner ear cell induction method |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015231365A JP2015231365A (en) | 2015-12-24 |
| JP2015231365A5 true JP2015231365A5 (en) | 2017-06-01 |
| JP6218152B2 JP6218152B2 (en) | 2017-10-25 |
Family
ID=54933292
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015098423A Active JP6218152B2 (en) | 2014-05-13 | 2015-05-13 | Inner ear cell induction method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP6218152B2 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6850025B2 (en) * | 2016-02-22 | 2021-03-31 | 学校法人順天堂 | Inner ear cell manufacturing method |
| JP2023106640A (en) * | 2020-06-09 | 2023-08-02 | 株式会社オトリンク | Method for producing inner ear hair cells, method for assessing drug, and composition for inducing differentiation of cells |
| JP2023106639A (en) * | 2020-06-09 | 2023-08-02 | 株式会社オトリンク | Method for producing inner ear progenitor cells, method for producing inner ear hair cells, method for assessing drug, and composition for inducing differentiation of inner ear cells |
| JPWO2023033149A1 (en) | 2021-09-03 | 2023-03-09 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2590036T3 (en) * | 2009-08-12 | 2016-11-17 | Kyoto University | Method for inducing differentiation of pluripotent stem cells into neural precursor cells |
| WO2013166488A1 (en) * | 2012-05-04 | 2013-11-07 | Indiana University Research & Technology Corporation | Methods for generating the inner ear and other cranial placode-derived tissues using pluripotent stem cells |
| WO2013187416A1 (en) * | 2012-06-12 | 2013-12-19 | 学校法人 慶應義塾 | METHOD FOR AMPLIFYING iPS CELL SUITABLE FOR NEURAL DIFFERENTIATION, AND METHOD FOR INDUCING NEURAL STEM CELL |
-
2015
- 2015-05-13 JP JP2015098423A patent/JP6218152B2/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2015231365A5 (en) | ||
| Singh et al. | Up-scaling single cell-inoculated suspension culture of human embryonic stem cells | |
| IL307568A (en) | Photoreceptors and photoreceptor progenitors produced from pluripotent stem cells | |
| PH12016502274A1 (en) | Use of small molecules to enhance mafa expression in pancreatic endocrine cells | |
| WO2015054526A3 (en) | Methods of mammalian retinal stem cell production and applications | |
| EP4345160A3 (en) | Method for reproducible differentiation of clinical-grade retinal pigment epithelium cells | |
| RU2009115186A (en) | CULTIVATION ENVIRONMENT FOR STEM CELLS AND METHOD | |
| CA2821065C (en) | Culture method to obtain and maintain a pure or enriched population of mammalian neural stem cells and/or neural progenitor cells that are prone to differentiate into oligodendrocyte-lineage cells in vitro | |
| HRP20201917T1 (en) | Method for producing otic progenitors | |
| Villegas et al. | FGF signalling as a mediator of lineage transitions—evidence from embryonic stem cell differentiation | |
| AU2017255099A1 (en) | Purification method for pancreatic precursor cells derived from pluripotent stem cells and amplification method therefor | |
| JP2020040996A5 (en) | ||
| MX2019005476A (en) | Pluripotent stem cell assay. | |
| JP2016504014A5 (en) | ||
| JP2015213495A5 (en) | ||
| WO2014087244A3 (en) | Methods of producing rpe cells | |
| Wu et al. | Increased culture density is linked to decelerated proliferation, prolonged G1 phase, and enhanced propensity for differentiation of self-renewing human pluripotent stem cells | |
| WO2019241684A8 (en) | Synthetic 5' utr sequences, and high-throughput engineering and screening thereof | |
| EP4004191A4 (en) | Precursor cells of induced pluripotent stem cell-derived mesenchymal stem cells and method for preparing the same | |
| EA202091354A1 (en) | METHOD FOR CULTIVATION OF CELLS | |
| JP6218152B2 (en) | Inner ear cell induction method | |
| CN105121633A (en) | Medium for establishing neuroepithelial stem cells and method and use thereof | |
| WO2015111075A3 (en) | Method of increasing biomass and lipid content in a micro-organism and a genetically modified micro-organism exhibiting enhanced autophagy | |
| IL289452A (en) | Method for producing culture medium composition for suspension culturing adherent cells | |
| MY152746A (en) | Altering enzyme balance through fermentation conditions |