JP2015224327A - Detergent composition for textile product - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a detergent composition for textile products which has excellent detergency against mud dirt of textile products and a detergent composition for textile products which has excellent re-soiling preventive performance against mud dirt of textile products.SOLUTION: A detergent composition for textile products contains a transferrin family protein. A method of cleaning mud dirt of textile products using the detergent composition for textile products and a method of preventing re-soiling of textile products with mud dirt using the detergent composition for textile products are also provided.

Description

  The present invention relates to a detergent composition for textile products.

Dirt adhering to textiles such as clothing is generally classified into organic dirt and inorganic dirt. Among these, a typical example of inorganic dirt is mud dirt. Mud dirt adhering to textiles, especially mud dirt adhering to clothing such as socks, is frequently generated. And since the mud dirt adhering to a textile product adheres mud particles firmly to a fiber, it has been regarded as one of the dirts that are difficult to remove from the past.
The cleaning effect of surfactants and builder mud stains, which are the main components of textile cleaning agents, is relatively small compared to organic stains. Therefore, various techniques have been developed so far in order to improve the cleaning effect of the cleaning agent on the inorganic soil.

So far, there have been proposed cleaning compositions containing various enzymes as a base material that exhibits a certain effect on mud dirt adhering to textiles such as clothing.
For example, Patent Document 1 describes a detergent composition containing protopectinase. Here, protopectinase is an enzyme that catalyzes the reaction of insoluble pectin to soluble pectin. In the invention described in Patent Document 1, the pectin of the fiber to which mud dirt adheres is peeled off and the mud dirt is washed.
Patent Documents 2 to 5 describe cleaning compositions containing cellulase. In the inventions described in Patent Documents 2 to 5, the part of the fiber is decomposed by the action of cellulase or the structure of the cellulose constituting the fiber is changed to release the mud adhering to the fiber, and the mud dirt is removed. ing.
Furthermore, Patent Document 6 describes a detergent composition containing a specific enzyme (lipase, protease, cellulase, amylase, etc.) having an affinity for mud. The invention described in Patent Document 6 is a cleaning composition for mud composite soil in which fat, protein, and the like are combined with mud. In the invention described in Patent Document 6, the enzyme acts selectively on mud, decomposes or removes lipids and proteins forming a mud-soil complex, and includes surfactants contained in the detergent composition. The mud soil present in the vicinity of the enzyme is removed by the metal scavenger.

Japanese Patent Application Laid-Open No. 11-1404089 JP-A-61-47800 JP-A-61-231094 JP 2000-319688 A JP-T-2006-517989 JP 2004-210812 A

This invention makes it a subject to provide the cleaning composition for textiles which has the outstanding cleaning property with respect to the mud dirt of textiles.
Moreover, this invention makes it a subject to provide the cleaning composition for textiles which has the re-contamination prevention ability excellent with respect to the mud dirt of textiles.

  In view of the above problems, the present inventors have intensively studied. As a result, it has been found that transferrin family proteins such as lactoferrin, serotransferrin and ovotransferrin have excellent detergency and anti-recontamination ability against mud soil of textile products. The present invention has been completed based on this finding.

That is, this invention relates to the cleaning composition for textiles containing a transferrin family protein.
The present invention also relates to a method for cleaning mud soil adhering to a textile product, using the detergent composition for textile products.
Furthermore, this invention relates to the method of preventing the re-contamination of the textiles by a mud stain using the said cleaning composition for textiles.

The cleaning composition for textiles of the present invention has excellent cleaning properties against mud stains on textiles.
Moreover, the cleaning composition for textiles of the present invention has an excellent ability to prevent recontamination against mud stains on textiles.

It is a figure which shows the result of having aligned by ClustalW (DDBJ) and comparing the amino acid sequence about the amino acid sequence shown to sequence number 1-3.

The cleaning composition for textiles of the present invention (hereinafter also simply referred to as “cleaning composition”) contains a transferrin family protein as a cleaning component for mud soil of textiles.
As shown in the examples described later, transferrin family proteins are used for sanitary goods (towels, bedding, etc.), fabric products (curtains, carpets, etc.), textile products such as clothing (hereinafter referred to simply as “fiber products” in the present specification). It also has excellent detergency and recontamination prevention capability against mud stains. Therefore, the detergent composition for textiles of the present invention containing the transferrin family protein can be suitably used as a detergent for textiles.
In this specification, “preventing recontamination of textile products due to mud stains” means that mud stain substances dispersed in an aqueous solution prevent reattachment to textiles such as cloth present in the aqueous solution. To do.

In the present specification, the “transferrin family protein” is a glycoprotein having iron binding ability and is a general term for proteins responsible for transport of iron ions in vivo. Transferrin family proteins have a structure in which two lobes called N-lobe and C-lobe are linked by one linker. One iron ion can bind to each of the two lobes. In the present specification, “glycoprotein having iron-binding ability” means a glycoprotein in which each of the two lobes can bind to one iron ion.
Although there are some unclear points regarding the mechanism by which transferrin family proteins have a detergency and anti-recontamination ability against mud soil, they have enzyme activities such as pectinase activity, endoglucanase activity, lipase activity, protease activity, and amylase activity. This is the first finding found that no transferrin family proteins have the ability to clean and prevent re-contamination against mud soil.

  Transferrin family proteins that can be used in the present invention are described in Lambert et al., Comparative Biochemistry and Physiology, Part B, 142 (2005), p. 129-141; Testa, Proteins of iron metabolism, CRC Press, 2002; and Harris & Aisen, Iron carriers and iron proteins, Vol. 5, descriptions of Physical Bioinorganic Chemistry, VCH, 1991, etc. can be referred to.

  Specific examples of transferrin family proteins include lactoferrin, serotransferrin, ovotransferrin, melanotransferrin and the like. Of these, lactoferrin, serotransferrin, and ovotransferrin are preferred.

  Lactoferrin is also called lactotransferrin and lactsiderophilin, and is a glycoprotein having iron-binding ability contained in exocrine fluids such as mammalian milk, tears, sweat, and saliva. In addition, the lactoferrin in this specification also includes apolactoferrin obtained by removing iron from lactoferrin, and metal saturated or partially saturated lactoferrin obtained by chelating apolactoferrin with a metal such as iron, copper, zinc, or manganese.

In the present invention, the lactoferrin is preferably bovine derived lactoferrin, and more preferably bovine milk derived lactoferrin. Here, one example of an amino acid sequence of a protein contained in bovine-derived lactoferrin is shown in SEQ ID NO: 1.
The lactoferrin used in the present invention is a glycoprotein containing a protein consisting of the amino acid sequence shown in SEQ ID NO: 1, or one or several (preferably 2-212, more preferably 2-142, and still more preferably) in the amino acid sequence. 2 to 71, more preferably 2 to 35, particularly preferably 2 to 7) glycoproteins having iron-binding ability, including proteins comprising amino acid sequences in which amino acids have been deleted, substituted, added or inserted. Preferably there is.
Alternatively, it consists of an amino acid sequence having a homology with the amino acid sequence shown in SEQ ID NO: 1 of 70% or more (preferably 80% or more, more preferably 90% or more, even more preferably 95% or more, particularly preferably 99% or more). Glycoproteins containing proteins and having iron binding ability are preferred. Here, the homology of amino acid sequences refers to the ratio (%) of the number of positions where identical amino acid residues exist in both sequences when the two amino acid sequences are aligned to the total number of amino acid residues. Specifically, it is calculated by the Lippman-Pearson method (Lipman-Pearson method; Science, 227, 1435, (1985)), and homology analysis of genetic information processing software Genetyx-Win (Ver. 5.1.1; software development) ( It can be calculated by performing an analysis with a unit size to compare (ktup) of 2 using a Search homology) program.
In addition, the sugar chain part of lactoferrin can be appropriately selected within a range in which the effects of the present invention are exhibited.

Serotransferrin is also referred to as didelophilin and transferritin, and is a glycoprotein having an iron binding ability contained in plasma. In the present invention, the serotransferrin is preferably human-derived serotransferrin, and more preferably human blood-derived serotransferrin. Here, an example of the amino acid sequence of a protein contained in human-derived cellotransferrin is shown in SEQ ID NO: 2.
The cellotransferrin used in the present invention is a glycoprotein containing a protein consisting of the amino acid sequence shown in SEQ ID NO: 2, or one or several (preferably 2 to 209, more preferably 2 to 140, more preferably) in the amino acid sequence. 2 to 70, more preferably 2 to 35, particularly preferably 2 to 7) glycoproteins having an iron-binding ability, including a protein having an amino acid sequence in which amino acids are deleted, substituted, added or inserted It is preferable that
Alternatively, it consists of an amino acid sequence having a homology with the amino acid sequence shown in SEQ ID NO: 2 of 70% or more (preferably 80% or more, more preferably 90% or more, further preferably 95% or more, particularly preferably 99% or more). Glycoproteins containing proteins and having iron binding ability are preferred.
The sugar chain portion of cellotransferrin can be appropriately selected within the range where the effects of the present invention are exhibited.

Ovotransferrin is also called conalbumin and is a glycoprotein having iron binding ability contained in egg white. In the present invention, ovotransferrin is preferably ovotransferrin derived from chicken egg white. Here, an example of the amino acid sequence of the protein contained in egg white-derived ovotransferrin is shown in SEQ ID NO: 3.
The ovotransferrin used in the present invention is a glycoprotein containing a protein consisting of the amino acid sequence shown in SEQ ID NO: 3, or one or several (preferably 2-212, more preferably 2-141, still more preferably) in the amino acid sequence. 2 to 71, more preferably 2 to 35, particularly preferably 2 to 7) glycoprotein having an iron-binding ability, comprising a protein having an amino acid sequence deleted, substituted, added or inserted. It is preferable that
Alternatively, it consists of an amino acid sequence having a homology with the amino acid sequence shown in SEQ ID NO: 3 of 70% or more (preferably 80% or more, more preferably 90% or more, further preferably 95% or more, particularly preferably 99% or more). Glycoproteins containing proteins and having iron binding ability are preferred.
In addition, the sugar chain part of ovotransferrin can be appropriately selected within a range in which the effect of the present invention is exhibited.

About the amino acid sequence shown to sequence number 1-3, alignment was performed by ClustalW (DDBJ), and the amino acid sequence was compared. The result is shown in FIG.
When the above results were examined, when the amino acid sequence was aligned based on the amino acid sequence of SEQ ID NO: 1, the amino acid at the position corresponding to (x) in Table 1 shown below in the amino acid sequence shown in SEQ ID NO: 1 to 3 The residues are the amino acid residues shown in (y) of Table 1, and these amino acid residues were common among the three transferrin family proteins. From this result, it is considered that these common amino acid residues are involved in the detergency of transferrin family proteins used in the present invention against mud soil. Accordingly, when the transferrin family proteins used in the present invention are aligned with respect to the amino acid sequence of SEQ ID NO: 1, the amino acid residues corresponding to the position (x) in Table 1 shown below are shown in Table 1 below. The amino acid residue shown in (y) is preferred.

  The transferrin family protein used in the present invention may be a commercially available transferrin family protein, a transferrin family protein separated from milk, tears, sweat, saliva, plasma, egg white, etc. by a conventional method, or a gene. It may be a recombinant protein obtained by an engineering technique. In addition, the transferrin family protein in the present specification includes a fusion or conjugate in which a transferrin family protein and another protein or molecule are fused or conjugated.

  One type of transferrin family protein contained in the detergent composition of the present invention may be used, or two or more types may be used in combination.

  The content of the transferrin family protein in the detergent composition of the present invention can be appropriately set by those skilled in the art. For example, the content of the transferrin family protein is preferably 0.005% by mass or more, more preferably 0.05% by mass or more, and preferably 0.4% by mass or less in the total amount of the detergent composition containing the transferrin family protein, 0.2 mass% or less is more preferable. Or 0.005-0.4 mass% is preferable and 0.05-0.2 mass% is more preferable.

  In the cleaning composition of the present invention, any component usually used for this type of cleaning agent can be blended. Examples of such components include those shown below.

(1) Surfactant A surfactant can be blended in the cleaning composition of the present invention. In this invention, arbitrary surfactants, such as anionic surfactant, a nonionic surfactant, an amphoteric surfactant, and a cationic surfactant, are used individually by 1 type or in combination of 2 or more types. be able to. The surfactant that can be used in the present invention is preferably an anionic surfactant or a nonionic surfactant.

  Examples of the anionic surfactant include a sulfate ester salt of an alcohol having 10 to 18 carbon atoms, a sulfate ester salt of an alkoxylate of an alcohol having 8 to 20 carbon atoms, an alkylbenzene sulfonate, an alkyl sulfate ester salt, and a paraffin sulfonate. , Α-olefin sulfonate, α-sulfo fatty acid salt, α-sulfo fatty acid alkyl ester salt or fatty acid salt are preferred. In the present invention, linear alkylbenzene sulfonates and alkyl sulfates having an alkyl chain having 10 to 14 carbon atoms (more preferably 12 to 14 carbon atoms) are particularly preferred. As a counter ion of these salts, alkali metal salts and amines are preferable, and sodium, potassium, monoethanolamine, and diethanolamine are particularly preferable.

  Nonionic surfactants include polyoxyalkylene alkyl (carbon number 8-20) ether, alkyl polyglycoside, polyoxyalkylene alkyl (carbon number 8-20) phenyl ether, polyoxyalkylene sorbitan fatty acid (carbon number 8-8). 22) Ester, polyoxyalkylene glycol fatty acid (carbon number 8 to 22) ester, and polyoxyethylene polyoxypropylene block polymer are preferable. In particular, polyoxyethylene (EO average addition mole number 6) alkyl (carbon number 12-14) ether is more preferable.

The content of the surfactant in the cleaning composition of the present invention can be appropriately set by those skilled in the art. From the viewpoint of detergency and solubility, the content of the surfactant is preferably 10% by mass or more, more preferably 15% by mass or more, still more preferably 20% by mass or more, and 60% by mass in the total amount of the detergent composition. % Or less is preferable, 50 mass% or less is more preferable, and 45 mass% or less is more preferable. Or 10-60 mass% is preferable, 15-50 mass% is more preferable, 20-45 mass% is further more preferable. Among them, the content of the anionic surfactant is preferably 1% by mass or more, more preferably 2% by mass or more, further preferably 3% by mass or more, more preferably 60% by mass or less, and 50% by mass in the total amount of the detergent composition. % Or less is more preferable, and 40% by mass or less is more preferable. Or 1-60 mass% is preferable, 2-50 mass% is more preferable, and 3-40 mass% is further more preferable. Further, the content of the nonionic surfactant is preferably 1% by mass or more, more preferably 2% by mass or more, further preferably 4% by mass or more, preferably 45% by mass or less, and 35% by mass in the total amount of the detergent composition. % Or less is more preferable, and 25% by mass or less is more preferable. Or 1-45 mass% is preferable, 2-35 mass% is more preferable, and 4-25 mass% is further more preferable.
The anionic surfactant and the nonionic surfactant can be used alone, but are preferably used in combination. Moreover, you may use together an amphoteric surfactant and a cationic surfactant according to the objective.

(2) Builder A builder can be mix | blended with the cleaning composition of this invention.
Here, a “builder” is a component that can significantly improve its detergent ability when blended with a surfactant, even though it has no detergency or very weak detergency. means. As an effect | action of a builder, the multivalent metal cation capture | acquisition effect | action, stain | pollution | contamination dispersion | distribution effect | action, or an alkaline buffer effect | action, or those 2 or more types of combinations are mentioned, for example.
Examples of the builder include water-soluble inorganic compounds, water-insoluble inorganic compounds, and organic compounds.
Examples of water-soluble inorganic compound builders that can be used in the present invention include phosphates (tripolyphosphates, pyrophosphates, metaphosphates, trisodium phosphate, etc.), silicates, carbonates, sulfates, sulfites. Examples include salts. Among these, phosphate is preferable in that it has all of the action of capturing a polyvalent metal cation, the function of dispersing dirt, or the action of alkali buffering.
Examples of the builder of the water-insoluble inorganic compound that can be used in the present invention include aluminosilicate (A-type zeolite, P-type zeolite, X-type zeolite, amorphous aluminosilicate, etc.), crystalline silicate, and the like.
Examples of organic compound builders that can be used in the present invention include carboxylates (aminocarboxylates, hydroxyaminocarboxylates, hydroxycarboxylates, cyclocarboxylates, maleic acid derivatives, oxalates, etc.), organic Examples thereof include carboxylic acid (salt) polymers (acrylic acid polymers and copolymers, polyvalent carboxylic acid (eg, maleic acid and the like) polymers and copolymers, glyoxylic acid polymers, polysaccharides and salts thereof), and the like. Of these, organic carboxylic acid (salt) polymers are preferred.
In the builder's salt, the counter ion is preferably an alkali metal salt or an amine, particularly sodium, potassium, monoethanolamine or diethanolamine.
The builder that can be used in the present invention is preferably a water-soluble inorganic compound, a combination of a water-soluble inorganic compound and an organic compound, or a combination of a water-soluble inorganic compound, an organic compound and a water-insoluble inorganic compound.

  The content of the builder in the cleaning composition of the present invention can be appropriately set by those skilled in the art. For example, the builder content is preferably 20% by mass or more, more preferably 30% by mass or more, further preferably 35% by mass or more, more preferably 80% by mass or less, and more preferably 70% by mass in the total amount of the detergent composition. Preferably, 60 mass% or less is more preferable. Or 20-80 mass% is preferable, 30-70 mass% is more preferable, and 35-60 mass% is further more preferable. Among them, the water-soluble inorganic compound builder is preferably 10% by mass or more, more preferably 15% by mass or more, further preferably 20% by mass or more, more preferably 50% by mass or less, and more preferably 45% by mass in the total amount of the detergent composition. Preferably, 40 mass% or less is more preferable. Or 10-50 mass% is preferable, 15-45 mass% is more preferable, 20-40 mass% is further more preferable. Further, the water-insoluble inorganic compound builder is preferably 5% by mass or more, more preferably 10% by mass or more, further preferably 15% by mass or more, more preferably 50% by mass or less, and more preferably 45% by mass in the total amount of the detergent composition. Preferably, 40 mass% or less is more preferable. Or 5-50 mass% is preferable, 10-45 mass% is more preferable, 15-40 mass% is further more preferable. Further, the organic compound builder is preferably 0.1% by mass or more, more preferably 0.3% by mass or more, further preferably 0.5% by mass or more, and preferably 20% by mass or less in the total amount of the cleaning composition. More preferably, it is more preferably 10% by mass or less. Or 0.1-20 mass% is preferable, 0.3-15 mass% is more preferable, 0.5-10 mass% is further more preferable.

(3) Buffering agent A buffering agent can be mix | blended with the cleaning composition of this invention.
The pH of the buffer that can be used in the present invention is preferably 5.0 or more, more preferably 8.0 or more, and preferably 11.0 or less. Or pH 5.0-11.0 is preferable and pH 8.0-11.0 is more preferable. Further, the hardness of the buffer is preferably 0 degree.
Examples of the buffer that can be used in the present invention include Na citrate buffer (pH 5.0), Tris-HCl buffer (pH 8.0), glycine Na buffer (pH 11.0), and the like.

(4) Other components The detergent composition of the present invention includes an alkali agent (alkali metal carbonate, amorphous alkali metal silicate, sodium hydroxide, and mono, which is usually used in the field of detergent for textile products. Di- or triethanolamine), bleach (tetraacetylethylenediamine, etc.), fluorescent agent (biphenyl-type fluorescent agent, stilbene-type fluorescent agent, etc.), softener, reducing agent (sulfite, etc.), foam suppressor (silicone, etc.) ), Fragrance, various enzymes (cellulase, protease, amylase, lipase, etc.), and other components can be blended.

The detergent composition of the present invention can be produced by prescribing the transferrin family protein and, if necessary, any components usually used in detergents according to a conventional method.
The form of the cleaning composition of the present invention can be appropriately selected depending on the application. For example, it can be a liquid, powder, granule, paste, solid or the like. Among these, it is preferable that the form of the cleaning composition of the present invention is a liquid. In the present specification, “liquid” means having fluidity at 25 ° C. The fluidity can be measured according to a conventional method.

  When the textile product is washed using the detergent composition of the present invention, the final concentration of the transferrin family protein to be added is preferably 50 mg / L or more, more preferably 500 mg / L or more, preferably 4000 mg / L or less, more Preferably, it can be set appropriately so as to be 1000 mg / L or less.

By applying the cleaning composition of the present invention to a textile product according to a conventional method, it is possible to prevent the mud dirt adhering to the textile product from being washed and re-contamination of the textile product due to the mud dirt.
A cleaning method and a recontamination prevention method can be selected as appropriate. Specifically, it can be carried out by lay-in-place washing, hand washing, washing using a washing machine or a stirrer, and the like.

  The material of the textile product to which the cleaning composition of the present invention is applied is not particularly limited, and may be any of natural materials such as wool, silk and cotton, chemical fibers such as polyester and polyamide, and combinations thereof. . Of these, cotton, polyester, and combinations thereof are preferred.

  Regarding the above-described embodiments, the present invention further discloses the following detergent composition, method and use for textiles.

<1> A detergent composition for textiles, which contains a transferrin family protein.

<2> The detergent composition for textiles according to <1>, wherein the transferrin family protein is at least one glycoprotein selected from the group consisting of the following glycoproteins (a) to (i).

(A) a glycoprotein comprising a protein consisting of the amino acid sequence of SEQ ID NO: 1 (b) one or several, preferably 2-212, more preferably 2-142, still more preferably in the amino acid sequence of SEQ ID NO: 1. 2 to 71, more preferably 2 to 35, particularly preferably 2 to 7 amino acids in which the amino acid sequence is deleted, substituted, added, or inserted. (C) It consists of an amino acid sequence having a homology with the amino acid sequence of SEQ ID NO: 1 of 70% or more, preferably 80% or more, more preferably 90% or more, further preferably 95% or more, particularly preferably 99% or more. Glycoprotein (e) SEQ ID NO: 2 comprising a protein and comprising a protein consisting of the amino acid sequence of SEQ ID NO: 2 1 or several in the amino acid sequence, preferably 2 to 209, more preferably 2 to 140, still more preferably 2 to 70, still more preferably 2 to 35, particularly preferably 2 to 7 A protein comprising an amino acid sequence in which an amino acid is deleted, substituted, added or inserted, and has an iron-binding ability (f) The homology with the amino acid sequence of SEQ ID NO: 2 is 70% or more, preferably 80% or more More preferably 90% or more, further preferably 95% or more, particularly preferably 99% or more of a protein comprising an amino acid sequence and having an iron-binding ability (g) a protein comprising an amino acid sequence of SEQ ID NO: 3 1 or several, preferably 2 to 212, more preferably 2 to 1 in the amino acid sequence of SEQ ID NO: 3 Including a protein comprising an amino acid sequence in which one, more preferably 2 to 71, more preferably 2 to 35, particularly preferably 2 to 7 amino acids are deleted, substituted, added or inserted, and iron binding Glycoprotein (i) having a homology with the amino acid sequence of SEQ ID NO: 3 of 70% or more, preferably 80% or more, more preferably 90% or more, still more preferably 95% or more, particularly preferably 99% or more, Glycoprotein having an iron-binding ability

<3> When alignment of amino acid sequences is performed with reference to the amino acid sequence of SEQ ID NO: 1, the amino acid residue corresponding to position (x) in Table 1 in the transferrin family protein is (y) in Table 1 above. The detergent composition for textiles according to <1> or <2>, which is an amino acid residue shown in the above.
<4> The textile product according to any one of <1> to <3>, wherein the transferrin family protein is at least one glycoprotein selected from the group consisting of lactoferrin, serotransferrin, and ovotransferrin. Detergent composition.
<5> The detergent composition for textiles according to <4>, wherein the lactoferrin is bovine derived lactoferrin, preferably bovine milk derived lactoferrin.
<6> The detergent composition for textiles according to <4>, wherein the serotransferrin is human-derived serotransferrin, preferably human blood-derived serotransferrin.
<7> The detergent composition for textiles according to <4>, wherein the ovotransferrin is ovotransferrin derived from chicken egg white.
<8> The textile composition cleaning composition according to any one of <1> to <7>, wherein the textile composition is a liquid.
<9> The content of the transferrin family protein is 0.005% by mass or more, preferably 0.05% by mass or more, and 0.4% by mass or less, preferably in the total amount of the detergent composition for textiles. The cleaning composition for textiles according to any one of <1> to <8>, wherein is 0.2% by mass or less.
<10> The detergent composition for textiles according to any one of <1> to <9>, further including a surfactant.
<11> The content of the surfactant is 10% by mass or more, preferably 15% by mass or more, more preferably 20% by mass or more, and 60% by mass or less in the total amount of the detergent composition for textiles. The detergent composition for textiles according to <10>, preferably 50% by mass or less, more preferably 45% by mass or less.
<12> The fiber according to <10> or <11>, wherein the surfactant is at least one surfactant selected from the group consisting of an anionic surfactant and a nonionic surfactant. Product detergent composition.
<13> The anionic surfactant is a sulfate ester salt of an alcohol having 10 to 18 carbon atoms, a sulfate ester salt of an alkoxylated product of an alcohol having 8 to 20 carbon atoms, an alkylbenzene sulfonate salt, an alkyl sulfate ester salt, or paraffin. At least one anionic surfactant selected from the group consisting of sulfonate, α-olefin sulfonate, α-sulfo fatty acid salt, and α-sulfo fatty acid alkyl ester salt or fatty acid salt, preferably an alkyl chain It is at least one anionic surfactant selected from the group consisting of linear alkylbenzene sulfonates having 10 to 14 carbon atoms (more preferably 12 to 14 carbon atoms) and alkyl sulfates, Item 12> The cleaning composition for textiles.
<14> The content of the anionic surfactant is 1% by mass or more, preferably 2% by mass or more, more preferably 3% by mass or more, based on the total amount of the detergent composition for textiles. The cleaning composition for textiles according to <12> or <13>, wherein the cleaning composition is <10% by mass or less, preferably 50% by mass or less, more preferably 40% by mass or less.
<15> The nonionic surfactant is a polyoxyalkylene alkyl (carbon number 8 to 20) ether, alkyl polyglycoside, polyoxyalkylene alkyl (carbon number 8 to 20) phenyl ether, polyoxyalkylene sorbitan fatty acid (carbon Formula 8-22) At least one nonionic surfactant selected from the group consisting of an ester, a polyoxyalkylene glycol fatty acid (carbon number 8-22) ester, and a polyoxyethylene polyoxypropylene block polymer, preferably poly The cleaning composition for textiles according to <12>, which is oxyethylene (EO average addition mole number 6) alkyl (carbon number 12 to 14) ether.
<16> The content of the nonionic surfactant is 1% by mass or more, preferably 2% by mass or more, more preferably 4% by mass or more, based on the total amount of the detergent composition for textiles. The cleaning composition for textiles according to the above <12> or <15>, which is not more than mass%, preferably 35 mass%, more preferably not more than 25 mass%.

<17> Use of the detergent composition for textiles according to any one of <1> to <16> as a detergent for textiles.
<18> Use of the cleaning composition for textiles according to any one of <1> to <16> for the production of a textile cleaning agent.
<19> A method of using the textile product cleaning composition according to any one of <1> to <16> as a textile product cleaning agent.
<20> A method for cleaning mud stains of a textile product using the textile composition cleaning composition according to any one of <1> to <16>.
<21> A method for preventing re-contamination of a textile product due to mud stains, using the textile composition cleaning composition according to any one of <1> to <16>.
<22> Use of the cleaning composition for textiles according to any one of <1> to <16> for cleaning mud stains of textiles.
<23> Use of the cleaning composition for textiles according to any one of <1> to <16> for preventing recontamination of textiles due to mud stains.
<24> The use or method according to any one of <17> to <23>, wherein the material of the textile product is cotton, polyester, or a combination thereof.

  EXAMPLES Hereinafter, although this invention is demonstrated further in detail based on an Example, this invention is not limited to this.

Example 1
96-well deep well plates (trade name: CS40 (raw material: cotton); and trade name: PS40 (raw polyester), both obtained from Nippon Kagaku Co., Ltd.), each of which is cut into a diameter of 6 mm, are used in 96-well deep well plates. One was added to each well of Thermo, and 540 μL of 20 mM Tris-HCl (pH 8.0) buffer was added. Furthermore, bovine milk-derived lactoferrin (manufactured by Wako Pure Chemical Industries, Ltd.), human blood-derived serotransferrin (trade name: transferrin (Holo), manufactured by Wako Pure Chemical Industries, Ltd.), and egg white-derived ovotransferrin (brand names: Conalbumin, Wako Pure) 60 μL of each solution (solvent: 20 mM Tris-HCl, pH 8.0, hardness 0 degree) was added and adjusted appropriately so that the final concentration of each transferrin family protein was 0.5 g / L. As a control, 60 μL of deionized water was added instead of the protein solution.
Thereafter, the deep well plate was shaken for 24 hours under conditions of 370 rpm and 40 ° C. using a bioshaker (manufactured by Taitec). After shaking, the mixture was further shaken for 10 minutes with a high-speed shaker, and 250 μL of the washing solution was dispensed into a 96-well plate (manufactured by IWAKI).

Turbidity (OD ₆₀₀ ) in the washing solution was measured using a plate reader (Tecan). The mud free turbidity (ΔOD600) was calculated by subtracting the control turbidity value from the turbidity value in each washing solution. The higher the mud free turbidity, the higher the cleaning power of the cleaning composition against mud dirt.
The results are shown in Table 2.

  As is clear from the results in Table 2, the detergent composition for textiles containing transferrin family protein exhibits an excellent cleaning effect against mud soil adhering to the textiles.

Example 2
Sterile round bottom Spitz (Eiken Chemical Co., Ltd.) was charged with 50 mg of horticultural kanuma earth (Nichiri Nagase Co., Ltd.) and 10 mL of 20 mM Tris-HCl (pH 8.0). Here, deionized water (control), lactoferrin or oligomer D (manufactured by Kao Corporation, see JP-A-11-323390) is added to a final concentration of 1 g / L, and vortexed and mixed well. This time was set as 0 hour, and it left still at 40 degreeC. The turbidity (McFarland value) of the solution was measured by a McFarland turbidimetric method using a densitometer DEN-1B (trade name, manufactured by Wakenbee Tech Co., Ltd.) at regular intervals after mixing by vortex. The higher the McFarland value, the higher the turbidity of the solution, that is, the higher the mud dispersibility.
The results are shown in Table 3.

  As is apparent from the results in Table 3, the detergent composition for textiles containing the transferrin family protein exhibits the same dispersing effect on mud soil as that of the oligomer D.

Example 3
To the test tube, 250 mg of horticultural kanuma soil (manufactured by Nichiriu Nagase) and 10 mL of 20 mM Tris-HCl (pH 8.0) were added. Deionized water (control), lactoferrin, or oligomer D (manufactured by Kao Corporation, see JP-A-11-323390) was added thereto so that the final concentration was 1 g / L. A cotton cloth cut into 1 cm × 1 cm was put into each examiner, and after shaking at 37 ° C. and 250 spm for 30 minutes, the cloth was collected. The collected cloth was rinsed lightly, and the reflectance (460 nm) of the cloth was measured with a photometer (trade name: CM-2600d, manufactured by Konica Minolta), and the recontamination prevention rate was measured based on the following equation.

Recontamination prevention rate (%)
= [(Reflectance of white cotton cloth after test) / (Reflectance of white cotton cloth before test)] × 100

The closer this recontamination prevention rate is to 100%, the smaller the recontamination amount.
The results are shown in Table 4.

  As apparent from the results in Table 4, the detergent composition for textiles containing transferrin family protein shows an excellent preventive effect against the re-contamination of textiles due to mud soil, as compared with the oligomer D.

Claims (6)

  A detergent composition for textiles, comprising a transferrin family protein. The detergent composition for textiles according to claim 1, wherein the transferrin family protein is at least one glycoprotein selected from the group consisting of the following glycoproteins (a) to (i).
(A) a glycoprotein comprising a protein comprising the amino acid sequence of SEQ ID NO: 1 (b) comprising a protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, added or inserted in the amino acid sequence of SEQ ID NO: 1. A glycoprotein having iron binding ability (c) a protein consisting of an amino acid sequence having an amino acid sequence of 70% or more of the amino acid sequence of SEQ ID NO: 1 and having an iron binding ability (d) amino acid sequence of SEQ ID NO: 2 (E) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, added or inserted in the amino acid sequence of SEQ ID NO: 2 and having an iron-binding ability ( f) a protein comprising an amino acid sequence having a homology of 70% or more with the amino acid sequence of SEQ ID NO: 2 and having an iron binding ability Glycoprotein (g) comprising a protein consisting of the amino acid sequence of SEQ ID NO: 3 glycoprotein (h) consisting of an amino acid sequence in which one or several amino acids are deleted, substituted, added or inserted in the amino acid sequence of SEQ ID NO: 3 A glycoprotein containing an iron-binding ability (i) a glycoprotein having an iron-binding ability, comprising a protein consisting of an amino acid sequence having 70% or more homology with the amino acid sequence of SEQ ID NO: 3 When alignment of amino acid sequences is performed with reference to the amino acid sequence of SEQ ID NO: 1, the amino acid residue corresponding to position (x) in Table 1 below in the transferrin family protein is the amino acid shown in (y) in Table 1 below The detergent composition for textiles according to claim 1 or 2, which is a residue.

  The detergent composition for textiles according to any one of claims 1 to 3, wherein the transferrin family protein is at least one glycoprotein selected from the group consisting of lactoferrin, serotransferrin, and ovotransferrin.   The cleaning method of the mud stain of a textile product using the cleaning composition for textiles of any one of Claims 1-4. The method to prevent the re-contamination of the textiles by mud dirt using the cleaning composition for textiles of any one of Claims 1-4.

Images