JP2015139421A - Cultivation operation system - Google Patents

Cultivation operation system Download PDF

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JP2015139421A
JP2015139421A JP2014015188A JP2014015188A JP2015139421A JP 2015139421 A JP2015139421 A JP 2015139421A JP 2014015188 A JP2014015188 A JP 2014015188A JP 2014015188 A JP2014015188 A JP 2014015188A JP 2015139421 A JP2015139421 A JP 2015139421A
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decontamination
incubator
isolator
incubators
opening
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JP6354935B2 (en
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護 小久保
Mamoru Kokubo
護 小久保
壮馬 渡辺
Soma Watanabe
壮馬 渡辺
祐介 広沢
Yusuke Hirosawa
祐介 広沢
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Shibuya Corp
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Shibuya Kogyo Co Ltd
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Abstract

PROBLEM TO BE SOLVED: To provide a cultivation operation system having the following features that: the system comprises an isolator whose inner section is kept in a sterilized state by decontamination and a plurality of incubators capable of being communicated with the isolator; an operation required for cultivation is carried out in an inner section of the isolator; cultivation object is stored in the incubators communicated with the isolator; and cultivation is carried out for a predetermined period in a state where the incubators are separated from the isolator.SOLUTION: A cultivation operation system 1 comprises: an isolator 4 whose inner section is kept in a sterilized state; a plurality of incubators 3 capable of being communicated with the isolator 4; a storage chamber 2 storing the plurality of incubators 3; and an exclusive decontamination means 10 for decontamination of the inner section of the incubator 3 by communicating with the incubators 3 in the storage chamber 2. In the cultivation operation system 1, decontamination by the incubators 3 may be carried out independently from the isolator 4, thus the incubators 3 are may be subjected to the decontamination when the isolator 4 is used, resulting in improvement in working efficiency.

Description

本発明は培養作業システムに関し、より詳しくは、アイソレータと複数のインキュベータを備えるとともに、インキュベータの専用除染手段を備える培養作業システムに関する。   The present invention relates to a culture work system, and more particularly to a culture work system including an isolator and a plurality of incubators, and a dedicated decontamination means for the incubator.

従来、再生医療分野における培養作業システムにおいては、人体の細胞や組織を収容した培養容器を患者毎に複数のインキュベータ内に収納することにより、異なる複数の患者の細胞等を同時に培養することが行われている（例えば特許文献１、２）。
特許文献１の培養設備においては、培養容器を収納した移動容器を多数収容可能な培養室（インキュベータに相当）を備え、この培養室内で培養を行い、自在に走行する走行台車により移動容器を搬送して、培養容器を出し入れするようになっている。
また、特許文献２の採取物調製システムは、内部が除染されて無菌状態に維持されるアイソレータと、除染媒体を上記アイソレータの内部に供給して該アイソレータを除染する除染装置と、上記アイソレータに接続可能で相互に連通可能な複数のインキュベータとを備えており、上記アイソレータに１つのインキュベータを接続した状態で上記除染装置によりアイソレータを介してそれに接続されたインキュベータを除染できるようになっている。 Conventionally, in a culture work system in the field of regenerative medicine, by culturing a culture container containing human cells and tissues in a plurality of incubators for each patient, cells of a plurality of different patients can be cultured at the same time. (For example, Patent Documents 1 and 2).
The culture facility of Patent Document 1 includes a culture chamber (corresponding to an incubator) that can accommodate a large number of mobile containers that store culture containers, cultures in the culture chamber, and transports the mobile containers by a traveling carriage that freely runs. Then, the culture container is taken in and out.
Further, the collection preparation system of Patent Document 2 includes an isolator that is decontaminated and maintained in a sterile state, a decontamination device that supplies a decontamination medium to the inside of the isolator, and decontaminates the isolator, A plurality of incubators that can be connected to the isolator and communicate with each other, and the incubator connected thereto can be decontaminated by the decontamination device via the isolator in a state where one incubator is connected to the isolator. It has become.

特許第４２００４４４号公報Japanese Patent No. 4200434 特許第５１７７０８６号公報Japanese Patent No. 5177086

ところで、特許文献１の培養設備においては、培養室や移動容器の除染を実施するようにしておらず、異なる患者間でのクロスコンタミネーションが懸念される。これに対して、特許文献２の採取物調製システムでは、個々のインキュベータを除染するようしているが、各インキュベータを除染するに際して次のような問題点があった。つまり、特許文献２のシステムにおいては、インキュベータの使用に際してアイソレータにインキュベータを接続し、それらの内部を相互に連通させた状態において除染装置からアイソレータ内に除染媒体を供給してアイソレータとインキュベータを除染している。そのため、アイソレータ単体で除染する場合に比較して除染時間が長くなり、その間はアイソレータを使用できず迅速な作業の着手を阻害していた。特に、インキュベータ内は、細胞に影響がない程度に残留除染成分の濃度を低下させる必要があるが、そのための換気機能を備えていないため、アイソレータを介してインキュベータ内を除染する従来の処理方式においては、より一層除染時間が長くなるという問題があった。
さらに、従来のシステムにおいては次のような問題があった。つまり、由来の同じ細胞を複数のインキュベータに分けて収容する場合のように、アイソレータの使用中に異なるインキュベータを接続しようとしても、インキュベータの除染ができず対応できないという問題があった。 By the way, in the culture equipment of patent document 1, it does not carry out decontamination of a culture room or a moving container, and there is a concern about cross contamination between different patients. On the other hand, in the collection preparation system of Patent Document 2, each incubator is decontaminated. However, there are the following problems in decontaminating each incubator. That is, in the system of Patent Document 2, an incubator is connected to an isolator when the incubator is used, and a decontamination medium is supplied from the decontamination apparatus into the isolator in a state where the insides are in communication with each other. It is decontaminated. For this reason, the decontamination time is longer than in the case of decontamination with a single isolator, and during that period, the isolator cannot be used, which hinders the rapid start of work. In particular, in the incubator, it is necessary to reduce the concentration of residual decontamination components to the extent that there is no effect on the cells, but since there is no ventilation function for that purpose, conventional treatment that decontaminates the inside of the incubator via an isolator The method has a problem that the decontamination time is further increased.
Further, the conventional system has the following problems. That is, there is a problem that even if different incubators are connected during use of the isolator, the incubator cannot be decontaminated and cannot be handled as in the case where the same cells derived from are accommodated in a plurality of incubators.

上述した事情に鑑み、本発明は、内部が除染されて無菌状態に維持されるアイソレータと、アイソレータに接続可能な複数のインキュベータを備え、アイソレータの内部で培養に要する作業を行うとともに、アイソレータに接続させたインキュベータに被培養物を収納し、インキュベータをアイソレータから切り離して所定期間にわたって培養を行う培養作業システムにおいて、
上記複数のインキュベータを保管する保管室と、該保管室内のインキュベータと接続してインキュベータの内部を除染する専用除染手段を備え、
上記保管室には、被培養物を収納した培養中のインキュベータと、上記専用除染手段で除染された空のインキュベータが保管されることを特徴とするものである。 In view of the circumstances described above, the present invention includes an isolator that is decontaminated and maintained in a sterile state, and a plurality of incubators that can be connected to the isolator, and performs operations required for culture inside the isolator. In a culture work system in which a culture object is stored in a connected incubator, and the incubator is separated from the isolator and cultured for a predetermined period of time,
A storage room for storing the plurality of incubators, and a dedicated decontamination means for decontaminating the inside of the incubator by connecting to the incubator in the storage room;
The storage room stores an incubator in culture containing the culture object and an empty incubator decontaminated by the dedicated decontamination means.

このような構成によれば、インキュベータの除染をアイソレータとは独立して行うことができるため、アイソレータの使用中もインキュベータを除染することができ、作業効率を向上させることができる。   According to such a configuration, since the decontamination of the incubator can be performed independently of the isolator, the incubator can be decontaminated during the use of the isolator, and the working efficiency can be improved.

本発明の一実施例を示す概略の平面図。1 is a schematic plan view showing an embodiment of the present invention. インキュベータの保管室の側壁の開口部を示す斜視図。The perspective view which shows the opening part of the side wall of the storage chamber of an incubator. インキュベータを示す斜視図。The perspective view which shows an incubator. インキュベータを開口部に接続した状態を示す斜視図。The perspective view which shows the state which connected the incubator to the opening part. 図１に示した実施例による作業工程図であり、図５（ａ）は最初のインキュベータを専用除染手段に連結して除染する工程を示し、 図５（ｂ）及び図５（ｃ）は残りの各インキュベータを専用除染手段に順次連結して除染する工程を示し、図５（ｄ）はアイソレータにインキュベータを接続する工程を示している。FIG. 5 (a) shows a process of decontamination by connecting the first incubator to a dedicated decontamination means, and FIG. 5 (b) and FIG. 5 (c). Shows the step of connecting the remaining incubators sequentially to the dedicated decontamination means for decontamination, and FIG. 5 (d) shows the step of connecting the incubator to the isolator.

以下、図示実施例について本発明を説明すると、図１において１は、再生医療のために人体の細胞や組織の培養を行う培養作業システムである。この培養作業システム１は、内部が除染されて無菌状態に維持されるアイソレータ４と、このアイソレータ４に接続可能な複数のインキュベータ３（３Ａ〜３Ｈ）を備えており、さらに、密閉された部屋からなるインキュベータ３（３Ａ〜３Ｈ）の保管室２と、この保管室２のインキュベータ３を接続させてインキュベータ３の内部を除染するインキュベータ３の専用除染手段１０が設けられている。保管室２の外部にアイソレータ４が配置されるとともに、保管室２の側壁２Ａの第１開口部２Ｂを介して保管室２の内部と連通可能となっており、保管室２の外部にはさらに除染手段５が配置され、アイソレータ４と保管室２内に除染媒体を供給してそれらの内部を除染するようになっている。また、専用除染手段１０は、保管室２の外部に配置されるとともに、保管室２の側壁２Ａの第２開口部２Ｃを介して保管室２内のインキュベータ３と接続可能となっている。また、図２に示すように、側壁２Ａの外面２Ａａに、第１開口部２Ｂを囲繞する外枠２Ｄが設けられ、この外枠２Ｄにはアイソレータ４内に開放される第１扉２Ｅが設けられている。また同様に外面２Ａａにおいて、第２開口部２Ｃを囲繞する外枠２Ｆには専用除染手段１０側に開放される第２扉２Ｇが設けられている。   Hereinafter, the present invention will be described with reference to the illustrated embodiments. In FIG. 1, reference numeral 1 denotes a culture work system for culturing human cells and tissues for regenerative medicine. The culture work system 1 includes an isolator 4 whose interior is decontaminated and maintained in a sterile state, and a plurality of incubators 3 (3A to 3H) that can be connected to the isolator 4, and is a sealed room. A storage chamber 2 of the incubator 3 (3A to 3H) is provided, and a dedicated decontamination means 10 for the incubator 3 for connecting the incubator 3 of the storage chamber 2 to decontaminate the inside of the incubator 3 is provided. The isolator 4 is disposed outside the storage chamber 2 and can communicate with the inside of the storage chamber 2 through the first opening 2B of the side wall 2A of the storage chamber 2. A decontamination means 5 is arranged, and a decontamination medium is supplied into the isolator 4 and the storage chamber 2 to decontaminate the inside thereof. The dedicated decontamination means 10 is arranged outside the storage chamber 2 and can be connected to the incubator 3 in the storage chamber 2 through the second opening 2C of the side wall 2A of the storage chamber 2. Further, as shown in FIG. 2, an outer frame 2D surrounding the first opening 2B is provided on the outer surface 2Aa of the side wall 2A, and a first door 2E opened in the isolator 4 is provided on the outer frame 2D. It has been. Similarly, on the outer surface 2Aa, the outer frame 2F surrounding the second opening 2C is provided with a second door 2G that is opened to the dedicated decontamination means 10 side.

インキュベータ３は、図３に示すように、同一寸法で立方体の箱形に形成されており、各インキュベータ３正面の開閉部３ａには開口部３ｂを開閉する正方形の開閉扉３ｃが設けられている。この開閉扉３ｃが開閉されることで、各インキュベータ３は接続されたアイソレータ４との間で、被培養物として細胞や組織を収容した培養容器９を出し入れできるようになっている。培養容器９が各インキュベータ３内に収容された後に開閉扉３ａを閉鎖するとインキュベータ３内を密閉できるようになっている。
本実施例では合計８個のインキュベータ３Ａ〜３Ｈが、保管室２の後方側の側壁２Ｈに近い保管領域ＳＡに前後２列で隣接させて保管されるようになっている。保管領域ＳＡには、各インキュベータ３Ａ〜３Ｈに対応させてそれらを収容する収容部ＳＡ１〜ＳＡ８が形成されており、これらの各収容部ＳＡ１〜ＳＡ８内にそれらに対応する各インキュベータ３Ａ〜３Ｈが収容されて保管されるようになっている。保管領域ＳＡの各収容部ＳＡ１〜ＳＡ８には、保管室２の外部から側壁２Ｈを貫通させて引き込まれた気体の供給管１１と排出管１２の各分岐端が接続されている。なお、供給管１１、排出管１２の分岐端は、側壁２Ｈに近い後列の収容部ＳＡ２、ＳＡ４、ＳＡ６、ＳＡ８への接続を図示し、前列ＳＡ１、ＳＡ３、ＳＡ５、ＳＡ７への接続の図示を省略している。
そして、保管領域ＳＡの各収容部ＳＡ１〜ＳＡ８内にそれらに対応する各インキュベータ３Ａ〜３Ｈが収容されると、各インキュベータ３Ａ〜３Ｈの内部と上記供給管１１と排出管１２の分岐端が連通するようになっている。この状態において、図示しない気体の給排手段は、上記供給管１１と排出管１２とを介して各収容部ＳＡ１〜ＳＡ８内に収容された各インキュベータ３Ａ〜３Ｈ内に必要な気体を給排できるようになっている。なお、上述した保管領域ＳＡの収容部ＳＡ１〜ＳＡ８と各インキュベータ３Ａ〜３Ｈの構成及び供給管１１、排出管１２に関する構成は上記特許文献２により公知であるため、ここでの詳細な説明は省略する。 As shown in FIG. 3, the incubator 3 is formed in a cubic box shape having the same dimensions, and a square opening / closing door 3 c that opens and closes the opening 3 b is provided in the opening / closing portion 3 a in front of each incubator 3. . By opening and closing the open / close door 3c, each incubator 3 can take in and out a culture vessel 9 containing cells and tissues as an object to be cultured with the connected isolator 4. When the opening / closing door 3a is closed after the culture container 9 is accommodated in each incubator 3, the inside of the incubator 3 can be sealed.
In the present embodiment, a total of eight incubators 3 </ b> A to 3 </ b> H are stored adjacent to each other in two rows in the front and back in the storage area SA near the side wall 2 </ b> H on the rear side of the storage chamber 2. In the storage area SA, accommodating portions SA1 to SA8 for accommodating the respective incubators 3A to 3H are formed, and in the respective accommodating portions SA1 to SA8, the respective incubators 3A to 3H corresponding to them are formed. It is housed and stored. Respective branch ends of a gas supply pipe 11 and a discharge pipe 12 drawn through the side wall 2H from the outside of the storage chamber 2 are connected to the storage portions SA1 to SA8 of the storage area SA. It should be noted that the branch ends of the supply pipe 11 and the discharge pipe 12 illustrate the connection to the rear row storage portions SA2, SA4, SA6, and SA8 close to the side wall 2H, and illustrate the connection to the front row SA1, SA3, SA5, and SA7. Omitted.
When the corresponding incubators 3A to 3H are accommodated in the accommodating portions SA1 to SA8 of the storage area SA, the insides of the incubators 3A to 3H and the branch ends of the supply pipe 11 and the discharge pipe 12 communicate with each other. It is supposed to be. In this state, a gas supply / exhaust means (not shown) can supply and discharge the necessary gas into each of the incubators 3A to 3H accommodated in each of the accommodating portions SA1 to SA8 via the supply pipe 11 and the exhaust pipe 12. It is like that. Note that the configurations of the storage units SA1 to SA8 of the storage area SA and the incubators 3A to 3H and the configuration related to the supply pipe 11 and the discharge pipe 12 are known from the above-mentioned Patent Document 2, and therefore detailed description thereof is omitted here. To do.

上記保管室２の内部には、図示しない自走式台車からなる搬送装置が設けられている。この搬送装置によって各インキュベータ３Ａ〜３Ｈは保管領域ＳＡの上記収容部ＳＡ１〜ＳＡ８の位置と、アイソレータ４に隣接する第１開口部２Ｂの位置と、専用除染手段１０に隣接する第２開口部２Ｃの位置とにわたって搬送されるようになっている。保管室２内に配置された図示しない搬送装置の作動は制御装置８によって制御されるようになっている。制御装置８は搬送装置の作動を制御して各インキュベータ３Ａ〜３Ｈを上記各保管領域ＳＡと第１開口部２Ｂの位置と第２開口部２Ｃの位置とにわたって移動させるようになっている。なお、インキュベータ３を搬送する搬送装置としては、例えば特許文献１に開示されている移動容器を搬送する走行台車を用いることができる。また、無菌スーツを着用した作業者が人手により搬送するようにしてもよい。 Inside the storage chamber 2 is provided a transfer device composed of a self-propelled carriage (not shown). With this conveying device, each incubator 3A-3H has a position of the storage parts SA1-SA8 in the storage area SA, a position of the first opening 2B adjacent to the isolator 4, and a second opening adjacent to the dedicated decontamination means 10. It is transported over the 2C position. The operation of a transfer device (not shown) arranged in the storage chamber 2 is controlled by the control device 8. The control device 8 controls the operation of the transport device to move the incubators 3A to 3H across the storage areas SA, the positions of the first openings 2B, and the positions of the second openings 2C. In addition, as a conveying apparatus which conveys the incubator 3, the traveling cart which conveys the moving container currently disclosed by patent document 1 can be used, for example. Moreover, you may make it the operator who wore the aseptic suit convey manually.

保管室２の側壁２Ａの外面２Ａａには、第１開口部２Ｂを内部に位置させた状態でアイソレータ４が気密を保持して連結されている。アイソレータ４の内部は、除染後は外部より陽圧に管理され無菌状態に維持され、無菌環境での作業が可能となっている。アイソレータ４には複数の作業用グローブ１３が設けられている。そして、作業者が上記作業用グローブ１３に手を差し込んで、アイソレータ４の内部で培養に必要な作業を行うとともに第１扉２Ｅを開いて、接続されているインキュベータ３の開閉扉３ｃを開くことができるようになっている。
アイソレータ４にはさらに図示しないトランスファポートやパスボックスが設けられており、アイソレータ４内に培養容器９等を搬入できるようになっている。そして、前述した保管室２内の搬送装置によって例えば１つのインキュベータ３が第１開口部２Ｂまで搬送されてくると、該インキュベータ３の開閉部３ａを第１開口部２Ｂに差し込むようになっており、両者間にシール部材を介在させることにより、インキュベータ３がアイソレータ４に気密を保持して接続されるようになっている。そして、この接続状態となると、作業用グローブ１３に作業者が手を差し込んで、第１扉２Ｅを開いて（図４（ａ）参照）、インキュベータ３の開閉扉３ｃを開放させるようになっている（図４（ｂ）参照）。それにより、アイソレータ４内とインキュベータ３内とが連通するので、この連通状態において、作業者は、アイソレータ４内で操作を終えた培養容器９をインキュベータ３内に収容し、開閉扉３ｃを閉鎖するようになっている。 An isolator 4 is connected to the outer surface 2Aa of the side wall 2A of the storage chamber 2 while keeping the first opening 2B inside to keep airtight. The inside of the isolator 4 is managed at a positive pressure from the outside after decontamination and is maintained in a sterilized state, so that the work in an aseptic environment is possible. The isolator 4 is provided with a plurality of work gloves 13. Then, the operator inserts his / her hand into the work glove 13 to perform the work necessary for the culture inside the isolator 4 and opens the first door 2 </ b> E to open the open / close door 3 c of the connected incubator 3. Can be done.
The isolator 4 is further provided with a transfer port and a pass box (not shown) so that the culture vessel 9 and the like can be carried into the isolator 4. When, for example, one incubator 3 is transported to the first opening 2B by the above-described transport device in the storage chamber 2, the opening / closing part 3a of the incubator 3 is inserted into the first opening 2B. The incubator 3 is connected to the isolator 4 in an airtight manner by interposing a seal member therebetween. And when it will be in this connection state, an operator will insert a hand in the work glove 13, will open the 1st door 2E (refer Fig.4 (a)), and will come to open the opening-and-closing door 3c of the incubator 3. (See FIG. 4B). As a result, the inside of the isolator 4 and the inside of the incubator 3 communicate with each other, and in this communication state, the operator houses the culture vessel 9 that has finished the operation in the isolator 4 in the incubator 3 and closes the open / close door 3c. It is like that.

除染手段５は、過酸化水素蒸気等の気体からなる除染媒体を供給する除染媒体供給手段１４を備えるとともに、アイソレータ４内と保管室２内に除染媒体供給手段１４を接続する給排管１５とを備えている。そして、除染媒体供給手段１４から給排管１５を介してアイソレータ４の内部と保管室２の内部に除染媒体を供給できるようになっている。
一方、本実施例の専用除染手段１０は密閉された立方体状に形成された除染チャンバ６を備えており、この除染チャンバ６を上記第２開口部２Ｃを内部に位置させた状態で気密を保持して側壁２Ａの外面２Ａａに連結させている。除染チャンバ６には作業用グローブ１６が設けられている。
専用除染手段１０は、さらに過酸化水素蒸気等の気体からなる除染媒体を供給する除染媒体供給手段２１と除染チャンバ６と除染媒体供給手段２１とを接続する給排管２２とを備えている。そして、除染媒体供給手段２１から給排管２２を介して除染チャンバ６の内部に除染媒体を供給できるようになっている。
前述した保管室２内の搬送装置によって１つのインキュベータ３が第２開口部２Ｃの位置まで搬送されてくると、該インキュベータ３の開閉部３ａを第２開口部２Ｃに差し込むようになっており、両者間にシール部材を介在させることにより、インキュベータ３が専用除染手段１０の除染チャンバ６に気密を保持して接続されるようになっている。そして、この接続状態となると、上記作業用グローブ１６に作業者が手を差し込んで、第２扉２Ｇを開くことで（図４（ａ）参照）、開閉部３ａは除染チャンバ６に直接的に覆われた状態となり、その後インキュベータ３の開閉扉３ｃを開放させるようになっている（図４（ｂ）参照）。それにより、除染チャンバ６内とそれに接続されたインキュベータ３Ａ内が相互に連通するようになっている。この連通状態において、除染媒体供給手段２１から除染チャンバ６内に除染媒体が供給されて、インキュベータ３の内部と開閉扉３ｃや開閉部３ａの密封箇所３ｄが除染されるようになっている。なお、このように第２開口部２Ｃは、第１開口部２Ｂと、共通の構成を備えている。 The decontamination means 5 includes a decontamination medium supply means 14 for supplying a decontamination medium made of a gas such as hydrogen peroxide vapor, and a supply for connecting the decontamination medium supply means 14 in the isolator 4 and the storage chamber 2. And an exhaust pipe 15. The decontamination medium can be supplied from the decontamination medium supply means 14 to the inside of the isolator 4 and the inside of the storage chamber 2 through the supply / discharge pipe 15.
On the other hand, the dedicated decontamination means 10 of this embodiment includes a decontamination chamber 6 formed in a sealed cubic shape, and the decontamination chamber 6 is located with the second opening 2C positioned inside. Airtight is maintained and connected to the outer surface 2Aa of the side wall 2A. A work glove 16 is provided in the decontamination chamber 6.
The dedicated decontamination means 10 further includes a decontamination medium supply means 21 for supplying a decontamination medium made of a gas such as hydrogen peroxide vapor, a supply / discharge pipe 22 for connecting the decontamination chamber 6 and the decontamination medium supply means 21. It has. The decontamination medium can be supplied into the decontamination chamber 6 from the decontamination medium supply means 21 via the supply / discharge pipe 22.
When one incubator 3 is transported to the position of the second opening 2C by the transport device in the storage chamber 2 described above, the opening / closing part 3a of the incubator 3 is inserted into the second opening 2C, By interposing a seal member therebetween, the incubator 3 is connected to the decontamination chamber 6 of the dedicated decontamination means 10 while maintaining airtightness. When this connection state is established, the operator inserts his / her hand into the work glove 16 and opens the second door 2G (see FIG. 4A), so that the opening / closing portion 3a directly enters the decontamination chamber 6. Then, the door 3c of the incubator 3 is opened (see FIG. 4B). As a result, the inside of the decontamination chamber 6 and the inside of the incubator 3A connected thereto are communicated with each other. In this communication state, the decontamination medium is supplied from the decontamination medium supply means 21 into the decontamination chamber 6, and the inside of the incubator 3 and the sealing portion 3d of the open / close door 3c and the open / close part 3a are decontaminated. ing. As described above, the second opening 2C has the same configuration as the first opening 2B.

以上のように構成された本実施例の培養作業システム１による細胞の培養作業及び事前の各部の除染作業は以下のようにして行われる。
すなわち、先ず、図１に示すように開閉扉３ｃを閉鎖された各インキュベータ３Ａ〜３Ｈが保管領域ＳＡの所定の収容部ＳＡ１〜ＳＡ８内に収容された状態で、第１扉２Ｅは開放した状態とし、アイソレータ４内及び保管室２内に除染手段５から給排管１５を介して除染媒体として過酸化水素蒸気を供給する。これにより第１開口部２Ｂの周囲の外枠２Ｄと第１扉２Ｅとの密封箇所を含むアイソレータ４の内部及び保管室２の内部空間が除染される。また、各インキュベータ３Ａ〜３Ｈの外面や供給管１１及び排出管１２の内部も除染されるようになっている。
アイソレータ４内の除染が終了すると、アイソレータ４内で培養のための準備作業を開始することができる。 The cell culturing operation and the prior decontamination operations of the respective parts by the culturing operation system 1 of the present embodiment configured as described above are performed as follows.
That is, first, as shown in FIG. 1, the first door 2E is opened in a state where the incubators 3A to 3H with the open / close door 3c closed are accommodated in the predetermined accommodating portions SA1 to SA8 of the storage area SA. Then, hydrogen peroxide vapor is supplied as a decontamination medium from the decontamination means 5 into the isolator 4 and the storage chamber 2 through the supply / discharge pipe 15. As a result, the interior of the isolator 4 and the internal space of the storage chamber 2 including the sealed portion between the outer frame 2D around the first opening 2B and the first door 2E are decontaminated. Further, the outer surfaces of the incubators 3A to 3H and the inside of the supply pipe 11 and the discharge pipe 12 are also decontaminated.
When the decontamination in the isolator 4 is completed, the preparatory work for culture can be started in the isolator 4.

他方、保管室２内の収容部ＳＡ１〜ＳＡ８に収容された各インキュベータ３Ａ〜３Ｈは、それらの開閉扉３ｃが閉鎖されているので、その状態では各インキュベータ３Ａ〜３Ｈ内部を除染できない。そこで、除染手段５によるアイソレータ４と保管室２内の除染が終了すると、以下のようにして専用除染手段１０によって各インキュベータ３Ａ〜３Ｈ内の除染が行われる。すなわち、前述した保管室２内の搬送装置によって、第１のインキュベータ３Ａが除染チャンバ６に隣接する第２開口部２Ｃの位置まで搬送されて接続される（図５（ａ）参照）。この状態で作業者は除染チャンバ６の作業用グローブ１６を用いて第２扉２Ｇを開いて（図４（ａ）参照）、インキュベータ３Ａの開閉扉３ｃを開放させるので（図４（ｂ）参照）、該インキュベータ３Ａ内と除染チャンバ６内とが相互に連通する。その後、除染媒体供給手段２１から給排管２２を介して除染チャンバ６に除染媒体として過酸化水素蒸気が供給される。これにより、除染チャンバ６からインキュベータ３Ａ内にも除染媒体が供給されて、インキュベータ３Ａの内部と開閉部３ａの密封箇所３ｄが除染される。 On the other hand, since each of the incubators 3A to 3H accommodated in the accommodating portions SA1 to SA8 in the storage chamber 2 has their open / close door 3c closed, the inside of the incubators 3A to 3H cannot be decontaminated in that state. Therefore, when the decontamination in the isolator 4 and the storage chamber 2 by the decontamination unit 5 is completed, the decontamination in the incubators 3A to 3H is performed by the dedicated decontamination unit 10 as follows. That is, the first incubator 3A is transported to the position of the second opening 2C adjacent to the decontamination chamber 6 by the transport device in the storage chamber 2 described above (see FIG. 5A). In this state, the operator opens the second door 2G using the work glove 16 in the decontamination chamber 6 (see FIG. 4A) and opens the open / close door 3c of the incubator 3A (FIG. 4B). The incubator 3A and the decontamination chamber 6 communicate with each other. Thereafter, hydrogen peroxide vapor is supplied as a decontamination medium from the decontamination medium supply means 21 to the decontamination chamber 6 through the supply / discharge pipe 22. As a result, the decontamination medium is also supplied from the decontamination chamber 6 into the incubator 3A, and the inside of the incubator 3A and the sealed portion 3d of the opening / closing part 3a are decontaminated.

その後、作業者が作業用グローブ１６を介してインキュベータ３Ａの開閉扉３ｃを閉鎖するとともに第２扉２Ｇを閉じる。その後、図示しない搬送装置によって第１のインキュベータ３Ａが第２開口部２Ｃから切り離されて元の収容部ＳＡ１まで搬送されて収容されるとともに、第２のインキュベータ３Ｂが搬送装置によって収容部ＳＡ２から第２開口部２Ｃの位置まで搬送されて連結される（図５（ｂ）参照）。この第２のインキュベータ３についても同様に除染される。   Thereafter, the operator closes the open / close door 3c of the incubator 3A via the work glove 16 and closes the second door 2G. Thereafter, the first incubator 3A is separated from the second opening 2C by the transport device (not shown), transported to the original storage portion SA1, and stored, and the second incubator 3B is moved from the storage portion SA2 by the transport device. It is transported and connected to the position of the two openings 2C (see FIG. 5B). This second incubator 3 is similarly decontaminated.

以下、同様に上記搬送装置により残りのインキュベータ３Ｃ〜３Ｈを順次第２開口部２Ｃの位置へ搬送して、各インキュベータ３Ｃ〜３Ｈの除染が順次行われる（図５（ｃ）参照）。
以上のようにして保管室２内の８個全てのインキュベータ３Ａ〜３Ｈ内の除染が終了し、各インキュベータ３Ａ〜３Ｈが保管領域ＳＡの所定の収容部ＳＡ１〜ＳＡ８に収容された状態となる。
以上のようにしてインキュベータ３は、気密を保持して第２開口部２Ｃに接続され、除染チャンバ６で開放され除染されるため、培養していた細胞が保有していた菌やウィルスが保管室２内に拡散することがなくクロスコンタミネーションが防止される。 Thereafter, similarly, the remaining incubators 3C to 3H are sequentially transported to the position of the second opening 2C by the transport device, and decontamination of the incubators 3C to 3H is sequentially performed (see FIG. 5C).
As described above, decontamination in all eight incubators 3A to 3H in the storage chamber 2 is completed, and the respective incubators 3A to 3H are accommodated in the predetermined accommodating portions SA1 to SA8 in the storage area SA. .
As described above, the incubator 3 is connected to the second opening 2C while maintaining airtightness, and is opened and decontaminated in the decontamination chamber 6, so that the bacteria and viruses possessed by the cultured cells are retained. Cross contamination is prevented without diffusing into the storage chamber 2.

インキュベータ３の除染処理と並行してアイソレータ４では作業が行われている。
アイソレータ４内での作業が終了すると、培養容器９をインキュベータ３に移して培養を開始する。この場合には、先ず搬送装置によって最初に除染されて除染成分が十分に消滅した状態で保管されている空のインキュベータ３Ａを収容部ＳＡ１から第１開口部２Ｂの位置まで搬送し、第１開口部２Ｂに気密を保持して接続する（図５（ｄ）参照）。その後、作業者はアイソレータ４の作業グローブ１３に手を差し込んで第１扉２Ｅを開き（図４（ａ）参照）、インキュベータ３Ａの開閉扉３ｃを開放させ（図４（ｂ）参照）、培養容器９を収めて開閉扉３ａを閉鎖する。
この後、培養容器９が収められたインキュベータ３Ａは、上記搬送装置により第１開口部２Ｂから切り離された後に、元の収容部ＳＡ１の位置まで搬送されて収容される。該インキュベータ３Ａが収容部ＳＡ１に収容されると、供給管１１と排出管１２が接続され、供給管１１を介して該インキュベータ３Ａ内に気体が供給されて、培養容器９に収納された被培養物が培養されるようになっている。
一方、このように、インキュベータ３をアイソレータ４に接続している状態においても、別のインキュベータ３の除染を継続することができる。また、培養を終了したインキュベータ３から順に除染することも可能である。 Work is performed in the isolator 4 in parallel with the decontamination processing of the incubator 3.
When the work in the isolator 4 is completed, the culture vessel 9 is moved to the incubator 3 and culture is started. In this case, the empty incubator 3A first decontaminated by the conveying device and stored in a state where the decontaminated components have sufficiently disappeared is conveyed from the housing part SA1 to the position of the first opening 2B, It connects to 1 opening part 2B, maintaining airtightness (refer FIG.5 (d)). Thereafter, the operator inserts his hand into the work glove 13 of the isolator 4 to open the first door 2E (see FIG. 4 (a)), and opens the open / close door 3c of the incubator 3A (see FIG. 4 (b)). The container 9 is accommodated and the open / close door 3a is closed.
Thereafter, the incubator 3A in which the culture vessel 9 is stored is separated from the first opening 2B by the transport device, and then transported and stored to the position of the original storage unit SA1. When the incubator 3A is accommodated in the accommodating part SA1, the supply pipe 11 and the discharge pipe 12 are connected, and the gas is supplied into the incubator 3A via the supply pipe 11 and is stored in the culture vessel 9. Things are cultivated.
On the other hand, even in a state where the incubator 3 is connected to the isolator 4 as described above, decontamination of another incubator 3 can be continued. Moreover, it is also possible to decontaminate sequentially from the incubator 3 that has completed the culture.

以上のように、本実施例の培養作業システム１は、アイソレータ４とは別に各インキュベータ３Ａ〜３Ｈを個別に除染するための専用除染手段１０を備えている。そのため、アイソレータ４での作業と並行してインキュベータ３の除染を行うことができる。また、培養を終えたインキュベータ３から順次、次回の使用に備えて除染して除染済の状態で保管しておくことができるので、その間に内部に残留する除染成分を十分に消滅させることができる。
また、保管室２の内部や供給管１１や排出管１２等の接続配管内部およびインキュベータ３の外面も除染することができるため、培養中の雑菌の侵入や由来の異なる細胞間でのクロスコンタミネーションが防止される。 As described above, the culture work system 1 according to the present embodiment includes the dedicated decontamination means 10 for decontaminating each of the incubators 3A to 3H separately from the isolator 4. Therefore, decontamination of the incubator 3 can be performed in parallel with the work with the isolator 4. Moreover, since it can be decontaminated and stored in a decontaminated state sequentially for the next use from the incubator 3 after culturing, the decontaminating components remaining in the inside are sufficiently extinguished. be able to.
In addition, since the inside of the storage chamber 2, the inside of the connecting pipe such as the supply pipe 11 and the discharge pipe 12, and the outer surface of the incubator 3 can be decontaminated, invasion of germs during culturing and cross contamination between cells of different origins are possible. Nation is prevented.

１‥培養作業システム ２‥保管室
３‥インキュベータ ４‥アイソレータ
５‥除染手段
１０‥専用除染手段 1. Incubation system 2. Storage room 3. Incubator 4. Isolator 5. Decontamination means 10. Dedicated decontamination means

Claims (4)

内部が除染されて無菌状態に維持されるアイソレータと、アイソレータに接続可能な複数のインキュベータを備え、アイソレータの内部で培養に要する作業を行うとともに、アイソレータに接続させたインキュベータに被培養物を収納し、インキュベータをアイソレータから切り離して所定期間にわたって培養を行う培養作業システムにおいて、
上記複数のインキュベータを保管する保管室と、該保管室内のインキュベータと接続してインキュベータの内部を除染する専用除染手段を備え、
上記保管室には、被培養物を収納した培養中のインキュベータと、上記専用除染手段で除染された空のインキュベータが保管されることを特徴とする培養作業システム。 Equipped with an isolator that is decontaminated and maintained in a sterile state, and multiple incubators that can be connected to the isolator, perform the work required for culturing inside the isolator, and store the culture in the incubator connected to the isolator In the culture work system in which the incubator is separated from the isolator and cultured for a predetermined period of time,
A storage room for storing the plurality of incubators, and a dedicated decontamination means for decontaminating the inside of the incubator by connecting to the incubator in the storage room;
A culture work system characterized in that the storage chamber stores a culture incubator containing a culture object and an empty incubator decontaminated by the dedicated decontamination means. 上記アイソレータは保管室の外部に設置され、保管室の側壁にインキュベータをアイソレータに接続させるための開口部が設けられていることを特徴とする請求項１に記載の培養作業システム。   The culture work system according to claim 1, wherein the isolator is installed outside the storage chamber, and an opening for connecting the incubator to the isolator is provided on a side wall of the storage chamber. 上記専用除染手段は、インキュベータの開閉部を覆う除染チャンバと、除染チャンバ内に除染媒体を供給する除染媒体供給手段を備え、
上記除染チャンバ内でインキュベータの開閉部を開放させるとともに、除染チャンバ内に除染媒体を供給してインキュベータの内部と開放された上記開閉部の密封箇所を除染することを特徴とする請求項１または２に記載の培養作業システム。 The dedicated decontamination means comprises a decontamination chamber that covers the opening / closing part of the incubator, and a decontamination medium supply means for supplying a decontamination medium into the decontamination chamber,
The opening and closing part of the incubator is opened in the decontamination chamber, and a decontamination medium is supplied into the decontamination chamber to decontaminate the inside of the incubator and the opened sealing part of the opening and closing part. Item 3. The culture work system according to Item 1 or 2. 上記除染チャンバは保管室の外部に設置され、保管室の側壁にインキュベータの開閉部を除染チャンバ内に臨ませる第２の開口部が設けられていることを特徴とする請求項３に記載の培養作業システム。   The decontamination chamber is installed outside the storage room, and a second opening is provided on the side wall of the storage room so that the opening / closing part of the incubator faces the decontamination chamber. Culture work system.
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