JP2015125084A5 - - Google Patents
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- JP2015125084A5 JP2015125084A5 JP2013270762A JP2013270762A JP2015125084A5 JP 2015125084 A5 JP2015125084 A5 JP 2015125084A5 JP 2013270762 A JP2013270762 A JP 2013270762A JP 2013270762 A JP2013270762 A JP 2013270762A JP 2015125084 A5 JP2015125084 A5 JP 2015125084A5
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- reflective film
- light
- wavelength
- interference filter
- observation apparatus
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- 230000005284 excitation Effects 0.000 claims description 3
- 230000003287 optical Effects 0.000 claims 3
Description
本発明の一態様の蛍光観察装置は、励起光を含む波長の光を出射する光源部と、第１波長可変干渉フィルターと、観察部と、を含み、前記第１波長可変干渉フィルターは、第１反射膜と、前記第１反射膜に対向する第２反射膜と、前記第１反射膜と前記第２反射膜との間隔を変更可能なギャップ変更部と、を含み、前記光源部からの光に対し、前記第１反射膜は所定の角度で傾斜しており、前記光源部からの光のうちの所定波長以外の光を第１方向に出射させ、前記第１方向とは逆の方向である第２方向に前記第１反射膜に入射した光のうちの所定波長の光を前記第２方向に出射させ、前記観察部は、前記第１波長可変フィルターから前記第２方向に出射された光を観察し、前記所定波長の光は、前記間隔に対応していることを特徴とする。
上記の本発明に係る蛍光観察装置は、励起光を含む波長の光を出射する光源部と、互いに対向する一対の反射膜、及び、前記一対の反射膜のギャップ寸法を変更可能なギャップ変更部を有し、前記光源部の光出射方向に対して前記一対の反射膜が所定の角度で傾斜して配置され、前記光源部からの光のうち前記ギャップ寸法に応じた所定波長以外の光を第一方向に出射させ、前記第一方向から入射された光のうち前記ギャップ寸法に応じた前記所定波長の光を第二方向に出射させる第一波長可変干渉フィルターと、前記第一波長可変干渉フィルターから前記第二方向に出射された光を観察する観察部と、を具備していることを特徴とする。
A fluorescence observation apparatus according to an aspect of the present invention includes a light source unit that emits light having a wavelength including excitation light, a first wavelength variable interference filter, and an observation unit, and the first wavelength variable interference filter includes: Including a first reflective film, a second reflective film facing the first reflective film, and a gap changing unit capable of changing an interval between the first reflective film and the second reflective film, from the light source unit The first reflection film is inclined at a predetermined angle with respect to light, and emits light having a wavelength other than the predetermined wavelength out of the light from the light source unit in a first direction, which is opposite to the first direction. The light having a predetermined wavelength out of the light incident on the first reflection film in the second direction is emitted in the second direction, and the observation unit is emitted from the first wavelength tunable filter in the second direction. The light having the predetermined wavelength corresponds to the interval.
The fluorescence observation apparatus according to the present invention includes a light source unit that emits light having a wavelength including excitation light, a pair of reflective films that face each other, and a gap changing unit that can change a gap size of the pair of reflective films. And the pair of reflective films are disposed at a predetermined angle with respect to the light emission direction of the light source unit, and the light from the light source unit has a wavelength other than the predetermined wavelength according to the gap size. A first wavelength tunable interference filter that emits light of the predetermined wavelength according to the gap size among light incident in the first direction and emitted in the second direction; and the first wavelength variable interference. An observation unit for observing light emitted from the filter in the second direction.
Claims (6)
第１波長可変干渉フィルターと、
観察部と、
を含み、
前記第１波長可変干渉フィルターは、
第１反射膜と、
前記第１反射膜に対向する第２反射膜と、
前記第１反射膜と前記第２反射膜との間隔を変更可能なギャップ変更部と、
を含み、
前記光源部からの光に対し、前記第１反射膜は所定の角度で傾斜しており、
前記光源部からの光のうちの所定波長以外の光を第１方向に出射させ、
前記第１方向とは逆の方向である第２方向に前記第１反射膜に入射した光のうちの所定波長の光を前記第２方向に出射させ、
前記観察部は、前記第１波長可変フィルターから前記第２方向に出射された光を観察し、
前記所定波長の光は、前記間隔に対応していることを特徴とする蛍光観察装置。 A light source unit that emits light having a wavelength including excitation light;
A first tunable interference filter;
An observation section;
Including
The first wavelength variable interference filter is:
A first reflective film;
A second reflective film facing the first reflective film;
A gap changing unit capable of changing an interval between the first reflective film and the second reflective film;
Including
The first reflective film is inclined at a predetermined angle with respect to the light from the light source unit,
Emitting light having a wavelength other than the predetermined wavelength from the light source unit in the first direction;
Light having a predetermined wavelength out of light incident on the first reflective film in a second direction that is opposite to the first direction is emitted in the second direction;
The observation unit observes light emitted from the first wavelength tunable filter in the second direction,
The fluorescence observation apparatus , wherein the light having the predetermined wavelength corresponds to the interval .
前記光源部と前記第１波長可変干渉フィルターとの間に配置される第２波長可変干渉フィルターをさらに含み、
前記第２波長可変干渉フィルターは、
第３反射膜と、
前記第３反射膜に対向する第４反射膜と、
前記第３反射膜と前記第４反射膜との間隔を変更可能なギャップ変更部と、
を含むことを特徴とする蛍光観察装置。 The fluorescence observation apparatus according to claim 1,
Further comprising a second variable wavelength interference filter disposed between the light source unit first variable wavelength interference filter,
The second wavelength variable interference filter is:
A third reflective film;
A fourth reflective film facing the third reflective film;
A gap changing unit capable of changing an interval between the third reflective film and the fourth reflective film;
Fluorescence observation apparatus characterized by comprising a.
前記第１波長可変干渉フィルターと前記観察部との間に配置される第３波長可変干渉フィルターをさらに含み、
前記第３波長可変干渉フィルターは、
第５反射膜と、
前記第５反射膜に対向する第６反射膜と、
前記第５反射膜と前記第６反射膜との間隔を変更可能なギャップ変更部と、
を含むことを特徴とする蛍光観察装置。 In the fluorescence observation apparatus according to claim 1 or 2,
Further comprising a third variable wavelength interference filter disposed between said first variable wavelength interference filter and the observation unit,
The third wavelength variable interference filter is:
A fifth reflective film;
A sixth reflective film facing the fifth reflective film;
A gap changing unit capable of changing an interval between the fifth reflective film and the sixth reflective film;
Fluorescence observation apparatus characterized by comprising a.
前記光源部と第１波長可変干渉フィルターとの間に配置され、光の偏光方向を直線偏光にする第１偏光光学素子をさらに含むことを特徴とする蛍光観察装置。 In the fluorescence observation apparatus according to any one of claims 1 to 3,
The light source unit and disposed between the first variable wavelength interference filter, the fluorescence observation apparatus characterized by further comprising a first polarizing optical element for the polarization direction of the light into linearly polarized light.
前記第１波長可変干渉フィルターの前記第１方向の側に配置され、光の偏光方向を直線偏光にする第２偏光光学素子をさらに含むことを特徴とする蛍光観察装置。 In the fluorescence observation apparatus according to any one of claims 1 to 4,
The first is disposed on the side of the first direction of the variable wavelength interference filter, the fluorescence observation apparatus characterized by further comprising a second polarizing optical element for the polarization direction of the light into linearly polarized light.
前記第１反射膜に対向する第２反射膜と、
前記第１反射膜と前記第２反射膜との間隔を変更可能なギャップ変更部と、
を含み、
光源部からの光に対し、前記第１反射膜は所定の角度で傾斜しており、
前記光源部からの光のうちの所定波長以外の光を第１方向に出射させ、
前記第１方向とは逆の方向である第２方向に前記第１反射膜に入射した光のうちの所定波長の光を前記第２方向に出射させ、
前記所定波長の光は、前記間隔に対応していることを特徴とする光学部材。 A first reflective film;
A second reflective film facing the first reflective film;
A gap changing unit capable of changing an interval between the first reflective film and the second reflective film;
Including
The first reflective film is inclined at a predetermined angle with respect to light from the light source unit,
Emitting light having a wavelength other than the predetermined wavelength from the light source unit in the first direction;
Light having a predetermined wavelength out of light incident on the first reflective film in a second direction that is opposite to the first direction is emitted in the second direction;
The optical member , wherein the light having the predetermined wavelength corresponds to the interval .
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2013270762A JP6323004B2 (en) | 2013-12-27 | 2013-12-27 | Fluorescence observation apparatus and optical member |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2013270762A JP6323004B2 (en) | 2013-12-27 | 2013-12-27 | Fluorescence observation apparatus and optical member |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015125084A JP2015125084A (en) | 2015-07-06 |
| JP2015125084A5 true JP2015125084A5 (en) | 2017-01-19 |
| JP6323004B2 JP6323004B2 (en) | 2018-05-16 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013270762A Active JP6323004B2 (en) | 2013-12-27 | 2013-12-27 | Fluorescence observation apparatus and optical member |
Country Status (1)
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| JP (1) | JP6323004B2 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10724958B2 (en) * | 2017-11-06 | 2020-07-28 | Senors Unlimited, Inc. | Imaging devices, imaging arrangements, and imaging methods |
| JP2019148540A (en) * | 2018-02-28 | 2019-09-05 | セイコーエプソン株式会社 | Light measurement device and electronic apparatus |
| KR102380565B1 (en) * | 2020-09-18 | 2022-03-30 | (주)얼라인드제네틱스 | Sample inspection device for detecting fluorescence in the sample |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5852498A (en) * | 1997-04-04 | 1998-12-22 | Kairos Scientific Inc. | Optical instrument having a variable optical filter |
| US8395770B1 (en) * | 2004-10-05 | 2013-03-12 | Photon Systems | Spectroscopic chemical analysis methods and apparatus |
| US7764361B2 (en) * | 2006-07-27 | 2010-07-27 | Northwestern University | Systems and methods to analyze multiplexed bead-based assays using backscattered light |
| JP4669995B2 (en) * | 2006-08-02 | 2011-04-13 | ナノフォトン株式会社 | Optical microscope and observation method |
| JP2010197173A (en) * | 2009-02-24 | 2010-09-09 | Sumitomo Electric Ind Ltd | Foreign substance identification method |
| JP4979777B2 (en) * | 2010-01-18 | 2012-07-18 | オリンパス株式会社 | Microscope image processing device |
| JP6010275B2 (en) * | 2010-03-15 | 2016-10-19 | セイコーエプソン株式会社 | Optical filter and analytical instrument and optical instrument using the same |
| US10564412B2 (en) * | 2010-12-10 | 2020-02-18 | Nkt Photonics A/S | Tunable filter including an angular dispersive element for a broad band source |
| JP5900515B2 (en) * | 2012-01-18 | 2016-04-06 | 株式会社ニコン | Structured illumination device, structured illumination microscope device, structured illumination method |
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