JP2015093841A - Ethylene biosynthesis inhibitor for plant, and use thereof - Google Patents
Ethylene biosynthesis inhibitor for plant, and use thereof Download PDFInfo
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本発明は植物のエチレン生合成阻害剤とその利用に関し、詳しくは、２−アミノオキシイソ酪酸（ＡＯＩＢ）又はその塩を有効成分として含む植物のエチレン生合成阻害剤とその利用、特に、切り花を含む種々の植物の鮮度保持剤に関する。 The present invention relates to an ethylene biosynthesis inhibitor for plants and uses thereof, and more particularly, to an ethylene biosynthesis inhibitor for plants containing 2-aminooxyisobutyric acid (AOIB) or a salt thereof as an active ingredient and use thereof, particularly cut flowers. The present invention relates to a freshness-preserving agent for various plants.
エチレンは植物ホルモンの１種であって、常温常圧下では気体であり、脱離、老化、開花、実止り、果実登熟、種子発芽、性表現、根成長、節間伸長、上偏成長及び屈地性等の植物の成熟と老化に大きく関与するほか、多くの生理作用を有することが知られている。このようなエチレンは、植物体内において、酵素による触媒反応によって、メチオニンからＳ−アデノシル−Ｌ−メチオニン及び１−アミノシクロプロパン−１−カルボン酸（ＡＣＣ）を経て生合成される（特許文献１参照）。 Ethylene is a kind of plant hormone, which is a gas under normal temperature and normal pressure. Desorption, senescence, flowering, fruit set, fruit ripening, seed germination, sex expression, root growth, internode elongation, upside growth and In addition to being greatly involved in plant maturation and aging such as terrestrial, it is known to have many physiological effects. Such ethylene is biosynthesized from methionine through S-adenosyl-L-methionine and 1-aminocyclopropane-1-carboxylic acid (ACC) in plants by an enzyme-catalyzed reaction (see Patent Document 1). ).
より詳しくは、Ｓ−アデノシル−Ｌ−メチオニンからＡＣＣへの反応は１−アミノシクロプロパン−１−カルボン酸合成酵素（ＡＣＣ合成酵素）による触媒作用を受け、ＡＣＣからエチレンへの反応は１−アミノシクロプロパン−１−カルボン酸酸化酵素（ＡＣＣ酸化酵素）による触媒作用を受ける（非特許文献１参照）。 More specifically, the reaction from S-adenosyl-L-methionine to ACC is catalyzed by 1-aminocyclopropane-1-carboxylic acid synthase (ACC synthase), and the reaction from ACC to ethylene is 1-amino. It is catalyzed by cyclopropane-1-carboxylate oxidase (ACC oxidase) (see Non-Patent Document 1).
従って、上述したような植物体内におけるエチレンの生合成を抑制することによって、植物の成熟と老化を抑制して、果実、野菜、切り花等の鮮度保持を図ることができ、そこで、このような観点から、植物に作用させて、植物体内におけるエチレンの生合成を阻害する薬剤が、これまで、種々、開発されている。 Therefore, by suppressing the biosynthesis of ethylene in the plant body as described above, it is possible to suppress the maturation and aging of the plant and maintain the freshness of fruits, vegetables, cut flowers, etc. Therefore, various agents have been developed so far that act on plants to inhibit the biosynthesis of ethylene in the plants.
そのような薬剤は、切り花、葉物、鉢植え植物等を始めとする植物の鮮度保持や延命を目的として、通常、適宜の濃度の水溶剤として、生け水に用いたり、噴霧したり、或いは薬剤が気体である場合には、気浴処理したりして、それら植物に施用し、その老化防止や、また、品質の改善や維持が図られている。 Such drugs are usually used in fresh water, sprayed, or used as a water solvent with an appropriate concentration for the purpose of maintaining the freshness or extending the life of plants such as cut flowers, leaves, potted plants, etc. In the case where the gas is a gas, it is applied to those plants by air bath treatment, and the aging is prevented, and the quality is improved and maintained.
なかでも、ＳＴＳとして知られるチオ硫酸銀錯塩は、植物体内におけるエチレン受容体とエチレンが結合することによって開始するエチレンによる植物の成熟と老化の作用を抑えて、例えば、カーネーション、宿根カスミソウ等のエチレンが花の萎凋の主要因である切り花に対して、鮮度を保持する効果を有することがよく知られている。 Among them, a silver thiosulfate complex known as STS suppresses the effects of maturation and aging of the plant initiated by the binding of ethylene receptor and ethylene in the plant body. For example, ethylene such as carnation and perennial gypsophila Is well known to have the effect of maintaining freshness against cut flowers, which are the main cause of flower wilt.
しかし、ＳＴＳは、その有効成分が重金属である銀であることから、環境への負荷軽減の観点から、重金属を含まないエチレン生合成阻害剤が要望されており、既に、幾つかが提案されている。例えば、アミノオキシ酢酸（ＡＯＡ）はＡＣＣ合成酵素の触媒作用を阻害して、エチレン生合成を阻害し、また、２−アミノイソ酪酸（ＡＩＢ）はＡＣＣ酸化酵素の触媒作用を阻害して、エチレン生合成を阻害することから、これらは、切り花の鮮度保持剤として有効であることが既に知られている（特許文献２及び非特許文献１参照）。
However, since the active ingredient of STS is silver, which is a heavy metal, an ethylene biosynthesis inhibitor that does not contain heavy metals has been demanded from the viewpoint of reducing the burden on the environment, and some have already been proposed. Yes. For example, aminooxyacetic acid (AOA) inhibits the catalytic action of ACC synthase to inhibit ethylene biosynthesis, and 2-aminoisobutyric acid (AIB) inhibits the catalytic action of ACC oxidase to produce ethylene biosynthesis. Since they inhibit synthesis, they are already known to be effective as freshness-preserving agents for cut flowers (see
本発明者らは、ＳＴＳに代わる新たなエチレン生合成阻害剤を得るために、鋭意、研究した結果、ＡＯＩＢがＡＣＣ合成酵素とＡＣＣ酸化酵素の触媒活性を阻害することを見出し、更に、ＡＯＩＢがＡＣＣ合成酵素とＡＣＣ酸化酵素の触媒活性を阻害するこのような効果を有する結果、カーネーションを含む種々の切り花におけるエチレン生合成を阻害して、日持ち性を延長することを見出し、また、ＡＯＩＢがトルコギキョウの切り花の花の萎れの開始を抑制し、日持ち性を延長することを見出して、本発明に至ったものである。 As a result of earnest and research to obtain a new ethylene biosynthesis inhibitor that replaces STS, the present inventors have found that AOIB inhibits the catalytic activity of ACC synthase and ACC oxidase, and further, AOIB As a result of having such an effect of inhibiting the catalytic activity of ACC synthase and ACC oxidase, it has been found that ethylene biosynthesis in various cut flowers including carnation is inhibited, thereby extending the shelf life. AOIB is also found in Eustoma grandiflorum. The present invention has been found by suppressing the onset of wilting of cut flowers and prolonging shelf life.
従って、本発明は、ＳＴＳとして知られるチオ硫酸銀錯塩に代わる、植物のための新規なエチレン生合成阻害剤とその利用、詳しくは、花卉類やその切り花を始めとする種々の園芸観賞用植物の鮮度保持剤を提供することを目的とする。 Accordingly, the present invention relates to a novel ethylene biosynthesis inhibitor for plants, which is an alternative to silver thiosulfate complex known as STS, and uses thereof, and in particular, various horticultural ornamental plants including flower buds and their cut flowers. An object of the present invention is to provide a freshness-preserving agent.
本発明によれば、ＡＯＩＢ又はその塩を有効成分とする植物のエチレン生合成阻害剤が提供される。 ADVANTAGE OF THE INVENTION According to this invention, the ethylene biosynthesis inhibitor of the plant which uses AOIB or its salt as an active ingredient is provided.
更に、本発明によれば、ＡＯＩＢ又はその塩を有効成分とする植物の鮮度保持剤が提供される。 Furthermore, according to this invention, the freshness preservation agent of the plant which uses AOIB or its salt as an active ingredient is provided.
特に、本発明において、上記植物は、好ましくは、園芸観賞用植物であり、園芸観賞用植物は、好ましくは、花卉類であり、花卉類は、好ましくは、切り花である。 In particular, in the present invention, the plant is preferably a horticultural ornamental plant, the horticultural ornamental plant is preferably a floret, and the floret is preferably a cut flower.
本発明による植物のエチレン生合成阻害剤はＡＯＩＢ又はその塩を有効成分とし、特に、園芸観賞用植物、なかでも、カーネーションやトルコギキョウの切り花の鮮度保持剤として効果にすぐれており、それら切り花の日持ち日数を大幅に増加することができる。 The plant ethylene biosynthesis inhibitor according to the present invention comprises AOIB or a salt thereof as an active ingredient, and is particularly effective as a freshness-preserving agent for horticultural ornamental plants, especially carnations and lisianthus cut flowers. The number of days can be greatly increased.
本発明によるエチレン生合成阻害剤はＡＯＩＢ又はその塩を有効成分とする。ＡＯＩＢは、既に知られている化学物質である。ＡＯＩＢの塩とは、農学的に許容される無機酸又は有機酸の塩であって、例えば、塩酸、硫酸、リン酸等の無機酸、酢酸、シュウ酸、マレイン酸、コハク酸、クエン酸等の有機酸を挙げることができる。 The ethylene biosynthesis inhibitor according to the present invention contains AOIB or a salt thereof as an active ingredient. AOIB is a known chemical substance. The salt of AOIB is an agriculturally acceptable salt of inorganic acid or organic acid, for example, inorganic acid such as hydrochloric acid, sulfuric acid, phosphoric acid, acetic acid, oxalic acid, maleic acid, succinic acid, citric acid, etc. The organic acid can be mentioned.
花卉類の切り花、例えば、カーネーションやトルコギキョウの切り花は、運搬輸送される間に種々のストレスを受けたり、外生エチレンに曝されたりすることによって、また、受粉することによって、植物の体内でエチレンが生合成され、その作用によって、それら切り花の老化が促進されて、観賞期間が著しく短縮される。本発明によるエチレン生合成阻害剤は、これを花卉類の切り花に施用することによって、切り花内におけるエチレンの生合成を阻害し、その結果、切り花の鮮度を保持することができる。 Cut flowers of flower buds, such as carnations and eustoma flowers, are subjected to various stresses during transportation and transportation, exposed to exogenous ethylene, and pollinated to produce ethylene in the plant body. Are biosynthesized, and their action promotes the senescence of these cut flowers, thereby significantly reducing the viewing period. The ethylene biosynthesis inhibitor according to the present invention is applied to cut flowers of flower buds to inhibit ethylene biosynthesis in the cut flowers, and as a result, the freshness of the cut flowers can be maintained.
本発明によるエチレン生合成阻害剤を鮮度保持剤として有効に施用することができる植物として、例えば、カーネーション、トルコギキョウ、デルフィニウム、スイートピー、宿根カスミソウ、ユリ、フリージア、チューリップ、バラ、キンギョソウ、ストック、スイセン、ハイブリッドスターチス又はラン類等の切り花や鉢物等を挙げることができるが、これらに限定されるものではない。 Examples of plants that can be effectively applied with the ethylene biosynthesis inhibitor according to the present invention as a freshness-preserving agent include carnation, eustoma, delphinium, sweet pea, perennial gypsophila, lily, freesia, tulip, rose, snapdragon, stock, narcissus, Examples include, but are not limited to, cut flowers and pots such as hybrid statics or orchids.
本発明によるエチレン生合成阻害剤は、通常、固体担体や液体担体等の不活性担体と混合し、必要に応じて、界面活性剤、その他の製剤用補助剤等を添加して、乳剤、水和剤、懸濁剤、水溶剤、粉末剤等に製剤化して用いられる。 The ethylene biosynthesis inhibitor according to the present invention is usually mixed with an inert carrier such as a solid carrier or a liquid carrier, and if necessary, a surfactant, other formulation adjuvants, etc. are added to the emulsion, It is used by formulating into a compatibilizer, suspending agent, aqueous solvent, powder, etc.
特に、本発明によるエチレン生合成阻害剤は、好ましくは、植物の鮮度保持剤として用いられる。 In particular, the ethylene biosynthesis inhibitor according to the present invention is preferably used as a plant freshness-preserving agent.
前述したように、植物はその個体内でエチレンを生合成し、その植物を成熟させ、また老化させるが、植物は、その個体から切り離された部位においても、エチレンを生合成し、その部位の成熟と老化をもたらす。例えば、切り花は花の個体の一部位であり、バナナ等の果物やニンジン等の根もまた植物の個体の一部位である。従って、本発明においては、植物とは、植物のそれぞれの個体のみならず、植物の花、葉、果実、根、球根、枝、茎等、それら植物の個体から分離された部位も含むものとする。 As described above, a plant biosynthesizes ethylene in its individual, matures and ages the plant, but the plant biosynthesizes ethylene even in a site separated from the individual, Brings maturity and aging. For example, a cut flower is a part of a flower individual, and a fruit such as a banana or a root of a carrot is also a part of a plant individual. Therefore, in the present invention, the term “plant” includes not only individual plants, but also plants, flowers, leaves, fruits, roots, bulbs, branches, stems, and other parts separated from these plants.
なかでも、本発明において、上記植物は、好ましくは、園芸観賞用植物であり、園芸観賞用植物は、好ましくは、花卉類であり、花卉類は、好ましくは、切り花である。 In particular, in the present invention, the plant is preferably a horticultural ornamental plant, the horticultural ornamental plant is preferably a floret, and the floret is preferably a cut flower.
ここに、本発明において、花卉類とは、切り花類、切り葉類、切り枝類、実（み）物類、花木類、鉢物類、花壇用苗物類及び球根類を含む園芸観賞用植物を意味するものとする。 Here, in the present invention, the florets are cut flowers, cut leaves, cut branches, berries, flowering trees, potted plants, flowerbed seedlings, and bulbous plants. Means.
本発明によるエチレン生合成阻害剤をこのような植物の鮮度保持剤として用いる場合には、通常、ＡＯＩＢ又はその塩を水に溶解させて、水溶剤とし、そのままで、又は更に水で希釈して、対象となる植物に施用される。植物への施用の方法としては、例えば、切り花の生け水に加えたり、切り花の生け水としたり、また、スプレーを用いて、切り花全体に散布したりすればよい。 When the ethylene biosynthesis inhibitor according to the present invention is used as a freshness-preserving agent for such a plant, usually, AOIB or a salt thereof is dissolved in water to form an aqueous solvent, as it is, or further diluted with water. Applied to the target plant. As a method for applying to a plant, for example, it may be added to the ikebana of cut flowers, used as ikebana of cut flowers, or sprayed over the whole cut flowers using a spray.
本発明によるエチレン生合成阻害剤を水溶剤からなる鮮度保持剤として、植物、例えば、花卉類に施用するに際しては、一般には、０．０１〜１００ｍＭの範囲の濃度、好ましくは、０．０５〜５０ｍＭの範囲の濃度、最も好ましくは、０．１〜１０ｍＭの範囲の濃度で施用される。 When applying the ethylene biosynthesis inhibitor according to the present invention to a plant such as a floret as a freshness-preserving agent comprising an aqueous solvent, the concentration is generally in the range of 0.01 to 100 mM, preferably 0.05 to It is applied at a concentration in the range of 50 mM, most preferably at a concentration in the range of 0.1-10 mM.
本発明によるこのような植物の鮮度保持剤は、従来、鮮度保持剤として知られている殺菌剤、糖類、植物成長調整剤、植物ホルモン等と混合して用いてもよい。また、必要に応じて、界面活性剤を配合してもよい。 Such a plant freshness-keeping agent according to the present invention may be used by mixing with a bactericide, saccharide, plant growth regulator, plant hormone and the like conventionally known as freshness-keeping agents. Moreover, you may mix | blend surfactant as needed.
以下に実施例を挙げて本発明を説明するが、本発明はこれら実施例によって何ら限定されるものではない。 EXAMPLES The present invention will be described below with reference to examples, but the present invention is not limited to these examples.
実施例１
（ＡＯＩＢのＡＣＣ合成酵素及びＡＣＣ酸化酵素阻害作用）
ＡＣＣ合成酵素及びＡＣＣ酸化酵素は、カーネーションのＡＣＣ合成酵素遺伝子（ＤｃＡＣＳ１） とＡＣＣ酸化酵素遺伝子（ＤｃＡＣＯ１）を大腸菌を用いてアミノ末端に６個の連続するヒスチジン（Ｈｉｓ）残基からなるペプチド（Ｈｉｓ−タグ)を結合した酵素タンパク質として発現させた。
Example 1
(AOIB inhibits ACC synthase and ACC oxidase)
ACC synthase and ACC oxidase are a peptide (His) consisting of six consecutive histidine (His) residues at the amino terminus of carnation ACC synthase gene (DcACS1) and ACC oxidase gene (DcACO1) using Escherichia coli. -Tag) was expressed as a bound enzyme protein.
このタンパク質について、ニッケルイオンを固定化した担体を用いたカラムクロマトグラフィーを行って、Ｈｉｓ−タグのヒスチジン残基とニッケルイオンのキレート作用によりタンパク質を担体に結合させた。緩衝液で担体を洗浄して、混在する不純タンパク質を除去した後、イミダゾールを添加した緩衝液で溶出して、結合しているＨｉｓ−タグを持つタンパク質を担体から回収し、Ｈｉｓ−タグＡＣＣ合成酵素タンパク質又はＨｉｓ−タグＡＣＣ酸化酵素タンパク質として使用した。 This protein was subjected to column chromatography using a carrier on which nickel ions were immobilized, and the protein was bound to the carrier by the chelating action of the histidine residue of the His-tag and nickel ions. After washing the carrier with a buffer solution to remove mixed proteins, the protein with bound His-tag is recovered from the carrier by elution with a buffer solution to which imidazole is added, and His-tag ACC synthesis is performed. Used as enzyme protein or His-tagged ACC oxidase protein.
ＡＣＣ合成酵素活性は、１５０μＬの酵素液を１００ｍＭ濃度の４−（２−ヒドロキシエチル）−１−ピペラジンエタンスルホン酸（ＨＥＰＥＳ、ＫＯＨにてｐＨ８．５に調整したもの）、５μＭ濃度のピリドキサールリン酸（ＰＬＰ）、１００μＭ濃度のＳ−アデノシル−Ｌ−メチオニン及び所定濃度の阻害剤と共に、総容量４００μＬにしてインキュベートし、測定した。３０℃で１５分間、インキュベーションした後、Ｌｉｚａｄａ ａｎｄ Ｙａｎｇの方法でＡＣＣ生成量を測定した。 The ACC synthase activity was determined by using 150 μL of the enzyme solution at a concentration of 100 mM 4- (2-hydroxyethyl) -1-piperazineethanesulfonic acid (pHEP 8.5 adjusted with HEPES, KOH), and 5 μM pyridoxal phosphate. (PLP), 100 μM concentration of S-adenosyl-L-methionine and a predetermined concentration of inhibitor were incubated at a total volume of 400 μL and measured. After incubation at 30 ° C. for 15 minutes, the amount of ACC produced was measured by the method of Lizada and Yang.
ＡＣＣ酸化酵素は、５０μＬの酵素液を６０ｍＭ濃度の（Ｎ−モルホリノ）プロパンスルホン酸（ＭＯＰＳ、ＮａＯＨにてｐＨ７．５に調整したもの）、２５ｍＭ濃度のアスコルビン酸ナトリウム、５０μＭ濃度のＦｅＳＯ4、２５ｍＭ濃度のＮａＨＣＯ3、０．１ｍＭ濃度のＡＣＣ及び所定濃度の阻害剤と共に総容量１ｍＬにして、インキュベートし、測定した。 As for ACC oxidase, 50 μL of enzyme solution (N-morpholino) propanesulfonic acid (MOPS, adjusted to pH 7.5 with NaOH), 25 mM sodium ascorbate, 50 μM FeSO 4 , 25 mM A total volume of 1 mL with a concentration of NaHCO 3 , 0.1 mM ACC and a given concentration of inhibitor was incubated and measured.
酵素反応は５ｍＬのガラス試験管にゴム栓で蓋をして行った。３０℃で１５分間、インキュベーションした後、ヘッドスペースから１ｍＬの気体を採取し、ガスクロマトグラフィーでエチレン分析を行った。結果を表１及び表２に示す。 The enzyme reaction was carried out by covering a 5 mL glass test tube with a rubber stopper. After incubation at 30 ° C. for 15 minutes, 1 mL of gas was collected from the headspace, and ethylene analysis was performed by gas chromatography. The results are shown in Tables 1 and 2.
表１及び表２から明らかなように、ＡＯＩＢはＡＯＡと同様にＡＣＣ合成酵素活性を阻害し、ＡＩＢと同様にＡＣＣ酸化酵素活性を阻害した。 As is clear from Tables 1 and 2, AOIB inhibited ACC synthase activity like AOA, and inhibited ACC oxidase activity like AIB.
実施例２
（ＡＯＩＢのエチレン生成抑制効果）
市場出荷に適応した出荷ステージで採花されたカーネーション切り花（品種エクセリア）を乾式で輸送した後、茎元を切り戻して、水に生け、外側の花弁が茎に対して垂直になるまで開花させて、エチレン生成測定用の供試花とした。
Example 2
(AOIB suppresses ethylene production)
Carnation cut flowers (varietal exceria) harvested at the shipping stage adapted to market shipment are transported dry, then cut back at the stem, placed in water, and flowered until the outer petals are perpendicular to the stem A sample flower for measurement of ethylene production was used.
供試花を５ｃｍの長さに切り、１５ｍＬの各処理液が入った５０ｍＬのガラス瓶に１本ずつ生けた。処理液は１日おきに交換した。エチレン生成量は、供試花を６００ｍＬガラス容器に入れ、２５℃で１時間保持した後、ガラス容器中のヘッドスペースから１ｍＬの気体を採取して、ガスクロマトグラフィーで測定した。結果を図１に示す。 The test flowers were cut into 5 cm lengths and placed one by one in a 50 mL glass bottle containing 15 mL of each treatment solution. The processing solution was changed every other day. The amount of ethylene produced was measured by gas chromatography after putting the sample flower in a 600 mL glass container and holding it at 25 ° C. for 1 hour, then collecting 1 mL of gas from the head space in the glass container. The results are shown in FIG.
図１（ｅ）及び（ｆ）から明らかなように、ＡＯＩＢはエチレン生成を遅延して、その生成量を抑制することが認められた。特に、１．０ｍＭ濃度では、ほぼ完全にエチレン生成を抑制した。このＡＯＩＢのエチレン生成抑制の効果は、図１（ｂ）及び（ｃ）に示すように、ＡＯＡとほぼ同等であった。一方、図１（ｄ）に示すように、ＡＩＢは１０ｍＭ濃度もの高濃度においても多量のエチレンの生成が認められ、ＡＯＩＢはＡＩＢよりはるかに低濃度でエチレン生合成を抑制した。 As is apparent from FIGS. 1 (e) and (f), it was recognized that AOIB delays ethylene production and suppresses its production. In particular, at a concentration of 1.0 mM, ethylene production was almost completely suppressed. As shown in FIGS. 1 (b) and 1 (c), the effect of suppressing the production of ethylene by AOIB was almost the same as that of AOA. On the other hand, as shown in FIG. 1 (d), AIB produced a large amount of ethylene even at a concentration as high as 10 mM, and AOIB suppressed ethylene biosynthesis at a much lower concentration than AIB.
実施例３
（カーネーションの切り花におけるＡＯＩＢの鮮度保持効果（その１））
開花したカーネーション（品種エクセリア）を３０ｃｍの長さに切り、３０ｍＬの処理液の入ったガラス瓶に１本ずつ生け、２３℃の恒温室に置いて、切り花の状態を観察した。処理液は毎日調製し、供試花を生け替えた。試験区ごとに３本の花について老化の評価を行って、下記の４段階で評価した。
Example 3
(AOIB freshness retention effect on cut carnation flowers (Part 1))
The flowered carnation (variety exceria) was cut into 30 cm lengths, placed one by one in a glass bottle containing 30 mL of the treatment solution, and placed in a thermostatic chamber at 23 ° C., and the state of the cut flowers was observed. The treatment solution was prepared every day and the test flowers were replaced. Aging was evaluated for three flowers in each test section, and the following four levels were evaluated.
１ 完全開花の最終段階である。
２ 花弁のインロールが見られ、萎れが始まる。
３ 花弁全体が萎れる。
４ 花弁が著しく萎れて、収縮する。
1 It is the final stage of complete flowering.
2 Petal in-rolls are seen and wilting begins.
3 The whole petal wilts.
4 The petals are extremely deflated and contract.
図２に示す結果から明らかなように、ＡＯＩＢは、特に、１ｍＭ濃度において、カーネーションの切り花の萎凋を抑制して、高い鮮度保持効果を有することが認められ、想定される適用濃度でのその効果はＡＩＢよりはるかに大きく、ＡＯＡとほぼ同等であった。 As is clear from the results shown in FIG. 2, AOIB was found to have a high freshness-keeping effect by suppressing the wilt of carnation cut flowers, particularly at 1 mM concentration, and its effect at the assumed application concentration. Was much larger than AIB and almost equivalent to AOA.
実施例４
（カーネーションの切り花におけるＡＯＩＢの鮮度保持効果（その２））
採花されたカーネーション（品種モモカ及びチェリーテッシノ）を表３に示す各処理剤で２１．５時間処理した後、生け水と茎元の腐敗を防ぐために、対照区（水）を含む全試験区において、抗菌剤を溶解させた水溶液に生け替え、２５℃の恒温室に置いて、その状態を観察した。
Example 4
(AOIB freshness retention effect on cut carnation flowers (Part 2))
After treating the carnations (variety Momoka and Cherry Tessino) that were harvested for 21.5 hours with each treatment agent shown in Table 3, all test groups including the control group (water) were used to prevent the decay of raw water and stems. Then, it was replaced with an aqueous solution in which an antibacterial agent was dissolved and placed in a thermostatic chamber at 25 ° C., and the state was observed.
花弁の５０％以上が萎凋した時点を寿命とし、それまでの日数を日持ち日数とした。また、各試験区の日持ち日数と対照区の日持ち日数の差を日持ち延長日数として、併せて示した。 The time when 50% or more of the petals wilted was regarded as the lifespan, and the number of days until that was regarded as the number of days remaining. In addition, the difference between the number of days in each test zone and the number of days in the control zone was also shown as the number of days extended.
表３から明らかなように、ＡＯＩＢの鮮度保持効果は、低濃度において、ＡＩＢよりも高く、０．３０〜１０ｍＭ濃度の範囲において、ＡＯＡとほぼ同等であった。 As is clear from Table 3, the freshness-keeping effect of AOIB was higher than that of AIB at a low concentration, and almost equivalent to that of AOA in the range of 0.30 to 10 mM.
実施例５
（トルコギキョウの切り花におけるＡＯＩＢの鮮度保持効果）
トルコギキョウ（品種ニューリネーションピンク）を第一花が開花した当日に収穫し、表４に示す各処理液で２４時間処理した。処理後、水道水に生け替え、２５℃の恒温室に置いて、正常に発達しているつぼみ、開花及び萎凋の割合を観察した。
Example 5
(AOIB freshness preservation effect in cut flowers of Eustoma grandiflorum)
Eustoma grandiflorum (variety Newlination Pink) was harvested on the day when the first flower bloomed and treated with each treatment solution shown in Table 4 for 24 hours. After the treatment, it was replaced with tap water and placed in a constant temperature room at 25 ° C., and the proportions of buds, flowering and wilting that were normally developed were observed.
表４から明らかなように、ＡＯＩＢの萎凋の抑制効果は、低濃度でも、その１０倍の高濃度のＡＩＢよりもはるかに高く、ＡＯＡと比較しても、少なくとも同等以上の効果が認められた。
As is clear from Table 4, the effect of AWIB on the suppression of wilting was much higher than that of AIB having a
Claims (6)
The freshness-preserving agent according to claim 5, wherein the cut flowers are carnation, eustoma, delphinium, sweet pea, perennial gypsophila, lily, freesia, tulip, rose, snapdragon, stock, narcissus, hybrid starch or orchid.
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| JP2013232624A JP2015093841A (en) | 2013-11-09 | 2013-11-09 | Ethylene biosynthesis inhibitor for plant, and use thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105993558A (en) * | 2016-06-27 | 2016-10-12 | 安徽梅兰园林景观工程有限公司 | Cutting propagation technology for Gypsophila paniculata |
| CN110731220A (en) * | 2018-07-20 | 2020-01-31 | 北京市门头沟区科技开发实验基地 | Cultivation method for delaying flowering of landscape roses |
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2013
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105993558A (en) * | 2016-06-27 | 2016-10-12 | 安徽梅兰园林景观工程有限公司 | Cutting propagation technology for Gypsophila paniculata |
| CN110731220A (en) * | 2018-07-20 | 2020-01-31 | 北京市门头沟区科技开发实验基地 | Cultivation method for delaying flowering of landscape roses |
| CN110731220B (en) * | 2018-07-20 | 2021-09-17 | 北京市门头沟区科技开发实验基地 | Cultivation method for delaying blooming of landscape roses |
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