JP2015089355A - Container for microorganism culture sheet, and method of using the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To add sample solution to a microorganism culture sheet in a state where the microorganism culture sheet is housed in a container for the microorganism culture sheet.SOLUTION: A container 30 for a microorganism culture sheet comprises a box 30A having a bottom surface 31 and four side surfaces 32. At least one side surface 32a of the box 30A rotates with respect to the bottom surface 31 via a rotary part 33a.

Description

  The present invention relates to a microorganism culture sheet container and a method for using the same, and more particularly, to a microorganism culture sheet container and a method for using the same in which a sample solution can be easily added.

  Many microorganism culture sheets for measuring microorganisms in food or the environment have been devised. A typical microorganism culture sheet includes a rectangular waterproof sheet, a frame formed thereon, a medium layer formed inside the frame, and a cover sheet covering the medium layer. In recent years, the microorganism culture sheet has been widely used as an alternative to the agar medium because it has advantages such as less time for medium adjustment, less space, and reduced disposal costs compared to the conventional agar medium.

  For example, a cold water-soluble powder containing a water-based adhesive composition layer and a gelling agent attached to the adhesive composition layer is formed on the surface of the waterproof sheet, and the cold water-soluble powder is covered with the cover sheet. A microorganism culture sheet is obtained by covering with. In such a microorganism culture sheet, the cover sheet is peeled off, and an aqueous test sample is added on the cold water-soluble powder layer. In this case, the gelling agent quickly hydrates and forms a reconstitution medium that maintains microbial growth. Next, when the cover sheet is covered and incubated for a predetermined time, colonies can be counted through the transparent cover sheet (Patent Document 1).

  There is also a microorganism culture sheet in which a circular porous matrix layer and a water-soluble polymer compound layer are laminated on a square pressure-sensitive adhesive sheet, and a square transparent film is disposed on the top. When the sample solution is added onto the porous matrix layer, the sample solution is retained in the porous matrix layer, and the retained water dissolves the water-soluble polymer compound layer in contact with the porous matrix layer, resulting in water solubility. It becomes an environment where microorganisms grow by the polymer compound aqueous solution and the growth nutrient component. In this microorganism culture sheet, since the microorganisms do not penetrate into the water-soluble polymer compound layer and are further pushed up to the surface of the porous matrix layer, colonies are formed on or near the surface of the porous matrix layer, and the transparent film Can be used as a microorganism culture sheet by adhering to a pressure-sensitive adhesive sheet, and is lightweight, low in volume and easy to handle (Patent Document 2).

Japanese Patent Publication No. 3383304 International Publication No. W001 / 044437

  Since all the microorganism culture sheets are hydrated after a predetermined time has elapsed since the addition of the sample solution, when using a plurality of sheets, it is necessary to handle each sheet in a limited space. In addition, in the case of laminating microbial culture sheets that are hydrated during culture, the sample solution may leak if the microbial culture sheets are not sufficiently hydrated.

  The present invention has been made in consideration of such points, and a microorganism culture sheet container capable of reliably laminating a microorganism culture sheet and adding a sample solution to the microorganism culture sheet in a laminated state, and the same The purpose is to provide a method of use.

  The present invention is a microorganism culture sheet container for storing a plurality of microorganism culture sheets having a culture medium configured to culture microorganisms, and includes a box having a bottom surface and a plurality of side surfaces surrounding the bottom surface, At least one side surface is a container for a microorganism culture sheet, wherein the box body is opened by rotating with respect to the bottom surface through a rotating portion.

  In the present invention, the rotating side surface of the box may rotate within a range of 0 ° to 90 ° with respect to the bottom surface.

  In the present invention, the rotating side surface of the box may have one of a protruding portion and a receiving portion, and the other of the protruding portion and the receiving portion may be provided on a side surface adjacent to the rotating side surface.

  In the present invention, an opening may be formed in the microorganism culture sheet, and a support column inserted into the opening of the microorganism culture sheet may be provided on the bottom surface of the box.

  The present invention is a microorganism culture sheet container for storing a plurality of microorganism culture sheets having a medium configured to culture microorganisms, the container having a bottom surface and a plurality of side surfaces surrounding the bottom surface. In the method of using a container for a microorganism culture sheet, wherein at least one side surface is rotated with respect to the bottom surface via a rotating portion to open the box, the rotatable side surface is rotated with respect to the bottom surface. A method for using a container for a microorganism culture sheet, comprising: a step of opening the box body; and a step of adding a sample solution to a culture medium of the microorganism culture sheet in the opened box body.

  As described above, according to the present invention, a plurality of microorganism culture sheets can be reliably stacked, and the sample solution can be added to the microorganism culture sheet while the plurality of microorganism culture sheets are stacked.

FIG. 1 is a diagram showing a microorganism culture sheet before inoculating a sample solution containing microorganisms. FIG. 2 is a diagram showing a microorganism culture sheet in which a sample solution containing microorganisms is inoculated into the microorganism culture sheet of FIG. 1 to form colonies in a medium region. 3A, 3B, and 3C are views showing an embodiment of a container for a microorganism culture sheet. 4 (a), 4 (b), and 4 (c) are diagrams showing another embodiment of the microorganism culture sheet container. FIG. 5 is a view showing an engaging portion and a locking portion provided on the side surface of the microorganism culture sheet container.

Embodiment of the Invention

First Embodiment Hereinafter, a first embodiment of the present invention will be described with reference to the drawings.

  1 to 3 and FIG. 5 are views showing a first embodiment of the present invention.

(Microorganism culture sheet)
First, the microorganism culture sheet 20 accommodated in the microorganism culture sheet container will be described with reference to FIGS.

  The microorganism culture sheet 20 includes a rectangular waterproof sheet 11 and a cylindrical frame body 14 provided on the rectangular waterproof sheet 11, and a culture medium region 21 configured to form a colony 21 a in the frame body 14. (Also referred to as a medium) is formed, and a non-medium region (also referred to as a part other than the medium) 22 is formed outside the frame body 14. A cover sheet 13 having substantially the same shape as the rectangular waterproof sheet 11 is provided by being fixed at one end of the rectangular waterproof sheet 11.

  In FIG. 2, a microorganism culture sheet main body 20 </ b> A is configured by a rectangular waterproof sheet 11, a frame body 14 that partitions a culture medium region 21 and a non-medium culture region 22, and a gelled culture medium formed inside the frame body 14. The microorganism culture sheet 20 is constituted by the microorganism culture sheet main body 20A and the cover sheet 13.

  By the way, such a microorganism culture sheet 20 is used by the following methods. That is, first, a rectangular waterproof sheet 11, a frame 14 provided on the rectangular waterproof sheet 11, and a dry medium layer provided in the frame 14 and containing a gelling agent, a nutrient, a water absorbing agent, and the like. A microorganism culture sheet 20 before inoculation with a sample solution containing microorganisms having a medium region 21 (medium) and a cover sheet 13 is prepared (see FIG. 1). Next, after inoculating a sample solution containing microorganisms on the medium region 21 composed of the dry medium layer of the microorganism culture sheet 20 and gelling with a gelling agent, the microorganisms in the gelling medium are cultured under appropriate conditions. . As a result, colonies 21a are formed in the medium region 21 (see FIG. 2). The colony 21a is colored by the indicator in the gelling medium.

(Microbial culture sheet container)
Next, referring to FIG. 3 and FIG. 5, a microorganism culture sheet container 30 for storing a plurality of microorganism culture sheets 20 in a stacked manner will be described.

  As shown in FIGS. 3 and 5, the microorganism culture sheet container 30 includes a box-shaped box body 30 </ b> A having a rectangular bottom surface 31 and four side surfaces 32 a, 32 b, 32 c, and 32 d surrounding the bottom surface 31.

  Of these, the four side surfaces 32a, 32b, 32c, and 32d of the box-shaped box body 30A are all rotatable with respect to the bottom surface 31 via rotating portions (hinges) 33a, 33b, 33c, and 33d. ing. The box-shaped box 30A is made of synthetic resin as a whole.

  As described above, all of the four side surfaces 32a, 32b, 32c, and 32d are rotatable with respect to the bottom surface 31, and with respect to the bottom surface 31, from a horizontal position (0 ° position) to a vertical position (90 ° position). It can be rotated over a range up to (position).

  Further, an engaging portion (projection portion) 35 is provided on one end side of the upper edge of each side surface 32a, 32b, 32c, 32d, and a locking portion (receiving portion) 36 is provided on the other end side of the upper edge. Yes. When the side surfaces 32a, 32b, 32c, and 32d are all brought to the vertical position, for example, the engaging portion 35 of the side surface 32a engages with the engaging portion 36 of the adjacent side surface 32d, and the engaging portion 35 of the side surface 32b. Engages with the engaging portion 36 of the adjacent side surface 32a (see FIG. 5). Note that the engaging portion (projecting portion) 35 and the locking portion (receiving portion) 36 may be disposed opposite to each other.

  Next, the operation of the present embodiment having such a configuration will be described.

  First, a microorganism culture sheet container 30 is prepared (FIG. 3A). Next, the two side surfaces 32 a and 32 b of the microorganism culture sheet container 30 are brought down to a horizontal position with respect to the bottom surface 31. In this way, the two side surfaces 32a and 32b of the box body 30A of the microorganism culture sheet container 30 are opened.

  Next, as shown in FIG. 3 (b), the microorganism culture sheet 20 is inserted from the opened two side surfaces 32 a and 32 b in the microorganism culture sheet container 30, and the microorganism culture sheet 20 is inserted into the microorganism culture sheet container. 30.

  Next, a sample solution containing microorganisms is added (inoculated) to the culture medium 21 of the microorganism culture sheet 20 to cause gelation. By sequentially repeating such operations, a plurality of microorganism culture sheets 20 can be stacked and stored in the microorganism culture sheet container 30.

  In this case, the remaining side surfaces 32c and 32d of the microorganism culture sheet container 30 are kept vertical with respect to the bottom surface 31 without falling down. Therefore, the laminated microorganism culture sheet 20 can be held in the microorganism culture sheet container 30 while being positioned by the side surfaces 32c and 32d.

  In this way, the operation of adding the sample solution to the culture medium 21 of the microorganism culture sheet 20 is completed, and the side surfaces 32a and 32b are raised to the vertical position with respect to the bottom surface 31 (see FIG. 3C).

  Thereafter, the microorganism culture sheet container 30 in which the microorganism culture sheet 20 is accommodated is placed in an incubator as a whole and cultured for a predetermined time, whereby the microorganism culture sheet 20 in which the colonies 21a are formed in the medium 21 is obtained.

  As described above, according to the present embodiment, in the state where the two side surfaces 32 a and 32 b of the microorganism culture sheet container 30 are tilted and the microorganism culture sheet container 30 is opened, the microorganisms are contained in the microorganism culture sheet container 30. The culture sheet 20 can be inserted, and a sample solution can be added to the microorganism culture sheet 20. For this reason, it is possible to easily perform the operation of inserting the microorganism culture sheet 20 into the microorganism culture sheet container 30 and the operation of adding the sample solution. Further, the plurality of microorganism culture sheets 20 can be reliably stacked in the microorganism culture sheet container 30 while being positioned.

Second Embodiment Next, a second embodiment of the present invention will be described with reference to FIGS. 4 (a), 4 (b) and 4 (c).

  In the second embodiment shown in FIGS. 4 (a), (b), and (c), the microorganism culture sheet container 30 includes a box 30A and a column 37 that is erected on the bottom surface 31 of the box 30A. Each microbe culture sheet 20 is formed with an opening 25 into which a support 37 is inserted.

  Other configurations are substantially the same as those of the first embodiment shown in FIG. 1 to FIG. 3 and FIG.

  In the second embodiment shown in FIGS. 4 (a), 4 (b) and 4 (c), the same parts as those in the first embodiment shown in FIGS. 1 to 3 and FIG. Description is omitted.

  In the second embodiment having such a configuration, first, a microorganism culture sheet container 30 is prepared (FIG. 4A). Next, all four side surfaces 32 a, 32 b, 32 c, 32 d of the microorganism culture sheet container 30 are brought down to a horizontal position with respect to the bottom surface 31. In this way, all four side surfaces 32a, 32b, 32c, 32d of the box 30A of the microorganism culture sheet container 30 are opened.

  Next, as shown in FIG. 4 (b), the microorganism culture sheet 20 is inserted into the microorganism culture sheet container 30 from any one of the four side surfaces 32a, 32b, 32c, and 32d opened, and this microorganism The culture sheet 20 is stored in the microorganism culture sheet container 30. In this case, the support 37 is inserted into the opening 25 of the microorganism culture sheet 20, and the support 37 is inserted into the opening 25 to position the microorganism culture sheet 20 on the bottom surface 31.

  The microorganism culture sheet 20 may be inserted into the microorganism culture sheet container 30 from the upper side of the microorganism culture sheet container 30 (the portion opened when the state shown in FIG. 4C).

  Next, a sample solution containing microorganisms is added (inoculated) to the culture medium 21 of the microorganism culture sheet 20 to cause gelation. By sequentially repeating such operations, a plurality of microorganism culture sheets 20 can be stacked and stored in the microorganism culture sheet container 30.

  In this case, the support 37 provided on the bottom surface 31 of the microorganism culture sheet container 30 is inserted into the opening 25 of the microorganism culture sheet 20, and the microorganism culture sheet 20 stacked on the bottom surface 31 is positioned.

  In this way, the operation of adding the sample solution to the microorganism culture sheet 20 is completed, and the four side surfaces 32a, 32b, 32c, and 32d are raised to the vertical position with respect to the bottom surface 31 (see FIG. 4C).

  Thereafter, the microorganism culture sheet container 30 in which the microorganism culture sheet 20 is accommodated is placed in an incubator as a whole and cultured for a predetermined time, whereby the microorganism culture sheet 20 in which the colonies 21a are formed in the medium 21 is obtained.

  As described above, according to the present embodiment, the microorganism culture sheet container 30 is opened with the four side surfaces 32a, 32b, 32c, and 32d of the microorganism culture sheet container 30 down and the microorganism culture sheet container 30 is opened. The microorganism culture sheet 20 can be inserted into the sample 30 and a sample solution can be added to the microorganism culture sheet 20. For this reason, it is possible to easily perform the operation of inserting the microorganism culture sheet 20 into the microorganism culture sheet container 30 and the operation of adding the sample solution. Further, the plurality of microorganism culture sheets 20 can be reliably stacked in the microorganism culture sheet container 30 while being positioned.

  In each of the above-described embodiments, the example in which all the side surfaces 32a, 32b, 32c, 32d of the microorganism culture sheet container 30 rotate with respect to the bottom surface 31 is shown, but not limited thereto, at least one side surface, For example, only the side surface 32a may be configured to rotate with respect to the bottom surface 31.

11 rectangular waterproof sheet 13 cover sheet 14 frame 20 microorganism culture sheet 20A microorganism culture sheet main body 21 medium region 21a colony 22 non-medium region 30 microorganism culture sheet container 30A box 31 bottom surface 32a, 32b, 32c, 32d side surface 35 Joint part 36 Locking part 37 Prop

Claims (5)

A microorganism culture sheet container for stacking and storing a plurality of microorganism culture sheets having a medium configured to culture microorganisms,
Including a box having a bottom surface and a plurality of side surfaces surrounding the bottom surface;
A container for a microorganism culture sheet, wherein at least one side surface is rotated with respect to the bottom surface via a rotating portion to open the box.   The container for a microorganism culture sheet according to claim 1, wherein the rotating side surface of the box rotates within a range of 0 ° to 90 ° with respect to the bottom surface.   2. The rotating side surface of the box has one of a protruding portion and a receiving portion, and the other side of the protruding portion and the receiving portion is provided on a side surface adjacent to the rotating side surface. The container for microorganism culture sheets as described. An opening is formed in the microorganism culture sheet,
The container for a microorganism culture sheet according to any one of claims 1 to 3, wherein a column to be inserted into the opening of the microorganism culture sheet is provided on the bottom surface of the box. A microorganism culture sheet container for storing a plurality of microorganism culture sheets having a medium configured to culture microorganisms, the container including a box having a bottom surface and a plurality of side surfaces surrounding the bottom surface, and at least one In the method for using the container for a microorganism culture sheet in which the side surface is rotated with respect to the bottom surface via the rotation unit to open the box,
Rotating the rotatable side surface with respect to the bottom surface to open the box;
A method for using a container for a microorganism culture sheet, comprising the step of inoculating a medium of the microorganism culture sheet in the opened box with a sample solution.

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