JP2005143474A - Simple small-sized culturing machine - Google Patents

Simple small-sized culturing machine Download PDF

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JP2005143474A
JP2005143474A JP2003417286A JP2003417286A JP2005143474A JP 2005143474 A JP2005143474 A JP 2005143474A JP 2003417286 A JP2003417286 A JP 2003417286A JP 2003417286 A JP2003417286 A JP 2003417286A JP 2005143474 A JP2005143474 A JP 2005143474A
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Masashi Takahashi
正士 高橋
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/16—Vibrating; Shaking; Tilting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/54—Constructional details, e.g. recesses, hinges hand portable

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Abstract

<P>PROBLEM TO BE SOLVED: To provide an easily operable, small-sized and portable simple culturing machine. <P>SOLUTION: This simple culturing machine is provided by arranging/installing a thermostat part 3 equipping with both of the functions of shaking culture and stationary culture, and a storing part 9 for storing various culturing tools and devices together. In the thermostat part 3 of the simple small-sized culturing machine, the shaking culture and stationary culture can be performed at the same time. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

この発明は、簡易小型培養機に関し、更に詳細には、振盪培養機能と静置培養機能との２つの機能を共に具備している簡易小型培養機に関する。  The present invention relates to a simple small-sized incubator, and more particularly, to a simple small-sized incubator having both a shaking culture function and a stationary culture function.

細菌の培養方法としては、液体培地と固形培地が、その目的によって使い分けて用いられている。一般に、酸素を要求する好気性細菌は、液体培地中で増殖させるが、その増殖は、酸素の供給量に依存するので、空気を効果的に培地に混入することが重要な因子となる。このため、培養液は振盪するのが便利であり、そのための各種の装置が開発されている（例えば、特許文献１、２、３、４）。
一方、寒天プレート上での細菌の固形培養は、複数枚のシャーレを用いて、いわゆる孵卵器型の培養機を用いて行われるのが一般的である。したがって、振盪機能を有する機器と、培養機能を有する培養器との２つの装置が必要である。
しかしながら、上記のような従来の培養システムでは、振盪機器と培養機器とを直接一体化しようとしても持ち運びができるような携帯式の簡易小型培養機にすることは困難である。
特開平５−１３０８５９号公報 特開平９−１５４５６５号公報 特開平９−１８７２６９号公報 特開２０００−１７５６７８号公報 As a method for culturing bacteria, a liquid medium and a solid medium are selectively used depending on the purpose. In general, aerobic bacteria that require oxygen are grown in a liquid medium. Since the growth depends on the supply amount of oxygen, it is an important factor to effectively mix air into the medium. For this reason, it is convenient to shake the culture solution, and various apparatuses for this purpose have been developed (for example, Patent Documents 1, 2, 3, and 4).
On the other hand, the solid culture of bacteria on an agar plate is generally carried out using a so-called incubator type incubator using a plurality of petri dishes. Therefore, two devices are necessary: an instrument having a shaking function and an incubator having a culturing function.
However, in the conventional culture system as described above, it is difficult to obtain a portable simple small culture machine that can be carried even if the shaking device and the culture device are directly integrated.
Japanese Patent Laid-Open No. 5-130859 JP 9-154565 A JP-A-9-187269 JP 2000-175678 A

本発明者は、携帯可能な簡易小型培養機を提供するために種々検討した結果、携帯可能な小型の匡体中に振盪培養機能と静置培養機能を具備することに成功して、この発明を完成するに至った。
したがって、この発明は、振盪培養機能と静置培養機能をーとを併せ持った持ち運び可能な携帯式の簡易小型培養機を提供することを目的とする。As a result of various investigations to provide a portable simple small-sized incubator, the present inventor succeeded in providing a shaking culture function and a stationary culture function in a portable small-sized casing. It came to complete.
Accordingly, an object of the present invention is to provide a portable and simple portable incubator having both a shaking culture function and a stationary culture function.

上記目的を提供するために、この発明は、振盪培養機能と静置培養機能とを同一匡体中に具備する携帯可能な小型の簡易培養機を提供する。
また、この発明は、その別の態様として、匡体中に恒温部を設けて、同恒温部内に振盪培養機能と静置培養機能とを併せ持つ携帯可能な簡易式の小型培養機を提供する。In order to provide the above object, the present invention provides a portable and simple portable culturing machine having a shaking culture function and a stationary culture function in the same body.
As another aspect of the present invention, there is provided a portable small-sized incubator having a constant temperature part in a housing and having both a shaking culture function and a stationary culture function in the constant temperature part.

更に、この発明は、その好ましい態様として、振盪培養機能を有する振盪器に加温装置を併設することによって、所定の温度で加温しながら培養をすることができるように配設している携帯可能な簡易式の小型培養機を提供する。  Furthermore, as a preferred embodiment of the present invention, a portable device is provided so that culture can be performed while heating at a predetermined temperature by adding a heating device to a shaker having a shaking culture function. Provide a simple and small culture machine that can be used.

その上、この発明は、その好ましい態様として、匡体を２つに分割して、一方には静置培養機能と振盪培養機能とを具備させるとともに、他方には培養に必要な各種培養器具等を保管することができる保管機能を設けている携帯可能な簡易式の小型培養機を提供する。  In addition, as a preferred embodiment of the present invention, the rod is divided into two, one having a stationary culture function and a shaking culture function, and the other having various culture tools necessary for culture, etc. Provided is a portable and simple portable incubator provided with a storage function capable of storing

この発明に係る携帯式の小型培養機は、例えば、各種培養器具や装置をその中に収納することができるスーツケースなどのような箱型の匡体から構成されているのがよい。この匡体は、培養に適した恒温に調節ができるように設定されている。このように培養機自体を恒温に調節できるようにすることによって、同一培養機内に振盪培養機能と静置培養機能とを共に配設することができると共に、培養機そのものを小型化するとともに、携帯可能にコンパクト化することができることになる。なお、細菌などの微生物や培養対象物の培養に適した恒温とは、一般的には、３０℃〜３７℃程度であるのがよいが、当然の事ながら、培養する微生物その他の培養対象物などにより最適培養温度は異なるけれども、当該技術分野の当業者にとっては自明のこととして適宜設定することができる。  The portable small-sized incubator according to the present invention is preferably composed of, for example, a box-shaped casing such as a suitcase in which various culture instruments and devices can be accommodated. This rod is set so that it can be adjusted to a constant temperature suitable for culture. In this way, by allowing the incubator to be adjusted to a constant temperature, both the shaking culture function and the stationary culture function can be arranged in the same incubator, and the incubator itself can be miniaturized and portable. It will be possible to make it compact. The constant temperature suitable for culturing microorganisms such as bacteria and culture objects is generally about 30 ° C. to 37 ° C. Naturally, microorganisms to be cultured and other culture objects. Although the optimum culture temperature differs depending on the above, it can be appropriately set as obvious to those skilled in the art.

この発明の携帯式の小型培養機においては、その匡体の上側部に持ち運びが容易にできるように把手などを取り付けても、また、その底部にキャリアーを取り付けても便利である。  In the portable small-sized incubator of the present invention, it is convenient to attach a handle or the like to the upper part of the casing so that it can be easily carried and to attach a carrier to the bottom.

この発明の小型培養機において、振盪培養機能は、特に液体培地に空気を効果的に混入させて、培養を効率よく行なうためのものであり、一般的には、振盪器によって付与することができる。かかる振盪器の例としては、シェーカーなどが挙げられるが、その形状や大きさは小型培養機の匡体内に配置することができかつこの発明の目的に適うものであれば特に限定されるものではない。また、この振盪器は、それに配置するプレートなど培養器具の培地内に空気を効果的に混入できるように振盪するために所定の往復運動ができる小型のものであるのがよい。なお、かかる振盪器は、回転運動ができるものでも使用可能であるが、小型の匡体内に配置するという制約があるので、所定の往復運動ができる小型のものが好ましい。更に、かかる振盪器を所定の方向に運動操作するには、通常のモーターを匡体に配設すればよい。  In the small incubator of the present invention, the shaking culture function is for effectively mixing air, particularly in a liquid medium, and culturing efficiently, and can be generally given by a shaker. . Examples of such a shaker include a shaker and the like, but the shape and size thereof are not particularly limited as long as they can be arranged in the body of a small incubator and meet the purpose of the present invention. Absent. Moreover, this shaker should be a small one that can perform a predetermined reciprocating motion in order to shake air so that air can be effectively mixed into the culture medium of a culture device such as a plate placed thereon. Such a shaker can be used even if it can rotate, but since there is a restriction that it is placed in a small casing, a small one that can perform a predetermined reciprocating motion is preferable. Furthermore, in order to move the shaker in a predetermined direction, a normal motor may be provided in the housing.

このように配置した振盪器には、加温装置を配置するのがよい。上述したように、振盪器は所定の温度に設定された匡体の恒温部に配設されているが、微生物などの培養対象物によっては、その設定恒温よりもより高温で培養することが最適なものも存在するので、かかる培養対象物の培養の場合には、加温装置を用いて所定の温度に設定することもできるようにするのがよい。かかる加温装置としてはヒーターを使用するのがよい。  It is good to arrange | position a heating apparatus to the shaker arrange | positioned in this way. As described above, the shaker is disposed in the constant temperature part of the enclosure set to a predetermined temperature, but depending on the culture object such as microorganisms, it is optimal to culture at a higher temperature than the set constant temperature. Therefore, in the case of culturing such a culture object, it is preferable that a predetermined temperature can be set using a heating device. As such a heating device, a heater is preferably used.

また、この発明の小型培養機の恒温部には、静置培養機能を有する装置を配置するのがよい。かかる装置としては、培養用具である培地を注入したプレートなどを収納することができるようにプレートスタンドなどを配設するのがよい。このプレートスタンドにプレートを配置して所定温度で培地を静置培養することができる。  Moreover, it is good to arrange | position the apparatus which has a stationary culture function in the constant temperature part of the small culture machine of this invention. As such an apparatus, it is preferable to arrange a plate stand or the like so as to accommodate a plate or the like into which a culture medium as a culture tool has been injected. A plate can be placed on the plate stand and the medium can be statically cultured at a predetermined temperature.

この発明において、上述したように、小型培養機の恒温部に、振盪培養機能を有する振盪器と、静置培養機能を有するプレートスタンドを配設することにより、この発明の小型培養機は、固形培養と液体培養とを恒温で同時に行うことができる。  In the present invention, as described above, by arranging the shaker having a shaking culture function and the plate stand having a stationary culture function in the constant temperature part of the small culture machine, Culture and liquid culture can be performed simultaneously at a constant temperature.

また、この発明の小型培養機には、その恒温部に隣接して、例えば、プレート、培地入りチューブ、ファージ液などの各種培養用具や機器をストック用として保管することができる保管部が設けられるのがよい。この保管部は、複数段に分割して、各段にプレート、培地入りチューブおよびファージ液をそれぞれ収納できるようにするのがよい。  In addition, the small-sized culture machine of the present invention is provided with a storage unit that can store various culture tools and equipment such as plates, medium-containing tubes, phage solutions, etc., as stock, adjacent to the constant temperature unit. It is good. This storage section is preferably divided into a plurality of stages so that each stage can accommodate a plate, a medium-containing tube and a phage solution, respectively.

上記のような構成によって、この発明に係る小型培養機は、持ち運ぶことができることから、例えば、食中毒が発生した現場などに運んでいって検体を、必要に応じて、静置培養または振盪培養にて培養することにより食中毒原因菌を迅速に検出することができるという大きな利点がある。  With the configuration as described above, the small incubator according to the present invention can be carried.For example, the sample is carried to the site where food poisoning has occurred, and the specimen is subjected to stationary culture or shaking culture as necessary. This is a great advantage that food poisoning-causing bacteria can be quickly detected.

以下、この発明に係る携帯式の小型培養機の実施例について図面を参照しながら詳細に説明する。なお、下記実施例は単にこの発明を例示するためだけに記載するものであって、この発明は下記実施例に一切限定されるものではない。  Hereinafter, embodiments of a portable small-sized incubator according to the present invention will be described in detail with reference to the drawings. In addition, the following Example is described only in order to illustrate this invention, This invention is not limited to the following Example at all.

図示するように、この発明の携帯式小型培養機は、各種培養器具や装置を収容し配置することができる容器から構成されていて、その小型培養機は、例えば、手提げ型、スーツケース型の匡体１からなっているのがよい。なお、その匡体１の形状は、特に限定されてなく、この発明の目的にかなうものであればいずれの形状であってもよい。この匡体１の前側面１ａは開放自在になっていて、その前側面１ａを開けることにより、各種培養器具や装置などの出し入れや、装置の操作ができるようになっている。また、その側面１ａを閉めると、匡体１内の恒温部３を恒温にすることができ、恒温での培養を行なうことができる。
更に、この匡体１には、容易に携帯することができるように、その上面に取手２を設けても、または、その底面にキャリアー（図示せず）を設けてもよい。As shown in the figure, the portable small-sized incubator according to the present invention is composed of a container that can accommodate and arrange various culture instruments and devices. It is good to consist of the housing 1. The shape of the casing 1 is not particularly limited and may be any shape as long as it meets the object of the present invention. The front side surface 1a of the housing 1 is freely openable, and by opening the front side surface 1a, various culture instruments and devices can be taken in and out and the device can be operated. Moreover, when the side surface 1a is closed, the constant temperature part 3 in the housing 1 can be made constant temperature, and culture | cultivation at a constant temperature can be performed.
Further, the housing 1 may be provided with a handle 2 on its upper surface or a carrier (not shown) on its lower surface so that it can be easily carried.

図１に示すように、匡体１にはその内部に恒温部３が設けられていて、恒温部３には、振盪培養機能を有する培養器としてのシェーカー４が設けられている。シェーカー４は、モーター（図示せず）に電気的に結合されていて、所定の運動操作を行うことにより、そのシェーカー４に配置された液体培地入りチューブ５を所定の振盪培養を行うことができる。なお、シェーカー４の運転操作開始／終了は電源スイッチ６によって行うことができる。  As shown in FIG. 1, a thermostat 3 is provided in the housing 1, and the thermostat 3 is provided with a shaker 4 as an incubator having a shaking culture function. The shaker 4 is electrically coupled to a motor (not shown), and by performing a predetermined motion operation, the liquid medium-containing tube 5 disposed in the shaker 4 can be subjected to a predetermined shaking culture. . The start / end of the driving operation of the shaker 4 can be performed by the power switch 6.

シェーカー４の下部には、加温装置としてのヒーター７が配置されていて、必要に応じて、培地入りチューブ５を配置したシェーカー４を所定の温度に加温することができる。  A heater 7 as a heating device is disposed below the shaker 4, and the shaker 4 in which the medium-containing tube 5 is disposed can be heated to a predetermined temperature as necessary.

また、匡体１の恒温部３には、複数段を有するプレートスタンド８が更に配設されていて、各段にプレートまたは培地入りチューブを収容して静置培養することができるようになっている。  The thermostat 3 of the housing 1 is further provided with a plate stand 8 having a plurality of stages, and a plate or a medium-containing tube can be accommodated in each stage for stationary culture. Yes.

匡体１には、恒温部３に隣接して保管部９を設けるのがよい。この保管部９は、複数段、例えば、３段に分割して、例えば、プレート１０、培地入りチューブ１１、例えば複数種類のファージ液１２などの各種培養用具や機器をストック用として保管することができるようにするのがよい。なお、この保管部は、恒温にしたりまたは加温したりする必要はないので、温度調節機能を持たせる必要はない。    The housing 1 is preferably provided with a storage unit 9 adjacent to the constant temperature unit 3. The storage unit 9 is divided into a plurality of stages, for example, three stages, and can store various culture tools and equipment such as a plate 10 and a medium-containing tube 11 such as a plurality of types of phage solutions 12 as stock. It is good to be able to do it. In addition, since this storage part does not need to make it constant temperature or warm, it is not necessary to give a temperature control function.

図４は、この発明に係る小型培養機の別の実施例を示している。この別の実施例に係る小型培養機には、保管部９が設けられてなく、恒温部３だけが設けられている構成になっている。恒温部３には、前記実施例と同様に、匡体中に振盪培養機能と静置培養機能とが装備されている。  FIG. 4 shows another embodiment of the small incubator according to the present invention. The small-sized incubator according to another embodiment has a configuration in which the storage unit 9 is not provided and only the constant temperature unit 3 is provided. The thermostat 3 is equipped with a shaking culture function and a stationary culture function in the enclosure, as in the above embodiment.

この発明に係る小型培養機は、匡体中に振盪培養機能と静置培養機能とを共に具備するとともに、操作が簡便でかつ小型であり、携帯可能であるという大きな利点があり、検査現場で使用することができる。  The small incubator according to the present invention has both a shaking culture function and a stationary culture function in the rod body, and has a great advantage that it is easy to operate, small in size, and portable. Can be used.

この発明の小型培養機を示す斜視図The perspective view which shows the small incubator of this invention この発明の小型培養機を示す部分断面正面図Partial sectional front view showing a small incubator of the present invention この発明の小型培養機を示す部分断面側面図Partial sectional side view showing a small incubator of the present invention この発明の別の実施例に係る小型培養機を示す部分断面正面図Partial sectional front view showing a small incubator according to another embodiment of the present invention

符号の説明Explanation of symbols

１ 匡体
２ 取手
３ 恒温部
４ シェーカー
５ 培地入りチューブ
６ 電源スイッチ
７ ヒーター
８ プレートスタンド
９ 保管部
１０ プレート
１１ 培地入りチューブ
１２ ファージ液DESCRIPTION OF SYMBOLS 1 Housing 2 Handle 3 Constant temperature part 4 Shaker 5 Medium containing tube 6 Power switch 7 Heater 8 Plate stand 9 Storage part 10 Plate 11 Medium containing tube 12 Phage solution

Claims (7)

振盪培養機能と静置培養機能との２つの機能を共に具備していることを特徴とする携帯式小型培養機。  A portable small-sized incubator characterized by having both a shaking culture function and a stationary culture function. 請求項１に記載の携帯式小型培養機において、前記培養機は所定の恒温に調節できる恒温部を配置していることを特徴とする携帯式小型培養機。  The portable small-sized incubator according to claim 1, wherein the incubator is provided with a constant temperature part that can be adjusted to a predetermined constant temperature. 請求項１または２に記載の携帯式小型培養機において、前記２つの機能が該恒温部に配設されていることを特徴とする携帯式小型培養機。  The portable small-sized incubator according to claim 1 or 2, wherein the two functions are arranged in the constant temperature part. 請求項１ないし３のいずれか１項に記載の携帯式小型培養機において、前記振盪培養機能は、所定の往復運動もしくは回転運動をするように配設された振盪装置によって提供されることを特徴とする携帯式小型培養機。  The portable small-sized incubator according to any one of claims 1 to 3, wherein the shaking culture function is provided by a shaking device arranged to perform a predetermined reciprocating motion or rotating motion. A portable small culture machine. 請求項１ないし４のいずれか１項に記載の携帯式小型培養機において、前記振盪装置には加温装置が併設されていることを特徴とする携帯式小型培養機。  The portable small-sized incubator according to any one of claims 1 to 4, wherein the shaking device is provided with a heating device. 請求項１ないし３のいずれか１項に記載の携帯式小型培養機において、前記静置培養機能は、培養用具を収容する収容部によって提供されていることを特徴とする携帯式小型培養機。  The portable small-sized incubator according to any one of claims 1 to 3, wherein the stationary culture function is provided by a storage unit that stores a culture tool. 請求項１ないし６のいずれか１項に記載の簡易培養機において、前記培養機には更に培養に必要な各種器具を収容するための保管機能を具備していることを特徴とする携帯式小型培養機。  The portable small-sized incubator according to any one of claims 1 to 6, wherein the incubator further includes a storage function for storing various instruments necessary for culture. Incubator.
JP2003417286A 2003-11-11 2003-11-11 Simple small-sized culturing machine Pending JP2005143474A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102006004157A1 (en) * 2006-01-30 2007-08-02 Eppendorf Ag Device for incubating cells, comprises a sterile or sterilizable portable container, which encloses an integrated- and/or culture container for the reception of the cells, and an arrangement having a sterile filter and incubator
JP2009125062A (en) * 2007-11-28 2009-06-11 Hiyoshi:Kk Simple portable incubator
JP2015089355A (en) * 2013-11-06 2015-05-11 大日本印刷株式会社 Container for microorganism culture sheet, and method of using the same

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102006004157A1 (en) * 2006-01-30 2007-08-02 Eppendorf Ag Device for incubating cells, comprises a sterile or sterilizable portable container, which encloses an integrated- and/or culture container for the reception of the cells, and an arrangement having a sterile filter and incubator
US8323957B2 (en) 2006-01-30 2012-12-04 Eppendorf Ag Device and method for the incubation of cells
JP2009125062A (en) * 2007-11-28 2009-06-11 Hiyoshi:Kk Simple portable incubator
JP2015089355A (en) * 2013-11-06 2015-05-11 大日本印刷株式会社 Container for microorganism culture sheet, and method of using the same

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