JP2015064292A - Analyte analyzing device, analyte analyzing system, and monitoring method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an analyte analyzing device, analyte analyzing system, and monitoring method capable of analyzing and monitoring changes in analyte components over time, based on daily movements of a test animal.SOLUTION: An analyte analyzing device 20 includes: an administration line 30 that is attached to a test animal A and in which an administration object to be administered to the test animal is movable; a sampling line 40 that is attached to the test animal and in which a test substance sampled from the test animal is movable; an administration object feeding section 50 that feeds the administration object via the administration line; an analyte analyzing section 60 that analyzes the analyte of the test substance sent via the sampling line; and a line holding section 70 that holds the administration line and the sampling line at a position separated from the test animal and moves the administration line and the sampling line following the movement of the test animal.

Description

  The present invention relates to an analyte analyzer, an analyte analysis system, and a monitoring method.

  In the field of drug and food development, before using the developed drug or food to humans, administer it to a test animal such as a mouse to verify the effects of certain items (blood components, blood pressure, body temperature, etc.) Yes. In the test for verification, for example, the test animal is stored in a cage as described in Patent Document 1, the administration is administered to the test animal over a predetermined period, and the administration is administered to the test animal. Investigate the effects. In such a test, when administering a dose to a test animal or collecting blood as a sample from the test animal, the test animal may be anesthetized to facilitate handling of the test animal. In addition, the test animal may be killed, and blood may be collected immediately after the sacrifice.

JP 2012-29619 A

  The results of tests performed on specimens collected from anesthetized test animals and specimens collected from slaughtered test animals may be directly applicable when examining the effects on the human body. Due to the difference in influence and physiological state, a result different from the influence on the human body in a normal state may be obtained. For this reason, it is often difficult to apply the test results as they are to the human body. Moreover, about the item which changes continuously with life rhythms, such as a blood glucose level, blood pressure, and body temperature, an accurate result may not be obtained from the test animal under anesthesia and the test animal killed.

  Therefore, an object of the present invention is to provide an analyte analyzer, an analyte analysis system, and a monitoring method that enable analysis and monitoring of changes in analyte components over time in accordance with the daily operation of a test animal. This is the issue.

  The present invention is achieved by any of the following means (1) to (7).

  (1) An administration line attached to a test animal and capable of moving a dose administered to the test animal, a collection line attached to the test animal and capable of moving a sample collected from the test animal, and the administration line A dose supply unit for supplying the dose via the collection line, an analyte analysis unit for analyzing the analyte of the specimen sent via the collection line, the administration line at a position separated from the test animal, and the An analyte analyzer comprising: a line holding unit that holds a collection line and moves the administration line and the collection line to follow the movement of the test animal.

  (2) The administration line is extended in a direction away from the subject animal from the first administration line portion extended between the connection portion attached to the subject animal and the line holding portion, and the line holding portion. A second administration line section, and the sampling line extends between a connection section attached to the test animal and the line holding section, and the line holding section A second sampling line portion extending in a direction away from the subject animal, and the line holding portion relatively rotates the first administration line portion and the second administration line portion. And (1) having a connection mechanism that connects the first line sampling unit and the second sampling line unit so that they can rotate relatively and communicate with each other. Analytical analyzer described in

  (3) The above (1) or (2) including a support member that supports the line holding unit at a position separated from the test animal while applying a force in a direction away from the test animal to the line holding unit. Analytical analyzer described in 1.

  (4) The support member according to (3), wherein the support member is supported on the upper side of the test animal to suspend the administration line and the collection line on the upper side of the test animal. Analytical analyzer.

  (5) A blood glucose measurement sensor attached to the subject animal for measuring the blood glucose level of the subject animal over time, and / or attached to the subject animal for measuring the body temperature and blood pressure of the subject animal over time. The analyte analyzer according to any one of (1) to (4), further including a vital measurement sensor.

  (6) The analyte analyzer according to any one of (1) to (5) above, and a control unit that controls the operation of each unit of the analyte analyzer, An analyte analysis system that operates the analyte analyzer so that supply of the administration, collection of the sample, and analysis of the analyte of the sample are performed over time.

  (7) A monitoring method for monitoring a change in analyte of a sample collected from a test animal to which a predetermined dose is administered over time, the administration attached to the test animal for administering the dose The administration of the administration and the collection of the specimen are performed over time in a state in which the line and the collection line attached to the subject animal to collect the specimen are held movably following the movement of the subject animal. Monitoring method, which is carried out automatically and monitors the analyte of the specimen.

  According to the invention described in (1) above, since each line attached to the test animal moves following the movement of the test animal, administration of the administration and sample while each line is attached to the test animal Can be collected. This makes it possible to analyze and monitor changes over time in the analyte component in accordance with the daily operation of the test animal.

  According to the invention described in (2) above, each line can be smoothly moved by following the movement of the test animal by the connection mechanism provided in the line holding unit.

  According to the invention described in (3) above, it is possible to suitably protect each line from the test animal by applying a force in the direction of lifting the test animal to the test animal using the support member. .

  According to the invention described in (4) above, since each line can be arranged away from the test animal using the support member, each line can be more suitably protected from the test animal. .

  According to the invention described in (5) above, it is possible to analyze the interrelationship between changes in vital signs such as blood glucose level, blood pressure, and body temperature based on daily movements of test animals and changes in analyte over time. An analyte analyzer can be provided.

  According to the invention described in (6) above, it is possible to provide an analyte analysis system capable of automatically performing administration of a dosage, sample collection, and analysis of a sample analyte over time. .

  According to the invention described in (7) above, it is possible to provide a monitoring method capable of analyzing a change with time of the analyte component in accordance with the daily operation of the test animal.

It is a figure which simplifies and shows the whole structure of the analyte analysis system which concerns on embodiment of this invention. It is a figure which expands and shows the test animal which concerns on embodiment. FIG. 3 is an enlarged view of the line holding unit according to the embodiment, in which (A) is a front view and (B) is a rear view. 4A and 4B are diagrams for explaining the connection mechanism of the line holding unit according to the embodiment, in which FIG. 4A is a cross-sectional view of the line holding unit along line 4A-4A in FIG. ) Is a cross-sectional view of the line holding portion taken along line 4B-4B in FIG. It is a figure for demonstrating the effect | action of the support member which concerns on embodiment. It is a figure which shows the result of an Example.

  Embodiments of the present invention will be described below with reference to the drawings. In addition, the dimension ratio of drawing is exaggerated on account of description, and may differ from an actual ratio.

  FIG. 1 is a diagram showing an overall configuration of an analyte analysis system according to an embodiment, and FIGS. 2 to 6 are diagrams for explaining the configuration of each part of the analyte analyzer.

  As shown in FIG. 1 and FIG. 2, the analyte analyzer 20 according to the present embodiment can be summarized as an administration line 30 attached to the test animal A and capable of moving the administration administered to the test animal A, A sample line attached to the test animal A and capable of moving a sample collected from the test animal A, a dose supply unit 50 for supplying a dose via the administration line 30, and a sample sent via the sample line 40 Analyte analysis unit 60 for analyzing the analyte and the administration line 30 and the collection line 40 are held at a position separated from the test animal A, and the administration line 30 and the collection line 40 are movable following the movement of the test animal A A line holding portion 70 (see FIGS. 3 and 4).

  The analyte analyzer 20 according to the present embodiment performs administration of the administration to the test animal A, collection of the specimen from the test animal A, and analysis of the analyte of the collected specimen over time (continuous). It is possible to do so.

  In the embodiment, a rat is used as the test animal A, but it is not particularly limited as long as it is an animal that can be a subject for investigating the effects of administration of the administration. For example, animals such as guinea pigs, pigs, dogs, and cats Can be used. In addition, although a liquid drug is used as an administration, the administration can be administered to the test animal A via the administration line 30 and can be a target for investigating the influence on the test animal A. If there is no particular limitation. For example, nutrients, foods and the like can be used. The form at the time of administration is not particularly limited, and may be liquid, gas (gas), solid (for example, granular), gel, or a mixture thereof. Further, blood is collected as a sample, but the sample is not particularly limited as long as it can be collected from the test animal A via the collection line 40. For example, urine, spinal fluid, biological tissue, etc. can be collected as a specimen.

  Each configuration of the analyte analysis system will be described.

  Referring to FIG. 1, the operation control of each unit of the analyte analyzer 20 is performed via the control unit 200. The control unit 200 and the analyte analyzer 20 constitute an analyte analysis system 10.

  The control unit 200 is configured by a general personal computer (PC). The control unit 200 transmits and receives electrical signals to and from each unit of the analyte analyzer 20 by a known communication method such as wireless communication or wired communication to perform a predetermined operation. In the analyte analysis system 10, each operation such as administration of a dose, collection of a sample, and analysis of the analyte of the sample is automatically performed based on a predetermined program incorporated in the PC. As will be described later, the analyte analysis system 10 is configured to automatically perform the supply of food to the test animal A.

  The analyte analyzer 60 provided in the analyte analyzer 20 is configured by a known fully automatic blood collection device. One end 46 of the collection line 40 is connected to the fully automatic blood collection apparatus. The blood sent to the fully automated blood collection device via the collection line 40 is analyzed for its analyte by the fully automated blood collection device. When the specimen is blood, examples of the analyte include glucose and lactic acid.

  The analyte analysis unit 60 incorporates a CPU for controlling the operation of the analyte analysis unit 60. In response to an operation command from the control unit 200, the CPU operates to execute sample collection and analysis of the analyte. Note that a system can be constructed without using a PC by using a CPU incorporated in the analyte analysis unit 60 as a control unit. It is also possible to use various electronic terminal devices or the like having a communication function instead of the PC to control the operation of the entire system. In FIG. 1, transmission and reception of data and control signals performed between the control unit 200 and each unit of the analyte analyzer 20 are conceptually indicated by arrows s.

  The analysis result obtained by the analyte analysis system 10 can be stored as data in a PC RAM or the like, output on a PC display, or output to various media such as paper. When monitoring an analysis result, each output data may be browsed in real time, and stored data can be browsed after analysis.

  When performing analyte monitoring, as shown in FIG. 1, a predetermined cage 300 for accommodating the test animal A can be used. As the cage 300, for example, a publicly known one provided with a transparent wall configured so that the inside can be visually recognized from the outside can be used. Note that the volume, outer shape, and material of the constituent members of the cage 300 are configured so that the test animal A can be accommodated and the daily operation (exercise, food, etc.) of the test animal A can be allowed. As long as it is, there is no particular limitation.

  On the floor of the cage 300, for example, a lattice-like net 311 can be arranged as shown. By using such a net 311, it is possible to form a storage space for storing the excrement etc. of the test animal A under the floor. In addition, a predetermined opening 313 for leading the lines 30 and 40, the lead wires 152, and the like to the outside of the cage 300 can be provided in the ceiling portion of the cage 300.

  The cage 300 can be provided with a food holding unit 320 capable of accommodating food (food or drink) to be supplied to the test animal A. The food holding unit 320 may be provided with an external door 321 that communicates with the outside of the cage 300 and a shutter door 323 that communicates with the inside of the cage 300.

  The external door 321 can be configured with a simple mechanical structure that opens and closes in a sliding manner, for example. The external door 321 is opened and closed when feeding food into the food holding unit 320. On the other hand, as the shutter door 323, for example, a shutter door that is configured to automatically open and close according to the timing of feeding the test animal A can be used. By performing opening / closing control of the shutter door 323, it is possible to provide food to the test animal A at an arbitrary timing. The opening / closing control of the shutter door 323 is performed by the control unit 200, for example.

  In the analyte analysis system 10 according to the present embodiment, the temporal change of the vital of the test animal A along with the temporal change of the analyte based on the specimen collected from the test animal A is targeted for monitoring. Therefore, as shown in FIG. 2, in addition to the administration line 30 for administering the administration and the collection line 40 for collecting the sample, the test animal A has a blood glucose measurement sensor 150 for measuring blood glucose. A vital measurement sensor 160 for measuring body temperature and blood pressure is attached.

  Although it does not specifically limit as the blood glucose measurement sensor 150, For example, according to the intensity | strength of the fluorescence which the electrode gel 151 which measures the glucose concentration in an intertissue liquid continuously, and the fluorescent gel embedded in the body of the test animal A emits Thus, a fluorescence sensor 156 for detecting the glucose concentration can be used in combination. As the fluorescent gel, for example, a fluorescent hydrogel that emits fluorescence (for example, wavelength 488 nm) with a light amount corresponding to the glucose concentration when irradiated with excitation light having a predetermined wavelength (for example, 405 nm) can be used. .

  As shown in FIGS. 1 and 3, the result measured by the electrode sensor 151 is converted into an electrical signal and transmitted to the measuring instrument main body 140 via a predetermined lead wire 152. On the other hand, the result measured by the fluorescence sensor 156 is transmitted to the measuring instrument main body 140 via the optical fiber 157. Although not shown, each of the irradiation unit that irradiates excitation light toward the fluorescent gel, the light receiving element that receives the fluorescence, a predetermined circuit element that calculates the glucose concentration based on the intensity of the fluorescence detected by the fluorescence sensor 156, etc. The component member can be installed at any position around or near the fluorescent sensor 156.

  The lead wire 152 and the optical fiber 157 can be covered with a resin tube or the like for protection. Moreover, in order to prevent the lead wire 152 and the optical fiber 157 from dropping off from the test animal A, each end connected to the test animal A may be fixed using tape fastening, adhesion, a gripping tool, or the like. Is possible.

  The position where the electrode sensor 151 and the fluorescence sensor 156 are attached to the test animal A is not particularly limited, but the electrode sensor 151 can be attached to the waist of the test animal A, for example. Moreover, the fluorescence sensor 156 can be attached to the ear of the test animal A, for example. By installing the fluorescent gel and the fluorescent sensor 156 on the ear having a relatively thin skin, the fluorescent sensor 156 can detect light emission of the fluorescent gel with high sensitivity.

  The measuring instrument main body 140 has a function of processing and displaying a blood glucose level measurement result. Various electronic circuits and boards are built in the measuring instrument main body 140. A display panel 141 for displaying measurement results and various operation switches 143 are provided on the front surface of the measuring instrument main body 140.

  On the back surface of the measuring instrument main body 140, a lead wire 152 connected to the electrode sensor 151 and a connection terminal 145 to which an optical fiber 157 connected to the fluorescence sensor 156 is connected are provided. In addition, a predetermined internal space through which the lines 30 and 40 are inserted is formed inside the measuring instrument main body 140 (see FIG. 3B).

  The measuring instrument main body 140 processes the measurement result received via the lead wire 152 and the optical fiber 157 and displays the result on the display panel 141. The measurement result received by the measuring instrument main unit 140 can be transmitted from the measuring instrument main unit 140 to the control unit 200 by a known communication method such as wireless communication or wired communication. The control unit 200 stores the received measurement result as data.

  As shown in FIG. 2, the vital measurement sensor 160 is embedded in the body of the test animal A. As this vital measurement sensor 160, for example, data such as measurement results is transmitted and received with a telemetry device (not shown) arranged under the cage 300 or around the cage 300 by a wireless communication method using infrared rays or the like. What can be configured can be used. The measurement result received by the telemetry device can be transmitted from the telemetry device to the control unit 200 by a known communication method such as wireless communication. The control unit 200 stores the received measurement result as data.

  Next, the configuration of each line will be described.

  Each of the administration line 30 and the collection line 40 can be configured by a hollow tube through which a predetermined administration product or sample can move. Further, for example, a flexible material made of a resin material such as polyvinyl chloride, polyethylene, polypropylene, cyclic polyolefin, or polystyrene can be used for each of the lines 30 and 40. The length, sectional shape, inner diameter, outer diameter, outer shape and the like of each line 30, 40 can be arbitrarily changed according to the object to be moved.

  As shown in FIGS. 2, 3, and 4, the administration line 30 includes a connection portion 35 connected to the test animal A, and a first administration line portion that extends between the connection portion 35 and the line holding portion 70. 31 and a second administration line portion 32 extending in a direction away from the subject animal A from the line holding portion 70 (upward direction in each figure, direction toward the administration product supply portion 50).

  As shown in FIG. 1, the end 36 of the second administration line section 32 of the administration line 30 is connected to the administration product supply section 50. The dose supply unit 50 includes a storage container 51 for storing a dose, a holding container 53 for temporarily holding a dose sent from the storage container 51 via a predetermined tube 52, and a pressure supply for the dose. And a pumping system (not shown).

  The end 36 of the second administration line portion 32 of the administration line 30 is always in communication with the holding container 53. The pumping system is configured such that the operation is controlled by the control unit 200. The control unit 200 controls the pumping system to pump the administration according to a preset timing and to administer the administration to the test animal A via the administration line 30. In addition, although it is possible to use what is suitable for the kind of dosage object used as a pumping target for a pumping system, for example, it is possible to use well-known things, such as a pump and a syringe pump for fluid pumping. it can. Moreover, the position where the dosage supply unit 50 is installed is not particularly limited, but can be installed on, for example, an indoor wall where monitoring is performed.

  As shown in FIGS. 2, 3, and 4, the collection line 40 includes a connection part 45 connected to the test animal A, and a first collection line part extended between the connection part 45 and the line holding part 70. 41 and a second sampling line portion 42 extending in a direction away from the test animal A from the line holding portion 70 (upward direction in each figure, direction toward the analyte analysis portion 60).

  As shown in FIG. 1, the end 46 of the second collection line section 42 of the collection line 40 is connected to the analyte analysis section 60. As described above, when the analyte analysis unit 60 performs the sampling operation at a predetermined timing, a predetermined sample is collected from the test animal A via the first collection line unit 41 and the second collection line unit 42. The In addition, when collecting blood as a specimen, or when collecting something to which blood can adhere, it is preferable to prime the collection line 40 with an anticoagulant. This is to prevent blood adhering in the collection line 40 from coagulating and causing a thrombus.

  The position where the administration line 30 and the sampling line 40 are attached to the test animal A is not particularly limited, but the administration line 30 can be attached to the back of the test animal A, for example. For example, when the specimen is blood, the collection line 40 can be attached to the jugular vein located around the neck. In order to prevent the administration line 30 and the collection line 40 from dropping off from the test animal A, the connecting portions 35 of the respective lines 30 and 40 connected to the test animal A using tape fastening, adhesion, gripping tools, etc. 45 can be fixed.

  As shown in FIG. 3 (B), the line portions 31 and 32 of the administration line 30 and the line portions 41 and 42 of the sampling line 40 are arranged inside the measuring instrument main body 140 and inside the second connector portion 139 described later. , And extends in a direction away from the test animal A via the inside of the predetermined fastener 141. In the present embodiment, the line holding unit 70 is installed inside the second connector unit 139.

  Each line 30, 40, the lead wire 152, and the optical fiber 157 can be bundled at one place on the upper side of the test animal A as shown in FIG. By bundling, it is possible to prevent the movement of the subject animal A due to variations in the lines 30 and 40, the lead wire 152, and the optical fiber 157. When bundling, a simple bundling member 350 as shown in the figure can be used. However, when such a bundling member 350 is used, the movement of the lines 30 and 40 is restricted by the bundling member 350. The work of bundling is performed with care taken care of.

  Next, the configuration of the line holding unit will be described.

  Referring to FIG. 1, the line holding unit 70 is a test animal when the test animal A moves (including walking, running, exercise, etc.) with the lines 30 and 40 attached to the test animal A. The lines 30 and 40 are configured to move in accordance with the operation of A. By providing the line holding unit 70 in the analyte analyzer 20, when the test animal A operates, the lines 30 and 40 are tangled or kinked, and the range in which the test animal A can act is narrowed. Is preventing.

  As shown in FIGS. 4A and 4B, the line holding unit 70 includes an upper holding member 110 and a lower holding member 120 that is rotatably engaged with the upper holding member 110. . The line holding unit 70 is provided with a connection mechanism 127 including the holding members 110 and 120. The connection mechanism 127 connects the first administration line portion 31 and the second administration line portion 32 of the administration line 30 so as to be relatively rotatable and communicated with each other. The part 41 and the second sampling line part 42 are configured to be connected so as to be relatively rotatable and to be able to communicate with each other.

  The upper holding member 110 has an upper through hole 111 passing through a substantially center position in the longitudinal direction (vertical direction in FIG. 4A), and the second administration line portion 32 of the administration line 30 extending in the longitudinal direction. Are inserted therethrough, and an engagement groove 113 in which an engagement portion 123 formed in the lower holding member 120 is rotatably engaged is formed.

  The lower holding member 120 includes a lower through-hole 121 that penetrates a substantially central position in the longitudinal direction, a lower insertion hole 122 through which the first administration line portion 31 of the administration line 30 can be inserted, and an upper holding member 110. An engaging portion 123 that is engaged with the mating groove 113 and a holding groove that faces the lower end of the upper insertion hole 112 formed in the upper holding member 110 and communicates with the lower insertion hole 122 on the lower side. 124 is formed.

  The first sampling line portion 41 and the second sampling line portion 42 of the sampling line 40 are connected to each other in the through holes 111 and 121. As a connection method, for example, a method of mechanically connecting so that the second sampling line portion 42 can rotate independently with respect to the first sampling line portion 41 can be employed. In the example of illustration, it connects by the mechanical connection form which fits both the line parts 41 and 42 with respect to the predetermined | prescribed sealing member 129. FIG. By using the seal member 129 at the connection portion, the first sampling line portion 41 and the second sampling line portion 42 are connected in a liquid-tight and air-tight manner. As the seal member 129, for example, a known member made of a resin material or the like can be used.

  The second administration line portion 32 of the administration line 30 is connected to the upper holding member 110 while being inserted into the upper insertion hole 112 of the upper holding member 110. On the other hand, the first administration line portion 31 of the administration line 30 is connected to the lower holding member 120 while being inserted into the lower insertion hole 122 of the lower holding member 120. The lower holding member 120 is rotatably connected to the upper holding member 110 through a connection mechanism 127 configured by the engaging portion 123 and the engaging groove 113. For this reason, the first administration line portion 31 connected to the lower holding member 120 rotates in conjunction with the rotation of the lower holding member 120, and the second administration line portion 32 connected to the upper holding member 110 is rotated. Rotate independently.

  As shown in FIG. 4B, the holding groove 124 is formed along the circumferential direction inside the lower holding member 120. In addition, in the figure, the position of the 1st administration line part 31 is virtually shown with a broken line.

  Referring to FIG. 4, when administering the administration to test animal A via administration line 30, administration m is introduced into holding groove 124 through second administration line portion 32. In addition, the administration m is sent to the test animal A through the first administration line unit 31 communicated with the lower side of the holding groove 124. Accordingly, as shown in the drawing, each line portion is also being operated during the operation of rotating the first administration line portion 31 relative to the second administration line portion 32 (indicated by arrows r and r ′ in the figure). The state where 31 and 32 were connected can be maintained. And since the side (the 1st administration line part 31 and the 1st collection line part 41) connected to the test animal A in each line 30 and 40 rotates independently following the operation of the test animal A, The rotation is prevented from being transmitted to the side (second administration line unit 32, second collection line unit 42) connected to the administration product supply unit 50, the analyte analysis unit 60, or the like. For this reason, the range in which the test animal A can operate can be kept wide. Therefore, it is not necessary to anesthetize the test animal A when administering the administration m and collecting the specimen, and the measurement is performed over time with the administration line 30 and the collection line 40 attached to the test animal A (continuous). Can be implemented.

  As a material constituting the line holding unit 70, for example, a hard plastic or a metal material can be used. In addition, the configuration of each part of the line holding unit 70 is not limited to the illustrated form, and as long as each line unit 31, 32, 41, 42 can be movably connected to each other, the outer shape and dimensions, the engaging unit 123. In addition, the configuration of the engagement groove 113, the shape of the holding groove 124, and the like can be changed as appropriate.

  Next, the support member will be described.

  As shown in FIG. 5, the analyte analyzer 20 applies the force in the direction away from the test animal A (separation force, arrow f <b> 1 in FIG. 5) to the line holding unit 70, while the line holding unit 70 is used as the test animal. The support member 130 is supported at a position separated from A. The support member 130 includes, for example, a support 131, an arm part 133 that extends from the support 131 with a predetermined length, a first connector part 135 that connects the support 131 and the arm part 133, and the line holding part 70. An urging portion 137 for urging the separation force and a second connector portion 139 to which the line holding portion 70 is attached and detached can be configured.

  The support 131 can be attached to the outer wall of the cage 300, for example. Further, the arm part 133 extended from the column 131 can be arranged at a position where the measuring machine main body part 140 is lifted from an obliquely upper side of the line holding part 70.

  The biasing portion 137 is configured to bias a force in a direction in which the arm portion 133 is lifted upward by, for example, an elastic force such as a spring. For this reason, the line holding unit 70 is given a force to be lifted upward via the arm unit 133. The lines 30 and 40 connected to the line holding unit 70 are lifted upward together with the line holding unit 70. As a result, a force in the direction of lifting the subject animal A is applied to the subject animal A to which the lines 30 and 40 are attached. The method of applying the lifting force to the test animal A is not limited to a form using an elastic force such as a spring. For example, a load is applied to the first connector unit 135 and the arm unit 133 and the line holding unit 70. It is also possible to adopt a method of lifting the lever by the lever principle.

  When performing various tests using the test animal A, if the test animal A is given a force for pressing the test animal A, the test animal A crawls, lies down, walks, runs, etc. May act to refrain from movement. In addition, if a small animal such as a rat is used as the test animal A, when the rat crouches, the lines 30, 40, the lead wire 152, the optical fiber 157, etc. may be bitten, scratched, or damaged. . On the other hand, when a force in the lifting direction is applied to the test animal A via the line holding unit 70, the test animal A struggles toward the floor (arrow f2 in FIG. 5) against the lifted force. I will try. Therefore, by applying a force in the lifting direction to the test animal A, it is possible to prevent the test animal A from taking a jerky action and to suitably prevent the apparatus from being damaged by the test animal A. It becomes possible.

  As shown in FIG. 5, the administration line 30 and the collection line 40 are suspended above the test animal A by supporting the line holding unit 70 above the test animal A using the support member 130. be able to. By extending the respective lines 30 and 40 to the upper side of the test animal A, the respective lines 30 and 40 can be disposed away from the test animal A, so that the respective lines 30 and 40 are more reliably protected. be able to. Moreover, it is possible to prevent the lead wire 152 and the optical fiber 157 from being damaged by suspending the lead wire 152 and the optical fiber 157 together with the lines 30 and 40 above the test animal A.

  As mentioned above, according to this embodiment, since each line 30 and 40 attached to the test animal A follows the operation | movement of the test animal A, respectively, in the state which attached each line 30 and 40 to the test animal A, It is possible to administer the administration and collect the specimen. As a result, it is possible to analyze and monitor the temporal change of the analyte component in accordance with the daily operation of the test animal A.

  Further, the connection mechanism 127 provided in the line holding unit 70 allows the lines 30 and 40 to be smoothly moved while following the operation of the test animal A.

  Moreover, each line 30 and 40 can be suitably protected from the test animal A by applying a force in the direction of lifting the test animal A to the test animal A using the support member 130.

  Moreover, since each line 30 and 40 can be arrange | positioned away from the test animal A by suspending each line 30 and 40 above the test animal A using the support member 130, each line 30 , 40 can be suitably protected from test animal A.

  In addition, by using the blood glucose measurement sensor 150 that measures the blood glucose level of the test animal A over time and the vital measurement sensor 160 that measures the body temperature and blood pressure of the test animal A over time, the test animal A can perform daily operations. It is possible to provide an analyte analyzer 20 that can analyze the correlation between changes in vital signs such as blood glucose level, blood pressure, and body temperature and changes in analyte over time.

  In addition, it is possible to provide the analyte analysis system 10 that can automatically perform administration of a dosage, sample collection, and analysis of a sample analyte over time.

  In addition, it is possible to provide a monitoring method capable of analyzing a change with time of an analyte component in accordance with the daily operation of the test animal A.

<Example>
In FIG. 6, the measurement result which measured the blood glucose level using the electrode sensor is shown. In addition, the apparatus, system, monitoring method, etc. which concern on this invention are not limited to the content as described in an Example.

  The vertical axis in the graph indicates the glucose concentration in the interstitial fluid detected by the electrode sensor, the blood glucose level (mmg / dl) measured based on the glucose concentration, and the amount of food supplied to the test animal A (g). Is shown. The horizontal axis in the graph indicates the period (time) during which the measurement was performed.

  Rat A was used as test animal A. Further, the analyte analysis system 10 described in the above-described embodiment was used, and monitoring was continuously performed for 72 hours without using anesthesia during measurement.

  Referring to the measurement results, it can be confirmed that after the rat ingests the food, the blood glucose level of the rat increases according to the intake of the food. By using the monitoring system according to the above-described embodiment, it was possible to continuously analyze and monitor the correlation between the intake of food and the change in blood glucose level.

  As described above, the present invention has been described through the embodiment. However, the present invention is not limited to the configuration described in the embodiment, and can be modified based on the description of the scope of claims.

  For example, although the embodiments using the blood glucose measurement sensor 150 and the vital measurement sensor 160 have been described, the use of these sensors can be omitted, and the configuration using either one of the sensors can be adopted. Is possible. Moreover, although the example which uses two types of sensors, the electrode sensor 151 and the fluorescence sensor 156, was demonstrated as the blood glucose measurement sensor 150, it is also possible to measure a blood glucose level using one sensor. Further, for example, the use of the support member 130 can be omitted.

10 Analytical analysis system,
20 Analytical analyzer,
30 administration line,
35 connections,
31 First administration line section,
32 Second administration line section,
40 sampling line,
45 connections,
41 First sampling line section,
42 Second sampling line section,
50 dose supply unit,
60 Analytical Analysis Department,
70 line holder,
127 connection mechanism,
130 support members,
140 Measuring instrument body,
150 blood glucose sensor,
151 electrode sensor,
160 vital measurement sensor,
200 control unit,
300 cages,
320 food holding unit,
A Test animal.

Claims (7)

An administration line attached to the test animal, to which the administration to be administered to the test animal is movable;
A sampling line attached to the test animal and capable of moving a sample collected from the test animal;
A dose supply unit for supplying the dose via the administration line;
An analyte analyzer that analyzes the analyte of the specimen sent through the collection line;
An analyte analyzer comprising: a line holding unit that holds the administration line and the collection line at a position separated from the subject animal, and moves the administration line and the collection line in accordance with the movement of the subject animal. The administration line is
A first administration line portion extending between the connection portion attached to the subject animal and the line holding portion; a second administration line portion extending in a direction away from the subject animal from the line holding portion; Have
The sampling line is
A first sampling line portion extending between the connection portion attached to the test animal and the line holding portion; a second sampling line portion extending in a direction away from the test animal from the line holding portion; Have
The line holding unit connects the first administration line unit and the second administration line unit so that the first administration line unit and the second administration line unit can be relatively rotated and communicated with each other, and the first line collection unit and the second collection line unit The analyte analyzer according to claim 1, further comprising a connection mechanism for connecting the two in a rotatable manner and in a communicable manner.   The analyte according to claim 1, further comprising a support member that supports the line holding unit at a position separated from the test animal while applying a force in a direction away from the test animal to the line holding unit. Analysis equipment.   The analyte analyzer according to claim 3, wherein the support member supports the holding unit above the subject animal, thereby suspending the administration line and the collection line above the subject animal. .   A blood glucose measurement sensor attached to the subject animal for measuring the blood glucose level of the subject animal over time, and / or a vital measurement attached to the subject animal for measuring the body temperature and blood pressure of the subject animal over time. The analyte analyzer according to any one of claims 1 to 4, further comprising a sensor. The analyte analyzer according to any one of claims 1 to 5,
A control unit for controlling the operation of each unit of the analyte analyzer,
The analyte analysis system, wherein the control unit operates the analyte analyzer so that supply of the administration product, collection of the sample, and analysis of the analyte of the sample are performed over time. A monitoring method for monitoring a change in analyte in a specimen collected from a test animal to which a predetermined administration is administered over time,
The administration line attached to the test animal for administering the administration and the collection line attached to the test animal for collecting the specimen are held movably following the movement of the test animal. A monitoring method in which administration of the administration product and collection of the specimen are performed over time in the state, and the analyte of the specimen is analyzed.