JP2015059103A - Muscular glycogen accumulation promoter - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a muscular glycogen accumulation promoter which can attempt to increase the muscular glycogen accumulation amount safely and readily without exercise and injury.SOLUTION: A muscular glycogen accumulation promoter contains a processing powder of Araliaceae Panax japonicus plant. The embodiment that the processing powder of Araliaceae Panax japonicus plant is obtained by acting a strong acid aqueous solution with a concentration of 0.01-4 mol/L to the Araliaceae Panax japonicus plant and performing hydrolysis treatment under presence of lower alcohol, and the embodiment that the Araliaceae Panax japonicus plant is Panax notoginseng are preferable.

Description

  The present invention relates to a muscle glycogen accumulation promoter.

We perform physical activities such as daily life by moving muscles. However, continuation of bedridden life due to accidents or illness, aging, long-term life in a zero-gravity environment, and decreased physical activity due to other causes may lead to muscle mass loss and hinder daily life is there. Utilizing the muscle means contracting the muscle, but when the muscle contracts, first, stored glycogen in the muscle is used.
However, when the muscle mass decreases due to the above-described causes, the amount of muscle glycogen in the body decreases accordingly. Muscle glycogen is a necessary substance that serves as an energy source for muscle contraction. If the amount of glycogen accumulated in the muscle is insufficient, physical activity is impaired. Therefore, increasing the amount of glycogen accumulation in muscle leads to various benefits of physical activity such as rapid onset of movement, improvement of physical activity sustainability, recovery from fatigue and improvement of exercise ability.

Muscle glycogen is synthesized through an action mechanism such as (1) improvement of glucose uptake from blood vessels to muscle, and (2) glycogen synthesis from glucose in muscle.
However, there is an upper limit to the amount of muscle glycogen accumulated, and it is not easy to increase the amount of muscle glycogen accumulation, and intense exercise is required. Then, in order to solve the said problem, the glycogen accumulation promoter which uses a fat globule membrane | film | coat component as an active ingredient is proposed, for example (refer patent document 1). Further, a glycogen accumulation promoter containing a cyclic and / or chain polylactic acid mixture having a condensation degree of 3 to 20 has been proposed (see Patent Document 2). These proposed active ingredients have nothing to do with the Argiaceae plant.

  Therefore, a safe and uninjured muscle glycogen accumulation method has not yet been found, and muscles that can increase muscle glycogen accumulation safely and easily without being injured without applying an exercise load. It is desired to provide a glycogen accumulation promoter.

JP 2010-59155 A International Publication No. 2001/021182 Pamphlet

  An object of the present invention is to solve the above-described problems and achieve the following objects. That is, an object of the present invention is to provide a muscle glycogen accumulation promoter that can increase the amount of muscle glycogen accumulated safely and easily without injury without applying exercise load.

  The muscle glycogen accumulation promoter of the present invention as a means for solving the above-mentioned problems contains a processed powder of the genus Araceae plant.

  According to the present invention, muscle glycogen can solve the conventional problems, achieve the object, and can increase muscle glycogen accumulation safely and easily without applying an exercise load. An accumulation promoter can be provided.

FIG. 1 is a diagram collectively showing experimental methods of an initial value group, a muscle glycogen lowering group, and a muscle glycogen recovery group in Examples. FIG. 2 is a graph showing the results of measuring the amount of muscle glycogen at each timing of the initial value group, muscle glycogen reduction group, and muscle glycogen recovery group in the examples.

(Muscle glycogen accumulation promoter)
The muscle glycogen accumulation promoter of the present invention contains a processed powder of the genus Araceae, and further contains other components as necessary.

<Processed powder of urchinaceae plant genus plant (acid-treated product)>
The plant belonging to the genus Tochibanin ginseng is not particularly limited and may be appropriately selected depending on the intended purpose. For example, seven ginsengs (Denanthin ginseng, also known as: three-seven ginseng), ginseng (Ginseng ginseng) , Aka: ginseng (carrot carrot), ginseng (carrot carrot)), tochiban carrot (aka: ginseng ginseng), american carrot (aka: ginseng (carrot), ginseng)), Vietnamese carrots, Himalayan carrots, Ginseng-like carrots, Carrion ginseng, Nanaha carrots (aka Carrot, also known as: Chikukonshichi), Seiha Hanechi Ginseng (Shureikainjin, also known as: Chikutsu Sansichi), Ohba 7 (Daiyo Sanshichi), Ehime Sanchichi (Gabisanshichi), Gyosansanchichininjin (also known as: Taksanshichi), honey bean carrot, Nosanshichininjin (also known as: Kyojo Sanshichi)) Can be mentioned. Among these, sesame ginseng and ginseng are preferred, and ginseng is more preferred from the viewpoint of stable availability.

  As the plant belonging to the genus Totibandin, it may be used as it is collected from nature, but since the roots and rhizomes contain a particularly large amount of active ingredients, the roots and rhizome parts, and this were crushed It is preferable to use a powder. Among these, a powdery one is preferable from the viewpoint of efficiently performing the acid treatment described later.

The processed powder of the genus Araceae plant is more effective than the use of the Arcoceae plant.
The reason for this is that when the above-mentioned Araceae Tochibanin genus plant is acid-treated, the sugar is released from the glycoside (saponin), which is a medicinal component contained in the plant body, and panaxatriol (PT), panaxadiol (PD), proto Sapogenins (aglycone bodies) such as panaxatriol (PPT) and protopanaxadiol (PPD) are produced. These sapogenins are considered to exert the above action as active ingredients.
There is no restriction | limiting in particular as content of the said sapogenin in the said processed powder, Although it can select suitably according to the objective, 3 mass% or more is preferable, 5 mass% or more is more preferable, 10 mass% or more Is particularly preferred.
The content of the sapogenin can be measured by, for example, liquid chromatography.

  There is no restriction | limiting in particular as content of the said processed powder in the said muscle glycogen accumulation | storage promoter, According to the objective, it can select suitably. The muscle glycogen accumulation promoter may be the processed powder itself.

<Other ingredients>
There is no restriction | limiting in particular as said other component, In the range which does not impair the effect of this invention, it can select suitably according to the objective.
There is no restriction | limiting in particular also as content of the said other component in the said muscle glycogen accumulation | storage promoter, According to the objective, it can select suitably.

<Manufacturing method>
As described above, the processed powder of the genus Araceae plant is obtained by hydrolyzing the Argiaceae plant in the presence of a strong acid aqueous solution and a lower alcohol, followed by hydrofiltration.
In particular, from the viewpoint of efficiently obtaining the processed powder, it is preferable to produce the processed powder by the following production method.

That is, the processed powder is obtained by hydrolyzing the Argiaceae plant in the presence of a strong acid aqueous solution and a lower alcohol having a predetermined concentration (hydrolysis treatment step), and neutralizing the obtained hydrolyzed solution. After (neutralization step), the water is filtered (hydrofiltration step), and the filtered residue is dried (drying step).
Hereinafter, the preferable manufacturing method will be described in detail.

<< Hydrolysis treatment process >>
In the hydrolysis treatment step, the Argyceae Tochibanin ginseng plant is hydrolyzed in the presence of a strong acid aqueous solution and a lower alcohol at a predetermined concentration to produce the processed powder.

The strong acid aqueous solution is not particularly limited as long as it is an aqueous solution containing a strong acid, and can be appropriately selected according to the purpose. Among these, an aqueous solution containing an inorganic acid such as hydrochloric acid, phosphoric acid, sulfuric acid, nitric acid, etc. An aqueous solution containing hydrochloric acid is particularly preferable. The concentration of the acid in the strong acid aqueous solution is preferably 0.01 mol / L to 4 mol / L, and more preferably 0.5 mol / L to 3 mol / L.
If the acid concentration is less than 0.01 mol / L, there may be a problem that hydrolysis is insufficient and the processed powder is not efficiently produced. If the acid concentration exceeds 4 mol / L, hydrolysis proceeds too much. Or may be disadvantageous in terms of cost. On the other hand, when the acid concentration is within the preferable range, it is advantageous in that a processed powder can be efficiently obtained by sufficient hydrolysis.

It is preferable that the strong acid aqueous solution is used in a volume of 2 to 20 times the volume of the genus Tochibaninjin.
When the amount of the strong acid aqueous solution used is less than 2 times the volume of the genus Tochibanin ginseng plant, the Uchigaceae Tochibanin ginseng plant may not be sufficiently immersed and the hydrolysis treatment may be insufficient. If the capacity exceeds 20 times, it may be disadvantageous in terms of cost.

<<< Use of lower alcohol >>>
The hydrolysis treatment is performed in the presence of a lower alcohol. By using the lower alcohol, it is possible to improve the affinity between the Argiaceae plant and the strong acid aqueous solution and to promote the hydrolysis efficiently. In addition, the use of the lower alcohol is advantageous in that the taste and handleability of the obtained processed powder can be improved.
The lower alcohol is not particularly limited and may be appropriately selected depending on the intended purpose. Among these, methanol, ethanol, and propanol are preferable, and ethanol is particularly preferable from the viewpoint of safety.
In addition, in this specification, a lower alcohol shows a C1-C4 alcohol compound.

There is no restriction | limiting in particular as the usage-amount of the said lower alcohol, Although it can select suitably according to the objective, 1 volume%-80 volume% are preferable with respect to the total amount of a hydrolysis liquid, 10 volume%-50 volume % Is more preferable, and 20% by volume to 40% by volume is particularly preferable.
When the amount of the lower alcohol used is less than 1% by volume with respect to the total amount of the hydrolyzed liquid, the processed powder may not be obtained efficiently. When the amount used exceeds 80% by volume, the processed powder is efficiently obtained. There are things that cannot be obtained, and that it is disadvantageous in terms of cost. On the other hand, when the amount of the lower alcohol used is within the particularly preferable range, it is advantageous in that a processed powder can be obtained efficiently. The “total amount of hydrolyzed liquid” refers to the total amount of the reaction liquid including the strong acid aqueous solution and the lower alcohol.

The total amount of the reaction solution (total amount of hydrolysis solution) including the strong acid aqueous solution and the lower alcohol is not particularly limited and may be appropriately selected depending on the intended purpose. Thus, the capacity is preferably 2 to 20 times the capacity.
If the total amount of the reaction solution is less than 2 times the volume of the genus Tochibanin ginseng plant, it may not be sufficiently immersed and the hydrolysis treatment may be insufficient. May be disadvantageous.

There is no restriction | limiting in particular as processing temperature in the said hydrolysis process, Although it can select suitably according to the objective, 60 to 100 degreeC is preferable and 70 to 90 degreeC is more preferable.
If the treatment temperature is less than 60 ° C, hydrolysis may be insufficient and the processed powder may not be produced efficiently. If the treatment temperature exceeds 100 ° C, special production equipment is required, which is disadvantageous in cost. Sometimes. On the other hand, when the processing temperature is within the more preferable range, it is advantageous in that the processed powder can be obtained efficiently.

Moreover, there is no restriction | limiting in particular as processing time in the said hydrolysis process, Although it can select suitably according to the objective, 30 minutes-24 hours are preferable, and 2 hours-8 hours are more preferable.
If the treatment time is less than 30 minutes, hydrolysis may be insufficient and the processed powder may not be obtained efficiently. If the treatment time exceeds 24 hours, the reaction may proceed excessively or the cost may be disadvantageous. There is. On the other hand, when the processing time is within the more preferable range, it is advantageous in that the processed powder can be obtained efficiently.

<< Neutralization process >>
In the neutralization step, the obtained hydrolyzed solution is neutralized after the hydrolysis treatment.
The neutralization is not particularly limited and can be performed by a known method. For example, the neutralization is performed by appropriately adding a strong base aqueous solution such as sodium hydroxide or potassium hydroxide to the liquid after the hydrolysis treatment. Can do. In addition, it is preferable that the pH after the said neutralization shall be 5-8.

<< Hydrofiltration process >>
In the filtration step, the hydrolyzed liquid after the neutralization step is hydrofiltered and separated into a filtrate and a residue.
The hydrofiltration is not particularly limited and can be performed by a known method. After filtration, washing with water may be repeated until there is no more salt.

<<< Hydrofiltration >>>
Since the lower alcohol is used, water is added to lower the concentration of the lower alcohol in the solution after the hydrolysis treatment for the purpose of promoting the residue in the processed powder before filtration.
In this case, the lower alcohol concentration in the liquid after the hydrolysis treatment is better as it is lower. Therefore, the more water is added, the lower alcohol concentration in the liquid after the hydrolysis treatment is 50% by volume or less. It is preferable to add to 30% by volume, more preferably 30% by volume or less, and particularly preferably 10% by volume or less.
If the lower alcohol concentration in the liquid after the hydrolysis treatment is subjected to filtration while exceeding 50% by volume, it is disadvantageous in that the obtained processed powder is dissolved in the lower alcohol and discharged as a filtrate. On the other hand, it is advantageous that the processed powder can be obtained more efficiently if the lower alcohol concentration in the liquid after the hydrolysis treatment is in a particularly preferred range.

<< Drying process >>
In the drying step, the filtered residue after the hydrofiltration step is dried to obtain the purified processed powder.
There is no restriction | limiting in particular in the said drying, It can perform by a well-known method, For example, normal methods, such as freeze drying, ventilation drying, heat drying, and reduced pressure drying, can be utilized.

<Other ingredients>
The other components are not particularly limited and may be appropriately selected according to the purpose. For example, the other components may be appropriately selected from pharmacologically acceptable carriers according to the dosage form of the muscle glycogen accumulation promoter. For example, ethanol, water, starch and the like can be mentioned. Moreover, when using the said muscle glycogen accumulation | storage promoter for the food / beverage products mentioned later, as said other component, various auxiliary raw materials or additives are mentioned, for example. There is no restriction | limiting in particular also as content of the said other component, According to the objective, it can select suitably.

  The muscle glycogen accumulation improver can be used for various applications such as pharmaceuticals, quasi drugs, general foods, health foods and health drinks, health functional foods, food additives, feeds, feed additives, and the like. In this case, it can be provided as a dry powder, or it can be prepared by providing it in a liquid, tablet, powder, granule, dragee, capsule, suspension, emulsion, ampoule or any other form. You can also.

  There is no restriction | limiting in particular as a manufacturing method of the said sugar metabolism improving agent, According to dosage form etc., it can select suitably from well-known methods.

<Ingestion>
There is no restriction | limiting in particular as an ingestion method of the said muscle glycogen accumulation promoter, ingestion amount, ingestion frequency, ingestion time, and ingestion object, According to the objective, it can select suitably.
There is no restriction | limiting in particular as said ingestion method, Although it can select suitably according to the objective, The method of ingesting orally is preferable at the point which is easy to continue since it can ingest easily.
The intake amount is not particularly limited and may be appropriately selected in consideration of various factors such as the age, weight, constitution, symptom, and presence / absence of administration of a drug containing another component as an active ingredient. However, the daily intake is preferably 10 mg to 1,000 mg, more preferably 20 mg to 50 mg, and particularly preferably 30 mg to 300 mg. Within the preferable range, it is advantageous in that an excellent muscle glycogen accumulation promoting action can be exhibited.
The intake time is not particularly limited and may be appropriately selected depending on the purpose. The muscle glycogen accumulation promoter may be taken before or after exercise, or during exercise. It does not matter.
In addition, in order to reduce the annoyance related to taking for the user, the intake time should not be limited at the same time as meals or after meals, and the effect of promoting muscle glycogen accumulation can be achieved without taking it at the same time as meals. If it is a dosage form that can be ingested as a normal food without any problems, the muscle glycogen accumulation promoting effect is not different depending on the time of intake. It is not related to meals and non-simultaneous intake.
The animal species to be ingested are those that are suitably applied to humans, but as long as their effects are exerted, animals other than humans (eg, mice, rats, hamsters, birds, dogs, Cats, sheep, goats, cows, pigs, monkeys, etc.).

<Use>
The said muscle glycogen accumulation promoter may be used individually by 1 type, may use 2 or more types together, and may be used with the pharmaceutical which uses another component as an active ingredient. Moreover, the said muscle glycogen accumulation promoter may be used in the state mix | blended in the pharmaceutical which uses another component as an active ingredient.

<Application>
Since the muscle glycogen accumulation promoter of the present invention has an excellent muscle glycogen accumulation promoting action, it can also be suitably used for foods and drinks described below.
The muscle glycogen accumulation promoter can exert the effect of increasing muscle glycogen even without performing the intense exercise required to increase the amount of muscle glycogen in the past. Therefore, by administering it to administration subjects that are difficult to exercise due to exercise, such as bedridden sick people due to accidents or illness, injured or ill patients who need to rest for a certain period of time, elderly people who are difficult to exercise intensely, etc. Increase or maintenance of glycogen accumulation can be expected, and bedridden prevention and anti-aging effects can also be expected.

<Food &Drink>
The food and drink used in the present invention contains the muscle glycogen accumulation promoter, and further contains other components as necessary.
Here, the food and drink are those which are less likely to harm human health and are taken by oral or gastrointestinal administration in normal social life. It is not limited to the category of goods, etc., and means, for example, a wide range of foods that are taken orally, such as general foods, health foods, health functional foods, quasi drugs, and pharmaceuticals.

There is no restriction | limiting in particular as a compounding quantity of the said muscle glycogen accumulation | storage promoter in the said food / beverage products, It can mix | blend suitably according to the kind of food / beverage products used as long as the effect of this invention is not impaired.
The food and drink may contain only the muscle glycogen accumulation promoter, and the food and drink may be the muscle glycogen accumulation promoter itself.

-Types of food and drink-
There is no restriction | limiting in particular as said kind of food / beverage products, It can select suitably according to the objective, For example, drinks, such as a soft drink, a carbonated drink, a nutrition drink, a fruit drink, a lactic acid drink; Ice cream, ice sherbet, Frozen confectionery such as shaved ice; noodles such as buckwheat, udon, harusame, gyoza skin, sushi mai, Chinese noodles, instant noodles; rice cake, candy, gum, chocolate, tablet confectionery, snack confectionery, biscuits, jelly, jam, cream, baked Sweets such as confectionery and bread; crab, salmon, clams, tuna, sardines, shrimp, skipjack, mackerel, whale, oyster, saury, squid, red scallop, scallop, abalone, sea urchin, salmon roe, tocobushi, etc .; Processed fishery and livestock products such as sausages; Dairy products such as processed milk and fermented milk; salad oil, tempura oil, margarine, mayonnaise, shii Oil and fat processed foods such as toning, whipped cream, dressing; seasonings such as sauces, sauce; curry, stew, parent and child rice bowl, rice bowl, miscellaneous cooking, Chinese rice bowl, bowl, tempura, eel rice cake, hayashi rice, oden, marbodorf, Retort pouch foods such as beef bowl, meat sauce, egg soup, omelet rice, dumplings, shumai, hamburg, meatballs; various forms of health foods, nutritional supplements, pharmaceuticals, quasi drugs and the like.

<Other ingredients>
There is no restriction | limiting in particular as said other component, According to the objective, it can select suitably, For example, the auxiliary | assistant raw material or additive etc. which are normally used in manufacturing food-drinks are mentioned.
The auxiliary raw material or additive is not particularly limited and may be appropriately selected depending on the intended purpose. For example, glucose, fructose, sucrose, maltose, sorbitol, stevioside, rubusoside, corn syrup, lactose, citric acid , Tartaric acid, malic acid, succinic acid, lactic acid, L-ascorbic acid, dl-α-tocopherol, sodium erythorbate, glycerin, propylene glycol, glycerin fatty acid ester, polyglycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, Arabia Examples include gum, carrageenan, casein, gelatin, pectin, agar, vitamin Bs, nicotinic acid amide, calcium pantothenate, amino acids, calcium salts, pigments, fragrances, preservatives and the like.
There is no restriction | limiting in particular as content of the said other component, According to the objective, it can select suitably.

  EXAMPLES Hereinafter, although an Example is given and this invention is demonstrated in detail, this invention is not limited to these Examples at all.

(Production Example 1)
<Nanata Participant Engineering Powder (Acid Treatment)>
1 kg of ginseng powder (manufactured by Matsuura Pharmaceutical Co., Ltd.) is suspended in 10 L of a 25 wt% aqueous ethanol solution containing 5.9 wt% hydrochloric acid (2 mol / L hydrochloric acid) and reacted at 70 ° C. for 6 hours with slow stirring. I let you. Subsequently, after cooling this reaction liquid on ice, 5 mol / L sodium hydroxide aqueous solution was added and it adjusted to pH 7.0. Next, the pH-adjusted solution was diluted 10-fold with distilled water, suction filtered, and separated into a filtrate and a residue. The obtained residue was freeze-dried to obtain 180 g of Tanashi Ninjin Participant Powder.

(Example 1: Muscle glycogen accumulation promoting effect)
〔Method〕
In order to confirm the effect of promoting muscle glycogen accumulation, the amount of muscle glycogen was confirmed.
Since it is necessary to reduce the amount of muscle glycogen once to confirm the amount of muscle glycogen synthesis, it was decided to evaluate the amount of muscle glycogen synthesis by reducing the amount of muscle glycogen by applying a swimming exercise after this breeding . The amount of glycogen synthesis increased in the group that received the Tanachi Participant's powder compared to the amount of muscle glycogen synthesis in the subject group that did not receive the Tanachi Participant's powder, which was used as the muscle glycogen accumulation promoting effect.

〔animal〕
5-week-old Balb / c male mice (Nippon Charles River Co., Ltd.) were used for the experiment. Mice were raised during the test period under a 12 hour light / dark cycle, a temperature of 22 ± 2 ° C., and a relative humidity of 50 ± 5%. After carrying in, one week was set as a pre-breeding period, and during the pre-breeding period, the following intensity swimming practice was carried out once to learn the swimming method. Thereafter, for the purpose of grouping, the grouping swimming test with the following strength was applied three times over the course of one week.
-Swimming practice intensity: Swim for 10 minutes under running water of 10 L / min.
-Grouped swimming strength: Swim for 10 minutes under running water of 10 L / min, then increase the water flow rate by 1 L / min every 5 minutes, and finish swimming when staying in water for 7 seconds.
Mice satisfying both of the following conditions were selected as exercise groups.
(1) Small variation in swimming time of grouped swimming performed 3 times (2) Swimming time is close to the group average

<Initial value group>
Mice that were not selected were used as the initial value group. The initial value group was divided into two groups (a control group and a seven-participant powder group) so that the body weight and swimming time values would be averaged, and only kept in the cage during this breeding period.
Control group: n = 6 (CE-2 solid food free feeding)
・ Nanata Participant Engineering Powder Group: n = 6 (1.25% by weight Tanachi Participant Engineering Powder Mixing CE-2 Solid Feed Free Feeding: Approximately 40 mg Rice Field 7 Participation Participant Intake)

<Muscle glycogen lowering group, muscle glycogen recovery group>
The decrease and recovery group of muscle glycogen was divided into two groups (control group and Nanata Participant Powder Group) so that the body weight / swimming time values were averaged, and were reared for 4 weeks as the main breeding. During this period, in order not to forget the swimming method once a week, swimming practice was imposed and food was freely fed.
Control group: n = 6 (CE-2 solid food free feeding)
・ Nanata Participant Engineering Powder Group: n = 6 (1.25% by weight Tanachi Participant Engineering Powder Mixing CE-2 Solid Feed Free Feeding: Approximately 40 mg Rice Field 7 Participation Participant Intake)
The number of mice in each group is shown in Table 1 below.

４週間の本飼育後、初期値群である初期値−コントロール群（ｎ＝６）と、初期値−田七人参加工粉末群（ｎ＝６）は、明期に筋肉を摘出して筋肉グリコーゲン量を測定した。
筋肉グリコーゲン低下群である筋肉グリコーゲン低下−コントロール群（ｎ＝６）と筋肉グリコーゲン低下−田七人参加工粉末群（ｎ＝６）は、筋肉グリコーゲン量を低下させるため遊泳運動を処置し、遊泳運動直後に筋肉グリコーゲン量を測定することで、筋肉グリコーゲン量の低下を確認した。
筋肉グリコーゲン回復群である筋肉グリコーゲン回復−コントロール群（ｎ＝６）と筋肉グリコーゲン回復−田七人参加工粉末群（ｎ＝６）は、筋肉グリコーゲン低下処置後にグルコースを投与し、９０分間後に筋肉グリコーゲン量を測定し、筋肉グリコーゲン回復−コントロール群と筋肉グリコーゲン回復−田七人参加工粉末群の筋肉グリコーゲン量を比較することで、筋肉グリコーゲン蓄積促進効果を確認した。
図１に、初期値群、筋肉グリコーゲン低下群、及び筋肉グリコーゲン回復群の実験方法についてまとめて示した。

After the main breeding for 4 weeks, the initial value-control group (n = 6), which is the initial value group, and the initial value-Nanata Participant's powder group (n = 6), muscles were extracted in the light period by muscle glycogen. The amount was measured.
Muscle glycogen reduction group, muscle glycogen reduction group-control group (n = 6) and muscle glycogen reduction group-Nana Tanajin Participant Powder Group (n = 6) treated swimming exercise to reduce muscle glycogen content, swimming exercise Immediately after that, the amount of muscle glycogen was confirmed by measuring the amount of muscle glycogen.
Muscle glycogen recovery group, muscle glycogen recovery group-control group (n = 6) and muscle glycogen recovery-Nana Tabuchi Participant Powder Group (n = 6) administer glucose after muscle glycogen lowering treatment, 90 minutes later muscle glycogen The amount of muscle glycogen recovery-control group and muscle glycogen recovery-Tasanajin Participant's powder group was confirmed by confirming the muscle glycogen accumulation promoting effect by measuring the amount.
FIG. 1 summarizes the experimental methods of the initial value group, the muscle glycogen lowering group, and the muscle glycogen recovery group.

〔Experimental device〕
Kyodai Matsumoto type momentum measuring water tank Ishihara model (90cm x 45cm x 45cm, manufactured by Anitec Co., Ltd.) was used. The water depth was 34.2 cm, and the water temperature was 34.0 ° C. at which the mouse was able to maximize its swimming ability. Before the exercise experiment, the surface water flow of each lane was measured using a digital flow meter counter to confirm that the amount of water flow was constant.

-Swimming exercise for lowering muscle glycogen-
Swimming was performed for 10 minutes under running water of 10 L / min, and then the water flow rate was increased by 1 L / min every 5 minutes, and swimming was terminated when the water stayed in water for 7 seconds.

-Assessment of muscle glycogen accumulation-
-Initial value group: Hifuku muscle removal 4 hours after the start of light period-Muscle glycogen-reduced group: Hifuku muscle removal immediately after swimming-Muscle glycogen recovery group: 1.0 g / kg-wt glucose solution was intraperitoneally administered immediately after swimming, 90 After standing for a minute, excision of hyfuku muscle

The amount of glycogen in the collected hyfuku muscle was measured, and the amount of muscle glycogen was calculated. The amount of glycogen was measured according to the method of Xu et al. (J. Cell Mol. Med., 942-954, 2008).
For the control group, muscle glycogen recovery-the difference between the control group and the muscle glycogen reduction-control group, and for the Tanabata Participant Engineering powder group, the muscle glycogen recovery-Tabanachi Participant Engineering powder group and muscle glycogen reduction-Tanashi Ginseng The difference from the processed powder group was taken as the amount of muscle glycogen synthesis.
By comparing the amount of muscle glycogen synthesis in the control group and the Nanata Participant's powder group, muscle glycogen accumulation was promoted.

〔result〕
The amount of muscle glycogen at each timing of the initial value group, muscle glycogen reduction group, and muscle glycogen recovery group was measured. The results are shown in FIG.

図２及び表２の結果から、初期値群に比べて筋肉グリコーゲン量がコントロール群では６６％、田七人参加工粉末群では５８％へ減少し、遊泳運動を行ったことによる筋肉グリコーゲン量低下が達成できていたと考えられた。減少した筋肉グリコーゲンはグルコース溶液投与９０分間後で回復し、コントロール群で２５．８３μｍｏｌ／ｇ、田七人参加工粉末群で３６．２７μｍｏｌ／ｇとなった。

From the results of FIG. 2 and Table 2, the amount of muscle glycogen decreased to 66% in the control group and to 58% in the Tanachi Participant Powder Group compared to the initial value group, and the muscle glycogen amount decreased due to the swimming exercise. It was thought that it was achieved. The decreased muscle glycogen recovered 90 minutes after administration of the glucose solution, and it was 25.83 μmol / g in the control group and 36.27 μmol / g in the Nanatsuta Participant Powder Group.

<Acceleration of accumulation of muscle glycogen>
From the above results, the amount of muscle glycogen synthesized by administration of the glucose solution is the difference between the amount of muscle glycogen when the muscle glycogen is recovered and the amount of muscle glycogen when the muscle glycogen is lowered, and can be obtained by the following calculation.
Control group (μmol / g): 25.83-17.13 = 8.7
Nana Tanahi Participating Industrial Powder Group (μmol / g): 36.27-14.75 = 21.52
From the above calculation, the amount of muscle glycogen synthesis was 8.7 μmol / g in the control group and 21.5 μmol / g in the Tanashi Ninjin Participant Powder Group.
Therefore, the amount of muscle glycogen synthesis by ingesting Tanada Participant's powder increased 2.5 times (21.52 ÷ 8.7 = 2.5) compared to the control group. It was confirmed that the powder had a significant muscle glycogen accumulation promoting effect.

As an aspect of this invention, the following are mentioned, for example.
<1> A muscle glycogen accumulation promoter characterized by containing a processed powder of a genus Uchigiaceae plant.
<2> Processed powder of the genus Uchigiaceae plant is obtained by applying a strong acid aqueous solution having a concentration of 0.01 mol / L to 4 mol / L to the Uchigiaceae plant, and hydrolyzing it in the presence of a lower alcohol. The muscle glycogen accumulation promoter according to <1>.
<3> The muscular glycogen accumulation promoter according to any one of <1> to <2>, wherein the plant belonging to the genus Tochibaninjin is Ginseng.
<4> A food or drink comprising the muscle glycogen accumulation promoter according to any one of <1> to <3>.

  The muscle glycogen accumulation promoter of the present invention can increase the amount of muscle glycogen accumulation safely and easily without injury and without applying an exercise load. Furthermore, since the said muscle glycogen accumulation promoter is a natural product type and highly safe and easily available, it can be suitably used as a food or drink.

Claims (3)

  A muscle glycogen accumulation promoter characterized by containing processed powder of the genus Araceae plant.   The processed powder of the genus Uchigiaceae is obtained by subjecting the Uchigiaceae genus Tochibanin ginseng plant to a strong acid aqueous solution having a concentration of 0.01 mol / L to 4 mol / L and subjecting it to a hydrolysis treatment in the presence of a lower alcohol. The muscle glycogen accumulation promoter described in 1.   The muscle glycogen accumulation promoter according to any one of claims 1 to 2, wherein the plant belonging to the genus Uchigiaceae is a ginseng.

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