JP2015008663A - Homogenizing bag - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a novel homogenizing bag that enables more effective homogenizing treatment even for an analyte having difficulty in the homogenizing treatment such as a fibrous analyte or a meat among tissue pieces of living bodies, solid foods and the like.SOLUTION: Provided is a homogenizing bag 100 that comprises: a bag 50 comprising an opening 52 or opening portion; granular members 1; a temporary housing member 2 for temporarily housing the granular members 1; and a temporary housing portion 21 formed by the temporary housing member 2 and provided inside or outside the bag 50. The homogenizing bag 100 has a filter so as not to pass the granular members 1 and the analyte sample 5 put in the bag 50 or the analyte sample 5 having undergone the homogenizing treatment.

Description

  The present invention relates to a bag (homogenization bag) used for a process (homogenization process) for extracting microorganisms and the like from a specimen sample into a solution and homogenizing the extracted microorganisms and the like in the solution.

  Biological tissue pieces and solid foods (hereinafter also referred to as “foods”) may contain microorganisms such as bacteria, and the presence and type of such microorganisms are examined by inspection (microbiological examination). . In the examination of microorganisms, usually, microorganisms are extracted from a sample sample such as food to be examined into a solution (for example, diluted water), and the extracted microorganisms are homogenized in the solution (hereinafter referred to as “homogenization treatment”). )It is carried out. This homogenization process is a pre-stage process of the microorganism test, and is performed in order to accurately test the microorganism. Microorganisms are extracted by crushing or crushing a bag containing a specimen sample and a solution (for example, diluted water) (this bag is referred to as a “homogenized bag”). The extracted microorganisms are homogenized in the solution, the solution is taken out, and the microorganisms are inspected by an inspection device.

  Various types of homogenized bags have been proposed. For example, in Patent Document 1, a small space portion is provided in an internal space, and the liquid in the small space portion is liquid-tightly sealed over the entire width of the small space portion at an arbitrary height position. A homogenized bag in which a liquid reservoir is formed in the upper part of the tight seal has been proposed. This homogenized bag is said to be able to inhale the suspension easily even if it is a short pipette by inserting it vertically from the opening.

  Further, in Patent Document 2, in the homogenized bag with a filter, the filter has one side edge extending in the longitudinal direction adhered to one inner side surface of the bag body at a substantially center in the width direction of the bag body, and the other side. There has been proposed a homogenized bag in which the longitudinal side edges are integrally bonded in a state of being sandwiched by one side edge portion of both side edges extending in the longitudinal direction of the bag body. This homogenization bag is said to be able to ensure a sufficient capacity in the sample container and to smoothly perform operations such as injection of food samples and pipetting.

  The technique of Patent Document 3 is different from the homogenized bag of Patent Documents 1 and 2, but relates to a crushing kit used for extracting proteins, nucleic acids, and the like. This crushing kit has an opening and a bag for accommodating a sample, a plurality of hard granular members provided in the bag for crushing the sample by pressing the bag against the sample, and for opening and closing the opening. It is said that the structure piece and the operation are simple, and the tissue piece can be crushed quickly and efficiently.

JP 2001-346571 A JP 2002-82108 A JP 2006-230279 A

  Among biological tissue pieces and solid foods, fiber samples and elastic samples such as meat, squid and octopus are homogenized using the homogenization bags proposed in Patent Documents 1 and 2. Even so, these specimens cannot be sufficiently crushed or finely crushed, and microorganisms may not be sufficiently extracted. Therefore, a long time homogenization process may be required.

  Further, the crushing kit proposed in Patent Document 3 is a hard granular member fixed to a bag. Therefore, the crushing kit is considered to be able to crush foods etc. to a certain extent when applied to homogenization treatment, but the granular member fixed to the bag has no fluidity and is more effective homogenization Processing cannot be performed.

  The present invention has been made in order to solve the above-mentioned problems, and its purpose is to perform a homogenization treatment such as a fibrous specimen or an elastic specimen among living tissue pieces and solid foods. An object of the present invention is to provide a new homogenization bag capable of performing a more effective homogenization process even for a difficult specimen sample.

  A homogenization bag according to the present invention for solving the above-mentioned problems includes a bag having an opening or an opening, a granular member, and a temporary storage member for temporarily storing the granular member, and the temporary storage The temporary accommodation part which a member forms is provided in the inner side or the outer side of the said bag, It is characterized by the above-mentioned.

  The homogenization bag according to the present invention may have a filter for preventing passage of the granular member, the specimen sample put in the bag, and the specimen sample after the homogenization treatment.

  In the homogenization bag according to the present invention, the solution may be stored in advance.

  The homogenizing bag according to the present invention may have one or both of a temporary sealing means and an easy opening means.

  According to the homogenization bag of the present invention, even a specimen sample that is difficult to homogenize, such as a fibrous specimen or an elastic specimen, among living tissue pieces or solid foods, is more effective. Homogenization treatment can be performed.

They are a front view (A) which shows an example of the homogenization bag based on this invention, BB sectional drawing (B), and a front view (C) which shows the example which put the sample sample and the solution in the homogenization bag. Front view (A) showing a mode in which a temporary storage portion is provided at the inner corner of the homogenization bag, front view (B) showing a mode in which a temporary storage unit is provided at the inner bottom of the homogenization bag, and left and right sides of the homogenization bag It is a front view (C) which shows the aspect which provided the temporary accommodating part in the outer side of this side. It is the front view (A) and BB sectional drawing (B) which show an example which provided the filter in the homogenization bag. It is the front view (A) and BB sectional drawing (B) which show another example which provided the filter in the homogenization bag. It is the front view (A) and BB sectional drawing (B) which show an example which provided the check valve type filter in the homogenization bag. It is the front view (A) and BB sectional drawing (B) which show another example which provided the check valve type filter in the homogenization bag. Partial front view (A) showing an example provided with temporary sealing means, partial front view (B) showing another example provided with temporary sealing means, and an example provided with temporary sealing means and easy opening means It is a partial front view (C) shown. Partial front view (A) showing an example in which easy-opening means is provided in a homogenized bag sealed in all directions, and partial front view showing an example in which easy-open means and temporary sealing means are provided in a homogenized bag sealed in all directions (B).

  The homogenizing bag according to the present invention will be described in detail with reference to the drawings. In addition, this invention is not limited to the following embodiment, In the range of the summary, various deformation | transformation can be implemented.

[Homogenized bag]
The homogenization bag 100 (100a-100l) which concerns on this invention temporarily accommodates the bag 50 provided with the opening part 52 or the opening part 53, the granular member 1, and the granular member 1, as shown in FIGS. A temporary housing member 2 for the purpose.

  Since the homogenizing bag 100 has the temporary housing member 2 for temporarily housing the granular member 1, the portion containing the solution 4 of the homogenizing bag 100 containing the solution 4 or the granular member 1 is temporarily stored ( When a force is applied from the outside to a portion that is temporarily sealed (hereinafter also referred to as “temporary storage portion 21”), the temporary storage state (temporary sealing state) of the temporary storage portion 21 is released and stored. The granular member 1 spreads in the bag 50. When a force is applied from the outside to the homogenizing bag 100 containing the specimen sample 5 and the solution 4, the granular member 1 crushes, crushes, or finely crushes the specimen sample 5, and the microorganisms contained in the specimen sample 5 are contained. It can be extracted effectively. Furthermore, since the granular member 1 can move without being fixed to the inner wall, the extracted microorganisms can be effectively homogenized in the solution. As a result, even the specimen sample 5 that is difficult to homogenize can be subjected to more effective homogenization.

  Moreover, since the granular member is accommodated in the temporary accommodation part 10, when the specimen sample 5 is put in too much, the specimen sample 5 can be easily taken out before the temporary accommodation state is released.

  Furthermore, since the granular member 1 is temporarily accommodated in the homogenization bag 100, when the sample sample 5 is put in too much, the sample sample 5 can be easily taken out and the homogenization bag 100 is easily transported. As a result, handling of the homogenized bag 100 can be facilitated.

[Each component]
Hereinafter, each structure of the homogenization bag 100 will be described in detail.

(bag)
The bag 50 is filled with the specimen sample 5 and the solution 4 before the homogenization process and stores them. The bag 50 includes an opening 52 or an opening 53. The opening 52 may be provided in the bag 50 in advance as shown in FIG. 1 or the like, or may be provided by opening the opening 53 as shown in FIG. After the homogenization process, the contents such as the solution are taken out from the opening 52.

  As shown in FIG. 1 or the like, the opening 52 may be provided in advance in a bag 50 that is sealed on three sides, for example, or as shown in FIG. The opening part 53 may be provided, and the opening part 53 may be opened and provided. The opening 52 or the opening 53 may be provided at any position of the bag 50, but is usually provided on the upper side during the homogenization process. Although the number of the opening parts 52 and the opening parts 53 is one normally, two or more may be provided.

  The size of the opening 52 or the opening 53 is not particularly limited. In the case of the square bag 50 as shown in FIGS. 1 and 8, all of the upper sides may be the opening 52 or the opening 53. In addition, a part of the upper side may be the opening 52 or the opening part 53. Moreover, the shape of the opening part 52 or the opening part 53 is not specifically limited, either a linear shape as shown in FIG. 1 etc. may be sufficient, and a curve shape may be sufficient. Further, the position of the opening 52 or the opening 53 is not particularly limited, and may be provided on the upper side as shown in FIG. 1 or the like, may be provided on the side on the side, It may be provided on one or both of the front and rear surfaces.

  The bag 50 provided with the opening part 53 can hold | maintain, with the inside of the bag 50 sealed, as shown in FIG. Therefore, contamination of the bag 50 can be prevented until the opening portion 53 is opened, and inspection accuracy such as microbial inspection can be improved.

  The opening portion 53 is provided with easy-opening means 9 (9a, 9b) and can be easily opened by the easy-opening means 9. Details of the easy-opening means 9 will be described later. Further, the bag 50 may be provided with temporary sealing means 8 as shown in FIG. The temporary sealing means 8 can accommodate the specimen sample 5 and the solution 4 so as not to leak out after the specimen sample 5 and the solution 4 are accommodated, and details thereof will be described later.

  The portion other than the opening 52 is constituted by a sealed portion 3 that is sealed. The sealing part 3 should just have airtightness and intensity | strength of the grade which does not peel at the time of a homogenization process. The sealing part 3 can provide the film or sheet cut | disconnected by the predetermined magnitude | size with various sealing means. Such a sealing means may be a heat welding (heat sealing) method or a heat laminating method, or a means in which a film or sheet bending means and a heat welding method or a heat laminating method are used in combination. Such a sealing portion 3 is provided so as not to leak until the sample sample 5 and the solution 4 that have been charged are taken out after being homogenized.

  The bag 50 is flexible and has airtightness and strength such that they do not leak while the sample 5 and the solution 4 are homogenized. As such a bag 50, a resin film or sheet is preferably used. As the material of the resin film or sheet, for example, one or more selected from polystyrene, polyethylene, polypropylene, nylon, polyester, polycarbonate, synthetic rubber, and the like can be used. The resin film or sheet may have a single layer structure or a laminated structure in which the same or different materials are stacked. The resin film or sheet may be uniaxially stretched or biaxially stretched. The uniaxially stretched film or sheet is convenient because it can be easily opened by the easy opening means 9 described later. Although the thickness of the bag 50 is not specifically limited, The thing of the thickness within the range of 60 micrometers or more and 80 micrometers or less is used normally.

  The shape of the bag 50 can be appropriately selected depending on the mode of homogenization. For example, it may be a quadrangular shape as shown in FIG. 1 or the like, or may be a polygonal shape such as a triangular shape or a shape having a curved contour with reduced corner portions. Specific examples include bottom seal bags, side seal bags, pillow bags, gusset bags, standing pouches, and circular seal bags. The capacity of the bag 50 is not particularly limited, but is usually about 50 mL or more and 5000 mL or less.

(Temporary housing member)
The temporary storage member 2 is a member that temporarily stores (temporarily seals) the granular member 1 in the temporary storage portion 21. This temporary accommodation member 2 forms the temporary accommodation part 21 so that the granular member 1 will be in a temporary accommodation state (temporary sealing state). The temporary storage member 2 is provided such that the temporary storage portion 21 is formed inside or outside the bag 50.

  The temporary storage member 2 acts to ensure the temporary storage state of the temporary storage portion 21 until a force is applied from the outside. On the other hand, when a force is applied from the outside to the homogenization bag 100 containing the solution 4 or the sample sample 5 and the homogenization bag 100 containing the solution 4, the temporary storage member 2 changes the temporary storage state of the temporary storage unit 21. It releases and acts so that it can contact with the sample 5. The temporary storage state of the temporary storage unit 21 may be canceled before the sample sample 5 and the solution 4 are added, or may be canceled before the sample sample 5 or the solution 4 is added. It may be canceled when both of are included.

  Since the kind of the temporary housing member 2 can be the same as that of the temporary sealing means 8 (8a, 8b) to be described later, the description thereof is omitted here.

  The temporary storage member 2 may be provided alone in the homogenization bag 100, or may be provided in two or more. When providing two or more, the temporary accommodation member 2 may provide two or more of the same types, and may provide a combination of different types. When two or more of the same type are provided, for example, as shown in FIG. 2 (C), the temporary storage members 21 are provided on the outside of the bag 50 to form the temporary storage portions 21. The member 2 can be the same type. On the other hand, in the case of providing a combination of different types (not shown), for example, the temporary storage portion 21 may be provided on both the outer side and the inner side (inside) of the bag 50. The temporary storage member 2 forming the portion 21 and the temporary storage member 2 forming the inner temporary storage portion 21 can be made different types. Further, the number, size, and the like of the temporary storage member 2 may be appropriately adjusted according to the type of the specimen sample 5 or the number of granular members 1 to be stored.

  The temporary housing portion 21 is a space portion for temporarily housing (temporarily sealing) the granular member 1. The temporary storage portion 21 is formed on the outer side (outside) or the inner side (inside) of the bag 50 so that the temporary storage member 2 ensures a temporary storage state (temporary sealing state). The temporary accommodation portion 21 secures a temporary accommodation (temporary sealing) state until an external force is applied, and when the force is applied from the outside, the temporary accommodation state (temporary sealing state) is released.

  What is necessary is just to provide the position of the temporary accommodating part 21 according to the position of the force applied from the exterior. For example, when a homogenizer (also referred to as “stomacker”) is used, a force is applied to the homogenizing bag 100 when a movable paddle or roller of the homogenizer contacts the homogenizing bag 100. Therefore, the temporary storage unit 21 may be provided so as to have a height approximately equal to the height of the paddle or roller of the homogenizer when the homogenizer bag 100 is attached to the homogenizer.

  For example, as shown in FIGS. 1 and 2A and 2B, the temporary housing portion 21 (21a to 21) may be provided on the inner side (inside, inner wall) of the bag 50, or FIG. ), It may be provided on the outer side (outside, outer wall) of the bag 50. Such an aspect of the temporary housing portion 21 will be described in detail with reference to FIGS. 1 and 2. Note that, in the homogenized bag 100 sealed on the three sides with the sealing portion 3, among the three sides sealed, the side facing the opening is referred to as the “bottom side”, and the left side of the bottom side is referred to as the “left side”. Okay, the right side of the bottom is called the “right side”.

  The temporary storage portion 21 a shown in FIGS. 1A and 1B is an aspect provided inside the bag 50. The temporary housing portion 21a is a space portion (first temporary housing portion 21a) surrounded by two inner walls 51, 51. The first temporary storage portion 21a includes the entire bottom side of the bag 50, the temporary storage member 2 provided on the upper side of the bag 50 so as to face the bottom side, a lower part of the left side of the bag 50, and the lower side of the right side of the bag 50. The circumference is in contact with some four. A large number of granular members 1 are temporarily accommodated in the space portion (first temporary accommodation portion 21a).

  As shown in FIGS. 1 (A) and 1 (B), the first temporary housing portion 21a is provided between the opposing inner walls 51 and 51 so as to be parallel to the bottom side from the left side to the right side. Is provided. When the first temporary storage unit 21a applies a force from the outside to the homogenization bag 100 containing the solution 4, the temporary storage state of the temporary storage unit 21 is released, as shown in FIG. The granular member 1 temporarily accommodated in the temporary accommodation portion 21 a spreads in the solution 4. As a result, the expanded granular member 1 can crush, crush or crush the specimen sample 5 finely. The principle of releasing the temporary storage state of the temporary storage portion 21a is, for example, when a force is applied to the portion containing the solution 4 when a force is applied to the homogenization bag 100 containing the solution 4 from the outside. 21a is pressed. At this time, since the internal pressure of the temporary storage portion 21a is increased, the temporary storage state of the temporary storage member 2 is released by the internal pressure, and the temporary storage member 2 is opened.

  The temporary storage portion 21b shown in FIG. 2A is also provided inside the bag 50, and is a space portion (second temporary storage portion 21b) surrounded by the two inner walls 51, 51. The second temporary storage portion 21b includes a part of the left side of the bottom side of the bag 50, a temporary storage member 2 provided on the upper side of the part on the left side of the bottom side, a part of the lower side of the left side of the bag 50, The periphery is in contact with four of the temporary accommodation members 2 provided to face the lower part of the left side and have the same length. A large number of granular members 1 are temporarily accommodated in this space portion (second temporary accommodation portion 21b).

  The second temporary storage portion 21b is provided with the temporary storage member 2 from the left side to a desired position so as to be parallel to the bottom side between the opposing inner walls 51, 51. Further, the temporary storage member 2 is provided. The temporary storage member 2 is provided on the bottom side facing the left side from the desired position. When the second temporary storage unit 21b applies a force from the outside to the homogenizing bag 100 containing the solution 4, the temporary storage state of the temporary storage unit 21b is released, and the case shown in FIG. Similarly, the granular member 1 temporarily accommodated in the temporary accommodation portion 21 b spreads in the solution 4. As a result, the expanded granular member 1 can crush, crush or crush the specimen sample 5 finely.

  2A shows an example in which the adhesive tape 2a is used as the temporary housing member 2, a chuck 2b as shown in FIG. 2B may be used. In FIG. 2A, the temporary storage portion 21b is provided on the left side, but the temporary storage portion may be provided on the right side. In addition, the temporary storage portion may be provided so as not to contact the bottom side, or the temporary storage portion may be provided in a central region away from both sides so as not to contact the left side and the right side. Furthermore, two or more such temporary housing units may be provided.

  The temporary storage portion 21c shown in FIG. 2 (B) is also provided inside the bag 50. The difference from the temporary storage portion 21a of FIG. 1 (A) is that a chuck 2b is used as the temporary storage member 2. It is a point. The third temporary accommodation portion 21c is the same as the first temporary accommodation portion 21a shown in FIGS. 1A and 1B except for this point.

  The temporary storage portion 21 d shown in FIG. 2C is an aspect provided outside the bag 50. The temporary storage portion 21d is provided in the bag 50 with a separately prepared external bag (two external bags in the figure) as a space portion (fourth temporary storage portion 21d). In the fourth temporary housing portion 21d, the attachment location of the external bag is a temporary housing member 2c. When a force is applied from the outside to the external bag constituting the fourth temporary storage portion 21d, the temporary storage state of the fourth temporary storage portion 21d is released, and the granular member 1 temporarily stored therein is released. It enters the bag 50 and spreads in the solution. As a result, the expanded granular member 1 can crush, crush or crush the specimen sample 5 finely.

  2C illustrates an example in which the adhesive tape 2c is used as the temporary housing member 2, a chuck 2b as illustrated in FIG. 2B may be used. In FIG. 2C, the temporary storage portion 21d is provided on both the outer side of the left side and the outer side of the right side of the bag 50, but may be provided in only one of them.

  The temporary accommodating part 21 demonstrated above may be provided independently, and may be provided two or more. When providing two or more temporary accommodating parts 21, you may accommodate the granular member 1 of the same average particle diameter and the same material, respectively, and you may accommodate the granular member 1 of a different average particle diameter and material.

(Granular material)
As shown in FIGS. 1 to 6, the granular member 1 is temporarily accommodated inside or outside the bag 50. Specifically, it is temporarily accommodated in contact with the inner wall 51 inside the bag 50 or the outer wall 54 outside the bag 50. When a force is applied from the outside, the granular member 1 is released from the temporary accommodation state of the temporary accommodation member 2 and spreads into the bag 50 so that it can come into contact with the sample 5. The granular member 1 acts so as to crush, crush, or crush the specimen sample 5 during the homogenization process. Further, the granular member 1 can move freely by flowing in the bag 50. Therefore, a liquid flow such as convection occurs due to the flow of the granular member 1, and the test object such as the extracted microorganisms can be uniformly dispersed in the solution 4, and an effective homogenization process can be performed. These points are different from the conventional example in which the granular member 1 cannot freely flow.

  As the granular member 1, various members having such a hardness that can perform the above-described action during the homogenization treatment can be used, and the kind thereof is not particularly limited. The granular member 1 is preferably made of a material that does not adsorb microorganisms, fine specimen samples 5, food components, and the like, and can perform inspection with high inspection accuracy. Examples of the material of the granular member 1 include various ceramic particles such as silicon oxide particles, silicon carbide particles, zirconium oxide particles, titanium oxide particles, aluminum oxide particles, and glass particles, and metal particles such as steel particles and stainless steel particles. Can be used. Various treatments may be performed on the surface of the granular member 1.

  The shape of the granular member 1 is not particularly limited, but a rounded spherical or elliptical shape is preferable so as not to damage the bag 50, the temporary storage member 2, and other members described later. Since the granular member 1 with corners can quickly crush or finely crush the specimen 5, even if it is used as long as the granular member 1 has a shape that does not cause defects such as scratching. Good.

  Although the magnitude | size of the granular member 1 is not specifically limited, Usually, a thing with an average particle diameter of 1 mm or more and 5 mm or less can be used preferably. The “average particle size” in the present invention is a value obtained by averaging the measured particle size of the granular member 1 when the granular member 1 having 100 samples is measured with an optical microscope.

  The material, shape, and average particle diameter of the granular member 1 can be selected according to the type of the specimen sample 5. At that time, the number of granular members 1 to be accommodated in the temporary accommodating portion 21 can also be appropriately selected. Further, as shown in FIG. 2C, in accordance with the specimen sample 5, the granular members 1 having different average particle diameters may be used in combination, or the granular members 1 made of different materials may be used in combination. Also good.

(Other parts)
In addition to the bag 50, the granular member 1 and the temporary housing member 2, the homogenizing bag 100 can be provided with other members as necessary. Examples of other members include the filter 7 shown in FIGS. 3 to 6, the temporary sealing means 8 and the easy-opening means 9 shown in FIGS. 7 and 8.

  The filter 7 (7a, 7b, 7c, 7d) is provided to prevent the granular member 1 and the specimen sample 5 from passing therethrough. Such specimen sample 5 is both before and after the homogenization treatment. The specimen sample 5 after the homogenization treatment is crushed, crushed or finely crushed, and the filter 7 can prevent the specimen sample 5 from passing through. For example, as shown in FIGS. 3 to 6, the filter 7 is provided in various forms.

  The filter 7a shown in FIGS. 3A and 3B is provided between the opposed inner walls 51 and 51 so as to bisect the interior of the bag 50. FIG. Such a structure is formed by joining both ends of the filter 7 a to the left and right sealing portions 3, 3 of the bag 50. Of the spaces 71 and 72 divided approximately by the filter 7a, the sample 5 is put into the space 71 on the side where the temporary storage unit 21 is provided. The input specimen sample 5 does not pass to the side of the space 72 due to the presence of the filter 7a even after being homogenized by the granular member 1. On the other hand, the specimen sample 5 is not put into the space 72 on the side where the temporary storage unit 21 is not provided. In this space 72, only a solution in which microorganisms extracted by the homogenization process are uniformly dispersed can exist. As a result, a solution in which only microorganisms and the like are uniformly dispersed can be taken out and inspected with a pipette or the like.

  The filter 7b shown in FIGS. 4A and 4B is provided in a part of the inside of the bag 50 so as to bisect the inside of the bag 50. Such a structure is formed as a folded filter by joining both ends of the filter 7 to the sealing portion 3 on the left side of the bag 50. Of the spaces 71 and 72 divided by the filter 7, the specimen sample 5 is put into the space 71 on the side where the temporary storage unit 21 is provided. The input specimen sample 5 does not pass through the space 72 due to the presence of the filter 7 even after being homogenized by the granular member 1. On the other hand, the specimen sample 5 is not put into the space 72 on the side where the temporary storage unit 21 is not provided. In this space 72, only a solution in which microorganisms extracted by the homogenization process are uniformly dispersed can exist. As a result, a solution in which only microorganisms and the like are uniformly dispersed can be taken out and inspected with a pipette or the like.

  The filter 7c shown in FIGS. 5A and 5B is provided between the opposed inner walls 51 and 51 so as to bisect the inside of the bag 50 in the vertical direction. Such a structure can be formed using two substantially S-shaped (or step-shaped) filters 7c as shown in FIG. In this example, one end of each filter 7c, 7c is joined to the same height of the opposing inner walls 51, 51, and the other end of each filter 7c, 7c is intermediate between the opposing inner walls 51, 51. It is in contact. Unlike the above-described filters 7a and 7c, the contact portion of the filter 7c functions as a check valve. Of the upper and lower spaces 71, 72 divided by the filter 7c, the lower sample space 71 where the temporary storage unit 21 is provided retains the sample sample 5, and the upper sample space 72 does not retain the sample sample 5. That is, the filter 7c passes the specimen sample 5 from the upper space 72 side to the lower space 71 side, but passes the specimen sample 5 from the lower space 71 side to the upper space 72 side. I won't let you. The filter 7c does not allow the specimen sample 5 to pass through the granular member 1 from the lower space 71 side to the upper space 72 side, similarly to the specimen sample 5. In this space 72, only the solution 4 in which the microorganisms extracted by the homogenization process are uniformly dispersed can exist. As a result, the solution 4 in which only microorganisms and the like are uniformly dispersed can be taken out and inspected with a pipette or the like.

  The filter 7d shown in FIGS. 6A and 6B is provided between the opposed inner walls 51 and 51 so as to bisect the interior of the bag 50 in the same manner as in FIGS. 5A and 5B. Yes. Such a structure can be formed by using one substantially S-shaped (or stepped) filter 7d as shown in FIG. In this example, one end of the filter 7d is joined to one inner wall 51, and the other end is brought into contact with the other inner wall. As with the filter 7c, the contact portion of the filter 7d functions like a check valve. Of the upper and lower spaces 71 and 72 divided into two by the filter 7d, the lower sample 71 where the temporary storage unit 21 is provided retains the sample sample 5, and the upper sample space 72 does not retain the sample sample 5. That is, the filter 7c allows the specimen sample 5 to pass from the upper space 72 side to the lower space 71 side, but does not allow the specimen sample 5 to pass from the lower space 71 side to the upper space 72 side. . The filter 7 c does not allow the sample member 5 to pass through the granular member 1 from the lower space 71 side to the upper space 72 side, similarly to the sample sample 5. In this space 72, only the solution 4 in which the microorganisms extracted by the homogenization process are uniformly dispersed can exist. As a result, the solution 4 in which only microorganisms and the like are uniformly dispersed can be taken out and inspected with a pipette or the like.

  As described above, the filters 7c and 7d do not allow the granular member 1 and the specimen sample 5 to pass from the lower space 71 side to the upper space 72 side. The specimen sample 5 is densely present. Therefore, the granular substance 1 can crush, crush or crush the specimen sample 5 finely. As a result, the homogenization bag 100 provided with the filter 7c or 7d can be more effectively homogenized.

  The filter 7 only needs to have a pore size that does not allow the particulate member 1 and the specimen sample 5 after being homogenized to pass through. The pore diameter is arbitrarily selected according to the type of the specimen sample 5, and examples thereof include those of 10 μm or more and 20 μm or less. The material of the filter 7 is not limited, and examples thereof include polyolefin such as low density polyethylene. Further, the structure form of the filter 7 is not particularly limited, and examples thereof include various structure forms such as a nonwoven fabric, a net, and a porous. More specifically, a microporous film made of low density polyethylene or the like can be used.

  The homogenization bag 100 provided with the filter 7 does not need to filter the solution 4 after the homogenization treatment, and can use the solution 4 after the treatment as it is for culturing or microbial inspection. In addition, the homogenized bag 100 can reduce contamination of contaminants that are likely to occur after filtration.

  The temporary sealing means (temporary sealing portion) 8 is a means for temporarily sealing the opening 52 as shown in FIG. The temporary sealing means 8 can be provided in the bag 50 which has the opening part 52, for example. Further, the temporary sealing means 8 may be capable of previously sealing the opening 52, or after releasing the sealing state of the previously sealed opening 52, the opening 52 is again formed. It may be one that can be sealed.

  As the temporary sealing means 8, for example, a chuck 8a as shown in FIG. 7A or an adhesive tape 8b as shown in FIGS. 7B and 7C may be used. These temporary sealing means 8 may be provided alone or in combination of two or more.

  Moreover, the temporary sealing means 8 may be provided in the bag 50 which does not have the opening part 52, as shown to FIG. 8 (B). The provision of the temporary sealing means 8 in this manner is convenient because the opening after being opened by the easy opening means 9 can be temporarily sealed again.

  The easy-opening means (easy-opening part) 9 is means for easily opening the homogenized bag 100 as shown in FIG. The easy-opening means 9 can be removed together with the temporary sealing means 8 when the temporary sealing means 8 is provided in the vicinity of the opening of the homogenization bag 100 as shown in FIG. Can be provided in position. For example, as shown to FIG. 8 (A) (B), it can provide in the position which can remove the sealing part 3 of the homogenization bag 100, and can provide the opening part 52. FIG.

  As the easy-opening means 9, for example, a notch (or also referred to as “notch”) 9 a as shown in FIGS. 7C, 8 </ b> A, and 8 </ b> B, FIG. 7C and FIG. ) A perforation as shown in (B) or a half cut 9b may be used. These easy-opening means 9 may be provided alone or in combination of two or more.

  When one or both of the temporary sealing means 8 and the easy opening means 9 are provided, (i) for example, when the temporary sealing means 8 is provided in the opening 52, the homogenized bag until the temporarily sealed state is released Contaminants are mixed in until the homogenization process is performed by preventing contamination from entering 100 or releasing the temporary sealing state and putting the specimen sample and solution into the temporary sealing state again. Can be prevented. (Ii) Further, for example, when the easy-opening means 9 is provided, the opening 52 can be provided in the all-sealed homogenization bag 100 without the opening 52, and the specimen sample 5 and Solution 4 can be added. (Iii) Moreover, when the temporary sealing means 8 and the easy-opening means 9 are provided, the temporary sealing means 8 after removing the temporary sealing state of an aspect mentioned above is removed by the easy-opening means 9 In addition, it is possible to prevent contamination from entering the homogenizing bag 100 until the temporary sealing state as described above is released after the opening 52 is provided in the all-sealed homogenizing bag 100 without the opening 52. Then, the temporary sealing state is canceled and the specimen sample 5 and the solution 4 are added, and then the temporary sealing state is set again, thereby preventing contamination by contaminants before the homogenization treatment. .

  Moreover, the form which accommodated the solution 4 previously may be sufficient as the homogenization bag 100 which concerns on this invention. The homogenization bag 100 that contains the solution 4 in advance only needs to put the specimen sample 5 in the homogenization process, and can improve workability. In addition, contamination of contaminants that can easily occur when the solution 4 is added can be reduced.

[Method for producing homogenized bag]
The homogenizing bag 100 according to the present invention can be manufactured by applying various means. For example, the homogenized bag 100 can be manufactured using a three-sided bag as shown in FIGS. The three-sided bag is a bag in which three sides (the bottom side and the left and right sides) of a rectangular bag are sealed.

  The homogenizing bag 100 using a three-sided bag is a process in which a plastic film is overlaid on another plastic film and the ends thereof are bonded together (bonding process), and the granular member 1 is put into the bonded plastic film. A step of providing a temporary accommodation member 21 by providing the temporary accommodation member 2 on the plastic film in which the granular member 1 is introduced and bonding the portion where the temporary accommodation member 2 is provided (temporary accommodation portion) Forming step). In addition, you may provide other processes, such as a sterilization process process, as needed.

(Lamination process)
The bonding process is a process in which two plastic films of the same size are overlapped and three end portions are bonded to form a bag shape. In this bonding step, a total of three end portions of one bottom portion and two side surface portions of the two overlapped plastic films can be bonded together to form a bag shape. The “bottom part” as used herein is one of two end parts that are generally short in length among the four end parts of the two overlapped plastic films. . In addition, the “side surface portion” referred to here is one of two end portions that are generally long in length among the four end portions of the two overlapped plastic films. is there. “Two side portions” are both of two end portions that are generally long in length among the four end portions of the two overlapped plastic films.

  The means for attaching the two plastic films is not particularly limited as long as the attached plastic film has hermeticity and strength so as not to be peeled off during the homogenization treatment, and various methods can be applied. For example, a heat laminating method or a heat welding (heat sealing) method can be preferably applied.

(Input process)
The charging process is a process of charging the granular member 1 into the bonded plastic film. The granular member 1 can be thrown in from the end part (opening part) which is not bonded among the bonded plastic films.

(Temporary housing part forming step)
The temporary storage portion forming step is a step of providing a temporary storage portion 21 that stores the granular member 1 by providing the temporary storage member 2 on a plastic film and bonding the portions where the temporary storage member 2 is provided. The temporary storage member 2 may be provided in accordance with the shape of the temporary storage portion 21. When the adhesive tape 2a is used as the temporary housing member 2, the temporary housing portion 21 can be provided by pasting the adhesive tape 2a on the inner surface of one plastic film and then pasting it on the other plastic film. . As means for attaching the adhesive tape 2a, for example, means such as pressure bonding can be applied. In the case of the chuck 2b as the temporary storage member 2, the chuck 2b corresponding to the inner surfaces of both plastic films is provided by applying means such as heat welding (heat sealing), and then the chucks 2b are overlapped and temporarily stored. A portion 21 can be provided. Other methods may be used.

(Other processes)
Examples of other processes include a sterilization process. The sterilization process is a process of sterilizing two bonded plastic films, and the internal space of the homogenized bag 100 is aseptic by this process. The sterilization treatment is usually performed by irradiation with gamma rays or the use of ethylene oxide gas. This sterilization step can be omitted if all members such as the plastic film, the granular member 1 and the housing member 2 are sterilized with gamma rays or the like.

  The filter 7 may be provided at the same time as the bag 50 is formed, or may be provided after the bag 50 is formed. The means for bonding can be provided by a known means such as a heat sealing method or a heat laminating method. Further, the opening portion 8 may be provided before the bag 50 is formed, or may be provided simultaneously with the formation of the bag 50.

[How to use homogenized bags]
The homogenization bag 100 according to the present invention is normally used for a homogenization process after the specimen sample 5 and the solution 4 are introduced from the opening 52. The types and ratios of the specimen sample 5 and the solution 4 to be input are arbitrarily selected and determined.

  The solution 4 is appropriately set according to the specimen sample 5 and the type of microorganism to be measured for the microorganism test. For example, a buffer solution such as physiological saline or phosphate buffer can be used as a diluent. The solution 4 is normally sterilized, and is poured from the opening 52 of the homogenization bag 100 together with the specimen sample 5 during the homogenization process. Further, the solution 4 may be sealed in a state where it is put in the homogenization bag 100 in advance. The solution 4 is introduced so that the specimen sample 5 can be diluted by 2 times to 100 times, for example, about 10 times.

  The specimen sample 5 is an object to be examined for microorganisms and is inserted from the opening 52 of the homogenization bag 100. Examples of the sample 5 include, but are not limited to, animal tissue, plant tissue, seafood, food, and the like. In particular, the homogenization bag 100 according to the present invention is an elastic sample such as a fibrous sample or a flexible sample such as meat, squid, octopus, or meat among the sample samples 5 described above. It can be suitably used for the specimen sample 5 that is difficult to crush or crush.

  The homogenization bag 100 into which the specimen sample 5 and the solution 4 are charged can be attached to a commercially available homogenizer and homogenized. In addition, the homogenization bag 100 according to the present invention can be homogenized using the pressing force of the hand.

  As described above, the homogenization bag 100 according to the present invention is such that when the external force is applied to the homogenization bag 100 containing the specimen sample 5 and the solution 4, the accommodated granular member 1 crushes or crushes the specimen sample 5. The microorganisms contained in the specimen sample 5 can be extracted effectively by crushing or finely crushing. Furthermore, since the granular member 1 can move freely in the bag 50, convection of the solution is promoted, and the extracted microorganisms can be effectively homogenized in the solution. As a result, even the specimen sample 5 that is difficult to homogenize can be subjected to more effective homogenization.

DESCRIPTION OF SYMBOLS 1 Granular member 2, 2a, 2b, 2c Temporary accommodation member 3 Sealing part 4 Solution 5 Specimen sample 6 Solution liquid surface 7, 7a, 7b, 7c, 7d Filter 8, 8a, 8b Temporary sealing means (temporary sealing Part)
9, 9a, 9b Easy opening means (easy opening part)
21, 21a to 21d Temporary container 50 bag 51 inner wall 52 opening 53 opening part 54 outer wall 100, 100a to 100l homogenized bag

Claims (4)

  It has a bag provided with an opening or an opening, a granular member, and a temporary storage member for temporarily storing the granular member, and the temporary storage portion formed by the temporary storage member is on the inside or the outside of the bag A homogenized bag characterized by being provided.   The homogenization bag according to claim 1, further comprising a filter for preventing passage of the granular member, the specimen sample to be put in the bag, and the specimen sample after the homogenization treatment.   The homogenization bag according to claim 1 or 2, which contains a solution in advance. The homogenization bag of any one of Claims 1-3 which has one or both of a temporary sealing means and an easy-opening means.

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