JPH02176462A - Method for picking up extract from specimen and specimen containing bag suitable for said method - Google Patents

Abstract

PURPOSE:To remove fat particle in emulsion and the particulates of crushed material by floating and settling low specific-gravity particles and high specific- gravity particles in the emulsion, and directly picking up extract from the central part. CONSTITUTION:A bag 10 which contains a specimen and diluting liquid is set in a homogenizer. A Pushing force is applied repeatedly with a pusher from the outside of the bag 10, and the specimen is sufficiently crushed. Emulsion of the crushed material is prepared. At this time, the pusher is not applied to a region to which an elastic pad 13 is fixed. The bag 10 is taken out of the homogenizer and placed quietly for a while. Low specific-gravity particles and high specific-gravity particles are floated and settled. The particles are extracted out of the elastic pad region which is located at the central part by using a stab pickup means such as, e.g. an injector. A hole which is formed by stabbing in the pad 13 is tightly closed again by the recovering force of the pad 13. The emulsion does not leak through the part.

Description

[Detailed Description of the Invention] Industrial Applicability The present invention is designed to store a sample such as food or biological tissue in a sample storage bag together with a sterilized diluent if necessary, and to repeat the process from outside the bag using a homogenizer. This invention relates to improvements in the method of sufficiently crushing a specimen by applying pressure, preparing an emulsion of the crushed specimen, and collecting the precepts as an extract, and the method, particularly regarding a specimen storage bag suitable for mechanized assay .

Problems with the conventional technology Conventionally, samples for microbial testing of food, biochemical, physiological, hygienic, medical, pathological testing of biological tissue, etc., are placed in a sample storage bag along with a diluent if necessary. A method of storing the sample, applying pressure from the outside of the bag with a homogenizer to sufficiently crush the sample, preparing an emulsion of the crushed sample, and extracting the emulsion, and a sample storage bag used in such a method. Are known.

The operation of this method will be described in detail in the case of testing the viable bacteria count of a specimen as follows.

■) Store the specimen in the specimen storage bag. At this time, a sterile diluent is usually added. Also, accurately measure the sample and diluent.

2) Set the sample storage bag containing the sample or the sample and the diluent in the homogenizer, and at this time press the opening of the sample storage bag so that the contents are not pushed out through the opening and move it back and forth while shifting the phase. Using a homogenizer with a plurality of buttons, the bag is repeatedly pressed from the outside to sufficiently crush the specimen, thereby preparing an emulsion of the crushed specimen.

3) Remove the sample storage bag from the homogenizer, and extract the extract by measuring the required amount from the opening using a suction means such as a pipette.

4) The extract is further diluted in several dilution series.
The extracts at each dilution rate are cultured and the number of colonies is counted.

The emulsion obtained by the above method contains fat particles,
There are many large and small pieces of crushed material, and if the purpose of the extract is to test for microorganisms, especially for bacterial count, it is difficult to distinguish these particles from microorganisms or their colonies. Therefore, it is desired to remove such foreign substances from the extract as much as possible.

For this purpose, there is also a specimen storage bag that divides the inside of the bag into two chambers using a mesh sheet, stores the specimen in one chamber, crushes it, and then extracts the emulsion from the other chamber. Are known. However, if the mesh is made too fine, not only will the strength of the mesh decrease, but there is a risk that bacteria will be trapped in the mesh. Therefore, there is a limit to how fine the mesh can be, and the amount of crushed particles that can be filtered through the mesh mixes into the extract. Furthermore, in the conventional method, since a suction means such as a pipette is inserted from above the liquid surface, it is inevitable that low specific gravity substances, particularly lipid particles, on the liquid surface adhere to the suction means. These make the colony coefficients mentioned above difficult.

Furthermore, all of the above operations, except for crushing the specimen, were performed manually. It is desired to mechanize and automate these operations.

Furthermore, if there is a possibility that the specimen contains pathogenic bacteria, it is desirable to minimize the opportunity for workers to come into direct contact with the emulsion or extract.

Therefore, the present inventor has conducted extensive research into a method for solving the above-mentioned problems and improvements in specimen storage bags etc. suitable for the method.
The present invention has now been completed.

Means for Solving the Problems The basic method of the present invention is to place a predetermined amount of specimen in a specimen storage bag.
If necessary, store the sample together with a predetermined amount of sterilized diluent, crush the sample by repeatedly applying pressure from outside the bag using a homogenizer, prepare an emulsion of the crushed sample, and add a portion of the solution. When collecting the emulsion with an extract, the low-specific gravity particles and high-specific gravity particles in the emulsion are floated and settled, and the extract is directly collected from the intermediate portion between them.

As a method for collecting the extract directly from the middle part of the emulsion, various methods can be used as described below.

For example, an elastic pad is fixed to one of the inner or outer surfaces of the facing sheet surface of the sample storage bag, and the low-specific gravity particles and high-specific gravity particles in the emulsion are floated or settled, and a syringe-shaped A method of collecting an extract using a puncture collection method.

An opening is provided on one of the inner or outer surfaces of the facing sheet surface of the sample storage bag, the opening is temporarily sealed, and the low-specific gravity particles and high-specific gravity particles in the emulsion are floated or settled, and the intermediate between them is A method of locating the aperture in a portion and opening the aperture at least partially to allow the extract to flow out of the aperture or to be collected by suitable collection means.

As a result, an extract from which almost all fat particles and crushed particles in the emulsion have been removed can be collected.

The outline of the method of the present invention has been described above, and the present invention will be further explained below through examples of the present invention with reference to the accompanying drawings. The above and further features and advantages of the invention will become clearer from the following description.

Example The basic method of the present invention is as follows.

■ Prepare a sterilized specimen storage bag.

■ Weigh the specified amounts of the specimen and sterile diluent and store them in the specimen storage bag.

■ Place the sample storage bag in the homogenizer, crush the sample, and prepare the emulsion.

■ Low specific gravity particles in the emulsion are floated, high specific gravity particles are allowed to settle, and the emulsion is separated into a floating layer, a settling layer, and an intermediate layer between them.

■ Collect the extract directly from the middle layer.

The sample storage bag IO is either sterilized in advance or sterilized prior to use. The sample storage bag may be a bag in which two rectangular sheets made of polyethylene or a laminate material of polyethylene and nylon are overlapped and heat-sealed on three sides of the rectangular shape. Such specimen storage bags are commercially available.

The sample storage bag can be sterilized using autoclave, ultraviolet rays, gamma rays,
This is done using ethylene oxide gas, etc.

There are various methods for directly collecting the extract from the intermediate layer, and an appropriate sample storage bag is used depending on the method. Therefore, examples of sample storage bags will be described below with reference to the drawings, and as necessary, methods for extract collection using these sample storage bags will be described.

FIG. 1 is a schematic perspective view of a specimen storage bag IO used in the method of the present invention. In this example, the specimen storage bag IO
A pad 13 made of an elastic material is fixed to the outer surface of one of the opposing sheets 11 and 12. The elastic pad may be fixed to the inner surface of the sheet 11. The elastic pad may be made of a plastic elastic material with good adhesion to the sheet material and may be fixed to the sheet material by an appropriate fixing method such as heat welding or adhesion. In this embodiment, the elastic pad 13 is fixed at a position in the middle of the depth that the emulsion of the crushed specimen occupies within the specimen storage bag 10.

For example, weigh a predetermined amount from a sample such as raw meat for ham,
It is stored in a specimen storage bag 1O together with a predetermined amount of diluent.

The specimen storage bag IO containing the specimen and diluent is set in a homogenizer (not shown), and the specimen is sufficiently crushed by a button that repeatedly applies pressing force from the outside of the specimen storage bag 10. Prepare the emulsion. At this time, the area to which the elastic pad is fixed is made to be out of the working surface of the bushing.

Take out the sample storage bag lO from the homogenizer and let it stand for a while to float the low specific gravity particles and high specific gravity particles, respectively.
They are allowed to settle and extracted from the elastic pad area located in their intermediate portion using a puncture collection means, for example a syringe. The puncture hole made in the elastic pad is sealed by the restoring force of the elastic pad, and the emulsion will not leak out from there.

FIG. 2a is a schematic perspective view showing a modification of the specimen storage bag IO shown in FIG. 1, and FIG. It is. As shown in FIG. 2a, the elastic pad 13 may be provided close to the opening 14 of the sample storage bag IO. In this case, it is preferable that the homogenizer is specially provided with a heat sealing mechanism so that when the sample storage bag 10 is set, the upper part of the elastic pad 13 is automatically sealed 15 in the lateral direction. After the emulsion is prepared, as shown in FIG. The storage bag 10 is tilted and left for a while to allow the low-specific gravity particles and high-specific gravity particles to float and settle, respectively, and extract liquid is collected from the elastic pad area using puncture collection means (not shown).

When performing the above method mechanically, one specimen storage bag IO is extracted from the storage section of the specimen storage bag by, for example, suction means.
The opening is opened by suctioning away from each other from the outside of the facing sheet surface of the opening, and a predetermined amount of the sample and, if necessary, a predetermined amount of sterilized diluent are introduced into the homogenizer. Transport the storage bag,
After setting and performing a crushing operation, the specimen storage bag is taken out from the homogenizer, and after being left on a rack for a while, for example, the puncture collection means is operated under robot control to collect the extract from the elastic pad area. When using the sample storage bag according to the embodiment shown in Fig. 2, the rack is tilted and the part to which the elastic pad is fixed is positioned at the middle level of the depth of the emulsion, and then left to stand still to separate the layers. Collect the extract. When collecting the extract, the sheet around the elastic pad area can be suction-supported by the suction means to avoid damage to the opposing sheet surface 12 by the puncture collection means. Additionally, the sheet on the opposing side can be suctioned away from the sheet provided with the elastic pad. These suction means can be provided, for example, on the above-mentioned rack. In the case of the specimen storage bag shown in FIG. 1, the specimen storage bag 10 after collection is heat-sealed at the opening of the specimen storage bag, and then opened into a second container.
In the case of the sample storage bag shown in the figure, just put it in a plastic bag or the like and dispose of it all together. If the contents may cause environmental pollution, the contents may be sterilized, the sample storage bag may be incinerated, or the sample storage bag may be sterilized and then disposed of.

The puncture collection means must be replaced between specimens or disposable to avoid contamination between different extracts.

FIG. 3 is a plan view of a sample storage bag IO according to another embodiment of the present invention. In this embodiment, the specimen storage bag 10
In this case, an extraction port 16 is provided by making an X-shaped cut in one side 11 of the opposing sheets, and the extraction port 16 is sealed with an adhesive sheet 17.

In the sample storage bag 10 of this embodiment, the upper part of the adhesive sheet 17 is attached to the second layer before or after preparing the emulsion of the crushed sample.
Heat-seal in the same manner as shown in the figure to float or settle the low-specific gravity particles and high-specific gravity particles of the emulsion, respectively, and then tilt the sample storage bag IO and position the extraction port 16 in the middle of them. At least a portion of the adhesive sheet is peeled off on the container that receives the extract, and the extract is allowed to flow out from the extraction port 16.

FIG. 4 is a plan view showing a modification of the embodiment shown in FIG. is fixed to. This prevents the opposing sheets of the sample storage bag from coming into close contact with each other, making it easier to collect the extract.

In the embodiment of FIGS. 3 and 4, the extraction port 16 is
When placing the sample storage bag 10 above the area occupied by the contents and setting it in the homogenizer, it is necessary to hold the sample storage bag 10 by pressing the lower part of the extraction port 16. This is because the compressed contents (
Specimen, diluent.

This is because there is a risk that air) may force the adhesive sheet and peel it off from the extraction port 16 and flow out. When the sample storage bag 10 according to these embodiments is used for mechanized processing, a "knob" that is pinched by the machine is used to peel off the adhesive sheet.
It is better to provide the portion on an adhesive sheet.

FIG. 5 is a plan view showing a modification intermediate between the embodiments of FIGS. 2 and 3. FIG. In this embodiment, the sample storage bag 1
0 has an elastic pad 13 on the inner surface of one side 11 of the facing sheet.
A notch 13a of a desired width is provided in the center of the region to which the elastic pad 13 is fixed over the entire thickness of the elastic pad and the sheet to which it is attached, and an adhesive sheet 17 is formed on the surface of the cut 13a. is attached.

In this example, the specimen storage bag 1O is tilted and left standing so that the elastic pad 13 is located between the floating layer and the sedimentation layer, and after the emulsion of the crushed specimen is separated into layers, the adhesive Peel off the sheet 17.

Thereafter, a suction collection means (not shown) such as a pipette is inserted through the cut portion 13a to collect the extract. After removing the pipette, the cut 13a is sealed by its own restoring force and no emulsion can escape. The purpose of the adhesive sheet 17 in this embodiment is to prevent the notch portion 13a from being contaminated during the storage period of the sample storage bag IO and to prevent leakage in the unlikely event.

Therefore, if there is no such danger, the adhesive sheet 17
is not necessary.

FIG. 6a is a plan view showing a modification of the embodiment shown in FIG. 5, and FIG. 6 is a schematic enlarged cross-sectional view taken along line BB in FIG. 6a. In this embodiment, an elastic pad 13 is fixed to the surface of one of the opposing sheets 11 of the sample storage bag 10. A pipette insertion guide recess 13b is provided in the center of the surface of the elastic pad 13, and a desired width is provided from the bottom of the guide recess 13b to the remaining thickness of the elastic pad and the entire thickness direction of the sheet 11. A notch 13a is made. The method of use is almost the same as the embodiment shown in FIG.

An advantage of this embodiment of FIG. 6 is that the pipette insertion guide recess 13b facilitates the insertion of a sampling means such as a pipette into the elastic pad 13 which can be sealed by restoring force. Therefore, extraction work can be carried out reliably both manually and mechanized.

The sampling means such as a pipette used in the sample storage bag IO according to the embodiment of FIG. 5.6 should be replaced for each sample or should be disposable. In addition, the specimen storage bag 10 according to the embodiment of FIG. 5.6
In this case, it is not necessary to make a cut in the sheet 11 in the area where the elastic pad is fixed. In this case, the tip of the pipette may be made sharp so that the pipette can break through the sheet 11 when collecting the extract.

FIG. 7a shows a sample storage bag 10 according to another embodiment of the present invention.
FIG. 7A is a schematic cross-sectional view taken along line BB in FIG. 7A. In this embodiment, the sample storage bag 1
A protrusion 19a extending laterally on one side 11 of the opposing sheet above the content level at 0, and a groove 19b on the other side of the sheet are provided, and when these are fitted together, the sample storage bag A seal structure 19 is formed that divides the interior of the sample storage area 10 into a sample storage area 21 and an extraction area 22 . Such a seal structure itself is a mystery. In the present invention, small holes or extraction ports 23 are formed at both ends of this seal structure. In use, after an emulsion of the crushed specimen is formed and the low-specific gravity particles and high-specific gravity particles float and settle, the specimen storage bag 10 is tilted and one of the small holes 23 is positioned in the middle of them for extraction. Let the liquid flow out from the extraction port.

At that time, the other extraction port 23 functions as an air introduction port,
It also has the advantage that the extract can be poured out even if the specimen storage bag IO is tilted in any direction. When there is only one extraction port, there is an effect of suppressing the outflow of the extract by reducing the internal pressure.

FIG. 8 is a plan view showing a modification of the embodiment shown in FIGS. 7a and 7b. In this embodiment, a second seal structure 24 is formed above the seal structure 19 having the extraction port 23 . The second seal structure 24 extends laterally over a desired dimension on both sides of the opening of the sample storage bag 10. As is now easily understood, these first
and the second sealing structure 19.24, the extraction area 22
A pocket-shaped extract reservoir 25 is formed in the sample storage bag 1.
When tilting the opening, it is possible to prevent the extract from overflowing from the opening.

In the embodiments of FIGS. 7 and 8, if the sheet material forming the specimen storage bag is not very flexible, the extraction area 2
2. There is a possibility that the facing sheets of the extract reservoir 25 portion remain in close contact with each other. In this case, the sample storage bag support structure in the rack can be appropriately deformed to separate the opposing sheets. Alternatively, a spacer member may be provided between the opposing sheets close to the seal structure.

When the sample storage bag according to the embodiment shown in FIG. 7 is used, the extract is received in a container such as a beaker and collected using a collecting means such as a pipette. When the sample storage bag according to the embodiment shown in FIG. 8 is used, samples can be collected directly from the extract reservoir 25.

FIG. 9 is a plan view showing still another modification of the embodiment shown in FIGS. 7 and 8. FIG. In this embodiment, the second seal structures 24 extend across the entire width of the specimen storage bag 10 in the lateral direction, forming an extraction liquid chamber 26 that is completely sealed from the outside of the specimen storage bag IO. are doing. This embodiment has the advantage that by collecting the extract from the middle portion of the emulsion transferred to the extract chamber 26, the low specific gravity particles and the high specific gravity particles can be separated in two stages.

Of course, the extract chamber 26 can be provided with the elastic pad or the elastic pad and adhesive sheet shown in FIGS. 1, 5, and 6.

FIG. 1Oa is a schematic plan view showing still another embodiment of the present invention. In this embodiment, a sealed enclosure 3o for a diluent is disposed within a sample storage bag 1O. The sealed enclosure 30 of the diluent is compressed by the pressing force of the homogenizer.
It has a structure that can be destroyed. For example, the material of the sealed packaging bag itself may have low mechanical strength, or the seal at the sealed portion may be made to peel easily. The diluent sealed enclosure 30 may be separated from the sample storage bag 10 or may be fixed inside the sample storage bag 10.

FIG. 10 is a schematic cross-sectional view of an embodiment in which a sealed package for a diluent is fixedly provided in the specimen storage bag 10. In this embodiment, the facing sheet 11 constituting the sample storage bag 10 is
．． A sheet 27a of a sheet material having weak adhesion to the sheet material is attached to one of the sheets 12 by heat sealing, for example, and a diluent 28 is sealed therein.

As a result, an emulsion having a desired dilution concentration can be prepared by adding a sample in a desired proportion to the diluent, thereby simplifying the work of the inspector or the inspection device.

FIG. 11a is a plan view of still another embodiment of the present invention, the first
Figure 1 is a schematic sectional view taken along line BB in Figure 11a. In this embodiment, an opening 16 is provided in one of the opposing sheets 11 of the specimen storage bag 10, and a collection container 42 to which a filter 41 is fixed is adhered to the outer surface of the sheet 11 with an adhesive 43 so as to surround the opening 16. After the low-specific gravity particles and high-specific gravity particles in the emulsion are respectively floated and settled, the opening 16 is positioned in the middle between them, the extract is transferred through the filter 41 into the collection container 42, and the container is closed. To separate. The filter 41 needs to have linear pores and a uniform pore cross-sectional area. The size of the pore cross-sectional area may be selected depending on the size of the object to be detected. For example, if the purpose is to test the number of bacteria, the diameter may be set to, for example, about IOμ, taking into consideration the shape and size of the bacteria. If the shape of the pores is complex, there is a risk that bacteria will be captured by the filter and the collected extract will no longer reflect the accurate number of bacteria. An advantage of this embodiment is that the operation of peeling the measuring container 42 from the sample storage bag IO becomes easier. That is, due to the presence of the filter 41, there is no risk of the extract spilling even if the container 42 shakes during peeling. In this embodiment, when collecting the extract, the work of measuring the extract by the inspector or the machine is omitted.

All of the above-described embodiments can be applied to a conventional sample storage bag in which the interior of the sample storage bag IO is divided into two chambers by a mesh sheet.

Although some embodiments of the method of the present invention have been described above, the present invention is not limited to the above-mentioned embodiments, and various modifications can be made without departing from the technical idea of the present invention. It is.

For example, by adjusting the viscosity of the diluent, the flotation and sedimentation rates of low-density particles and high-density particles can be adjusted, and microorganisms in the intermediate layer can be kept in suspension, thereby accurately reflecting the concentration of microorganisms. The extracted liquid can be collected.

Effect of the invention The effects of the present invention are as follows.

(1) To prevent or minimize the incorporation of fat particles and small pieces of crushed material into the extract.

(2) Make it easy to collect the extract of the test substance, whether by manual or mechanized methods, so that anyone can easily do it without the need for skill.

(3) Eliminate or minimize the possibility of the extract coming into contact with workers or machines when collecting the extract.

(4) In the embodiment in which the upper part of the sample storage bag is hermetically sealed, it is easier to dispose of the sample storage bag after extract collection, which reduces the possibility of contaminating machine parts, especially during mechanized processing. eliminate.

[Brief explanation of the drawing]

FIG. 1 is a schematic perspective view of a sample storage bag used in the method of the present invention, FIG. 2a is a schematic perspective view showing a modification of the sample storage bag of FIG. 1, and FIG. A schematic perspective view showing a state in which the specimen storage bag of FIG. 2a is tilted and an elastic pad is positioned in the middle layer when collecting an extract, and FIG. 3 is a specimen storage bag according to another embodiment of the present invention. FIG. 4 is a schematic plan view showing a modification of the embodiment shown in FIG. 3; FIG. 5 is a schematic plan view showing a modification intermediate between the embodiment shown in FIGS. 2 and 3; 6a is a schematic plan view showing a modification of the embodiment of FIG. 5; FIG. 6a is a schematic plan view taken along line B-B of FIG. FIG. 7 is a schematic plan view of a sample storage bag according to another embodiment of the present invention, and FIG. FIG. 9 is a schematic plan view showing still another modification of the embodiment shown in FIGS. 7 and 8. FIG. FIG. 11A is a schematic plan view of a sample storage bag according to still another embodiment of the present invention; FIG. 11A is a schematic enlarged sectional view taken along line B-B in FIG. 11A; It is. Description of symbols IO = Sample storage bag, 11.12: Opposing sheet, 13: Elastic pad, 14: Opening, 15: Sealing seal, 16: Extraction port, 17: Adhesive sheet, 18: Ring-shaped member, 13
a: Notch, 13b: Suction tube insertion guide recess, 19a:
protrusion, 19b: grooved line, 21: sample storage area, 22: extraction area, 23: extraction port, 24: second seal structure, 25:
Extract liquid reservoir, 26 Two extraction liquid chambers, 30: Diluted liquid sealed package, 41: Filter, 42: Collection container, 43: Adhesive,
27: Sheet material, 28: Diluent, FIG, 1 FIG, 6 FIG, 6 Procedural amendment (formality) 1. Indication of the case 2, Name of the invention 3, Name or address of the person making the amendment related to the case 4 , Agent Address: Bunki Yoshida, Patent Application No. 332,427 filed in 1988. Method for collecting extract from specimen and specimen storage bag suitable for said method.

Claims (1)

[Scope of Claims] [1] A predetermined amount of the sample is stored in a sample storage bag, along with a predetermined amount of sterilized diluent if necessary, and a homogenizer is used to repeatedly apply pressing force from outside the bag to fully absorb the sample. In the method of preparing an emulsion of the crushed specimen, and collecting a part of this liquid as an extract, a) storing the specimen in a sterilized specimen storage bag, along with a sterile diluent if necessary; b) Crushing the specimen with a homogenizer to prepare an emulsion; c) Floating the low-density particles in the emulsion and allowing the high-density particles to settle, from the middle part of those layers. A method for collecting an extract from a specimen, characterized by directly collecting the extract. [2] In the method described in claim 1, the method of collecting the extract directly from the intermediate portion includes: a) collecting the crushed specimen with the opening of the specimen storage bag facing upward; A pad made of an elastic material is fixed in advance to the inner or outer surface of one of the facing sheets of the sample storage bag in the middle part of the depth occupied by the emulsion, and b) the low specific gravity particles of the emulsion and A method for collecting an extract from a specimen, characterized in that the extract is collected from the pad area by a syringe-like puncture collection means after floating and settling of high-density particles. [3] In the method according to claim 1, the method for collecting the extract directly from the intermediate portion includes: a) a sample storage bag located close to the opening of the specimen storage bag and facing the specimen storage bag; An elastic pad is fixed in advance to one of the inner or outer surfaces of the sheet, and b) before or after the crushing operation by the homogenizer, the sample storage bag is placed horizontally above the part to which the elastic pad is fixed. b) After the crushing operation, tilt the sample storage bag until the elastic pad is positioned in the middle part of the emulsion, and remove the low specific gravity particles and high specific gravity particles in the emulsion. A method for collecting an extract from a specimen, which is carried out by collecting the extract from the elastic pad region using a puncture collection means after floating and settling. [4] In the method as set forth in claim 1, the method for directly collecting the extract from the intermediate portion includes: a) proximate to the opening of the specimen storage bag and facing the specimen storage bag; A ring-shaped member is fixed in advance to one of the inner or outer surfaces of the sheet, and an extraction port is pre-drilled in the sheet in an area surrounded by the ring-shaped member,
Furthermore, the extraction port is sealed with an adhesive sheet, b) Before or after the crushing operation by the homogenizer, the sample storage bag is hermetically sealed laterally above the part to which the member is fixed, c) ) After the above-mentioned crushing operation, the sample storage bag is tilted until the above-mentioned ring-shaped member is positioned at the above-mentioned middle part of the above-mentioned emulsion, and the floating and settling of low-specific gravity particles and high-specific gravity particles in the above-mentioned emulsion are prevented. A method for collecting an extract from a specimen, characterized in that the method is carried out by peeling off at least a portion of the pressure-sensitive adhesive sheet and draining the extract. [5] In the method according to claim 1, the method for collecting the extract directly from the intermediate portion includes: a) a sample storage bag located close to the opening of the specimen storage bag and facing the specimen storage bag; An elastic pad is fixed in advance to one of the inner or outer surfaces of the sheet, and a cut of a desired width is made in advance in the center of the surface of the elastic pad over the entire thickness of the elastic pad. b) Before or after the homogenizer crushing operation, seal the sample storage bag laterally above the part to which the elastic pad is fixed, and c) After the crushing operation, remove the elastic pad. Tilt the sample storage bag until it is positioned in the middle part of the emulsion, and after the low-specific gravity particles and high-specific gravity particles in the emulsion float and settle, insert the suction tube through the notch in the elastic pad. A method for collecting an extract from a specimen, characterized by: [6] In the method according to claim 1, the method of collecting the extract directly from the intermediate portion includes: a) proximate to the opening of the specimen storage bag and facing the specimen storage bag; An elastic pad is fixed in advance to one of the inner or outer surfaces of the sheet, and a suction tube insertion guide recess is provided in the center of the surface of the elastic pad, and the remaining thickness of the elastic pad is inserted from the bottom of the guide recess. b) Before or after the crushing operation using the homogenizer, place the sample storage bag horizontally above the part to which the elastic pad is fixed. c) after said crushing operation, tilting the specimen storage bag until said elastic pad is positioned in said middle part of said emulsion; and d) separating low specific gravity particles and high density particles in said emulsion. Levitation of specific gravity particles,
A method for collecting an extract from a specimen, characterized in that after sedimentation, the extract is collected by inserting a suction tube through a cut in the elastic pad. [7] The method according to claim 5 or 6, wherein the sheet portion to which the elastic pad is fixed is provided with an opening that communicates with the cut portion of the elastic pad. How to collect. [8] In the method according to claim 5 or 6, an adhesive sheet is attached to the opening surface of the elastic pad or sheet portion, and the adhesive sheet is peeled off when collecting the extract. A method for collecting an extract from a specimen characterized by: [9] In the method according to claim 1, the method of collecting the extract directly from the intermediate portion includes: a) a level occupied by the contents of the specimen storage bag before or after the specimen crushing operation; The sample storage bag is laterally sealed above the level occupied by the contents of the sample storage bag, leaving at least one extraction port for discharging the emulsion above, thereby separating the sample storage area and the extraction area. b) After the above-mentioned crushing operation is completed, the low specific gravity particles and high specific gravity particles of the above emulsion are floated and settled, and c) The above-mentioned sample storage bag is tilted so that the above-mentioned extraction port is separated from the above-mentioned. A method for collecting an extract of a specimen, characterized in that the extract is located in the middle part and is carried out by flowing the extract from the extraction port into the extraction region. [10] The method according to claim 9, wherein the extraction port is formed on both sides of the sample storage bag. [11] In the method according to claim 9 or 10, at least one of both sides of the specimen storage bag is placed above the sealed portion having the extraction port before or after the specimen is crushed. horizontally across the desired width,
A method for collecting an extract of a specimen, comprising forming an extract reservoir in the extraction region. [12] In the method according to claim 9 or 10, before or after the specimen crushing operation, the specimen storage bag is placed over its entire width above the sealed portion having the extraction port. to form an extraction liquid chamber in the extraction region, and after the crushing operation, tilt the specimen storage bag so that the extraction port is positioned in the middle part of the emulsion; Collecting an extract of a specimen, characterized in that the extract is discharged into the extract chamber, and the extract is collected from an intermediate portion between the surface layer and the bottom layer of the extract in the chamber using a puncture collection means. Method. [13] In the method according to claim 12, an elastic pad is fixed in advance to the inner or outer surface of one of the facing sheets of the specimen storage bag in the area where the extraction liquid chamber is to be formed. A method for collecting an extract of a specimen, characterized in that: the extract is collected by the puncture collection means from the elastic pad region. [14] The method according to claim 12, comprising: a) fixing an elastic pad in advance to the inner or outer surface of one of the facing sheets of the specimen storage bag in the region where the extraction liquid chamber is to be formed; b) collecting the extract from the extraction chamber; b) collecting the extract from the extraction chamber; A method for collecting an extract of a specimen, characterized in that the method is performed by inserting a suction tube through a cut in the elastic pad. [15] The method according to claim 12, comprising: a) fixing an elastic pad in advance to the inner or outer surface of one of the facing sheets of the specimen storage bag in the region where the extraction liquid chamber is to be formed; a pipette insertion guide recess is provided in the central portion of the surface of the elastic pad, and a cut of a desired width is made in advance from the bottom of the guide recess to the remaining thickness of the elastic pad; b) A method for collecting an extract of a specimen, characterized in that the extract from the extraction chamber is collected by inserting a suction tube through a cut in the elastic pad. [16] A method for collecting an extract of a specimen according to any one of claims 3 to 16, characterized in that the seal is formed by a heat sealing method. [17] The method according to any one of claims 3 to 16, wherein the seal is fitted with a protrusion formed on the opposing surface of the sample storage bag. A method for collecting an extract of a specimen, characterized in that the extract is formed by a single concave streak. [18] The method according to any one of claims 1 to 17, comprising: a) a sealed package containing a pre-measured amount of sterilized diluent in the sample storage bag; b) breaking the sealed package of the sterilized diluted liquid before or during the crushing operation of the specimen to allow the sterile diluted liquid to flow out; Method. [19] In the method according to claim 1, the method for collecting the extract from the intermediate portion includes providing an extraction port on one side of the facing sheet of the sample storage bag, and providing a filter on the outer surface of the extraction port. After separating the layers of the emulsion, the extraction port is positioned in the middle part, and a predetermined amount of the extract is transferred to the collection container through the filter. A method for collecting an extract from a specimen, comprising: separating a collection container from a specimen storage bag. [20] Store a predetermined amount of the sample in a sample storage bag, along with a predetermined amount of diluent if necessary, and apply pressing force repeatedly from outside the sample storage bag using a homogenizer to sufficiently crush the sample. In a specimen storage bag suitable for a method of preparing an emulsion of a crushed product and collecting a part of the liquid as an extract, a) an elastic material is provided on one of the inner or outer surfaces of the opposing sheets of the specimen storage bag. A specimen storage bag characterized by having a pad fixed to it. [21] Store a predetermined amount of the sample in a sample storage bag, along with a predetermined amount of diluent if necessary, and apply pressing force repeatedly from outside the sample storage bag using a homogenizer to sufficiently crush the sample. In a specimen storage bag suitable for the method of preparing an emulsion of the crushed material and collecting a part of the station as an extract, a) an extraction port is provided on one of the facing sheets of the specimen storage bag, and the extraction A specimen storage bag characterized in that the extraction port is sealed by adhering an adhesive sheet to the outer surface of the mouth. [22] In the specimen storage bag according to claim 21, a ring-shaped member is fixed to one inner or outer surface of the opposing sheet surfaces of the specimen storage bag in a region surrounding the extraction port. A specimen storage bag featuring the following. [23] In the sample storage bag according to any one of claims 21 to 22, the adhesive sheet has an opening on one side and has a predetermined internal volume. A sample storage bag comprising a measuring container portion and a flange portion provided around the measuring container portion, the flange portion being replaced by a measuring container coated with an adhesive. [24] The sample storage bag according to claim 23, characterized in that a filter is provided on the opening surface of the measuring container. [25] Store a predetermined amount of the sample in a sample storage bag, along with a predetermined amount of diluent if necessary, and apply pressing force repeatedly from outside the sample storage bag using a homogenizer to sufficiently crush the sample. In a specimen storage bag suitable for a method of preparing an emulsion of a crushed product and collecting a part of the liquid as an extract, a) an elastic material is provided on one of the inner or outer surfaces of the opposing sheets of the specimen storage bag. A specimen storage bag characterized in that: a pad is fixed; and a notch of a desired width is made in the center of the surface of the elastic pad over the entire thickness of the elastic pad. [26] The sample storage bag according to claim 25, characterized in that a suction tube insertion guide recess is provided on the outer surface of the cut portion of the elastic pad. [27] The specimen storage bag according to claim 25 or 26, characterized in that the sheet portion to which the elastic pad is fixed is provided with an opening that communicates with the cut portion of the elastic pad. bag. [28] The specimen storage bag according to claim 27, characterized in that an adhesive sheet for sealing the notch of the elastic pad or the opening of the sheet portion is attached to the outer surface of the specimen storage bag. Specimen storage bag. [29] Store a predetermined amount of the sample in a sample storage bag, along with a predetermined amount of diluent if necessary, and apply pressing force repeatedly from outside the sample storage bag using a homogenizer to sufficiently crush the sample. In a specimen storage bag suitable for a method of preparing an emulsion of a crushed product and collecting a part of the liquid as an extract, a) when the opening of the specimen storage bag is placed upward, the specimen storage bag A sealing structure is formed on the opposing inner surfaces of the opposing sheets of the sample storage bag, and includes at least one protrusion extending in the lateral direction of the sample storage bag and a corresponding number of grooves in a complementary fitting relationship; A specimen storage bag featuring: [30] The specimen storage bag according to claim 29, characterized in that an elastic pad is fixed to one of the inner or outer surfaces of the facing sheet surface of the specimen storage bag below the seal structure. Specimen storage bag. [31] The sample storage bag according to claim 29, characterized in that at least one extraction port is provided in a portion of the seal structure in the longitudinal direction. [32] The sample storage bag according to claim 29, characterized in that a pair of extraction ports are provided at both ends of the seal structure in the longitudinal direction. [33] In the specimen storage bag according to claim 29, when the opening of the specimen storage bag is arranged upward, the specimen storage bag is stored on the opposing inner surfaces of the opposing sheets of the specimen storage bag. a protrusion and a recess that extend in the lateral direction of the sample storage bag above the liquid level occupied by the expected amount of the contents, and have at least one extraction port and are in a complementary fitting relationship; A first sealing structure is formed by a strip, and a complementary sealing structure is formed further above the first sealing structure and extending laterally over a desired width on at least one of both sides of the sample storage bag. A specimen storage bag characterized in that a second sealing structure is formed by a single protruding line and a single grooved line in a fitting relationship to form an extract reservoir. [34] In the specimen storage bag according to claim 29, when the opening of the specimen storage bag is arranged upward, the specimen storage bag is stored on the opposing inner surfaces of the opposing sheets of the specimen storage bag. a protrusion and a recess that extend in the lateral direction of the sample storage bag above the liquid level occupied by the expected amount of the contents, and have at least one extraction port and are in a complementary fitting relationship; A first sealing structure is formed with a strip, and a strip of protrusion in a complementary fitting relationship extends further above the first sealing structure over the entire width of the sample storage bag in the lateral direction. A specimen storage bag characterized in that a second sealing structure is formed by a strip and a concave strip to form an extraction liquid chamber. [35] In the specimen storage bag according to claim 33, an elastic pad is fixed to one inner or outer surface of the facing sheet surface in the extraction liquid chamber, and the total thickness of the elastic pad is A specimen storage bag characterized in that a cut of a desired width is made in advance in the center of the surface of the elastic pad. [36] The sample storage bag according to claim 34, further comprising: a suction tube insertion guide recess provided on the outer surface of the cut portion of the elastic pad. [37] In the specimen storage bag according to any one of claims 20 to 35, the specimen storage bag is equipped with a sealed package containing a predetermined amount of sterilized diluent; A specimen storage bag featuring: [38] In the specimen storage bag according to any one of claims 20 to 35, the hermetically sealed package for the sterile diluent is located close to the bottom region of the specimen storage bag. forming a pocket formed by heat sealing a sheet material on at least one of the opposing inner surfaces of the specimen storage bag;
After a predetermined amount of diluent is injected into the pocket, the opening of the pocket is heat-sealed so that at least a part of the mechanical strength of the diluent sealed area can be destroyed by the pressing force of the homogenizer. Specimen storage bag. [39] The specimen storage bag according to any one of claims 20 to 37, wherein the inside of the specimen storage bag is made of a mesh material with high mechanical strength and has an opening at the top. A specimen storage bag characterized by being divided into chambers.