JP2015000851A - Body temperature-elevating agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a novel body temperature-elevating agent having a proper body temperature-elevating effect.SOLUTION: The present invention provides a body temperature-elevating agent comprising as the active ingredient the bacterial cell of strain SBC8803 (Deposit No.: FERM BP-10632) belonging to the genus Lactobacillus brevis, or the treated product thereof.

Description

  The present invention relates to a body temperature raising agent.

  In recent years, attention has been paid to an increase in hypothermic children and the elderly. Moreover, even if the body temperature is normal, there are many people who suffer from coldness such as cold end. It is thought that hypothermia and cold cause various discomfort and cause various diseases. Therefore, a body temperature increasing agent capable of increasing body temperature is useful for health. For example, Patent Document 1 describes that lactic acid bacteria Lactobacillus paracasei ST11 strain has an effect of increasing body temperature.

Special table 2011-523407 gazette

  The strain described in Patent Document 1 describes that the body temperature rises by about 1.5 ° C. in a short time of about 60 minutes after ingesting the strain. At such a level of body temperature rise, when a normal heat person ingests the above strain, the body temperature rises to a fever state and is not suitable as a body temperature raising agent.

  Moreover, under stress such as stressful sleep disorder, a decrease in body temperature may be observed due to disorder of lifestyle, excessive cooling, etc., but a strain of lactic acid bacteria that can suppress such a decrease in body temperature has so far been Not known to.

  Then, an object of this invention is to provide the novel body temperature increasing agent which has a moderate body temperature increasing effect.

  The present inventors have found that a strain of Lactobacillus brevis SBC8803, which is a lactic acid bacterium, or a processed product thereof has an effect of appropriately increasing body temperature. The present invention is based on this novel finding.

  That is, this invention provides the body temperature increasing agent which contains the microbial cell of SBC8803 strain which belongs to Lactobacillus brevis, or its processed material as an active ingredient.

  According to the body temperature increasing agent, the body temperature can be appropriately increased. Therefore, for example, it is effective for raising the body temperature of a person having a low body temperature to an appropriate body temperature.

  In addition, the Lactobacillus brevis SBC8803 strain was established on June 28, 2006 at the National Institute of Advanced Industrial Science and Technology, Patent Biological Deposit Center (1st, 1st East, 1st Street, Tsukuba City, Ibaraki Prefecture, Japan, 6th postal code 305-8586). ), And the accession number is FERM BP-10632. In the present specification, this strain is also referred to as “SBL88 strain”.

  Moreover, since the said body temperature increasing agent has the above effects, it can be used also as a cooling improvement agent, for example.

  The present inventors also have the effect that the strain of SBL88 strain or the processed product itself has an action of suppressing a decrease in body temperature under stress such as stressful sleep disorder, lifestyle disturbance, body temperature disorder due to excessive cooling, etc. I also found. Therefore, the said body temperature increasing agent can be used also as a body temperature fall inhibitor.

  Lactobacillus brevis is a type of lactic acid bacterium that has been used for fermented foods for a long time, and has been established to be safe for living organisms. Therefore, the body temperature increasing agent of the present invention is highly safe to the living body and can be taken continuously for a long period of time. Therefore, it can be used as a pharmaceutical ingredient, a food / beverage product component, a food / beverage product additive, and the like.

  ADVANTAGE OF THE INVENTION According to this invention, the novel body temperature raising agent which has a moderate body temperature raising effect is provided. Moreover, the pharmaceutical, food / beverage products, food / beverage additive, etc. containing the said body temperature raising agent are provided.

It is a graph which shows transition of the average body temperature of the mouse | mouth of a SBL88 group and a control group in a non-stress breeding period and a stress breeding period.

  Hereinafter, preferred embodiments of the present invention will be described in detail.

  The body temperature increasing agent of the present invention contains, as an active ingredient, the bacterial body of SBC8803 strain belonging to Lactobacillus brevis or a processed product thereof.

  In the body temperature increasing agent of the present invention, the above bacterial cells and the processed product of the bacterial cells may be used alone or in combination of two or more.

  The microbial cells may be live cells or dead cells. Bacteria can be produced in large quantities by culturing viable bacteria. The medium may be either a liquid medium or a solid medium, but preferably contains a nitrogen source and a carbon source. Nitrogen sources include meat extract, peptone, gluten, casein, yeast extract, amino acids, etc., and carbon sources include glucose, xylose, fructose, inositol, maltose, water candy, soup, starch, bagasse, bran, Molasses, glycerin and the like can be used. Further, ammonium sulfate, potassium phosphate, magnesium chloride, sodium chloride, iron, manganese, molybdenum and the like can be added as inorganic substances, and vitamins and the like can be further added. Suitable media include MRS media, LBS media, Rogosa media, WYP media, GYP media and the like.

  The strain can be cultured according to a culture method commonly used for culturing a strain belonging to Lactobacillus brevis. For example, the culture temperature is usually 20-50 ° C, preferably 25-40 ° C, more preferably 30 ° C. The culture time is usually 6 to 62 hours, preferably 12 to 48 hours, and more preferably 15 to 30 hours. The pH of the medium is usually 3 to 8, preferably 4 to 7, and more preferably 6 to 7. Cultivation may be performed in an incubator, or aeration and shaking may be performed during the cultivation.

  Examples of treated cells include treated products obtained by subjecting the above-mentioned cells (live cells or dead cells) to treatment such as heating, pressurization, drying, crushing, crushing, breaking, or self-dissolution. . Two or more of these treatments may be combined. As the treated product of the microbial cells, for example, a treated product obtained by heating the microbial cells at 100 ° C. or more for several minutes or more (for example, by subjecting the microbial cells to autoclaving at a temperature of 105 to 125 ° C. for 10 minutes or more Processed product), processed product obtained by freeze-drying, spray-drying, etc. on the bacterial cells, processed product obtained by contacting the bacterial cells with an organic solvent (acetone, ethanol, etc.), A treated product obtained by contacting with an alkaline solution, a treated product obtained by enzymatic disruption of bacterial cells, or a treated product obtained by physically destroying the bacterial cells with ultrasonic waves, a French press, or the like can be used. Such a cell-treated product is preferable in that it is easy to handle as compared with untreated cells (particularly live cells).

  The body temperature increasing agent of the present invention may be in any form such as solid (for example, powder obtained by freeze-drying), liquid (water-soluble or fat-soluble solution or suspension), paste, etc. Any dosage form such as powder, pill, granule, tablet, syrup, troche, capsule and the like may be used.

  The above-mentioned various preparations may consist only of the above-mentioned fungus bodies or processed products thereof, which are active ingredients, and can be prepared, for example, by molding the fungus bodies or treated products thereof into the above dosage form. . The above-mentioned various preparations also contain the above active ingredients and pharmaceutically acceptable additives (excipients, binders, lubricants, disintegrants, emulsifiers, surfactants, bases, solubilizers, suspensions). It is also possible to prepare the mixture by mixing with an agent and the like. In this case, the content of the active ingredient is, for example, 0.5 to 50% by mass based on the total amount of the preparation.

  For example, the excipient includes lactose, sucrose, starch, dextrin and the like. Examples of the binder include polyvinyl alcohol, gum arabic, tragacanth, gelatin, hydroxypropylmethylcellulose, hydroxypropylcellulose, sodium carboxymethylcellulose, polyvinylpyrrolidone and the like. Examples of the lubricant include magnesium stearate, calcium stearate, talc and the like. Examples of the disintegrant include crystalline cellulose, agar, gelatin, calcium carbonate, sodium bicarbonate, dextrin and the like. Examples of the emulsifier or surfactant include Tween 60, Tween 80, Span 80, and glyceryl monostearate. Examples of the base include cetostearyl alcohol, lanolin, polyethylene glycol, rice bran oil, fish oil (DHA, EPA, etc.), olive oil and the like. Examples of the solubilizer include polyethylene glycol, propylene glycol, sodium carbonate, sodium citrate, Tween 80 and the like. Examples of the suspending agent include Tween 60, Tween 80, Span 80, glyceryl monostearate, polyvinyl alcohol, polyvinyl pyrrolidone, methyl cellulose, hydroxymethyl cellulose, sodium alginate and the like.

  The body temperature increasing agent of the present invention may be administered to humans or non-human mammals. The dosage and administration method can be appropriately determined according to the condition, age, etc. of the individual to be administered. Suitable administration methods include, for example, oral administration. As an example of the dosage and administration method, a method of orally administering a body temperature increasing agent in an amount of an active ingredient amount of 0.5 mg to 500 mg once a day can be mentioned.

  The body temperature increasing agent of the present invention can be used as a pharmaceutical ingredient, a food / beverage product component, a food / beverage product additive, a feed component, a feed additive, and the like.

  For example, the body temperature increasing agent of the present invention is water, soft drinks, fruit juice drinks, milk drinks, alcoholic drinks, breads, noodles, rice, tofu, dairy products, fermented foods, fermented milk, soy sauce, miso, confectionery. It can be used as an additive to foods and beverages. These foods and drinks may further contain other additives usually used in the art, and examples of such additives include bitters, flavors, apple fibers, soybean fibers, meat extracts, blacks. Vinegar extract, gelatin, corn starch, honey, animal and vegetable oils; monosaccharides such as glucose and fructose; disaccharides such as sucrose; polysaccharides such as dextrose and starch; sugar alcohols such as erythritol, xylitol, sorbitol, and mannitol; vitamin C and the like Vitamins. The body temperature increasing agent of the present invention can also be used as a component for foods for specified health use, foods for special uses, dietary supplements, health foods, functional foods, foods for the sick, and the like. The said food-drinks can be obtained with the manufacturing method including the process of adding the body temperature increasing agent of this invention. The food and drink containing the body temperature increasing agent of the present invention may be a fermented product obtained by fermenting milk, skim milk, soy milk, vegetables, fruit juice, grains, processed products thereof, and the like with the above strain.

  The body temperature increasing agent of the present invention has an effect of appropriately increasing body temperature. That is, according to the body temperature increasing agent of the present invention, for example, it can be increased by 0.05 to 0.50 ° C. Therefore, even if ingested by a person with normal heat, the body temperature does not rise to a fever state, and thus can be used as a cold improving agent for improving symptoms such as cold.

  Moreover, the body temperature increasing agent of this invention has the effect of suppressing the body temperature fall at the time of body temperature disorder | damage | failure by lifestyle disorder, excessive cooling, etc. under stress, such as stress sleep disorder. Although body temperature tends to be lower than that at normal time in these body temperature disorders, by taking the body temperature increasing agent of the present invention, it is possible to suppress a decrease in body temperature and maintain a body temperature closer to normal. Therefore, the body temperature increasing agent of the present invention can be used as a body temperature lowering inhibitor.

  Hereinafter, the present invention will be described more specifically based on examples and the like. However, the present invention is not limited to the following examples.

[Example 1]
(Test method)
<Preparation of treated cells>
SBL88 strain was inoculated into a medium (composition: maltose 2 mass%, yeast extract 1.4 mass%, sodium acetate 0.5 mass%, manganese sulfate 0.005 mass%, pH 6.5-7.0), 30 The culture was allowed to stand at 1 ° C. for 1 day. The obtained culture solution (about 8 × 10 ⁸ cfu / ml) was centrifuged at 8,000 rpm for 10 minutes, and the cells were collected. The collected cells were resuspended in distilled water and centrifuged at 8,000 rpm for 10 minutes to collect the cells. This operation was repeated twice. The collected cells were suspended in distilled water, heat-treated at 105 ° C. for 10 minutes, and then freeze-dried to obtain heat-treated cell powder (cell-treated product).

<Preparation of mouse feed>
After adding 0.5% by mass of SBL88 strain treated cells to powdered feed AIN-93M (produced by Oriental Yeast Co., Ltd.), pelleted mouse feed containing the treated cells (SBL88-containing AIN-93M feed) Was prepared. As a control, a powdered feed AIN-93M was pelleted to prepare a mouse feed (AIN93M feed) that does not contain a treated cell product.

<Mouse breeding>
Throughout the period, mice were raised in a rotating cage (SW-15S, Merquest Co.). The amount of activity of mice was measured using a chronobiology kit (Stanford Software Systems, CA).

  C3H / HeN strain mice (6 weeks old male, 13 mice, SLC Japan) were bred for 10 days. Three days after the start of habituation breeding, a temperature data logger (manufactured by Thermocron SL KN Laboratory) was embedded in the abdominal cavity of the mouse. After acclimatization, mice were divided into 2 groups in order of total activity, AIN-93M diet was used as diet for 6 animals in the control group, and AIN-93M containing SBL88 as food for 7 animals in the test group (SBL88 group). Feeded and allowed to eat freely.

<Measurement of average body temperature>
One day after the start of feeding the AIN-93M diet or the SBL88-containing AIN-93M diet, body temperature measurement was started using a data logger. The measurement was performed every 15 minutes, and the average body temperature for one day (24 hours) was obtained. The average body temperature of each group was determined as the average body temperature of the mice in each group. The presence or absence of significant difference was compared between groups by the Tukey method after multiple comparison test.

<Stress load on mice>
In the SBL88 group and the control group, the period from the measurement start date (day 0) to the 26th day was set as the non-stress breeding period, and the stress breeding period was started from the 27th day. As a stress loading method, stress sleep disorder was continuously induced for 2 weeks by physically blocking and restricting the mouse from getting off the rotating wheel.

<Result>
FIG. 1 is a graph showing changes in average body temperature of mice in the SBL88 group and the control group during the non-stress breeding period and the stress breeding period. In FIG. 1, the data marked with * indicates p <0.05, and the data marked with ** indicates p <0.01. In the non-stress breeding period (0-26 days), the SBL88 group showed a statistically significant difference in average body temperature compared to the control group.

  Even during the stress breeding period (27th to 41st days), the SBL88 group showed a statistically significant higher value of the average body temperature than the control group. Further, in the stress breeding period, the difference in average body temperature between the SBL88 group and the control group was more remarkable than in the non-stress breeding period. In the control group, it was observed that when stress was applied, the average body temperature gradually decreased greatly compared to the non-stress breeding period. On the other hand, in the SBL88 group, even when stress was applied, a decrease in body temperature was suppressed and a body temperature closer to normal was maintained. The difference in average body temperature between the SBL88 group and the control group was 1 ° C. or less under both unstressed and stressed conditions. That is, the body temperature was able to be raised moderately by administration of the cell-treated product of SBL88. Moreover, in the stress load state, the fall of body temperature was able to be suppressed by administration of the microbial cell processed material of SBL88.

Claims (6)

  A body temperature increasing agent comprising a cell of SBC8803 (accession number: FERM BP-10632) belonging to Lactobacillus brevis or a processed product thereof as an active ingredient.   The body temperature increasing agent according to claim 1, which is a cooling improver.   The body temperature increasing agent according to claim 1, which is a body temperature lowering inhibitor.   A pharmaceutical comprising the body temperature increasing agent according to claim 1.   Food / beverage products containing the body temperature increasing agent of Claim 1.   The food-drinks additive containing the body temperature raising agent of Claim 1.

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