JP2014532406A - 組換えタンパク質発現のための操作された下等真核宿主株 - Google Patents

組換えタンパク質発現のための操作された下等真核宿主株 Download PDF

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Publication number
JP2014532406A
JP2014532406A JP2014538873A JP2014538873A JP2014532406A JP 2014532406 A JP2014532406 A JP 2014532406A JP 2014538873 A JP2014538873 A JP 2014538873A JP 2014538873 A JP2014538873 A JP 2014538873A JP 2014532406 A JP2014532406 A JP 2014532406A
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Japan
Prior art keywords
host cell
att1
polypeptide
seq
activity
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Pending
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JP2014538873A
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English (en)
Japanese (ja)
Inventor
ジアーン，ボー
アーギロス，レベツカ，デイー
ネルソン，ステフアニー
デイビツドソン，ロバート，シー
チエン，ローンフア
ジユアーン，ジユイン
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Merck Sharp and Dohme LLC
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Merck Sharp and Dohme LLC
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Application filed by Merck Sharp and Dohme LLC filed Critical Merck Sharp and Dohme LLC
Publication of JP2014532406A publication Critical patent/JP2014532406A/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Oncology (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2014538873A 2011-10-28 2012-10-23 組換えタンパク質発現のための操作された下等真核宿主株 Pending JP2014532406A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161552674P 2011-10-28 2011-10-28
US61/552,674 2011-10-28
PCT/US2012/061432 WO2013062940A1 (en) 2011-10-28 2012-10-23 Engineered lower eukaryotic host strains for recombinant protein expression

Publications (1)

Publication Number Publication Date
JP2014532406A true JP2014532406A (ja) 2014-12-08

Family

ID=48168381

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2014538873A Pending JP2014532406A (ja) 2011-10-28 2012-10-23 組換えタンパク質発現のための操作された下等真核宿主株

Country Status (9)

Country Link
US (1) US9328367B2 (OSRAM)
EP (1) EP2771461B1 (OSRAM)
JP (1) JP2014532406A (OSRAM)
KR (1) KR20140091018A (OSRAM)
CN (1) CN104024409A (OSRAM)
AU (1) AU2012329091A1 (OSRAM)
CA (1) CA2853341A1 (OSRAM)
IN (1) IN2014CN02963A (OSRAM)
WO (1) WO2013062940A1 (OSRAM)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018038286A (ja) * 2016-09-05 2018-03-15 国立大学法人神戸大学 新規宿主細胞及びそれを用いた目的タンパク質の製造方法

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3023438B1 (en) 2009-09-03 2020-03-11 Merck Sharp & Dohme Corp. Anti-gitr antibodies
AU2012329091A1 (en) 2011-10-28 2014-05-01 Merck Sharp & Dohme Corp. Engineered lower eukaryotic host strains for recombinant protein expression
WO2013123034A1 (en) * 2012-02-16 2013-08-22 Merck Sharp & Dohme Corp. Methods for reducing mannosyltransferase activity in yeast
WO2014066134A1 (en) * 2012-10-22 2014-05-01 Merck Sharp & Dohme Corp. Crz1 mutant fungal cells
US11203620B2 (en) * 2015-03-03 2021-12-21 Novartis Ag Constitutive yeast LLP promotor-based expression systems
WO2017194699A1 (en) * 2016-05-13 2017-11-16 University Of Copenhagen A cell-based array platform

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060148000A1 (en) * 1999-04-21 2006-07-06 Wyeth Methods for identifying modulators of N-type ion channel inactivation
JP2002541863A (ja) * 1999-04-21 2002-12-10 ワイス Ｎ−型イオンチャンネル不活性化のモジュレーターを同定する方法
WO2001081548A1 (en) * 2000-04-24 2001-11-01 Wyeth Novel cell systems having specific interaction of peptide binding pairs
DE60139467D1 (de) 2000-05-17 2009-09-17 Mitsubishi Tanabe Pharma Corp Verfahren zur herstellung von proteinen unter reduktion von mannosephosphat in der zuckerkette und dadurch hergestelltes glykoprotein
AU7684201A (en) 2000-06-28 2002-01-08 Glycofi Inc Methods for producing modified glycoproteins
US7795002B2 (en) 2000-06-28 2010-09-14 Glycofi, Inc. Production of galactosylated glycoproteins in lower eukaryotes
US7259007B2 (en) 2003-12-24 2007-08-21 Glycofi, Inc. Methods for eliminating mannosylphosphorylation of glycans in the production of glycoproteins
CA2565125A1 (en) * 2004-04-29 2005-11-10 Glycofi, Inc. Methods for reducing or eliminating alpha-mannosidase resistant glycans in the production of glycoproteins
CN101606769A (zh) 2009-07-15 2009-12-23 陈德芳 一种宝石连接结构
AR087433A1 (es) * 2011-08-08 2014-03-26 Merck Sharp & Dohme Analogos de insulina n-glicosilados
AU2012329091A1 (en) 2011-10-28 2014-05-01 Merck Sharp & Dohme Corp. Engineered lower eukaryotic host strains for recombinant protein expression

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE GENBANK [ONLINE], ACCESSIN NO. FR839628, <HTTP://WWW.NCBI.NLM.NIH.GOV/NUCCORE/328349772?SAT, JPN6016031931 *
NUCLEIC ACIDS RES. (1993) VOL.21, NO.1, P.69-77, JPN6016031928 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018038286A (ja) * 2016-09-05 2018-03-15 国立大学法人神戸大学 新規宿主細胞及びそれを用いた目的タンパク質の製造方法

Also Published As

Publication number Publication date
AU2012329091A1 (en) 2014-05-01
US9328367B2 (en) 2016-05-03
US20140302557A1 (en) 2014-10-09
EP2771461A4 (en) 2015-06-17
IN2014CN02963A (OSRAM) 2015-07-03
CN104024409A (zh) 2014-09-03
CA2853341A1 (en) 2013-05-02
WO2013062940A1 (en) 2013-05-02
KR20140091018A (ko) 2014-07-18
EP2771461A1 (en) 2014-09-03
EP2771461B1 (en) 2019-12-04

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