JP2014223038A5 - - Google Patents

Claims (11)

  A test gene is introduced into a cell into which a gene encoding a terpene synthase variant that encodes a terpene synthase variant that has an enzyme activity and / or enhanced expression compared to a wild-type enzyme. A screening method for a terpene synthase gene and / or a mutant thereof, comprising selecting a test gene introduced into a living cell among test genes introduced, cultured, and introduced into the cell.   The method for screening a terpene synthase gene and / or a mutant thereof according to claim 1, wherein the terpene synthase mutant is a terpene synthase mutant that causes cell death in cells expressing the mutant.   The terpene synthase according to claim 2, wherein the cell death is cell death caused by a substrate consumed by the terpene synthase mutant or a compound serving as a material of the substrate being a compound involved in cell survival. A screening method for genes and / or mutants thereof.   The method for screening a terpene synthase gene and / or a variant thereof according to any one of claims 1 to 3, wherein the terpene synthase mutant is a geraniol synthase mutant.   The method for screening a terpene synthase gene and / or variant thereof according to any one of claims 1 to 4, wherein the cell is Escherichia coli.   A gene encoding a geraniol synthase mutant with enhanced enzyme activity, wherein the mutant has enhanced enzyme activity and / or expression compared to a wild-type enzyme and expresses the mutant In a cultured cell, a cell into which a gene encoding a mutant that causes cell death caused by a substrate consumed by the mutant or a compound serving as a material of the substrate being a compound involved in cell survival is introduced into a cell. A screening method for a terpene synthase gene and / or a variant thereof, comprising introducing a test gene and culturing, and selecting a test gene introduced into a living cell among test genes introduced into the cell.   A gene encoding a geraniol synthase mutant with enhanced enzyme activity, wherein the mutant has enhanced enzyme activity and / or expression compared to a wild-type enzyme and expresses the mutant In E. coli, the substrate that is consumed by the mutant or the compound that is the material of the substrate is a compound that is involved in the survival of E. coli. A screening method for a terpene synthase gene and / or a mutant thereof, which comprises selecting a test gene introduced into living E. coli among test genes introduced into E. coli after culturing by introducing the test gene. The terpene synthase according to claim 6 or 7 , wherein the gene encoding the geraniol synthase mutant with enhanced enzyme activity is a gene represented by the nucleotide sequence set forth in SEQ ID NO: 1 or SEQ ID NO: 3. Screening method for genes and / or mutants thereof.   Of the test genes introduced into the cells, the test genes are introduced into the cells into which the gene encoding the geraniol synthase mutant represented by the nucleotide sequence shown in SEQ ID NO: 1 or SEQ ID NO: 3 has been introduced. A method for screening a terpene synthase gene and / or a mutant thereof, comprising selecting a test gene introduced into a living cell.   Among the test genes introduced into E. coli, the test gene was introduced and cultured in E. coli into which the gene encoding the geraniol synthase mutant represented by the nucleotide sequence shown in SEQ ID NO: 1 or SEQ ID NO: 3 was introduced. A screening method for a terpene synthase gene and / or a mutant thereof, comprising selecting a test gene introduced into living E. coli.   A test gene is introduced into E. coli transformed with the plasmid represented by the nucleotide sequence shown in SEQ ID NO: 5 or SEQ ID NO: 6 and cultured, and the test gene introduced into the surviving cells among the test genes introduced into the cells. A method for screening a terpene synthase gene and / or a mutant thereof, comprising selecting a test gene.