JP2014211308A - Liquid drop auxiliary device - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a liquid drop auxiliary device capable of preventing scattering of the liquid caused by foaming or the like.SOLUTION: A resin substrate 50 functioning as a liquid drop auxiliary device has a film 52 pasted on a surface (lower surface) having a contact with a circular disk for collecting blood and on a surface (upper surface) on the opposite side, and when a discharge part (nozzle) breaks the film 52 and sends the blood up to a hole inlet (flow path entrance of the circular disk) through an open hole 51 so as to be dropped, the blood which is foamed and cannot be dropped into the hole inlet (flow path entrance) enters again the hole inlet without leaking to the outside through the film 52. As a result, scattering of the blood caused by foaming or the like can be prevented.

Description

  The present invention relates to a liquid dropping auxiliary device used for a container having a hole for dropping and collecting a liquid.

  The liquid dropping auxiliary device is used for a liquid collecting device, for example. As an example of the liquid collection device, a blood collection device for collecting blood, that is, collecting blood will be described. Blood collection devices are used for quantitative analysis in nuclear medicine diagnosis (for example, PET (Positron Emission Tomography), SPECT (Single Photon Emission CT), etc.), and in particular, the concentration of radioactivity in arterial blood of small animals (eg, mice, rats, etc.). Used for measurement.

  Specifically, blood is collected (collected) after administration of a radiopharmaceutical to a small animal, and plasma separation is performed by centrifugation after completion of the whole blood collection at a predetermined time. Changes in radioactivity concentration in whole blood and plasma over time Is measured (for example, see Patent Documents 1 and 2). As shown in FIG. 5, blood is continuously fed from a catheter 14 into a microfluidic device (liquid dividing device) 40 made of PDMS resin (Polydimethylsiloxane). When a predetermined blood collection amount is reached by a CCD camera on the microfluidic device 40, a valve (not shown) is operated to control the flow, and gas is supplied from the bubble pipe 46 to the main flow path 13 through the side path 42. The blood to be measured is sent to the disc (CD well) 24. As shown in FIG. 2, the disk 24 is formed with 36 U-shaped flow passages 26 radially. It passes through the nozzle 23 (refer to FIG. 5) sent out by the gas and drops at the inlet (flow channel inlet 25) of the U-shaped flow channel 26 and enters the flow channel 26.

  In the above-described method, when a very small amount of liquid such as blood having a surface-active action is sent out by air of a constant pressure, it does not form a droplet but foams at the tip of the discharge portion represented by the nozzle 23. As a result, there arises a problem that foamed blood overflows from the inlet of the U-shaped channel 26 and does not enter the entire amount, and further, blood that overflows from the channel inlet 25 mixes into the adjacent channel.

  Therefore, a liquid dropping auxiliary device used for a container represented by a disk 24 is used in order to cause a liquid bubble to be collected represented by blood or the like to drop into a flow path without being scattered outside the flow path inlet 25. Yes (see, for example, Patent Document 3). As shown in FIG. 6, the liquid dropping auxiliary device is formed from a resin substrate 150 having an injection port 150 a that is in contact with the disk 24 and that communicates with the inlet of the U-shaped channel 26 of the disk 24. Become. Further, the resin substrate 150 has an air hole 150b, and air can be released through the air hole 150b.

International Publication No. WO2009-093306 JP 2011-0754200 A Japanese Patent No. 3955618

  However, in the case of a resin substrate having such air holes, there is a problem that the resin substrate must be processed by a photolithography technique or the like. Therefore, it is desired to prevent scattering of liquid due to foaming or the like using a conventional through hole (injection port) without performing special processing such as air holes.

  This invention is made in view of such a situation, and it aims at providing the liquid dripping auxiliary | assistance apparatus which can prevent scattering of the liquid by foaming.

In order to achieve such an object, the present invention has the following configuration.
That is, the liquid dropping auxiliary device according to the present invention (the former invention) is a liquid dropping auxiliary device used for a container having a hole for dropping and collecting a liquid. It has a through-hole communicating with the hole, and is provided with a thin film attached to the surface opposite to the surface in contact with the container.

  [Operation / Effect] According to the liquid dropping auxiliary device according to the present invention (the former invention), there is a hole for dropping and collecting the liquid before discharging the liquid from the tip of the discharge portion toward the liquid dropping auxiliary device. The container and the liquid dropping auxiliary device are set so that the hole coincides with the through hole of the liquid dropping auxiliary device. The liquid dripping auxiliary device is provided with a thin film attached to the surface opposite to the surface in contact with the container so that the liquid does not leak out of the liquid dripping auxiliary device. Liquid is discharged when the discharge part breaks the thin film and the tip of the discharge part enters the inside of the through hole, and the liquid is dropped to the entrance of the hole through the through hole. The liquid that foamed during the dropping of the liquid and could not be dropped at the entrance of the hole again enters the entrance of the hole without leaking out due to the thin film. As a result, scattering of liquid due to foaming or the like can be prevented.

  Moreover, the liquid dropping auxiliary device according to the present invention (the latter invention) is a liquid dropping auxiliary device used for a container having a hole for dropping and collecting a liquid. It has a through hole communicating with the hole, and the diameter of the through hole on the surface side in contact with the container is larger than the diameter of the through hole on the surface side opposite to the surface in contact with the container. is there.

  [Operation / Effect] According to the liquid dropping auxiliary device according to the present invention (the latter invention), there is a hole for dropping and collecting the liquid before discharging the liquid from the tip of the discharge portion toward the liquid dropping auxiliary device. The container and the liquid dropping auxiliary device are set so that the hole coincides with the through hole of the liquid dropping auxiliary device. In the liquid dripping auxiliary device, the diameter of the through hole on the surface side in contact with the container is larger than the diameter of the through hole on the surface side opposite to the surface in contact with the container. A structure in which the through-hole is not used as a sealed space is possible only by reducing the diameter of the through-hole on the surface (the non-contact surface). Therefore, when the liquid is discharged when the tip of the discharge portion enters the inside of the through hole and the liquid is dropped to the entrance of the hole through the through hole, the escape path of the gas (for example, air) sent together with the liquid Can be secured. Therefore, the liquid enters the hole entrance without being blown by the gas. As a result, scattering of liquid due to foaming or the like can be prevented.

  In the latter invention described above, the diameter of the through hole on the contact surface side is equal to or smaller than the diameter of the hole of the container, and the diameter of the through hole on the surface side opposite to the contact surface is discharged toward the liquid dropping auxiliary device. More preferably, it is larger than the diameter of the tip of the discharge part. When the liquid is dropped from the tip of the discharge part to the inlet of the hole through the through hole, the diameter gradually increases toward the downstream side, so that the liquid does not flow backward or scatter to the upstream side. A liquid can be reliably dripped toward.

  In the example of the latter invention described above, the inner wall surface of the through hole is formed in a slope shape so that the diameter of the through hole on the surface side in contact with the (container) is smaller than that on the surface side opposite to the surface in contact with It is to form larger than the diameter. Another example of the latter invention described above is that the inner wall surface of the through-hole is formed in a stepped shape so that the diameter of the through-hole on the surface in contact with the (container) is the surface opposite to the surface in contact with It is to form larger than the diameter of the through hole.

  In these inventions described above (including the former invention and the latter invention), an example of the liquid is blood. Since blood is highly viscous and has a surface-active effect, it is possible to prevent blood scattering due to foaming or the like when applied to blood in these inventions.

According to the liquid dripping auxiliary device according to the present invention (the former invention), the liquid dripping auxiliary device is provided with a thin film pasted on the surface opposite to the surface in contact with the container, and the discharge part breaks through the thin film and penetrates. When the liquid is dropped to the entrance of the hole through the hole, the liquid which has foamed and could not be dropped to the entrance of the hole again enters the entrance of the hole without leaking out due to the thin film. As a result, scattering of liquid due to foaming or the like can be prevented.
Further, according to the liquid dropping auxiliary device according to the present invention (the latter invention), the diameter of the through hole on the surface side in contact with the container is larger than the diameter of the through hole on the surface side opposite to the surface in contact with the container. Therefore, since the inside of the through hole is not a sealed space, a gas escape path can be secured, and the liquid enters the hole entrance without being blown by the gas. As a result, scattering of liquid due to foaming or the like can be prevented.

It is a schematic perspective view of the blood collection apparatus and measurement apparatus which concern on each Example. It is a schematic plan view of the disc which concerns on each Example. (A) is a schematic plan view of the resin substrate which concerns on Example 1, (b) is AA arrow sectional drawing of (a). (A) is a schematic top view of the resin substrate which concerns on Example 2, (b) is AA arrow sectional drawing of (a), (c) is (a) of embodiment different from (b). It is AA arrow sectional drawing. It is a schematic perspective view of the conventional blood collection apparatus and measurement apparatus. It is a schematic perspective view of the resin substrate with the conventional air hole.

Embodiment 1 of the present invention will be described below with reference to the drawings.
FIG. 1 is a schematic perspective view of a blood collection device and a measurement device according to each embodiment. In Example 1, including Example 2 described later, blood is taken as an example of the liquid to be collected, and a resin substrate is taken as an example of a liquid dropping auxiliary device used in the blood collecting device. explain. Moreover, about the film used as a thin film in the present Example 1, illustration is abbreviate | omitted in FIG. The components common to those in FIG. 5 are denoted by the same reference numerals.

  As shown in FIG. 1, the blood collection apparatus 10 according to the first embodiment collects blood to be measured by separating it in time series. In addition, a measuring device 30 that measures radiation (for example, β rays, γ rays, etc.) contained in blood collected by the blood collecting device 10 is provided around the blood collecting device 10. In Example 1, including Example 2 to be described later, blood after administration of the radiopharmaceutical into the body of the mouse is collected (that is, blood is collected), and the radiation contained in the blood is measured. In addition, plasma separation by centrifugation is performed, and radiation contained in the plasma and blood cells separated from the plasma is measured.

  The blood collection apparatus 10 includes a microfluidic device (liquid dividing device) 40 configured by vertically stacking two PDMS substrates 11 and 12 made of PDMS resin (Polydimethylsiloxane). The PDMS substrates 11 and 12 are grooved with a predetermined dimension, and the main flow path 13 and the side paths 41, 42, and 43 are formed by the grooves. Here, the material of the blood collection device 10 is not limited to PDMS, and may be any material that is optically transparent such as acrylic, polycarbonate, COP (cycloolefin polymer).

  A catheter 14 is disposed on the blood inlet side of the main channel 13, and the main channel 13 and the catheter 14 are connected via a connector 15. Blood is continuously fed from the catheter 14 into the main channel 13 and the amount of inflow is controlled by a valve (not shown). A blood pipe 16 is disposed on the blood outlet side of the main flow path 13, and the main flow path 13 and the blood pipe 16 are connected via a connector 17.

  A light source 21 and a photodiode 22 are disposed across the main flow path 13. The blood flowing in the main flow path 13 or heparin solution described later is irradiated with light from the light source 21, and the photodiode 22 detects the light shielding by the blood, so that the blood or heparin solution is optically monitored (monitored) as described later. Measure length information of blood or heparin solution. Here, the light source 21 and the photodiode 22 have been described as an example of the optical measurement means. However, any means for measuring the liquid interval while optically monitoring the liquid to be measured can be used as the light source 21 and the photodiode 22. It is not limited. For example, volume information of the liquid to be measured may be acquired by a CCD camera. In addition, the light source 21 and the photodiode 22 are so-called “transmission type sensors” that are arranged to face each other with the main flow channel 13 interposed therebetween as shown in FIG. On the other hand, a so-called “reflective sensor” may be used in which light detection means typified by a photodiode is provided on the same side, and detection is performed using reflected light from blood.

  On the other hand, a nozzle 23 is connected to the downstream side of the blood pipe 16 described above. As the nozzle 23, a capillary tube such as an injection needle or a glass tube is used. Here, although the nozzle 23 is used as the discharge unit for discharging the liquid, a dispenser may be used. A disk (also called “CD well”) 24 that receives and stores blood dropped from the nozzle 23 is provided. On the center side of the disk 24, flow path inlets 25 (see FIG. 2) each having a plurality of openings for receiving the dropped blood are arranged radially. The circular plate 24 is also grooved in the same manner as the PDMS substrates 11 and 12 described above, and a plurality of U-shaped flow paths 26 each including a U-shaped groove are formed by the grooves (FIG. 2). Are formed radially. Each U-shaped channel 26 is connected to the outer end of the above-described channel inlet 25 on a one-to-one basis, and each U-shaped channel 26 is formed to extend in the radial direction of the disk 24. . Thus, by interposing the nozzle 23, the disc 24 is formed so that blood can flow through the main flow path 13. The disc 24 corresponds to the container in the present invention, and the flow path inlet 25 and the U-shaped flow path 26 correspond to the holes of the container in the present invention. A specific configuration of the disc 24 will be described later with reference to FIG.

On the other hand, the measuring device 30 includes a reading unit 31. The reading unit 31 is provided with a cover for inserting the exposed imaging plate IP, and detects β ⁺ rays contained in the blood by reading the light excited from the imaging plate IP. . Specifically, as shown in FIG. 1B, the reading unit 31 includes a laser light source 32 and a photomultiplier tube (photomultiplier tube) 33, and a laser is applied from the laser light source 32 to the imaging plate IP. , And the photomultiplier tube 33 converts the light excited by the laser irradiation of the imaging plate IP into electrons and multiplies them, thereby detecting β ⁺ rays simultaneously two-dimensionally.

  As described above, the microfluidic device 40 includes the main flow path 13 for feeding blood, the side path 41 for feeding a heparin solution which is a kind of anticoagulant for preventing the occurrence of blood coagulation, and the side path for feeding air or gas. 42 and a side passage 43 for discharging blood or heparin solution.

  A cleaning liquid pipe 44 is disposed on the solution inlet side of the side path 41, and the side path 41 and the cleaning liquid pipe 44 are connected via a connector 45. If necessary, the heparin solution is poured into the main flow path 13 from the cleaning liquid pipe 44 via the side path 41 to clean the flow path. The inflow of heparin solution is controlled by a valve. Anticoagulants are not limited to heparin solutions.

  A bubble piping 46 is disposed on the gas inlet side of the side passage 42, and the side passage 42 and the bubble piping 46 are connected via a connector 47. The inflow time of air or gas controlled by a pressure generator (not shown) is adjusted by a valve and sent to the main flow path 13 through the side path 42. The bubbles are used to extract blood based on blood length information and to discharge waste liquid (blood, heparin solution, or a mixture thereof) remaining in the flow path of the microfluidic device 40. Here, the gas to be fed is not limited, and may be any gas that does not react with blood or heparin solution, as exemplified by rare gas such as helium, neon, and argon, or nitrogen gas.

  The bubble pipe 46 sends gas (for example, air or gas) through the side passage 14 to the main flow path 13 and inserts the gas as bubbles at a specified predetermined interval, whereby the blood to be measured is timed. Separated in series and sent to the disc 24. That is, the bubbles serve as a separator. In addition, although gas was used as a separator, it is not limited to gas, If there is little possibility of mixing with the liquid of measurement object (in each Example 1, 2) or there is no possibility, it is measurement object A liquid other than this liquid may be used as the separator. When the liquid to be measured is blood as in Example 1, including Example 2 described later, a liquid that does not mix with blood, such as mineral oil or fluorine oil, is used. It may be used as a separator. However, when a liquid is used as a separator, it can be used as a separator because it comes into contact with blood, but it is not desirable in that it is sent to the disk 24 and collected.

  A waste liquid pipe 48 is provided on the side of the waste liquid outlet side of the side path 43, and the side path 43 and the waste liquid pipe 48 are connected via a connector 49. The discharge amount is adjusted by a valve, and blood other than blood to be collected, heparin solution after channel cleaning, or a mixed solution thereof is discharged as waste liquid.

  Further, a valve is disposed downstream of the connector 15 of the main flow path 13, and a valve is disposed upstream of the connector 17, the light source 21, and the photodiode 22 of the main flow path 13. A valve is disposed downstream of the connector 45 of the side passage 41, and a valve is disposed downstream of the connector 47 of the side passage 42. A valve is disposed upstream of the connector 49 in the side passage 43.

  Next, a specific configuration of the disc 24 will be described with reference to FIG. 2 including FIG. FIG. 2 is a schematic plan view of a disk according to each embodiment.

  As shown in FIG. 2, the U-shaped flow path 26 of the disk 24 is formed by connecting the flow path inlet 25 and the air hole 27 described above. When the flow channel inlet 26 that is a blood inlet is the upstream portion of the blood and the air hole 27 is the downstream portion, the U-shaped flow channel 26 extends inward in the radial direction of the disk 24 from the upstream portion to the downstream portion. The U-shape is formed by extending from the outside toward the outside, turning back and extending from the outside toward the inside in the radial direction of the disc 24. A plurality of such U-shaped flow paths 26 are provided.

  As shown in FIG. 1, a motor 28 that rotates the disc 24 is provided at the center of the disc 24. By connecting the rotating shaft 29 of the motor 28 to the disc 24, the centrifugal force of the disc 24 by the motor 28 is used to perform blood separation to separate blood into plasma and blood cells.

  In Example 1, including Example 2 described later, the disk 24 is formed of an acrylic plate. The material of the circular plate 24 is not limited to acrylic, and any resin optically transparent material such as the above-described PDMS, polycarbonate, COP may be used.

  Further, as shown in FIG. 1, a resin substrate 50 is provided as a liquid dropping auxiliary device in order to reliably drop blood from the nozzle 23 onto the disc 24. The resin substrate 50 corresponds to the liquid dropping auxiliary device in this invention.

  Next, a specific configuration of the resin substrate 50 will be described with reference to FIG. 3 including FIG. FIG. 3A is a schematic plan view of the resin substrate according to the first embodiment, and FIG. 3B is a cross-sectional view taken along the line AA in FIG.

  The resin substrate 50 is annular as shown in the plan view of FIG. The resin substrate 50 is formed of the same acrylic resin as the disk 24 in FIG. The material of the resin substrate 50 is not limited to acrylic, and any resin optically transparent material such as PDMS, polycarbonate, COP may be used. Further, the resin substrate 50 is not necessarily formed of the same material as the disk 24.

  In the first embodiment, the resin substrate 50 has a cylindrical shape having the same diameter as the flow path inlet 25 (see FIG. 2) of the disc 24 in FIG. 2 as shown in the sectional view of FIG. It has a hole 51, and is designed so that the through hole 51 is in communication with the flow path inlet 25 when overlapped with the disk 24. Usually, the number of through holes 51 is the same as the number of the U-shaped flow paths 26 (see FIG. 2), and 36 through holes 51 are provided in FIG. The through hole 51 corresponds to the through hole in this invention.

  In Example 1, as shown in the plan view of FIG. 3B, a film 52 is attached to the surface opposite to the surface in contact with the disk 24 of FIG. 2 corresponding to the container. Since the resin substrate 50 is placed on the upper surface of the disc 24, the surface in contact with the disc 24 is the lower surface and the opposite surface is the upper surface when viewed from the resin substrate 50. Therefore, the film 52 is attached to the upper surface of the resin substrate 50. In the first embodiment, a cellophane tape is used as the film 52. A commercially available product can be substituted for the cellophane tape. As will be apparent from the reason described later, the film 52 is not limited to the cellophane tape as long as the nozzle 13 (see FIG. 1) made of an injection needle is broken (thickness of about 100 μm to several 100 μm). A thin film other than a cellophane tape may be used as exemplified by a film having no adhesiveness. The film 52 corresponds to the thin film in this invention.

  When the resin substrate 50 is placed on the upper surface of the disk 24 in FIG. 2, the disk 24 and the resin substrate 50 are respectively placed so that the through hole 51 and the flow path inlet 25 (see FIG. 2) coincide with each other. After positioning, the disk 24 and the resin substrate 50 are fixed with an adhesive tape or an adhesive.

  The blood cut out at a predetermined volume in the blood collection apparatus 10 is pumped from the main flow path 13 of the blood collection apparatus 10 to the nozzle 23 by gas (for example, air or gas). Before dropping from the nozzle 23, the nozzle 23 is lowered and the film 52 is broken to enter the inside of the through hole 51, and blood is sent out from the nozzle 23 and discharged. The blood dropped from the nozzle 23 falls to the flow path inlet 25, and the blood that foamed during the dropping and could not be dropped to the flow path inlet 25 is also penetrated because the upper surface of the resin substrate 50 is blocked by the film 52. It remains inside the hole 51 and enters the flow path inlet 25. After the blood is dropped, the nozzle 23 is raised and extracted from the disk 24 and the resin substrate 50.

  According to the resin substrate 50 according to the first embodiment, liquid (blood in each embodiment) is supplied from the tip of the discharge unit (the nozzle 23 in each embodiment) toward the liquid dropping auxiliary device (the resin substrate 50 in each embodiment). Before discharging, a container (a disk 24 in each embodiment) having a hole (a flow path inlet 25 in each embodiment) for dropping and collecting a liquid (blood), and the liquid dropping auxiliary device (resin substrate 50). ) Is set so that the hole (flow path inlet 25) matches the through hole 51 of the liquid dropping auxiliary device (resin substrate 50). The liquid dripping auxiliary device (resin substrate 50) is provided with a thin film (film 52 in this embodiment 1) attached to the surface (upper surface) opposite to the surface (lower surface) in contact with the container (disc 24), The liquid (blood) is prevented from leaking out of the liquid dropping auxiliary device (resin substrate 50). When the discharge part (nozzle 23) breaks the thin film (film 52) and the tip of the discharge part (nozzle 23) enters the inside of the through hole 51, liquid (blood) is discharged, and the hole is formed through the through hole 51. Liquid (blood) is dropped to the inlet (flow path inlet 25). The liquid (blood) that foamed during the dropping of the liquid (blood) and could not be dropped into the hole inlet (flow path inlet 25) does not leak out to the outside by the thin film (film 52). Enter the entrance 25) again. As a result, scattering of liquid (blood) due to foaming or the like can be prevented.

  In Example 1, including Example 2 described later, the liquid is blood. Since blood is highly viscous and has a surface-active effect, it can be prevented from being scattered by foaming or the like when applied to blood in the first embodiment.

Next, Embodiment 2 of the present invention will be described with reference to the drawings.
4A is a schematic plan view of a resin substrate according to the second embodiment, FIG. 4B is a cross-sectional view taken along the line AA in FIG. 4A, and FIG. FIG. 4B is a cross-sectional view taken along the line AA of FIG. 4A according to another embodiment different from FIG. Constituent elements common to the first embodiment described above are denoted by the same reference numerals, description thereof is omitted, and illustration is omitted. In addition, the blood collection device and the measurement device according to the second embodiment have the same configuration as that in FIG. 1 described in the first embodiment, and the disk according to the second embodiment is the same as that in FIG. 2 described in the first embodiment. Since it is the same structure, the explanation and illustration are omitted.

  As in the first embodiment, the resin substrate 50 has an annular shape as shown in the plan view of FIG. 4A, and the material of the resin substrate 50 is the same as that in the first embodiment. Omitted. In Example 1, the film 52 (see FIG. 3) was provided, but in Example 2, no film was provided.

  In the second embodiment, the resin substrate 50 has a through hole 51, and the through hole 51 communicates with the flow path inlet 25 (see FIG. 2) when overlapped with the disk 24 of FIG. 2. Designed to be The through holes 51 have the same number (36 in each embodiment) as the U-shaped flow paths 26 (see FIG. 2). The through hole 51 corresponds to the through hole in this invention.

In the second embodiment, the through hole 51, as shown in the sectional view of FIG. 4 (b) or FIG. 4 (c), the surface (lower surface) in contact with disk 24 in FIG. 2 and P _B, contact surfaces ( the opposite surface to the lower surface) P _B (the upper surface) is taken as P _U, the diameter of the lower surface P _B side of the through-hole 51 is designed to be larger than the diameter of the upper face P _U side of the through-holes 51 ing. The diameter of the through hole 51 of the disc 24 in contact with the surface (lower surface) P _B side (see Figure 2) channel inlet 25 the same diameter as or is less. The diameter of the opposite surface (upper surface) P _U of the side through-hole 51 is adapted to nozzle 23 (see FIG. 1) or 2 times the outer diameter of the tip.

In case of FIG. 4 (b), the inner wall surface of the through hole 51 by forming the inclined surface shape, the diameter of the lower surface P _B side of the through-hole 51 is greater than the diameter of the upper face P _U side of the through-holes 51 Forming. In the case of FIG. 4 (c), by forming the inner wall surface of the through-hole 51 in the stepped shape, the diameter of the lower surface P _B side of the through hole 51, than the diameter of the upper face P _U side of the through-holes 51 Is also formed large. There are no particular restrictions on the number of steps or the width of the steps.

  Similar to the first embodiment described above, when the resin substrate 50 is placed on the upper surface of the disk 24 in FIG. 2, the through hole 51 and the flow path inlet 25 (see FIG. 2) are aligned. The disk 24 and the resin substrate 50 are fixed with an adhesive tape or an adhesive and positioned.

  The blood cut out at a predetermined volume in the blood collection apparatus 10 is pumped from the main flow path 13 of the blood collection apparatus 10 to the nozzle 23 by gas (for example, air or gas). Before dropping from the nozzle 23, the nozzle 23 is lowered to enter the inside of the through hole 51, and blood is sent out from the nozzle 23 and discharged from the nozzle 23. After the blood is dropped, the nozzle 23 is raised and extracted from the disk 24 and the resin substrate 50.

According to the resin substrate 50 according to the second embodiment, the liquid (blood in each embodiment) is supplied from the tip of the discharge unit (the nozzle 23 in each embodiment) toward the liquid dropping auxiliary device (the resin substrate 50 in each embodiment). Before discharging, a container (a disk 24 in each embodiment) having a hole (a flow path inlet 25 in each embodiment) for dropping and collecting a liquid (blood), and the liquid dropping auxiliary device (resin substrate 50). ) Is set so that the hole (flow path inlet 25) matches the through hole 51 of the liquid dropping auxiliary device (resin substrate 50). Liquid dripping assist device (in this embodiment 2 diameter) diameter (resin substrate 50) surface in contact with the container (disc 24) in (lower surface) P _B side through hole 51, the contact surface (lower surface) P _B the opposite surface (upper surface) P is _U side is larger than the diameter (diameter) of the through-holes 51, towards the contact surface (lower surface) than P _B of the opposite surface (not in contact towards the face: the upper surface) structure that does not only in enclosed spaces a through hole 51 to reduce the diameter of P _U of the through hole 51 (diameter) becomes possible. Therefore, liquid (blood) is discharged when the tip of the discharge portion (nozzle 23) enters the inside of the through hole 51, and the liquid (blood) reaches the hole inlet (flow path inlet 25) through the through hole 51. When dripping, the escape route of the gas (for example, air) sent with the liquid (blood) can be ensured. Therefore, the liquid (blood) enters the hole entrance (flow path entrance 25) without being blown away by the gas. As a result, scattering of liquid (blood) due to foaming or the like can be prevented.

In the second embodiment, preferably, contact surface (lower surface) diameter of the P _B side of the through-hole 51 (diameter), be less than or equal to the diameter of the container hole of the (disk 24) (channel inlet 25) (diameter) The diameter (diameter) of the through hole 51 on the surface (upper surface) P _U side opposite to the surface (lower surface) P _B in contact with the discharge unit (nozzle 23) that discharges toward the liquid dropping auxiliary device (disk 24). ) Larger than the tip diameter (outer diameter). The diameter of the through hole 51 of the upper surface P _U side more than twice of the outer diameter of the second embodiment in the nozzle 23 is formed. When the liquid (blood) is dropped from the tip of the discharge part (nozzle 23) through the through hole 51 to the inlet of the hole (flow path inlet 25), the diameter gradually increases toward the downstream side. The liquid (blood) can be surely dropped toward the downstream side without the liquid (blood) flowing back or scattering upstream.

  In Example 2, including Example 1 described above, the liquid is blood. Since blood is highly viscous and has a surface-active effect, it can be prevented from being scattered by foaming or the like when applied to blood in the second embodiment.

  The present invention is not limited to the above-described embodiment, and can be modified as follows.

  (1) In each of the embodiments described above, the through-hole is circular and the diameter is the diameter of the circle, but the diameter is not necessarily limited to the diameter or radius of the circle. The length of the short axis or long axis of the elliptical through hole may be the diameter, and the size of the line connecting the diagonals of the rectangular through hole may be the diameter.

  (2) In each of the above-described embodiments, blood has been described as an example of a liquid to be collected. However, as long as the liquid is to be collected, a radioactive substance or a fluorescent agent is not limited to blood. It may be a contained liquid or a mixed solution used in an analyzer. Further, the liquid to be collected may not be the liquid to be centrifuged.

  (3) In each of the above-described embodiments, the container is a disk that performs centrifugation. However, when the liquid to be collected is not the liquid to be centrifuged, the liquid is dropped and collected. If it is a container with a hole, it is not limited to a disk. It may be a square plate or a polygonal plate.

  (4) In each of the above-described embodiments, a plurality of U-shaped flow paths 26 formed in the radial direction are provided by radially grooving along the radial direction of the container (the disk 24 in each embodiment). However, it is not always necessary to arrange them radially. For example, you may arrange | position in parallel mutually. Moreover, it is not limited to a U-shaped path | route, The structure which has only a mere hole may be sufficient.

  (5) In each embodiment described above, the number of through-holes is the same as the number of holes (in each embodiment, the U-shaped flow path 26), but the number is not necessarily the same. For example, each time a liquid is dropped, the hole (flow path inlet 26) may be shared and collected, or conversely, each time a liquid is dropped, the through hole 51 may be shared and dropped.

  (6) In each of the above-described embodiments, the liquid dropping auxiliary device is the annular resin substrate 50, but it is not necessary to be annular. Similarly to the disc 24, the central portion may be flat.

  (7) In each of the above-described embodiments, the liquid dropping auxiliary device is the circular resin substrate 50 according to the same shape as the disk 24 corresponding to the container. However, the liquid dropping auxiliary device has various shapes according to the shape of the container. You may apply and it is not necessary to make it the same shape as the shape of a container.

  (8) In Example 1 described above, the film 52 corresponding to the thin film is attached only to the annular resin substrate 50, but the film 52 may be attached to the center (hollow part).

24 ... disc 25 ... channel inlet 26 ... U-shaped flow path 50 ... a resin substrate 51 ... through hole 52 ... Film P _B ... lower surface P _U ... top

Claims (6)

A liquid dropping auxiliary device used for a container having a hole for dropping and collecting a liquid,
The liquid dropping auxiliary device is
Having a through hole communicating with the hole of the container;
A liquid drip assisting device comprising a thin film attached to a surface opposite to a surface in contact with the container. A liquid dropping auxiliary device used for a container having a hole for dropping and collecting a liquid,
The liquid dropping auxiliary device is
Having a through hole communicating with the hole of the container;
The liquid drip assisting apparatus, wherein a diameter of the through hole on a surface side in contact with the container is larger than a diameter of the through hole on a surface side opposite to the surface in contact with the container. The liquid dropping auxiliary device according to claim 2,
The diameter of the through hole on the surface side to be in contact is not more than the diameter of the hole of the container,
The liquid dripping auxiliary device, wherein a diameter of the through hole on a surface opposite to the surface to be in contact with is larger than a diameter of a tip of a discharge unit that discharges toward the liquid dripping auxiliary device. In the liquid dripping auxiliary | assistance apparatus of Claim 2 or Claim 3,
By forming the inner wall surface of the through-hole into a slope shape, the diameter of the through-hole on the side of the contacting surface is formed larger than the diameter of the through-hole on the surface side opposite to the contacting surface. A liquid drip assisting device. In the liquid dripping auxiliary | assistance apparatus of Claim 2 or Claim 3,
By forming the inner wall surface of the through-hole in a stepped shape, the diameter of the through-hole on the contact surface side is formed larger than the diameter of the through-hole on the surface side opposite to the contact surface. A liquid drip assisting device. In the liquid dripping auxiliary | assistance apparatus in any one of Claims 1-5,
A liquid drip assisting device, wherein the liquid is blood.

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