JP2014200542A - Medical bag and method for using the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a medical bag capable of suppressing physical stimulation to cells when mixing a cell-containing liquid and an action liquid.SOLUTION: A bag body 1 is formed with a division part 9 dividing an inside made hollow in which the inner face projects into a space by making the inside hollow and forming a recessed part 8 in the outer face into a cell storage chamber 2 and an action liquid storage chamber 3. A clip 4 is fastened to the bag body 1 in a position of the division part 9 from the outside such that a cell-containing liquid M stored in the cell storage chamber 2 and an action liquid S stored in the action liquid storage chamber 3 do not respectively flow out, a part of the clip 4 is connected to the inside of the recessed part 8, and the cell-containing liquid M and the action liquid S are mixed except the division part 9 from inside the bag body 1 by moving the clip 4 to pull out the recessed part 8.

Description

  The present invention relates to a medical bag and a method for using the same, and more particularly to a medical bag for mixing a cell-containing liquid and a working liquid and a method for using the same.

  In the medical field, attempts have been made to artificially change cells collected from the human body and the like, and to use the changed cells for treatment or the like. For example, macrophages can be generated by supplying a working fluid that induces differentiation of progenitor cells to macrophage progenitor cells (monocytes). When the generated macrophages are administered to a wound site such as an ulcer, healing of the wound site is promoted, and thus attention has been paid as a highly safe treatment method.

  Here, as an action liquid that induces differentiation into macrophages, for example, those that give physical stimulation such as osmotic shock to progenitor cells, or those that contain inducers such as cytokines have been reported. When osmotic shock is applied, a cell-containing solution containing macrophage precursor cells and a working solution are contained in separate medical bags, and the working solution contains cells via a tube connecting the medical bags. It is injected into a medical bag containing the liquid. The injected working fluid is mixed with the cell-containing fluid in the medical bag, and macrophages can be generated by applying osmotic shock to the progenitor cells.

However, in general, cells are very vulnerable to external stimuli such as physical stimuli, and even when the working fluid is supplied to a medical bag containing a cell-containing fluid, the physical stimulation when the working fluid is poured into the bag. As a result, more progenitor cells are killed and the number of macrophages generated is reduced. For this reason, there is a need for a medical bag that can suppress physical stimulation received by cells when the cell-containing liquid and the working liquid are mixed.
On the other hand, various methods for mixing two types of solutions not containing cells have been proposed. For example, in Patent Document 1, an infusion bag divided into two chambers is packaged in an exterior bag, an amino acid-containing preparation is filled in one chamber of the infusion bag, and physiological saline is filled in the other chamber. An infusion bag package is disclosed.

Japanese translation of PCT publication No. 2004-121649

  Each of the two chambers of the infusion bag is provided with a port, and a dispensing process such as injecting a vitamin with a syringe into a chamber filled with physiological saline, for example, is performed through the port. However, since such an infusion bag is not intended for cells that are vulnerable to physical stimulation, such as cells, it is not considered to suppress physical stimulation when mixing liquid, and as described above, cell-containing It is difficult to apply to a medical bag in which a liquid and a working liquid are mixed.

  The present invention has been made to solve such conventional problems, and a medical bag capable of suppressing physical stimulation to cells when mixing a cell-containing solution and a working solution and a method of using the same The purpose is to provide.

  The medical bag according to the present invention has a partitioning portion that makes the interior hollow and forms a recess on the outer surface so that the inner surface protrudes into the space, thereby dividing the hollow interior into a cell storage chamber and a working fluid storage chamber. And a clip that holds the bag body from the outside at the position of the partitioning portion so that the cell-containing liquid stored in the cell storage chamber and the working liquid stored in the working fluid storage chamber do not flow out, respectively. A part of the clip is connected to the recess, and the clip is moved so as to pull out the recess, thereby mixing the cell-containing liquid and the working liquid from the bag body except for the sorting part.

Here, the section is preferably formed so as to cross the bag body in one direction.
In addition, the clip has an insertion part for insertion into the recess, and the tip of the insertion part is bonded to the innermost part of the recess so that the recess can be pulled out together with the insertion part as the clip moves. . In addition, the clip has a three-pronged shape, and the two fastening portions arranged on the outside can fasten the bag main body, and the insertion portion can be placed between the two fastening portions. Further, the two fastening portions can be configured so as to surround each other around the bag body, and the other fastening portion can move with respect to one fastening portion. The clip may further include an adjustment unit for adjusting the interval between the two fastening portions.

In addition, a holding portion whose inner surface is recessed outward is formed inside the bag body, and the sorting portion can cross the bag body in one direction and can hold the tip portion in the holding portion.
Further, by forming the first concave portion and the second concave portion on the outer surface of the bag body, a first partition portion and a second partition portion for partitioning the inside of the bag body are formed, and the position of the first partition portion is determined. It is preferable to have the 1st clip which fastens a bag body from the outside, and the 2nd clip which fastens a bag body from the outside in the position of the 2nd division part.

Further, the first partitioning portion and the second partitioning portion are partitioned so that the first working fluid storage chamber is disposed between the cell storage chamber and the second working fluid storage chamber in the bag body 1. The first working fluid can be stored in the first working fluid storage chamber and the second working fluid can be stored in the second working fluid storage chamber.
In addition, the first partitioning portion and the second partitioning portion are partitioned so that the first working fluid storage chamber and the second working fluid storage chamber are respectively disposed adjacent to the cell storage chamber in the bag body. The first working fluid can be stored in the first working fluid storage chamber and the second working fluid can be stored in the second working fluid storage chamber.

  A method for using a medical bag according to the present invention is a method for using a medical bag in which a cell-containing liquid and a working liquid are mixed using the medical bag described above, wherein the dividing portion is formed in the bag body. By fastening the clip from the outside of the bag body at the position, a cell housing chamber and a working fluid housing chamber separated from each other are formed, and the cell containing fluid is housed in the cell housing chamber and the working fluid is housed in the working fluid housing chamber. Then, by moving the clip so as to pull out the recess of the bag main body, the cell-containing liquid and the working liquid are mixed from the bag main body except for the sorting part.

  Here, after the sorting part is removed from the bag body, the working fluid stored in the working fluid storage chamber is allowed to flow into the cell storage chamber, and the clip is moved so as to form a recess again on the outer surface of the bag body. The dividing chamber is formed in the bag body, and the mixing chamber is formed at the position where the cell storage chamber is formed by fastening the clip from the outside of the bag body at the position of the dividing portion. It is preferable to mix the cell-containing solution and the working solution.

  According to the present invention, the cell-containing liquid and the working liquid are mixed because the cell-containing liquid and the working liquid are mixed by removing the section that separates the cell housing chamber and the working liquid-containing chamber by moving the clip. In this case, it becomes possible to suppress physical stimulation to the cells.

It is a top view which shows the structure of the medical bag which concerns on Embodiment 1 of this invention. FIG. 2A is a perspective view showing a bag main body in a separated state, and FIG. 2B is a perspective view showing the bag main body in an opened state, showing the configuration of the bag main body of the medical bag according to the first embodiment. . The structure of the clip of the medical bag which concerns on Embodiment 1 is shown, FIG. 3a is sectional drawing which shows the state which the clip fitted, and FIG. 3b is sectional drawing which shows the state which cancel | released fitting of the clip. FIG. 4A is a plan view of the medical bag, and FIG. 4B is a cross-sectional view taken along line AA of FIG. 4A. FIG. 5A is a plan view of the medical bag, and FIG. 5B is a cross-sectional view taken along the line BB of FIG. 5A. FIG. 6A is a plan view of the medical bag, and FIG. 6B is a cross-sectional view taken along the line CC of FIG. 6A. It is a figure which shows a mode that the mixing chamber of the medical bag which concerns on Embodiment 1 was formed narrowly. FIG. 6 is a cross-sectional view showing a configuration of a clip of a medical bag according to a modification of the first embodiment. FIG. 10 is a cross-sectional view showing a configuration of a clip of a medical bag according to another modification of the first embodiment. FIG. 10 is a plan view showing a configuration of a medical bag according to still another modification of the first embodiment. 6 is a plan view showing the configuration of a medical bag according to Embodiment 2. FIG. 10 is a plan view showing a configuration of a medical bag according to a modification of the second embodiment. FIG. FIG. 12 is a plan view showing a configuration of a medical bag according to another modification of the second embodiment.

Embodiments of the present invention will be described below with reference to the accompanying drawings.
Embodiment 1
FIG. 1 shows a medical bag according to Embodiment 1 of the present invention. This medical bag has a bag body 1 having a hollow inside, and a clip 4 that divides the bag body 1 into a cell storage chamber 2 and a working fluid storage chamber 3 by sandwiching the bag body 1 from outside. .
Here, the cell accommodating chamber 2 is for accommodating the cell-containing liquid M containing cells, and the working fluid accommodating chamber 3 is for accommodating the working fluid S that acts on the cells. As the cell-containing solution M, for example, blood collected from a human body, a buffy coat obtained by centrifuging blood, or a fraction obtained by fractionating white blood cells can be used. As the working fluid S, for example, a solution containing a cytokine that induces differentiation into macrophages, or distilled water that gives an osmotic shock to cells can be used.

The bag body 1 is provided with a cell injection port 5a and a cell discharge port 5b that communicate with the cell storage chamber 2 formed therein, and a working fluid injection port 6 that communicates with the working fluid storage chamber 3. Is provided. The cell-containing liquid M is injected into the cell storage chamber 2 through the cell injection port 5a, or the solution in the cell storage chamber 2 is discharged through the cell discharge port 5b. On the other hand, the working fluid S is injected into the working fluid storage chamber 3 through the working fluid injection port 6. Further, the cells 7a and 7b can be connected to the cell injection port 5a and the cell discharge port 5b, respectively, and the cell-containing solution M can be accommodated by connecting a syringe or the like to the tip of the tubes 7a and 7b. It can be injected into the chamber 2.
The bag body 1 is made of a soft material that can be easily deformed, and can be made of, for example, polyvinyl chloride. Moreover, the clip 4 can be comprised from a hard material, for example, can be comprised from a plastics etc.

2a and 2b show the configuration of the bag body 1 in detail.
As shown in FIG. 2a, the bag body 1 has a rectangular parallelepiped shape. By forming a recess 8 on the outer surface, the inner surface protrudes into the internal space, and the hollow interior acts as the cell storage chamber 2. A sorting section 9 is formed to divide the liquid storage chamber 3.
The concave portion 8 is formed so as to extend from one side portion 10 toward the other side portion 11 with respect to the two opposite side portions 10 and 11 of the bag body 1, and has an opening on the side portion 10 side. 12 and the innermost portion 13 on the side portion 11 side. Thereby, the sorting part 9 is formed so as to extend from the side part 10 toward the side part 11, and the tip part thereof is disposed in contact with the inner surface of the side part 11. That is, the sorting part 9 is formed so as to cross the inside of the bag body 1 in one direction from the side part 10 toward the side part 11.

Furthermore, since the bag main body 1 is comprised from the soft material, the recessed part 8 can be pulled out outside. As shown in FIG. 2 b, by completely pulling out the recess 8 from the bag body 1, the sorting part 9 is removed from the inside of the bag body 1, and the cell storage chamber 2 and the working fluid separated by the sorting part 9. The storage chamber 3 is integrated, and a mixing chamber 14 for mixing the cell-containing liquid M and the working liquid S is formed inside the bag body 1.
As described above, the bag body 1 is divided into the cell storage chamber 2 and the working fluid storage chamber 3 by the partitioning portion 9, and the cell storage chamber 2 and the working fluid storage chamber 3 are opened except for the partitioning portion 9. The open state can be changed.

3a and 3b show the structure of the clip 4 in detail.
FIG. 3a shows the clip 4 attached to the bag body 1 in a segmented state. The clip 4 has a three-pronged shape, and two fastening portions 15 and 16 for fastening the bag body 1 between them are arranged on the outside, and an insertion portion 17 is arranged between the two fastening portions 15 and 16. Has been.

The insertion portion 17 has a shape protruding in one direction, and is inserted into the recess 8 from the opening 12 of the bag body 1, and its distal end is interposed between the innermost portion 13 of the recess 8 and the adhesive 18. It is glued. The two fastening portions 15 and 16 are formed so as to surround the bag body 1 with each other. That is, the two fastening portions 15 and 16 are arranged so as to surround the bag body 1 at the position of the dividing portion 9.
Further, a fitting portion 19 that is elastically fitted is provided at the tip of the two fastening portions 15 and 16, and the two fastening portions 15 and 16 are fitted to each other by the fitting portion 19. Thereby, the bag main body 1 is clamped from the outside at the position of the dividing portion 9, and the cell storage chamber 2 and the working fluid storage chamber 3 in the bag main body 1 can be completely separated. Thereby, the cell-containing liquid M and the working fluid S can be accommodated in the cell accommodating chamber 2 and the working fluid accommodating chamber 3 without causing them to flow out, respectively.

  In addition, a movable portion 20 is provided in the fastening portion 15. As shown in FIG. 3 b, when the fitting of the two fastening parts 15 and 16 by the fitting part 19 is released, the clip 4 can be opened while the fastening part 15 is deformed by the movable part 20. . Further, when the fitting portions 19 of the two fastening portions 15 and 16 are fitted, the clip 4 can be fastened while the fastening portion 15 is deformed by the movable portion 20.

Next, the operation of the first embodiment will be described.
First, a partitioning portion 9 is formed in the bag body 1 so that the bag body 1 is in a partitioned state, and the bag body 1 is sandwiched between clips 4 at the position of the partitioning portion 9 as shown in FIG. A cell storage chamber 2 and a working fluid storage chamber 3 separated from each other are formed in the main body 1.
Subsequently, a syringe is attached to the tip of the tube 7 a connected to the cell injection port 5 a of the bag body 1, and the cell-containing liquid M filled in the syringe is gently injected into the cell storage chamber 2. On the other hand, the working fluid S is injected into the working fluid storage chamber 3 of the bag body 1 through the working fluid injection port 6.

At this time, the bag body 1 is divided by the dividing portion 9 inside and is fastened by the clip 4 from the outside. If the bag main body 1 is simply fastened with the clip 4 from the outside without forming the dividing portion 9 in the bag main body 1, the cell-containing liquid M injected into the cell storage chamber 2 and the working fluid storage chamber 3 and the action are respectively provided. Although the liquid S may flow out, in the present invention, the cell-containing liquid M is formed because the bag body 1 is further fastened with the clip 4 from the outside after the dividing portion 9 is formed inside the bag body 1. And the working fluid S can be reliably trapped in the cell housing chamber 2 and the working fluid housing chamber 3, respectively, and can be stored in a stable state.
Here, a buffy coat obtained by centrifuging blood collected from a human body is used as the cell-containing liquid M. This buffy coat contains leukocytes, platelets, and the like, and monocytes that are precursor cells of macrophages. The working fluid S contains cytokines and has an action of inducing differentiation of macrophage precursor cells.

  When generating macrophages, first, the fitting of the clip 4 by the fitting part 19 is released, and the clip part 16 is rotated so as to open the clip part 15, as shown in FIGS. 4 a and 4 b. Open the clip 4 completely. At this time, the fastening portion 15 can be rotated while being deformed by the movable portion 20, and the clip 4 can be easily opened. Even when the clip 4 is opened, the cell containing liquid M and the working fluid S are almost discharged from the cell housing chamber 2 and the working fluid housing chamber 3 because the section 9 is formed in the bag body 1. It is kept in a housed state.

Subsequently, as shown in FIGS. 5 a and 5 b, the clip 4 is moved outward along the recess 8 so that the insertion portion 17 of the clip 4 is pulled out from the recess 8 of the bag body 1. Since the distal end portion of the insertion portion 17 is bonded to the innermost portion 13 of the concave portion 8 via the adhesive 18, the concave portion 8 is formed together with the insertion portion 17 as the clip 4 moves, as shown in FIG. The bag body 1 is pulled out.
6a and 6b, when the recess 8 is completely pulled out from the bag body 1, the section 9 is removed from the bag body 1, and the bag body 1 has the cell storage chamber 2 and the working fluid storage chamber. 3 is opened. Thus, a mixing chamber 14 in which the cell storage chamber 2 and the working fluid storage chamber 3 are integrated is formed in the bag body 1, and for example, the cell storage chamber 2 is positioned below the working fluid storage chamber 3. When the bag body 1 is inclined, the working fluid S stored in the working fluid storage chamber 3 is gently introduced into the cell storage chamber 2 along the bottom surface of the mixing chamber 14 to supply the working fluid S. The physical stimulus given to the cell can be suppressed. Moreover, since the clip 4 and the recessed part 8 are connected, the dividing part 9 can be removed from the bag body 1 at a time only by moving the clip 4, and the mixing of the cell-containing liquid M and the working liquid S can be performed quickly. It can be carried out. That is, the cell-containing liquid M and the working liquid S can be rapidly mixed while suppressing physical stimulation given to the cells by removing the sorting part 9 from the bag body 1.

  Thus, by mixing the cell-containing solution M and the working fluid S in the mixing chamber 14 formed in the bag body 1, the working fluid S contains the precursor cells of macrophages contained in the cell-containing fluid M. Can be activated to promote the differentiation of progenitor cells and generate macrophages.

The generated macrophages are taken out from the mixing chamber 14 through the cell discharge port 5b of the bag body 1. Here, since the cell-containing liquid M includes cells other than the precursors of macrophages, it is preferable to isolate and take out only macrophages from the mixed solution K obtained by mixing the cell-containing liquid M and the working liquid S. For example, since the macrophages have a property of adsorbing to the inner surface of the bag body 1, the mixed solution K not containing macrophages can be taken out from the mixing chamber 14 in a state where the macrophages are adsorbed to the bag body 1. Then, a macrophage is isolated by supplying the peeling liquid which peels a macrophage from the inner surface of the bag main body 1 in the mixing chamber 14, peeling the macrophage adsorbed to the bag main body 1, and taking out from the mixing chamber 14. be able to.
The macrophages taken out from the mixing chamber 14 in the bag body 1 are administered to a wound site such as an ulcer and used for treatment of the wound site.

  According to the present embodiment, since the cell-containing liquid M and the working liquid S are mixed at a time only by moving the clip 4, the mixing operation can be performed quickly. Furthermore, since the cell-containing liquid M and the working liquid S can be mixed gently, the cells can be prevented from being killed by physical stimulation, and macrophages can be efficiently generated.

The mixing chamber 14 for mixing the cell-containing liquid M and the working liquid S is preferably formed narrow. For example, the total amount of the cell-containing liquid M and the working liquid S is adjusted so as to be accommodated in the cell storage chamber 2, and the cell-containing liquid M and the working liquid S act on the cell storage chamber 2 in the bag body 1. The liquid is stored in the liquid storage chamber 3. Subsequently, after the clip 4 is moved to remove the sorting portion 9 from the bag body 1 and the working fluid S stored in the working fluid storage chamber 3 flows into the cell storage chamber 2 side, as shown in FIG. Then, the insertion portion 17 of the clip 4 is moved so that the concave portion 8 is formed again on the outer surface of the bag body 1. As a result, the dividing portion 9 is formed again in the bag body 1, and the mixing chamber K is formed at the position where the cell storage chamber 2 is formed, and the cell-containing liquid M and the working liquid are contained in the mixing chamber K. The liquid mixture 21 which mixed S is accommodated. Subsequently, the clip 4 is clamped from the outside of the bag body 1 at the position of the dividing portion 9, whereby a mixing chamber K in which the mixed solution 21 is confined is formed in the bag body 1. Can be generated.
Thus, by forming the mixing chamber K narrow, loss at the time of taking out the liquid mixture 21 can be suppressed, and the recovery rate of the generated macrophages can be improved.

Further, as shown in FIG. 8, the clip 4 can be provided with an adjusting portion 21 that adjusts the interval between the two fastening portions 15 and 16. The adjustment part 21 is formed to be extendable and contractable to such an extent that the cell-containing liquid M and the working liquid S accommodated in the bag body 1 do not flow out, and the interval between the fastening parts 15 and 16 is set to the thickness of the bag body 1. Can be adjusted accordingly.
Further, in the above embodiment, the movable portion 20 is provided only in the fastening portion 15 of the clip 4 so that the fastening portion 15 can be rotated with respect to the fastening portion 16. However, as shown in FIG. 16 can also be provided with a movable part 22. Thereby, both the fastening part 15 and the fastening part 16 can be rotated, and the clip 4 can be open | released or fastened.

Further, as shown in FIG. 10, a holding portion 23 whose inner surface is recessed outward can be formed inside the bag body 1. Here, the sorting part 24 is formed so as to cross the bag body 1 in one direction, and the tip part is held in the holding part 23. By holding the tip of the sorting part 24 in the holding part 23, the posture of the sorting part 24 is reliably maintained, and the cell-containing liquid M and the working liquid contained in the cell containing chamber 2 and the working fluid containing chamber 3. The outflow of S can be further suppressed. The holding portion 23 also serves as a mark when the clip 4 is moved. For example, even when the bag body 1 is repeatedly changed between the partitioned state and the open state, the cell storage chamber 2 and the working fluid storage chamber 3 are kept to a certain size. Can be formed.
Further, in the above embodiment, the sorting portion 9 is formed so as to extend from one side portion 10 toward the other side portion 11 with respect to the two opposite side portions 10 and 11 of the bag body 1. However, the present invention is not limited to this as long as the inside of the bag body 1 can be divided. For example, a section that extends in a direction orthogonal to the section 9 can be formed.

Embodiment 2
In the first embodiment, one cell storage chamber 2 and one working fluid storage chamber 3 are formed in the bag body 1, but when a plurality of working fluids S are mixed with the cell-containing solution M, A plurality of storage chambers corresponding to the mixing method can be formed in the bag body 1.
For example, by forming a first recess and a second recess on the outer surface of the bag body 1, a first partition portion and a second partition portion for partitioning the interior of the bag body 1 are formed, and the first clip is used. The bag body 1 is fastened from the outside at the position of the first section, and the bag body 1 is fastened from the outside at the position of the second section by the second clip, so that three storage chambers are formed in the bag body 1. Can be formed.

  Specifically, as shown in FIG. 11, in the bag body 1, the first working fluid storage chamber 31 is arranged between the cell storage chamber 2 and the second working fluid storage chamber 32. The partitioning portion 33 and the second partitioning portion 34 can be formed. The cell storage chamber 2 and the first working fluid storage chamber 31 partitioned by the first partitioning portion 31 are reliably secured by fastening the bag body 1 from the outside at the position of the first partitioning portion 31 by the first clip 35. Divided into In addition, the first working fluid storage chamber 31 and the second working fluid storage chamber 32 separated by the second partitioning portion 34 are connected to the bag body 1 at the position of the second partitioning portion 34 by the second clip 36. It is surely divided by fastening from the outside. The cell-containing chamber 2 contains a cell-containing liquid M, and the first working fluid-containing chamber 31 and the second working fluid-containing chamber 32 contain a first working fluid Sa and a second working fluid Sb, respectively. Be contained.

  For example, when it is necessary to mix the first working liquid Sa and the second working liquid Sb before supplying the working liquid to the cell-containing liquid M, the second sorting section is firstly provided from the bag body 1. By removing 34, the first working fluid Sa and the second working fluid Sb are mixed. Subsequently, by removing the first sorting part 33 from the bag body 1, the first mixed liquid of the first working liquid Sa and the second working liquid Sb can be mixed with the cell-containing liquid M. it can.

  According to the present embodiment, since a plurality of storage chambers are formed in the bag body 1, the cell-containing liquid M and the working liquid can be mixed in a plurality of stages, and the mixing method is complicated. But we can respond.

In addition, a 1st division part and a 2nd division part can also be formed so that a 1st working fluid storage chamber and a 2nd working fluid storage chamber may be arrange | positioned adjacent to a cell storage chamber, respectively.
For example, as shown in FIG. 12, a part of the bag body 1 is formed so as to be divided into two parts, and a first partitioning portion 37 is formed at one root portion thereof to form the cell storage chamber 2 and the first working fluid. The storage chamber 38 is partitioned, and a second partitioning portion 39 is formed at the other root portion to partition the cell storage chamber 2 and the second working fluid storage chamber 40. The partitioned cell storage chamber 2 and the first working fluid storage chamber 38 are reliably separated by fastening the bag body 1 from the outside at the position of the first partition portion 37 by the first clip 41. In addition, the cell storage chamber 2 and the second working fluid storage chamber 40 are reliably separated by fastening the bag body 1 from the outside at the position of the second partitioning portion 39 by the second clip 42. The cell containing chamber 2 contains the cell-containing liquid M, and the first working fluid containing chamber 38 and the second working fluid containing chamber 40 contain the first working fluid Sa and the second working fluid Sb, respectively. can do.

  Further, as shown in FIG. 13, the bag body 1 is formed in an L shape, and the first partitioning portion 37 is formed so as to dispose the first working fluid storage chamber 38 adjacent to the cell storage chamber 2. In addition, the second section 39 can be formed so that the second working fluid storage chamber 40 is disposed adjacent to the cell storage chamber 2. The first clip 41 is fastened at the position of the first partition part 37, and the second clip 42 is fastened at the position of the second partition part 39. Then, the cell-containing liquid M is stored in the cell storage chamber 2, and the first working liquid Sa and the second working liquid Sb are respectively stored in the first working liquid storage chamber 38 and the second working liquid storage chamber 40. Be contained.

  In this way, by arranging the first working fluid storage chamber 38 and the second working fluid storage chamber 40 so as to be adjacent to the cell housing chamber 2, for example, the cell-containing fluid M and the first working fluid Sa can be obtained. After mixing, the second working fluid Sb can be further mixed. As an example, a cell-containing solution M containing macrophage progenitor cells is stored in the cell storage chamber 2, and a first working solution Sa (for example, distilled water) that applies osmotic shock to the progenitor cells is used as the first working solution. The second working fluid Sb (for example, physiological saline) that can be housed in the housing chamber 38 and returns the osmotic pressure change caused by the first working fluid Sa can be housed in the second working fluid housing chamber 40. Thus, by removing the first section 37 from within the bag body 1, an osmotic shock is applied to the precursor cells of macrophages contained in the cell-containing liquid M, and when the precursor cells are differentiated into macrophages, By removing the second section 39 from the osmotic pressure, the osmotic pressure can be restored and the generated macrophages can be stabilized.

  DESCRIPTION OF SYMBOLS 1 Bag main body, 2 cell storage chamber, 3 working fluid storage chamber, 4 clip, 5a cell injection port, 5b cell discharge port, 6 working fluid injection port, 7a, 7b tube, 8 recessed part, 9, 24 division part 10, 11 side part, 12 opening part, 13 innermost part, 14 mixing chamber, 15, 16 fastening part, 17 insertion part, 18 adhesive, 19 fitting part, 20, 22 movable part, 21 adjustment part, 23 Holding portion, 31, 38 First working fluid storage chamber, 32, 40 Second working fluid storage chamber, 33, 37 First partitioning portion, 34, 39 Second partitioning portion, 35, 41 First clip , 36, 42 Second clip, M cell-containing solution, S working solution, K mixed solution, Sa first working solution, Sb second working solution.

Claims (12)

The bag body in which the inner surface protrudes into the space by forming a concave portion on the outer surface while making the inside hollow, and a partitioning portion is formed to divide the hollow interior into a cell storage chamber and a working fluid storage chamber,
A clip for fastening the bag body from the outside at the position of the partitioning portion so that the cell-containing liquid stored in the cell storage chamber and the working liquid stored in the working fluid storage chamber do not flow out, respectively.
A part of the clip is connected to the concave portion, and the cell-containing liquid and the working liquid are mixed by removing the section from the bag body by moving the clip so as to pull out the concave portion. Medical bag.   The medical bag according to claim 1, wherein the section is formed so as to cross the bag body in one direction.   The clip has an insertion portion for insertion into the recess, and the distal end portion of the insertion portion is bonded to the innermost portion of the recess, so that the recess along with the insertion portion is moved along with the movement of the clip. The medical bag according to claim 1 or 2, wherein is drawn out.   The clip has a three-pronged shape, and two fastening portions arranged on the outside fasten with the bag body interposed therebetween, and the insertion portion is placed between the two fastening portions. A medical bag according to 1.   5. The medical use according to claim 4, wherein the two fastening portions have a shape surrounding each other around the bag body, and the other fastening portion is configured to be movable with respect to one fastening portion. bag.   The medical bag according to claim 4 or 5, wherein the clip further includes an adjustment portion for adjusting an interval between the two fastening portions. Inside the bag body, a holding portion whose inner surface is recessed outward is formed,
The medical bag according to any one of claims 1 to 6, wherein the sorting portion traverses the bag body in one direction and a tip portion is held in the holding portion. Forming a first recess and a second recess on the outer surface of the bag body to form a first partition portion and a second partition portion for partitioning the bag body;
The first clip for fastening the bag body from the outside at the position of the first partitioning portion, and the second clip for fastening the bag body from the outside at the position of the second partitioning portion. The medical bag as described in any one of these.   The first sorting section and the second sorting section are arranged such that the first working fluid storage chamber is disposed between the cell housing chamber and the second working fluid storage chamber in the bag body 1. The medical bag according to claim 8, wherein the first working fluid is stored in the first working fluid storage chamber and the second working fluid is stored in the second working fluid storage chamber.   In the bag main body, the first working fluid storage chamber and the second working fluid storage chamber are respectively disposed adjacent to the cell housing chamber in the bag body. The medical bag according to claim 8, wherein the first working fluid is stored in the first working fluid storage chamber and the second working fluid is stored in the second working fluid storage chamber. A method for using a medical bag in which a cell-containing liquid and an action liquid are mixed using the medical bag according to any one of claims 1 to 10,
By fastening the clip from the outside of the bag body at the position of the partition formed in the bag body, a cell storage chamber and a working fluid storage chamber separated from each other are formed,
Storing the cell-containing liquid in the cell storage chamber and storing the working fluid in the working fluid storage chamber;
A method for using a medical bag, wherein the cell-containing liquid and the working liquid are mixed by moving the clip so as to pull out the concave portion of the bag body to remove the sorting portion from the bag body. After the partition portion is removed from the bag body, the working fluid stored in the working fluid storage chamber is caused to flow into the cell storage chamber side,
The clip is moved so as to form the recess again on the outer surface of the bag body, and the partitioning portion is formed in the bag body,
By fastening the clip from the outside of the bag body at the position of the dividing portion, a mixing chamber is formed at the position where the cell storage chamber was formed,
The method for using a medical bag according to claim 11, wherein the cell-containing liquid and the working liquid are mixed in the formed mixing chamber.

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