JP2014157059A - Analysis container - Google Patents

Abstract

PROBLEM TO BE SOLVED: To improve analysis accuracy of an analysis device for a gene or the like.SOLUTION: An analysis container includes a body casing 21, a rotary shaft insertion hole 5a which is provided at the center of the body casing 21 and in which a revolving shaft is inserted, a reaction part 26 provided on a periphery in the body casing 21 centering on the rotary shaft insertion hole 5a, a suction port provided in an inner circumference side of the reaction part 26, and a discharge port provided in an outer circumference side of the reaction part 26.

Description

  The present invention relates to an analysis container used for analysis of genes and the like, for example.

  This type of analysis container of the related art has a main body case, a rotation shaft insertion hole into which a rotation shaft provided in the center of the main body case is inserted, and a reaction provided on a circumference around the rotation shaft insertion hole. And a configuration provided with a section.

  Directly apply warm air heated by heating means to this analysis container to control the temperature of this analysis container part to the target temperature, thereby preparing the analysis environment by bringing the temperature of the reaction part closer to the target temperature. The analysis accuracy is being improved (Patent Document 1 below).

JP-A-10-2875

  The problem with the conventional example is that the analysis accuracy is low.

  That is, in the above conventional example, the structure is such that the warm air from the blower fan is blown directly onto the surface of the main body case of the analysis container, but since this main body case has a heat capacity, The temperature of the reaction part never coincided, and as a result, the temperature of the reaction part is greatly different from the target temperature, and as a result, the analysis accuracy is lowered.

  Accordingly, the present invention aims to increase the analysis accuracy.

  In order to achieve this object, the present invention provides a main body case, a rotary shaft insertion hole provided in the center of the main body case, into which the rotary shaft is inserted, and the main body case with the rotary shaft insertion hole as a center. The reaction part provided on the circumference | surroundings of this, The suction inlet provided in the inner peripheral side of this reaction part, and the discharge outlet provided in the outer peripheral side of the said reaction part were provided.

  This achieves the intended purpose.

  As described above, the present invention provides a main body case, a rotary shaft insertion hole that is provided at the center of the main body case and into which the rotation shaft is inserted, and a circumference within the main body case with the rotation shaft insertion hole as a center. The analysis part can be improved because the reaction part provided on the inner side of the reaction part, the suction port provided on the inner peripheral side of the reaction part, and the outlet provided on the outer peripheral side of the reaction part are provided.

  That is, in the present invention, since the suction port is provided inside the reaction portion of the main body case and the discharge port is provided outside the reaction portion, the main case is rotated by the rotation shaft, so that the negative shaft generated in the rotation shaft portion. The heated air is sucked in by pressure, and then this heated air is sucked into the main body case from the suction port, then passes through the outer periphery of the reaction part, and is then discharged out of the main body case from the discharge port. . That is, in the present invention, the temperature-controlled heated air passes directly through the outer periphery of the reaction part, so that the temperature of the reaction part can be maintained at the set temperature, and as a result, the analysis accuracy is improved. Can do it.

The perspective view of the analyzer which shows one Embodiment of this invention The perspective view of the analyzer which shows one Embodiment of this invention The top view of the analyzer which shows one Embodiment of this invention The perspective view of the analyzer which shows one Embodiment of this invention The top view of the analyzer which shows one Embodiment of this invention The disassembled perspective view of the analyzer which shows one Embodiment of this invention The perspective view of the analysis container used for the analyzer which shows one Embodiment of this invention The perspective view of the principal part of the analyzer which shows one Embodiment of this invention The partially cutaway perspective view of the principal part of the analyzer which shows one Embodiment of this invention Sectional drawing of the principal part of the analyzer which shows one Embodiment of this invention Sectional drawing of the principal part of the analyzer which shows one Embodiment of this invention Sectional drawing of the principal part of the analyzer which shows one Embodiment of this invention Sectional drawing of the principal part of the analyzer which shows one Embodiment of this invention The top view of the principal part of the analyzer which shows one Embodiment of this invention Sectional drawing of the principal part of the analyzer which shows one Embodiment of this invention Control block diagram of analysis apparatus showing one embodiment of the present invention The figure which shows operation | movement of the analyzer which shows one Embodiment of this invention. The top view of the principal part of the analysis container which shows one Embodiment of this invention Sectional drawing of the principal part of the analysis container which shows one Embodiment of this invention The perspective view of the principal part of the analysis container which shows one Embodiment of this invention The top view of the principal part of the analysis container which shows one Embodiment of this invention The top view of the principal part of the analysis container which shows one Embodiment of this invention, an enlarged view The perspective view of the principal part of the analyzer which shows one Embodiment of this invention The top view of the principal part of the analyzer which shows one Embodiment of this invention, a side view, sectional drawing The disassembled perspective view of the principal part of the analyzer which shows one Embodiment of this invention Sectional drawing of the principal part of the analyzer which shows one Embodiment of this invention Process drawing of analysis apparatus showing one embodiment of the present invention The flowchart of the analyzer which shows one Embodiment of this invention Explanatory drawing of the principal part of the analyzer which shows one Embodiment of this invention

Hereinafter, the thing which applied one Embodiment of this invention to the gene-analysis apparatus is demonstrated using an accompanying drawing.
(Embodiment 1)
1 to 6, reference numeral 1 denotes a main body case, and an opening 3 for allowing the analysis container carry-in tray 2 to appear and disappear is provided on the front surface of the main body case 1.

  That is, FIG. 1 shows a state in which the analysis container carry-in tray 2 is inserted into the main body case 1 from the opening 3, and FIG. 2 shows a state in which the analysis container carry-in tray 2 is pulled out of the main body case 1 from the opening 3. Show. As shown in FIGS. 2, 3 to 4, and 5, the analysis container carry-in tray 2 is loaded with the analysis container 4 shown in FIG. 7 on the upper surface, and then the analysis container 4 is placed in the main body case 1. It works to convey.

  The analysis container 4 is used for gene analysis, for example, and injects a specimen from the opening 5 shown in FIG. 7, and then covers the opening 5 with a lid 20 in that state. 5 is set on the analysis container carry-in tray 2 as shown in FIG. The analysis container 4 will be described in detail later. The sample injected from the opening 5 is branched into a plurality of locations by branching paths, reacted with reagents in each portion, and gene analysis is performed depending on the reaction status. Is to do.

  An analysis chamber 6 shown in FIGS. 8 to 15 is provided on the back side of the opening 3 of the main body case 1, and the analysis container carry-in tray 2 is arranged in this analysis chamber as shown in FIGS. 10 to 13. It is configured to be inserted into 6 or pulled out from this analysis chamber 6. Specifically, as shown in FIG. 15, the analysis chamber 6 connects a small circle portion 6 a that houses the blower fan 7 and a large circle portion 6 b that houses the analysis container rotation driving means 8 shown in FIGS. 10 to 13. It has become the composition.

  An analysis container insertion opening 6 c is provided in the great circle portion 6 b of the analysis chamber 6, and the analysis container carry-in tray 2 can be moved into and out of the analysis chamber 6 from the analysis container insertion opening 6 c.

  In addition, in the great circle portion 6b of the analysis chamber 6, an analysis container rotation drive means 8 is provided. The analysis container rotation drive means 8 includes a bearing 9 that rotatably supports the analysis container 4, As shown in FIGS. 10 to 13, the bearing 9 is lifted upward when the analysis container carry-in tray 2 is inserted into the analysis chamber 6 from the analysis container insertion opening 6 c, and as a result, the analysis container 4 rotates. By supporting the shaft insertion hole 5a, the analysis container 4 is coupled to the rotary drive shaft 10 provided above the analysis chamber 6. Further, since the rotational drive shaft 10 is connected to the motor 11, in the state of FIG. 13, the analysis container 4 is pivotally supported while being sandwiched between the rotational drive shaft 10 and the bearing 9. It can be rotated by.

  On the other hand, a blower fan 7 is provided in the small circle portion 6 a of the analysis chamber 6, and this blower fan 7 is driven by a motor 12. A heating unit 13 is disposed in the blower fan 7 portion in the analysis chamber 6. That is, the air heated by the heating means 13 is blown by the blower fan 7 toward the great circle portion 6 b in the analysis chamber 6. In the great circle portion 6b, as described above, the analysis container 4 that is pivotally supported by the rotary drive shaft 10 and the bearing 9 and is rotated by the motor 11 is arranged. The rotation direction of the blower fan 7 and the rotation direction of the analysis container 4 rotated by the motor 11 are the same direction. Therefore, as shown in FIG. 15, the air blown by the blower fan 7 is blown from the small circle portion 6a of the analysis chamber 6 to the large circle portion 6b, and then from the large circle portion 6b portion to the small circle. The circulation path returning to the portion 6a is followed.

  Further, in this circulation path, particularly in the great circle portion 6b, four temperature sensors 14 are arranged at intervals of 90 degrees on the outer peripheral portion of the rotary drive shaft 10 as shown in FIG. The temperature of the great circle portion 6b in the analysis chamber 6 is detected.

  That is, as shown in FIG. 16, the temperature sensor 14 is connected to the control unit 15, and the heating unit 13 is connected to the control unit 15. Therefore, the heating unit 13 is controlled by the control unit 15, and as a result, The temperature of the analysis chamber 6, particularly the large circle portion 6 b, is kept at a set value, whereby the reaction of the analysis container 4 is stably performed, and as a result, the accuracy of gene analysis is increased. It is something that can be done.

  This point will be described in more detail. In the present embodiment, as described above, the blower fan 7 and the heating means 13 are provided in the small circle portion 6a of the analysis chamber 6, and the great circle portion 6b of the analysis chamber 6 is further provided. In this state, the analysis container 4 is rotated by the motor 11. For this reason, since the temperature in the analysis chamber 6 is constant as shown by line A in FIG. 17, the reaction of the analysis container 4 is performed stably, and as a result, the accuracy of gene analysis can be increased. It can be done. In particular, in this embodiment, the rotation direction of the blower fan 7 and the rotation direction of the analysis container 4 rotated by the motor 11 are the same direction. Therefore, as shown in FIG. 15, the air blown by the blower fan 7 is blown from the small circle portion 6a of the analysis chamber 6 to the large circle portion 6b, and then from the large circle portion 6b portion to the small circle. Since the circulation path returning to the portion 6a is followed, there is little stagnation of air. Also from this point, the temperature in the analysis chamber 6 is constant as shown by line A in FIG. As a result, the analysis accuracy of the gene can be increased.

  Furthermore, since the rotation direction of the blower fan 7 and the rotation direction of the analysis container 4 rotated by the motor 11 are the same direction, a collision of blown air occurs between the small circle portion 6a and the large circle portion 6b of the analysis chamber 6. As a result, the generation of noise due to turbulence in the air can be suppressed.

  Note that the Y axis in FIG. 17 indicates the temperature of the analysis container 4 detected by the temperature sensor 14, particularly the temperature of the great circle portion 6 b, but when the rotation speed of the analysis container 4 is low (starting up), the temperature However, it is understood that when the rotation speed of the analysis container 4 reaches the set value, the temperature of the great circle portion 6b of the analysis chamber 6 is stabilized.

  Further, the display unit 16 in FIG. 16 is disposed above the opening 3 as shown in FIG. 1 and is used for displaying, for example, a driving state and a detection result.

  Further, as described above, the measurement unit 17 is for reading a gene from below the rotationally driven analysis container 4 with the optical sensor 18 shown in FIGS. 10 to 13, and the measurement unit 17 and the display unit 16. Since these are known from the past, detailed description thereof is omitted to avoid complication of the description.

  Further, reference numeral 19 in FIG. 16 denotes a power button 19 provided below the opening 3 as shown in FIG.

  As described above, this embodiment includes the analysis chamber 6, the blower fan 7 provided in the analysis chamber 6, the heating means 13 for heating the air blown by the blower fan 7, and the analysis chamber 6. The analysis container rotation driving means 8 is arranged in the air blowing direction of the blower fan 7 so as to be separated by a predetermined distance, and the rotation direction of the blower fan 7 and the analysis container rotation drive means 8 is the same direction. Can be increased.

  That is, in the present embodiment, the air heated by the heating unit 13 is blown by the blower fan 7 toward the analysis container rotation drive unit 8 and the analysis container 4 rotated by the analysis container rotation drive unit 8. However, since the rotation direction of the analysis container rotation driving means 8 is the same as that of the blower fan 7, air is stirred in the analysis container rotation driving means 8 and the analysis container 4 portion. As a result, the temperature unevenness in the analysis container rotation driving means 8 and the analysis container 4 is extremely reduced, and as a result, the analysis accuracy can be improved.

  Further, since the analysis container rotation drive means 8 and the analysis container 4 have the same rotation direction as that of the blower fan 7, the analysis fan rotation drive means 8 and the analysis container 4 portion No air collision occurs between them, and as a result, the generation of noise can be suppressed.

  When the analysis of the gene using the analysis container 4 is completed, the analysis container carry-in tray 2 is pulled out of the main body case 1 from the opening 3 as shown in FIGS. The analysis container rotation driving means 8 gradually descends in response to the withdrawal of the analysis container. In the state shown in FIG. 11, the analysis container 4 is placed on the analysis container carry-in tray 2. Can be smoothly drawn out from the analysis container insertion opening 6 c of the analysis chamber 6 and the opening 3 of the main body case 1.

  With the above, the basic configuration of the embodiment of the present invention has been described, and more specific description will be given for each component.

  FIG. 18 shows a top view of the analysis container 4.

  The analysis container 4 is, for example, for analyzing a gene of a specimen. As an external configuration, the analysis container 4 has a main body case 21 and a rotation shaft into which a rotation shaft provided at the center of the main body case 21 is inserted. The insertion hole 5a, the opening 5 for injecting the specimen, the lid 20 for sealing the opening 5, and the rotation shaft center in the rotation shaft insertion hole 5a are provided on the circumference thereof. A first air suction port 22 and a second air suction port 23 provided on the radius with respect to the rotation axis center are provided.

  A cross-sectional view taken along line A in FIG. 18 is shown in FIG. An air discharge port 24 is provided on the side surface of the analysis container 4, and is connected by a first air suction port 22, a second air suction port 23, and a flow path 25 in the main body case 21 of the analysis container 4. ing.

  With such a configuration, when the analysis container 4 is mounted on the analyzer and driven to rotate, it flows from the first air suction port 22 and the second air suction port 23 as shown in FIG. The discharged air passes through the flow path 25 and escapes from the air discharge port 24.

  A reaction part 26 shown in FIG. 21 is provided in the main body case 21 along the flow path 25.

  The first air suction port 22 and the second air suction port 23 are provided on the inner peripheral side of the reaction unit 26, and the air discharge port 24 is provided on the outer peripheral side of the reaction unit 26.

  With the above configuration, when the main body case 21 is rotated by the rotary drive shaft 10, the heated air is sucked in by the negative pressure generated in the rotary drive shaft 10, and then the heated air is sucked into the first air. The air is sucked into the main body case 21 from the mouth 22 and the second air suction port 23, and then passes through the outer periphery of the reaction unit 26, and is then discharged from the air discharge port 24 to the outside of the main body case 21. . That is, in the present invention, the temperature-controlled heated air passes directly through the outer periphery of the reaction unit 26, so that the temperature of the reaction unit 26 can be maintained at the set temperature, and as a result, the analysis accuracy is improved. It can be increased.

  Moreover, the opening area of the air outlet 24 is configured to be larger than the opening areas of the first air inlet 22 and the second air inlet 23. As a result, the efficiency of sucking and discharging air is further improved, so that the reaction section 26 can be kept at a set temperature.

  Next, the reaction unit 26 will be described in detail.

  FIG. 21 shows a top perspective view of the analysis container 4.

  In the body case 21 of the analysis container 4, a reaction unit 26 is provided on the circumference of the center of the rotation axis. The reaction unit 26 includes a quantification unit 27 for quantifying the sample, a plurality of measurement chambers 28 provided on the outer periphery of the quantification unit 27 and containing a reagent that reacts with the sample, and the quantification unit 27 and a plurality of measurements. A flow path 29 that connects the chambers 28, and a sealing material 29a that seals the flow path 29 below a predetermined temperature and opens the flow path 29 when a predetermined time elapses when the predetermined temperature is exceeded. A stirring chamber 30 provided on the outer periphery of the plurality of measurement chambers 28 and provided for stirring the specimen and the reagent entering each measurement chamber 28, and a flow path 31 for connecting the measurement chamber 28 and the stirring chamber 30 respectively. It is comprised by.

  As shown in FIG. 21, the shape of the main body case 21 viewed from the upper surface, that is, the outline line of the cross-sectional shape, includes the first regions 32a and 32b having a relatively long distance from the rotation axis center and the rotation axis. The second regions 33a and 33b have a relatively short distance from the center, and the outline of the cross-sectional shape has a shape that is point-symmetric with respect to the rotation axis center.

  With the above configuration, the main body case 21 is rotated by the rotary drive shaft 10, so that the main body case 21 is blown onto the surface of the main body case of the analysis container that directly rotates the hot air from the blower fan. Is non-disk-shaped, and in the second regions 33a and 33b, the distance from the rotation axis center is relatively short, air circulation occurs on the upper side and the lower side of the analysis container 4, and the air is stirred. As a result, the temperature unevenness on the upper and lower surfaces of the analysis container 4 becomes extremely small, and the reaction part 26 of the analysis container 4 reaches the target temperature, so that the analysis accuracy can be improved as a conclusion. is there.

  And the reaction part 26 becomes the structure provided on the circumference | surroundings of 1st area | region 32a, 32b. Furthermore, the reaction part 26 is provided at a position longer than the radial length of the second regions 33a and 33b.

  Thus, by providing the reaction part 26 in the first regions 32a and 32b having a relatively long distance from the rotation axis center, the distance from the rotation axis center of the reaction part 26 is provided at a relatively long position. Therefore, since the centrifugal force applied to the reaction unit 26 can be further increased, the reaction of the reaction unit 26 is increased, and as a result, the efficiency of analysis can be increased.

  Returning to FIG. 21 again, the flow path confirmation means 34 will be described. The flow path confirmation unit 34 includes a first storage unit 35 and a second storage unit 36 arranged at predetermined intervals in the outer peripheral direction of the rotation shaft hole, and the first and second storage units 35 and 35. , 36, a wax 38 sealing the wax channel, and an opening detection material 39 accommodated in the first accommodating portion 35, and the first accommodating portion 35. When the opening detection material 39 stored in the container moves to the second storage unit 36 due to the melting of the wax 38, the specimen stored in the quantitative unit 27 moves to the measurement chamber 28 due to the melting of the sealing material 29a. It was configured to be slower than the time to do.

  As one of the more specific configurations, the melting temperature of the wax 38 in the wax flow path 37 connecting the first and second storage units 35 and 36 is higher than the melting temperature of the sealing material 29a. It was.

  As a second configuration, the cross-sectional area of the wax flow path 37 connecting the first and second storage units 35 and 36 is the cross-sectional area of the flow path connecting the fixed quantity unit 27 and the plurality of measurement chambers 28 respectively. It was configured to be wider. With this configuration, the amount of the wax 38 in the wax channel 37 is larger than the amount of the sealing material 29a in the channel 29 that connects the measurement chamber 28 and the quantification unit 27, so that the melting takes time. As a result, the time for the unsealing detection material 39 stored in the first storage unit 35 to move to the second storage unit 36 due to the melting of the wax 38 is the time when the sample stored in the quantification unit 27 is sealed. The time for moving to the measurement chamber 28 is delayed by the melting of the stopper 29a.

  With such a configuration, it is possible to determine whether or not the sample in the measurement chamber can be analyzed by checking whether or not the unsealing detection material 39 has flowed into the second storage unit 36. Therefore, the second storage unit By confirming the opening detection material 39 at 36, no extra measurement waiting time is provided, and as a result, the analysis time can be increased.

  Next, the rotating shaft insertion hole 5a will be described.

  FIG. 22A shows a top view of the analysis container 4, and FIG. 22B shows an enlarged view of the portion A, showing the rotation shaft insertion hole 5a.

  The outer contour line of the cross-sectional shape of the rotation shaft insertion hole 5a is recessed at the inner peripheral direction between the plurality of convex portions 40 arranged at predetermined intervals and projecting toward the outer peripheral direction and between the adjacent convex portions 40. It is set as the structure which has the concave part 41 and the continuous curve part 42 which connects these convex part 40 and the recessed part 41. As shown in FIG.

  Next, the configuration of the rotary drive shaft 10 is shown in FIGS.

  FIG. 23 is a perspective view of the rotary drive shaft 10, FIG. 24 (a) is a top view of the rotary drive shaft 10, (b) is a side view, and (c) is a longitudinal sectional view.

  As shown in FIG. 23, the rotary drive shaft 10 has a three-tiered structure including an insertion portion 43, a contact portion 44, and a bottom portion 45 from above.

  As shown in FIG. 24A, the outline of the cross-sectional shape of the insertion portion 43 is arranged at predetermined intervals and protrudes toward the outer peripheral direction, and adjacent convex portions 46. A concave portion 47 that is recessed in the inner circumferential direction in between, and a continuous curved portion 48 that connects the convex portion 46 and the concave portion 47 are used.

  FIGS. 25A and 25B show the analysis container 4 mounted on the analysis device.

  FIG. 25 (a) shows that the rotational drive shaft 10 is not inserted into the rotational shaft insertion hole 5a, and in this embodiment, the analysis container 4 is moved upward from below, thereby rotating the rotational shaft insertion hole. 5a is inserted into the rotary drive shaft 10.

  At the time of insertion, the rotational drive shaft 10 is in a neutral state in which it freely rotates. The curved portion 42 of the outer cross-sectional shape of the rotational shaft insertion hole 5a and the curved portion of the transverse sectional shape on the rotational drive shaft 10 side. The concave portions and the convex portions are engaged with each other while 48 is in contact with each other and sliding along the curved surfaces of the curved portions 42 and 48 of each other. More specifically, the rotational drive shaft 10 that is in a neutral state in the rotational direction is inserted into the rotational shaft insertion hole 5a of the analysis container 4 while performing a slight rotation.

  With such a configuration, the curved portion 42 of the outer cross-sectional shape of the rotary shaft insertion hole 5a and the curved portion 48 of the cross-sectional shape on the rotary drive shaft 10 side are in contact with each other and each other. The concave portion and the convex portion engage with each other while sliding along the curved surfaces of the curved portions 42 and 48, and the rotational drive shaft 10 can be inserted into the rotational shaft insertion hole 5a. Therefore, the operability when mounting the analysis container 4 is improved. It can be increased.

  Furthermore, in the analysis container 4 according to the embodiment of the present invention, the rotating operation at the time of analysis is a sudden rotation and a sudden brake operation in order to stir the specimen. Since 10 and the rotary shaft insertion hole 5a are firmly engaged by the convex part and the concave part, it does not slip in the rotational direction, so that the reliability of the analysis can be improved.

  Next, insertion of the analysis container 4 into the analysis device will be described with reference to FIG.

  In FIG. 26 (a), an analysis container insertion opening 6c is provided in the great circle portion 6b of the analysis chamber 6, and the analysis container carry-in tray 2 can be moved into and out of the analysis chamber 6 from the analysis container insertion opening 6c. .

  In addition, in the great circle portion 6b of the analysis chamber 6, an analysis container rotation drive means 8 is provided. The analysis container rotation drive means 8 includes a bearing 9 that rotatably supports the analysis container 4, As shown in FIGS. 26 (a) to 26 (d), the bearing 9 is lifted upward when the analysis container carry-in tray 2 is inserted into the analysis chamber 6 from the analysis container insertion opening 6c. The analysis container 4 is coupled to the rotational drive shaft 10 provided above the analysis chamber 6 by pivotally supporting the rotation shaft insertion hole 5 a of the analysis container 4. Further, since the rotation drive shaft 10 is connected to the motor 11, in the state of FIG. 26D, the analysis container 4 is pivotally supported while being sandwiched between the rotation drive shaft 10 and the bearing 9. The motor 11 can be rotated.

  In the present embodiment, a bearing block provided below the analysis chamber 6 as a bottom surface of the analysis chamber 6 provided with the bearing 9 and an analysis container carry-in tray 2 provided above the main body case. And a drive block as an upper surface of the analysis chamber 6 provided with a rotary drive shaft 10 and a motor 11. The bearing block and the drive block are in a state where the analysis container carry-in tray 2 is housed in the main body case. Since it is set as the structure moved toward the analysis container carrying-in tray 2 side, analysis accuracy can be improved.

  That is, in the embodiment of the present invention, the analysis chamber 6 in the analysis apparatus in which the analysis container carry-in tray 2 is placed is sandwiched between the bearing block and the drive block, and becomes a closed space surrounding the analysis container carry-in tray 2. Since the air in the chamber 6 is blocked from the air outside the analysis chamber 6, the temperature in the analysis chamber 6 can be easily maintained at the target temperature, and as a result, the analysis accuracy can be improved.

  In this embodiment, the bearing block is provided below the analysis chamber 6 and the drive block is provided above the analysis chamber 6. However, the bearing block is provided above the analysis chamber 6 and the drive block is provided below the analysis chamber 6. It is good also as a structure provided in the side.

  Next, the analysis process in this embodiment will be described with reference to FIG.

  FIG. 27A shows the temperature state in each process in the analysis chamber 6. FIG. 27B shows the rotational speed of each process of the analysis container 4.

  First, in the preparation step, as shown in FIG. 27 (c), the sample solution is injected from the opening 5 of the analysis container 4. The analysis container 4 is placed on the analysis container carry-in tray 2 as described above and inserted into the analysis apparatus.

  Next, regarding the determination step, as shown in FIG. 27 (d), the analysis container 4 inserted into the analyzer is accelerated to 3500 rpm and rotated for 10 seconds. In this state, the sample solution that has entered from the opening 5 of the analysis container 4 is distributed evenly to the quantification unit 27 by centrifugal force.

  Next, regarding the WAX dissolution step, first, as shown in FIG. 27A, after the determination step, the inside of the analysis chamber 6 is controlled to be heated to the target temperature of 60 ° C. by the heating means 13, and As shown in 27 (b), the rotational speed of the analysis container 4 is lowered to 100 rpm and held.

  In this state, as shown in FIGS. 27 (d) to 27 (e), the sealing material 29a in the flow path 29 that connects the measurement chamber 28 and the quantitative unit 27 in the analysis container 4 has a temperature environment of 60 ° C. In this case, the specimen is melted and the specimen stored in the quantification unit 27 moves to the measurement chamber 28 by melting of the sealing material 29a.

  As described above, whether or not the sample stored in the quantification unit 27 has moved to the measurement chamber 28 is determined by the first detection unit 35 shown in FIG. By confirming that it has moved to the second storage part 36, it can be performed reliably.

  Next, as shown in FIG. 27 (b), as shown in FIG. 27 (b), as shown in FIG. 27 (b), the number of rotations of the analysis container 4 is 3000 rpm for 5 seconds and 100 rpm for 5 seconds. The sample solution and the reagent in the measurement chamber 28 are stirred while going back and forth through the flow path 31 that connects the measurement chamber 28 and the stirring chamber 30 respectively.

  Next, with respect to the measurement process, as shown in FIG. 27A, the analysis container 4 is kept in a temperature environment of 60 ° C. as shown in FIG. Is maintained at 240 rpm. This state is maintained for 60 minutes, and the measurement is performed by observing the fluorescence in each measurement chamber as shown in FIG.

  FIG. 28 shows a flowchart of the measurement process.

  As a basic flow, as described above, the number of rotations of the analysis container 4 is maintained at 240 rpm, and after 60 minutes have passed in this state, as a final amplification determination process (S4), the intensity of fluorescence in each measurement chamber is increased. The presence or absence of a specific gene is determined depending on whether or not the absolute value is greater than a predetermined value. Then, the final result is displayed and output on the display unit 16.

  In such a discriminating method, it is necessary to observe the intensity of fluorescence after a lapse of a predetermined time, so that the analysis time is long. A method for solving such a problem will be described below.

  First, a gene amplification means is used for a specimen, and the specimen is reacted with a reagent containing a substance that binds a fluorescent reagent to a primer that reacts specifically with a gene of a specific sequence, and the presence or absence of the specific gene is detected. A method of analyzing a gene, wherein after the start of amplification of a specific gene for a specimen, the presence or absence of the specific gene is differentiated from the fluorescence intensity of the fluorescent reagent that specifically reacts to the specific gene. The analysis time can be shortened by the determination method when the value is larger than the predetermined value.

  A more specific description will be given with reference to FIG.

  In the measurement step, after a predetermined time has elapsed, if positive, that is, if a specific gene is present, the amount of change of amplification of that gene falls within a specific range. The amount of change is observed sequentially in the measurement process. Specifically, the differential value of the intensity of fluorescence is measured. By observing the values of the first and second derivatives of this differential value, the amount of change that the gene amplifies becomes a specific value. It is confirmed whether it is within the range (S1, S2 in FIG. 28).

  If this amount of change is within a specific range, it is determined to be positive and displayed on the display unit 16 (S3 in FIG. 28).

  That is, in the present embodiment, the amount of change in fluorescence intensity of the fluorescent reagent that reacts specifically with a specific gene is sequentially observed as a differential value, and the presence or absence of the gene is determined based on this differential value. The presence / absence of the change can be immediately determined based on the amount of change, and the analysis time can be shortened.

  Furthermore, this change amount is determined by the fact that the fluorescence intensity, that is, when the amount of the gene exceeds a predetermined threshold, the differential value of the fluorescence intensity is greater than a specific value. By using the determination step, more accurate determination can be performed.

  As described above, the present invention provides a main body case, a rotary shaft insertion hole that is provided at the center of the main body case and into which the rotation shaft is inserted, and a circumference within the main body case with the rotation shaft insertion hole as a center. The analysis part can be improved because the reaction part provided on the inner side of the reaction part, the suction port provided on the inner peripheral side of the reaction part, and the outlet provided on the outer peripheral side of the reaction part are provided.

  That is, in the present invention, since the suction port is provided inside the reaction portion of the main body case and the discharge port is provided outside the reaction portion, the main case is rotated by the rotation shaft, so that the negative shaft generated in the rotation shaft portion. The heated air is sucked in by pressure, and then this heated air is sucked into the main body case from the suction port, then passes through the outer periphery of the reaction part, and is then discharged out of the main body case from the discharge port. . That is, in the present invention, the temperature-controlled heated air passes directly through the outer periphery of the reaction part, so that the temperature of the reaction part can be maintained at the set temperature, and as a result, the analysis accuracy is improved. Can do it.

  Therefore, application as an analysis apparatus for genes and the like is highly expected.

DESCRIPTION OF SYMBOLS 1 Main body case 2 Analysis container carrying-in tray 3 Opening part 4 Analysis container 5 Opening part 5a Rotation shaft insertion hole 6 Analysis chamber 6c Analysis container insertion opening part 7 Blower fan 8 Analysis container rotation drive means 9 Bearing 10 Rotation drive shaft 11 Motor 12 Motor DESCRIPTION OF SYMBOLS 13 Heating means 14 Temperature sensor 15 Control part 16 Display part 17 Measurement part 18 Optical sensor 19 Power button 20 Lid 21 Main body case 22 1st air inlet 23 Second air inlet 24 Air outlet 25 Flow path 26 Reaction part 27 Quantitation Unit 28 Measurement Chamber 29 Channel 29a Sealing Material 30 Stirring Chamber 31 Channel 32a, 32b First Region 33a, 33b Second Region 34 Channel Confirmation Means 35 First Storage Unit 36 Second Storage Unit 37 Wax flow path 38 Wax 39 Opening detection material 40 Convex part 41 Concave part 42 Curve Part 43 insertion portion 44 contact portion 45 bottom portion 46 protrusion 47 recess 48 curved portion

Claims (2)

A body case,
A rotation shaft insertion hole provided in the center of the main body case, into which the rotation shaft is inserted;
A reaction portion provided on a circumference in the main body case around the rotation shaft insertion hole;
A suction port provided on the inner peripheral side of the reaction section;
An analysis container comprising a discharge port provided on the outer peripheral side of the reaction unit. The analysis container according to claim 1, wherein an opening area of the discharge port is configured to be larger than an opening area of the suction port.

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