JP2014010076A - Measurement device for advanced glycation end product - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an AGEs(Advanced Glycation End products) measurement device which is compact, inexpensive, excellent in operability, and simple by directly converting fluorescent emission resulting from the AGEs from the skin of a person by the excitation rays of light from an LED light emission section into an electric signal by a light reception section without using any conventional optical or indirect optical transmission means.SOLUTION: A measurement head 1 of a measurement device is configured in a small and compact shape so as to be gripped with the hand of a person, and an LED light emission section 12 which concentrically emits irradiation rays of light as a light emission section and a base integration type spectral photometer element 15 as a light reception section 14 are consolidated and internally mounted on the measurement head 1. Fluorescent emission resulting from AGEs from the skin is directly converted into an electric signal by the spectral photometer element of the light reception section 14 so as to be detected.

Description

  The present invention makes use of the fluorescent properties of advanced glycation end products (AGEs) that are produced and accumulated in the body with age, and thus various internal diseases such as diabetes and diabetic retinal detachment, kidneys, and the like. Simplified measurement for non-invasive detection and measurement of AGEs to determine the degree of aging and adult disease such as disease, arteriosclerosis, Alzheimer's disease, osteoporosis, cancer and skin sclerosis With regard to the device, in order to enable measurement at the optimal part of the human body that is not easily affected by pigments of the skin tissue such as race and sunburn, the light emitting part and the light receiving part are integrated into the measuring head that is pressed against the skin and is compact. The present invention relates to an apparatus for measuring a saccharified final product that is compact and can be detected and measured by simply pressing it to an optimal site.

  In general, various glycation end products (AGEs) are accumulated in the human body with aging, and the accumulated amount of AGEs in the blood and human tissues, particularly in diabetic patients with hyperglycemia, which is the main cause of adult diseases. However, it is known that there are many AGEs compared to healthy people of the same age, and there are specific AGEs corresponding to specific diseases, and these AGEs are in the region of blue light (380 nm to 500 nm) or less. It is known that there are various saccharified substances such as those that emit a specific wavelength longer than the excitation light or those that do not emit fluorescence when irradiated with specific excitation light due to fluorescence characteristics derived from AGEs.

As a device for measuring or detecting specific AGEs, a mass spectrometer (MS) or high performance liquid chromatography (HPLC) that collects blood and cells from a human body and analyzes them is used. In recent years, attention has been paid to the development of so-called non-invasive measuring devices that do not impose the burden of collecting samples such as blood collection and cytology from humans. For example, specific excitation light is applied to skin tissue. Various detection and measurement devices have also been proposed, such as a large measurement device that irradiates and optically guides and detects the fluorescence emission of AGEs to the main body of the measurement device. Furthermore, it is also known that the possibility of various adult diseases and the like are diagnosed and diagnosed based on the measurement results of the amount of AGEs.

In particular, in the latter non-invasive apparatus for measuring AGEs, it should be noted that the color of the skin is race, sex, aging, sunburn, skin disease, skin tissue thickness, melanin content, Regarding blood hemoglobin level, etc., there is a great racial difference. For this reason, the measurement values obtained by the skin AGEs measurement device will be affected by the skin color, so it is necessary to correct the measurement data appropriately. There is. Furthermore, the influence and measurement error of reflected light obtained from the light irradiated on the skin and the originally necessary fluorescence derived from the AGE in the skin, or fluorescence generated from substances other than the AGE in the skin are minimized as much as possible. Various ideas for avoiding these problems have been made, and proposals for these general solutions are also known.

For example, as this kind of non-invasive AGEs detection and measurement device commercially available for research, a light source, a fluorescence spectrometer, and a data analysis device are built in a box-shaped casing having a detection window opened on the upper surface. An installation-type measuring device (trade name: AGE Reader) has been put into practical use. A person's arm is placed on the casing and irradiated with excitation light. The fluorescence from the arm is detected, and the disease state based on the measured value is detected. What is used for diagnosis and the like has been put into practical use, and the characteristic configuration of the patent document for which an international patent application has been filed with respect to the measurement device has been described below, using a black light fluorescent tube 2 provided in the case 6 as a light source and passing through a filter 5. Then, the skin tissue 7 is irradiated with excitation light having a specific wavelength, and the fluorescence emitted from the skin tissue 7 is optically guided to the detector 22 through the measurement window 18 through the fiber 3 and measured. Clinically and to grasp the autofluorescence properties in normal skin tissue of a patient, there is a method and apparatus for determining the autofluorescence properties of the skin tissue (Patent Document 1).

Furthermore, as a prior art relating to a conventionally known non-invasive AGE detection / measurement method of this kind and a diagnosis method of a disease state based on the detection / measurement value, for example, a light source subsystem in a part of human tissue Fluorescence derived from chemical substances (AGEs) in human tissue that responded to the excitation light by irradiating the excitation light from (A) and light emitted from the human tissue are detected as they are in the state of the image (C In order to determine the tissue state (AGE level or disease state), the detected light is transmitted to the signal processing device (D) to be related to a measure (determination value) of the tissue state. Related to determination of glycation end product or disease state scale using fluorescence emission of tissue, which is used in combination with a stored detection light model to make a disease diagnosis determination (Patent Document 2), or multiple light A method for detecting the biological material by causing a predetermined reaction to proceed to the biological material using excitation and optically deriving fluorescence from the autofluorescent material that is a reaction product of the reaction to the detection unit In addition, there is known a biological substance detection method using a laser (Patent Document 3) in which a disease state and physiological function are determined based on the detected fluorescence detection value.

Alternatively, as this type of non-invasive AGE detection / measurement apparatus, in order to eliminate variations in measurement values due to displacement of the irradiation position of excitation light by the conventional pinpoint (point) measurement method, ) Is equipped with a scanning mechanism 1 having an excitation light source 4 and a detector 8 for optically receiving fluorescence derived from the excitation light in a housing 3 having a window 3a on the upper surface, thereby measuring a measurement target portion. On the other hand, the excitation light source 4 and the light detector 8 can be moved in a plane in the housing 3 to measure (scan) the fluorescence derived from AGEs. With this configuration, multiple points can be obtained by a single scan. A large-scale measuring device system (Patent Document 4) that obtains measurement, that is, a measurement value as a surface, and eliminates variations in measurement results based on this abundant measurement information, and a mercury xenon lamp 18 A light source unit 12 having a first excitation light source and a halogen lamp 20 as a second excitation light source is provided, and the first and second inspection lights L1 and L2 from the light source unit 12 are supplied to the skin or the like. Reflected light such as fluorescence derived from AGEs from a first light guide part 32 such as glass fiber that is optically conducted to a predetermined part (examination part) W and a predetermined part (examination part) W such as skin. A handy type for irradiating the inspection part W as light and receiving it as light by the same second light guiding part 34 optically conducting to the separate measuring part 16 having the photodiode array 46 There is known a method for measuring fluorescence of skin and an apparatus therefor (Patent Document 5) provided with the irradiation unit 30.

WO01 / 022869 JP 2007-222669 A JP 2009-047540 A JP 2012-058104 A JP 2004-290234 A

However, since the black light fluorescent tube 2 used as the light source is long in the case of the apparatus as described in Patent Document 1, the case 6 for accommodating the light source is large even though the measurement site may be in a small range. The body part suitable for the object to be measured is limited to the skin tissue 7 on the person's arm or the like having a shape similar to that of the black light fluorescent tube 2. For this purpose, the case 1 is installed. It is necessary to put an arm on the measurement window 18 and there are restrictions on the selection of the optimal measurement site such as the inner side of the upper arm and the abdomen which are less affected by sunburn, etc. Since the black light fluorescent tube 2 is used as a light source, power consumption and heat generation energy are large, which is undesirable for sensitive skin tissues.
Further, in order to avoid the influence of irradiation light from the black light fluorescent tube 2 having a wide wavelength range, the light is condensed by an expensive glass fiber 3 and optically guided to the spectrophotometer 15 and the detector 22 in the light state. Therefore, the apparatus is inconvenient, such as an increase in size and cost.

  11 and 12 in Patent Document 2 includes a light source subsystem (A) that performs complicated filtering using a xenon arc lamp, a laser beam, or the like as an excitation light source, and a fluorescence detection device. A configuration in which a detection subsystem (C) obtained by performing complicated filtering on a photomultiplier tube (PMT) is guided to an individual tissue of a sample via a bifurcated fiber bundle as an optical delivery subsystem (B) Therefore, the fiber bundle used to optically send the detection light as it is to the detection subsystem (C) is expensive as in the above-mentioned Patent Document 1, and the signal processing device (D) has a hue. Because it requires a detection light model for discriminating color, it is accompanied by color discrimination errors, and the device is complicated and large, and cannot be handled portablely. , Were those constraints that are many disadvantages in use.

And since the detection apparatus used for the biological material detection method disclosed in FIGS. 5 and 6 of Patent Document 3 uses a laser light source 11 as a light source for multiphoton excitation, Instead of being able to measure and inspect AGEs, the power consumption and generated energy of the laser light source 11 are large and expensive, and the transmission path between the light source 11 and the specimen 4 includes an objective lens 28a for focusing and complicated filtering. In addition to the need for optical means, as shown in the apparatus of FIG. 6 in particular, an efficient optical fiber for optically transmitting detection light as in the above-mentioned Patent Documents 1 and 2. Because it uses 28c, it is expensive, and can be easily moved and used when viewed as a system that includes a laser light source. There was no.

Furthermore, the non-invasive measuring device and measuring system for skin AGE of Patent Document 4 and the measuring method are particularly shown in FIGS. 2, 3 and 4 and also described in the specification of the publication. An excitation light source 4 is composed of an ultraviolet light LED 4a (wavelength 230 nm to 365 nm) and a visible light LED 4b (wavelength 405 nm) arranged in a line in the housing 3 of the installation type detection device 10, and emits light from the light source 4. The excitation light thus emitted is directly applied to the measurement target part (skin tissue) from the window 3a, but the fluorescence and reflected light from the skin tissue is transmitted from the window 3a to the light receiving part side mounted in the housing 3 side. While being guided to the detector 8 through optical paths such as the fluorescence introduction slit 5, the light guide portion 6, and the lens 7, the light source 4 and the detection portion 8 are integrated as a scanning mechanism 1 by the drive mechanism 2. It is configured to move and scan freely Therefore, even if an electrical signal can be sent to the external measuring device 9, the optical paths 5, 6 and 7 to the detector 8 require a long inversion path, and the detecting device 10 itself is automatically Since the scanning mechanism 1 is an indispensable condition, the movable part involved in this scanning and driving must be complicated and large in size. It was not easy to carry, and was unsuitable for measurement at the optimal site of skin tissue, making it unusable.

Furthermore, in the method and apparatus for measuring skin fluorescence of Patent Document 5, the mercury xenon lamp 18 constituting the light source unit 12 is used as described in FIG. And the halogen lamp 20 as a second excitation light source, and these first and second inspection lights L1 (wavelength 335 nm) and L2 (wavelength 365 nm) are applied to a predetermined part such as skin. (Test site) W is irradiated from the irradiation unit 30 through the first light guide unit 32 such as glass fiber which is an optical conducting means, and then fluorescence derived from AGEs from a predetermined site (test site) W Is reflected from the irradiation unit 30 to the separate measurement unit 16 having the photodiode array 46 by the second light guide unit 34 which is also an optical conducting means. ,leather Although the operability of the irradiation unit 30 with respect to the optimal site such as is improved, the light source unit 12 and the measurement unit 16 that are configured separately are connected using an expensive optical conducting means, Just as in the above-mentioned conventional Patent Documents 1 to 3, not only is it expensive and costly, but the mercury xenon lamp 18 and the halogen lamp 20 of the excitation light source 12 are large in power consumption and large in size. Inevitably, the device must be installed and used for use, so it cannot be said that the device is convenient to carry.
In the test measurement, the measurement site such as the skin is first irradiated with the first test light L1 to measure the reflected fluorescence, and then the second test light L2 having a significantly different wavelength is applied. Thus, the reflected light is measured, and the fluorescence correction is performed using the reflected light data (see paragraph 0049 of the publication), so that the processing and operation become complicated and cannot be used easily.

  Therefore, the present invention has been made to improve and solve the above various problems, and the object of the present invention is to freely press the measuring head of the measuring device against the skin of the optimal measuring site of the human body. The shape of the measuring head is small and compact so that it can be held by a human hand, and a mounting member with a substantially hollow conical shape is provided inside the measuring head, and the same circumference of the inner surface of the cone is provided. A plurality of LEDs as light sources are arranged in a ring shape on the top, and an LED light emitting unit is provided in which these LED lights are concentrated and directed to one point on the central axis, and a light receiving unit is provided at the central top of the mounting member. As a base-integrated spectrophotometer element, the light receiving part and the measurement hole opened in the center of the end face of the measuring head are arranged on the same axis, and excitation from the LED light emitting part Light is measuring hole In addition to intensively irradiating the skin, a one-pass optical path is formed in which the reflected light from the skin enters the light receiving part through the measurement hole, and thereby the fluorescence derived from glycated end product substances (AGEs). Light emission is directly converted to an electrical signal by the spectrophotometer element of the light receiving unit and detected, so that it is possible to intensively irradiate powerful excitation light etc. while being an LED light source excellent in energy saving. An object of the present invention is to provide an inexpensive and simple measurement apparatus for a final saccharification product that is excellent in operability and enables portability (portability) and has low power consumption.

  Another object of the present invention is to increase the measurement accuracy by paying attention to the fact that there is a correlation between specific excitation light for specific glycation end products (AGEs) and fluorescence characteristics derived from AGEs. An ultraviolet light LED having a wavelength slightly shorter than the excitation light for the specific AGEs, as a combination of two or more types of ultraviolet light LEDs that emit specific excitation light for the specific AGEs, as the LED light emitting unit in the measurement head of the measurement apparatus And two or more kinds of fluorescence derived from AGEs from the skin by these two or more kinds of excitation light LEDs. The emitted light is converted into a measurement value electric signal amplified and amplified by the spectrophotometer element of the light receiving unit so that the measurement value can be detected, and there is no variation in the measurement value. Excellent sex, yet in that it enables portability (portable flexibility), yet provides a measuring device with low power consumption inexpensive glycation end product materials.

Furthermore, the object of the present invention is to produce specific glycation end products (AGEs), particularly AGEs that emit fluorescence such as crosslin, pyropyridine, and pentodine, or produce 3 deoxyglucosone (3DG) as a saccharification reaction intermediate. Specific excitation light wavelength of 370 nm for AGEs that emit simultaneously with the generation process of AGEs that do not emit fluorescence, such as carboxymethyllysine (CML), and fluorescence wavelengths derived from the corresponding AGEs Focusing on the correlation with 440 nm, as an LED light emitting part in the measuring head of the measuring apparatus, an ultraviolet light LED having a wavelength of 365 nm slightly shorter than the specific excitation light wavelength and an ultraviolet light LED having a slightly longer wavelength of 375 nm Combining two kinds of ultraviolet LED, this is adopted as the excitation light source, It is possible to detect and detect two fluorescence emission derived from AGEs from the skin by two kinds of adjacent excitation light LEDs such as, etc., by converting into a measurement value electric signal that is polymerized and amplified by the spectrophotometer element of the light receiving unit. An object of the present invention is to provide an inexpensive measurement apparatus for a final saccharification product with excellent measurement accuracy and excellent operability with respect to a fluorescence wavelength derived from AGEs.

Further, an object of the present invention is to provide an LED light emitting unit in a measurement head of a measurement apparatus in order to prevent the fluorescence measurement value derived from AGEs of skin tissue from being influenced by individual differences in skin color such as sunburn and melanin pigment. As an example, in addition to an excitation light source formed by combining two or more types of ultraviolet light LEDs, a white light LED having a relatively long wavelength, for example, a wavelength of 465 nm as an example, It is possible to correct individual differences in skin color such as gender, age, sunburn, skin diseases, etc., and to measure only the fluorescent component derived from AGEs, which is accurate, simple and easy to operate, and inexpensive saccharification The object is to provide an apparatus for measuring the final product.

Another object of the present invention is to provide a plurality of excitation light LEDs provided as LED light emitting units in a measurement head of a measurement apparatus so that various wide AGEs can be inspected and measured. Two or more kinds of ultraviolet light LEDs that emit specific excitation light for AGEs of AGEs are provided, and fluorescence emission derived from AGEs having higher fluorescence characteristics by any excitation light LED can be selected, and this fluorescence emission is selectively used. In addition, it is possible to detect and directly convert the measured value electrical signal to the spectrophotometer element of the light-receiving unit, and to perform inspection and measurement for some specific AGEs, which is multifunctionally accurate, simple and excellent in operability. An object of the present invention is to provide an inexpensive measuring device for a saccharified final product.

In order to achieve the above object, the problem-solving means of the present invention is to form the measuring head of the measuring device into a small and compact shape so that it can be grasped by a human hand. An LED light-emitting unit provided with a mounting member, in which a plurality of LEDs as light sources are arranged in an annular shape on the same circumference of the inner surface of the cone, and these LED lights are concentrated toward one point on the central axis In addition, a base-integrated spectrophotometer element as a light receiving part is integrated and installed at the center top of the mounting member, and the light receiving part and the measurement hole opened in the center of the end face of the measuring head are on the same axis. A one-pass type light that is arranged on a line so that the excitation light from the LED light emitting part is intensively applied to the skin from the measurement hole and at the same time, the reflected light from the skin enters the light receiving part from the measurement hole Configure the route and Fluorescence emission derived from saccharification end product (AGEs) is detected by directly converting it into an electrical signal with the spectrophotometer element of the light receiving unit, and the electrical signal related to this fluorescence measurement value is controlled in a separate operation box. The cable is transmitted to the base, and the control base can control the LED light emitting part and the spectrophotometer element of the light receiving part, and an electric signal related to the fluorescence measurement value is output as an input signal to the determination processing control part It is configured so that it can be used.

  In order to achieve the above-described object, the problem solving means of the present invention is to form the measurement head of the measurement device into a small and compact shape so that it can be grasped by a human hand, and the measurement head includes an LED as a light source. A base-integrated spectrophotometer element as a light emitting part and a light receiving part is integrated and incorporated, and as the LED light emitting part, two or more kinds of ultraviolet LEDs that emit specific excitation light for specific AGEs are combined. An excitation light source comprising a combination of two types of ultraviolet LEDs, an ultraviolet light LED having a wavelength slightly shorter than the excitation light for specific AGEs and an ultraviolet light LED having a slightly longer wavelength, is used. Detects two or more types of fluorescent light emission derived from AGEs from the skin by two or more types of ultraviolet light LEDs by converting them into measurement value electrical signals that are polymerized and amplified by the spectrophotometer element of the light receiving unit. The electric signal is transmitted to a control board in a separate operation box and the spectrophotometer element of the LED light emitting unit and the light receiving unit can be controlled by the control board. An electrical signal related to the fluorescence measurement value can be output as an input signal to the determination processing control unit.

Furthermore, in order to achieve the above-described object, the problem solving means of the present invention is directed to specific glycation end-product substances (AGEs), particularly AGEs that emit fluorescence such as crosslin, pyropyridine, and pentodine, or 3 deoxyglucosone (3DG ), Which is a saccharification reaction intermediate, and a specific excitation light wavelength of 370 nm for AGEs that emit fluorescence simultaneously with the generation of AGEs that do not emit fluorescence, such as carboxymethyllysine (CML), Focusing on the correlation with the fluorescence wavelength of 440 nm derived from AGEs corresponding to the ultraviolet light LED with a wavelength of 365 nm slightly shorter than the specific excitation light wavelength and a slightly longer wavelength as the LED light emitting part in the measurement head of the measuring device Combined with 375nm UV LED, this is used as excitation light source The two fluorescent light emission derived from AGEs from the skin by these two kinds of adjacent ultraviolet light LEDs can be detected by converting into a measurement value electric signal that is polymerized and amplified by the spectrophotometer element of the light receiving unit. It is characterized by comprising.

  In order to achieve the above object, the problem-solving means of the present invention is to make the shape of the measuring head of the measuring device small and compact so that it can be grasped by a human hand, and the measuring head has LED light emission of the measuring device. In addition to the excitation light source that combines two or more types of ultraviolet light LEDs as the LED light emitting part, a comparison is made with the spectrophotometer elements integrated into the base as the light emitting part and the light receiving part. For example, a white light LED with a wavelength of 465 nm is used as an example. The white light corrects individual differences in skin color such as race, sex, age, sunburn, and skin disease. It is characterized in that it is configured so as to be a measured value of only a fluorescent component derived from the above.

  In order to achieve the above-described object, the problem-solving means of the present invention provides a plurality of excitation light LEDs provided as LED light emitting units in a measurement head of a measurement apparatus, a plurality of ultraviolet LEDs or a specific excitation for a specific AGE. Two or more kinds of ultraviolet LEDs that emit light are provided, and fluorescence emission derived from AGEs having higher fluorescence characteristics by any excitation light LED can be selected. It is characterized in that it can be detected by being converted into a measured value electric signal by a measuring element.

  According to the present invention, as described in claim 1, the shape of the measuring head of the measuring device is formed in a small and compact shape so that it can be grasped by a human hand, and a substantially hollow conical shape is attached inside the measuring head. An LED light emitting unit is provided in which a plurality of LEDs as light sources are arranged in a ring on the same circumference of the inner surface of the conical inner surface so that these LED lights are concentrated on one point on the central axis. At the center top of the mounting member, a spectrophotometer element integrated with a base as a light receiving part is integrated and housed, and the light receiving part and the measurement hole opened in the center of the end face of the measuring head are on the same axis. The one-pass optical path in which the excitation light from the LED light emitting unit is intensively applied to the skin from the measurement hole and the reflected light from the skin enters the light receiving unit through the measurement hole. Make up the final saccharification Fluorescence emission derived from chemical substances (AGEs) is detected by directly converting it into an electrical signal at the spectrophotometer element of the light receiving unit, and this electrical signal related to the fluorescence measurement value is used as a control base in a separate operation box. It is possible to transmit the cable and to control the spectrophotometer element of the LED light emitting unit and the light receiving unit by the control base, and to output an electric signal related to the fluorescence measurement value as an input signal to the determination processing control unit As a result, it is possible to intensively irradiate the skin with excitation light, etc. while being an LED light source with excellent energy savings, which can improve the measurement performance as a device and has excellent operability. The detector head is pressed to the most suitable place for measurement in the area, that is, the most suitable part that is not easily affected by the pigment of skin tissue such as race and sunburn. It can be measured, thereby exerting an excellent effect that it is possible to dramatically improve the detection accuracy and usability.

In addition, since the entire measurement device has a simple structure, it can be provided as a portable and convenient portable measurement device, and the light emitting unit as a light source of the measurement device includes: Since a light emitting LED of a specific wavelength is adopted, the light source power source can be an inexpensive general power source, and the power consumption is low, which is economical and can provide a measuring device with a large economic effect without running costs.
Furthermore, in the measuring head, the detection light including the received fluorescence can be directly converted and measured by a spectrophotometer element, and transmitted as a cable as an electric signal. Therefore, it is possible to construct the apparatus at a very low cost compared with the conventional apparatus, and the measurement apparatus is excellent in economic efficiency in terms of initial investment. Can be obtained.

According to the present invention, as described in claim 2, the shape of the measuring head of the measuring device is formed in a small and compact shape so that it can be grasped by a human hand, and a substantially hollow conical mounting is provided inside the measuring head. A member is provided, and a plurality of LEDs as light sources are arranged in an annular shape on the same circumference of the inner surface of the cone, and an LED light emitting unit is provided in which these LED lights are concentrated to one point on the center line. In addition, a base-integrated spectrophotometer element as a light receiving part is integrated and installed at the center top of the mounting member, and the light receiving part and the measurement hole opened in the center of the end face of the measuring head are on the same axis. The one-pass type in which the excitation light from the LED light emitting unit is intensively applied to the skin from the measurement hole and the reflected light from the skin enters the light receiving unit through the measurement hole. Constitutes the optical path and the LE As the light emitting part, two or more kinds of ultraviolet light LEDs emitting specific excitation light for specific AGEs are combined, or an ultraviolet light LED having a wavelength slightly shorter than the excitation light for specific AGES and ultraviolet light having a slightly longer wavelength Employing an excitation light source in combination with LEDs, these two or more types of ultraviolet light LEDs are used to polymerize and amplify two or more types of fluorescence emission derived from AGEs from the skin using the spectrophotometer element of the light receiving unit. The measured signal is converted into an electric signal so that it can be detected, and the electric signal is transmitted to a control board in a separate operation box, and the spectrophotometer of the LED light emitting unit and the light receiving unit is transmitted by the control board. The device can be controlled and an electric signal related to the fluorescence measurement value can be output as an input signal to the determination processing control unit. Therefore, coupled with the strong irradiation light due to the concentrated light collecting effect in the LED light emitting part, the reflected light from the skin due to this irradiation light can not only be received in a powerful way, but also this In two or more types of fluorescent light emission in the reflected light, the so-called polymerization amplification effect can be obtained as a measurement output value and its spectrophotometric distribution can be highlighted, so that necessary measurement values can be detected reliably and selectively. Exhibits excellent effects that can be done.
Therefore, the variation in measured values can be suppressed, the measurement accuracy can be improved, and it is easy to operate with high accuracy, excellent operability, and excellent portability (portability) and power consumption. Therefore, it is possible to obtain an inexpensive measuring device for the final saccharified product.

Further, the apparatus for measuring a final saccharification product according to the present invention, as defined in claim 3, is intended for specific saccharification final product (AGEs), particularly AGEs that emit fluorescence such as croslin, pyropyridine, pentodine, or 3 Specific excitation light wavelength for AGEs that generate deoxyglucosone (3DG) as a saccharification reaction intermediate, and for AGEs that generate fluorescence simultaneously with the generation of AGEs that do not emit fluorescence such as carboxymethyllysine (CML) Focusing on the correlation between 370 nm and the corresponding fluorescence wavelength 440 nm derived from AGEs, an ultraviolet light LED having a wavelength of 365 nm slightly shorter than the specific excitation light wavelength is used as the LED light-emitting part in the measuring head of the measuring apparatus. And a slightly longer ultraviolet LED with a wavelength of 375 nm, which is used as an excitation light source The two fluorescence emission derived from the AGEs from the skin by these two kinds of adjacent ultraviolet light LEDs are converted into a measurement value electric signal that is polymerized and amplified by the spectrophotometer element of the light receiving unit. Since it can be detected, it is possible to reliably detect and measure a specific AGE as a target, and to exhibit excellent effects such as low cost.

  Furthermore, the measurement apparatus for a saccharification final product according to the present invention is an excitation light source comprising a combination of two or more types of ultraviolet LEDs as an LED light emitting unit in a measurement head of a measurement apparatus. In addition, since a white LED with a relatively long wavelength is also provided, the color of the absorbed light in the skin tissue is corrected with this white light, and individual differences in skin color are corrected to measure fluorescence derived from AGE. The value can be detected and measured by highlighting only the fluorescent component, and the measurement focused on fluorescence emission can be performed accurately and reliably without being influenced by the environmental conditions of the skin tissue. Can be made inexpensively and easily, and its practical effect is great economically.

  Furthermore, the measurement apparatus for a saccharification final product according to the present invention is the plurality of excitation light LEDs provided as the LED light emitting section in the measurement head of the measurement apparatus, as described in claim 5. Two or more kinds of ultraviolet light LEDs that emit specific excitation light for AGEs of one of them are provided, and fluorescence emission derived from AGEs having higher fluorescence characteristics by any excitation light LED can be selected, and this fluorescence emission is selectively Since the spectrophotometer element of the light receiving unit is converted into a measurement value electric signal and can be detected, a wide variety of AGEs can be inspected and measured with high accuracy, and it is simple, excellent in operability, and inexpensive. Even an apparatus for measuring a final saccharification product exhibits an excellent effect that can be made versatile with multiple functions.

It is a perspective view for explanation showing the whole composition of the measuring device of the final saccharification substance in one embodiment of the present invention. It is a side view of the measurement head part which comprises the measuring apparatus in embodiment of FIG. 1 of this invention. It is explanatory drawing of the pressing surface which looked at the measurement head part shown in FIG. 2 in embodiment of this invention from the A direction. It is a longitudinal cross-sectional view for description of the measurement head part shown in FIG. 2 in embodiment of this invention. It is explanatory drawing which removed the contact cover which is a pressing surface of the measurement head part shown in FIG. 2 in embodiment of this invention, and was seen from A direction. It is an explanatory enlarged view which shows one Example of the attachment point of ultraviolet light LED as a light source in embodiment of this invention. It is an explanatory enlarged view showing an example of a light receiving unit that receives reflected light such as fluorescent light emission in the embodiment of the present invention. It is an explanatory enlarged view which shows one Example of the attachment point of white LED as a light source in embodiment of this invention. It is a graph which shows an example of the measurement detection result of two types of fluorescence emission derived from the specific AGE by the two types of excitation light close | similar to the specific excitation light wavelength as a light source in embodiment of this invention.

The present invention will be described below based on the embodiments shown in the drawings.
FIG. 1 is an explanatory perspective view showing the entire configuration of a measurement apparatus for a glycated end product substance in one embodiment, and the measurement apparatus of the present invention is freely pressed against the skin of an optimal measurement site of a human body. In addition, a measurement head 1 formed in a compact and compact shape that can be grasped by a human hand, and a substrate-integrated spectrophotometer element as an LED light-emitting unit 12 and a light-receiving unit 14 described later built in the measurement head 1 15 receives an operation box 2 mainly composed of a control board 2a that controls power supply control, detection light output control, and the like, and a measurement electric signal output of the detection light from the operation box 2, and a spectral distribution waveform of the detection light A personal computer 3 having a display screen 3a for displaying, and having control software for diagnosing the progress of disease or disease from the obtained measured value data, or for determining processing More composed.

The operation box 2 is equipped with a power supply connector 2b, a changeover switch 2c for the LED light emitting unit 12, and a changeover switch 2d for the spectrophotometer element 15 integrated with the base. The measurement head 1 and the operation box 2 are also provided. Is a bundle of power supply cables 4a and 4b for supplying power to the LED light emitting unit 12 and the spectrophotometer element 15, and a wiring cable 4c for transmitting an electrical signal of the fluorescence spectrophotometric distribution from the spectrophotometer element 15. The operation box 2 is connected to a personal computer 3 having a determination processing control function through a USB cable 5 which is connected by a cable 4 and transmits an output signal from the control board 2a.
In addition, about the determination method regarding the prediction and diagnosis of aging, an adult disease, etc., the processing software, etc. which are performed in the personal computer 3, description is abbreviate | omitted here.

Next, the configuration relating to the measuring head 1 of the measuring apparatus, which is the greatest feature of the present invention, will be described in detail below with reference to FIGS.
The measuring head 1 of the present invention is formed into a small and compact shape so that it can be grasped by a human hand, and is formed in a substantially cup shape in the case of illustration (however, the outer shape is limited to this cup shape). (Not a thing) It consists of a hollow measuring head main body 1a, and a disc-shaped contact is formed at the opening end on the large diameter side of the hollow measuring head main body 1a so as to form a contact surface for pressing against the skin. A cover 10 is attached, and a measurement hole 10a for allowing reflected light including excitation light to the skin and fluorescence from the skin to pass through is opened in the center of the contact cover 10 (see FIG. 3). A cable 4 connected to the control board 2a in the operation box 2 is connected to the end of the head main body 1a on the small diameter side.

In the case of the embodiment of the present invention, the abutting cover 10 is black as a whole and prevents irregular reflection of light from portions other than the measurement hole 10a. The extra light to be irradiated can be absorbed.
Further, since the contact cover 10 is a portion that directly presses against the human skin, a hole having a similar shape on the surface, that is, a hole corresponding to the measurement hole 10a is opened in the center (not shown). A disc-shaped sanitary film, or a felt-absorbing felt material that absorbs sweat, moisture-absorbing paper, etc. can also be attached, so that the subject can feel comfortable without feeling uncomfortable. In addition, hygienic AGE measurement and inspection is possible.

As shown in detail in FIG. 4, a mounting member 11 having a substantially hollow conical shape is fixed and supported in the measuring head main body 1 a, and on the same circumference of the inner conical surface of the mounting member 11. Is a plurality of excitation light sources for specific glycation end products (AGEs), for example, in the case of the illustrated embodiment, two kinds of ultraviolet LEDs 12a and 12b having different wavelengths close to a specific wavelength, White light LEDs 13 to be described later for color correction are alternately arranged in an annular shape to constitute an LED light emitting unit 12 with low power consumption, and these ultraviolet light LEDs 12a embedded in the inner peripheral surface of the cone. , 12b and the white light LED 13 are arranged so that the excitation light and the correction light are directed toward one point of the measurement hole 10a at the center of the contact cover 10 and are intensively irradiated.
Note that the ultraviolet LEDs 12a and 12b serving as excitation light sources that emit specific excitation light for specific AGEs are not limited to two types, and may have two or more specific wavelengths as long as the polymerization effect described below is obtained. An ultraviolet light LED can be used, or even a single kind of ultraviolet light LED that emits excitation light of a specific wavelength is used as a condensing arrangement structure in which a plurality of such LEDs are arranged in an annular shape. It is also possible to implement.

Further, the central portion corresponding to the top of the conical portion of the mounting member 11 maintains the positional relationship on the same axis so as to face the measurement hole 10a of the abutting cover 10, and the light receiving hole 14a of the light receiving portion 14 and the substrate integrated type A spectrophotometer element 15 is installed. Due to these arrangement relationships, the excitation light and the correction light from the LED light emitting unit 12 are concentrated and irradiated from the skin through the measurement hole 10a. It is configured to form a one-pass optical path through which reflected light including the fluorescence of the light enters the spectrophotometer element 15 of the light receiving unit 14 from the measurement hole 10a.
Further, the conical inner surface of the mounting member 11 facing the measurement hole 10a is provided with a black color, thereby absorbing the irregular reflection of the irradiation light from the light source as in the contact cover 10 described above. At the same time, consideration is given so that diffuse reflection of reflected light from the skin can be absorbed. Note that this color is not limited to black, and it is preferable to select a color that can effectively absorb irregular reflections according to light used as a light source and reflected light from the skin.

The LED light emitting unit 12 will be described in more detail. Regarding the relationship with a specific wavelength as an excitation light source for specific AGEs, in the present embodiment, for example, specific AGEs emit fluorescence such as crosslin, pyropyridine, and pentidine. AGEs are targeted, but not limited thereto, for example, AGEs produced as saccharification reaction intermediates such as 3 deoxyglucosone (3DG), and AGEs that do not emit fluorescence such as carboxymethyllysine (CML) Even so, the correlation between the specific excitation light wavelength of 370 nm for these AGEs and the corresponding fluorescence wavelength of 440 nm derived from AGEs so that the AGEs emitting fluorescence generated simultaneously in the generation process are also targeted. Focusing on the relationship, specifically, for example, the measurement head of the measurement device As the LED light emitting unit 12 in FIG. 10, an ultraviolet light LED having a wavelength of 365 nm slightly shorter than the specific excitation light wavelength of 370 nm and an ultraviolet light LED having a slightly longer wavelength of 375 nm are combined and adopted as an excitation light source, As shown in FIG. 5, in the state of being alternately arranged on the same circumference, they are embedded and fixed in the mounting member 11 (see FIG. 6), and two types of wavelengths close to these specific excitation lights are used. As described above, the excitation light from the ultraviolet LEDs 12a and 12b is concentrated and focused on one point of the measurement hole 10a at the center of the contact cover 10, and one LED light is weak. But it is gathered into one point and becomes a powerful light.
In addition, in principle, the two types of fluorescence emission derived from AGEs from the skin by these two types of ultraviolet light LEDs 12a and 12b correspond to the fluorescence of specific AGEs corresponding to the types of specific wavelengths of ultraviolet light LEDs. Excited, the same number of fluorescent emissions are generated as reflected light, and even AGEs that do not emit fluorescence are reflected from the saccharification reaction intermediate, or incidentally generate fluorescence during the AGE generation process, etc. Alternatively, it is possible to detect and measure a wide range of all reflected light such as fluorescent light emission derived from AGEs having higher fluorescence characteristics by any one of the ultraviolet LEDs 12a and 12b.
Further, regarding the selection of the wavelengths of these ultraviolet LEDs 12a and 12b, if the specific AGEs to be measured are different, the wavelength used as the excitation light is naturally relatively different, and the fluorescence wavelength derived from AGEs is also the above Needless to say, it is also efficient to select and adopt various kinds of ultraviolet LEDs according to the required wavelength, or to set slightly short wavelengths and long wavelength widths for specific excitation light wavelengths. The wavelength width is appropriately selected and set.

Further, the LED light emitting unit 12 is a white light LED 13 having a relatively long wavelength, for example, a wavelength of 465 nm in the case of this embodiment, for color correction such as variation in skin condition or absorbed light. As shown in FIG. 5, the ultraviolet light LEDs 12a and 12b are alternately arranged on the same circumference so as to face each other in an annular shape, and the white light LED 13 is mounted as shown in an enlarged view in FIG. In addition to being embedded and fixed in the member 11, the light emitting side mounting member 11 has an effect of suppressing the influence of excitation light other than the specific excitation light, for example, 380 nm or less, as the irradiation light to the skin tissue, A light emitting filter 13a made of colored glass that exhibits an effect for correcting shades of reflected light caused by variations in the state of the sun, red absorption light such as sunburn and melanin, etc. It has been kicked.

Furthermore, as a characteristic configuration of the present invention, the configuration of the light receiving portion 14 is important in combination with the LED light emitting portion 12 described above. That is, in the present invention, the central portion of the hollow conical mounting member 11 fixed in the measuring head main body 1a is in contact with the central portion of the contact cover 10 as shown in FIGS. The light receiving hole 14a is disposed so as to open on the same axis so as to face the measurement hole 10a, and the light receiving side attachment member 11 of the light receiving hole 14a has a skin tissue other than the fluorescence wavelength of 440 nm derived from the specific AGEs. The light receiving hole 14 is provided in the mounting member 11 on the back side of the light receiving hole 14a. Fluorescence derived from a specific AGE captured from 14a (in the illustrated example, two fluorescent emissions derived from AGE excited by two adjacent wavelengths of excitation light) Then, a base-integrated spectrophotometer element 15 that superimposes and amplifies the two measurement lights and directly converts them into an electrical signal for measurement is fixedly provided, and the fluorescence measurement value depends on the optical transmission means. Instead, it is configured to be converted into an electric signal and transmitted to the control board 2a of the operation box 2 via the cable 4.

Next, when using the measuring apparatus for the final saccharified product of the present invention, the measuring head 1, the operation box 2 and the personal computer 3 are electrically connected and set up as shown in FIG. Next, the operator who performs the measurement operation sets the change-over switches 2c, 2c and 2d for the power source and the light source to the appropriate input (ON) state, holds the measurement head 1 in his hand, and touches the optimal part such as the abdomen and neck of the subject. The contact cover 10 is pressed and this state is maintained for a while.
By doing so, the subject's skin is irradiated with 365 nm excitation light from the ultraviolet light LED 12a in the LED light emitting unit 12, 375 nm excitation light from the ultraviolet light LED 12b, and the white light LED 13 and is emitted by the light emission filter 13a. White light as filtered correction light for the skin is simultaneously irradiated at a pin point from the measurement hole 10a of the pressing surface.

At this time, if the skin tissue of the subject is in a good state such as a race difference, sex difference, extreme age difference, extreme sunburn state, or no melanin pigment deposition, the white light LED 13 does not need to emit light. Therefore, at that time, the changeover switch 2d of the operation box 2 may be turned OFF to stop the light emission of the white light LED 13, but in general, in order to obtain uniform measurement data under a certain condition, the white LED 13 is used. It is desirable to always perform color correction and make the measurement data only the fluorescent component.

Further, in the present embodiment, as the excitation light in the LED light emitting unit 12, the ultraviolet light LED 12a having a specific wavelength of 365 nm and the ultraviolet light LED 12b having a wavelength of 375 nm are simultaneously and concentratedly applied to the skin from the measurement hole 10a on the pressing surface. Since it is irradiated at a pinpoint, two fluorescences near the wavelength of 440 nm derived from AGEs in response to the specific excitation light are emitted from the skin that has received excitation light having these two specific wavelengths close to each other. These two types of fluorescence are emitted from the specific AGEs by the light receiving filter 14b of the light receiving unit 14 disposed in the one-pass type optical path that is oppositely arranged on the center line of the measuring head main body 1a. The reflected light from the skin other than the wavelength of 440 nm and the fluorescence from other substances are filtered and further passed through the light receiving hole 14a and the like. As shown in FIG. 9, the two fluorescent lights that enter the spectrophotometer element 15 and have a wavelength close to each other are converted into electric signals that have been amplified by polymerization and are not affected by noise or the like. It is reliably measured as a spectrophotometric distribution measurement value by the base-integrated spectrophotometer element 15, and this fluorescence measurement value is further transmitted via the cable 4 and processed by the control base 2 a of the operation box 2 after the operation. .

At this time, the ultraviolet light LED 12a and the ultraviolet light 12b of two types of wavelengths are set to the simultaneous input state as described above by the changeover switches 2c and 2c of the operation box 2, or any of the ultraviolet light LEDs 12a and 12b. When fluorescence emission derived from AGEs having high fluorescence characteristics due to one of them is remarkable and can be detected and measured stably, one of the input states is selected by the changeover switches 2c and 2c, and the ultraviolet having the higher fluorescence characteristics is selected. The light LEDs 12a and 12b can be operated to be selectively used. Further, the plurality of ultraviolet light LEDs (excitation light LEDs) provided in the LED light emitting unit 12 are specific one type for specific AGEs. If there are multiple devices that emit excitation light, select some of them to adjust the intensity of the irradiation light. It is also possible to.

As described above, according to the embodiment of the measuring apparatus whose overall structure is shown in FIG. 1, the existing general personal computer 3 is used to electrically connect the measuring head 1 and the operation box 2 of the present invention. Since the system is configured by cable connection, the entire system is compact and convenient to carry, and the measuring head 1 is shaped like a cup so that it can be grasped by hand. In this case, it can be measured easily and freely against the optimal part of the skin of the subject, and the operability is good. Further, the initial purpose of this type of device is not to place a burden on the human body. It is possible to measure glycated end product substances (AGEs) invasively.

In particular, on the same circumference of the inner conical surface of the mounting member 11 in the measuring head main body 1a, two kinds of ultraviolet LEDs 12a having different wavelengths close to a specific wavelength as an excitation light source for specific AGEs, 12b and white light LEDs 13 for color correction of the skin are alternately arranged in an annular shape, and an LED light emitting unit 12 is provided. Excitations emitted from the ultraviolet light LEDs 12a and 12b and the white light LED 13 are provided. Since the light and the correction light are focused and focused on one point of the measurement hole 10a at the center of the contact cover 10, the light and the correction light are condensed as irradiation light while being an energy saving type LED light. At the same time, in the light receiving portion 14 provided at the center of the conical top portion of the mounting member 11, the measurement hole 10a of the contact cover 10 and In a one-pass optical path by the light receiving hole 14a and the light receiving filter 14b arranged in the direction, the reflected light including the fluorescence from the skin enters the spectrophotometer element 15 from the measurement hole 10a, and is derived from two adjacent types of AGEs The fluorescence emission of the above is amplified and superposed and is directly converted into an electrical signal by the spectrophotometer element 15 and measured, so that the accuracy is very good.
In particular, the ultraviolet LEDs 12a and 12b having different wavelengths corresponding to specific wavelengths as excitation light sources for specific AGEs are not limited to two types,
If necessary, two or more types of ultraviolet light LEDs can be combined and used as an excitation light source. Fluorescence emission derived from specific AGEs due to the excitation light from these two or more types of ultraviolet light LEDs is also natural. Of course, two or more types are generated and can be strengthened by exhibiting a polymerization effect.

Further, in the present invention, a spectrophotometer element built in the measurement head main body 1a without using a general optical light path or optical conduction means that guides fluorescence emission derived from AGEs in a light state. 15, the fluorescent emission can be directly converted into an electric signal and detected, and this can be transmitted to the control board 2 a in the operation box 2 and the personal computer 3 as a judgment processing system, so that it is an inexpensive and simple device. It can be carried and excels in hot pullability.

  In the case of FIG. 1, the AGEs measuring device and system are configured using the operation box 2 and an existing general personal computer 3, but the operation of the control board 2 a and changeover switches 2 c and 2 d of the operation box 2 is performed. The function can be given to the personal computer 3 or can be changed so that it can be operated by hand by giving it to the measuring head 1 side. In this case, the measuring head 1 is the essential configuration of the present invention as described above. The light condensing arrangement structure of the LED light emitting unit 12 in the inside, the one-pass type optical path structure of the measurement hole 10a and the light receiving hole 14a of the light receiving unit 14, and the base-integrated spectrophotometer element 15 and the electric cable 4 are used. This can be carried out without any change and without changing the function and effect.

  As described above, the measurement apparatus for saccharification end product substances (AGEs) of the present invention includes a condensing arrangement structure obtained by arranging a plurality of light emitting LEDs in an LED light emitting portion inside the measurement head in an annular shape, and a measurement hole. As long as this is not changed, a plurality of light-emitting LEDs are specified as long as the combination structure such as a one-pass light path between the light-receiving part and the light-receiving part and an electrical conversion measurement method using a base-integrated spectrophotometer element in the light-receiving part is not changed. One type or two or more types having an excitation wavelength may be used. In addition, a computer or personal computer having an operation box or a display screen may have a shape structure other than the illustrated embodiment. In addition, the external shape of the measuring head itself is not limited to a further reduced pencil shape, and can be easily changed by those skilled in the art. In a possible range, it is free to various combinations or changes.

  Furthermore, with regard to specific glycation end products (AGEs), AGEs that emit fluorescence such as crosslin, pyropyridine, and pentodine described in the examples are targeted, or 3 deoxyglucosone (3DG) is used as a saccharification reaction intermediate. It is not limited to AGEs that generate fluorescence, and AGEs that generate fluorescence simultaneously with the generation process of AGEs that do not emit fluorescence, such as carboxymethyllysine (CML), and are used as an excitation light source for a specific AGE. The relationship between the specific wavelengths is not limited to the specific excitation light wavelength of 370 nm specifically described in the embodiment and the corresponding AGE-derived fluorescence wavelength of 440 nm. If the specific AGE changes, Of course, the wavelength of the excitation light LED as the excitation light source can be variously changed. Along with this change, the cut wavelength of the light receiving filter provided in the light receiving section is also changed as necessary, and is not limited to the wavelength of the embodiment, and the method of the light emitting filter and the light receiving filter is also used. It is not limited to the colored glass system.

This is the end of the description of the embodiment of the present invention. However, the scope of the present invention is not limited to the details shown or described, and may be implemented in various combinations. If it is possible and does not depart from the object of the present invention in any case, the excellent effects of the present invention can be exhibited without impairing.

  INDUSTRIAL APPLICATION This invention is utilized in the measurement field | area of a glycation end product (AGEs) in a medical field, a test | inspection technical field, especially the industrial field of preventive medicine.

DESCRIPTION OF SYMBOLS 1 Measuring head 1a Measuring head main body 2 Operation box 2a Control board 2b Power supply connector 2c, 2c, 2d changeover switch 3 Personal computer (judgment processing control part)
3a data display screen
4 Cable 4a, 4b Feeding cable 4c Wiring cable 5 USB cable 10 Contact cover 10a measurement hole
11 Mounting member 12 LED light emission part 12a, 12b UV light LED
13 White light LED
13a Light-emitting filter 14 Light-receiving part 14a Light-receiving hole 14b Light-receiving filter 15 Base-integrated spectrophotometer element

Claims (5)

The shape of the measuring head of the measuring device is made small and compact so that it can be grasped by a human hand, and a mounting member with a substantially hollow conical shape is provided inside the measuring head, on the same circumference of the conical inner surface A plurality of LEDs as light sources are arranged in a ring shape, and an LED light emitting unit is provided in which these LED lights are concentrated and directed at one point on the central axis, and a light receiving unit is provided at the central top of the mounting member. The base-integrated spectrophotometer elements are integrated and housed, and the light receiving part and the measurement hole opened in the center of the end face of the measuring head are arranged on the same axis, and the excitation light from the LED light emitting part Is condensed and irradiated to the skin from the measurement hole, and at the same time, a reflected light from the skin enters the light receiving part through the one-pass type optical path. ) Derived fluorescence The spectrophotometer element of the light receiving unit directly converts it into an electric signal and detects it, and transmits the electric signal related to the fluorescence measurement value to a control board in a separate operation box. The saccharification final product, characterized in that the LED light-emitting unit and the spectrophotometer element of the light-receiving unit can be controlled, and an electric signal related to the fluorescence measurement value is output as an input signal to the determination processing control unit Measuring device.   The shape of the measuring head of the measuring device is made small and compact so that it can be grasped by a human hand, and a mounting member with a substantially hollow conical shape is provided inside the measuring head, on the same circumference of the conical inner surface A plurality of LEDs as a light source are arranged in an annular shape, and an LED light emitting unit is provided in which these LED lights are concentrated and directed to one point on the center line, and a light receiving unit is provided at the central top of the mounting member. The substrate-integrated spectrophotometer elements are gathered and housed, and the light receiving part and the measurement hole opened in the center of the end face of the measurement head are arranged on the same axis, so that the excitation light from the LED light emitting part At the same time that the skin is condensed and irradiated from the measurement hole, a one-pass type optical path is formed so that the reflected light from the skin enters the light receiving unit through the measurement hole, and as the LED light emitting unit, Special for specific AGEs Two kinds of ultraviolet light LEDs, which are a combination of two or more kinds of ultraviolet light LEDs that emit excitation light, or a slightly shorter wavelength ultraviolet light LED and a slightly longer wavelength ultraviolet light LED than the excitation light for specific AGEs An excitation light source composed of a combination of the above, and two or more kinds of fluorescent light emission derived from AGEs from the skin by these two or more kinds of ultraviolet LED were polymerized and amplified by the spectrophotometer element of the light receiving section. The measured value is converted into an electric signal and detected, and this electric signal is transmitted to a control board in a separate operation box via a cable, and the LED light emitting unit and the spectrophotometer element of the light receiving unit are connected by the control board. The saccharification process is characterized in that an electric signal related to the fluorescence measurement value is output as an input signal to the determination processing control unit. Measuring device of the generated material. The LED light emitting unit as a light source in the measurement head is composed of an ultraviolet LED having a wavelength of 365 nm slightly shorter than a specific excitation light wavelength 370 nm and an ultraviolet LED having a slightly longer wavelength 375 nm for a specific glycation end product (AGEs). The apparatus for measuring a final saccharification product according to claim 1 or 2, wherein two adjacent ultraviolet light LEDs having different wavelengths are used as an excitation light source. The LED light-emitting unit as a light source in the measuring head includes a white light LED having a relatively long wavelength in addition to an excitation light source formed by combining a plurality of ultraviolet LEDs. The apparatus for measuring a saccharified final product according to any one of Items 2 and 3. The LED light emitting unit as a light source in the measurement head has a plurality of ultraviolet LEDs or two or more types of ultraviolet LEDs that emit specific excitation light for specific AGEs, and has high fluorescence characteristics due to any excitation light LED 2. The fluorescent light emission derived from the other AGEs is made selectable, and the fluorescent light emission is selectively converted into a measurement value electric signal by a spectrophotometer element of a light receiving section and can be detected. Or the measuring apparatus of the saccharification end product substance of Claim 2, Claim 3, and Claim 4.