JP2013520989A - ゲノムのクローニングおよび操作のための方法 - Google Patents

ゲノムのクローニングおよび操作のための方法 Download PDF

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JP2013520989A
JP2013520989A JP2012556048A JP2012556048A JP2013520989A JP 2013520989 A JP2013520989 A JP 2013520989A JP 2012556048 A JP2012556048 A JP 2012556048A JP 2012556048 A JP2012556048 A JP 2012556048A JP 2013520989 A JP2013520989 A JP 2013520989A
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genome
donor
cell
host
nucleic acid
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グウィネッド エー． ベンダーズ
ジョン アイ． グラス
クライド エー． ハッチソン
キャロル ラーティグ
サンジェイ バシー
ミッケル エー． アルギア
ハミルトン オー． スミス
チャールズ イー． メリーマン
ウラディミール エヌ． ノスコフ
レイ−ヤン チャン
ダニエル ジー． ギブソン
ジェイ． クレイグ ベンター
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シンセティック ジェノミクス インコーポレーテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
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  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2012556048A 2010-03-05 2010-05-19 ゲノムのクローニングおよび操作のための方法 Pending JP2013520989A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US12/718,911 2010-03-05
US12/718,911 US9273310B2 (en) 2007-10-08 2010-03-05 Methods for cloning and manipulating genomes
US32226910P 2010-04-08 2010-04-08
US61/322,269 2010-04-08
PCT/US2010/035490 WO2011109031A1 (fr) 2010-03-05 2010-05-19 Procédés de clonage et de manipulation de génomes

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JP2013520989A true JP2013520989A (ja) 2013-06-10

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EP (1) EP2542676A1 (fr)
JP (1) JP2013520989A (fr)
CN (1) CN102939380A (fr)
WO (1) WO2011109031A1 (fr)

Cited By (1)

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JP2016523559A (ja) * 2013-07-09 2016-08-12 プレジデント アンド フェローズ オブ ハーバード カレッジ 多重ｒｎａ誘導型ゲノム編集

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Cited By (6)

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JP2016523559A (ja) * 2013-07-09 2016-08-12 プレジデント アンド フェローズ オブ ハーバード カレッジ 多重ｒｎａ誘導型ゲノム編集
JP2020062044A (ja) * 2013-07-09 2020-04-23 プレジデント アンド フェローズ オブ ハーバード カレッジ 多重ｒｎａ誘導型ゲノム編集
JP7120717B2 (ja) 2013-07-09 2022-08-17 プレジデント アンド フェローズ オブ ハーバード カレッジ 多重ｒｎａ誘導型ゲノム編集
US11459585B2 (en) 2013-07-09 2022-10-04 President And Fellows Of Harvard College Multiplex RNA-guided genome engineering
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JP2023021447A (ja) * 2013-07-09 2023-02-10 プレジデント アンド フェローズ オブ ハーバード カレッジ 多重ｒｎａ誘導型ゲノム編集

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WO2011109031A8 (fr) 2012-09-20
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EP2542676A1 (fr) 2013-01-09

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