JP2013238797A5

JP2013238797A5 -

Info

Publication number: JP2013238797A5
Application number: JP2012113011A
Authority: JP
Filing date: 2012-05-17
Publication date: 2015-05-28
Anticipated expiration: 2032-05-17

Description

本発明の第１の態様は、蛍光観察と位相物体を可視化する観察とが行われる顕微鏡であって、対物レンズと、前記対物レンズの射出瞳面若しくは前記射出瞳面と共役な面又はそれらの近傍に配置された、光を変調する薄膜と、を含む結像光学系と、前記位相物体を励起する励起光と前記位相物体を可視化するための照明光とを前記薄膜と共役な面から出射する光源部とコンデンサレンズとからなる透過照明光学系と、を備え、前記光源部は、前記薄膜と共役な面のうちの前記薄膜と共役な領域内から前記照明光を出射するように構成される顕微鏡を提供する。
A first aspect of the present invention is a microscope in which fluorescence observation and observation for visualizing a phase object are performed, and includes an objective lens, an exit pupil plane of the objective lens, a plane conjugate with the exit pupil plane, or a surface thereof. An imaging optical system including a thin film that modulates light disposed in the vicinity, excitation light for exciting the phase object, and illumination light for visualizing the phase object are emitted from a surface conjugate with the thin film. A transmission illumination optical system including a light source unit and a condenser lens, and the light source unit is configured to emit the illumination light from within a region conjugated with the thin film in a surface conjugated with the thin film. Provide a microscope.

Claims

A microscope in which fluorescence observation and observation to visualize a phase object are performed,
An imaging optical system including an objective lens, and an exit pupil plane of the objective lens, a conjugate plane with the exit pupil plane, or a thin film that modulates light disposed in the vicinity thereof;
A transmission illumination optical system comprising a light source unit that emits excitation light for exciting the phase object and illumination light for visualizing the phase object from a surface conjugate with the thin film, and a condenser lens ;
The light source unit is configured to emit the illumination light from a region conjugated with the thin film in a surface conjugated with the thin film.

The microscope according to claim 1,
The light source unit is
A first light source unit that emits the illumination light from a region conjugated with the thin film in a surface conjugated with the thin film;
And a second light source unit that emits the excitation light having a wavelength different from that of the illumination light from outside the region conjugated with the thin film in a surface conjugated with the thin film.

The microscope according to claim 2,
The wavelength of the illumination light is longer than the wavelength of the excitation light and the wavelength of fluorescence generated by irradiation of the excitation light,
The imaging optical system further includes a barrier filter having a wavelength characteristic that blocks the excitation light and transmits the fluorescence and the illumination light.

The microscope according to claim 3,
The imaging optical system further includes an optical path branching unit that separates the fluorescence and the illumination light and guides them in different directions
A first photodetector for detecting the fluorescence separated by the optical path branching means;
And a second photodetector for detecting the illumination light separated by the optical path branching means.

In the microscope according to any one of claims 2 to 4,
The first light source unit is a first surface light source disposed in a region conjugated with the thin film among surfaces conjugated with the thin film,
The microscope according to claim 2, wherein the second light source unit is a second surface light source disposed outside a region conjugated with the thin film in a surface conjugated with the thin film.

In the microscope according to any one of claims 2 to 4,
The first light source unit emits the illumination light incident on the first optical fiber, and a first optical fiber disposed so that an emission end is positioned on a plane conjugate with the thin film. A light source, and
The second light source unit is configured to emit a second optical fiber disposed so that an emission end is positioned on a plane conjugate with the thin film, and a second light that emits the excitation light incident on the second optical fiber. A light source, and
The microscope, wherein the first optical fiber and the second optical fiber are bundled on an emission end side.

The microscope according to claim 1,
The light source unit is configured to emit the illumination light and excitation light having the same wavelength as the illumination light from a region conjugated with the thin film in a plane conjugate with the thin film. microscope.

The microscope according to claim 7,
The imaging optical system further includes a barrier filter having a wavelength characteristic that blocks the excitation light and transmits fluorescence generated by irradiation of the excitation light.

The microscope according to claim 8,
The microscope, wherein the barrier filter is detachably disposed on an optical axis of the objective lens.

The microscope according to claim 9,
The barrier filter is detachably disposed on an exit pupil plane of the objective lens or a plane conjugate with the exit pupil plane or in the vicinity thereof,
The microscope, wherein the barrier filter and the thin film are exchanged.

The microscope according to claim 8,
The imaging optical system further includes an optical path branching unit that separates the fluorescence and the illumination light and guides them in different directions
A first photodetector for detecting the fluorescence separated by the optical path branching means;
And a second photodetector for detecting the illumination light separated by the optical path branching means.

The microscope according to any one of claims 7 to 11,
The microscope, wherein the light source unit is a surface light source arranged in a region conjugate with the thin film on the emission surface.

The microscope according to any one of claims 7 to 11,
The light source unit includes an optical fiber disposed so that an emission end is positioned on a plane conjugate with the thin film, and a light source that emits the illumination light and the excitation light incident on the optical fiber,
The said light source part radiate | emits the said illumination light from the area | region conjugate with the said thin film among the said end surfaces of the said optical fiber, The microscope characterized by the above-mentioned.