JP2013231852A - Inverted type microscope - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an inverted type microscope in which a body unit and an observation unit can be attached easily and highly accurately.SOLUTION: An inverted type microscope 1 for imaging observation light from a sample S comprises: a body unit 10 including a primary imaging optical system forming the observation light of the sample S into an intermediate mage P1; a mirror 221 configured to bend observation light; a relay optical system formed from a mirror 221 for bending observation light and a plurality of lenses configured to relay the intermediate image P1; an observation unit 20 freely attached to or detached from the body unit 10; and a cylindrical optical system held by the observation unit 20 and configured to image observation light passed through the relay optical system.

Description

  The present invention relates to an inverted microscope that observes a specimen by irradiating the specimen with illumination light and receiving light reflected or transmitted from the specimen.

  2. Description of the Related Art Conventionally, in the fields of medicine and biology, a microscope for illuminating and observing a specimen has been used for observing cells and the like. In the industrial field, microscopes are used for various purposes such as quality control of metal structures, research and development of new materials, inspection of electronic devices and magnetic heads, and the like. As observation of a sample by a microscope, observation by visual observation, imaging a sample image using an imaging element such as a CCD or CMOS image sensor, and displaying the captured image on a monitor is known.

  A conventional inverted microscope is the base of an inverted microscope, a main unit provided with a control board that controls the entire inverted microscope, and a mirror that is detachably provided on the main unit and has at least an eyepiece. An observation unit having a cylinder (see, for example, Patent Document 1). The main unit includes, for example, a stage on which a specimen is placed, a revolver that holds a plurality of objective lenses having different magnifications interchangeably with respect to the specimen, a first lamp house that emits epi-illumination light, and a microscope main body that is transmitted through. A second lamp house or the like that emits illumination light is attached. In the inverted microscope disclosed in Patent Document 1, an observation unit and a lamp house can be detachably connected to the main unit, and can be combined according to the application.

  Here, in the conventional inverted microscope, the observation light that has passed through the objective lens is imaged by the imaging lens, and the observation light enters the observation unit side from the main unit via a relay optical system composed of a plurality of lenses. Then, the image is formed again on the imaging lens on the observation unit side. The observer observes the specimen by viewing the observation image formed on the imaging lens on the observation unit side.

JP 2002-267940 A

  However, in the inverted microscope disclosed in Patent Document 1, in a state where the main unit and the observation unit are connected, the relay optical system is disposed so as to straddle between the main unit and the observation unit. Here, since it is necessary for the relay optical system to align the axes of the lenses, it is preferable to integrally fix a plurality of lenses as the relay optical system. That is, in the inverted microscope disclosed in Patent Document 1, for example, it is necessary to fix the relay optical system on the observation unit side, and to form a holding unit that holds the relay unit of the portion protruding from the observation unit on the main unit side. is there.

  When the relay optical system is attached to the holding unit on the main unit side, the axis of the relay optical system needs to coincide with the optical axis of the observation light that has passed through the imaging lens. For this reason, accuracy such as the forming position is required in the holding portion described above, and in actuality, it is necessary to adjust with high accuracy using a machine or the like in a factory. Further, in the inverted microscope disclosed in Patent Document 1, a relay optical system has to be arranged on the bottom surface of the main unit due to its configuration, and adjustment while visually observing with a lens barrel is difficult.

  The present invention has been made in view of the above, and an object of the present invention is to provide an inverted microscope capable of easily and accurately attaching a relay optical system to a main unit.

  In order to solve the above-described problems and achieve the object, an inverted microscope according to the present invention is an inverted microscope that forms an image of observation light from a specimen, and forms the observation light of the specimen as an intermediate image. An observation unit that includes a main unit including a primary imaging optical system, a bending optical element that bends the observation light, and a relay optical system that includes a plurality of lenses that relay the intermediate image, and is detachable from the main unit. And a lens barrel optical system that forms an image of observation light that is held by the main body unit or the observation unit and passes through the relay optical system.

  The inverted microscope according to the present invention is characterized in that, in the above invention, the relay optical system is provided integrally with the plurality of lenses positioned.

  In the inverted microscope according to the present invention as set forth in the invention described above, the lens barrel optical system is held by the observation unit.

  In the inverted microscope according to the present invention as set forth in the invention described above, the relay optical system is disposed along a wall surface of the observation unit that is in contact with the main unit.

  In the inverted microscope according to the present invention, the lens closest to the bending optical element among the plurality of lenses of the relay optical system is the focal point of the intermediate image from the imaging position of the intermediate image. It is arranged outside the depth range.

  Further, in the inverted microscope according to the present invention, in the above invention, when the focal position of the lens closest to the bending optical element among the plurality of lenses of the relay optical system is f and the numerical aperture is NA, The lens effective diameter d closest to the bending optical element among the plurality of lenses of the relay optical system satisfies the formula d = 2 × f × NA.

  In the inverted microscope according to the present invention as set forth in the invention described above, the lens closest to the bending optical element among the plurality of lenses of the relay optical system has an effective diameter of 25 mm or more. And

  In the inverted microscope according to the present invention as set forth in the invention described above, the lens barrel optical system includes a prism.

  The inverted microscope according to the present invention is characterized in that, in the above invention, a prism is disposed between the primary imaging optical system and the bending optical element.

  According to the present invention, a main body unit including a primary imaging optical system that forms observation light of a sample as an intermediate image, a bending optical element that bends observation light imaged by the primary imaging optical system, and a plurality of optical elements An observation unit that includes a lens and includes a relay optical system that relays an intermediate image. The observation unit is detachable with respect to the main unit, and a lens barrel that is held by the main unit or the observation unit and forms observation light that has passed through the relay optical system. With the optical system, the relay optical system can be easily and accurately attached to the main unit.

FIG. 1 is a schematic diagram showing the overall configuration of an inverted microscope according to an embodiment of the present invention. FIG. 2 is a schematic diagram illustrating a configuration of a main part of the inverted microscope according to the embodiment of the present invention. FIG. 3 is a schematic diagram illustrating a configuration of a main part of the inverted microscope according to the embodiment of the present invention. FIG. 4 is a schematic diagram illustrating a configuration of a main part of the inverted microscope according to the embodiment of the present invention. FIG. 5 is a schematic diagram showing a configuration of a main part of the inverted microscope according to the embodiment of the present invention.

  DESCRIPTION OF EMBODIMENTS Hereinafter, embodiments for carrying out the present invention will be described in detail with reference to the drawings. In addition, this invention is not limited by the following embodiment. The drawings referred to in the following description only schematically show the shape, size, and positional relationship so that the contents of the present invention can be understood. That is, the present invention is not limited only to the shape, size, and positional relationship illustrated in each drawing.

  First, an inverted microscope according to the present embodiment will be described in detail with reference to the drawings. FIG. 1 is a schematic diagram showing an overall configuration of an inverted microscope 1 according to the present embodiment. As shown in FIG. 1, an inverted microscope 1 is an inverted microscope for observing a specimen S by imaging observation light from a specimen S specifically stained with a fluorescent dye, for example. A main body unit 10 that forms the base of the inverted microscope 1 and an observation unit 20 that is detachably provided on the main body unit 10 and has a lens barrel 20a provided with at least an eyepiece.

  The main unit 10 is mounted on the mounting surface of the inverted microscope 1, and is supported by the base 11a having a mirror leg 11a1 and 11a2 each extending in a substantially columnar shape, and a mirror base 11a1 and 11a2. In addition, the main body 11 includes a revolver holding portion 11b that holds a revolver 13 described later, and a column 11c that extends from the revolver holding portion 11b in the direction of the optical axis N1 of an objective lens 13a described later. The base 11 a is provided with a control board 11 d that controls the entire inverted microscope 1. The control board 11d may relay power from the outside to each part, or may have a built-in power supply and relay to each part.

  In addition, the column 11c is provided with a capacitor 111 supported so as to be movable up and down along the optical axis N1 of the objective lens 13a. Further, the main body unit 10 is formed with an opening 11e through which the observation light of the specimen S is emitted, and the observation light is sent to the observation unit 20 through the opening 11e while being connected to the observation unit 20. It is done.

  The main unit 10 is attached to a revolver holding unit 11b. For example, a stage 12 on which the specimen S is placed, a revolver 13 that holds a plurality of objective lenses 13a having different magnifications interchangeably with respect to the specimen S, and a light source 14a. , 15a, the first lamp houses 14 and 15 for emitting epi-illumination light, the second lamp house 19 having a light source 19a, which emits transmitted illumination light, and the like are attached. The light from the second lamp house 19 is reflected toward the specimen S (objective lens 13a) by the mirror 19b.

  In addition, in the inner regions 11a3 and 11a4 formed by the mirror legs 11a1 and 11a2 of the base 11a, a mirror unit 16 that switches the optical path of the incident illumination light and the light reflected from the sample S or transmitted through the sample S is a first lamp. It is attached according to the houses 14 and 15. In addition, the base 11a internally forms an image of the light from the mirror unit 16 to form an intermediate image P1, and reflects the light from the imaging lens 17a in a predetermined direction (axis N2 direction). Between the mirror 17b, the imaging lens 17a and the mirror 17b, provided in the optional unit additional region 112 disposed at a position crossing the optical axis N1, the optical axis N1 passing through the specimen S and the objective lens 13a. An optical path switching prism 18 that transmits or bends light in a predetermined direction. The primary imaging optical system may be configured by only the imaging lens 17a or may be formed by a plurality of lenses. The primary imaging optical system includes an imaging lens 17a and a mirror 17b. The optical path switching prism 18 is applicable as long as it is disposed between the imaging lens 17a and a mirror 221 described later.

  The revolver 13 is fixedly arranged with a plurality of objective lenses 13a having different magnifications, and by itself rotating, the objective lens 13a that is alternatively selected is arranged immediately below the specimen S placed on the stage 12. Thus, the predetermined objective lens 13a is made to irradiate the sample S with light or take in light from the sample S. Further, the revolver 13 is held by the revolver drive unit 131, and the revolver drive unit 131 approaches and separates from the stage 12 in accordance with, for example, power input via a rack-pinion mechanism (not shown). By displacing along the vertical direction, it can move along the optical axis N of the objective lens 13a. By this movement, the focus of the image of the sample S can be adjusted.

  In addition to transmitting power input via a rack-pinion mechanism, the revolver 13 may move under the control of the control board 11d by incorporating a motor.

  Further, a Z drift compensator (ZDC) (not shown) may be attached to the revolver holding portion 11b. ZDC is an active automatic focusing device that emits IR light (infrared rays) in the direction of the axis N3, inserts IR light in the direction of the optical axis N1 by the dichroic mirror 13b for ZDC, reflects from the sample S, and returns. The received IR light is returned again into the ZDC by the ZDC dichroic mirror 13b, and the position of the objective lens 13a and the sample S can be kept constant at the position of the return light. An observer can use the ZDC dichroic mirror 13b in the optical axis during long-time observation, etc., and use the ZDC to always focus the sample S and offset the objective lens 13a. .

  The first lamp houses 14 and 15 are housings incorporating light sources 14a and 15a such as mercury lamps, and are arranged in such a manner that they can be attached to and detached from the base 11a via the light projecting tubes 14b and 15b. The light projecting tubes 14b and 15b allow passage of light emitted from the light sources 14a and 15a of the first lamp houses 14 and 15. The light sources 14a and 15a may be replaced with lasers.

  The mirror unit 16 includes an excitation filter 16a, a dichroic mirror 16b, and an absorption filter 16c. The excitation filter 16a extracts light corresponding to the excitation wavelength from the emitted light emitted from the light sources 14a and 15a. The dichroic mirror 16b reflects light having a predetermined wavelength among the light emitted from the light sources 14a and 15a, and transmits light having a predetermined wavelength among the light emitted from the sample S. The absorption filter 16c extracts light having a desired wavelength from the light emitted from the sample S. A plurality of, for example, eight mirror units 16 are removably held in the mirror cassette, and light emitted from the light sources 14a and 15a of the first lamp houses 14 and 15 by a built-in motor (not shown). It is configured to be insertable / removable in a manner of entering or leaving the optical path.

  The mirror unit 16 has different functions of the excitation filter 16a, the dichroic mirror 16b, and the absorption filter 16c depending on the wavelengths of light emitted from the light sources 14a and 15a and the specimen S to be attached. By using the mirror unit 16 having different functions, for example, a fluorescent dye that stains a cell or a wavelength that excites a fluorescent protein that is expressed in the cell is selectively illuminated and double or triple stained cells Only the desired fluorescent dye or fluorescent protein can be excited.

  The optical path switching prism 18 has a plurality of prisms, for example, a prism 18a that transmits 100% of the light of the optical axis N1, transmits 50% of the light of the optical axis N1, and the remaining light of the optical axis N1. 50% of the light is bent in a direction orthogonal to the optical axis N1 (for example, a direction orthogonal to the paper surface), and 100% of the light of the optical axis N1 is orthogonal to the optical axis N1 (for example, the paper surface). And a prism 18c that bends in a direction orthogonal to the direction. Further, a camera port CP connected to a CCD camera or the like is provided at a position corresponding to the optical path switching prism 18 of the base 11a, and the light of the optical axis N1 is bent in a direction perpendicular to the optical axis N1 by the optical path switching prism 18. The emitted light is introduced into this camera port CP. Here, when the observation light passes through the prisms 18a to 18c, the distance traveled through the space in the observation light path can be shortened.

  The second lamp house 19 is a housing with a built-in light source 19a such as a halogen lamp, and is supported by the column 11c. The second lamp house 19 emits transmitted illumination light that passes through the sample S into the support column 11c. The mirror 19b provided inside the column 11c reflects the light emitted from the light source 19a of the second lamp house 19 to the sample S side.

  Here, the observation light from the specimen S reflected in the direction of the axis N2 by the mirror 17b forms an intermediate image P1. When the optical path is branched to the camera port CP side, an observation image is obtained at the same distance as the distance from this branch point to the image formation plane of the intermediate image P1. The base 11a is formed with a scale 113 for measuring a partial dimension of the sample S and a slot 113 for inserting a framing reticle indicating an imaging range at a position where the intermediate image P1 is formed.

  The observation unit 20 takes in the observation light of the sample S from the opening 11 e provided in the base 11 a while being connected to the main unit 10. The observation unit 20 has a main body 21 to which a lens barrel 20a having at least an eyepiece is detachably attached. Further, the main body portion 21 is formed with a protruding portion 21a that protrudes from the wall surface of the main body portion 21 and fits with the opening portion 11e.

  Inside the main body 21, the mirror 221 (bending optical element) that folds the observation light of the sample S emitted from the opening 11e and the light reflected by the mirror 221 are captured, and the intermediate image P1 is relayed. A plurality of lenses for parallel light are held by a holding cylinder 222 that is integrally held in a state of being positioned so that the centers of the respective lenses coincide with each other, a relay lens group 22 that forms a relay optical system, and a relay lens A first prism 23 that bends the intermediate image P1 relayed by the group 22 in a predetermined direction.

  The lens barrel 20a attached to the main body 21 has an imaging lens 24 that forms the observation light (parallel light) emitted from the first prism 23, and the observation light imaged by the imaging lens 24 in a predetermined direction. A second prism 25 that bends the The eyepiece lens of the lens barrel 20a forms the observation image P2 with the observation light emitted from the second prism 25.

  The imaging lens 24, the second prism 25, and the eyepiece lens 20a constitute a lens barrel optical system. The plurality of lenses held in the holding cylinder 222 may have an adjustment mechanism that can adjust the position of each lens alone. Further, each lens of the relay lens group 22 may be a condensing lens of the same magnification, may be a lens having a different magnification, or may be a zoom lens capable of switching the magnification. . Further, in the present embodiment, the description will be made assuming that it is held on the observation unit 20 side, but it may be held by the main unit 10 as long as it can be optically connected to the first prism 23.

  At this time, in the relay lens group 22, the lens 22 a closest to the mirror 221 (main unit 10) among the plurality of lenses captures the observation light reflected by the mirror 221 more reliably. It is preferable that the diameter of the light passes through the central axis of light and is perpendicular to the central axis. The diameter of the lens 22a is determined according to the distance from the imaging lens 17a in a state where the main unit 10 and the observation unit 20 are connected, for example. Here, when the focal position of the lens 22a is f and the numerical aperture is NA, the effective diameter d of the lens 22a satisfies the formula d = 2 × f × NA. Moreover, as an effective diameter of the lens 22a when actually used, for example, the effective diameter is preferably 25 mm or more.

  The lens 22a is disposed outside the focal depth range of the intermediate image P1 from the image formation position of the intermediate image P1. Thereby, even if a bubble exists in the optical member, the bubble does not appear in the observed image, and a sample image of the sample S can be obtained.

  In the inverted microscope 1 having the above-described configuration, in the case of epi-illumination, epi-illumination light from the light sources 14a and 15a is converted into parallel light beams in the light projection tubes 14b and 15b, the wavelength is selected by the excitation filter 16a, and the dichroic mirror 16b. As a result, the illumination light is bent toward the objective lens 13a. When the illumination light bent by the dichroic mirror 16b is applied to the sample S through the objective lens 13a, the sample S emits fluorescence by exciting the fluorescent dye or fluorescent protein of the cell. The objective lens 13a captures the emitted fluorescence as an image, passes through the dichroic mirror 16b and the absorption filter 16c, and an intermediate image P1 of the sample S positioned on the optical axis N1 is obtained by the objective lens 13a and the imaging lens 17a. It is formed on the observation optical path (on the optical axis N2), imaged at the position of the eyepiece through the relay lens group 22 and the imaging lens 24, and visually observed by the observer as a sample image P2 by emission of fluorescence or the like. .

  In the case of transmitted illumination, when the sample S is irradiated with transmitted illumination light from the light source 19a of the second lamp house 19 via the mirror 19b, an intermediate image of the sample S positioned on the optical axis N1 of the objective lens 13a. P1 is formed on the observation optical path (on the optical axis N2) by the objective lens 13a and the imaging lens 17a, imaged at the position of the eyepiece through the relay lens group 22 and the imaging lens 24, and a specimen image by transmitted light. P2 is visually observed by an observer. Transmitted light observation is used when performing bright field observation, phase difference observation, differential interference observation, and the like.

  2 to 5 are schematic diagrams illustrating the configuration of the main part of the inverted microscope 1 according to the present embodiment. 2 and 4 show the observation unit 20 with the lens barrel 20a removed. The main body unit 10 (base portion 11a) has the above-described opening 11e and a substantially rectangular opening on the surface that comes into contact with the observation unit 20 when connected to the observation unit 20, and forms a concave space. And a recess 11f. Further, the main body unit 10 (base portion 11a) is provided with a through hole 11g that is provided on a side surface orthogonal to the surface in contact with the observation unit 20 and penetrates toward the opening portion 11e.

  The observation unit 20 (main body portion 21) includes a protruding portion 21a that protrudes in a shape corresponding to the internal space formed by the opening 11e, and a protruding portion that protrudes in a protruding shape corresponding to the space formed by the recessed portion 11f. 21b.

  The opening 11e opens in a circular shape on the wall surface, and forms a weight-like space whose diameter is partially enlarged from the wall surface side toward the inner side. Moreover, the protrusion part 21a has the outer peripheral surface which expands according to the opening part 11e, and has comprised the weight shape which a protrusion direction side expands in diameter.

  The recess 11f forms a substantially columnar space whose diameter is partially enlarged from the wall surface side toward the inner side. Moreover, the convex part 21b has the outer peripheral surface which expands in diameter according to the recessed part 11f, and has comprised the column shape which a protrusion direction side expands in diameter.

  When connecting the main body unit 10 and the observation unit 20, the protrusion part 21a is fitted with respect to the opening part 11e. At this time, the convex portion 21b and the concave portion 11f are fitted to each other, so that rotation around the opening 11e of the observation unit 20 with respect to the main body unit 10 is prevented, and the attachment position of the observation unit 20 with respect to the main body unit 10 is determined. decide. Thereby, an optical path between the mirrors 17b and 221 is formed, and the specimen image P2 by the observation light of the specimen S can be formed on the eyepiece.

  Here, as shown in FIG. 5, with the opening 11e and the protrusion 21a fitted, the screw 100 is inserted into the through hole 11g and fixed to the side surface of the protrusion 21a. The connection state of the main unit 10 and the observation unit 20 can be further ensured. Further, when the size of the space of the opening 11e is larger than the outer periphery of the protrusion 21a, the connection state of the main body unit 10 and the observation unit 20 is achieved by bringing the protrusion 21a into contact with the wall surface of the opening 11e with the screw 100. And positioning of the observation unit 20 can be performed.

  Also, instead of the observation unit 20, a sample image can be taken by connecting a camera unit having an image sensor such as a CCD or CMOS to the opening 11e. At this time, it can be realized by adjusting the optical path length according to the distance between the imaging lens 17a and the specimen image P2 and forming an image on the light receiving surface of the image sensor. Here, when visual observation and observation by imaging are performed at the same time, the optical path switching prism 18 transmits 50% of the light of the optical axis N1 and the remaining 50% of the light of the optical axis N1. By switching to the prism 18b that bends in a direction orthogonal to the optical axis N1 and connecting the camera unit to the camera port CP, it is possible to observe simultaneously.

  According to the present embodiment described above, the relay lens group 22 and the mirror 221 which are composed of a plurality of lenses and are used in a state in which the center of each lens coincides are accommodated in the observation unit 20, via the mirrors 17 b and 221. Since the observation light from the main unit 10 is taken in and the observation unit 20 can be attached to and detached from the main unit 10, the main unit and the observation unit can be easily and accurately attached. As a result, the relay optical system can be easily and accurately attached to the main unit.

  Further, according to the above-described embodiment, the main body unit 10 does not require a space for disposing the relay optical system, and the main body unit 10 can be made the minimum necessary size. It is possible to minimize the area occupied by the microscope. Furthermore, in this minimum necessary configuration, the observation unit and the camera unit can be selectively attached.

  Here, in the conventional inverted microscope, an intermediate image obtained by forming the observation light of the specimen is bent once and bent to relay the eyepiece by the relay optical system, and the intermediate image of the specimen is bent twice. And a U-shaped optical path that relays to the eyepiece by a relay optical system. In general, the V-shaped optical path that relays the intermediate image to the eyepiece after being bent once is shorter in optical path length than the U-shaped optical path that is bent twice, so that a bright and less deteriorated image can be obtained. Has been. On the other hand, when a mirror unit or the like is disposed between the objective lens 13a and the imaging lens 17a, in the V-shaped optical path, the mirror unit is disposed in order to prevent the mirror unit from being disposed on the optical path. Therefore, a U-shaped optical path is preferable.

  On the other hand, according to the embodiment described above, the diameter of the lens 22a is set according to the distance from the imaging lens 17a, and the light reflected by the lens 221 is captured in a wider range. Thus, the observation light from the specimen S can be reliably collected and a clear specimen image can be obtained. That is, it is possible to obtain an inverted microscope having both the characteristics of a V-shaped optical path and a U-shaped optical path.

  Further, according to the above-described embodiment, a plurality of prisms are provided on the optical path of the observation light so as to fill the space on the optical path. The optical path that travels inside can be shortened, and a bright and less deteriorated image can be obtained in the U-shaped optical path configuration. In addition, by reflecting the observation light in the prism and switching the optical path, it is not necessary to arrange a plurality of mirrors according to the path, and the configuration can be simplified.

  In addition, according to the above-described embodiment, the relay lens group 22 is disposed along the wall surface of the observation unit 20 that contacts the main body unit 10, thereby suppressing an increase in the size of the observation unit 20 itself. In addition, it is possible to suppress an increase in the size of the entire inverted microscope 1 when the observation unit 20 is attached to the main unit 10. Further, since the relay lens group 22 is fixed in a state where a plurality of lens groups are positioned, adjustment such as optical axis alignment can be easily performed.

  In the above-described embodiment, the inverted microscope has been described as an example. For example, an objective lens that forms a differential interference observation optical system and enlarges the specimen, an imaging function that images the specimen via the objective lens, and an image The present invention can also be applied to an imaging device having a display function for displaying the image, such as a video microscope.

  As described above, the inverted microscope according to the present invention is useful for easily attaching the main unit and the observation unit with high accuracy.

DESCRIPTION OF SYMBOLS 1 Inverted microscope 10 Main body unit 11,21 Main body part 11a Base part 11a1, 11a2 Mirror leg part 11b Revolver holding part 11c Support | pillar 11d Control board 11e Opening part 11f Recessed part 11g Through-hole 12 Stage 13 Revolver 13a Objective lens 13b, 16b Dichroic 14 , 15 First lamp house 14a, 15a, 19a Light source 16 Mirror unit 16a Excitation filter 16c Absorption filter 17a, 24 Imaging lens 17b, 19b, 221 Mirror 18 Optical path switching prism 19 Second lamp house 20 Observation unit 20a Lens barrel 21a Projection Part 21b Convex part 22 Relay lens group 23 First prism 25 Second prism 111 Condenser 112 Optional unit additional area 113 Slot

Claims (9)

An inverted microscope that images observation light from a specimen,
A body unit including a primary imaging optical system that forms observation light of the specimen as an intermediate image;
A bending optical element that bends the observation light, and a relay optical system that includes a plurality of lenses that relay the intermediate image, and an observation unit that is detachable from the body unit;
A lens barrel optical system that forms an image of observation light that is held by the main unit or the observation unit and passes through the relay optical system;
An inverted microscope characterized by comprising:   The inverted microscope according to claim 1, wherein the relay optical system is integrally provided with the plurality of lenses positioned.   The inverted microscope according to claim 2, wherein the lens barrel optical system is held by the observation unit.   The inverted microscope according to claim 2, wherein the relay optical system is disposed along a wall surface of the observation unit that is in contact with the main body unit.   The lens closest to the folding optical element among the plurality of lenses of the relay optical system is disposed outside the focal depth range of the intermediate image from the imaging position of the intermediate image. Inverted microscope. Of the plurality of lenses of the relay optical system, when the focal position of the lens closest to the bending optical element is f and the numerical aperture is NA,
2. The inverted microscope according to claim 1, wherein among the plurality of lenses of the relay optical system, an effective lens diameter d closest to the bending optical element satisfies an expression d = 2 × f × NA.   The inverted lens according to any one of claims 1 to 6, wherein, among the plurality of lenses of the relay optical system, the lens closest to the bending optical element has an effective diameter of 25 mm or more. Type microscope.   The inverted microscope according to claim 1, wherein the lens barrel optical system includes a prism.   The inverted microscope according to claim 1, wherein a prism is disposed between the primary imaging optical system and the bending optical element.

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