JP2013225061A5 - - Google Patents

Download PDF

Info

Publication number
JP2013225061A5
JP2013225061A5 JP2012097780A JP2012097780A JP2013225061A5 JP 2013225061 A5 JP2013225061 A5 JP 2013225061A5 JP 2012097780 A JP2012097780 A JP 2012097780A JP 2012097780 A JP2012097780 A JP 2012097780A JP 2013225061 A5 JP2013225061 A5 JP 2013225061A5
Authority
JP
Japan
Prior art keywords
wavelength
observation
light
unit
illumination light
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2012097780A
Other languages
Japanese (ja)
Other versions
JP2013225061A (en
JP5969803B2 (en
Filing date
Publication date
Application filed filed Critical
Priority to JP2012097780A priority Critical patent/JP5969803B2/en
Priority claimed from JP2012097780A external-priority patent/JP5969803B2/en
Publication of JP2013225061A publication Critical patent/JP2013225061A/en
Publication of JP2013225061A5 publication Critical patent/JP2013225061A5/ja
Application granted granted Critical
Publication of JP5969803B2 publication Critical patent/JP5969803B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Claims (5)

照明光を発する光源と、該光源から発せられた前記照明光が照射されることにより標本から戻る戻り光を検出する検出部と、前記照明光を前記光源から前記標本に導くまたは前記戻り光を前記標本から前記検出部に導く光学素子とを備える、選択的に切替可能な複数の観察ユニットと、
切替前の前記観察ユニットにより前記標本に照射されていた前記照明光の波長および前記検出部によって検出されていた前記戻り光の波長を推定し、切替前の前記観察ユニットと切替後の前記観察ユニットとの間で前記照明光の波長および前記戻り光の波長がそれぞれ略一致するように、推定した前記照明光の波長および前記戻り光の波長に基づいて、切替後の前記観察ユニットにおける前記光学素子の透過または反射に関する波長特性を設定する制御部とを備える顕微鏡装置。 A light source that emits illumination light, a detection unit that detects return light that is returned from the specimen by being irradiated with the illumination light emitted from the light source, and that guides the illumination light from the light source to the specimen or the return light. A plurality of observation units selectively switchable, comprising optical elements guided from the specimen to the detection unit;
Estimating the wavelength of the illumination light applied to the specimen by the observation unit before switching and the wavelength of the return light detected by the detection unit, and the observation unit before switching and the observation unit after switching The optical element in the observation unit after switching is based on the estimated wavelength of the illumination light and the wavelength of the return light so that the wavelength of the illumination light and the wavelength of the return light substantially match each other. And a control unit that sets a wavelength characteristic related to transmission or reflection of the microscope. 前記切替前の観察ユニットにおける光学素子の波長特性を記憶する記憶部を備え、
前記制御部が、前記記憶部に記憶されている前記切替前の観察ユニットにおける光学素子の波長特性から前記照明光および前記戻り光の波長特性を推定する請求項１に記載の顕微鏡装置。 A storage unit that stores the wavelength characteristics of the optical element in the observation unit before switching,
The microscope apparatus according to claim 1, wherein the control unit estimates wavelength characteristics of the illumination light and the return light from wavelength characteristics of an optical element in the observation unit before switching stored in the storage unit. 選択的に切替可能な複数の前記観察ユニットのうち、少なくとも１つの前記観察ユニットの光源が、他の前記観察ユニットの光源としても機能するようになっている請求項１または２に記載の顕微鏡装置。3. The microscope apparatus according to claim 1, wherein a light source of at least one of the observation units among a plurality of the selectively switchable observation units also functions as a light source of another observation unit. . 選択的に切替可能な複数の前記観察ユニットのうち、少なくとも１つの前記観察ユニットが、蛍光を波長ごとに分光するグレーティングを備える請求項１から３いずれかに記載の顕微鏡装置。The microscope apparatus according to any one of claims 1 to 3, wherein at least one of the plurality of observation units that can be selectively switched includes a grating that separates fluorescence for each wavelength. 選択的に切替可能な複数の前記観察ユニットのうち、少なくとも１つの前記観察ユニットは、前記検出部がマルチチャンネルPMTであり、蛍光を波長ごとに分光するグレーティングを該マルチチャンネルPMTの手前に備える請求項１から３いずれかに記載の顕微鏡装置。At least one of the plurality of observation units that can be selectively switched, the detection unit is a multi-channel PMT, and a grating that separates fluorescence for each wavelength is provided in front of the multi-channel PMT. Item 4. The microscope apparatus according to any one of Items 1 to 3.

JP2012097780A 2012-04-23 2012-04-23 Microscope equipment Active JP5969803B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2012097780A JP5969803B2 (en) 2012-04-23 2012-04-23 Microscope equipment

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2012097780A JP5969803B2 (en) 2012-04-23 2012-04-23 Microscope equipment

Publications (3)

Publication Number Publication Date
JP2013225061A JP2013225061A (en) 2013-10-31
JP2013225061A5 true JP2013225061A5 (en) 2015-05-28
JP5969803B2 JP5969803B2 (en) 2016-08-17

Family

ID=49595144

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2012097780A Active JP5969803B2 (en) 2012-04-23 2012-04-23 Microscope equipment

Country Status (1)

Country Link
JP (1) JP5969803B2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20220155577A1 (en) * 2019-03-28 2022-05-19 Hamamatsu Photonics K.K. Confocal microscope unit and confocal microscope

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4756819B2 (en) * 2003-10-21 2011-08-24 オリンパス株式会社 Scanning microscope system
WO2006049180A1 (en) * 2004-11-01 2006-05-11 Olympus Corporation Emission measuring instrument and emission measuring method
JP4645176B2 (en) * 2004-11-30 2011-03-09 株式会社ニコン Spectroscopic microscope
JP5131071B2 (en) * 2008-07-18 2013-01-30 株式会社ニコン Fluorescence microscope apparatus and focus detection apparatus
JP5415854B2 (en) * 2009-07-10 2014-02-12 オリンパス株式会社 Spectroscopic apparatus and scanning microscope apparatus
JP5371694B2 (en) * 2009-10-26 2013-12-18 オリンパス株式会社 Microscope connection unit and microscope system
JP5452180B2 (en) * 2009-11-13 2014-03-26 オリンパス株式会社 Microscope equipment

Similar Documents

Publication Publication Date Title
ES2582331T3 (en) Line Conveyor Device
EP3731724A4 (en) Fluorescence imaging in a light deficient environment
WO2014176375A3 (en) Systems and methods for recording simultaneously visible light image and infrared light image from fluorophores
ES2478693A1 (en) Element for blocking short wavelengths in led-type light sources
JP2011530718A5 (en)
EP2776875A4 (en) Optical sheet, display device and light emitting device having the same
EP2568320A3 (en) Vehicle lamp
BR112014013873A8 (en) ophthalmic filter
WO2015173695A3 (en) Display device, rear view device and motor vehicle
CL2008003026A1 (en) Device for determining an optical property of a sample with a tunable excitation light source for applying light to the sample and a detector for capturing light exiting the sample, wherein the light source is an array of emitting diodes of the light very close to each other, and the method to use the device
EP2663609A4 (en) Compound for organic optoelectronic device, organic light emitting diode including the same and display including the organic light emitting diode
TWI562995B (en) Material for organic optoelectronic device, organic light emitting diode including the same and display including the organic light emitting diode
WO2014105470A8 (en) Multifunctional lightguide taillight article
EP2778846A3 (en) Eye/gaze tracker and method of tracking the position of an eye and/or a gaze point of a subject
EP2657998A3 (en) Semiconductor light emitting device
EP3403126A4 (en) Systems and methods for instant total internal reflection fluorescence/structured illumination microscopy
WO2012119114A3 (en) E-petri dishes, devices, and systems
DE602008001948D1 (en) Tire with component containing aramid fibers
WO2016023846A3 (en) An injection device and a supplemental device configured for attachment thereto
ES2721646T3 (en) Device for optoelectronic detection of a selection lever position, selection lever device, manufacturing procedure of a device and procedure for optoelectronic detection of a selection lever position
WO2014195917A3 (en) Detector-array-based sample characterization
EP2685496A3 (en) Light emitting apparatus
JP2013238797A5 (en)
EP2737345A4 (en) Optical member, display device and light emitting device having the same
EP3986235A4 (en) Fluorescence imaging in a light deficient environment