JP2013221763A - Spectroscopic analyzer, spectroscopic analysis method and program for spectroscopic analyzer - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a spectroscopic analyzer, a spectral analysis method and a program for the spectroscopic analyzer capable of calculating proper stray light quantity for every wavelength when a plurality of light-receiving elements receive light of every wavelength.SOLUTION: A stray light rate calculation unit 42 calculates a stray light rate at a specific wavelength on the basis of light quantity at the specific wavelength calculated by a light quantity calculation unit 41 when no sample absorbing light is on a light path and light quantity at the specific wavelength calculated by the light quantity calculation unit 41 when light is transmitted through a sample absorbing light of the specific wavelength. A stray light calculation unit 43 calculates stray light quantity of every wavelength by calculation using the stray light rates at every wavelength and characteristic values which are different at every wavelength (for example, coefficients which are different at every wavelength stored in a coefficient storage unit 53).

Description

  The present invention relates to a spectroscopic analysis apparatus, a spectroscopic analysis method, and a spectroscopic analysis program for performing analysis by separating light transmitted through a sample on an optical path with a spectroscope and receiving light of each wavelength with different light receiving elements. Is.

  Some spectroscopic analyzers can receive light from a spectroscope using a plurality of light receiving elements such as photodiode arrays. In this type of spectroscopic analyzer, light of each wavelength dispersed by the spectroscope is received by a light receiving element corresponding to each wavelength, whereby the amount of light for each wavelength can be measured.

  The light received by each light receiving element in the spectroscopic analysis apparatus as described above is not necessarily only the light transmitted through the sample and spectrally separated by the spectroscope. For example, when light that has not entered the spectroscope or light that has been reflected by various optical components in the spectroscopic analyzer (so-called stray light) enters the light receiving element, it is greater than the amount of light that has passed through the sample. The amount of light is received by the light receiving element.

  The influence of such stray light on the analysis is particularly remarkable in a wavelength region where the absorbance of the sample is high, that is, in a wavelength region where the amount of light received by the light receiving element is small. In a configuration in which light of each wavelength is received by a plurality of light receiving elements, the influence of stray light may be affected by the position of each of the light receiving elements.

  In order to remove the influence of the stray light as described above on the analysis, the stray light amount is generally measured (for example, see Patent Document 1 below). Patent Document 1 discloses that the amount of stray light is measured in a short wavelength region where the absorbance of the sample is high (transmittance is low) (paragraph [0004] and the like).

JP-A-10-170339

  For example, if the measured stray light amount is used as a correction value and correction is performed by subtracting the correction value from the amount of light received by each light receiving element, the influence of stray light on the analysis can be suppressed. However, since the amount of received light differs for each wavelength (for each light receiving element), there is a possibility that the influence of stray light on the analysis cannot be effectively suppressed only by subtracting a certain amount of stray light from the amount of light received by each light receiving element. .

  FIG. 6 is a diagram for explaining the influence of stray light on the analysis, and shows the relationship between the original absorbance and the absorbance obtained from the measurement results. The straight line shown by the solid line in this figure shows the relationship between the original absorbance at the reference wavelength (the wavelength when the stray light amount is measured) and the absorbance obtained from the measurement result.

  In the wavelength region where the absorbance is higher than the reference wavelength, that is, the wavelength region where the amount of received light is small, the ratio of the constant stray light amount to the amount of received light increases. For this reason, as a result of the constant stray light amount being too large as a correction value, the linearity of absorbance decreases as indicated by a two-dot chain line in the figure. On the other hand, in the wavelength region where the absorbance is lower than the reference wavelength, that is, the wavelength region where the amount of received light is large, the ratio of the constant stray light amount to the amount of received light is small. Therefore, as a result of the constant stray light amount being too small as a correction value, the linearity of absorbance is lowered as shown by a broken line in the figure.

  The present invention has been made in view of the above circumstances, and a spectroscopic analysis apparatus and a spectroscopic analysis method capable of calculating an appropriate amount of stray light for each wavelength when a plurality of light receiving elements receive light of each wavelength. Another object of the present invention is to provide a program for a spectroscopic analyzer. It is another object of the present invention to provide a spectroscopic analyzer, a spectroscopic analysis method, and a spectroscopic analyzer program that can easily calculate the amount of stray light at each wavelength. Furthermore, an object of the present invention is to provide a spectroscopic analysis device, a spectroscopic analysis method, and a program for the spectroscopic analysis device that can effectively suppress the influence of stray light on the analysis.

  The spectroscopic analyzer according to the present invention separates the light transmitted through the sample on the optical path with a spectroscope, and receives light of each wavelength with a different light receiving element, so that each signal is received based on the signal from each light receiving element. A spectroscopic analyzer that calculates a light amount of a wavelength by a light amount calculation unit, wherein the light amount of a specific wavelength calculated by the light amount calculation unit in a state where there is no sample having absorption on the optical path, and absorption at the specific wavelength A stray light rate calculating unit that calculates a stray light rate at the specific wavelength based on the light amount of the specific wavelength calculated by the light amount calculating unit when light is transmitted through the sample, and a stray light rate at the specific wavelength, and And a stray light amount calculation unit for calculating a stray light amount at each wavelength by a calculation using a unique value different for each wavelength.

  According to such a configuration, when light of each wavelength is received by a plurality of light receiving elements, an appropriate stray for each wavelength is obtained by calculation using a stray light rate at a specific wavelength and a unique value that differs for each wavelength. The amount of light can be calculated. That is, instead of applying the stray light amount by measurement of a specific wavelength uniformly as in the past, the stray light rate at a specific wavelength is calculated, and the calculation using the stray light rate is performed for each wavelength. The amount of light can be calculated suitably.

  The state in which there is no sample having absorption on the optical path includes a state in which only a solvent is present on the optical path and no sample is present, and a state in which no sample and solvent are present. For example, in the case of a configuration in which analysis is performed by introducing a solvent and a sample into the sample cell, a state where only the solvent is present in the sample cell or a state where the sample cell is empty is on the optical path. May be included in a state where there is no sample having absorption.

  The spectroscopic analyzer may further include a coefficient storage unit that stores a different coefficient for each wavelength calculated in advance as a ratio of the stray light amount at each wavelength to the stray light amount at the specific wavelength. In this case, the stray light amount calculation unit stores the stray light rate at the specific wavelength, the light amount of the specific wavelength calculated by the light amount calculation unit without a sample having absorption on the optical path, and the coefficient storage unit. The stray light amount at each wavelength may be calculated by calculation using a different coefficient for each stored wavelength.

  According to such a configuration, an appropriate stray light amount can be calculated for each wavelength by using a different coefficient for each wavelength calculated in advance as the ratio of the stray light amount at each wavelength to the stray light amount at the specific wavelength. . That is, since the stray light rate at a specific wavelength and the amount of stray light at the specific wavelength calculated by the light amount calculation unit in the state where there is no sample having absorption on the optical path, the stray light amount at the specific wavelength can be specified. Based on the stray light amount at the specific wavelength and the coefficient, the stray light amount at each wavelength can be suitably calculated.

  In particular, according to the above configuration, it is not necessary to measure the light amount of each wavelength, and the stray light amount at each wavelength can be calculated only by measuring the light amount of a specific wavelength. Therefore, the stray light amount at each wavelength can be easily calculated as compared with a configuration in which the stray light amount at each wavelength is calculated by measuring the light amount at each wavelength.

  In this case, the stray light rate at the specific wavelength is multiplied by the light amount of the specific wavelength calculated by the light amount calculation unit in the absence of a sample having absorption on the optical path, and further stored in the coefficient storage unit. A value obtained by multiplying a different coefficient for each wavelength may be calculated as the stray light amount at each wavelength.

  The stray light amount calculation unit is not limited to the configuration described above, and the stray light amount calculation unit is different in light amount for each wavelength calculated by the light amount calculation unit in a state where there is no sample having absorption on the optical path and the optical path. The amount of stray light at each wavelength may be calculated by a calculation using. In this case, the value obtained by multiplying the stray light rate at the specific wavelength by a different light amount for each wavelength calculated by the light amount calculation unit in the absence of a sample having absorption on the optical path is a stray light amount at each wavelength. May be calculated as

  When the spectroscopic analyzer transmits light to an arbitrary sample, the light amount of each wavelength received by each light receiving element, and the stray light amount at each wavelength calculated by the stray light amount calculation unit, You may further provide the light quantity correction | amendment part which correct | amends the light quantity of each wavelength.

  According to such a configuration, the light amount of each wavelength obtained by subtracting the appropriate stray light amount by correcting the light amount of each wavelength received by each light receiving element using the stray light amount calculated in advance for each wavelength as a correction value. Can be obtained. By performing the analysis using the light amounts of the respective wavelengths thus obtained, the influence of stray light on the analysis can be effectively suppressed.

  In the spectroscopic analysis method according to the present invention, the light transmitted through the sample on the optical path is dispersed by the spectroscope, and the light of each wavelength is received by the different light receiving elements. A spectral analysis method for calculating a light amount of a wavelength by a light amount calculation unit, wherein the light amount of a specific wavelength calculated by the light amount calculation unit in a state where there is no sample having absorption on the optical path, and absorption at the specific wavelength A stray light rate calculation step of calculating a stray light rate at the specific wavelength based on the light amount of the specific wavelength calculated by the light amount calculation unit when light is transmitted through the sample having the stray light rate at the specific wavelength; and And a stray light amount calculation step of calculating a stray light amount at each wavelength by a calculation using a unique value that differs for each wavelength.

  The program for a spectroscopic analysis device according to the present invention separates light transmitted through a sample on an optical path with a spectroscope, and receives light of each wavelength with a different light receiving element, so that it is based on a signal from each light receiving element. A program for a spectroscopic analyzer that calculates a light amount of each wavelength by a light amount calculation unit, the light amount of a specific wavelength calculated by the light amount calculation unit in a state where no sample has absorption on the optical path, and the specific A stray light rate calculating step for calculating a stray light rate at the specific wavelength based on the light amount of the specific wavelength calculated by the light amount calculation unit when light is transmitted through a sample having absorption at a wavelength; and at the specific wavelength A stray light amount calculation step for calculating a stray light amount at each wavelength by a calculation using a stray light rate and a specific value different for each wavelength. To.

  According to the present invention, when light of each wavelength is received by a plurality of light receiving elements, an appropriate stray light amount for each wavelength is obtained by calculation using a stray light rate at a specific wavelength and a unique value different for each wavelength. Can be calculated. Further, according to the present invention, it is not necessary to measure the light amount of each wavelength, and the stray light amount at each wavelength can be easily calculated only by measuring the light amount of a specific wavelength. Furthermore, according to the present invention, the effect of stray light on the analysis is effectively obtained by correcting the amount of light of each wavelength received by each light receiving element using an appropriate amount of stray light calculated in advance for each wavelength as a correction value. Can be suppressed.

It is the schematic which showed the structural example of the spectroscopic analyzer which concerns on one Embodiment of this invention. FIG. 2 is a block diagram showing an electrical configuration of the spectroscopic analyzer of FIG. 1. It is the flowchart which showed the flow of the process at the time of calculating the stray-light rate _RA . It is the flowchart which showed the flow at the time of correct | amending the light quantity I ((lambda)) of each wavelength at the time of an analysis. It is the flowchart which showed the flow at the time of correct | amending the light quantity I ((lambda)) of each wavelength at the time of analysis in the spectroscopic analyzer which concerns on another embodiment. It is a figure for demonstrating the influence which a stray light has on an analysis, and has shown the relationship between the original light absorbency and the light absorbency obtained from a measurement result.

  FIG. 1 is a schematic diagram illustrating a configuration example of a spectroscopic analyzer according to an embodiment of the present invention. The spectroscopic analysis apparatus includes an optical system 10 including a light source 1, a condenser 2, a slit plate 3, a spectroscope 4, a photodiode array 5, and the like. At the time of analysis, a sample cell 20 made of, for example, a flow cell is disposed on the optical path L formed by the optical system 10, and light such as white light is irradiated on the sample in the sample cell 20.

  The light emitted from the light source 1 is collected by, for example, a condenser 2 including a condenser lens, and is applied to the sample in the sample cell 20. Of the light that has passed through the sample, the light that has passed through the slit plate 3 is incident on the spectroscope 4 made of, for example, a diffraction grating. The spectroscope 4 decomposes the light transmitted through the sample into a spectrum for each wavelength.

  The photodiode array 5 includes a plurality of light receiving elements 6. The light of each wavelength separated by the spectroscope 4 is received by different light receiving elements 6 of the photodiode array 5. Therefore, the light quantity of each wavelength can be calculated based on the signal from each light receiving element 6.

  FIG. 2 is a block diagram showing an electrical configuration of the spectroscopic analyzer of FIG. As shown in FIG. 2, the spectroscopic analysis apparatus according to the present embodiment includes an A / D conversion unit 30, a data processing unit 40, a storage unit 50, and the like. The signal from element 6 can be processed.

  The A / D conversion unit 30 performs a process of converting an analog signal input from each light receiving element 6 of the photodiode array 5 into a digital signal. A signal from each light receiving element 6 converted into a digital signal by the A / D conversion unit 30 is input to the data processing unit 40.

  The data processing unit 40 includes, for example, a CPU (Central Processing Unit), and when the CPU executes a program, the light amount calculation unit 41, the stray light rate calculation unit 42, the stray light amount calculation unit 43, and the light amount correction unit 44. Function as such. The storage unit 50 can be configured by, for example, a ROM (Read-Only Memory) and a RAM (Random-Access Memory). In this example, a light amount storage unit 51, a stray light rate storage unit 52, a coefficient storage unit 53, a stray light amount storage unit 54, and the like are allocated to the storage unit 50.

  The light quantity calculation unit 41 calculates the light quantity of each wavelength based on the signal from each light receiving element 6 of the photodiode array 5. The light amount calculated by the light amount calculation unit 41 can be stored in the light amount storage unit 51 for each wavelength. In the stray light rate calculation unit 42, the stray light amount calculation unit 43, and the light amount correction unit 44, processing described later is performed using the light amount data stored in the light amount storage unit 51.

The stray light rate calculation unit 42 calculates the ratio of stray light at the specific wavelength λ _{A as} the stray light rate R _A. Prior to the processing by the stray light rate calculation unit 42, the light amount I _A of the specific wavelength λ _A is converted into the light amount calculation unit 41 in a state where a solvent is introduced into the sample cell 20 and there is no sample having absorption in the sample cell 20. Is calculated by Thereafter, the sample (hereinafter, referred to as "reference sample".) Having absorption in a specific wavelength lambda _A is is introduced into the sample cell 20, the light amount I _B of a particular wavelength lambda _A when light is transmitted to the reference sample Calculated by the light amount calculation unit 41.

The stray light rate R _A at the specific wavelength λ _A can be calculated by substituting the light amount I _A and the light amount I _B calculated as described above into the following equation (1). The stray light ratio R _A, in particular the wavelength lambda _A, the light amount I _A in each state of the state without a sample having an absorption in the sample cell 20, a state where there is a reference sample having an absorption in a specific wavelength lambda _A, and the ratio of I _B. The calculated stray light rate R _A at the specific wavelength λ _A is stored in the stray light rate storage unit 52.
R _A = I _B / I _A (1)

The stray light amount calculation unit 43 calculates the stray light amount at each wavelength by a calculation using the stray light rate R _{A at} the specific wavelength λ _A and a specific value different for each wavelength. In the present embodiment, a coefficient C (λ) for each wavelength stored in advance in the coefficient storage unit 53 is used as a unique value that is different for each wavelength. The coefficient storage unit 53, for example, as the ratio of the stray light amount at each wavelength for the amount of stray light at a particular wavelength lambda _A, factor different for each wavelength preliminarily calculated C (lambda) is stored. However, the unique value that differs for each wavelength is not limited to the coefficient C (λ) for each wavelength, but may be other values.

The process by the stray light amount calculation unit 43 is a process performed when analyzing an arbitrary sample, for example, separately from the process by the stray light rate calculation unit 42. At the time of analysis, first, prior to processing by the stray light amount calculation unit 43, the solvent is introduced into the sample cell 20, and there is no sample having absorption in the sample cell 20, the light amount I _C of the specific wavelength λ _A is Calculated by the light amount calculation unit 41. The light amount I _C of the specific wavelength λ _A calculated in this way, the stray light rate R _{A at the} specific wavelength λ _A stored in the stray light rate storage unit 52, and the wavelength stored in the coefficient storage unit 53 The stray light quantity S (λ) at each wavelength can be calculated by substituting a different coefficient C (λ) for each equation into the following equation (2).
S (λ) = I _C × R _A × C (λ) (2)

In the present embodiment, when light of each wavelength is received by the plurality of light receiving elements 6, the stray light rate R _A at the specific wavelength λ _A and the coefficient C (λ), which is a specific value different for each wavelength, are used. An appropriate stray light amount S (λ) can be calculated for each wavelength by calculation. That is, instead of applying the amount of stray light uniformly by conventional measurement of a specific wavelength as to calculate the stray ratio R _A at a particular wavelength lambda _A, carrying out the operation using the stray light ratio R _A for each wavelength Thus, the stray light amount S (λ) at each wavelength can be calculated suitably.

In this example, the stray light amount S (λ) appropriate for each wavelength is obtained by using a different coefficient C (λ) for each wavelength calculated in advance as the ratio of the stray light amount at each wavelength to the stray light amount at the specific wavelength λ _A. Can be calculated. That is, the stray light ratio R _A at a particular wavelength lambda _A, and, based on the amount I _C of a particular wavelength lambda _A calculated by the light amount calculating section 41 with no sample having an absorption in the light path L, the specific wavelength lambda _A it is possible to identify the amount of stray light in, it can be based on the amount of stray light and the coefficient C (lambda) at the specific wavelength lambda _a, suitably calculates the amount of stray light S (lambda) at each wavelength.

In particular, according to the above configuration, it is not necessary to measure the light amount of each wavelength, and the stray light amount S (λ) at each wavelength can be calculated only by measuring the light amount I _C of the specific wavelength λ _A. it can. Therefore, the stray light amount S (λ) at each wavelength can be easily calculated as compared with a configuration in which the stray light amount S (λ) at each wavelength is calculated by measuring the light amount at each wavelength.

The light quantity correction unit 44 performs processing for correcting the light quantity I (λ) of each wavelength calculated by the light quantity calculation unit 41 and stored in the light quantity storage unit 51. The process by the light quantity correction unit 44 is a process performed separately from the process by the stray light rate calculation unit 42 as in the process by the stray light quantity calculation unit 43, and is performed, for example, when analyzing an arbitrary sample. Specifically, when light is transmitted through an arbitrary sample, the light amount I (λ) of each wavelength received by each light receiving element 6 and the stray light amount at each wavelength calculated by the stray light amount calculation unit 43 By substituting S (λ) into the following equation (3), the corrected light amount I ′ (λ) of each wavelength from which the stray light amount S (λ) is subtracted can be calculated.
I ′ (λ) = I (λ) −S (λ) (3)

  In this way, by correcting the light quantity I (λ) of each wavelength received by each light receiving element 6 using the stray light quantity S (λ) calculated in advance for each wavelength as a correction value, an appropriate stray light quantity S ( A light amount I ′ (λ) of each wavelength obtained by subtracting λ) can be obtained. By performing analysis using the light amounts I ′ (λ) of the respective wavelengths obtained in this way, the influence of stray light on the analysis can be effectively suppressed.

FIG. 3 is a flowchart showing the flow of processing when calculating the stray light rate _RA . In the present embodiment, the stray light rate R _A is calculated in advance and stored in the stray light rate storage unit 52 prior to analysis of an arbitrary sample.

In calculating the stray light ratio _RA , first, a solvent is introduced into the sample cell 20 (step S101), and light is emitted from the light source 1 in a state where there is no sample having absorption in the sample cell 20. At this time, based on the amount of light received by the light receiving element 6 corresponding to a particular wavelength lambda _A, the amount of light I _A specific wavelength lambda _A is calculated (step S102). Thereafter, the light intensity I _B of a particular wavelength lambda _A at the time when the reference sample having an absorption in a specific wavelength lambda _A is introduced into the sample cell 20 (step S103), and transmits light to the reference sample is calculated (step S104). After the light amount I _A and the light amount I _B are calculated in this way, the values are substituted into the above-described equation (1), thereby calculating the stray light rate R _{A at the} specific wavelength λ _A (step) S105).

  These steps S101 to S105 may be configured to be performed automatically by executing a program, or may be configured to be performed only partly automatically. For example, a configuration in which at least one step is executed by a human may be used.

FIG. 4 is a flowchart showing a flow when correcting the light quantity I (λ) of each wavelength during analysis. When analyzing a sample, first, a solvent is introduced into the sample cell 20 (step S201), and light is emitted from the light source 1 in a state where there is no sample having absorption in the sample cell 20. At this time, based on the amount of light received by the light receiving element 6 corresponding to a particular wavelength lambda _A, the light amount I _C having a specific wavelength lambda _A is calculated (step S202). Then, the stray light rate _RA calculated in advance is read from the stray light rate storage unit 52, and a coefficient C (λ) that differs for each wavelength is read from the coefficient storage unit 53 (step S203).

Thereafter, the light amount I _C of the specific wavelength λ _A calculated in step S202, and the stray light rate R _A and the coefficient C (λ) read in step S203 are respectively substituted into the above-described equation (2). Thus, the stray light amount S (λ) at each wavelength is calculated (step S204). The stray light is obtained by correcting the stray light amount S (λ) at each wavelength calculated as described above as a correction value and subtracting the correction value from the light reception amount of each light receiving element 6 in subsequent steps S205 to S207. Can suppress the influence on the analysis.

  Specifically, first, an arbitrary sample to be analyzed is introduced into the sample cell 20 (step S205), and the sample is irradiated with light from the light source 1. The light of each wavelength transmitted through the sample and dispersed by the spectroscope 4 is received by each light receiving element 6, whereby the light amount I (λ) of each wavelength is calculated (step S206). The light quantity I (λ) of each wavelength calculated in this way and the stray light quantity S (λ) of each wavelength calculated in step S204 are respectively substituted into the above-described equation (3), whereby the stray light quantity. A corrected light quantity I ′ (λ) for each wavelength from which S (λ) has been subtracted is calculated (step S207). The calculated corrected light amount I ′ (λ) may be overwritten and stored on the light amount I (λ) of each wavelength stored in the light amount storage unit 51, or the light amount I (λ) of each wavelength. It may be stored separately.

  These steps S201 to S207 may be configured to be performed automatically by executing a program, or may be configured to be performed only partly automatically. For example, a configuration in which at least one step is executed by a human may be used.

FIG. 5 is a flowchart showing a flow when the light quantity I (λ) of each wavelength is corrected at the time of analysis in the spectroscopic analyzer according to another embodiment. In this embodiment, only the mode of processing by the stray light amount calculation unit 43 is different from the above embodiment, and the mode of processing by the stray light rate calculation unit 42 and the light amount correction unit 44 is the same as that of the above embodiment. Therefore, the stray light rate calculation unit 42 can calculate the stray light rate _RA by the process shown in FIG. 3 as in the above embodiment.

When analyzing a sample in the present embodiment, first, a solvent is introduced into the sample cell 20 (step S301), and light is emitted from the light source 1 in a state where there is no sample having absorption in the sample cell 20. . At this time, the light amount I _C (λ) of each wavelength is calculated based on the amount of light received by each light receiving element 6 (step S302). Thus, in the above embodiment, only the light amount I _C of the specific wavelength λ _A is calculated, whereas in the present embodiment, the light amount I _C (λ) of each wavelength is calculated. The light amount I _C (λ) of each wavelength calculated in this way is used for calculation in the stray light amount calculation unit 43 as a unique value that differs for each wavelength. That is, in the present embodiment, the stray light amount calculation unit 43 is different for each wavelength calculated by the light amount calculation unit 41 in a state where there is no sample having absorption on the optical path L and the stray light rate R _{A at} the specific wavelength λ _A. The stray light amount S (λ) at each wavelength is calculated by calculation using the light amount I _C (λ).

Specifically, after the light amount I _C (λ) of each wavelength is calculated as described above (step S302), the previously calculated stray light rate _RA is read from the stray light rate storage unit 52 (step S303). . Thereafter, the light amount I _C (λ) of each wavelength calculated in step S302 and the stray light rate R _A read in step S303 are substituted into the following formula (4), respectively, so that stray light at each wavelength is obtained. A light quantity S (λ) is calculated (step S304).
S (λ) = I _C (λ) × R _A (4)

  The stray light is obtained by correcting the stray light amount S (λ) at each wavelength calculated in this way as a correction value and subtracting the correction value from the light reception amount in each light receiving element 6 in subsequent steps S305 to S307. Can suppress the influence on the analysis.

  Specifically, first, an arbitrary sample to be analyzed is introduced into the sample cell 20 (step S305), and the sample is irradiated with light from the light source 1. The light of each wavelength transmitted through the sample and dispersed by the spectroscope 4 is received by each light receiving element 6, whereby the light amount I (λ) of each wavelength is calculated (step S306). The stray light amount I (λ) calculated for each wavelength calculated in this way and the stray light amount S (λ) for each wavelength calculated in step S304 are respectively substituted into the above-described equation (3). The corrected light quantity I ′ (λ) for each wavelength from which S (λ) has been subtracted is calculated (step S307). The calculated corrected light amount I ′ (λ) may be overwritten and stored on the light amount I (λ) of each wavelength stored in the light amount storage unit 51, or the light amount I (λ) of each wavelength. It may be stored separately.

  These steps S301 to S307 may be configured to be performed automatically by executing a program, or may be configured to be performed only partly automatically. For example, a configuration in which at least one step is executed by a human may be used.

In the above embodiment, the stray light rate calculation step for calculating the stray light rate R _A at the specific wavelength λ _{A and} the stray light amount calculation step for calculating the stray light amount S (λ) at each wavelength are both executed by the spectroscopic analyzer. The configuration as described above has been described. In this case, a program for causing a computer to execute each step may be provided separately from the spectroscopic analyzer. Thereby, the computer which controls using the said program functions as a spectroscopic analyzer, and there can exist an effect similar to the said embodiment. It is also possible to provide the program stored in a storage medium.

  The present invention can be applied to a spectroscopic analyzer such as a high performance liquid chromatograph. However, the present invention is not limited to a high-performance liquid chromatograph, and any other spectroscopic analysis is possible as long as it is a spectroscopic analyzer having a configuration in which light that has been transmitted through a sample and separated by a plurality of light receiving elements 6 is received. It can be applied to a device. In addition, the plurality of light receiving elements 6 are not limited to those configured by the photodiode array 5, and any other light receiving elements can be employed.

DESCRIPTION OF SYMBOLS 1 Light source 2 Condenser 3 Slit plate 4 Spectrometer 5 Photodiode array 6 Light receiving element 10 Optical system 20 Sample cell 30 A / D conversion part 40 Data processing part 41 Light quantity calculation part 42 Stray light rate calculation part 43 Stray light quantity calculation part 44 Light amount correction unit 50 Storage unit 51 Light amount storage unit 52 Stray light rate storage unit 53 Coefficient storage unit 54 Stray light amount storage unit

Claims (5)

The light transmitted through the sample on the optical path is dispersed by a spectroscope, and the light of each wavelength is received by different light receiving elements, and the light amount of each wavelength is calculated by the light quantity calculation unit based on the signal from each light receiving element. A spectroscopic analyzer that performs
Calculated by the light quantity calculation unit when light having a specific wavelength calculated by the light quantity calculation unit in a state where there is no sample having absorption on the optical path, and when light is transmitted through the sample having absorption at the specific wavelength. A stray light rate calculation unit for calculating a stray light rate at the specific wavelength based on the light amount of the specific wavelength;
A spectroscopic analyzer, comprising: a stray light amount calculation unit that calculates a stray light amount at each wavelength by a calculation using a stray light rate at the specific wavelength and a unique value different for each wavelength. As a ratio of the stray light amount at each wavelength to the stray light amount at the specific wavelength, a coefficient storage unit that stores a different coefficient for each wavelength calculated in advance,
The stray light amount calculation unit is stored in the coefficient storage unit, the stray light rate at the specific wavelength, the light amount of the specific wavelength calculated by the light amount calculation unit in the absence of a sample having absorption on the optical path, and the coefficient storage unit. The spectroscopic analyzer according to claim 1, wherein the stray light amount at each wavelength is calculated by a calculation using a different coefficient for each wavelength.   When light is transmitted through an arbitrary sample, the light quantity of each wavelength is corrected based on the light quantity of each wavelength received by each light receiving element and the stray light quantity at each wavelength calculated by the stray light quantity calculation unit. The spectroscopic analysis apparatus according to claim 1, further comprising a light amount correction unit that performs the operation. The light transmitted through the sample on the optical path is dispersed by a spectroscope, and the light of each wavelength is received by different light receiving elements, and the light amount of each wavelength is calculated by the light quantity calculation unit based on the signal from each light receiving element. A spectroscopic analysis method comprising:
Calculated by the light quantity calculation unit when light having a specific wavelength calculated by the light quantity calculation unit in a state where there is no sample having absorption on the optical path, and when light is transmitted through the sample having absorption at the specific wavelength. A stray light rate calculating step for calculating a stray light rate at the specific wavelength based on the light amount of the specific wavelength;
And a stray light amount calculating step of calculating a stray light amount at each wavelength by a calculation using a stray light rate at the specific wavelength and a unique value different for each wavelength. The light transmitted through the sample on the optical path is dispersed by a spectroscope, and the light of each wavelength is received by different light receiving elements, and the light amount of each wavelength is calculated by the light quantity calculation unit based on the signal from each light receiving element. A program for a spectroscopic analyzer,
Calculated by the light quantity calculation unit when light having a specific wavelength calculated by the light quantity calculation unit in a state where there is no sample having absorption on the optical path, and when light is transmitted through the sample having absorption at the specific wavelength. A stray light rate calculating step for calculating a stray light rate at the specific wavelength based on the light amount of the specific wavelength;
A program for a spectroscopic analyzer, which causes a computer to execute a stray light amount calculation step of calculating a stray light amount at each wavelength by a calculation using a stray light rate at the specific wavelength and a unique value different for each wavelength. .

Images