KR20110097250A - Spectrophotometer apparatus using color filter array - Google Patents
Spectrophotometer apparatus using color filter array Download PDFInfo
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- KR20110097250A KR20110097250A KR1020100017006A KR20100017006A KR20110097250A KR 20110097250 A KR20110097250 A KR 20110097250A KR 1020100017006 A KR1020100017006 A KR 1020100017006A KR 20100017006 A KR20100017006 A KR 20100017006A KR 20110097250 A KR20110097250 A KR 20110097250A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B5/00—Optical elements other than lenses
- G02B5/20—Filters
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- H04N5/335—
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- H04N5/374—
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- Investigating Or Analysing Materials By Optical Means (AREA)
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Abstract
The present invention relates to a spectroscopic analysis device and a density measuring method using the same, wherein a color filter array is used to obtain luminance characteristics of a specific wavelength passing through individual color filters, thereby simplifying the construction of the device and applying the wavelengths to multiple wavelengths at once. It is possible to detect the light intensity characteristic.
Description
The present invention relates to a spectrophotometric analysis of measuring the distribution according to the wavelength of light, to investigate the physical or chemical properties of the material, in particular by measuring the absorbance or transmittance of the sample material or the solution of the sample material, The present invention relates to a spectroscopic analyzer that can obtain the same characteristics.
Conventionally used spectroscopic analysis apparatus spectroscopy the light of a light source and irradiates a sample, receives the reflected light or transmitted light, and converts the received light into an amount of charge and measures the light.
The structural diagram of the conventional spectroscopic analyzer is shown as an example in FIG.
As shown in FIG. 11, the conventional spectroscopic analyzer includes a
The
The
The
The
Since the monochromator used in the conventional spectroscopic analyzer is mainly using a prism or a diffraction grating, its structure is complicated and a device for driving is required. In addition, even when the wavelength is selected using the filter, a driving device such as a filter wheel is required, and since the filter must be provided for each wavelength, the cost increases.
In addition, the conventional spectroscopic analyzer shows a large difference in measurement accuracy between a clean sample and a cloudy sample. For samples with high turbidity, a method of increasing the measurement accuracy through dilution may be considered, but there is a problem that may deviate from the detection limit.
The present invention has been made to solve the above problems, and to provide a spectroscopic analysis device and a concentration measuring method using the same, which is simple in structure, can correct the turbidity of the sample, and can be manufactured at low cost.
Spectroscopic analysis device according to the present invention for solving the above problems is a device for measuring the characteristics of the sample by transmitting light to the sample, the light source unit for generating light, and the light containing the cell containing the sample light generated in the light source unit A sample unit for transmitting the sample, a color filter array unit configured to arrange a plurality of color filters having a specific color, and selectively transmitting light having a specific wavelength for each color filter by passing the light passing through the sample; And a detection unit configured to detect light passing through the color filter array unit to detect optical characteristics of the sample.
The color filter array unit includes a diffuser for diffusing the light generated from the light source unit so that the light is uniformly irradiated onto the individual color filters, an infrared blocking lens for blocking infrared rays from the light incident through the diffuser; It may include a condenser lens for effectively inducing light in the individual color filters. The condenser lens may be composed of a plurality of micro lenses corresponding to individual color filters.
The detection unit detects light passing through the color filter array unit and converts it into an electrical signal, an analog-to-digital converter that converts the electrical signal from the image sensor into a digital signal, and processes the converted digital signal It may include an image signal processor for displaying the characteristics of the light passing through the sample. The image sensor may be configured as a charge-coupled device (CCD) or a complementary metal oxide semiconductor (CMOS).
The method for measuring the concentration of a sample using the spectroscopic analyzer includes measuring the amount of light (I Dark ) in a state where light is blocked, and obtaining a measured value (I Blank ) of each color filter for a reference sample. Wow. By transmitting light to the test specimen (sample) to determine the concentration, the measurement value for each color filter for the step of obtaining a measured value (I Sample) of each color filter, wherein the test sample for the test sample (I Sample) Comparing the amount of light attenuation for each color from the step, Obtaining the transmittance or absorbance for the test sample, Measuring the absorbance for the standard sample, Obtaining a calibration curve graph of the absorption and concentration, and the test Calculating the concentration of the test sample from the permeability or absorbance for the sample and the calibration curve graph.
The concentration measuring method may further include correcting turbidity of the test sample by using the measured value for the color of the color filter which does not affect the color of the test sample.
Since the spectroscopic analyzer of the present invention does not use a diffraction ball or a filter wheel, a driving unit is not necessary. Therefore, the structure is simple and manufacturing cost can be low. In addition, there is no need for balanced light, and all of the materials that can be colored have an effect that can be measured. In addition, turbidity correction can be simultaneously performed, and the sensitivity of RGB can be controlled by software, thereby extending the range of measurement density.
1 is a schematic configuration diagram of a spectroscopic analysis device according to an embodiment of the present invention.
2 is a cross-sectional view showing an example of implementing a CFA and an image sensor in the spectroscopic analysis device according to an embodiment of the present invention.
3 is a flowchart illustrating a method of measuring concentration by a spectroscopic analysis device according to an embodiment of the present invention.
4 is a graph showing the RGB intensity of the reference sample measured according to the concentration measuring method according to an embodiment of the present invention.
5 is a graph showing the RGB intensity of the test sample measured according to the concentration measuring method according to an embodiment of the present invention.
6 (a) and 6 (b) is the wavelength-specific sensitivity of the CFA used in the test measured according to the concentration measuring method according to an embodiment of the present invention and the intensity of each wavelength of the light source used, Figure 6 (c) And (d) is a graph showing absorbance and transmittance for each wavelength in the test measured according to the concentration measuring method according to the embodiment of the present invention.
7 is a graph measuring the absorbance of R of the test sample measured according to the concentration measuring method according to an embodiment of the present invention.
8 is a graph showing a calibration curve of a standard sample measured according to the concentration measuring method according to the embodiment of the present invention.
9 and 10 are graphs showing measurement results of absorbance when turbidity correction is not performed and turbidity correction is performed on samples having the same concentration according to the concentration measuring method according to the embodiment of the present invention.
It is a block diagram which shows an example of the conventional spectroscopic analyzer.
Hereinafter, with reference to the accompanying drawings, a preferred embodiment of the present invention will be described.
1 is a block diagram of a spectroscopic analysis device according to an embodiment of the present invention. As shown in FIG. 1, the spectroscopic analyzer of the present invention includes a
The
The
The color
The color
The
2 illustrates an example of implementing a CFA and an image sensor in more detail.
As shown in Fig. 2, on the
Although not shown in the drawings, the output signal of the
The spectroscopic analyzer of the present invention described above operates as follows.
First, the light from the
That is, in the present invention, instead of using a diffraction grating or changing filters to select a specific wavelength, the CFA can be used to obtain luminous intensity characteristics for a specific wavelength passing through individual color filters. In this way, the intensity for each wavelength passing through the color filter is obtained, and compared with the intensity for the sample to be compared, to obtain characteristics such as the concentration of the sample.
As described above, the spectroscopic analyzer of the present invention does not require a driving unit for driving the diffraction grating, a structure for replacing the filter, or the like, so that the configuration of the device is simple and very convenient to use. In addition, it is possible to detect the luminance characteristics for a plurality of wavelengths at a time, so that it can be combined appropriately to analyze more various characteristics for the sample.
The CFA used for the spectroscopic analyzer of the present invention may be used as it is already manufactured for components used in a digital camera, a flat panel display device, or the like, or may be made of a dedicated CFA to suit the purpose of the spectroscopic analyzer. The former has the advantage of lowering the manufacturing cost by using mass-produced parts, and the latter has the advantage of designing a spectroscopic analyzer that is optimal for use and characteristics.
Below, the density | concentration measuring method of a sample is demonstrated as an example of photometric measurement using the spectroscopic analyzer of this invention. 3 is a flowchart of the concentration measuring method by the spectroscopic analysis device of the present invention.
After preparing a series of standard samples in the concentration range to be measured and preparing a reference sample such as distilled water, measure the amount of light (I dark ) in a state where light is blocked by a spectroscopic analyzer (step 100).
The reference sample is mounted on the
A test sample to measure the concentration is mounted and a light source is operated to allow light to pass through the test sample , thereby obtaining an RGB measurement value I sample of the test sample (step 300).
Compare the amount of light attenuation of each of R, G, and B from the RGB measurement value (I sample ) of the test sample, and use the wavelength of the largest light attenuation (color with high absorption) as the main measurement source, Color having a small absorbance) is used as the reference color (step 400). For example, a sample that absorbs only R (red) compares the absorbance of R only, and when absorbing light in the middle region of R (red) and G (green), consider the absorbance of R and G at the same time. .
As measured in the area irrespective of the color of the solution, the measured part can be accurately obtained by setting this part as the reference light amount in relation to the turbidity of the solution.
Next, according to Lambert-Beer's law, the permeability (T) or the absorbance (A) of the test sample is obtained using the following equation (step 500).
T = (I Sample -I Dark ) / (I Blank -I Dark )
A = log (1 / T)
Absorbance for the standard sample is measured, and a calibration curve graph for absorbance and concentration is drawn (step 600).
After calculating the coefficient values (a, b, c, d) from the calibration curve graph, the absorbance (A) and the coefficient value are substituted into the concentration formula below to determine the concentration of the test sample (step 700).
Conc. = AA 3 + bA 2 + cA + d
4 to 8 show graphs of the results of measuring the concentration of the test sample using the concentration measuring method described above. At this time, the CFA used has an RGB pattern of 640x320 pixels.
First, FIG. 4 shows the RGB intensity (Light Intensity) of the reference sample obtained in
In addition, the absorbance for each wavelength was determined by comprehensively considering these values, the wavelength-specific sensitivity of each filter, and the waveform of the light source used, and are shown in FIG. 6. That is, FIGS. 6 (a) and 6 (b) are wavelength-specific sensitivity of CFA used in the test and wavelength-specific intensity of the light source used, and FIGS. 6 (c) and 6 (d) are absorbances at wavelength and Permeability is shown.
7 shows the absorbance for the R wavelength obtained in
In the spectroscopic analyzer of the present invention, the turbidity of a sample to be measured can be simultaneously corrected by using CFA. This turbidity correction method will be described below.
Usually, the measured absorbance depends on the difference in turbidity due to the particles contained in the sample solution. Conventionally, two light sources or two filters have to be used to compensate for this absorbance due to turbidity. Even in most water quality meters it is common to measure after removal of particles.
Since the CFA is used in the present invention, the effect of using several filters at the same time can be obtained. Therefore, the turbidity of the sample solution can be corrected using the measured value for any color of the CFA which has little effect on the color of the sample solution. Turbidity is perceived as absorption in most areas by reducing the amount of transmitted light. Therefore, the true value of absorption by color must be obtained. This can be solved by subtracting the unabsorbed region which is a reference from the absorption color.
9 and 10 show measurement results of absorbance when turbidity correction was performed on samples having the same concentration and without turbidity correction. 9 shows that the turbidity correction is not performed, and the difference in absorbance between the turbid sample and the clean sample is very large. On the other hand, FIG. 10 shows a case in which turbidity correction is performed, and the difference in absorbance between the clouded sample and the clean sample is significantly reduced compared to FIG. 9.
Although the present invention has been described with reference to the above-described preferred embodiments and the accompanying drawings, different embodiments may be constructed within the spirit and scope of the invention. Accordingly, the scope of the present invention is defined by the appended claims, and is not to be construed as limited to the specific embodiments described herein.
10
12
21
30
32 blocking
34, 53
41
43
51
54 micro lens
Claims (7)
The spectroscopic analysis device
A light source unit generating light,
A sample unit for mounting a cell containing the sample to allow light generated from the light source unit to pass through the sample;
A color filter array unit configured to arrange a plurality of color filters having a specific color, and selectively transmitting light having a specific wavelength for each color filter by passing the light passing through the sample;
And a detector configured to detect light passing through the color filter array and detect optical characteristics of the sample.
The color filter array unit is a diffuser for diffusing the light generated from the light source unit so that the light can be uniformly irradiated to the individual color filters;
An infrared cut-off lens for blocking infrared rays from light incident through the diffuser;
And a condenser lens for effectively inducing light to the individual color filters.
The light collecting lens is composed of a plurality of micro lenses corresponding to the individual color filters.
The detection unit detects light passing through the color filter array unit and converts it into an electrical signal;
An analog-to-digital converter for converting an electrical signal from the image sensor into a digital signal;
And an image signal processor for processing the converted digital signal to display characteristics of light passing through the sample.
And the image sensor is a charge-coupled device (CCD) or a complementary metal oxide semiconductor (CMOS).
Measuring the amount of light (I dark ) in a state where light is blocked;
Obtaining the measured value (I blank ) of each color filter for the reference sample;
Obtaining a measurement value (I sample ) of each color filter for the test sample by transmitting light through a test sample to be measured for density;
Comparing the amount of light attenuation for each color from the measured value (I sample ) of each color filter for the test sample;
Obtaining permeability or absorbance for the test sample;
Measuring the absorbance of the standard sample to obtain a calibration curve for absorbance and concentration;
And calculating the concentration of the test sample from the permeability or absorbance of the test sample and the calibration curve graph.
And adjusting the turbidity of the test sample by using the measured value for the color of the color filter which does not affect the color of the test sample.
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KR20100017006A KR101172012B1 (en) | 2010-02-25 | 2010-02-25 | Spectrophotometer apparatus using color filter array |
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KR20100017006A KR101172012B1 (en) | 2010-02-25 | 2010-02-25 | Spectrophotometer apparatus using color filter array |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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KR102245068B1 (en) * | 2019-10-23 | 2021-04-28 | 안양대학교 산학협력단 | Portable nano-bubble concentration measurement and analysis device |
WO2022086275A1 (en) * | 2020-10-22 | 2022-04-28 | 주식회사 테크로스 | Concentration measuring apparatus and method using absorption photometry |
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JP2006266868A (en) * | 2005-03-24 | 2006-10-05 | Toshiba Corp | Absorption analyzer and absorption analysis method |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
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KR102245068B1 (en) * | 2019-10-23 | 2021-04-28 | 안양대학교 산학협력단 | Portable nano-bubble concentration measurement and analysis device |
WO2022086275A1 (en) * | 2020-10-22 | 2022-04-28 | 주식회사 테크로스 | Concentration measuring apparatus and method using absorption photometry |
KR20220053355A (en) * | 2020-10-22 | 2022-04-29 | 주식회사 테크로스 | Apparatus and method for measuring concentration using absorption photometry |
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