JP2013138635A - Green tea extract - Google Patents

Green tea extract Download PDF

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JP2013138635A
JP2013138635A JP2011289997A JP2011289997A JP2013138635A JP 2013138635 A JP2013138635 A JP 2013138635A JP 2011289997 A JP2011289997 A JP 2011289997A JP 2011289997 A JP2011289997 A JP 2011289997A JP 2013138635 A JP2013138635 A JP 2013138635A
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green tea
tea extract
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Makoto Sato
佐藤  誠
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Kao Corp
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Abstract

PROBLEM TO BE SOLVED: To provide a green tea extract improved in taste with a decreased bitter taste component.SOLUTION: A green tea extract contains the following components (A), (B), and (C): (A) non-polymer catechins; (B) a component bonded to a magnesium ion and forming precipitate in an isopropyl alcohol; and (C) free sugar derived from tea, wherein the mass ratio [(B)/(A)] of the content of component (B) to the content of component (A) in terms of magnesium is 0.8×10or less, and the mass ratio [(C)/(A)] of the content of component (C) to the content of component (A) is 0.2 to 1.0.

Description

本発明は、緑茶抽出物に関する。   The present invention relates to a green tea extract.

緑茶飲料は、リフレッシュ作用やそれに含まれる非重合体カテキン類の生理機能が注目されており、プラスチックボトルや金属製の缶等に充填して時と場所を問わず直接飲用できるという利便性と、例えば非重合体カテキン類の配合量を増量して機能性を向上させることにより、需要が益々増大する傾向にある。このような容器詰茶飲料は、通常緑茶葉から得られた緑茶抽出物を配合して製造されるが、緑茶葉から得られた緑茶抽出物を多量に配合すると、苦味、渋味が強くなり過ぎて呈味が損なわれることがあった。   Green tea drinks are attracting attention for the refreshing action and the physiological functions of non-polymer catechins contained in it, and the convenience of being able to drink directly at any time and place by filling in plastic bottles or metal cans, For example, by increasing the amount of non-polymer catechins to improve functionality, demand tends to increase more and more. Such packaged tea beverages are usually manufactured by blending green tea extract obtained from green tea leaves, but when a large amount of green tea extract obtained from green tea leaves is blended, the bitterness and astringency become stronger. In some cases, the taste was impaired.

このような呈味の問題を解決すべく、例えば、特定のポリフェノール類の含有質量比と、非重合体カテキン類中の非重合体カテキン類のガレート体の割合を制御することで、苦味と後味を改善した緑茶抽出物(特許文献1)、非重合体カテキン類とカフェインとの含有質量比を制御することで、口の中に含んだときの後味に苦味やエグ味がなく、すっきりしていて飲みやすい緑茶抽出物(特許文献2)等が提案されている。   In order to solve such taste problems, for example, by controlling the content ratio of specific polyphenols and the ratio of gallate bodies of non-polymer catechins in non-polymer catechins, bitterness and aftertaste By controlling the content ratio of green tea extract (Patent Document 1), non-polymer catechins and caffeine, the aftertaste when contained in the mouth has no bitterness or taste, and is refreshing An easy-to-drink green tea extract (Patent Document 2) has been proposed.

特開2009−98号公報JP 2009-98 A 特開2011−15657号公報JP 2011-15657 A

緑茶飲料の呈味には苦味、渋味、旨味等の多くの要素があり、これらは嗜好性を決する上で重要な要素となる。前述の先行技術においては、原料緑茶抽出物を活性炭や酸性白土等の吸着剤や合成吸着剤で処理する方法や、該吸着剤処理後に、更に限外濾過する方法などの精製手段に供することにより、苦味を抑制できるものの、苦味成分とともに嗜好性の高い呈味成分も同時に除去されてしまうため、緑茶飲料としての呈味が不十分となる場合がある。そのため、嗜好性の高い呈味成分を損なうことなく、苦味成分を選択的に低減させた緑茶抽出物が求められている。
したがって、本発明の課題は、苦味成分を低減し呈味の改善された緑茶抽出物を提供することにある。
The taste of green tea beverages has many factors such as bitterness, astringency and umami, and these are important factors in determining palatability. In the above-mentioned prior art, by subjecting the raw green tea extract to a purification means such as a method of treating with an adsorbent such as activated carbon or acidic clay or a synthetic adsorbent, or a method of further ultrafiltration after the treatment with the adsorbent. Although the bitterness can be suppressed, the taste component with high palatability is also removed at the same time as the bitterness component, and the taste as a green tea beverage may be insufficient. Therefore, there is a need for a green tea extract in which bitterness components are selectively reduced without impairing taste components with high palatability.
Therefore, the subject of this invention is providing the green tea extract which reduced the bitterness component and improved the taste.

本発明者は、上記課題に鑑み種々検討した結果、緑茶抽出物に含まれる成分であって、マグネシウムイオンと結合しかつイソプロピルアルコール中で沈殿物を形成する成分が苦味に関与すること、茶由来の遊離糖が嗜好性の高い呈味を有するとの知見を得た。そして、緑茶抽出物中の非重合体カテキン類に対する前述の沈殿物形成成分の含有比率を低減し、かつ非重合体カテキン類に対する(C)茶由来の遊離糖の含有比率を高めることで、苦味を低減し呈味の改善された緑茶抽出物が得られることを見出した。   As a result of various investigations in view of the above problems, the present inventor is a component contained in a green tea extract, and a component that binds to magnesium ions and forms a precipitate in isopropyl alcohol is involved in bitterness, derived from tea It was found that the free sugar has a taste with high palatability. And by reducing the content ratio of the above-mentioned precipitate formation component with respect to the non-polymer catechins in the green tea extract and increasing the content ratio of (C) tea-derived free sugars with respect to the non-polymer catechins, It was found that a green tea extract with reduced taste and improved taste can be obtained.

すなわち、本発明は、次の成分(A)、(B)及び(C);
(A)非重合体カテキン類、
(B)マグネシウムイオンと結合しかつイソプロピルアルコール中で沈殿物を形成する成分、
(C)茶由来の遊離糖
を含み、
成分(A)の含有量と、成分(B)のマグネシウム換算量との質量比〔(B)/(A)〕が0.8×10-5以下であり、且つ、
成分(A)の含有量と、成分(C)の含有量との質量比〔(C)/(A)〕が0.2〜1である、緑茶抽出物を提供するものである。
That is, the present invention includes the following components (A), (B) and (C);
(A) non-polymer catechins,
(B) a component that combines with magnesium ions and forms a precipitate in isopropyl alcohol;
(C) including free sugar derived from tea,
The mass ratio [(B) / (A)] of the content of the component (A) and the magnesium equivalent amount of the component (B) is 0.8 × 10 −5 or less, and
A green tea extract having a mass ratio [(C) / (A)] of the content of the component (A) and the content of the component (C) of 0.2 to 1 is provided.

本発明はまた、茶抽出物に含まれる成分であって、(B)マグネシウムイオンと結合しかつイソプロピルアルコール中で沈殿物を形成する成分の含有量を低減させる、緑茶抽出物の呈味改善方法を提供するものである。   The present invention also relates to a method for improving the taste of a green tea extract, wherein the content of the component contained in the tea extract, which is combined with (B) magnesium ion and forms a precipitate in isopropyl alcohol, is reduced. Is to provide.

本発明によれば、緑茶抽出物の呈味を改善することができる。本発明の緑茶抽出物は、嗜好性の高いものであるから、飲食品の形態で非重合体カテキン類を継続して摂取することが可能である。   According to the present invention, the taste of the green tea extract can be improved. Since the green tea extract of the present invention has high palatability, it is possible to continuously take non-polymer catechins in the form of food and drink.

本発明の緑茶抽出物は、成分(A)として非重合体カテキン類を、成分(B)としてマグネシウムイオンと結合しかつイソプロピルアルコール中で沈殿物を形成する成分を、成分(C)として茶由来の遊離糖を、それぞれ含有するものであるが、成分(B)の含有量が緑茶抽出物に通常含まれる量よりも十分低減されているため、苦味を抑制することができる。また、緑茶抽出物中の(C)茶由来の遊離糖の含有比率が高められているため、呈味がより一層改善され、緑茶抽出物の嗜好性を向上させることができる。   The green tea extract of the present invention is a non-polymer catechin as component (A), a component that binds to magnesium ions as component (B) and forms a precipitate in isopropyl alcohol, and a component derived from tea as component (C). However, since the content of the component (B) is sufficiently lower than the amount normally contained in the green tea extract, bitterness can be suppressed. Moreover, since the content ratio of the free sugar derived from (C) tea in the green tea extract is increased, the taste is further improved and the palatability of the green tea extract can be improved.

ここで、本明細書において「(A)非重合体カテキン類」とは、カテキン、ガロカテキン、カテキンガレート及びガロカテキンガレート等の非エピ体カテキン類と、エピカテキン、エピガロカテキン、エピカテキンガレート及びエピガロカテキンガレート等のエピ体カテキン類を併せての総称であり、非重合体カテキン類の濃度は上記8種の合計量に基づいて定義される。
また、「(B)マグネシウムイオンと結合しかつイソプロピルアルコール中で沈殿物を形成する成分」とは、次の方法により沈殿が形成される成分をいう。すなわち、非重合体カテキン類濃度が175mg/100mLとなるように0.1mol/Lの硝酸溶液で希釈した緑茶抽出物100mLに、0.1mol/Lの塩化マグネシウム水溶液1mLを滴下し10分間攪拌混合した後、30分間静置して溶液Aを調製する。次いで、溶液A1mLをイソプロピルアルコール50gに滴下し、沈殿が形成される成分である。
更に、「(C)茶由来の遊離糖」とは、茶に含まれる単糖及び二糖であって、後掲の実施例に記載の方法により測定されるものをいい、具体的には、グルコース、ガラクトース、マンノース、フルクトース、ラクトース、スクロース、マルトース等を挙げることができる。
なお、成分(A)、(B)及び(C)の各含有量の測定は、後掲の実施例に記載の方法にしたがうものとする。
Here, in the present specification, “(A) non-polymer catechins” means non-epimeric catechins such as catechin, gallocatechin, catechin gallate and gallocatechin gallate, epicatechin, epigallocatechin, epicatechin gallate and It is a collective term for epi-catechins such as epigallocatechin gallate, and the concentration of non-polymer catechins is defined based on the total amount of the eight types.
Further, “(B) a component that binds to magnesium ions and forms a precipitate in isopropyl alcohol” refers to a component that forms a precipitate by the following method. That is, 1 mL of 0.1 mol / L magnesium chloride aqueous solution was added dropwise to 100 mL of green tea extract diluted with 0.1 mol / L nitric acid solution so that the concentration of non-polymer catechins was 175 mg / 100 mL, and the mixture was stirred for 10 minutes. After that, the solution A is prepared by allowing to stand for 30 minutes. Next, 1 mL of solution A is added dropwise to 50 g of isopropyl alcohol to form a precipitate.
Furthermore, “(C) tea-derived free sugar” refers to monosaccharides and disaccharides contained in tea, which are measured by the methods described in the examples below. Specifically, Examples thereof include glucose, galactose, mannose, fructose, lactose, sucrose, and maltose.
In addition, the measurement of each content of component (A), (B) and (C) shall follow the method as described in an Example mentioned later.

本発明の緑茶抽出物は、成分(A)の含有量と成分(B)のマグネシウム換算量との質量比〔(B)/(A)〕が0.8×10-5以下であるが、より一層の呈味改善の観点から、0.7×10-5以下、更に0.5×10-5以下、殊更に0.4×10-5以下であることが好ましい。なお、質量比〔(B)/(A)〕の下限値は特に限定されず0であってもよいが、生産効率の観点から、0.01×10-5、更に0.1×10-5が好ましい。 The green tea extract of the present invention has a mass ratio [(B) / (A)] of the content of the component (A) and the magnesium equivalent of the component (B) of 0.8 × 10 −5 or less, From the viewpoint of further improving the taste, it is preferably 0.7 × 10 −5 or less, more preferably 0.5 × 10 −5 or less, and particularly preferably 0.4 × 10 −5 or less. The mass ratio lower limit of [(B) / (A)] may be 0 is not particularly limited, from the viewpoint of production efficiency, 0.01 × 10 -5, further 0.1 × 10 - 5 is preferred.

また、本発明の緑茶抽出物は、成分(A)の含有量と、成分(C)の含有量との質量比〔(C)/(A)〕が0.2〜1.0であるが、より一層の呈味改善の観点から、0.25〜0.9、更に0.3〜0.85であることが好ましい。   The green tea extract of the present invention has a mass ratio [(C) / (A)] of the content of the component (A) and the content of the component (C) of 0.2 to 1.0. From the viewpoint of further improving the taste, it is preferably 0.25 to 0.9, more preferably 0.3 to 0.85.

本発明の緑茶抽出物は、より一層苦味を抑制して呈味を改善するために、成分(B)の含有量がより低いことが好ましい。具体的には、成分(B)の含有量は、本発明の緑茶抽出物の固形分中に3質量ppm未満、更に2質量ppm以下、更に1.5質量ppm以下、殊更に1質量ppm以下であることが好ましい。なお、固形分中の成分(B)の含有量の下限値は特に限定されず0質量ppmであってもよいが、生産効率の観点から、0.001質量ppm、更に0.01質量ppmであることが好ましい。ここで、本明細書において「固形分」とは、試料を105℃の電気恒温乾燥機で3時間乾燥して揮発物質を除いた残分をいう。   The green tea extract of the present invention preferably has a lower content of component (B) in order to further suppress bitterness and improve taste. Specifically, the content of the component (B) is less than 3 ppm by mass, further 2 ppm by mass or less, further 1.5 ppm by mass or less, particularly 1 ppm by mass or less in the solid content of the green tea extract of the present invention. It is preferable that In addition, the lower limit of the content of the component (B) in the solid content is not particularly limited and may be 0 mass ppm, but from the viewpoint of production efficiency, 0.001 mass ppm, and further 0.01 mass ppm. Preferably there is. As used herein, “solid content” refers to a residue obtained by drying a sample for 3 hours with an electric thermostatic dryer at 105 ° C. to remove volatile substances.

また、本発明の緑茶抽出物は、嗜好性をより一層高めるために、成分(C)の含有量がより高いことが好ましい。具体的には、成分(C)の含有量は、本発明の緑茶抽出物の固形分中に8〜40質量%、更に10〜38質量%、更に12〜35質量%であることが好ましい。   In addition, the green tea extract of the present invention preferably has a higher content of the component (C) in order to further enhance the palatability. Specifically, the content of the component (C) is preferably 8 to 40% by mass, further 10 to 38% by mass, and further 12 to 35% by mass in the solid content of the green tea extract of the present invention.

このような緑茶抽出物は、原料緑茶抽出物から水溶性高分子を除去して得ることができる。水溶性高分子としては、例えば、ペクチン等の多糖類、カフェインと非重合体カテキン類との複合体等を挙げることができる。原料緑茶抽出物から水溶性高分子を除去する方法としては、例えば、分画分子量が好ましくは3000ダルトン以下、より好ましくは500〜3000ダルトンの限外濾過膜に原料緑茶抽出物を通過させる方法が挙げられる。   Such a green tea extract can be obtained by removing the water-soluble polymer from the raw green tea extract. Examples of the water-soluble polymer include polysaccharides such as pectin, a complex of caffeine and non-polymer catechins, and the like. As a method for removing the water-soluble polymer from the raw green tea extract, for example, a method in which the raw green tea extract is passed through an ultrafiltration membrane having a fractional molecular weight of preferably 3000 daltons or less, more preferably 500 to 3000 daltons. Can be mentioned.

原料緑茶抽出物としては、例えば、緑茶葉から得られた緑茶抽出液を挙げることができる。使用する茶葉としては、例えば、Camellia属、例えば、C.var.sinensis(やぶきた種を含む)、C.var.assamica及びそれらの雑種から得られる茶葉から製茶された緑茶葉が挙げられる。製茶された緑茶葉としては、煎茶、番茶、碾茶、釜入り茶が挙げられ、茎茶、棒茶、芽茶等も使用することもできる。これらは単独で又は2種以上を組み合わせて使用することができる。   Examples of the raw green tea extract include a green tea extract obtained from green tea leaves. Examples of the tea leaves used include green tea leaves made from tea leaves obtained from the genus Camellia, for example, C. var. Sinensis (including Yabukita species), C. var. Assamica, and hybrids thereof. Examples of the green tea leaves that are made include sencha, bancha, bud tea, and tea with a kettle, and stem tea, stick tea, bud tea, and the like can also be used. These can be used alone or in combination of two or more.

抽出方法としては、例えば、攪拌抽出、カラム法、ドリップ抽出等の従来の方法を採用することができる。また、抽出用水にあらかじめアスコルビン酸ナトリウムなどの有機酸又は有機酸塩類を添加してもよい。このようにして得られた緑茶抽出液は、そのまま使用しても、希釈又は濃縮して使用してもよい。   As the extraction method, for example, conventional methods such as stirring extraction, column method, drip extraction and the like can be adopted. Further, an organic acid such as sodium ascorbate or an organic acid salt may be added to the extraction water in advance. The green tea extract thus obtained may be used as it is, or diluted or concentrated.

また、原料緑茶抽出物として、緑茶抽出液の代わりに、緑茶抽出液を水と有機溶媒との混合溶液に溶解又は分散させた状態で合成吸着剤に接触させ、合成吸着剤に吸着されずに回収された緑茶抽出液の溶液を使用してもよい。緑茶抽出液と、水と有機溶媒との混合溶液との割合は、緑茶抽出液の溶液中の固形分濃度が0.1〜10質量%、更に0.5〜3質量%となるように調整することが好ましい。混合溶液中の有機溶媒濃度は、5〜30質量%、更に10〜25質量%であることが好ましく、有機溶媒としては、エタノール等のアルコールが好ましい。   Also, as a raw green tea extract, instead of the green tea extract, the green tea extract is dissolved or dispersed in a mixed solution of water and an organic solvent and brought into contact with the synthetic adsorbent so that it is not adsorbed by the synthetic adsorbent. You may use the solution of the collect | recovered green tea extract. The ratio of the green tea extract and the mixed solution of water and organic solvent is adjusted so that the solid concentration in the green tea extract solution is 0.1 to 10% by mass, and further 0.5 to 3% by mass. It is preferable to do. The organic solvent concentration in the mixed solution is preferably 5 to 30% by mass, more preferably 10 to 25% by mass, and the organic solvent is preferably an alcohol such as ethanol.

合成吸着剤は、イオン交換能が1meq/g未満であり、かつ不溶性の三次元架橋構造を有するポリマーであることが好ましい。このような合成吸着剤としては、公知の方法により製造したものを使用しても、市販品を使用してもよい。市販の合成吸着剤として、例えば、アンバーライトXAD4、XAD16HP、XAD1180、XAD2000(供給元:米国ローム&ハース社)、ダイヤイオンHP20、HP21(三菱化学社製)、セパビーズSP−850、SP−825、SP−700、SP−70(三菱化学社製)、VPOC1062(Bayer社製)等のスチレン系;セパビーズSP205、SP206、SP207(三菱化学社製)等の芳香環に臭素原子を導入して吸着能を高めた置換スチレン系;ダイヤイオンHP1MG、HP2MG(三菱化学社製)等のメタクリル系;アンバーライトXAD761(ロームアンドハース社製)等のフェノール系;アンバーライトXAD7HP(ロームアンドハース社製)等のアクリル系;TOYOPEARL、HW-40C(東ソー社製)等のポリビニル系;SEPHADEX、LH−20(ファルマシア社製)等のデキストラン系を挙げることができる。中でも、合成吸着剤としては、その母体が、非重合体カテキン類と夾雑物との分離性の点から、スチレン系、メタクリル系、アクリル系、ポリビニル系であるものが好ましく、更にスチレン系であるものが好ましい。   The synthetic adsorbent is preferably a polymer having an ion exchange capacity of less than 1 meq / g and an insoluble three-dimensional crosslinked structure. As such a synthetic adsorbent, those produced by known methods may be used, or commercially available products may be used. Commercially available synthetic adsorbents include, for example, Amberlite XAD4, XAD16HP, XAD1180, XAD2000 (supplier: Rohm & Haas, USA), Diaion HP20, HP21 (Mitsubishi Chemical Corporation), Sepabeads SP-850, SP-825, Styrenics such as SP-700, SP-70 (manufactured by Mitsubishi Chemical), VPOC1062 (manufactured by Bayer); bromine atoms introduced into aromatic rings such as Sepabeads SP205, SP206, SP207 (manufactured by Mitsubishi Chemical), etc. Substituted styrene type with increased methacrylic; Methacrylic type such as Diaion HP1MG, HP2MG (Mitsubishi Chemical); Phenol type such as Amberlite XAD761 (Rohm and Haas); Amberlite XAD7HP (Rohm and Haas) Acrylic; TOYOPEARL, HW-4 Polyvinyl type such as 0C (manufactured by Tosoh Corporation); Dextran type such as SEPHADEX, LH-20 (Pharmacia) can be used. Among them, the synthetic adsorbent is preferably a styrene-based, methacrylic-based, acrylic-based, or polyvinyl-based one in terms of separability between non-polymer catechins and contaminants, and more preferably a styrene-based adsorbent. Those are preferred.

合成吸着剤との接触方法としては、原料緑茶抽出物に合成吸着剤を添加し撹拌した後、ろ過操作により処理液を回収するバッチ方式、合成吸着剤を充填したカラムに連続的に原料緑茶抽出物を通液し、通過液を回収する連続方式を採用することができる。中でも、生産効率の観点から、カラムによる連続方式が好ましい。
合成吸着剤との接触条件は適宜設定可能であるが、例えば、合成吸着剤を充填したカラムに原料緑茶抽出物を通液する条件としては、通液速度(SV)を0.5〜10[h-1]、合成吸着剤に対する通液倍数を0.5〜20[v/v]とすることが好ましい。更に、原料緑茶抽出物を通液後、合成吸着剤を水で洗浄することが好ましく、回収された洗浄液を前述の通過液と併せて用いることができる。なお、通液条件は、前述と同様の条件を採用することができる。
As a method of contact with the synthetic adsorbent, after adding the synthetic adsorbent to the raw green tea extract and stirring, batch processing to collect the treatment liquid by filtration operation, continuous extraction of raw green tea into the column filled with synthetic adsorbent A continuous system in which an object is passed and the passing liquid is recovered can be employed. Among these, from the viewpoint of production efficiency, a continuous method using a column is preferable.
The contact condition with the synthetic adsorbent can be set as appropriate. For example, as a condition for passing the raw green tea extract through the column filled with the synthetic adsorbent, the liquid passing rate (SV) is 0.5 to 10 [ h −1 ], and the passing ratio with respect to the synthetic adsorbent is preferably 0.5 to 20 [v / v]. Furthermore, it is preferable to wash the synthetic adsorbent with water after passing through the raw green tea extract, and the recovered washing liquid can be used in combination with the above-mentioned passing liquid. In addition, the conditions similar to the above can be employ | adopted for liquid feeding conditions.

限外濾過膜としては、疎水性であるものが好ましく、例えば、ポリスルホン系、セルロース系、ポリアクリロニトリル系、ポリイミド系又はポリフッ化炭化水素系の高分子膜を挙げることができる。中でも、水溶性高分子の除去効率の観点から、ポリスルホン系、セルロース系、ポリアクリロニトリル系の高分子膜が好ましい。ポリスルホン系高分子膜としては、例えば、ポリスルホン、ポリエーテルスルホン、ポリフェニルスルホン、ポリアリールスルホンが挙げられる。また、ポリフッ化炭化水素系高分子膜としては、例えば、ポリフッ化ビニリデンが挙げられる。更に、セルロース系高分子膜としては、例えば、酢酸セルロース、ニトロセルロース、再生セルロースが挙げられる。中でも、非重合体カテキン類と夾雑物との分離性の点から、ポリエーテルスルフォン、再生セルロースが好ましい。   The ultrafiltration membrane is preferably hydrophobic, and examples thereof include polysulfone-based, cellulose-based, polyacrylonitrile-based, polyimide-based, and polyfluorinated hydrocarbon-based polymer membranes. Among these, from the viewpoint of the removal efficiency of the water-soluble polymer, polysulfone-based, cellulose-based, and polyacrylonitrile-based polymer membranes are preferable. Examples of the polysulfone-based polymer membrane include polysulfone, polyethersulfone, polyphenylsulfone, and polyarylsulfone. An example of the polyfluorinated hydrocarbon polymer film is polyvinylidene fluoride. Furthermore, examples of the cellulose polymer membrane include cellulose acetate, nitrocellulose, and regenerated cellulose. Among these, polyether sulfone and regenerated cellulose are preferable from the viewpoint of separability between non-polymer catechins and impurities.

限外濾過膜の構造としては、例えば、平膜、スパイラル膜、中空糸膜が挙げられ、中でも、処理効率の観点から、スパイラル膜、中空糸膜が好ましい。
このような限外濾過膜として、例えば、PES(旭化成製)等の市販品を使用することができる。
Examples of the structure of the ultrafiltration membrane include a flat membrane, a spiral membrane, and a hollow fiber membrane. Among these, a spiral membrane and a hollow fiber membrane are preferable from the viewpoint of processing efficiency.
As such an ultrafiltration membrane, for example, a commercially available product such as PES (manufactured by Asahi Kasei) can be used.

限外濾過の条件としては、水溶性高分子除去の観点から、温度は5〜80℃、更に5〜30℃であることが好ましい。
限外濾過膜に通過させる際の原料緑茶抽出物中の固形分濃度は、使用する限外濾過膜の分画分子量により適宜選択可能であるが、通常0.1〜20質量%、好ましくは0.5〜10質量%である。
As conditions for ultrafiltration, from the viewpoint of removing the water-soluble polymer, the temperature is preferably 5 to 80 ° C, and more preferably 5 to 30 ° C.
The solid content concentration in the raw green tea extract when passing through the ultrafiltration membrane can be appropriately selected depending on the molecular weight cut off of the ultrafiltration membrane to be used, but is usually 0.1 to 20% by mass, preferably 0. 5 to 10% by mass.

このようにして得られた本発明の緑茶抽出物は、そのまま使用しても、濃縮又は乾燥により高濃度化してもよい。高濃度化方法としては、例えば、減圧濃縮、逆浸透膜濃縮、噴霧乾燥、凍結乾燥等が挙げられる。本発明の緑茶抽出物の形態としては、液体、スラリー、固体等の種々のものあり、適宜選択することができる。   The green tea extract of the present invention thus obtained may be used as it is, or may be concentrated by concentration or drying. Examples of the concentration increasing method include vacuum concentration, reverse osmosis membrane concentration, spray drying, freeze drying, and the like. There are various forms of the green tea extract of the present invention such as liquid, slurry, and solid, and can be appropriately selected.

本発明の緑茶抽出物は、呈味が改善されているため、例えば、飲食品の原料として使用することができるが、とりわけ飲料が好ましい。
飲食品中の緑茶抽出物の含有量は、その種類により適宜選択することが可能であるが、通常0.1〜20質量%、好ましくは0.1〜10質量%である。
Since the taste of the green tea extract of the present invention is improved, it can be used as a raw material for food and drink, for example, but a beverage is particularly preferable.
Although content of the green tea extract in food-drinks can be suitably selected according to the kind, it is 0.1-20 mass% normally, Preferably it is 0.1-10 mass%.

飲料としては、茶飲料でも、非茶系飲料でもよい。茶飲料としては、例えば、緑茶飲料、烏龍茶飲料、紅茶飲料が例示される。また、非茶系飲料としては、例えば、果汁ジュース、野菜ジュース、スポーツ飲料、アイソトニック飲料、エンハンスドウォーター、ボトルドウォーター、ニアウォーター、コーヒー飲料、栄養ドリンク剤、美容ドリンク剤等の非アルコール飲料、ビール、ワイン、清酒、梅酒、発泡酒、ウィスキー、ブランデー、焼酎、ラム、ジン、リキュール類等のアルコール飲料を挙げることができる。
また、食品としては、例えば、菓子(例えば、パン、ケーキ、クッキー、ビスケット等の焼菓子、チューインガム、チョコレート、キャンデー)、デザート(例えば、ゼリー、ヨーグルト、アイスクリーム)、レトルト食品、調味料(例えば、ソース、スープ、ドレッシング、マヨネーズ、クリーム)が挙げられる。なお、飲食品の形態は特に限定されず、摂取しやすい形態であれば、固形、粉末、液体、ゲル状、スラリー状等のいずれであってもよい。
The beverage may be a tea beverage or a non-tea beverage. Examples of tea beverages include green tea beverages, oolong tea beverages, and black tea beverages. Non-tea beverages include, for example, non-alcoholic beverages such as fruit juice, vegetable juice, sports beverage, isotonic beverage, enhanced water, bottled water, near water, coffee beverage, nutritional drink, beauty drink, beer And alcoholic beverages such as wine, sake, plum wine, happoshu, whiskey, brandy, shochu, rum, gin, and liqueurs.
Examples of the food include confectionery (for example, baked confectionery such as bread, cake, cookies, biscuits, chewing gum, chocolate, candy), dessert (for example, jelly, yogurt, ice cream), retort food, seasoning (for example, , Sauces, soups, dressings, mayonnaise, creams). In addition, the form of food / beverage products is not specifically limited, As long as it is a form which is easy to ingest, any of solid, powder, liquid, gel form, slurry form, etc. may be sufficient.

また、飲料には、酸化防止剤、香料、有機酸類、有機酸塩類、無機酸類、無機酸塩類、無機塩類、色素類、乳化剤、保存料、調味料、甘味料、酸味料、ガム、油、ビタミン、アミノ酸、果汁エキス類、野菜エキス類、花蜜エキス類、pH調整剤、品質安定剤等の添加剤を単独で又は併用して配合してもよい。なお、添加剤の配合量は、本発明の目的を阻却しない範囲内で適宜選択可能である。   In addition, beverages include antioxidants, fragrances, organic acids, organic acid salts, inorganic acids, inorganic acid salts, inorganic salts, pigments, emulsifiers, preservatives, seasonings, sweeteners, acidulants, gums, oils, Additives such as vitamins, amino acids, fruit juice extracts, vegetable extracts, nectar extracts, pH adjusters and quality stabilizers may be used alone or in combination. In addition, the compounding quantity of an additive can be suitably selected within the range which does not obstruct the objective of this invention.

飲料のpH(20℃)は、通常2〜7、好ましくは2〜6.5とすることが、呈味及び非重合体カテキン類の安定性の点で好ましい。   The pH (20 ° C.) of the beverage is usually 2 to 7, preferably 2 to 6.5, from the viewpoints of taste and stability of non-polymer catechins.

また、飲料は、ポリエチレンテレフタレートを主成分とする成形容器(いわゆるPETボトル)、金属缶、金属箔やプラスチックフィルムと複合された紙容器、瓶等の通常の包装容器に充填した容器詰飲料として提供することができる。
また、容器詰飲料は、例えば、金属缶のような容器に充填後、加熱殺菌できる場合にあっては適用されるべき法規(日本にあっては食品衛生法)に定められた殺菌条件で製造できる。PETボトル、紙容器のようにレトルト殺菌できないものについては、あらかじめ上記と同等の殺菌条件、例えばプレート式熱交換器などで高温短時間殺菌後、一定の温度迄冷却して容器に充填する等の方法が採用できる。また無菌下で、充填された容器に別の成分を配合して充填してもよい。
In addition, beverages are provided as container-packed beverages filled in ordinary packaging containers such as molded containers (so-called PET bottles), metal cans, paper containers combined with metal foil and plastic films, bottles, etc., mainly composed of polyethylene terephthalate. can do.
In addition, packaged beverages are manufactured under the sterilization conditions stipulated in the applicable regulations (Food Sanitation Law in Japan) if they can be sterilized by heating after filling in containers such as metal cans. it can. For PET bottles and paper containers that cannot be sterilized by retort, sterilize under the same conditions as above, for example, after sterilizing at high temperature and short time with a plate heat exchanger, etc. The method can be adopted. Moreover, you may mix | blend another component with the filled container under aseptic conditions.

1.非重合体カテキン類の測定
試料溶液をフィルター(0.45μm)で濾過し、高速液体クロマトグラフ(型式SCL−10AVP、島津製作所製)を用い、オクタデシル基導入液体クロマトグラフ用パックドカラム(L−カラムTM ODS、4.6mmφ×250mm:財団法人 化学物質評価研究機構製)を装着し、カラム温度35℃でグラディエント法により分析した。移動相A液は酢酸を0.1mol/L含有する蒸留水溶液、B液は酢酸を0.1mol/L含有するアセトニトリル溶液とし、試料注入量は20μL、UV検出器波長は280nmの条件で行った。なお、グラディエントの条件は、以下のとおりである。
1. Measurement of non-polymer catechins The sample solution was filtered through a filter (0.45 μm), and a packed column (L-column) for octadecyl group-introduced liquid chromatograph using a high performance liquid chromatograph (model SCL-10AVP, manufactured by Shimadzu Corporation). TM ODS, 4.6 mmφ × 250 mm (manufactured by Chemical Substance Evaluation and Research Institute) was mounted, and analysis was performed by a gradient method at a column temperature of 35 ° C. The mobile phase A solution was a distilled aqueous solution containing 0.1 mol / L of acetic acid, the B solution was an acetonitrile solution containing 0.1 mol / L of acetic acid, the sample injection amount was 20 μL, and the UV detector wavelength was 280 nm. . The gradient conditions are as follows.

時間(分) A液濃度(体積%) B液濃度(体積%)
0.0 97 3
5.0 97 3
37.0 80 20
43.0 80 20
43.5 0 100
48.5 0 100
49.0 97 3
60.0 97 3
Time (minutes) Liquid A concentration (volume%) Liquid B concentration (volume%)
0.0 97 3
5.0 97 3
37.0 80 20
43.0 80 20
43.5 0 100
48.5 0 100
49.0 97 3
60.0 97 3

2.マグネシウムイオンと結合しかつイソプロピルアルコール中で沈殿物を形成する成分のマグネシウム換算量の測定
1)試薬
塩化マグネシウム6水和物:和光純薬社製、特級、
イソプロピルアルコール:和光純薬社製、特級、
メンブレンフィルター(ミリポア社製、セルロースアセテート、0.22μm)、
硝酸:ultra pure(Agillent)
マグネシウム標準液:100mg/L、和光純薬社製、原子吸光用
2)測定装置
原子吸光光度計(Z−2000、日立製作所社製)を用いてグラファイトファーネス法にて測定した。
3)操作
非重合体カテキン類濃度が175mg/100mLとなるように0.1mol/Lの硝酸溶液で希釈した緑茶抽出物100mLに、0.1mol/Lの塩化マグネシウム水溶液1mLを滴下して10分間攪拌混合した後、30分間静置して溶液Aを調製した。次いで、溶液A1mLをイソプロピルアルコール50gに滴下して溶液Bを調製し、この溶液Bを0.22μmのメンブレンフィルターで濾過して沈殿物を取り除き、溶液Cを回収した。溶液A及び溶液C中のマグネシウム量をグラファイトファーネス法にて定量し、両測定値の差分から沈殿物のマグネシウム換算量を求めた。
4)原子吸光測定条件
キュベット:パイロチューブHR
測定波長:202.5nm スリット 0.4mm
乾燥:80−140℃(40秒)、灰化(500℃、20秒)、原子化(2200℃、5秒)
試料導入量:20μL
測定範囲:0〜100μg/L(相関係数 0.9978)にて検量線法により測定した。
2. Measurement of magnesium equivalent amount of components that bind to magnesium ions and form precipitates in isopropyl alcohol 1) Reagent Magnesium chloride hexahydrate: Wako Pure Chemical Industries, Special Grade,
Isopropyl alcohol: Wako Pure Chemical Industries, special grade
Membrane filter (Millipore, cellulose acetate, 0.22 μm),
Nitric acid: ultra pure (Agilent)
Magnesium standard solution: 100 mg / L, manufactured by Wako Pure Chemical Industries, Ltd., for atomic absorption 2) Measuring device The measurement was performed by a graphite furnace method using an atomic absorption photometer (Z-2000, manufactured by Hitachi, Ltd.).
3) Operation 1 mL of 0.1 mol / L magnesium chloride aqueous solution was added dropwise to 100 mL of green tea extract diluted with 0.1 mol / L nitric acid solution so that the non-polymer catechin concentration was 175 mg / 100 mL for 10 minutes. After stirring and mixing, the solution A was prepared by allowing to stand for 30 minutes. Next, 1 mL of the solution A was added dropwise to 50 g of isopropyl alcohol to prepare a solution B. The solution B was filtered through a 0.22 μm membrane filter to remove precipitates, and the solution C was recovered. The amount of magnesium in solution A and solution C was quantified by the graphite furnace method, and the magnesium equivalent amount of the precipitate was determined from the difference between the two measured values.
4) Atomic absorption measurement conditions Cuvette: Pyrotube HR
Measurement wavelength: 202.5 nm, slit 0.4 mm
Drying: 80-140 ° C. (40 seconds), ashing (500 ° C., 20 seconds), atomization (2200 ° C., 5 seconds)
Sample introduction amount: 20 μL
Measurement range: 0 to 100 μg / L (correlation coefficient: 0.9978).

3.茶由来の遊離糖の測定
1)試料の調製
試料5mLを、シリカベースの充填剤(BONDELUTE C18、バリアン社製)0.5g、強酸性カチオン交換樹脂(SAX、バリアン社製)0.5g、強酸性アニオン交換樹脂(SCX、バリアン社製)0.5gに順次通液して試料を調製した。
2)測定装置
高速液体クロマトグラフ(D−2000、日立製作所社製)を使用した。
3)測定条件
カラム:ULTRON PS−80N、新和化学社製
カラム温度:60℃
移動相 :純水
流量 :1mL/min
検出器 :RI
試料導入量:0.1mL
4)解析
5.3分から9分までのピーク面積をグルコース1mg/mLの面積を指標にして測定した。
3. Measurement of free sugar derived from tea 1) Preparation of sample 5 mL of sample, 0.5 g of silica-based filler (BONDELUTE C18, manufactured by Varian), 0.5 g of strongly acidic cation exchange resin (SAX, manufactured by Varian), strong acid A sample was prepared by sequentially passing through 0.5 g of anionic anion exchange resin (SCX, manufactured by Varian).
2) Measuring apparatus A high performance liquid chromatograph (D-2000, manufactured by Hitachi, Ltd.) was used.
3) Measurement conditions Column: ULTRON PS-80N, manufactured by Shinwa Chemical Co., Ltd. Column temperature: 60 ° C
Mobile phase: Pure water Flow rate: 1 mL / min
Detector: RI
Sample introduction amount: 0.1 mL
4) Analysis The peak area from 5.3 minutes to 9 minutes was measured using the area of glucose 1 mg / mL as an index.

4.官能評価
各緑茶抽出物を、非重合体カテキン類濃度が0.175g/100mLとなるようにイオン交換水で希釈して飲料を調製し、得られた飲料の呈味について、専門パネラー4名による飲用試験を行った。飲料試験は、下記の基準にしたがって評価し、その後協議により評点を決定した。
4). Sensory evaluation Each green tea extract was diluted with ion-exchanged water so that the non-polymer catechin concentration would be 0.175 g / 100 mL to prepare a beverage, and the taste of the obtained beverage was determined by four specialist panelists. A drinking test was conducted. The beverage test was evaluated according to the following criteria, and then the score was determined through consultation.

(評価基準)
評点5:苦味がなく良好である。
4:僅かに苦味があるが良好である。
3:やや苦味がある。
2:苦味がある。
1:苦味が強い。
(Evaluation criteria)
Score 5: Good without bitterness.
4: Slightly bitter but good.
3: Slightly bitter.
2: There is a bitter taste.
1: Strong bitterness.

比較例1
緑茶葉(ケニア産、大葉種)3kgに88℃の熱水45kgを添加し、60分間攪拌してバッチ抽出した。次いで、100メッシュ金網で粗ろ過後、抽出液中の微粉を除去する為に遠心分離操作を行い、緑茶抽出液36.8kg(pH5.3)を得た。緑茶抽出液中の非重合体カテキン類濃度は0.54質量%であった。また、緑茶抽出液中の固形分量は1.69質量%であった。得られた緑茶抽出液の物性を表1に示す。
Comparative Example 1
45 kg of hot water of 88 ° C. was added to 3 kg of green tea leaves (produced from Kenya, large leaf type), and batch extraction was performed by stirring for 60 minutes. Next, after coarse filtration with a 100 mesh wire netting, a centrifugal separation operation was performed to remove fine powder in the extract to obtain 36.8 kg of green tea extract (pH 5.3). The concentration of non-polymer catechins in the green tea extract was 0.54% by mass. The solid content in the green tea extract was 1.69% by mass. Table 1 shows the physical properties of the obtained green tea extract.

比較例2
市販の緑茶抽出物(ポリフェノン、三井農林製)9gをイオン交換水で希釈し、固形分濃度3質量%の緑茶抽出物水溶液300mLを得た。次に、得られた緑茶抽出物水溶液200mLを円筒状のカラムに充填した合成吸着剤(ダイヤイオンSP70、三菱化学社製)50mLにSV=1(h-1)の条件で通液した。尚、緑茶抽出物水溶液のカラム通液に先立ち、合成吸着剤の前処理を実施した。具体的には合成吸着剤に92質量%エタノール水溶液100mLをSV=1(h-1)の条件で通液し、その後、イオン交換水1LをSV=1(h-1)の条件で通液して合成吸着剤の前処理をした。
緑茶抽出物水溶液200mLをカラム通液後、次に純水75gをSV=1(h-1)の条件で通液して合成吸着剤を洗浄した。次に、カラムに吸着した成分を脱離させる為に、30質量%エタノール水溶液100mLをSV=1(h-1)の条件で通液して脱離液を得た。次に、得られた脱離液を活性炭(SGP、フタムラ社製)3.2gを充填したカラムにSV=1(h-1)の条件で全量通液し、通液処理を終えた後のエタノール水溶液を凍結乾燥して緑茶抽出物を得た。得られた緑茶抽出物の物性を表1に示す。
Comparative Example 2
9 g of a commercially available green tea extract (polyphenone, manufactured by Mitsui Norin) was diluted with ion-exchanged water to obtain 300 mL of a green tea extract aqueous solution having a solid content concentration of 3% by mass. Next, 200 mL of the obtained green tea extract aqueous solution was passed through 50 mL of a synthetic adsorbent (Diaion SP70, manufactured by Mitsubishi Chemical Corporation) packed in a cylindrical column under the condition of SV = 1 (h −1 ). Prior to passing the green tea extract aqueous solution through the column, pretreatment of the synthetic adsorbent was performed. Specifically, 100 mL of 92 mass% ethanol aqueous solution was passed through the synthetic adsorbent under the condition of SV = 1 (h −1 ), and then 1 L of ion-exchanged water was passed under the condition of SV = 1 (h −1 ). Then, the synthetic adsorbent was pretreated.
After 200 mL of the green tea extract aqueous solution was passed through the column, 75 g of pure water was then passed under conditions of SV = 1 (h −1 ) to wash the synthetic adsorbent. Next, in order to desorb components adsorbed on the column, 100 mL of a 30% by mass aqueous ethanol solution was passed under conditions of SV = 1 (h −1 ) to obtain a desorbed solution. Next, the entire amount of the obtained detachment liquid was passed through a column packed with activated carbon (SGP, manufactured by Phutamura Co., Ltd.) under the condition of SV = 1 (h −1 ), and the liquid passage treatment was completed. The aqueous ethanol solution was freeze-dried to obtain a green tea extract. Table 1 shows the physical properties of the obtained green tea extract.

比較例3
市販の緑茶抽出物(ポリフェノン、三井農林製)9gを15質量%エタノール水溶液で希釈し、固形分濃度3質量%の緑茶抽出物含有エタノール水溶液300mLを得た。次に、緑茶抽出物含有エタノール水溶液200mLを円筒状のカラムに充填した合成吸着剤(ダイヤイオンSP70、三菱化学社製)50mLにSV=1(h-1)の条件で通液し、さらに残渣を回収する為に、純水50mLをSV=1(h-1)の条件で通液して、通過液240mLを得た。この通過液を凍結乾燥して緑茶抽出物を得た。得られた緑茶抽出物の物性を表1に示す。尚、緑茶抽出物含有エタノール水溶液のカラム通液に先立ち、合成吸着剤の前処理を実施した。具体的には合成吸着剤に92質量%エタノール水溶液100mLをSV=1(h-1)の条件で通液し、その後、イオン交換水1LをSV=1(h-1)の条件で通液し、さらに15質量%エタノール水溶液100mlをSV=1(h-1)の条件で通液し合成吸着剤の前処理を実施した。
Comparative Example 3
9 g of a commercially available green tea extract (polyphenone, manufactured by Mitsui Norin) was diluted with a 15% by mass aqueous ethanol solution to obtain 300 mL of a green tea extract-containing aqueous ethanol solution having a solid content concentration of 3% by mass. Next, 200 mL of a green tea extract-containing aqueous ethanol solution was passed through 50 mL of a synthetic adsorbent (Diaion SP70, manufactured by Mitsubishi Chemical Corporation) packed in a cylindrical column under the condition of SV = 1 (h −1 ), and the residue In order to collect water, 50 mL of pure water was passed under conditions of SV = 1 (h −1 ) to obtain 240 mL of a passing solution. This passing liquid was freeze-dried to obtain a green tea extract. Table 1 shows the physical properties of the obtained green tea extract. Prior to passing the green tea extract-containing aqueous ethanol solution through the column, pretreatment of the synthetic adsorbent was performed. Specifically, 100 mL of 92 mass% ethanol aqueous solution was passed through the synthetic adsorbent under the condition of SV = 1 (h −1 ), and then 1 L of ion-exchanged water was passed under the condition of SV = 1 (h −1 ). Further, 100 ml of a 15% by mass aqueous ethanol solution was passed under conditions of SV = 1 (h −1 ) to pretreat the synthetic adsorbent.

実施例1
比較例1で得られた緑茶抽出液50mLを限外濾過膜(材質:ポリエーテルスルフォン、分画分子量500ダルトン、商品名:アミコンYC05、ミリポア社製)を用いて、濾過条件を平膜加圧濾過、液温25℃、ゲージ圧5Kg/cm2として加圧濾過し、濾過液25mLを回収した。その後、この濾過液を凍結乾燥した。得られた緑茶抽出物の物性を表1に示す。
Example 1
Using a green tea extract (50 mL) obtained in Comparative Example 1 with an ultrafiltration membrane (material: polyethersulfone, molecular weight cut off: 500 Dalton, trade name: Amicon YC05, manufactured by Millipore), the filtration conditions were flat membrane pressurization. Filtration, pressure filtration was performed at a liquid temperature of 25 ° C. and a gauge pressure of 5 kg / cm 2 , and 25 mL of the filtrate was recovered. The filtrate was then lyophilized. Table 1 shows the physical properties of the obtained green tea extract.

実施例2
比較例3で得られた緑茶抽出物1gを15質量%エタノール水溶液で希釈し、緑茶抽出物含有エタノール水溶液100mLを得た。次に、この緑茶抽出物含有エタノール水溶液50mLを限外濾過膜(材質:再生セルロース、分画分子量3000ダルトン、商品名:ウルトラセルPLBC、ミリポア社製)を用いて、濾過条件を平膜加圧濾過、液温25℃、ゲージ圧5Kg/cm2として加圧濾過し、濾過液25mLを回収した。その後、この濾過液を凍結乾燥した。得られた緑茶抽出物の物性を表1に示す。
Example 2
1 g of the green tea extract obtained in Comparative Example 3 was diluted with a 15% by mass aqueous ethanol solution to obtain 100 mL of an aqueous ethanol solution containing green tea extract. Next, 50 mL of this green tea extract-containing aqueous ethanol solution was subjected to flat membrane pressure using an ultrafiltration membrane (material: regenerated cellulose, fractional molecular weight of 3000 daltons, trade name: Ultracell PLBC, manufactured by Millipore). Filtration, pressure filtration was performed at a liquid temperature of 25 ° C. and a gauge pressure of 5 kg / cm 2 , and 25 mL of the filtrate was recovered. The filtrate was then lyophilized. Table 1 shows the physical properties of the obtained green tea extract.

Figure 2013138635
Figure 2013138635

表1から、(B)緑茶抽出物中の非重合体カテキン類に対するマグネシウムイオンと結合しかつイソプロピルアルコール中で沈殿物を形成する成分の含有割合を低減し、緑茶抽出物中の非重合体カテキン類に対する(C)茶由来の遊離糖の含有割合を高めることで、苦味を低減し呈味の改善された緑茶抽出物が得られることが確認された。   From Table 1, (B) the non-polymer catechin in the green tea extract is reduced by reducing the content of components that bind to magnesium ions and form precipitates in isopropyl alcohol with respect to the non-polymer catechins in the green tea extract. It was confirmed that a green tea extract with reduced bitterness and improved taste can be obtained by increasing the content ratio of (C) tea-derived free sugar relative to the fruit.

Claims (4)

次の成分(A)、(B)及び(C);
(A)非重合体カテキン類、
(B)マグネシウムイオンと結合しかつイソプロピルアルコール中で沈殿物を形成する成分、
(C)茶由来の遊離糖
を含み、
成分(A)の含有量と、成分(B)のマグネシウム換算量との質量比〔(B)/(A)〕が0.8×10-5以下であり、且つ、
成分(A)の含有量と、成分(C)の含有量との質量比〔(C)/(A)〕が0.2〜1.0である、緑茶抽出物。
The following components (A), (B) and (C);
(A) non-polymer catechins,
(B) a component that combines with magnesium ions and forms a precipitate in isopropyl alcohol;
(C) including free sugar derived from tea,
The mass ratio [(B) / (A)] of the content of the component (A) and the magnesium equivalent amount of the component (B) is 0.8 × 10 −5 or less, and
The green tea extract whose mass ratio [(C) / (A)] of content of a component (A) and content of a component (C) is 0.2-1.0.
固形分中の成分(B)のマグネシウム換算量が3質量ppm未満である、請求項1記載の緑茶抽出物。   The green tea extract of Claim 1 whose magnesium conversion amount of the component (B) in solid content is less than 3 mass ppm. 固形分中の成分(C)の含有量が8〜40質量%である、請求項1又は2記載の緑茶抽出物。   The green tea extract of Claim 1 or 2 whose content of the component (C) in solid content is 8-40 mass%. 茶抽出物に含まれる成分であって、(B)マグネシウムイオンと結合しかつイソプロピルアルコール中で沈殿物を形成する成分の含有量を低減させる、緑茶抽出物の呈味改善方法。
A method for improving the taste of a green tea extract, wherein the content of a component which is contained in a tea extract and which binds to magnesium ions and forms a precipitate in isopropyl alcohol is reduced.
JP2011289997A 2011-12-28 2011-12-28 Green tea extract Pending JP2013138635A (en)

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