JP2013113820A - Fine object collection tool - Google Patents
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- JP2013113820A JP2013113820A JP2011263138A JP2011263138A JP2013113820A JP 2013113820 A JP2013113820 A JP 2013113820A JP 2011263138 A JP2011263138 A JP 2011263138A JP 2011263138 A JP2011263138 A JP 2011263138A JP 2013113820 A JP2013113820 A JP 2013113820A
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- 239000002390 adhesive tape Substances 0.000 claims abstract description 51
- 239000000853 adhesive Substances 0.000 claims abstract description 34
- 230000001070 adhesive effect Effects 0.000 claims abstract description 34
- 230000001681 protective effect Effects 0.000 claims abstract description 18
- 210000004027 cell Anatomy 0.000 claims description 20
- 239000000835 fiber Substances 0.000 claims description 11
- 238000011109 contamination Methods 0.000 claims description 5
- 229920003023 plastic Polymers 0.000 claims description 5
- 239000004033 plastic Substances 0.000 claims description 5
- 235000000177 Indigofera tinctoria Nutrition 0.000 claims description 4
- 206010039101 Rhinorrhoea Diseases 0.000 claims description 4
- 239000008280 blood Substances 0.000 claims description 4
- 210000004369 blood Anatomy 0.000 claims description 4
- 210000004209 hair Anatomy 0.000 claims description 4
- 229940097275 indigo Drugs 0.000 claims description 4
- COHYTHOBJLSHDF-UHFFFAOYSA-N indigo powder Natural products N1C2=CC=CC=C2C(=O)C1=C1C(=O)C2=CC=CC=C2N1 COHYTHOBJLSHDF-UHFFFAOYSA-N 0.000 claims description 4
- 208000010753 nasal discharge Diseases 0.000 claims description 4
- 210000000582 semen Anatomy 0.000 claims description 4
- 210000004927 skin cell Anatomy 0.000 claims description 4
- 239000011248 coating agent Substances 0.000 claims description 3
- 238000000576 coating method Methods 0.000 claims description 3
- 239000012634 fragment Substances 0.000 claims description 3
- 239000002184 metal Substances 0.000 claims description 3
- 238000003860 storage Methods 0.000 abstract description 5
- 230000007774 longterm Effects 0.000 abstract 1
- 239000000463 material Substances 0.000 description 37
- 238000000034 method Methods 0.000 description 8
- 238000005070 sampling Methods 0.000 description 7
- 229920000298 Cellophane Polymers 0.000 description 4
- 229920000742 Cotton Polymers 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 238000013480 data collection Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000004820 Pressure-sensitive adhesive Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 210000005260 human cell Anatomy 0.000 description 2
- 229920002635 polyurethane Polymers 0.000 description 2
- 239000004814 polyurethane Substances 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 238000012795 verification Methods 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
Abstract
Description
本発明は、微物、特に犯罪現場などに遺留された微物を採取・搬送・保存するための用具に関するものである。 The present invention relates to a tool for collecting, transporting, and storing fine objects, particularly fine objects left in crime scenes.
近年、ＤＮＡ型鑑定の高度化に伴って、犯罪鑑識におけるＤＮＡ型鑑定の重要性が増している。また、ＤＮＡ検出感度の向上に伴って，微細な資料（接触資料）からの鑑定が急増している。
また、該資料の取り違え防止のために、立証措置の徹底、資料採取から搬送、保管、鑑定着手までの期間の厳密な管理が求められている。さらに、再鑑定に対する配慮から、鑑定残余の保存と適切な管理が義務づけられている。
In recent years, with the advancement of DNA type identification, the importance of DNA type identification in crime identification has increased. In addition, with the improvement of DNA detection sensitivity, appraisal from minute materials (contact materials) has increased rapidly.
In addition, in order to prevent the mix-up of the materials, thorough verification measures, and strict management of the period from the collection of the materials to transportation, storage, and the operator of fixing the documents are required. Furthermore, preservation of appraisal residue and appropriate management are obligatory for consideration of re-appraisal.
従来、ＤＮＡ型鑑定のための資料採取は、蒸留水で湿らせた綿棒やガーゼ片、市販の粘着テープやリタックシートを用いて行われてきた。また、ポリウレタンゲルシートの粘着性を利用した微物採取用具を用いた方法も既に知られている（参照：特許文献１）。 Conventionally, collection of data for DNA type identification has been performed using a cotton swab or gauze piece moistened with distilled water, a commercially available adhesive tape, or a tack sheet. In addition, a method using a tool for collecting fine materials using the adhesiveness of a polyurethane gel sheet is already known (see Patent Document 1).
蒸留水で湿らせた綿棒やガーゼ片による資料採取では、ＤＮＡ型鑑定に必要な細胞片は水に溶けないために、採取効率は低い。さらに、該資料採取では、乾燥した状態では粘着性がないため微量の資料を採取することは困難であった。
加えて、該資料採取では、水で湿った状態では細菌によるＤＮＡの分解が進行するため早期の乾燥が必要であり、乾燥時に異物が混入する可能性や資料を取り違える危険性があった。なお、採取された細胞数を確認することができないために、ＤＮＡ型鑑定の成否を判断することは困難であり、微量な資料を再鑑定用に残すことは不可能であった。
When collecting data with a cotton swab or gauze piece moistened with distilled water, the collection efficiency is low because cell pieces necessary for DNA type identification do not dissolve in water. Further, in collecting the data, it was difficult to collect a small amount of data because there was no stickiness in the dry state.
In addition, when collecting the data, DNA is degraded by bacteria when it is wet with water, so that early drying is necessary, and there is a possibility that foreign materials may be mixed during drying and there is a risk of mistaking the data. Since the number of collected cells could not be confirmed, it was difficult to determine the success or failure of DNA type identification, and it was impossible to leave a small amount of data for re-examination.
市販の粘着テープやリタックシートによる資料採取では、採取者が資料採取時に粘着面の一部を掴む必要があり、該採取者の細胞片が混入する可能性がある。また、資料採取後の粘着面を保護するため清浄なポリ袋などに収納する必要がある。しかし、粘着力があるため取り扱いが難しく、異物が付着しないように細心の注意が必要であった。さらに、粘着力が強い場合、一度採取された細胞を剥がすことは困難であり、粘着面の一部を切り取って鑑定する必要があり、該粘着面のみならず非粘着面にも異物が付着する危険性があった。 When collecting data using a commercially available adhesive tape or retuck sheet, it is necessary for the collector to grab a part of the adhesive surface when collecting the data, and there is a possibility that the cell pieces of the collector are mixed. Moreover, it is necessary to store in a clean plastic bag etc. in order to protect the adhesive surface after data collection. However, it is difficult to handle due to the adhesive force, and it is necessary to pay close attention to prevent foreign matter from adhering. In addition, when the adhesive strength is strong, it is difficult to peel off the cells once collected, and it is necessary to cut out a part of the adhesive surface for identification, and foreign matter adheres not only to the adhesive surface but also to the non-adhesive surface. There was a danger.
ポリウレタンゲルシートの粘着性を利用した微物採取用具による資料採取では、粘着力が比較的弱いために、陳旧で強固に付着した細胞片を採取することは困難である。
また、鑑定の際は、一度採取された微物を綿棒などで拭き取る操作が必要であり、操作が煩雑であると共に資料取り違えの危険性が生じる。さらに、微物採取用具に捕集された全ての細胞片をＤＮＡ型鑑定に利用することは不可能であり、資料の残量を確認し、保管することも困難であった．
It is difficult to collect old and firmly attached cell pieces when collecting data with a tool for collecting fine materials using the adhesiveness of a polyurethane gel sheet because of its relatively weak adhesive strength.
Further, when appraisal is performed, an operation of wiping fines once collected with a cotton swab or the like is necessary, and the operation is complicated and there is a risk of mistaking materials. Furthermore, it was impossible to use all the cell pieces collected in the tool for collecting fine materials for DNA type identification, and it was difficult to check and store the remaining amount of data.
本発明の課題は、上記資料採取の問題点を解決した微物採取用具を提供することである。より詳しくは、犯罪現場などに残存する微物、特にＤＮＡ型鑑定に必要な微細な細胞片を効率良く採取すると共に、他人の細胞が混入する危険性がなく、資料の取り違えが起こり難く、さらに資料残余の適正な管理と長期保存が可能な微物採取用具を提供することである。 An object of the present invention is to provide a tool for collecting fine objects that solves the above-mentioned problem of collecting data. More specifically, fine materials remaining in crime scenes, especially fine cell fragments necessary for DNA type identification, are efficiently collected, and there is no risk of contamination by other people's cells. The aim is to provide a tool for collecting fine materials that can be properly managed and stored for a long time.
本発明者らは、「台紙と、該台紙の一部又は全部に着脱自在に設けられた剥離シートと、粘着面の両端に支持部を付けた透明粘着テープと、ここで、該透明粘着テープの粘着面の一部が該剥離シートに貼着されており、該透明粘着テープの非粘着面の一部又は全部を覆う透明保護シートと、を含む微物採取用具」が上記課題を解決できることを確認して、本発明を完成するに至った。 The present inventors have described, “Mount, a release sheet that is detachably provided on a part or all of the mount, a transparent adhesive tape with support portions on both ends of the adhesive surface, and the transparent adhesive tape,” A part of the pressure-sensitive adhesive surface of the transparent adhesive tape is attached to the release sheet, and a transparent protective sheet covering a part or all of the non-adhesive surface of the transparent adhesive tape, As a result, the present invention was completed.
すなわち、本発明は以下の通りである。
「１．微物採取用具であって、
台紙と、
該台紙の一部又は全部に着脱自在に設けられた剥離シートと、
粘着面の両端に支持部を付けた透明粘着テープと、ここで、該透明粘着テープの粘着面の一部が該剥離シートに貼着されており、
該透明粘着テープの非粘着面の一部又は全部を覆う透明保護シートと、を含み、
微物採取時において該剥離シートの存在により台紙の汚染防止が可能となり、並びに、 微物採取後において該剥離シートを剥がして、該微物が付着した透明粘着テープの粘着面と該台紙の貼着により異物混入を防止可能であることを特徴とする、微物採取用具。
２．前記剥離シートは前記台紙の一部のみに着脱自在に設けられており、並びに、前記支持部の一方が、該剥離シートが設けられていない台紙の部分に貼着されていることを特徴とする前項１に記載の微物採取用具。
３．前記微物が、細胞片、塗膜片、金属片、プラスチック片、及び／又は、繊維片である前項１又は２に記載の微物採取用具。
４．皮膚細胞、口腔内細胞、鼻汁斑、精液斑，白色繊維片が微物である場合には台紙の色は濃色であり、飛沫血痕、黒色短毛，黒色若しくは藍色繊維片が微物である場合には台紙の色は淡色であることを特徴とする前項１〜３のいずれか１に記載の微物採取用具。」
That is, the present invention is as follows.
“1. It is a tool for collecting fine objects,
Mount and
A release sheet detachably provided on a part or all of the mount,
A transparent adhesive tape with supporting portions at both ends of the adhesive surface, and here, a part of the adhesive surface of the transparent adhesive tape is adhered to the release sheet,
A transparent protective sheet covering a part or all of the non-adhesive surface of the transparent adhesive tape,
The presence of the release sheet at the time of collecting fines makes it possible to prevent the mount from being contaminated. A tool for collecting fine objects, characterized in that contamination by foreign matter can be prevented by wearing.
2. The release sheet is detachably provided only on a part of the mount, and one of the support portions is attached to a portion of the mount on which the release sheet is not provided. The tool for collecting fine objects according to item 1 above.
3. 3. The tool for collecting fine objects according to item 1 or 2, wherein the fine substance is a cell piece, a coating piece, a metal piece, a plastic piece, and / or a fiber piece.
4). When skin cells, oral cells, nasal discharge spots, semen spots, and white fiber pieces are fine, the color of the mount is dark, and splashed blood stains, black short hair, black or indigo fiber pieces are fine. 4. The tool for collecting fine objects according to any one of items 1 to 3, wherein the color of the mount is light in some cases. "
本発明の微物採取用具は、以下の効果を示す。
（１）支持部に一連番号や符号を記入することができるため、採取資料の特定が容易である。
（２）微物採取時において剥離シートの存在により台紙の汚染防止が可能である。
（３）微物採取後において剥離シートを剥がして、微物が付着した透明粘着テープの粘着面と台紙の貼着により異物混入を防止可能である。
（４）透明粘着テープの非粘着面の一部又は全部を覆う透明保護シートの存在により、資料が十分に採取できたかを容易に確認でき、さらには資料の残存量を容易に把握することができる。
（５）資料残余がある場合は、透明粘着テープの残り部分を台紙５０に貼着して保存することができる。
（６）採取・搬送・鑑定・保存の何れの段階でも、転写したり移し替えたりする必要がないために、資料のすり替えや取り違えの危険性を排除することが出来る。
The fines sampling tool of the present invention exhibits the following effects.
(1) Since the serial number and code can be entered on the support part, it is easy to specify the collected data.
(2) It is possible to prevent the mount from being contaminated by the presence of the release sheet when collecting fines.
(3) It is possible to prevent foreign matter from being mixed by peeling off the release sheet after collecting the fine objects, and sticking the adhesive surface of the transparent adhesive tape to which the fine objects have adhered and the mount.
(4) The presence of a transparent protective sheet covering part or all of the non-adhesive surface of the transparent adhesive tape makes it easy to confirm whether or not the material has been collected, and to easily grasp the remaining amount of the material. it can.
(5) If there is a remaining material, the remaining portion of the transparent adhesive tape can be attached to the mount 50 and stored.
(6) Since there is no need to transfer or transfer at any stage of sampling, transport, appraisal, and storage, the risk of material replacement or confusion can be eliminated.
以下、本発明を実施するための形態について説明するが、何ら請求項の範囲を限定するものではない。 Hereinafter, although the form for implementing this invention is demonstrated, the range of a claim is not limited at all.
（本発明の実施態様の微物採取用具の構成）
本発明の実施態様の微物採取用具１００は、図１に示すような形態であり、詳しくは、以下の通りである。
短手方向（縦）２ｃｍ、長手方向（横）３ｃｍの透明粘着テープ１０（例えば、セロファンテープ）の粘着面の両端に支持部２０を付けており、該支持部２０と該透明粘着テープ１０の非粘着面の一部又は全部が透明保護シート３０（例、透明アセテート製の保護シート）により覆われている。
なお、透明粘着テープ１０は、粘着力が水溶液中でも僅かにしか低下しないものが好ましく、さらに、ＤＮＡを抽出するために、ＰＣＲを阻害する物質を含まないものが好ましい。
剥離シート４０（好ましくは、透明な剥離シートであり、シリコン製である）は、着脱自在に台紙５０（例、剥離紙）の一部又は全部に設けられている。
さらに、該透明粘着テープ１０の粘着面の一部が該剥離シート４０に貼着している。
加えて、本発明の実施態様の微物採取用具の作製に際しては、ヒト細胞による汚染を回避するため、好ましくはクリーンベンチを用いて行う。
(Configuration of the tool for collecting fine objects according to the embodiment of the present invention)
A fine object collecting tool 100 according to an embodiment of the present invention has a form as shown in FIG. 1 and is described in detail below.
Support portions 20 are attached to both ends of the adhesive surface of the transparent adhesive tape 10 (for example, cellophane tape) of 2 cm in the short direction (longitudinal) and 3 cm in the longitudinal direction (horizontal), and the support portion 20 and the transparent adhesive tape 10 Part or all of the non-adhesive surface is covered with a transparent protective sheet 30 (eg, a protective sheet made of transparent acetate).
The transparent adhesive tape 10 preferably has a pressure-sensitive adhesive force that slightly decreases even in an aqueous solution, and preferably does not contain a substance that inhibits PCR in order to extract DNA.
The release sheet 40 (preferably a transparent release sheet and made of silicon) is detachably provided on part or all of the mount 50 (for example, release paper).
Furthermore, a part of the adhesive surface of the transparent adhesive tape 10 is adhered to the release sheet 40.
In addition, the production of the tool for collecting fine objects according to the embodiment of the present invention is preferably performed using a clean bench in order to avoid contamination by human cells.
（本発明の別の実施態様の微物採取用具の構成）
本発明の別の実施態様の微物採取用具１１０は、図２に示すように、支持部２０の一方（参照：図２の２０１）と、剥離シート４０を設けた台紙５０の片端を結合させた形態である。
(Configuration of the fine object sampling tool of another embodiment of the present invention)
As shown in FIG. 2, the fine object collecting tool 110 according to another embodiment of the present invention combines one end of the support portion 20 (refer to 201 in FIG. 2) and one end of the mount 50 provided with the release sheet 40. It is a form.
（本発明の実施態様の微物採取用具の使用方法）
本発明の微物採取用具１００の使用方法は、以下の手順であるが特に限定されない。
（１）図３に示すように、支持部２０の裏面（参照：図３の矢印３０１）に採取番号を記載する。
（２）透明保護シート３０に一部又は全部が覆われている透明粘着テープ１０を、剥離シート４０が設けられた台紙５０から剥がす｛参照：図４（１）｝。
（３）該透明粘着テープ１０を、資料採取箇所を覆うように密着させる｛参照：図４（２）｝。
（４）該透明粘着テープ１０を該透明保護シート３０の上から数回〜数十回強く押しつけることにより、該透明粘着テープ１０の粘着面に資料を付着させることにより、該資料を採取する｛参照：図４（３）｝。
（５）上記（４）の資料採取後は、剥離シート４０を台紙５０から剥がし｛参照：図４（４）｝、該透明粘着テープ１０を台紙５０に貼着して、該透明粘着テープ１０の粘着面を保護する｛参照：図４（５）｝。
(Method of using the tool for collecting fines according to the embodiment of the present invention)
The method for using the fine object sampling tool 100 of the present invention is the following procedure, but is not particularly limited.
(1) As shown in FIG. 3, the collection number is written on the back surface of the support portion 20 (reference: arrow 301 in FIG. 3).
(2) The transparent adhesive tape 10 partially or entirely covered with the transparent protective sheet 30 is peeled off from the mount 50 provided with the release sheet 40 {Reference: FIG. 4 (1)}.
(3) The transparent adhesive tape 10 is brought into close contact so as to cover the material collection location {Reference: FIG. 4 (2)}.
(4) Collecting the material by attaching the material to the adhesive surface of the transparent adhesive tape 10 by pressing the transparent adhesive tape 10 strongly over the transparent protective sheet 30 several times to several tens of times { Reference: FIG. 4 (3)}.
(5) After collecting the data in (4) above, the release sheet 40 is peeled off from the mount 50 {Reference: FIG. 4 (4)}, the transparent adhesive tape 10 is attached to the mount 50, and the transparent adhesive tape 10 {Reference: FIG. 4 (5)}.
（採取した資料の鑑定方法）
ＤＮＡ型鑑定では、透明保護シート３０越しに、実体顕微鏡や生物顕微鏡を用いて採取した資料を観察する。観察後において、使用する資料の量が決まったら、台紙５０から透明粘着テープ１０を剥がし、さらに該透明粘着テープ１０の非粘着面を覆っている透明保護シート３０を捲る。
加えて、必要とする資料量に合わせて透明粘着テープ１０を切り取り、さらに、直接、プラスチック製遠心チューブに入れる。次に、試薬を該チューブに加えた後に、ＤＮＡを抽出する。すなわち、本発明の微物採取用具で採取した微物を含む透明粘着テープ１０の前処理は不要である。
さらに、資料残余がある場合は、透明粘着テープ１０の残り部分を保護シート３０で再び覆い、台紙５０に貼着して保存することができる。
(Appraisal method for collected data)
In DNA type identification, materials collected using a stereomicroscope or a biological microscope are observed through the transparent protective sheet 30. After the observation, when the amount of the material to be used is determined, the transparent adhesive tape 10 is peeled off from the mount 50, and the transparent protective sheet 30 covering the non-adhesive surface of the transparent adhesive tape 10 is rolled up.
In addition, the transparent adhesive tape 10 is cut out according to the required amount of data and further directly put into a plastic centrifuge tube. Next, DNA is extracted after the reagent is added to the tube. That is, the pretreatment of the transparent adhesive tape 10 containing fines collected with the fines collection tool of the present invention is not necessary.
Furthermore, when there is a material residue, the remaining portion of the transparent adhesive tape 10 can be covered again with the protective sheet 30 and attached to the mount 50 for storage.
（本発明の別の実施態様の微物採取用具の使用方法）
本発明の微物採取用具１１０の使用方法は、以下の手順であるが特に限定されない。
（１）採取者は、台紙５０と結合していない方の支持部２０を掴んで、透明粘着テープ１０を剥離シート４０から剥がす。
（２）該透明粘着テープ１０を、資料採取箇所を覆うように密着させる。
（３）該透明粘着テープ１０を該透明保護シート３０の上から数回〜数十回強く押しつけることにより、該透明粘着テープ１０の粘着面に資料を付着させることにより、該資料を採取する。
（４）上記（３）の資料採取後は、保護シート３０で覆われた透明粘着テープ１０を、剥離シート４０を剥がした台紙５０に貼着し、該透明粘着テープ１０の粘着面を保護する。
なお、微物採取用具１１０は、台紙５０と透明粘着テープ１０が一体化しているため、資料採取時やＤＮＡ型鑑定後に資料残余が生じた場合でも台紙５０を紛失しない。
(Method of using the tool for collecting fine objects of another embodiment of the present invention)
The method of using the fine object sampling tool 110 of the present invention is the following procedure, but is not particularly limited.
(1) The collector grips the support part 20 that is not coupled to the mount 50 and peels the transparent adhesive tape 10 from the release sheet 40.
(2) The transparent adhesive tape 10 is brought into close contact so as to cover the material collection location.
(3) The material is collected by pressing the transparent adhesive tape 10 from the top of the transparent protective sheet 30 several times to several tens of times to attach the material to the adhesive surface of the transparent adhesive tape 10.
(4) After collecting the data of (3) above, the transparent adhesive tape 10 covered with the protective sheet 30 is attached to the mount 50 from which the release sheet 40 has been peeled off to protect the adhesive surface of the transparent adhesive tape 10. .
In addition, since the base material 50 and the transparent adhesive tape 10 are integrated, the fine material collection tool 110 does not lose the base material 50 even when the material residue is generated at the time of data collection or after DNA type identification.
（本発明の微物）
本発明の微物は、組織片、細胞片、塗膜片、金属片、プラスチック片、及び／又は、繊維片を対象とするが特に限定されない。より詳しくは、皮膚細胞、口腔内細胞、鼻汁斑、精液斑，白色繊維片、飛沫血痕、黒色短毛，黒色若しくは藍色繊維片を例示するが特に限定されない。
(Fine article of the present invention)
The fine article of the present invention is not particularly limited, although it is intended for tissue pieces, cell pieces, coating pieces, metal pieces, plastic pieces and / or fiber pieces. More specifically, skin cells, oral cells, nasal discharge spots, semen spots, white fiber pieces, splashed blood stains, black short hairs, black or indigo fiber pieces are exemplified, but not particularly limited.
（本発明の微物採取用具の特徴）
採取者は、本発明の微物採取用具の支持部２０を掴んで資料採取作業を行うため透明粘着テープ１０の粘着面に触れる恐れがない。
透明粘着テープ１０は、被採取箇所に圧着する際には、透明保護シート３０の上から押し付けられるために非粘着面にも触れる必要がなく、採取者の細胞が混入する危険性がない。
さらに、資料採取後は、粘着テープ１０の粘着面を、剥離シート４０を剥がした台紙５０に貼着して保存するため、異物が混入する可能性はほとんどない。
(Features of the tool for collecting fines of the present invention)
The collector does not have a risk of touching the adhesive surface of the transparent adhesive tape 10 because he / she grasps the support portion 20 of the fine object sampling tool of the present invention and performs the data collection operation.
Since the transparent adhesive tape 10 is pressed from above the transparent protective sheet 30 when it is pressure-bonded to the collection site, there is no need to touch the non-adhesive surface, and there is no risk of the sampler's cells being mixed.
Furthermore, since the adhesive surface of the adhesive tape 10 is pasted and stored on the backing sheet 50 from which the release sheet 40 has been peeled off after collecting the data, there is almost no possibility of foreign matter entering.
皮膚細胞、口腔内細胞、鼻汁斑、精液斑，白色繊維片が微物である場合には、好ましくは台紙５０の色は濃色である。また、飛沫血痕、黒色短毛，黒色若しくは藍色繊維片が微物である場合には、好ましくは台紙５０の色は淡色である。
このように採取する微物により、台紙５０の色を変えることにより、採取された微物の形状を肉眼で確認し易く立証措置も容易に行える。
さらに、 実体顕微鏡や生物顕微鏡を用いて採取資料を観察することができるため，採取された資料量を確認することができ、ＤＮＡ型鑑定に必要な細胞数を容易に推定することができる。
When skin cells, oral cells, nasal discharge spots, semen spots, and white fiber pieces are fine, preferably the color of the mount 50 is dark. Further, when the splashed blood stain, black short hair, black or indigo fiber piece is fine, the mount 50 is preferably light in color.
By changing the color of the mount 50 according to the fine material collected in this way, the shape of the collected fine material can be easily confirmed with the naked eye, and verification measures can be easily performed.
Furthermore, since the collected data can be observed using a stereomicroscope or a biological microscope, the amount of collected data can be confirmed, and the number of cells required for DNA type identification can be easily estimated.
ＤＮＡ型鑑定を行う場合は、台紙５０から剥がした透明粘着テープ１０の非粘着面を覆っている透明保護シート３０を捲って、透明粘着テープ１０の全体又は一部を切り取り、直接ＤＮＡ抽出を行うことが出来る。よって、採取した全ての細胞を鑑定に使用することが可能である。 When performing DNA type identification, the transparent protective sheet 30 covering the non-adhesive surface of the transparent adhesive tape 10 peeled off from the mount 50 is turned over, and the whole or a part of the transparent adhesive tape 10 is cut out to directly extract DNA. I can do it. Therefore, it is possible to use all the collected cells for identification.
ＤＮＡ型鑑定において、資料残余が生じた場合は、残った透明粘着テープ１０を再度台紙５０に貼着し、透明保護シート３０を戻して粘着テープの非粘着面を覆うことで、鑑定人の細胞が混入する危険性を排して長期に保存することが出来る。
さらに、本発明の微物採取用具を用いて採取された資料は、採取・搬送・鑑定・保存の何れの段階でも、転写したり移し替えたりする必要がないために、資料のすり替えや取り違えの危険性を排除することが出来る。
In the DNA type appraisal, if there is a residual material, the remaining transparent adhesive tape 10 is attached to the backing sheet 50 again, the transparent protective sheet 30 is returned and the non-adhesive surface of the adhesive tape is covered, so that the appraiser's cells It can be stored for a long time without the risk of contamination.
Furthermore, since materials collected using the tool for collecting fine objects according to the present invention do not need to be transferred or transferred at any stage of collection, transportation, appraisal, and storage, the materials can be replaced or misplaced. Risk can be eliminated.
以下、実施例を挙げて本発明を詳細に説明するが、本発明の範囲はこれらの実施例により限定されるものではない。 EXAMPLES Hereinafter, although an Example is given and this invention is demonstrated in detail, the scope of the present invention is not limited by these Examples.
（微物採取用具によるＤＮＡ検出）
使用済み長袖シャツの袖口・襟部、使用済みズックの靴ひも・中敷き、使用済み綿手袋の外側・内側、布製椅子の背もたれ、及び、清浄なガラス片に付着した指紋から、微物採取用具１００及び微物採取用具１１０を用いて細胞片を採取し、採取した全量を用いてＤＮＡを抽出した。
(DNA detection with a tool for collecting fine objects)
From the cuffs and collars of used long-sleeved shirts, used shoe laces and insoles, the outer and inner sides of used cotton gloves, the back of fabric chairs, and fingerprints attached to clean glass pieces 100 And the cell piece was extract | collected using the fine substance collection tool 110, and DNA was extracted using the collect | collected whole quantity.
上記抽出したＤＮＡ抽出液の１０分の１を用いて、市販のマルチプレックスＳＴＲ検査キットであるアイデンティファイラー（ライフテクノロジーズ社製）による型判定を行った。該判定では、すべての資料で正確な型判定結果であった。
さらに、微物採取用具１００及び微物採取用具１１０を用いて得られたＤＮＡ量を比較したところ、両者に有意な差異は認められなかった。
Using one-tenth of the extracted DNA extract, type determination was performed with an identifier filer (manufactured by Life Technologies), which is a commercially available multiplex STR test kit. In this determination, the accurate type determination results were obtained for all materials.
Furthermore, when the amount of DNA obtained using the fine sample collection tool 100 and the fine sample collection tool 110 was compared, no significant difference was found between the two.
（微物採取用具によるコントロール実験）
微物採取用具１００及び微物採取用具１１０の未使用の透明粘着テープ（セロファンテープ）全体を用いてＤＮＡが抽出されるかどうかを確認した。該抽出液の全量を用いて、市販のマルチプレックスＳＴＲ検査キットであるアイデンティファイラー（ライフテクノロジーズ社製）による型判定を行った。いずれも型判定可能なピークは得られず、ヒト細胞の付着は検出されなかった。
(Control experiment using fine tool)
It was confirmed whether or not DNA was extracted using the entire unused transparent adhesive tape (cellophane tape) of the fine collection tool 100 and the fine collection tool 110. Using the whole amount of the extract, type determination was performed by an identity filer (manufactured by Life Technologies), which is a commercially available multiplex STR inspection kit. In any case, no type-determinable peak was obtained, and adhesion of human cells was not detected.
微物採取用具１００及び微物採取用具１１０の未使用の透明粘着テープ（セロファンテープ全体）を用いて抽出した抽出液の全量を標準ＤＮＡ（１ナノグラム）と共に加えて、市販のマルチプレックスＳＴＲ検査キットであるアイデンティファイラー（ライフテクノロジーズ社製）による型判定を行い、ＤＮＡ抽出液を加えない対照と比較した。いずれの試料も、対照と同じ結果が得られた。
以上により、透明粘着テープであるセロファンテープの材質や粘着剤はＰＣＲを阻害せず、ＤＮＡ型鑑定に影響を及ぼさないことを確認した。
A commercially available multiplex STR test kit is prepared by adding the total amount of the extract extracted with the unused transparent adhesive tape (entire cellophane tape) of the fine sample collection tool 100 and the fine sample collection tool 110 together with the standard DNA (1 nanogram). The type was determined by an identity filer (manufactured by Life Technologies) and compared with a control to which no DNA extract was added. All samples gave the same results as the control.
From the above, it was confirmed that the material and adhesive of cellophane tape, which is a transparent adhesive tape, do not inhibit PCR and do not affect DNA type identification.
１０：透明粘着テープ
２０：支持部
３０：透明保護シート
４０：剥離シート
５０：台紙
６０：長手方向矢印
７０：短手方向矢印
１００：本発明の実施例の微物採取用具
１１０：本発明の別の実施例の微物採取用具
２０１：台紙と結合させた支持部
３０１：矢印
DESCRIPTION OF SYMBOLS 10: Transparent adhesive tape 20: Support part 30: Transparent protective sheet 40: Release sheet 50: Mount 60: Longitudinal arrow 70: Short direction arrow 100: Fine-collection tool of the Example of this invention 110: Another of this invention Fine sample collection tool 201: Support unit combined with mount 301: Arrow
Claims (4)
台紙と、
該台紙の一部又は全部に着脱自在に設けられた剥離シートと、
粘着面の両端に支持部を付けた透明粘着テープと、ここで、該透明粘着テープの粘着面の一部が該剥離シートに貼着されており、
該透明粘着テープの非粘着面の一部又は全部を覆う透明保護シートと、を含み、
微物採取時において該剥離シートの存在により台紙の汚染防止が可能となり、並びに、 微物採取後において該剥離シートを剥がして、該微物が付着した透明粘着テープの粘着面と該台紙の貼着により異物混入を防止可能であることを特徴とする、微物採取用具。 A tool for collecting fine objects,
Mount and
A release sheet detachably provided on a part or all of the mount,
A transparent adhesive tape with supporting portions at both ends of the adhesive surface, and here, a part of the adhesive surface of the transparent adhesive tape is adhered to the release sheet,
A transparent protective sheet covering a part or all of the non-adhesive surface of the transparent adhesive tape,
The presence of the release sheet at the time of collecting fines makes it possible to prevent the mount from being contaminated. A tool for collecting fine objects, characterized in that contamination by foreign matter can be prevented by wearing.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2016090351A (en) * | 2014-11-03 | 2016-05-23 | 株式会社エクシールコーポレーション | Micro evidence collection tool and method for collecting and appraising micro evidence |
| JP2017036987A (en) * | 2015-08-10 | 2017-02-16 | 株式会社エクシールコーポレーション | Fine object sampling tool and method for sampling and examining fine object |
| JP2019074336A (en) * | 2017-10-12 | 2019-05-16 | 株式会社エクシール | Microscopic material collecting tool |
| JP7402457B1 (en) | 2023-01-18 | 2023-12-21 | 日本ソフトウェアマネジメント株式会社 | Will sheets, appraisal sheets, will kits, and appraisal methods related to wills |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001161697A (en) * | 1999-12-03 | 2001-06-19 | Sony Cp Laboratories Inc | Adhesive tape for collecting skin epidermis |
| JP2004198397A (en) * | 2002-10-24 | 2004-07-15 | Kubota Matsushitadenko Exterior Works Ltd | Sheet for evaluating degradation of coating film, and method for evaluating degradation of the coating film using the same |
| US20050008536A1 (en) * | 2002-11-08 | 2005-01-13 | Cumorah, Inc. D/B/A The Provident Group | Method and apparatus for surface sampling |
| JP2006333890A (en) * | 2005-05-31 | 2006-12-14 | Kaijo Hoanchiyou Chokan | Fingerprinting sheet |
| JP2007181526A (en) * | 2006-01-05 | 2007-07-19 | Nitto Denko Corp | Adhesive sheet for identification, its manufacturing method and trace sampling method |
| JP2011117915A (en) * | 2009-12-07 | 2011-06-16 | Ekushiiru Corporation:Kk | Fine object sampling tool and fine object sampling method |
-
2011
- 2011-11-30 JP JP2011263138A patent/JP5859295B2/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001161697A (en) * | 1999-12-03 | 2001-06-19 | Sony Cp Laboratories Inc | Adhesive tape for collecting skin epidermis |
| JP2004198397A (en) * | 2002-10-24 | 2004-07-15 | Kubota Matsushitadenko Exterior Works Ltd | Sheet for evaluating degradation of coating film, and method for evaluating degradation of the coating film using the same |
| US20050008536A1 (en) * | 2002-11-08 | 2005-01-13 | Cumorah, Inc. D/B/A The Provident Group | Method and apparatus for surface sampling |
| JP2006333890A (en) * | 2005-05-31 | 2006-12-14 | Kaijo Hoanchiyou Chokan | Fingerprinting sheet |
| JP2007181526A (en) * | 2006-01-05 | 2007-07-19 | Nitto Denko Corp | Adhesive sheet for identification, its manufacturing method and trace sampling method |
| JP2011117915A (en) * | 2009-12-07 | 2011-06-16 | Ekushiiru Corporation:Kk | Fine object sampling tool and fine object sampling method |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2016090351A (en) * | 2014-11-03 | 2016-05-23 | 株式会社エクシールコーポレーション | Micro evidence collection tool and method for collecting and appraising micro evidence |
| JP2017036987A (en) * | 2015-08-10 | 2017-02-16 | 株式会社エクシールコーポレーション | Fine object sampling tool and method for sampling and examining fine object |
| JP2019074336A (en) * | 2017-10-12 | 2019-05-16 | 株式会社エクシール | Microscopic material collecting tool |
| JP7402457B1 (en) | 2023-01-18 | 2023-12-21 | 日本ソフトウェアマネジメント株式会社 | Will sheets, appraisal sheets, will kits, and appraisal methods related to wills |
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