JP2012520080A5 - - Google Patents

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Publication number

JP2012520080A5

JP2012520080A5 JP2011554206A JP2011554206A JP2012520080A5 JP 2012520080 A5 JP2012520080 A5 JP 2012520080A5 JP 2011554206 A JP2011554206 A JP 2011554206A JP 2011554206 A JP2011554206 A JP 2011554206A JP 2012520080 A5 JP2012520080 A5 JP 2012520080A5

Authority

JP

Japan

Prior art keywords

reaction mixture

amplification reaction

double

additive

primer

Prior art date

2010-03-11

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

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• 239000011541 reaction mixture Substances 0.000 claims description 43
• 230000003321 amplification Effects 0.000 claims description 40
• 238000003199 nucleic acid amplification method Methods 0.000 claims description 40
• 239000000654 additive Substances 0.000 claims description 32
• 230000000996 additive effect Effects 0.000 claims description 28
• 108091034117 Oligonucleotide Proteins 0.000 claims description 23
• 238000006243 chemical reaction Methods 0.000 claims description 13
• 238000000034 method Methods 0.000 claims description 13
• 239000000203 mixture Substances 0.000 claims description 9
• 239000000523 sample Substances 0.000 claims description 9
• 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 8
• 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 8
• 238000000137 annealing Methods 0.000 claims description 8
• 230000000295 complement effect Effects 0.000 claims description 8
• 239000002773 nucleotide Substances 0.000 claims description 8
• 125000003729 nucleotide group Chemical group 0.000 claims description 8
• 230000001419 dependent effect Effects 0.000 claims description 6
• 230000002401 inhibitory effect Effects 0.000 claims description 5
• 230000004544 DNA amplification Effects 0.000 claims description 4
• 238000001514 detection method Methods 0.000 claims description 4
• 230000000694 effects Effects 0.000 claims description 4
• WCKQPPQRFNHPRJ-UHFFFAOYSA-N 4-[[4-(dimethylamino)phenyl]diazenyl]benzoic acid Chemical compound C1=CC(N(C)C)=CC=C1N=NC1=CC=C(C(O)=O)C=C1 WCKQPPQRFNHPRJ-UHFFFAOYSA-N 0.000 claims description 3
• 102100034343 Integrase Human genes 0.000 claims description 3
• 108010010677 Phosphodiesterase I Proteins 0.000 claims description 3
• 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims description 3
• 229920002521 macromolecule Polymers 0.000 claims description 3
• 125000003367 polycyclic group Chemical group 0.000 claims description 3
• 230000000977 initiatory effect Effects 0.000 claims 1
• JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
• 238000003556 assay Methods 0.000 description 2
• 239000003153 chemical reaction reagent Substances 0.000 description 2
• 238000002844 melting Methods 0.000 description 2
• 230000008018 melting Effects 0.000 description 2
• 238000012986 modification Methods 0.000 description 2
• 230000004048 modification Effects 0.000 description 2
• 239000003607 modifier Substances 0.000 description 2
• 238000003752 polymerase chain reaction Methods 0.000 description 2
• 238000012408 PCR amplification Methods 0.000 description 1
• 238000009396 hybridization Methods 0.000 description 1
• 230000037452 priming Effects 0.000 description 1
• 238000010839 reverse transcription Methods 0.000 description 1
• 230000001629 suppression Effects 0.000 description 1