JP2012254940A - Xyloglucan-containing composition, and manufacturing method of xyloglucan-containing sheet for pasting and xyloglucan-containing gel molded article - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a xyloglucan-containing composition from which a required gel molded article can be easily made, and provide a method for manufacturing a xyloglucan-containing sheet for pasting and a xyloglucan-containing gel molded article.SOLUTION: The xyloglucan-containing composition is characterized by containing water, xyloglucan, sucrose, and polyhydric alcohol, wherein pH is 3 or more.

Description

  The present invention relates to a xyloglucan-containing composition applicable to a substrate for adhesion to skin or mucosa.

  Conventionally, various external preparations are used for skin diseases and mucosal diseases. Among skin diseases, pressure ulcers (so-called bed sores) and burns are known as representative examples of intractable wounds.

  Decubitus is an ischemic skin necrosis caused by the body part in contact with the floor being continuously pressed against the floor by its own weight, and it can be used for patients with chronic diseases and malnutrition, long-term bedridden patients such as the elderly, etc. It is easy to generate. As the aging society progresses, the number of long-term bedridden people has increased, and the need for treatment at home has increased, so the need for pressure ulcer treatment has increased.

  In treating pressure ulcers and burns, active ingredients that prevent bacterial infection and active ingredients that promote granulation are administered to the affected area. In order to enhance the therapeutic effect, the affected area is prevented from coming into contact with the outside environment such as air, the contact between the affected area and the bacteria in the external environment is prevented, the bacterial infection prevention action is improved, or the affected area is moderately It is moistened to improve the granulation action. In addition, in order to enhance the above therapeutic effect, it is also effective to clean the affected area such as removing excess exudate from the affected area.

  In addition, mucosal diseases are caused by the secretion fluid covering the surface of the mucous membrane being dried and causing mucosal malfunction and functional deterioration, or by a wound. In the treatment of such mucosal diseases, it is effective to prevent contact between the mucous membrane and air while supplying the active ingredient to the affected part of the mucosa, as described above.

  For the treatment of such skin diseases and mucosal diseases, an ointment product or cream product kneaded with an active ingredient is applied to the affected area, or a sheet-like product such as a film containing the active ingredient is applied. While the active ingredient is supplied to the affected area, the contact between the affected area and the external environment is prevented, and the affected area is prevented from being dried to thereby wet the affected area. In addition, when the ointment is re-applied or the sheet-like base material is replaced, the affected part is cleaned when the ointment product is wiped from the affected part or when the film product is peeled off.

  However, when an ointment product or the like is applied, the affected area cannot be sufficiently covered, so that the affected area comes into contact with air, and there is a possibility that the effect of preventing bacterial infection and the effect of promoting granulation formation may not be sufficiently obtained. In addition, an ointment product or the like is spread on gauze or the like so as to sufficiently cover the affected area, or the ointment product or the like is further mixed with another ointment base and then spread to gauze or the like. However, such work requires skill and skill when it is extended so as not to soak into the gauze or when the amount is increased.

  Moreover, when using a sheet-like product, if the strength and elasticity of the sheet base material are insufficient, the product may be damaged at the time of application, or it may be difficult to sufficiently bring the active ingredient into contact with the affected area. At this time, if the sheet base material is firmly adhered to the skin or the like, it causes pain to the patient when the base material is peeled off.

  As described above, if work is troublesome or pain is caused to the patient, it leads to a decrease in compliance, and it is difficult to sufficiently exert the therapeutic effect. In particular, when pressure ulcers or burns are severe, etc., treatment is prolonged, and replacement of ointment products, sheet-like products, etc. is repeated many times.

  Therefore, it has been proposed to use a composition containing xyloglucan which is a polysaccharide. For example, it is known that a composition containing a polysaccharide containing xyloglucan, sucrose, an acid having a carboxyl group, and a polyhydric alcohol is used as a skin patch sheet (Patent Document 1). According to such a composition, since the composition has elasticity while retaining water, it can sufficiently cover an affected area such as a skin disease, and prevents contact between the affected area and air and also prevents dryness of the affected area. In addition, the affected area can be moistened. Moreover, since it does not soak into gauze or the like and it is not necessary to increase the dose, it is possible to save the labor of the nurse and further reduce the patient's pain when peeling off.

  However, such a composition cannot sufficiently adjust the gelation speed, and it may be difficult to prepare a desired gel molded body using the composition.

JP 2008-50298 A

  In view of the above-described problems, an object of the present invention is to provide a xyloglucan-containing composition, a xyloglucan-containing patch sheet, and a method for producing a xyloglucan-containing gel molded body, which can easily prepare a desired gel molded body.

  As a result of intensive studies to overcome the above-mentioned problems, the inventors of the present invention can easily prepare a desired gel molded article by containing xyloglucan, sucrose, and a polyhydric alcohol and adjusting the pH to a predetermined value. The inventors have found that a xyloglucan-containing composition can be obtained, and have arrived at the present invention.

That is, the xyloglucan-containing composition according to the present invention contains water, xyloglucan, sucrose, and a polyhydric alcohol, and has a pH of 3 or more.
Here, the polyhydric alcohol refers to a compound having two or more hydroxyl groups (divalent or higher alcohol). Polyhydric alcohols include those in which a hydroxyl group is bonded to a carbon atom that does not form a benzene ring, as well as those in which a hydroxyl group is bonded to carbon that forms a benzene ring.

  According to such a configuration, since water, xyloglucan, sucrose, and polyhydric alcohol are contained and the pH is 3 or more, the gelation rate of the xyloglucan-containing composition can be easily adjusted. The gel molded body can be easily prepared. Further, when the xyloglucan-containing composition has a pH of 3 or more, sufficient gelation can be achieved. Therefore, the xyloglucan-containing composition can easily prepare a desired gel molded body. In addition, by containing water, sucrose, and polyhydric alcohol in this manner, the xyloglucan-containing composition can be gelled without requiring an acid containing a carboxyl group.

  In the xyloglucan-containing composition according to the present invention, the polyhydric alcohol preferably contains at least one of polyethylene glycol or polyphenols.

  Thus, the polyhydric alcohol contains polyethylene glycol or polyphenols, whereby gelation of the composition can be further promoted. Moreover, when polyhydric alcohol contains polyethyleneglycol and polyphenols, such gelation can be further promoted. Furthermore, since the polyhydric alcohol is polyethylene glycol, it is possible to form a gel with a good touch, and when the polyhydric alcohol is a polyphenol, a hard gel can be formed. It becomes easy to prepare a gel molded article having desired physical properties.

  Further, in the xyloglucan-containing composition according to the present invention, the xyloglucan-containing composition is 0.1 to 5 wt%, the sucrose is 5 to 55 wt%, and the polyhydric alcohol is 0 It is preferable to contain 0.05 to 50 weight%.

  Moreover, in the xyloglucan-containing composition according to the present invention, the polyhydric alcohol contains a polyphenol, and 0.05 to 4% by weight of the polyphenol with respect to the entire xyloglucan-containing composition. Is preferred.

  The xyloglucan-containing composition according to the present invention is preferably for adhering to the skin or mucous membrane.

  Thereby, contact with the affected part of skin disease or mucous membrane disease and air can be prevented, and the affected part can be moistened while preventing drying, and the therapeutic effect can be enhanced. In addition, disease can be prevented by preventing dryness and the like. Furthermore, the pain of the patient at the time of removal can also be reduced.

  Moreover, it is preferable that the xyloglucan containing composition which concerns on this invention is a sheet form.

  Thereby, it becomes possible to cover the affected area of skin diseases and mucosal diseases over a wider range.

  The xyloglucan-containing patch sheet according to the present invention is characterized in that the xyloglucan-containing composition and a sheet-like base material are laminated.

  Thereby, since the intensity | strength and elasticity of the said xyloglucan containing composition can be reinforced, at the time of sticking to skin and a mucous membrane, durability improves more.

  The method for producing a xyloglucan-containing gel molded product according to the present invention is obtained by mixing water, xyloglucan, sucrose, and polyhydric alcohol so as to have a pH of 3 or more to obtain a sol-like mixed solution. The mixed solution is formed by gelation.

  Thereby, a xyloglucan containing gel molded object can be manufactured easily.

  In the method for producing a xyloglucan-containing gel molded article according to the present invention, the mixed solution is prepared by mixing the water, the mixed solution of xyloglucan and the sucrose, and the polyhydric alcohol. Is preferred.

  The mixed solution of water, xyloglucan and sucrose has a relatively slow gelation rate, and the gelation rate can be increased by mixing a polyhydric alcohol with this. That is, since the gelation speed of the mixed solution can be adjusted by the addition concentration and addition timing of the polyhydric alcohol, the preparation of the xyloglucan-containing gel molded product becomes easier.

Moreover, in the manufacturing method of the xyloglucan containing gel molding which concerns on this invention, it is preferable to make it gelatinize, making the said mixed solution adhere to a to-be-adhered area | region.
Here, “gelling while adhering” means that after the mixed solution is adhered to the adherend region, the change of the mixed solution from the sol state to the gel state is completed in the adhering region. In other words, “gelling while adhering” includes a mode in which the sol-like mixed solution is in a sol state when adhering to the adherent region, and already has a certain degree when adhering to the adherent region. A mode in which gelation is progressing is also included.

  Thereby, it becomes possible to cover the affected part appropriately according to the shape and size of the affected part of the skin or mucosal disease.

  As described above, according to the present invention, it is possible to obtain a xyloglucan-containing composition that facilitates the preparation of a desired gel molded body.

Graph showing change in storage elastic modulus (G ') with respect to time A photograph showing the effect of promoting the reduction of the wound area with a xyloglucan-containing patch sheet

  The xyloglucan-containing composition according to this embodiment contains water, xyloglucan, sucrose, and a polyhydric alcohol, and has a pH of 3 or more.

  The xyloglucan is a nonionic polysaccharide having glucose and xylose as constituent sugars, and has a basic structure in which xylose is bonded as a side chain to a main chain in which glucose is β-1,4 bonded. In this specification, the term “xyloglucan” means both high-molecular (natural) xyloglucan and low-molecular-weight xyloglucan.

  The high molecular xyloglucan is a xyloglucan derived from a natural product and has a molecular weight of about 50,000 to 1,000,000. Such high molecular weight xyloglucan can be obtained, for example, by extracting from cell walls of higher plants such as peas, soybeans, poplars, rice, bamboo shoots, tamarind seeds, and the like. In addition, high molecular weight xyloglucan can be extracted from plant tissues using water, or when it is difficult to extract with water, it can be extracted using an alkaline aqueous solution, but it can be extracted more easily. Regardless of whether or not extraction is possible with water, it is preferable to perform extraction using an aqueous alkaline solution.

  The polymer xyloglucan obtained by such extraction can be further purified by alcohol precipitation, ion exchange chromatography, various types of chromatography utilizing differences in hydrophobicity or affinity, etc., if necessary. Good.

  Among the polymer xyloglucans, a polymer xyloglucan derived from tamarind seeds (tamarind seed xyloglucan) is obtained as a commercially available product (for example, DSP Gokyo Food & Chemical Co., Ltd .: Griloid 6C). Such a commercial product can be dissolved in water, ethanol and the like can be mixed with this aqueous solution to separate and precipitate xyloglucan, and further purified for use.

  Furthermore, high molecular weight xyloglucan may be partially decomposed to reduce the molecular weight for easy use.

  A low molecular weight xyloglucan is a xyloglucan obtained by reducing the molecular weight of a high molecular weight xyloglucan. Such low-molecular-weight xyloglucans can be isolated by physical methods (for example, reduction of molecular weight by homogenizer, etc.), chemical methods (for example, reduction of molecular weight by acid hydrolysis, etc.), enzymatic degradation, or the like. It can be obtained by a known method such as chemical synthesis.

  For example, when low molecular weight xyloglucan is obtained from tamarind seed xyloglucan by enzymatic decomposition, a plant tissue-disintegrating enzyme having β-1,4-glucanase activity is used, and the addition amount, pH, temperature according to the characteristics of the enzyme The enzyme decomposition reaction can be carried out by appropriately setting the time.

  When cellulase is used as such a plant tissue disrupting enzyme, for example, tamarind seed xyloglucan in water and 0.01 to 2.0 parts by weight of cellulase with respect to 100 parts by weight of the tamarind seed xyloglucan. The resulting mixture is subjected to an enzymatic degradation reaction at pH 3 to 7 and a temperature of 35 to 60 ° C. for 3 to 96 hours. And after completion | finish of reaction, a low molecular weight xyloglucan whose molecular weight is 5000 or less can be obtained by deactivating cellulase and refine | purifying a reaction product by methods, such as a chromatography. Moreover, a side chain can also be partially decomposed and the method (partial decomposition method) includes an enzymatic or chemical method.

  The xyloglucan may be either a high-molecular xyloglucan or a low-molecular xyloglucan, or a mixture of both, and a mixture of xyloglucans derived from a plurality of types of plants. Also good.

  The sucrose is a disaccharide constituted by two D-glucose molecules linked by α-1,4. Such sucrose is generally called sugar and has been used as a seasoning for a long time. The form of such sucrose is not particularly limited, and for example, it can be used in the form of granule, sucrose, purified sucrose and the like. These may be unground or finely pulverized.

  The polyhydric alcohol is a compound having two or more hydroxyl groups (alcohol having two or more hydroxyl groups). The polyhydric alcohol is formed by bonding a hydroxyl group to a carbon atom that does not constitute a benzene ring, as well as benzene. Also included are those in which a hydroxyl group is bonded to the carbon constituting the ring.

  Examples of the former polyhydric alcohol having a hydroxyl group bonded to a carbon atom that does not constitute a benzene ring include glycerol, polyethylene glycol, butylene glycol, pentylene glycol, and the like, and one or two selected from these The above can be mixed and used.

Among these polyhydric alcohols, for example, glycerol is a trivalent polyhydric alcohol represented by CH ₂ OH—CHOH—CH ₂ OH, and polyethylene glycol is H — [— O—CH ₂ —CH ₂ —. ] A polymer represented by _n- OH, all having high hydrophilicity. These polyhydric alcohols may be either chemically synthesized products or plant-derived polyhydric alcohols, but are preferably chemically synthesized polyhydric alcohols.

  By containing such a polyhydric alcohol, the gelation rate of the xyloglucan-containing composition can be easily adjusted. Of these polyhydric alcohols, polyethylene glycol is preferred from the viewpoint of easy adjustment of the gelation rate.

  Moreover, polyphenols are mentioned as the latter polyhydric alcohol which the hydroxyl group couple | bonded with the carbon which comprises a benzene ring. Such polyphenols can be used alone as polyhydric alcohols, or polyphenols can be used together with the polyhydric alcohols described above.

  The polyphenol means a series of compounds having a phenol structure having a plurality of hydroxyl groups. The polyphenol may be either a chemically synthesized product or a polyphenol derived from a plant raw material, and is preferably a polyphenol derived from a plant raw material.

  Such polyphenols derived from plant materials include, for example, tea leaves such as green tea (matcha sencha, etc.), black tea, oolong tea, puer tea (black tea), mate tea, white vegetables, pericarps and seeds, grape pericarps and seeds (Red wine, red grape juice, etc.), apples, strawberries, peaches, pears, onion peels, chestnuts, burdock, coffee beans, cacao beans and other plants. Specifically, it is contained in an extract (or extract) extracted from the plant with warm water or the like, and by appropriately purifying the extract, desired polyphenols (for example, tannins, catechins, Proanthocyanidins (or protocyanidine), flavonoids, etc.) can be obtained.

  Examples of plant-derived polyphenols include flavones (rutin, luteolin, etc.), furanolol (quercetin, kaempferol, etc.), flavanones (hesperidin, naringin, etc.), flavanols (catechins), anthocyanidins (cyanidine, etc.), Isoflavones (genistein, genistin, daidzein, etc.), tannins (catechol tannin, pyrogallol tannin, etc.), tannic acid, chlorogenic acid, isochlorogenic acid, etc., catechins ((+) catechin, (+) gallocatechin, (+) Epicatechin and its gallic ester, (+) epigallocatechin and its gallic ester, thealvidin and its gallic ester, prodelphinidin and its gallic ester, and the like. As polyphenols derived from plant materials, catechins are preferred.

  As the polyphenols used in the present invention, the above-mentioned extract (or extract) itself may be used, or a liquid obtained by concentrating polyphenols from the extract may be used. Polyphenols may be purified from the extract (or extract) and used. Moreover, polyphenols can also be used as a 1 type, or multiple types of mixture. Ordinary green tea, black tea, oolong tea, puer tea (black tea), mate tea, red wine, red grape juice, coffee, etc., or commercially available drinking water thereof may be used as they are. In addition, from the viewpoint that polyphenols can be used efficiently, it is preferable to use an extract rather than using commercially available drinking water as it is. The polyphenols used in the present invention may be either powder or aqueous solution.

  By containing such a polyhydric alcohol, the gelation rate of the xyloglucan-containing composition can be easily adjusted as described above. In addition, when polyphenols are contained as the polyhydric alcohol, gelation of the xyloglucan-containing composition can be promoted at a lower concentration than when polyethylene glycol is contained.

  In addition, the polyhydric alcohol formed by bonding a hydroxyl group to carbon that does not form a benzene ring and the polyhydric alcohol formed by bonding a hydroxyl group to carbon that forms a benzene ring are used in combination. Thus, not only can the gelation rate be more easily adjusted, but also physical properties such as tactile sensation can be improved. Specifically, it is preferable to use a combination of polyethylene glycol and polyphenols.

  About the compounding quantity of xyloglucan, sucrose, and a polyhydric alcohol in the said xyloglucan containing composition, the said xyloglucan containing composition contains 0.1 to 5weight% of xyloglucan with respect to this whole composition. The content is preferably 1.5 to 3% by weight. When the xyloglucan-containing composition contains 0.1% by weight or more of xyloglucan, the gel strength can be increased. On the other hand, when the xyloglucan-containing composition contains 5% by weight or less of xyloglucan, the xyloglucan-containing composition can be contained in a viscosity range that is easy to handle.

  Moreover, it is preferable that the said xyloglucan containing composition contains 5-55 weight% of sucrose with respect to this whole composition, and it is more preferable to contain 5-40 weight%. Gelation can be accelerated | stimulated because the said xyloglucan containing composition contains sucrose 5weight% or more. On the other hand, when the xyloglucan-containing composition contains 55% by weight or less of sucrose, the gelation speed and gel physical properties are controlled, the gel hardness is adjusted, and the feel of the gel is too low. Can be suppressed.

  Moreover, it is preferable that the said xyloglucan containing composition contains 0.05-50 weight% of polyhydric alcohol with respect to the whole xyloglucan containing composition, and it is more preferable to set it as 0.5-30 weight%. Gelation can be accelerated | stimulated because the said xyloglucan containing composition contains a polyhydric alcohol 0.05weight% or more. On the other hand, when the xyloglucan-containing composition contains 50% by weight or less of polyhydric alcohol, the gelation speed and gel properties are controlled, the gel hardness is adjusted, and the feel of the gel is excessively lowered. This can be suppressed.

  When polyethylene glycol is contained as the polyhydric alcohol, the xyloglucan-containing composition preferably contains 2 to 20% by weight of polyethylene glycol with respect to the entire composition. Gelation can be accelerated | stimulated because the said xyloglucan containing composition contains polyethyleneglycol 2weight% or more. On the other hand, when the xyloglucan-containing composition contains 20% by weight or less of polyethylene glycol, the gelation speed and gel physical properties are controlled, and the gel becomes too hard or the feel of the gel is too low. Can be suppressed.

  Moreover, when polyphenols are contained as the polyhydric alcohol, it is preferable that the xyloglucan-containing composition contains 0.05 to 4% by weight of polyphenols with respect to the entire composition. Thus, gelling can be accelerated | stimulated by containing 0.05 weight% or more. On the other hand, when the gel composition contains 4% by weight or less of polyphenols, the gelation speed and gel physical properties are controlled, the gel hardness is adjusted, and the gel feel is excessively lowered. Can be suppressed.

  The pH of the xyloglucan-containing composition is 3 or more. When the pH is 3 or more, the gelation rate of the xyloglucan-containing composition can be easily adjusted, and a desired gel molded body can be easily prepared. Moreover, useless coloring can also be prevented. In addition, by containing water, sucrose, and a polyhydric alcohol, the pH is set to 3 or more, whereby the xyloglucan-containing composition can be gelated without requiring an acid containing a carboxyl group. On the other hand, the upper limit of the xyloglucan-containing composition is 14 or less, which is the upper limit of pH. The pH of the xyloglucan-containing composition is preferably 10 or less. When the pH is 10 or less, useless coloring can be prevented.

  Such pH can be adjusted by adding an acid, an alkali, a pH adjuster or the like to the xyloglucan-containing composition. In addition, as described above, the acid can be gelled without requiring an acid containing a carboxyl group as described above, and therefore an acid containing no carboxyl group can be contained.

  The gel properties of the xyloglucan-containing composition are preferably those having adhesion to the skin and appropriate strength. The gel composition becomes transparent if each component is sufficiently dissolved. However, it does not necessarily need to be transparent, and includes a case where some of the components in the gel composition remain undissolved. Further, as will be described later, a coloring agent or the like can be added and colored as necessary.

  In addition, the said xyloglucan containing composition can be made to contain components other than the above as needed.

  In addition, for example, the xyloglucan-containing composition can contain a plasticizer, whereby a smooth gel having a good touch is obtained. Examples of such plasticizers include liquid paraffin, ethylene glycol and oligomers thereof, propylene glycol and oligomers thereof, glycerin derivatives obtained by adding ethylene oxide and propylene oxide to polyglycerin and glycerin, sorbitol, pentaerythritol, and the like. Etc.

  In addition, for example, the xyloglucan-containing composition can contain various pharmaceutically acceptable components, and are usually used in, for example, active ingredients effective for the treatment of skin diseases and mucosal diseases, and external preparations. Such a component can be contained.

  For example, a wound treatment component or a component having an action such as disinfection, sterilization, antibacterial, or antibacterial can be contained. Specifically, as the component, sulfadiazine silver, silver colloid, silver ion, popidone iodine, hypoxia Examples thereof include alcohols such as sodium chlorate and ethanol.

  For example, when the above-described silver colloid is used, a colloid in which silver particles are finely divided to about 10 nm and dispersed in an aqueous solution can be used. This silver colloid may contain polyvinylpyrrolidone as a stabilizer, and an antibacterial effect can be expected as a water-soluble colloid. For example, a silver colloid solution (trade name: NANOVER PR-WB14R, manufactured by Nippon Ion Co., Ltd., silver fine particle content: 1% by weight) can be used as a commercial product, but is not limited thereto.

  In addition, the xyloglucan-containing composition may contain components having anti-inflammatory action, moisturizing action, vasoconstrictive action, etc., specifically, glycyrrhizic acid, procaine chloride, lidocaine, cortisone, betamethasone. , Aloe, ephedrine hydrochloride, sodium hyaluronate, olive oil and the like.

  In addition, components that are usually used in external preparations can be contained, and specifically, fragrances, pigments, buffers, polysaccharides other than xyloglucan, and the like can be contained. Specific examples of such polysaccharides include xanthan gum, gellan gum, alginates, pectin, agar, crystalline cellulose, gelatin, starch, dextrin and the like.

  The method for producing a xyloglucan-containing gel molded product according to the present invention comprises mixing water, xyloglucan, sucrose, and polyhydric alcohol so as to have a pH of 3 or more, and a sol-like mixed solution (mixture of four components). Solution) and the resulting mixed solution is gelled to form.

  Thereby, the outstanding xyloglucan containing gel molded object can be manufactured easily.

  Specifically, a mixture of xyloglucan, sucrose and polyhydric alcohol in powder form, or a mixture prepared by adding deionized water to a slurry and mixing them in deionized water while stirring well After preparing a mixed solution by dispersing and dissolving, heat to about 70 to 90 ° C, and after heating, pour into an appropriate mold, or apply to the diseased part of the skin or mucous membrane, and then cool by cooling. Can be molded.

  Moreover, immediately after preparing the mixed solution of the above four components, it is sol-like and has fluidity, but it gels with time. Specifically, it gels within approximately one hour. Accordingly, by mixing the above four components and applying the obtained sol-like mixture to the skin and mucous membrane before the mixture gels, the xyloglucan-containing composition becomes a gel after the above time has elapsed. Changes and adheres to the skin and mucous membranes. Thereby, a diseased part can be covered.

  Here, the gelation rate is not so high even when 40% by weight or less of sucrose is added to the aqueous solution of xyloglucan, but when the polyhydric alcohol is added to the aqueous solution of xyloglucan, the gelation rate increases rapidly. Therefore, it is preferable to prepare the mixed solution of the above four components by mixing a mixed solution of water, xyloglucan and sucrose with a polyhydric alcohol. Thereby, since the gelatinization speed | rate of a xyloglucan containing composition can be adjusted with the addition timing of a polyhydric alcohol, preparation of a xyloglucan containing gel molded object becomes easier. Therefore, since the gelation speed can be adjusted according to the convenience of the nurse, the work load can be reduced.

  In this case, specifically, for example, after adding xyloglucan to deionized water and dissolving by heating as described above, sucrose and an appropriate plasticizer are further added to the aqueous solution and dissolved, A mixed solution of xyloglucan and sucrose can be prepared, and polyhydric alcohol can be added to this solution and kneaded (mixed).

  The above-mentioned mixed solution of the four components can also be prepared by mixing a mixed solution of water and xyloglucan with a mixed solution of water, sucrose and a polyhydric alcohol. Specifically, for example, an aqueous solution prepared by dispersing and dissolving xyloglucan powder in deionized water while stirring well, sucrose and polyhydric alcohol are added to deionized water, mixed, and stirred well. The prepared aqueous solution can be prepared, and both aqueous solutions can be mixed at a predetermined ratio so as to be the amount of each of the components exemplified above. Then, the obtained four-component mixed solution is poured into, for example, a 4 × 4 cm square mold placed on a hot plate and heated at 37 ° C., whereby the xyloglucan-containing gel molded body is formed into a sheet form. Can be molded.

  The reason why gelation is possible by heating at 37 ° C. is as follows. That is, the higher the concentration of the three components of xyloglucan, sucrose, and polyhydric alcohol, the higher the temperature of the mixed solution of the above four components is. However, for example, in the case of a mixed solution of four components in which the concentration of the above three components is relatively low, by heating at 37 ° C., moisture is lost over time with heating. Since the concentration of the three components in the solution increases, the mixed solution can be gelled. In addition, from this, by applying the mixing liquid, it is possible to gel the mixed solution without additional heating by heating due to the skin temperature and losing moisture over time.

  Thus, when the xyloglucan-containing gel molded product is in a sheet form, that is, the xyloglucan-containing composition is in a sheet form, it is possible to cover the affected area of skin diseases and mucosal diseases over a wider range.

  Moreover, the sheet-like xyloglucan-containing gel molded body (xyloglucan-containing composition) and a sheet-like base material such as a film are laminated to form a xyloglucan-containing sticking sheet. According to such a xyloglucan-containing patch sheet in which the gel-like xyloglucan composition and a sheet-like base material are laminated, the strength of the xyloglucan-containing composition can be reinforced. Durability is further improved when it is applied to skin and mucous membranes.

  Moreover, in the manufacturing method of an above-mentioned xyloglucan containing gel molding, it is also preferable to make it gelatinize, making the said mixed solution adhere to a to-be-adhered area | region. Thereby, according to the shape of the affected part in skin or mucous membrane, it becomes possible to cover an affected part appropriately.

  For example, the sol-like mixed solution is gelled while being applied to the skin or mucous membrane as described above, and the mixed solution is sprayed onto the affected area while the mixed solution is in the sol form, or the mixed solution is gargleed in the mouth. By doing so, the mixed solution can be gelled in the sprayed portion or in the oral cavity to form a xyloglucan-containing gel molded body. Thus, it is also preferable to use a xyloglucan-containing gel composition as a spray or a gargle. Thereby, the xyloglucan-containing composition can be adhered and covered even on an affected part that is difficult to apply.

  As described above, the xyloglucan-containing composition changes from a sol to a gel and the time required for the change is as described above. Can be preferably used. That is, instead of gelling the xyloglucan-containing composition in advance into a sheet form, the xyloglucan composition contains an oleaginous base such as petrolatum or an emulsion base to make the cream or skin or It can be made into a sheet by applying to the mucous membrane and evaporating moisture at the body temperature of the skin or mucous membrane.

  In addition, since the xyloglucan-containing composition is in a gel form, it can exhibit an elastic force, and thus is excellent in protecting the affected area. Furthermore, the physical properties of the gel can be easily controlled by the composition and the preparation method, various physical properties can be imparted, and it has appropriate strength, flexibility, and progressability, so that the feeling of use on the skin and mucous membrane is good. Moreover, since the xyloglucan-containing composition can prevent contact between the affected area and the external environment, it is excellent in terms of hygiene.

  In addition, when used for wound treatment, even if the xyloglucan-containing composition comes into contact with the exudate in the affected area, the gel physical properties hardly change, and thus the gelled xyloglucan-containing composition is difficult to disintegrate. In addition, since the xyloglucan-containing composition is transparent, it is effective because the state of the disease can be confirmed even after application on the skin or mucous membrane. Moreover, since the said xyloglucan containing composition contains the thing derived from natural products, such as xyloglucan and sucrose, it is excellent in safety | security and is also friendly to an environment.

EXAMPLES The present invention will be specifically described below with reference to examples, but the present invention is not limited to these examples. In the following examples, xyloglucan derived from tamarind seeds (trade name “Glyroid 6C”, manufactured by DSP Gokyo Food & Chemical Co., Ltd.) was used as xyloglucan. Further, the xyloglucan was used after further purification by dissolving in an aqueous solution and then fractionally precipitating with ethanol. As sucrose, sucrose (trade name: purified sucrose, manufactured by Wako Pure Chemical Industries, Ltd.) was used.
Test Example 1 Effect of promoting gelation by polyethylene glycol and polyphenols

  The effect of promoting gelation was evaluated by measuring the time dependency of the storage modulus (G ′) of the prepared sample. A dynamic stress rheometer model: SR-2000 (manufactured by Rheometric Scientific) was used as a measuring device, and dynamic viscoelasticity was measured at an angular frequency of 1.0 rad / s in a predetermined temperature range. At that time, the stress was controlled so as to maintain the minute stress (0.1 Pa) condition in order to ensure the measurement within the linear range. A parallel plate was used for the measurement.

  Here, G ′ refers to the real part of the complex rigidity, and is related to the work that the gel-like substance exerts on the outside when the external force is removed after the gel-like substance is deformed. Yes, it is an index of the degree of energy storage. Therefore, in the present invention, an increase in G ′, which is an elastic term, was used as an index for gelation.

  According to the prescription in Table 1, xyloglucan was dissolved in deionized water at 80 ° C., and polyphenol as a liquid A, sucrose and polyhydric alcohol (trade name “Sanphenon”, manufactured by Taiyo Chemical Co., Ltd., polyphenol content 87.9% by weight) Was used, it was dissolved in deionized water at 80 ° C. to obtain Liquid B. Next, liquid A and liquid B were mixed in a test tube while heating to 80 ° C., and dynamic viscoelasticity at 37 ° C. was measured. When polyethylene glycol 400 (PEG 400: trade name “polyethylene glycol # 400”, manufactured by Nacalai Tesque) was used, it was added as C liquid after mixing A liquid and B liquid. The change of G 'with respect to time after mixing is shown in FIG.

  As shown in FIG. 1, in Comparative Example 1 in which neither polyethylene glycol nor polyphenols were added, G ′ did not change and gelation did not occur. However, G ′ rapidly increased due to addition of polyphenols or addition of polyethylene glycol. The gelation was promoted.

Test Example 2 Gelling promotion effect of polyphenols (1)
According to the formulation of Table 2, each raw material was dissolved in deionized water at 80 ° C. to obtain A liquid and B liquid. While heating to 80 ° C., liquid A and liquid B were mixed in a test tube. The gelation promoting effect of polyphenols at 37 ° C. or 10 ° C. was evaluated. In the evaluation, the case where the gelation rate was fast (when rising was seen in the above-mentioned time-G ′ graph) was marked with ◯, and the case where the gelation rate did not change was marked with ×. The results are shown in Table 2.

  As is apparent from Table 2, gelling was promoted in Examples 3 to 7 in which 0.05% by weight or more of polyphenols were added as compared with Comparative Example 2 in which no polyphenols were added. In Examples 4 to 7 in which 0.1% by weight or more was added, gelation was further promoted.

Test Example 3 Gelling promotion effect of polyphenols (2)
Similarly to Test Example 2, the sucrose concentration and xyloglucan concentration were changed in accordance with the formulation in Table 3, and A solution and B solution were mixed, and the gelling promotion effect of polyphenols was the same as in Test Example 2. evaluated. The results are shown in Table 3.

  As is clear from Table 3, in Examples 8 to 10 in which polyphenols were added as compared with Comparative Examples 3, 4 and 5 in which no polyphenols were added, regardless of the xyloglucan concentration and sucrose concentration. , Gelation was promoted.

Test Example 4 Effect of promoting the gelation of polyethylene glycol (1)
In the same manner as in Test Example 2, the liquid A and the liquid B were mixed according to the formulation in Table 4, and the gelation promoting effect of polyethylene glycol was evaluated in the same manner as in Test Example 2. The results are shown in Table 4.

  As apparent from Table 4, gelation was promoted in Examples 11 to 14 in which polyethylene glycol was added in an amount of 5% by weight or more as compared with Comparative Examples 6 and 7 in which polyethylene glycol was not added. In addition, the higher the sucrose concentration, the more gelation-promoting effect was obtained even at a lower concentration of polyethylene glycol.

Test Example 5 Gelation promotion effect of polyethylene glycol (2)
According to the formulation of Table 5, A liquid and B liquid were mixed, and the gelation promoting effect of polyethylene glycol was evaluated in the same manner as in Test Example 2 when the xyloglucan concentration was relatively high. The results are shown in Table 5.

  As is clear from Table 5, in Examples 15 to 18 in which polyethylene glycol was added in an amount of 2% by weight or more as compared with Comparative Examples 8 and 9 in which polyethylene glycol was not added, gelation was promoted. In Examples 15 to 18 in which not less than% by weight was added, gelation was further promoted. In addition, as in Table 4 above, the higher the sucrose concentration, the better the gelation promoting effect even when the polyethylene glycol concentration was low.

Test Example 6 Gelatin Acceleration Effect of Glycerol According to the formulation in Table 6, solution A and solution B were mixed, and the gelation promotion effect of glycerol was evaluated in the same manner as in Test Example 2. The results are shown in Table 6.

  As apparent from Table 6, gelation was promoted in Examples 19 and 20 to which glycerol was added, as compared with Comparative Examples 10 and 11 to which glycerol was not added. In addition, the higher the sucrose concentration, the better the gelation promoting effect even at a lower concentration of glycerol.

Test Example 7 Gelatin Promoting Effect of Butylene Glycol According to the formulation of Table 7, A liquid and B liquid were mixed, and the gelling promoting effect of butylene glycol was evaluated in the same manner as Test Example 2. The results are shown in Table 7.

  As is apparent from Table 7, gelation was promoted in Examples 21 to 24 to which butylene glycol was added, as compared with Comparative Examples 12 and 13 to which butylene glycol was not added. In addition, the higher the sucrose concentration, the better the gelation promoting effect even when the butylene glycol concentration was low.

Test Example 8 Influence on Gelation Promotion Effect by Addition of Silver Colloid According to the prescription in Table 8, A liquid and B liquid are mixed, and the influence of silver colloid addition on the gelation promotion effect by polyethylene glycol is shown in Test Example 2 and Evaluation was performed in the same manner. As the silver colloid, a silver colloid solution (trade name “NANOVER PR-WB14R”, manufactured by Nippon Ion Co., Ltd., silver fine particle content: 1% by weight) was used. The results are shown in Table 8.

  As is clear from Table 8, gelation was promoted by polyethylene glycol in Example 25 to which silver colloid was added as in Example 14 to which silver colloid was not added. The gelation rate was similar to that in Example 14, and the silver colloid did not adversely affect the gelation rate.

Test Example 9 Effect as a base material of an external preparation for wound treatment The effect as a wound base material was performed on a frostbite model rat using a sheet of xyloglucan-sucrose-polyethylene glycol. First, according to the prescription in Table 9, 6 g of a solution in which 2% by weight of xyloglucan, 33% by weight of sucrose, and 6.5% by weight of PEG400 were mixed was poured into a 3 cm × 4 cm mold, A composition was prepared. Next, a single sheet of polyurethane film (trade name “Cateliep”, manufactured by Nichiban) was overlaid on the prepared sheet-like composition to prepare an adhesive sheet of Example 26.

  On the other hand, a comparative example 14 was obtained by simply stacking 6 sheets of gauze. A sheet-like composition is prepared in the same manner as in Example 26 except that xyloglucan is 2% by weight, sucrose is 33% by weight and PEG400 is not used, and the polyurethane film is laminated thereon. Thus, a sticking sheet of Comparative Example 15 was prepared.

  Then, 7 week-old Wistar male rats were anesthetized by intraperitoneal injection of a pentobarbital aqueous solution, and then the right back hair was shaved and the skin was excised into a circle having a diameter of 1.5 cm. A wound was formed by covering the wound surface with a wrap and pressing a cylindrical brass tube filled with acetone-dry ice against the abdomen with its own weight. Four pieces of gauze were placed on the wound part overnight and application of each sheet was started from the next day.

Each said sheet | seat was changed every other day and the wound part was evaluated. The wound size is the wound area (a × bcm ² ) calculated by the major axis (acm) × the minor axis (bcm) of the wound area, and the wound in each elapsed day relative to the wound area immediately after the treatment (elapsed days 0). The area ratio was evaluated by calculating the ratio. The wound area was measured with a ruler. In addition, the condition of the wound surface was comprehensively evaluated by observation and condition. The results are shown in Table 10 and FIG.

  Compared with the comparative example 14, in Example 26, the reduction | decrease of the wound surface was quick and the state of the wound surface was also favorable. As a result, the sheet using the composition containing xyloglucan-sucrose-polyethylene glycol was shown to be effective for wound treatment. In Comparative Example 15, the base material did not gel and the mixture could not be placed on the wound surface, and thus could not be evaluated.

Test Example 10 Effect of Sheet Containing Silver Colloid Evaluation was performed using the same model as Test Example 9. In the same manner as in Example 26 described above except that xyloglucan was 2% by weight, sucrose was 33% by weight, PEG400 was 6.5% by weight, and the silver colloid solution was 0.5% by weight according to the formulation of Table 11. The sticking sheet of Example 27 was created.

Then, using Example 27 and Example 26 described above, the condition of the wound surface was comprehensively evaluated by observation and condition in the same manner as in Test Example 9. The results are shown in Tables 12 and 13.

  Compared to Example 26, in the sheet of Example 27 containing silver colloid, since the odor was relatively attenuated, it was considered that infection was suppressed by the action of silver colloid as an active ingredient. It is done. As a result, it was shown that the addition of silver colloid is effective for improving the therapeutic effect of the wound treatment sheet. In Example 27, in addition to the antibacterial action of the silver colloid, the sheet made of xyloglucan-sucrose-polyethylene glycol promoted the bacterial infection prevention action and granulation formation action, so that the therapeutic effect was enhanced. it is conceivable that.

Test Example 10 Effect of pH on Sheet According to the formulation of Example 26 shown in Tables 9 and 11, xyloglucan was 2 wt%, sucrose was 33 wt%, PEG400 was 6.5 wt%, and water was 18.5 wt% % To prepare a mixed solution with adjusted pH. The pH of the mixed solution was adjusted to 1.5, 3, 8, and 11, and the pH was adjusted by adding a hydrochloric acid solution or a sodium hydroxide solution. The mixed solution was put into a retort pouch and heated at 121 ° C. for 30 minutes. Thereafter, after standing at 10 ° C. for 24 hours, the degree of gelation was evaluated. The results are shown in Table 14. In addition, Table 14 shows the results of evaluating the pH of Example 26 in which hydrochloric acid and sodium hydroxide were not added and the pH was not adjusted, and the degree of gelation of Example 26 in the same manner as described above. Show.

  In Comparative Example 16, the result that gelation did not occur was obtained. Further, in Comparative Example 16, there was a tendency for a change in color tone to occur. Furthermore, since there was a tendency for color change to occur in Example 30, it was found that the pH was preferably 10 or less, and more preferably 8 or less.

Claims (10)

  A xyloglucan-containing composition comprising water, xyloglucan, sucrose, and a polyhydric alcohol, and having a pH of 3 or more.   The xyloglucan-containing composition according to claim 1, wherein the polyhydric alcohol contains at least one of polyethylene glycol or polyphenols.   3. The xyloglucan-containing composition as a whole contains 0.1 to 5% by weight of the xyloglucan, 5 to 55% by weight of the sucrose, and 0.05 to 50% by weight of the polyhydric alcohol. A xyloglucan-containing composition as described in 1. above.   The xyloglucan-containing composition according to claim 3, wherein the polyhydric alcohol contains a polyphenol and contains 0.05 to 4% by weight of the polyphenol with respect to the entire xyloglucan-containing composition.   The xyloglucan-containing composition according to any one of claims 1 to 4, which is for adhering to the skin or mucous membrane.   It is a sheet form, The xyloglucan containing composition in any one of Claims 1-5.   A xyloglucan-containing patch sheet comprising the xyloglucan-containing composition according to claim 6 and a sheet-like base material laminated.   It is characterized in that water, xyloglucan, sucrose, and polyhydric alcohol are mixed so that the pH is 3 or more to obtain a sol-like mixed solution, and the resulting mixed solution is gelled to form. A method for producing a xyloglucan-containing gel molded article.   The method for producing a xyloglucan-containing gel molded product according to claim 8, wherein the mixed solution is prepared by mixing the water, the mixed solution of the xyloglucan and the sucrose, and the polyhydric alcohol.   The method for producing a xyloglucan-containing gel molded article according to claim 8 or 9, wherein the mixed solution is gelled while adhering to an adherend region.