JP2012250938A - Method for controlling linepithema humile, controlling agent therefor and method for producing the same - Google Patents
Method for controlling linepithema humile, controlling agent therefor and method for producing the same Download PDFInfo
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Abstract
Description
本発明は、アルゼンチンアリの防除方法に関する。より詳細には、アルゼンチンアリの巣仲間認識フェロモンを、特定の割合で用いるアルゼンチンアリの防除方法、防除剤およびその製造方法に関する。 The present invention relates to a method for controlling Argentine ants. More specifically, the present invention relates to an Argentine ant pest control method, a control agent, and a method for producing the same using an Argentine ant nest companion pheromone in a specific ratio.
アルゼンチンアリ（学名：Linepithema humile）は、その名の通り、アルゼンチンなど南米原産のアリである。アルゼンチンアリは、貿易などの人間活動、物資の流通などによって、北米、ヨーロッパ、アフリカ、オーストラリアなどに分布が拡大している。また、１９９０年代には、日本にも侵入しており、兵庫県、山口県、愛知県、神奈川県、岐阜県などで分布が確認され、特定外来生物に指定されている。 Argentine ants (scientific name: Linepithema humile) are ants native to South America such as Argentina. Argentine ants are spreading to North America, Europe, Africa, Australia, and other areas due to human activities such as trade and the distribution of goods. In the 1990s, it also invaded Japan, and its distribution was confirmed in Hyogo, Yamaguchi, Aichi, Kanagawa, Gifu, etc. and designated as a specific alien organism.
アルゼンチンアリは、攻撃性や繁殖力が強く、近年、アルゼンチンアリによる被害が報告されている。その被害としては、「侵略アリとしての生態系への被害（在来種アリ類の駆逐）」、「不快害虫としての被害」および「農業害虫としての被害」の３つが挙げられる。 Argentine ants have strong aggression and fertility, and in recent years, damage caused by Argentine ants has been reported. There are three types of damage: “damage to ecosystems as invading ants (destroy of native ants)”, “damage as unpleasant pests”, and “damage as agricultural pests”.
従来、害虫の駆除は、毒物を混ぜた餌を摂取させる方法が行われている。しかし、アリのような雑食性の害虫は、食嗜好が一定せず効力を持続させるには限界がある。そこで、近年、フェロモンを用いた害虫の駆除・防除技術（特許文献１〜４）や、所定の成分を含有する防除剤（特許文献５）が開示されている。 Conventionally, pest control has been carried out by ingesting bait mixed with poisons. However, omnivorous pests such as ants have a limited food preference, and there is a limit to sustaining efficacy. In recent years, therefore, pest control / control techniques using pheromones (Patent Documents 1 to 4) and control agents containing predetermined components (Patent Document 5) have been disclosed.
特許文献１には、性フェロモンを長期間、高濃度に保つことにより、害虫の交信を撹乱する効果を高め、防除する方法が記載されている。また、アリの防除を目的として、特許文献２には、警報フェロモンと害虫防除薬剤とを組み合わせたアリ防除剤が記載されている。特許文献３には、アルゼンチンアリの防除や繁殖の抑制を目的として、道標フェロモン（Ｚ−９−ヘキサデセナール）を高濃度で放散してアルゼンチンアリの行動を撹乱する方法が記載されている。しかし、Ｚ−９−ヘキサデセナールは揮発性が高いため、効果が持続しないという欠点が考えられる。特許文献４には、アルゼンチンアリのブルード（卵、幼虫および蛹）認識フェロモンと駆除活性成分とを組み合わせて用いる駆除・防除方法が記載されている。特許文献５には、所定のアルコール、所定の脂肪酸、またはクロチアニジンを有効成分とするアルゼンチンアリ防除剤が記載されている。 Patent Document 1 describes a method of enhancing and controlling the effect of disturbing the communication of insect pests by keeping the sex pheromone at a high concentration for a long period of time. In addition, for the purpose of controlling ants, Patent Document 2 describes an ant control agent that combines an alarm pheromone and a pest control agent. Patent Document 3 describes a method of disturbing the behavior of Argentine ants by releasing a signpost pheromone (Z-9-hexadecenal) at a high concentration for the purpose of controlling Argentine ants and suppressing reproduction. However, since Z-9-hexadecenal is highly volatile, there is a drawback that the effect is not sustained. Patent Document 4 describes a method for controlling and controlling an Argentine ant brood (egg, larva and pupa) recognition pheromone and a controlling active ingredient. Patent Document 5 describes an Argentine ant control agent containing a predetermined alcohol, a predetermined fatty acid, or clothianidin as an active ingredient.
アルゼンチンアリは、大多数の働きアリと女王アリとからなる大規模な巣を形成し、１つの巣には、複数の女王アリが存在し、数百匹の女王アリが存在することもある。アルゼンチンアリは、道標フェロモンやブルード認識フェロモン以外に、巣仲間認識フェロモンを分泌している。巣仲間認識フェロモンにより、同じ巣の個体か異なる巣の個体かを識別している。この巣仲間認識フェロモンを、他の巣のアルゼンチンアリに適用すると、攻撃行動を誘発することが報告されている（非特許文献１）。 Argentine ants form a large nest consisting of a large number of worker ants and queen ants, and one nest contains multiple queen ants and may contain hundreds of queen ants. Argentine ants secrete nest companion pheromones in addition to signpost pheromones and brood recognition pheromones. Nest companion recognition pheromone identifies individuals in the same nest or in different nests. It has been reported that when this nest buddy recognition pheromone is applied to Argentine ants in other nests, it induces aggressive behavior (Non-patent Document 1).
アルゼンチンアリは、上記のように大規模な巣を形成するため、巣全体の駆除は極めて困難であり、従来の駆除技術は、一時的な対処にすぎない。したがって、一刻も早くアルゼンチンアリの分布の拡大を、長期間にわたって効果的に止める（防除する）ことができる技術が求められている。 Argentine ants form a large-scale nest as described above, and thus it is extremely difficult to remove the entire nest, and conventional removal techniques are only temporary measures. Therefore, there is a need for a technique that can effectively stop (control) the expansion of Argentine ants as soon as possible.
本発明の課題は、アルゼンチンアリの分布の拡大を、長期間にわたり効果的に止めることができるアルゼンチンアリ防除剤を提供することである。 An object of the present invention is to provide an Argentine ant control agent that can effectively stop the expansion of Argentine ant distribution over a long period of time.
本発明者らは、上記課題を解決するべく鋭意検討を行った結果、以下の構成からなる解決手段を見出し、本発明を完成するに至った。
（１）アルゼンチンアリが忌避行動を起こすのに十分な量の巣仲間認識フェロモンを、床面または地面に適用することを特徴とする、巣仲間および非巣仲間のアルゼンチンアリの防除方法。
（２）前記巣仲間認識フェロモンの十分な量が、適用面１ｃｍ2あたり５．８μｇ以上である、（１）に記載のアルゼンチンアリの防除方法。
（３）前記巣仲間認識フェロモンの十分な量を担持した担体を、前記床面または地面に適用する、（１）または（２）に記載のアルゼンチンアリの防除方法。
（４）前記担体が、ガラスビーズ、樹脂ビーズ、フィルム、布、パルプ成形体、粉剤およびチョーク剤からなる群より選択される、（３）に記載のアルゼンチンアリの防除方法。
（５）前記巣仲間認識フェロモンが、化学合成によって得られるフェロモンである、（１）〜（４）のいずれかに記載のアルゼンチンアリの防除方法。
（６）前記巣仲間認識フェロモンが、n-tricosane、n-pentacosane、n-hexacosane、n-heptacosane、n-octacosaneおよびn-nonacosaneからなる群より選択される少なくとも１種を含有する、（１）〜（５）のいずれかに記載のアルゼンチンアリの防除方法。
（７）アルゼンチンアリが忌避行動を起こすのに十分な量の巣仲間認識フェロモンを含有することを特徴とする、巣仲間および非巣仲間のアルゼンチンアリ防除剤。
（８）前記巣仲間認識フェロモンが担体に担持されており、前記巣仲間認識フェロモンの担持量が、担体面１ｃｍ2あたり５．８μｇ以上である、（７）に記載のアルゼンチンアリ防除剤。
（９）前記担体が、ガラスビーズ、樹脂ビーズ、フィルム、布、パルプ成形体、粉剤およびチョーク剤からなる群より選択される、（７）または（８）に記載のアルゼンチンアリ防除剤。
（１０）前記巣仲間認識フェロモンが、化学合成によって得られるフェロモンである、（７）〜（９）のいずれかに記載のアルゼンチンアリ防除剤。
（１１）アルゼンチンアリの巣仲間認識フェロモンを、担体面１ｃｍ2あたり５．８μｇ以上となるように、担体に担持させる工程を含むことを特徴とする、巣仲間および非巣仲間のアルゼンチンアリ防除剤の製造方法。
（１２）前記巣仲間認識フェロモンが、化学合成によって得られるフェロモンである、（１１）に記載のアルゼンチンアリ防除剤の製造方法。
As a result of intensive studies to solve the above problems, the present inventors have found a solution means having the following configuration, and have completed the present invention.
(1) A method for controlling nest companions and non-nest companions Argentine ants, characterized by applying a sufficient amount of nest companion pheromones to the floor or the ground to cause Argentine ants to perform repellent behavior.
(2) The method for controlling Argentine ants according to (1), wherein a sufficient amount of the nest companion recognition pheromone is 5.8 μg or more per 1 cm 2 of the application surface.
(3) The method for controlling Argentine ants according to (1) or (2), wherein a carrier carrying a sufficient amount of the nest companion recognition pheromone is applied to the floor or the ground.
(4) The Argentine ant control method according to (3), wherein the carrier is selected from the group consisting of glass beads, resin beads, films, cloths, pulp moldings, powders and chalks.
(5) The method for controlling an Argentine ant according to any one of (1) to (4), wherein the nest-mate recognition pheromone is a pheromone obtained by chemical synthesis.
(6) The nest companion pheromone contains at least one selected from the group consisting of n-tricosane, n-pentacosane, n-hexacosane, n-heptacosane, n-octacosane and n-nonacosane, (1) The control method of the Argentine ant according to any one of to (5).
(7) A nest companion and non-nest companion Argentine ant control agent, comprising an amount of nest companion recognition pheromone sufficient for Argentine ants to perform repellent behavior.
(8) The Argentine ant control agent according to (7), wherein the nest companion recognition pheromone is supported on a carrier, and the amount of the nest companion recognition pheromone supported is 5.8 μg or more per 1 cm 2 of the carrier surface.
(9) The Argentine ant control agent according to (7) or (8), wherein the carrier is selected from the group consisting of glass beads, resin beads, films, cloths, pulp moldings, powders and chalk agents.
(10) The Argentine ant control agent according to any one of (7) to (9), wherein the nest-mate recognition pheromone is a pheromone obtained by chemical synthesis.
(11) Argentine ant nest companion pheromone ant control agent for nest companions and non-nest companions, comprising a step of supporting a carrier on a pheromone recognition pheromone of 5.8 μg per 1 cm 2 of the carrier surface Manufacturing method.
(12) The method for producing an Argentine ant control agent according to (11), wherein the nest-mate recognition pheromone is a pheromone obtained by chemical synthesis.
本発明によれば、アルゼンチンアリにとって本来、巣仲間であるか否かを認識するためのフェロモン（巣仲間認識フェロモン）を、特定量以上用いることによって、アルゼンチンアリに忌避行動を誘発させることができ、優れた忌避効果が得られる。また、巣仲間認識フェロモンは揮発しにくいため、長期間にわたり顕著な忌避効果が得られ、アルゼンチンアリの分布の拡大を、長期間にわたり効果的に止めることができるという効果が得られる。さらに、本発明のアルゼンチンアリの防除方法や防除剤は、アルゼンチンアリのフェロモンを利用したものであり、他の生物や散布・設置など適用した周囲の環境に悪影響を与えることもない。 According to the present invention, Argentine ants can induce repellent behavior by using more than a specific amount of pheromones (nest mate recognition pheromones) for recognizing whether or not they are nest companions. Excellent repellent effect is obtained. Further, since the nest companion recognition pheromone is less likely to volatilize, a significant repelling effect is obtained over a long period of time, and the effect that the expansion of the distribution of Argentine ants can be effectively stopped over a long period of time. Furthermore, the Argentine ant control method and the control agent of the present invention use Argentine ant pheromone, and does not adversely affect other living organisms and the surrounding environment such as application and installation.
本発明に係るアルゼンチンアリの防除方法は、アルゼンチンアリが忌避行動を起こすのに十分な量のアルゼンチンアリの巣仲間認識フェロモンを用いる。なお、本発明において「防除」とは、主として「忌避」を意味し、「忌避行動」とは、防除剤から離れたり戻ったりするような行動であり、驚きのあまりに思わず後ずさりするような行動（すなわち「特異的な忌避行動」）をも包含する。 The method for controlling Argentine ants according to the present invention uses an Argentine ant nest companion pheromone sufficient for Argentine ants to perform repellent behavior. In the present invention, “control” mainly means “repelling”, and “repelling behavior” is an action that moves away from or returns to the control agent, and is an action that is surprisingly unintentional ( That is, it includes “specific repellent behavior”).
アルゼンチンアリの巣仲間認識フェロモンは、アルゼンチンアリの体表に分泌されるフェロモンであり、約３０種の炭化水素類が知られている。これらの炭化水素類は、揮発しにくく水に不溶性の化合物である。本発明に用いられる巣仲間認識フェロモンは、化学合成により得られるものでも、アルゼンチンアリの体表から抽出されるものでも、あるいはこれらの炭化水素類を含むものであれば、アルゼンチンアリ以外のアリの体表から抽出されるものでもよい。生産コストを考慮すると、化学合成で得られるアルゼンチンアリの巣仲間認識フェロモンが好ましい。 Argentine ant nest companion pheromone is a pheromone secreted to the body surface of Argentine ants, and about 30 kinds of hydrocarbons are known. These hydrocarbons are compounds that do not volatilize and are insoluble in water. The nest mate recognition pheromone used in the present invention can be obtained by chemical synthesis, extracted from the body surface of the Argentine ant, or contains any of these hydrocarbons. It may be extracted from the body surface. Considering production costs, an Argentine ant nest companion pheromone obtained by chemical synthesis is preferable.
巣仲間認識フェロモンを、アルゼンチンアリの体表から抽出する方法としては、特に限定されない。例えば、アルゼンチンアリの働きアリ（Ｗｏｒｋｅｒ）を有機溶媒に浸漬し、体表に付着しているフェロモンを濯ぐようにして抽出すればよい。有機溶媒としては、例えば、ヘキサン、酢酸エチル、エーテル、クロロフォルム、ジクロロメタン、エタノールなどが挙げられる。これらの有機溶媒の中でも、ヘキサンが好ましい。ヘキサンを用いると、アリの体液など不要な物質が抽出されにくく、巣仲間認識フェロモンが効率よく抽出される。抽出物中に、巣仲間認識フェロモンが含まれることは、ガスクロマトグラフィー質量分析などの方法により確認（同定）することができる。 The method for extracting the nest companion pheromone from the body surface of the Argentine ant is not particularly limited. For example, an Argentine ant worker (Worker) may be extracted by immersing it in an organic solvent and rinsing pheromone adhering to the body surface. Examples of the organic solvent include hexane, ethyl acetate, ether, chloroform, dichloromethane, ethanol and the like. Of these organic solvents, hexane is preferred. When hexane is used, unnecessary substances such as ant body fluids are not easily extracted, and nest companion pheromones are efficiently extracted. It can be confirmed (identified) by a method such as gas chromatography mass spectrometry that the extract contains a nest companion pheromone.
抽出温度は、通常１５〜３０℃、好ましくは常温（室温）であり、抽出時間は、通常６０〜６００秒、好ましくは２００〜３００秒である。抽出温度を高くしすぎたり、あるいは抽出時間を長くしすぎたりすると、アリの体液など不要な物質が抽出されやすくなり、精製が煩雑になる。 The extraction temperature is usually 15 to 30 ° C., preferably room temperature (room temperature), and the extraction time is usually 60 to 600 seconds, preferably 200 to 300 seconds. If the extraction temperature is set too high or the extraction time is set too long, unnecessary substances such as ant body fluids are easily extracted, and purification becomes complicated.
得られた抽出液は、例えばシリカゲルカラムに通液され、高純度の巣仲間認識フェロモン抽出液が得られる。なお、シリカゲルカラム以外にも、フロリジルカラム、薄層クロマトグライーなどの方法によって精製してもよい。このようにして精製された抽出液には、巣仲間認識フェロモンとして、上記約３０種の炭化水素類のうち少なくとも１種の炭化水素が含まれる。 The obtained extract is passed through, for example, a silica gel column to obtain a high purity nest-mate recognition pheromone extract. In addition to the silica gel column, it may be purified by a method such as a florisil column or thin layer chromatography. The extract thus purified contains at least one hydrocarbon of the above about 30 types of hydrocarbons as a nest companion pheromone.
本発明に用いられる巣仲間認識フェロモンは、上記約３０種の炭化水素類であれば、特に限定されないが、これらの炭化水素類の中でも、n-tricosane、n-pentacosane、n-hexacosane、n-heptacosane、n-octacosaneおよびn-nonacosaneの６種の炭化水素のうち、少なくとも１種を含有していることが好ましい。なお、これら６種の炭化水素は、例えば、クロオオアリの体表抽出物（フェロモン）にも含まれる化合物である。したがって、本発明に用いられる巣仲間認識フェロモンとして、クロオオアリの体表成分（体表抽出物）をそのまま、あるいは６種の炭化水素を単離して用いても差し支えない。 The nest comprehension pheromone used in the present invention is not particularly limited as long as it is the above-mentioned about 30 kinds of hydrocarbons. Among these hydrocarbons, n-tricosane, n-pentacosane, n-hexacosane, n- It is preferable that at least one of the six hydrocarbons of heptacosane, n-octacosane and n-nonacosane is contained. In addition, these 6 types of hydrocarbons are compounds contained also in the body surface extract (pheromone) of the black ant, for example. Therefore, as the nest comprehension pheromone used in the present invention, the body surface component (body surface extract) of the black ant can be used as it is or by isolating six kinds of hydrocarbons.
本発明に係るアルゼンチンアリの防除方法では、アルゼンチンアリの巣仲間認識フェロモンを、アルゼンチンアリが忌避行動を起こすのに十分な量を床面や地面に適用すればよい。床面や地面に適用する際、巣仲間認識フェロモンを直接散布・塗布してもよく、後述の担体に巣仲間認識フェロモンを担持して適用してもよい。「アルゼンチンアリが忌避行動を起こすのに十分な量」とは、適用面１ｃｍ2あたり、好ましくは５．８μｇ以上（５．８μｇ／ｃｍ2以上）、より好ましくは２３μｇ以上（２３μｇ／ｃｍ2以上）、さらに好ましくは１９３μｇ以上（１９３μｇ／ｃｍ2以上）である。適用面１ｃｍ2あたり１．９μｇ未満（１．９μｇ／ｃｍ2未満）の場合、アルゼンチンアリが忌避行動を起こさない割合が高くなるおそれがある。 In the Argentine ant pest control method according to the present invention, the Argentine ant nest companion pheromone may be applied to the floor or the ground in an amount sufficient for the Argentine ant to perform repellent behavior. When applying to the floor or the ground, the nest companion recognition pheromone may be directly sprayed and applied, or the nest companion recognition pheromone may be carried on a carrier described later and applied. The "Argentine ant sufficient amount to cause repellent action", per application face 1 cm 2, preferably more than 5.8Myug is (5.8μg / cm 2 or higher), more preferably more than 23μg (23μg / cm 2 or more ), More preferably 193 μg or more (193 μg / cm 2 or more). In the case of less than 1.9 μg (less than 1.9 μg / cm 2 ) per 1 cm 2 of the application surface, there is a risk that the proportion of Argentine ants that do not cause repelling behavior increases.
本発明のアルゼンチンアリ防除剤は、アルゼンチンアリの巣仲間認識フェロモンを、アルゼンチンアリが忌避行動を起こすのに十分な量を含有する。好ましくは、アルゼンチンアリの巣仲間認識フェロモンは、担体に担持されており、担体面１ｃｍ2あたり、好ましくは５．８μｇ以上（５．８μｇ／ｃｍ2以上）、より好ましくは２３μｇ以上（２３μｇ／ｃｍ2以上）、さらに好ましくは１９３μｇ以上（１９３μｇ／ｃｍ2以上）である。担体面１ｃｍ2あたり１．９μｇ未満（１．９μｇ／ｃｍ2未満）の場合、アルゼンチンアリが忌避行動を起こさない割合が高くなるおそれがある。なお、「適用面」および「担体面」は、面に存在する凹凸などを考慮した表面積ではなく、みかけ上の面積のことをいう。 The Argentine ant control agent of the present invention contains an Argentine ant nest companion recognition pheromone in an amount sufficient for the Argentine ant to perform repellent behavior. Preferably, the Argentine ant nest companion pheromone is supported on a carrier and is preferably 5.8 μg or more (5.8 μg / cm 2 or more), more preferably 23 μg or more (23 μg / cm 2 ) per 1 cm 2 of the carrier surface. 2 or more), more preferably 193 μg or more (193 μg / cm 2 or more). In the case of less than 1.9 μg per 1 cm 2 of the carrier surface (less than 1.9 μg / cm 2 ), there is a risk that the proportion of Argentine ants not causing repelling behavior is increased. The “applied surface” and the “carrier surface” refer to an apparent area, not a surface area that takes into account irregularities present on the surface.
上記の５．８μｇ／ｃｍ2の適用量（担持量）は、例えば、直径約２ｍｍのガラスビーズ、樹脂ビーズなどのような約０．１２６ｃｍ2の表面積を有する担体に担持する場合、約３匹等量に相当する。同様に、２３μｇ／ｃｍ2の適用量（担持量）は約１２匹等量に相当し、１９３μｇ／ｃｍ2の適用量（担持量）は約１００匹等量に相当する。なお、「１匹等量」とは、一般的なアルゼンチンアリの働きアリ（Ｗｏｒｋｅｒ）約１匹分の巣仲間認識フェロモン量（約０．２４μｇ）を示す。 The application amount (supported amount) of the above 5.8 μg / cm 2 is about 3 when supported on a carrier having a surface area of about 0.126 cm 2 such as glass beads, resin beads and the like having a diameter of about 2 mm. Corresponds to an equal amount. Similarly, a dosage of 23μg / cm 2 (supported amount) is equivalent to approximately 12 animals equivalent, a dosage of 193μg / cm 2 (supported amount) corresponds to approximately 100 animals equivalents. The “one equivalent” means the amount of nest companion recognition pheromone (about 0.24 μg) for a general Argentine ant worker ant (Worker).
担体への担持量を特定する方法は、特に限定されず、例えば、担体に担持されている巣仲間認識フェロモン総量を測定し、担持された担体面の面積から１ｃｍ2あたりの担持量を求めればよい。なお、担体に均一に担持されている場合は、必ずしも総量を測定する必要はなく、例えば、担体の任意の範囲（面積）における巣仲間認識フェロモン量を測定し、その任意の範囲（面積）から１ｃｍ2あたりの担持量を求めればよい。巣仲間認識フェロモンを地面など土壌に直接適用した場合などは、土壌に浸潤する分を考慮して、１ｃｍ2あたりの適用量を求める必要がある。なお、巣仲間認識フェロモンの測定は、ガスクロマトグラフィー質量分析計などを用いて行えばよい。 The method for specifying the amount carried on the carrier is not particularly limited. For example, if the total amount of nest comprehension pheromone carried on the carrier is measured, and the amount carried per cm 2 is obtained from the area of the carried carrier surface Good. In addition, when it is uniformly supported by the carrier, it is not always necessary to measure the total amount, for example, by measuring the nest companion recognition pheromone amount in an arbitrary range (area) of the carrier, and from the arbitrary range (area) What is necessary is just to obtain | require the load per cm < 2 >. When the nest companion recognition pheromone is directly applied to the soil such as the ground, it is necessary to obtain an application amount per 1 cm 2 in consideration of the infiltration into the soil. The measurement of the nest companion pheromone may be performed using a gas chromatography mass spectrometer or the like.
本発明に用いられる担体は、巣仲間認識フェロモンを担持し得るものであれば、特に限定されない。担体としては、例えば、ガラスビーズ、樹脂ビーズ、フィルム、布、パルプ成形体、粉剤、チョーク剤などが挙げられる。本明細書において「担持」とは、担体に、巣仲間認識フェロモンが塗布、固着、含浸など任意の状態で付着していることをいう。また、担体が、板状の場合、「担体面」は、その使用態様によって両面または片面のいずれもあり得る。例えば、布やパルプ成形体などのように、巣仲間認識フェロモンを含浸させて用いる場合、あるいは樹脂に練り込んで板状に加工する場合は、通常両面を担体面とし、板状物の片面のみに、巣仲間認識フェロモン塗布する場合は、片面（塗布面）を担体面とする。また、エアゾルなどの溶液に溶解・分散させた防除剤を担体に塗布してもよい。 The carrier used in the present invention is not particularly limited as long as it can carry the nest companion recognition pheromone. Examples of the carrier include glass beads, resin beads, films, cloths, pulp molded products, powders, chalk agents, and the like. In this specification, “supporting” means that the nest member recognition pheromone adheres to the carrier in any state such as application, fixation, or impregnation. Further, when the carrier is plate-like, the “carrier surface” may be either double-sided or single-sided depending on how it is used. For example, when impregnated with nest companion pheromone, such as cloth or pulp molding, or when kneaded into resin and processed into a plate shape, usually both sides are used as carrier surfaces and only one side of the plate-like material In addition, when the nest companion recognition pheromone is applied, one surface (application surface) is set as the carrier surface. Moreover, you may apply | coat the control agent melt | dissolved and disperse | distributed to solutions, such as aerosol, to a support | carrier.
例えば、担体の原料として樹脂を用いる場合、ビーズ状やフィルム状に成形した後、その表面に巣仲間認識フェロモンを塗布してもよく、溶融した樹脂に、最終的に得られる形状の表面積から求められる量の巣仲間認識フェロモンを添加して、ビーズ状やフィルム状、その他任意の形状に成形してもよい。この場合、巣仲間認識フェロモンが樹脂に練り込まれており、表面に塗布するよりも効果の持続が期待できる。また、巣仲間認識フェロモンを含む溶液などを、ビーズやカプセルに内包してもよい。内包する場合、ビーズやカプセルの表面積から求められる量の巣仲間認識フェロモンを用いればよい。布（不織布（フェルトなど）や織布）、パルプ成形体などの繊維集合体を用いる場合、このような繊維集合体に巣仲間認識フェロモンを含浸させてもよい。また、杭や柵のような用途で用いる場合、金属や木を、柱状や錘状に加工して用いるが、この場合「担体面」とは、地上に露出している部分を示す。 For example, when a resin is used as a raw material for the carrier, a nest companion pheromone may be applied to the surface of the resin after it is molded into a bead or film, and the molten resin is obtained from the surface area of the final shape. An amount of nest companion recognition pheromone may be added to form a bead shape, a film shape, or any other shape. In this case, the nest companion recognition pheromone is kneaded into the resin, and the effect can be expected to last longer than when applied to the surface. Further, a solution containing a nest companion recognition pheromone or the like may be included in beads or a capsule. When encapsulating, the nest companion pheromone in an amount determined from the surface area of the beads or capsules may be used. When using a fiber assembly such as a cloth (nonwoven fabric (felt) or woven fabric) or a pulp molding, such a fiber assembly may be impregnated with a nest companion recognition pheromone. Further, when used in applications such as piles and fences, metal and wood are used after being processed into a columnar shape or a weight shape. In this case, the “carrier surface” indicates a portion exposed to the ground.
本発明に用いられる担体は、粉体であってもよい。担体として粉体を用いる場合、巣仲間認識フェロモンは、粉体の比表面積１ｃｍ2／ｇあたり上述の担持量で用いればよい。粉体としては賦形剤が用いられ、例えば、澱粉、炭酸カルシウム、タルク粉、微粉末クレイ、セラミック粉末、微粉末シリカおよびこれらの混合物が挙げられるが、これらに限定されない。これらの粉体と巣仲間認識フェロモンとを混合して粉剤の形態で用いてもよく、混合後、造粒して顆粒の形態や打錠して錠剤の形態で用いてもよい。 The carrier used in the present invention may be a powder. When powder is used as the carrier, the nest companion pheromone may be used in the above-mentioned loading amount per 1 cm 2 / g of the specific surface area of the powder. Excipients are used as the powder, and examples thereof include, but are not limited to, starch, calcium carbonate, talc powder, fine powder clay, ceramic powder, fine powder silica, and mixtures thereof. These powders and nest companion pheromone may be mixed and used in the form of a powder, or after mixing, granulated and granulated or tableted and used in the form of a tablet.
さらに、本発明のアルゼンチンアリ防除剤は、本発明の効果を阻害しない範囲で、従来知られている忌避行動を誘発する成分を併用してもよい。このような成分としては、例えば、アルゼンチンアリの警報フェロモン（抽出物または化学合成物）などが挙げられる。特に、アルゼンチンアリの巣仲間認識フェロモンと警報フェロモンとを組み合わせることによって、より優れた効果が期待できる。 Furthermore, the Argentine ant control agent of this invention may use together the component which induces the repellent behavior known conventionally in the range which does not inhibit the effect of this invention. Examples of such components include Argentine ant alarm pheromones (extracts or chemical compounds). In particular, by combining an Argentine ant nest companion pheromone and an alarm pheromone, a more excellent effect can be expected.
本発明のアルゼンチンアリ防除剤は、床面または地面に散布または設置して用いる。本発明のアルゼンチンアリ防除剤に接触したアルゼンチンアリは、防除剤に近づかなかったり、引き返したり、戻ったり、あるいは後ずさりなどの忌避行動を示し、防除剤を超えて前進しようとしない。その結果、アルゼンチンアリの分布の拡大を防止することができる。特に、アルゼンチンアリの巣の入り口を囲むように防除剤を散布、あるいはリング状に加工したフィルム形態や布形態の防除剤を、入り口を囲むように設置することによって、アルゼンチンアリが巣の周囲に閉じ込められ、より効果的にアルゼンチンアリの分布の拡大を防止することができる。アルゼンチンアリの巣仲間認識フェロモンは、揮発しにくく適用した場所に長時間留まるため、長時間効果が持続する。また、アルゼンチンアリのフェロモンを利用したものであり、他の生物や適用した周囲の環境に悪影響を与えることもなく、安全性にも優れる。さらに、巣仲間認識フェロモンとして、アルゼンチンアリの抽出物を用いる場合、抽出に用いたアルゼンチンアリと異なる巣仲間（非巣仲間）のアルゼンチンアリに適用した場合、より顕著な忌避行動を示し、非常に有用である。 The Argentine ant control agent of the present invention is used by being applied to the floor or ground. Argentine ants that have come into contact with the Argentine ant control agent of the present invention show repellent behavior such as not approaching, turning back, returning, or going back and do not attempt to advance beyond the control agent. As a result, the expansion of Argentine ant distribution can be prevented. In particular, Argentine ants are placed around the nest by spraying a control agent around the entrance of the Argentine ant's nest, or by installing a film or cloth shaped control agent processed into a ring shape so as to surround the entrance. Confined and can prevent the expansion of Argentine ant distribution more effectively. Argentine ant nest companion pheromone is resistant to volatilization and stays in the place where it is applied for a long time, so the effect lasts for a long time. In addition, it uses Argentine ant pheromone, and does not adversely affect other organisms and the surrounding environment to which it is applied, and is excellent in safety. Furthermore, when using an extract of Argentine ants as a nest companion pheromone, when applied to Argentine ants of non-nest companions (non-nest companions) different from the Argentine ants used for extraction, it shows more remarkable repellent behavior, Useful.
本発明は上述するように揮発性の低い体表フェロモンを忌避に十分な高濃度に適用面または担体に塗布・含浸・練込をすると、巣仲間または非巣仲間アルゼンチンアリが後ずさりのような従来技術では見られなかった強い忌避行動を含むアルゼンチンアリの防除法または防除剤に関するものである。上記非特許文献１の３６５ページの“Extraction and application of whole profile cuticular hydrocarbon"および３６７ページのＦｉｇｕｒｅ２に記載されているように、この実験は、６００匹から抽出した体表フェロモンをヘキサンで抽出溶解した後、それを乾燥させて容器壁面に固化した体性表成分に一匹のアリを接触させ、そのアリの体表に抽出した体表フェロモンを固着させる方法である。Ｆｉｇｕｒｅ２に示されるように、Ｃ37成分は未処理のアリの体表成分（約０．１１ｎｇ）に対して、２倍程度（０．２３ｎｇ）しか付着していない事がわかる。すなわち、自己の体表成分に対してさらに１匹分、計２匹分の体表成分しか無いことがわかる。 In the present invention, as described above, when a surface pheromone having low volatility is applied, impregnated, and kneaded to an application surface or a carrier at a sufficiently high concentration to avoid repelling, a nest companion or a non-nest companion Argentine ant has a conventional backlash. It relates to the control method or control agent of Argentine ants including strong repellent behavior not found in the technology. As described in “Extraction and application of whole profile cuticular hydrocarbon” on page 365 of Non-Patent Document 1 and FIG. 2 on page 367, in this experiment, body surface pheromones extracted from 600 animals were extracted and dissolved with hexane. Thereafter, one ant is brought into contact with the body surface component dried and solidified on the container wall surface, and the extracted body surface pheromone is fixed to the body surface of the ant. As shown in FIG. 2, it can be seen that the C 37 component adheres only about twice (0.23 ng) to the untreated ant body surface component (about 0.11 ng). That is, it can be seen that there is only one body surface component for one animal, that is, a total of two body surface components for its own body surface component.
なお、上記非特許文献１に記載されているＺ−９−ヘキサデセナールは、組成式Ｃ16Ｈ30Ｏで分子量２３４である。一方、本発明の６種のフェロモンは直鎖の飽和炭化水素であって、２３〜２９の炭素数（Ｃ23〜Ｃ29）を有する。 Incidentally, Z-9-hexadecenal, which is described in Non-Patent Document 1 is the molecular weight 234 by the composition formula C 16 H 30 O. On the other hand, the six pheromones of the present invention are straight-chain saturated hydrocarbons and have a carbon number of 23 to 29 (C 23 to C 29 ).
以下、実施例および比較例を挙げて本発明を具体的に説明するが、本発明はこれらの実施例に限定されるものではない。 EXAMPLES Hereinafter, although an Example and a comparative example are given and this invention is demonstrated concretely, this invention is not limited to these Examples.
（調製例１：アルゼンチンアリの巣仲間認識フェロモンの抽出）
ヘキサン２０ｍＬに、アルゼンチンアリの働きアリ１００匹を、室温で５分間浸漬した。次いで、全てのアリを取り出し、抽出液をシリカゲルカラムで精製した。なお、溶離液としてジクロロメタンを用いた。次いで、室温下にて気流濃縮により溶媒を除去し、アルゼンチンアリの働きアリ１００匹分の巣仲間認識フェロモンを得た。巣仲間認識フェロモンであることは、攻撃性評価と化学分析によって同定した。化学分析として採用したガスクロマトグラフィーの分析条件は、インジェクション部を通過後、カラム温度はオーブン温度で６０℃にて１分間維持後、３０℃／分で２１０℃まで、さらに１０℃／分で３５０℃まで上昇させ、３５０℃で１５分間維持する昇温クロマトグラムの条件で分析した。
(Preparation Example 1: Extraction of Argentine Ant Nest Friend Recognition Pheromone)
100 Argentine ant workers were immersed in 20 mL of hexane for 5 minutes at room temperature. Then, all ants were taken out and the extract was purified with a silica gel column. Dichloromethane was used as the eluent. Next, the solvent was removed by airflow concentration at room temperature, and nest companion recognition pheromones for 100 Argentine ant workers were obtained. We identified it as a nest companion pheromone by aggressive evaluation and chemical analysis. The analysis conditions of the gas chromatography adopted as the chemical analysis are as follows. After passing through the injection section, the column temperature is maintained at 60 ° C. for 1 minute at the oven temperature, then up to 210 ° C. at 30 ° C./minute, and 350 The analysis was performed under the condition of a temperature rising chromatogram that was raised to 50 ° C. and maintained at 350 ° C. for 15 minutes.
（調整例２：クロオオアリの体表成分の抽出）
ヘキサン２０ｍＬに、クロオオアリの働きアリ６匹を、室温で５分間浸漬した。次いで、全てのアリを取り出し、抽出液をシリカゲルカラムで精製した。なお、溶離液としてヘキサンとジクロロメタンとの混合溶媒を用いた。次いで、室温下にて気流濃縮により溶媒を除去し、クロオオアリの働きアリ６匹分の体表成分を得た。なお、クロオオアリから抽出される体表成分を分析すると、アルゼンチンアリの巣仲間認識フェロモンと共通する５種の成分（炭化水素）が、クロオオアリ１匹あたりアルゼンチンアリの約１００倍含まれることがわかった。
(Adjustment example 2: Extraction of body surface components of the black ant)
Six 20-year-old worker ants were immersed in 20 mL of hexane at room temperature for 5 minutes. Then, all ants were taken out and the extract was purified with a silica gel column. A mixed solvent of hexane and dichloromethane was used as the eluent. Subsequently, the solvent was removed by airflow concentration at room temperature, and body surface components for 6 worker ants of the black ant were obtained. In addition, when analyzing the body surface components extracted from the black ants, it was found that five kinds of components (hydrocarbons) common to the Argentine ant nest companion pheromone are contained about 100 times as much as the Argentine ants per black ant. .
（非巣仲間アルゼンチンアリにおける忌避行動の検証）
（実施例１Ａ）
調製例１で得られたアルゼンチンアリ１００匹分の巣仲間認識フェロモンを、直径約２ｍｍのガラスビーズ（柄付き）に塗布し（ビーズ表面１ｃｍ2あたり１９１μｇ塗布）、アルゼンチンアリ防除剤を得た。次いで、抽出に用いたアルゼンチンアリと異なる巣のアルゼンチンアリ（非巣仲間アルゼンチンアリ）を５匹採取し、その中の１匹をシャーレに入れた。次いで、シャーレに入れたアルゼンチンアリにガラスビーズを近づけ、ガラスビーズに触れたアルゼンチンアリの行動を観察した。次いで、別の１匹をシャーレに入れ、同様の手順で行動を観察した。この観察を繰り返し行った。結果を表１に示す。
(Verification of avoidance behavior in non-nestled Argentine ants)
Example 1A
The nest buddy recognition pheromone for 100 Argentine ants obtained in Preparation Example 1 was applied to glass beads (with a handle) having a diameter of about 2 mm (191 μg applied per 1 cm 2 of the bead surface) to obtain an Argentine ant control agent. Next, five Argentine ants (non-nested Argentine ants) in a nest different from the Argentine ants used for extraction were collected, and one of them was placed in a petri dish. Next, glass beads were brought close to the Argentine ants placed in the petri dish, and the behavior of Argentine ants touching the glass beads was observed. Next, another animal was placed in a petri dish, and behavior was observed in the same procedure. This observation was repeated. The results are shown in Table 1.
（実施例１Ｂ〜１Ｆ）
調製例１で得られたアルゼンチンアリ１００匹分の巣仲間認識フェロモン（１００匹等量）を、ヘキサンを用いて２倍希釈（５０匹等量）、４倍希釈（２５匹等量）、８倍希釈（１２．５匹等量）、１６倍希釈（６．３匹等量）および３２倍希釈（３．１匹等量）したものを用いた以外は、実施例１Ａと同様の手順で、ガラスビーズに触れたアルゼンチンアリの行動を観察した。結果を表１に示す。
(Examples 1B to 1F)
Nest mate recognition pheromone (100 equivalents) for 100 Argentine ants obtained in Preparation Example 1 is diluted 2-fold (50 equivalents), 4-fold dilution (25 equivalents) with hexane, 8 A procedure similar to that of Example 1A was used, except that one-fold diluted (12.5 equivalent), 16-fold diluted (6.3 equivalent), and 32-fold diluted (3.1 equivalent) were used. The behavior of Argentine ants touching the glass beads was observed. The results are shown in Table 1.
（比較例１Ａ〜１Ｄ）
調製例１で得られたアルゼンチンアリ１００匹分の巣仲間認識フェロモンを、ヘキサンを用いて１２８倍希釈（０．８匹等量）、２５６倍希釈（０．４匹等量）、および５１２倍希釈（０．２匹等量）したものを用いた以外は、実施例１Ａと同様の手順で、ガラスビーズに触れたアルゼンチンアリの行動を観察した。また、対照実験として、巣仲間認識フェロモンを用いない（０匹等量）場合を比較例１Ｄとした。結果を表１に示す。表中の「通常の忌避行動」とは、ガラスビーズを避けて近づかなかったり、引き返したり、戻ったりするような行動であり、「特異的な忌避行動」とは、驚きのあまりに思わず後ずさりするような行動である。
(Comparative Examples 1A to 1D)
Nest-mate recognition pheromone for 100 Argentine ants obtained in Preparation Example 1 is diluted 128-fold (0.8 equivalent), 256-fold (0.4 equivalent), and 512-fold with hexane. The behavior of Argentine ants touching the glass beads was observed in the same procedure as in Example 1A, except that diluted (0.2 equivalent) was used. Further, as a control experiment, the case where no nest companion recognition pheromone was used (equal to 0 animals) was defined as Comparative Example 1D. The results are shown in Table 1. “Normal repellent behavior” in the table refers to behavior that keeps away from glass beads, turns back, and returns, and “specific repellent behavior” seems to go back without being surprised too much. It is an action.
表１に示すように、巣仲間認識フェロモンを、６．０〜１９１μｇ／ｃｍ2の割合（ガラスビーズへの塗布量：３．１〜１００匹等量）で塗布したガラスビーズを近づけた場合（実施例１Ａ〜１Ｆ）、ほぼ全てのアルゼンチンアリが、通常または特異的な忌避行動を示すことがわかる。特に、実施例１Ａでは、５匹中２匹のアルゼンチンアリが、特異的な忌避行動を示すことがわかる。 As shown in Table 1, when glass beads applied with nest companion recognition pheromones at a rate of 6.0 to 191 μg / cm 2 (applied amount to glass beads: 3.1 to 100 equivalents) are brought close ( Examples 1A-1F), it can be seen that almost all Argentine ants show normal or specific repellent behavior. In particular, in Example 1A, it can be seen that 2 out of 5 Argentine ants exhibit specific repellent behavior.
一方、巣仲間認識フェロモンが、１．５μｇ／ｃｍ2未満（ガラスビーズへの塗布量：０．８匹等量未満）の場合（比較例１Ａ〜１Ｄ）、ガラスビーズを近づけても、全く忌避行動を示さないことがわかる。 On the other hand, when the nest companion recognition pheromone is less than 1.5 μg / cm 2 (applied amount to glass beads: less than 0.8 equivalent), even if the glass beads are brought close together, it is completely avoided. It can be seen that no action is taken.
（巣仲間アルゼンチンアリにおける忌避行動の検証）
（実施例２Ａ）
非巣仲間アルゼンチンアリの代わりに、抽出に用いたアルゼンチンアリと同じ巣のアルゼンチンアリ（巣仲間アルゼンチンアリ）を用いた以外は、実施例１Ａと同様の手順で、ガラスビーズに触れたアルゼンチンアリの行動を観察した。結果を表２に示す。
(Verification of repellent behavior in nest companion Argentine ants)
(Example 2A)
The Argentine ant touched the glass beads in the same procedure as in Example 1A, except that the Argentine ant of the same nest as the Argentine ant used for extraction (Nest bud Argentine ant) was used instead of the non-nested Argentine ant. Observed behavior. The results are shown in Table 2.
（実施例２Ｂおよび２Ｃ）
調製例１で得られたアルゼンチンアリ１００匹分の巣仲間認識フェロモン（１００匹等量）を、ヘキサンを用いて２倍希釈（５０匹等量）および４倍希釈（２５匹等量）したものを用いた以外は、実施例２Ａと同様の手順で、ガラスビーズに触れたアルゼンチンアリの行動を観察した。結果を表２に示す。
(Examples 2B and 2C)
Nest mate recognition pheromone (100 equivalents) for 100 Argentine ants obtained in Preparation Example 1 diluted 2 times (50 equivalents) and 4 times (25 equivalents) with hexane The behavior of Argentine ants touching the glass beads was observed in the same procedure as in Example 2A except that was used. The results are shown in Table 2.
（比較例２Ａ〜２Ｅ）
調製例１で得られたアルゼンチンアリ１００匹分の巣仲間認識フェロモンを、ヘキサンを用いて６４倍希釈（１．６匹等量）、１２８倍希釈（０．８匹等量）、２５６倍希釈（０．４匹等量）、および５１２倍希釈（０．２匹等量）したものを用いた以外は、実施例２Ａと同様の手順で、ガラスビーズに触れたアルゼンチンアリの行動を観察した。また、対照実験として、巣仲間認識フェロモンを用いない（０匹等量）場合を比較例２Ｅとした。結果を表２に示す。
(Comparative Examples 2A to 2E)
Nest mate recognition pheromone for 100 Argentine ants obtained in Preparation Example 1 is diluted 64 times (1.6 equivalents), 128 times (0.8 equivalents), 256 times diluted with hexane. The behavior of Argentine ants touching the glass beads was observed in the same procedure as Example 2A, except that (0.4 equivalent) and 512-fold diluted (0.2 equivalent) were used. . In addition, as a control experiment, a case where no nest companion recognition pheromone was used (equal to 0 animals) was defined as Comparative Example 2E. The results are shown in Table 2.
表２に示すように、巣仲間認識フェロモンを、４７．８〜１９１μｇ／ｃｍ2の割合（ガラスビーズへの塗布量：２５〜１００匹等量）で塗布したガラスビーズを近づけた場合（実施例２Ａ〜２Ｃ）、巣仲間であるにもかかわらず、忌避行動を示すことがわかる。 As shown in Table 2, when glass beads applied with nest companion recognition pheromone at a rate of 47.8 to 191 μg / cm 2 (applied amount to glass beads: 25 to 100 equivalents) were brought close (Examples) 2A to 2C), it can be seen that although it is a nest companion, it exhibits repellent behavior.
一方、巣仲間認識フェロモンが、２．９μｇ／ｃｍ2未満（ガラスビーズへの塗布量：１．６匹等量未満）の場合（比較例２Ａ〜２Ｅ）、ガラスビーズを近づけても、全く忌避行動を示さないことがわかる。 On the other hand, when the nest companion recognition pheromone is less than 2.9 μg / cm 2 (applied amount to glass beads: less than 1.6 equivalents) (Comparative Examples 2A to 2E), even if the glass beads are brought close together, it is completely avoided It can be seen that no action is taken.
（クロオオアリの体表成分を用いた場合における忌避行動の検証）
（実施例３Ａ）
アルゼンチンアリの巣仲間認識フェロモンとして、調製例２で得られたクロオオアリ６匹分（アルゼンチンアリ６００匹等量に相当）の体表成分を用いた以外は、実施例１Ａと同様の手順で、ガラスビーズに触れたアルゼンチンアリの行動を観察した。結果を表３に示す。
(Verification of repellent behavior when using the surface component of the black ant)
(Example 3A)
As the Argentine ant nest companion pheromone, the same procedure as in Example 1A was used, except that the body surface components of 6 crocodiles obtained in Preparation Example 2 (corresponding to the equivalent of 600 Argentine ants) were used. We observed the behavior of Argentine ants who touched the beads. The results are shown in Table 3.
（実施例３Ｂ〜３Ｇ）
クロオオアリ６匹分の体表成分を、ヘキサンを用いて２倍希釈（３匹等量（アルゼンチンアリ３００匹等量に相当））、４倍希釈（３匹等量（アルゼンチンアリ１５０匹等量に相当））、８倍希釈（０．８匹等量（アルゼンチンアリ８０匹等量に相当））、１６倍希釈（０．４匹等量（アルゼンチンアリ４０匹等量に相当））、３２倍希釈（０．２匹等量（アルゼンチンアリ２０匹等量に相当））および６４倍希釈（０．１匹等量（アルゼンチンアリ１０匹等量に相当））したものを用いた以外は、実施例３Ａと同様の手順で、ガラスビーズに触れたアルゼンチンアリの行動を観察した。結果を表３に示す。
(Examples 3B to 3G)
The body surface components of 6 black ants are diluted 2 times with hexane (equal to 3 animals (corresponding to 300 equivalents of Argentine ants)), 4 times diluted (equal to 3 animals (equal to 150 Argentine ants) Equivalent)), 8 times diluted (equivalent to 0.8 animals (equivalent to 80 equivalents of Argentine ants)), 16 times diluted (equivalent to 0.4 animals (equivalent to 40 equivalents of Argentine ants)), 32 times Except for using diluted (equal to 0.2 animals (equivalent to 20 equivalents of Argentine ants)) and 64-fold dilution (equivalent to 0.1 animals (equivalent to 10 equivalents of Argentine ants)) The behavior of Argentine ants touching the glass beads was observed in the same procedure as in Example 3A. The results are shown in Table 3.
（比較例３Ａ）
クロオオアリの体表成分を用いない（０匹等量）以外は、実施例３Ａと同様の手順で、ガラスビーズに触れたアルゼンチンアリの行動を観察した（対照実験）。結果を表３に示す。
(Comparative Example 3A)
The behavior of Argentine ants touching the glass beads was observed in the same procedure as in Example 3A, except that the body surface component of the black ant was not used (equal to 0 animals) (control experiment). The results are shown in Table 3.
表３に示すように、クロオオアリの体表成分を、１８〜１１４６μｇ／ｃｍ2の割合（ガラスビーズへの塗布量：０．１〜６匹等量）で塗布したガラスビーズを近づけた場合（実施例３Ａ〜３Ｇ）、ほぼ全てのアルゼンチンアリが、通常または特異な忌避行動を示すことがわかる。 As shown in Table 3, when the glass bead coated with the body surface component of the blue ant was applied at a rate of 18 to 1146 μg / cm 2 (applied amount to glass beads: 0.1 to 6 equivalence) (implementation) Examples 3A-3G), it can be seen that almost all Argentine ants exhibit normal or unusual repellent behavior.
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| JP2011125363A Active JP5835703B2 (en) | 2011-06-03 | 2011-06-03 | Argentine ant control method, control agent, and production method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2015214507A (en) * | 2014-05-09 | 2015-12-03 | アース製薬株式会社 | Bedbug spread prevention method |
| WO2023074396A1 (en) | 2021-10-29 | 2023-05-04 | 信越化学工業株式会社 | Ant control agent and ant control method |
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| JPH04230604A (en) * | 1990-12-28 | 1992-08-19 | Earth Chem Corp Ltd | Insect pest controlling agent |
| JP2005263651A (en) * | 2004-03-17 | 2005-09-29 | Shin Etsu Chem Co Ltd | Behavior disrupter and behavior disrupting method of linepithema humile |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2015214507A (en) * | 2014-05-09 | 2015-12-03 | アース製薬株式会社 | Bedbug spread prevention method |
| WO2023074396A1 (en) | 2021-10-29 | 2023-05-04 | 信越化学工業株式会社 | Ant control agent and ant control method |
| KR20240090414A (en) | 2021-10-29 | 2024-06-21 | 신에쓰 가가꾸 고교 가부시끼가이샤 | Ant control agents and methods |
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| JP5835703B2 (en) | 2015-12-24 |
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