JP2012236803A - Method for producing polyamine composition from plant - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a method for producing a polyamine composition which is originated from a plant material of low salt concentration, in good production efficiency.SOLUTION: This method for producing the polyamine composition includes: (1) a step of treating a plant and/or processed plant product with water; and (2) a step of separating a liquid fraction and collecting it.

Description

The present invention relates to a method for producing a polyamine composition from a plant.

Polyamine is a general term for aliphatic hydrocarbons having two or more primary amino groups, and is a natural product existing in the living body, and more than 20 types of polyamines have been found. In recent years, polyamines have become more important as their various physiological activities have been elucidated. The main physiological functions of polyamines are as follows: (1) Nucleic acid stabilization and structural changes by interaction with nucleic acids (2) Promoting action on various nucleic acid synthesis systems (3) Activation of protein synthesis systems (4) Cell membrane Stabilization and enhancement of membrane permeability of substances (5) elimination of active oxygen (6) promotion of cell proliferation (7) antiallergic action is known. Polyamines or polyamine compositions are being used for health foods, cosmetics, foods, and pharmaceutical applications.

As a production method of a polyamine or a polyamine composition that can be used industrially, there are a method of extracting and adjusting from a fish larva (Patent Document 1), a method of separating and recovering from milk or milk material (Patent Document 2, Patent Document 3). , Methods for adjusting yeast cells or yeast cultures under acidic conditions (Patent Documents 4 and 5) are disclosed. Moreover, as a preparation method of the polyamine composition from a plant material, it is a method of extracting a plant material under acidic conditions (Patent Document 6, Patent Document 7), such as sodium chloride, magnesium chloride, calcium chloride, etc. A method for producing a polyamine extract by adding a salt solution (Patent Document 8) has been studied. Further, Patent Document 8 discloses a method of performing water extraction at a high temperature (80 to 100 ° C.) for 5 to 10 minutes, but it is concluded that polyamine is not extracted by water extraction.

JP-A-8-238094 Japanese Patent Laid-Open No. 2001-8663 Japanese Patent Laid-Open No. 2001-95483 Japanese Patent Laid-Open No. 10-52291 JP 2000-245493 A JP-A-10-101624 JP 2007-291027 A JP 2010-263816 A

Plant Cell Physiol. 43 (2), 196-206, 2002 J. et al. Nutr. Biochem. 4, 66-70, 1993 Biosci. Biotech. Biochem. , 61 (9), 1582-1584, 1997

Conventionally, when producing a polyamine or a polyamine composition from a plant material, the plant material has been produced through a step of subjecting the plant material to an extraction step under a strong acid condition having a pH of 2 or less, and neutralizing the extract with an alkaline solution. However, since a large amount of an acid solution for strong acid conditions and an alkaline solution for neutralization are used, the salt concentration contained in the final polyamine composition inevitably increases, and health foods, cosmetics, foods, It was a problem when blended into pharmaceuticals.

In the method of producing a polyamine extract by adding a salt solution such as sodium chloride, magnesium chloride, calcium chloride to a plant material (Patent Document 8), since the salt contained in the final composition increases, Problems arise when blended into cosmetics, foods, pharmaceuticals, etc.

Therefore, there has been a demand for a method for producing a polyamine composition that is a plant material-derived polyamine composition that does not contain a salt derived from the production process and that has good production efficiency.

In addition, the extraction process under strong acid conditions corrodes a stainless steel tank that is widely used in production, making mass production difficult. For these reasons, there has been a demand for a method for producing a polyamine composition that does not use an acid solution.

As a result of earnest examination of a method for producing a polyamine composition that does not produce a salt derived from the production process and has high production efficiency and does not use an acid solution, the present inventors have conducted extraction with water. A method for producing a polyamine composition by a process has been found and the present invention has been achieved.

That is, this invention provides the manufacturing method and polyamine composition of the following polyamine compositions.
[1]
(1) The process of processing a plant and / or a plant processed material with water, and (2) The process of isolate | separating and extract | collecting a liquid fraction, The manufacturing method of the polyamine composition characterized by the above-mentioned.
[2]
The method for producing a polyamine composition according to [1], wherein the step of treating the plant and / or the processed plant product with water is performed at a water temperature of 10 ° C to 60 ° C for 30 minutes to 24 hours.
[3]
The method for producing a polyamine composition according to [2], wherein the step of treating the plant and / or the processed plant product with water is performed at a water temperature of 15 ° C. to 45 ° C. for 30 minutes to 16 hours.
[4]
The method for producing a polyamine composition according to [3], wherein the step of treating the plant and / or the processed plant product with water is performed at a water temperature of 20 ° C. to 30 ° C. for 1 hour to 6 hours.
[5]
Plant and / or plant processed product derived from at least one selected from the group consisting of soybean seed, soybean germ, soybean embryo, wheat seed, wheat germ, wheat embryo, soy milk and okara The method for producing a polyamine composition according to any one of [1] to [4].
[6]
Any of [1] to [5], wherein the plant and / or plant processed product is a plant and / or plant processed product derived from at least one selected from the group consisting of wheat seed, wheat germ and wheat embryo The manufacturing method of the polyamine composition as described in 1 above.
[7]
Polyamine composition is 1,3-diaminopropane, putrescine, cadaverine, cardine, spermidine, homospermidine, aminopropyl cadaverine, theremin, spermine, thermospermine, canabalmine, aminopentylnorspermidine, N, N-bis (aminopropyl) [1] to [6], which is a composition comprising at least one compound selected from the group consisting of cadaverine, homospermine, cardopentamine, homocardopentamine, cardohexamine and homocardohexamine. A method for producing a polyamine composition.
[8]
The method for producing a polyamine composition according to [7], wherein the polyamine composition is a composition comprising at least one compound selected from the group consisting of putrescine, cadaverine, spermidine and spermine.
[9]
A polyamine composition produced from a plant and / or a processed plant product, wherein the polyamine composition does not substantially contain sodium chloride or sodium citrate in the solid content.

According to the present invention, unlike a method for extracting a polyamine composition from a plant material under acidic conditions, a salt is not generated in the extraction step, and a polyamine composition substantially free from a salt derived from the extraction step is obtained. Can do. Furthermore, when producing a polyamine composition from cereals such as wheat, extraction at a high temperature makes it difficult to extract the polyamine composition due to an increase in viscosity due to glutelin or prolamin in the cereal. A high concentration of polyamine can be produced efficiently. That is, the present invention has a remarkable effect that is not possible in the prior art in that a polyamine composition having a high polyamine concentration that does not substantially contain a salt can be produced.

Furthermore, there is no treatment step under strong acid conditions, and the influence of stainless steel corrosion due to acid is eliminated. As a result, it is possible to use a large-capacity stainless steel tank that is widely used in industry, and mass production of polyamine compositions becomes possible. .

In the present invention, “plant” and “processed plant product” refer to a plant body or plant tissue and a product obtained by processing them, and “and / or” includes both or one of them. It means that it is OK.

Various kinds of plants and plant processed products can be used, and are not particularly limited. For example, cucurbitaceae plants, solanaceous plants, gramineous plants, cruciferous plants, legumes, mallows, asteraceae plants. , Red plants, legumes, camellia plants, plant extracts, plant extracts, processed products, and the like. Specifically, sweet potato, tomato, cucumber, pumpkin, melon, watermelon, tobacco, Arabidopsis, pepper, eggplant, bean, taro, spinach, carrot, strawberry, potato, rice, corn, alfalfa, wheat, barley, soybean, rape , Sorghum, eucalyptus, poplar, kenaf, sugarcane, sugar cane, sugar beet, cassava, sago palm, red almond, lily, orchid, carnation, rose, chrysanthemum, petunia, torenia, snapdragon, cyclamen, gypsophila, geranium, sunflower, shiba, cotton, Enokitake, Honshimeji, Matsutake, Shiitake, Mushrooms, Ginseng, Agaricus, Turmeric, Ginseng, Citrus, Banana, Kiwi, Juice, Rice Germ, Wheat Germ, Barley Germ, Soybean Germ, Carrot Kosi germ, milo germ, sunflower germ, soybean extract, wheat extract, germ extract, embryo extract, green tea, black tea, oolong tea, natto, soy milk, such as Ocala, and the like. Preferred are gramineous plants and legumes, and more preferred are gramineous plants.

Although it does not specifically limit as a plant body or plant tissue used for manufacture of a polyamine composition, Preferably it is a plant body or tissue in a seed form or a growth process. Examples of plants or tissues in the form of seeds or in the process of growth include whole trees, flowers, buds, ovary, fruits, leaves, cotyledons, stems, buds, roots, seeds, dried seeds, embryos, germs, roots . Preferred are fruits, leaves, stems, buds, seeds, dried seeds, germs, and embryos, and particularly preferred are seeds, dried seeds, germs, and embryos.

In the present invention, soybean seeds, soybean germs, soybean embryos, wheat seeds, wheat germs, wheat embryos, processed plant products, soy milk, okara, or a combination thereof can be suitably used. More preferably, wheat seed, wheat germ, or wheat embryo can be used.

“Process of treating plant and / or plant processed product with water” refers to a step of immersing the plant and / or plant processed product in water and extracting a composition containing polyamine from the plant and / or plant processed product. . “Water” refers to water to which no acid solution or alkali solution has been added. This step may be left standing or stirred. The treatment time varies depending on the extraction amount, but is preferably 30 minutes or more, more preferably 1 hour or more, and further preferably 3 hours or more. If it is shorter than 30 minutes, a sufficient amount of the polyamine composition may not be extracted. The upper limit is not limited, but it is usually 24 hours or less, preferably 16 hours or less, more preferably 6 hours or less. Even if the treatment is performed for 24 hours or more, the extraction amount of the polyamine composition tends to be saturated, and the amount that can be newly extracted thereafter is considered to be limited.

The water temperature is preferably 10 ° C or higher, more preferably 15 ° C or higher, and still more preferably 20 ° C or higher. When the water temperature is lower than 10 ° C., the solubility of the polyamine composition is lowered, and the production efficiency is remarkably deteriorated. The water temperature is preferably 60 ° C. or lower, more preferably 45 ° C. or lower, and further preferably 30 ° C. or lower. When the polyamine composition is produced from a plant or processed plant, which is a grain such as wheat, particularly when the temperature is 60 ° C. or higher, the viscosity due to gluten increases and the extraction work efficiency decreases, and handling of the produced polyamine composition It is not preferable because the properties deteriorate. Gluten, also called silicon, is a mixture mainly composed of proteins contained in cereals. Examples of proteins included include glutelin and prolamin. Glutelin includes wheat glutenin, rice oryzinin, and the like. Prolamin includes wheat gliadin, corn zein, barley hordein, and the like.

The present invention includes the step of separating and collecting the liquid fraction. This step is a step of treating a plant and / or a processed plant product under water and acidic conditions, and separating and recovering the liquid fraction from plant residue and precipitate by centrifugation and / or filtration. The recovered liquid fraction contains a large amount of polyamine, and becomes a polyamine composition.

The present invention includes a step of adjusting the aqueous solution or polyamine composition used for extraction of the polyamine composition to pH 6.5 to pH 7.5 as necessary. This step can be performed either before or after the step of separating and collecting the liquid fraction, but this step is preferably performed after the step of separating and collecting the liquid fraction.

The “polyamine composition” refers to a composition containing a compound derived from a plant, including a polyamine. The polyamine composition may contain natural components other than polyamines, such as saccharides such as monosaccharides, oligosaccharides and polysaccharides, peptides and proteins. The form of the polyamine composition is provided in the form of an aqueous solution or in the form of a powder. The powder of the polyamine composition is obtained by treating plants and / or plant processed products under water and acidic conditions, and treating the liquid fraction collected by centrifugation and / or filtration separation by spray drying or vacuum freeze drying. Can be obtained.

In the present invention, “polyamine” is a general term for aliphatic hydrocarbons having two or more primary amino groups, and is a natural product that exists universally in the living body, and more than 20 types of polyamines have been found. For example, 1,3-diaminopropane, putrescine, cadaverine, cardine, spermidine, homospermidine, aminopropyl cadaverine, theremin, spermine, thermospermine, canabalmin, aminopentylnorspermidine, N, N-bis (aminopropyl) cadaverine, homo Examples include spermine, cardopentamine, homocardopentamine, cardohexamine, and homocardohexamine. Typical polyamines include putrescine, spermidine and spermine.

Putrescine is one of the typical polyamines, a general natural product that exists universally in living organisms, and is an aliphatic hydrocarbon compound having two primary amino groups. Cadaverine is one of the typical polyamines, a general natural product that exists universally in living organisms, and is an aliphatic hydrocarbon compound having two primary amino groups. Spermidine is one of the typical polyamines, and is a general natural product that exists universally in living organisms, and is an aliphatic hydrocarbon compound having three primary amino groups. Spermine is one of the typical polyamines, and is a general natural product that exists universally in living organisms, and is an aliphatic hydrocarbon compound having four primary amino groups.

If necessary, the polyamine composition may be subjected to desalting treatment or purification treatment by ion exchange method, membrane fractionation method, gel filtration method, electrodialysis method, and by carrying out at least one of these methods. A higher purity polyamine composition can be obtained. For example, as an ion exchange method, a polyamine solution is passed through a column packed with an ion exchange resin to separate the polyamine from impurities such as amino acids, peptides, proteins, and sugars. As the ion exchange resin to be used, the ion exchange group may be a sulfonic acid group, sulfopropyl group, phosphoric acid group, carboxylmethyl group, aminoethyl group, diethylamino group, quaternary aminoethyl group, quaternary ammonium group, etc. Either a cation exchange resin or an anion exchange resin can be used. When a cation exchange resin is used, the polyamine is adsorbed on the cation exchange resin. Therefore, after sufficiently separating non-adsorbed substances, the polyamine is removed with an acidic solution such as sulfuric acid or hydrochloric acid, or a salt solution such as sodium chloride. Elute. When an anion exchange resin is used, the polyamine is not adsorbed on the anion exchange resin, and thus the non-adsorbed fraction containing the polyamine is collected. For example, as a membrane fractionation method, cellulose, cellulose acetate, polysulfone, polyamide, polyacrylonitrile, polytetrafluoroethylene, polyester, polypropylene and the like have a fractional molecular weight in the range of 1000 to 100,000. The polyamine composition is subjected to UF using an ultrafiltration (UF) membrane, and the permeate containing the polyamine is recovered. Further, NF of the polyamine solution is performed using a nanofiltration (NF) membrane having a salt rejection rate of 30 to 80% for desalting. For example, in the gel filtration method, the polyamine composition is neutralized and passed through a column packed with a gel filtration carrier to recover the polyamine by molecular weight fractionation. The gel filtration carrier used is a dextran-based, acrylamide-based, agarose-based, cellulose-based, polyvinyl-based, glass-based, polystyrene-based, or the like with a molecular weight cut-off in the range of 100 to 100,000. For example, in electrodialysis, electrodialysis is performed by alternately supplying a polyamine composition and saline between each membrane partitioned by a cation exchange membrane and an anion exchange membrane. The electrodialysis conditions include an initial current density of 0.5 to 15 A / dm 2 and a voltage of 0.1 to 1.5 V / tank.

In the polyamine composition, if necessary, sucrose fatty acid esters generally used as excipients, emulsifiers such as fatty acid esters, crystalline cellulose, enzymatically degraded dextrin, indigestible dextrin, cluster dextrin, cyclodextrin, Maltooligosaccharide, isomaltooligosaccharide, galactooligosaccharide and the like can be added.

The present invention provides a polyamine composition produced from a plant and / or a plant processed product, which is substantially free from sodium chloride or sodium citrate in the solid content. Sodium chloride and sodium citrate are one type of salt produced by neutralizing a treatment solution that has been made acidic with hydrochloric acid or citric acid in a conventional method for extracting a polyamine composition under acidic conditions. Since this invention does not include the treatment process by acidic conditions, it has the characteristics that salt is not produced | generated substantially and the salt derived from a manufacturing process is not included. The “solid content” is a solute content excluding water in a polyamine composition (solution) that is an aqueous solution, and includes saccharides, peptides, and plant-derived salts in addition to polyamines. When the polyamine composition is a powder, the “solid content” refers to the entire polyamine composition that is a powder, and the salt in the solid content refers to a salt in the powder.

The polyamine composition of the present invention can be used in cosmetics, foods, pharmaceuticals, quasi drugs, animal feeds and the like.

EXAMPLES The present invention will be specifically described below with reference to examples of the present invention, but is not limited thereto.

(Polyamine concentration measurement method)
The polyamine content in the sample was examined by the following method. Polyamines in plants include free polyamines, combined polyamines, and bound polyamines, which can be analyzed (Non-Patent Documents 1-3), but in the present invention, free polyamines were measured.
(1) In a microtube with a screw cap, 20 μl of sample, 360 μl of exchange water, 20 μl of internal standard solution (0.05 nmol / μl 1,7-diamine heptane), 200 μl of saturated sodium carbonate aqueous solution, 200 μl of dansyl chloride / acetone solution ( 10 mg / mL) and mix gently. After closing the tube tightly, heat in a 60 ° C water bath for 1 hour to perform dansylation.
(2) After allowing the tube to cool, 200 μL of an aqueous proline solution (100 mg / mL) is added and mixed. Rewarm in water bath for 30 minutes.
(3) After re-cooling, nitrogen gas is blown to remove acetone, and then 600 μL of toluene is added and mixed vigorously. After centrifuging the tube into two phases, the upper toluene layer is dispensed into 500 μL microtubes. Nitrogen gas is blown onto the collected toluene to completely remove the toluene. 120 μL of methanol is added to the microtube to dissolve the dansylated free polyamine.
(4) The amount of free polyamines of putrescine, spermidine and spermine is analyzed by an internal standard method using high performance liquid chromatography connected with a fluorescence detector (excitation wavelength: 365 nm, emission wavelength: 510 nm). As the HPLC column, μ Bondapak C18 (manufactured by Waters: 0273324, 3.9 × 300 mm, particle diameter 10 μm) is used. The polyamine content in the sample is calculated by obtaining the peak areas of each polyamine and the internal standard from the standard solution and the HPLC chart of the sample, respectively.

(Comparative Example 1) Production method of polyamine composition including a step of treating a plant under strong acid conditions (1) 400 g of wheat germ was added to 1600 g of 1N aqueous HCl solution, and a stirrer (three one motor, The mixture was stirred at 100 rpm for 4 hours using HEIDON.
(2) Next, centrifugation was performed at 8000 rpm for 20 minutes, and the supernatant was recovered.
(3) The pH of the supernatant was adjusted to 7.0 using 1N NaOH aqueous solution.
(4) Centrifugation was further performed at 8000 rpm for 20 minutes, and the resulting supernatant was designated as Extract A.
(5) The polyamine concentration, NaCl concentration, solid content concentration, and viscosity of Extract A were measured. The NaCl concentration was measured using a compact salinity meter C-121 (manufactured by HORIBA), and the solid content concentration was measured using a digital sugar meter APAL-1 (manufactured by AS ONE). The viscosity was measured using a vibration type viscometer VISCOMATE VM-10A (manufactured by CBC).

As a result of Comparative Example 1, the polyamine concentration was 0.0166%, the solid content concentration was 13.8%, the NaCl concentration was 4.00%, the NaCl concentration per solid content was 29.0%, and the viscosity was 8.0. It was 9 mPa · s.
Table 1 shows the results of Comparative Examples 1 and 2 and Examples 1 to 5.

(Examples 1-5) Manufacturing method of polyamine composition including the process of processing a plant only with water (Example 1)
(1) 400 g of wheat germ was added to 1600 g of pure water, and the mixture was stirred at 100 rpm for 30 minutes using a stirrer (three-one motor, manufactured by HEIDON) under the condition of a water temperature of 25 ° C.
(2) Next, the mixture was centrifuged at 8000 rpm for 20 minutes, and the resulting supernatant was used as extract D.
(3) The polyamine concentration, NaCl concentration, solid content concentration, and viscosity of the extract D were measured. The NaCl concentration was measured using a compact salinity meter C-121 (manufactured by HORIBA), and the solid content concentration was measured using a digital sugar meter APAL-1 (manufactured by AS ONE). The viscosity was measured using a vibration type viscometer VISCOMATE VM-10A (manufactured by CBC).

As a result of Example 1, the polyamine concentration was 0.0085%, and the relative polyamine concentration was 51.2% when the polyamine concentration of Comparative Example 1 was 100. The solid content concentration was 8.6%, the NaCl concentration was 0.00%, the NaCl concentration per solid content was 0.00%, and the viscosity was 4.1 mPa · s.

(Example 2)
(1) 400 g of wheat germ was added to 1600 g of pure water, and the mixture was stirred for 1 hour at 100 rpm using a stirrer (Three-One Motor, manufactured by HEIDON) under the condition of a water temperature of 25 ° C.
(2) Next, the mixture was centrifuged at 8000 rpm for 20 minutes, and the resulting supernatant was used as extract D.
(3) The polyamine concentration, NaCl concentration, solid content concentration, and viscosity of the extract D were measured. The NaCl concentration was measured using a compact salinity meter C-121 (manufactured by HORIBA), and the solid content concentration was measured using a digital sugar meter APAL-1 (manufactured by AS ONE). The viscosity was measured using a vibration type viscometer VISCOMATE VM-10A (manufactured by CBC).

As a result of Example 2, the polyamine concentration was 0.0086%, and the relative polyamine concentration was 51.8% when the polyamine concentration of Comparative Example 1 was 100. The solid content concentration was 8.6%, the NaCl concentration was 0.00%, the NaCl concentration per solid content was 0.00%, and the viscosity was 4.1 mPa · s.

(Example 3)
(1) 400 g of wheat germ was added to 1600 g of pure water, and the mixture was stirred at 100 rpm for 4 hours using a stirrer (Three-One Motor, manufactured by HEIDON) under the condition of a water temperature of 25 ° C.
(2) Next, the mixture was centrifuged at 8000 rpm for 20 minutes, and the resulting supernatant was used as Extract B.
(3) The polyamine concentration, NaCl concentration, solid content concentration, and viscosity of Extract B were measured. The NaCl concentration was measured using a compact salinity meter C-121 (manufactured by HORIBA), and the solid content concentration was measured using a digital sugar meter APAL-1 (manufactured by AS ONE). The viscosity was measured using a vibration type viscometer VISCOMATE VM-10A (manufactured by CBC).

As a result of Example 3, the polyamine concentration was 0.0090%, and the relative polyamine concentration was 54.2% when the polyamine concentration of Comparative Example 1 was 100. The solid content concentration was 8.6%, the NaCl concentration was 0.00%, the NaCl concentration per solid content was 0.00%, and the viscosity was 4.9 mPa · s.

Example 4
(1) 400 g of wheat germ was added to 1600 g of pure water, and the mixture was stirred at 100 rpm for 4 hours using a stirrer (three-one motor, manufactured by HEIDON) under the condition of a water temperature of 15 ° C.
(2) Next, centrifugation was performed at 8000 rpm for 20 minutes, and the resulting supernatant was used as extract E.
(3) The polyamine concentration, NaCl concentration, solid content concentration, and viscosity of the extract E were measured. The NaCl concentration was measured using a compact salinity meter C-121 (manufactured by HORIBA), and the solid content concentration was measured using a digital sugar meter APAL-1 (manufactured by AS ONE). The viscosity was measured using a vibration type viscometer VISCOMATE VM-10A (manufactured by CBC).

As a result of Example 4, the polyamine concentration was 0.0082%, and the relative polyamine concentration was 49.4% when the polyamine concentration of Comparative Example 1 was 100. The solid content concentration was 8.6%, the NaCl concentration was 0.00%, the NaCl concentration per solid content was 0.00%, and the viscosity was 4.3 mPa · s.

(Example 5)
(1) 400 g of wheat germ was added to 1600 g of pure water, and the mixture was stirred at 100 rpm for 4 hours using a stirrer (three-one motor, manufactured by HEIDON) under the condition of a water temperature of 40 ° C.
(2) Next, the mixture was centrifuged at 8000 rpm for 20 minutes, and the resulting supernatant was used as Extract C.
(3) The polyamine concentration, NaCl concentration, solid content concentration, and viscosity of Extract C were measured. The NaCl concentration was measured using a compact salinity meter C-121 (manufactured by HORIBA), and the solid content concentration was measured using a digital sugar meter APAL-1 (manufactured by AS ONE). The viscosity was measured using a vibration type viscometer VISCOMATE VM-10A (manufactured by CBC).

As a result of Example 5, the polyamine concentration was 0.0099%, and the relative polyamine concentration was 59.6% when the polyamine concentration of Comparative Example 1 was 100. The solid content concentration was 8.6%, the NaCl concentration was 0.00%, the NaCl concentration per solid content was 0.00%, and the viscosity was 7.5 mPa · s.

(Example 6)
(1) 400 g of wheat germ was added to 1600 g of pure water, and the mixture was stirred at 100 rpm for 4 hours using a stirrer (three-one motor, manufactured by HEIDON) under the condition of a water temperature of 60 ° C.
(2) Next, the mixture was centrifuged at 8000 rpm for 20 minutes, and the resulting supernatant was used as Extract F.
(3) The polyamine concentration, NaCl concentration, solid content concentration, and viscosity of the extract F were measured. The NaCl concentration was measured using a compact salinity meter C-121 (manufactured by HORIBA), and the solid content concentration was measured using a digital sugar meter APAL-1 (manufactured by AS ONE). The viscosity was measured using a vibration type viscometer VISCOMATE VM-10A (manufactured by CBC).

As a result of Example 6, the polyamine concentration was 0.0099%, and the relative polyamine concentration was 59.6% when the polyamine concentration of Comparative Example 1 was 100. The solid content concentration was 8.9%, the NaCl concentration was 0.00%, the NaCl concentration per solid content was 0.00%, and the viscosity was 16.7 mPa · s.

(Comparative Example 2)
(1) 400 g of wheat germ was added to 1600 g of pure water, and the mixture was stirred at 100 rpm for 4 hours using a stirrer (Three-One Motor, manufactured by HEIDON) under the condition of a water temperature of 90 ° C.
(2) Next, the mixture was centrifuged at 8000 rpm for 20 minutes, and the resulting supernatant was used as extract G.
(3) The polyamine concentration, NaCl concentration, solid content concentration, and viscosity of the extract G were measured. The NaCl concentration was measured using a compact salinity meter C-121 (manufactured by HORIBA), and the solid content concentration was measured using a digital sugar meter APAL-1 (manufactured by AS ONE). The viscosity was measured using a vibration type viscometer VISCOMATE VM-10A (manufactured by CBC).

As a result of Comparative Example 2, the polyamine concentration was 0.0103%, and the relative polyamine concentration was 62.0% when the polyamine concentration of Comparative Example 1 was 100. Although the polyamine concentration showed a relatively high value, the viscosity was 1000 mPa · s or more, which was extremely viscous and unsuitable for the production of a polyamine composition. The solid concentration was 11.9%, the NaCl concentration was 0.00%, and the NaCl concentration per solid content was 0.00%.

The polyamine composition of the present invention has a low salt concentration and is useful for blending into foods, cosmetics, pharmaceuticals, quasi drugs, animal feeds and the like. Furthermore, according to the present invention, since a large-scale stainless steel tank used for industrial chemical production can be used, mass production is possible and useful.

Claims (9)

(1) The process of processing a plant and / or a plant processed material with water, and (2) The process of isolate | separating and extract | collecting a liquid fraction, The manufacturing method of the polyamine composition characterized by the above-mentioned. The method for producing a polyamine composition according to claim 1, wherein the step of treating the plant and / or the processed plant product with water is performed at a water temperature of 10 ° C to 60 ° C for 30 minutes to 24 hours. The method for producing a polyamine composition according to claim 2, wherein the step of treating the plant and / or the processed plant product with water is performed at a water temperature of 15 ° C to 45 ° C for 30 minutes to 16 hours. The method for producing a polyamine composition according to claim 3, wherein the step of treating the plant and / or the processed plant product with water is performed at a water temperature of 20 ° C to 30 ° C for 1 hour to 6 hours. Plant and / or plant processed product derived from at least one selected from the group consisting of soybean seed, soybean germ, soybean embryo, wheat seed, wheat germ, wheat embryo, soy milk and okara The manufacturing method of the polyamine composition in any one of Claims 1-4 which is these. The plant and / or processed plant product is a plant and / or processed plant product derived from at least one selected from the group consisting of wheat seed, wheat germ and wheat embryo. A process for producing a polyamine composition. The polyamine composition is 1,3-diaminopropane, putrescine, cadaverine, cardine, spermidine, homospermidine, aminopropyl cadaverine, theremin, spermine, thermospermine, canabalmine, aminopentylnorspermidine, N, N-bis (aminopropyl) The polyamine composition according to claim 1, which is a composition containing at least one compound selected from the group consisting of cadaverine, homospermine, cardopentamine, homocardopentamine, cardohexamine and homocardohexamine. Manufacturing method. The method for producing a polyamine composition according to claim 7, wherein the polyamine composition is a composition comprising at least one compound selected from the group consisting of putrescine, cadaverine, spermidine and spermine. A polyamine composition produced from a plant and / or a processed plant product, wherein the polyamine composition does not substantially contain sodium chloride or sodium citrate in the solid content.