JP2012144510A - Sericin ion water - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide new sericin ion water which can exhibit desired effects of sericin ion water without being influenced by acidic or alkaline characteristics of a target for use such as plants and biological living bodies and without exerting adverse influences on the target for use, and which can be efficiently manufactured.SOLUTION: The sericin ion water is efficiently obtained by charging cocoons, Dead Sea salt, protease (EC3, 4, 24, 27) into pure water, hydrolyzing the mixture for 2 to 24 hours while heating at 50 to 70°C, and separating the resultant aggregates by the use of a filter. The obtained sericin ion water has superior effects in a growth promoting function, antioxidant function, moisturizing function, skin whitening function and the like, and less influences plants and living bodies.

Description

Detailed Description of the Invention

Industrial application fields

  The present invention relates to sericin ion water, and more particularly to a novel sericin ion water that has excellent effects on growth promoting function, antioxidant function, moisturizing function, whitening function, etc. and has little influence on plants and living organisms. .

  As a conventional sericin extraction method, for example, a temperature treatment as disclosed in Japanese Patent No. 3516059, Japanese Patent No. 3716293, Japanese Patent No. 3959452, and the like is known. These sericin extraction methods have certain characteristics, and if the target of use conforms to the characteristics, the desired effect can be obtained, but the target of use may not be expected due to changes in the characteristics, conditions, etc. The application range is limited. Thus, the development of sericin extraction that exhibits a desired effect in a range of use limited to the conventional sericin extraction method and that exhibits a desired sericin extraction effect is desired.

Problems to be solved by the invention

  The present invention has been made in view of the above-mentioned conventional circumstances, and its object is to ionize using salt from the Dead Sea, extract sericin from straw using an enzyme using bacterial endo-type protease, and use thereof An object of the present invention is to provide a novel sericin ion water capable of exhibiting a desired growth promoting function, antioxidant function, moisturizing function, and whitening function without adversely affecting an elephant.

Means for solving the problem

  In order to achieve the above object, the inventor of the present application pays attention to the involvement and action of Dead Sea salt, bacterial endo-type protease (EC3,4,24,27) as important factors in the production of sericin ion water. As a result of intensive research, unlike the conventionally known techniques, the stability of sericin ion water can be improved by introducing salt, dead sea salt, and bacteria-derived endo-type proteases. It was found that sericin concentration of 0.01 to 0.15% sericin ion water obtained by making salt and bacteria-derived endo-type proteases within a predetermined range can be stabilized within the range of PH6 to 8. .

  Furthermore, the Dead Sea salt of the present invention, bacterial endo-type protease (EC3,4,24,27) are useful, and such anther, Dead Sea salt, bacterial-derived endoprotease (EC3,4,24). 27) is put into pure water and extracted at 50 to 70 ° C. for 2 to 24 hours, and then fibroin and other impurities are removed using a filter. The inventors have found that the present invention is extremely useful for solving the above problems and have completed the present invention.

  In other words, the present invention contains pure water containing shark, Dead Sea salt, bacterial endo-type protease (EC3, 4, 24, 27), extracted at 50-70 ° C. for 2-24 hours, and then used fibroin and other filters. Sericin ion water produced by removing impurities, neutral sericin ion water having a sericin concentration of 0.01 to 0.15%, salts 0.001 to 0.01%, and pH 6 to 8. Examples of the method for producing sericin ion water according to the present invention include the following examples. First, raw water (1) pure water treatment (2) predetermined salt, Dead Sea salt, and bacteria-derived endo-type protease (EC 3, 4, 24, 27) are added to the pure water. (3) The obtained aggregate is hydrolyzed for 2 to 24 hours while heating at 50 to 70 ° C. (4) The resulting aggregate is separated from sericin ion water using a filter. (5) Sericin concentration (0. 01 to 0.15%) and containing a trace salt concentration (0.001 to 0.1%) to obtain neutral sericin ion water of PH6 to 8 (6) For example, pharmaceuticals (cosmetics), plants, etc. It is also useful when used as a growth promoter or food additive.

  In addition, the sericin ion water of the present invention takes into consideration the use in a stock solution and the use of this stock solution diluted by about 10 to 1,000 times. That is, sericin ion water containing a sericin salt having a sericin concentration of 0.01 to 0.15% and a salt concentration of 0.001 to 0.1% when used in a stock solution. When diluted, sericin ion water containing a sericin salt having a sericin concentration of 0.001 to 0.000015%, a salt concentration of 0.001 to 0.0001%, and a pH of 6 to 8 is used.

The above salts are preferably at least Dead Sea salt, and the enzyme is a bacterial endo-type protease (EC3,4,24,27).

  The salt of the Dead Sea is obtained by drying the lake water of the Dead Sea existing in nature, and it is most preferable to use a bacterial-derived endo-type protease (EC 3, 4, 24, 27).

  The raw material is salmon, Dead Sea salt, bacterial endo-type protease (EC3,4,24,27), and the original is 5 to 50, 0.001 to 0.1, 0.05 to pure water. It is preferable to contain in the range of 3 weight%.

  By introducing Dead Sea salt into pure water as in the present invention, sericin ion water having a predetermined concentration can be obtained which is stable for a long time. In addition, the obtained sericin ion water is stable between PH 6-8, and is used in a gentle and relaxed state with respect to the use target of plants, living organisms, etc., without affecting the acid or alkali properties of the use target. The effect of can be obtained. Therefore, the growth promoting function, antioxidant function, moisturizing function, and whitening function effect possessed by sericin ion water can be directly expressed, and these effects can be obtained without being influenced by the characteristics of the object to be used. In addition, sericin ion water with a sericin concentration of 0.01 to 0.15% and containing salts, which promotes various effects such as growth promotion function, antioxidant function, moisturizing function, whitening function, etc. with sericin ion water. I can plan. When the sericin concentration is as high as 0.01% or more, remarkable effects are obtained in the antioxidant function, the moisturizing function, the whitening function and the like.

  By ionizing with salmon, Dead Sea salt, and enzymes (bacterial-derived endoprotease), various effects such as growth promoting function, antioxidant function, moisturizing function, whitening function, etc. are enhanced by sericin ion water. An effect is obtained.

  The salt of the Dead Sea uses what was taken from the natural world (the dried water of the Dead Sea Lake), and the enzyme (bacterial endoprotease) uses heat treatment to reduce the impact on the target of use while reducing the effect on the future. I can get it.

Hereinafter, embodiments will be described.
The soot extract of this example is a conventionally known heating method in which soot is added to water and heat is added. Alkaline heating method in which soot is added to alkaline water of PH8 or higher, soot is added to acidic water of PH5 or lower Unlike heat-added acidic heating method or pouring water into water, and adding high heat to high heat method, pouring water, dead sea salt and enzyme (bacterial endoprotease) into pure water. In addition, it is obtained by a hydrolysis method using an enzyme containing salts.

  As a result of comparative tests on the sedimentation rate of the koji extract, the conventionally known heating method in which koji is added to water is 100% at the time of production, 0.085% after one month, and 0 after two months. 074%, decreased by 0.068% after 3 months. 0.01% at the time of sericin ion water production, 0.01% after 1 month, 0.01% after 2 months, 0.01% after 3 months. The conventional manufacturing method has fallen below a predetermined concentration as a result of the sedimentation rate. As for the sericin ion water, it is estimated that the predetermined concentration is in a preferable range as a result of the sedimentation rate. (Table 1)

Characteristics of sericin Sericin is a protein that accounts for 20 to 30% of sputum.
The amino acid composition of sericin is as shown in the table. (Table 2)
At that time, the polypeptide chain has the property of having excellent water retention and water retention and being dispersed in water because side chains of new water groups frequently appear in OH, COOH, NH _{2 and the} like.

It was confirmed by experiments that sericin suppresses the transmission of UV light having a wavelength that is most harmful to the human body and 200 to 300 nanometers. (Table 3)
Moisturizing effect Sericin contains serine, which is an amino acid deeply related to the moisturizing effect. Serine is the most abundant amino acid in natural moisturizing factor (NMF) that is contained in human skin and has a water retention effect. The difference in the amino acid composition of the stratum corneum between normal skin and troubled skin is the content of serine. It is said that this is the difference. In other words, sericin is a protein of the same type as human skin, has good water retention and works to moisturize the skin. (Table 4)
Antioxidant effect (free radical scavenging activity)
The effect of suppressing the action of active oxygen of sericin is at the same level as vitamin C, and is expected to have an effect of preventing the generation of wrinkles and spots. As a result of testing the effect of the soot layer on the free radical scavenging activity, a certain scavenging activity was observed. It has been suggested that free radical scavenging activity is related to the sericin layer. (Table 5) (Table 6)
Tyrosinase activity inhibitory effect (whitening effect)
It was proved that the addition of 100 μg / ml and 200 μg / ml of sericin ion water significantly reduced the tyrosinase activity to 61% and 48% compared to the control.

  From the above test results, it was confirmed that the sericin ion water according to the present invention exerted remarkable effects on the growth promoting function, antioxidant function, moisturizing function, whitening function and the like.

  Based on each of the above tests, it also exhibits growth promoting functions, antioxidant functions, moisturizing functions, whitening functions, etc. in pharmaceuticals (quasi-drugs, cosmetics) foods.

The invention's effect

  As described above, the present invention is a sericin ion water produced by an extraction method using salmon, Dead Sea salt, and protease (EC 3, 4, 24, 27). Is stable and efficient. Moreover, it is made into neutral sericin ion water of PH6-8 by the combination of Dead Sea salt and protease (EC3, 4, 24, 27), and it is familiar with the use object such as plants, organisms and living bodies in a relaxed state. Directly express the characteristics of sericin ion water without affecting the acid or alkali characteristics of these objects of use, growth promotion function, antioxidant function, moisturizing function, whitening function regardless of the characteristics of the object of use A remarkable effect can be obtained.

  When the sericin concentration was set to a high concentration of 0.01 to 15% or more, remarkable effects could be obtained by the growth promoting function, antioxidant function, moisturizing function, whitening function and the like.

  When the salt of the Dead Sea is made of five kinds of salts consisting of magnesium chloride, sodium chloride, calcium chloride, potassium chloride and other trace metals, various effects such as growth promoting function, antioxidant function, moisturizing function, whitening function, etc. by sericin ion water Can be improved and a more remarkable effect can be obtained.

  Protease (EC3,4,24,27) is a hydrolysis reaction catalyst using cocoon as a substrate. Sericin ion water produced using a protease can promote various effects such as a growth promoting function, an antioxidant function, a moisturizing function, and a whitening function, and can obtain a more remarkable effect.

Schematic diagram showing the outline of the sericin ion water production process

Claims (5)

  Cut pupae, remove pupae, remove impurities, wash pure pupae, Dead Sea salt, bacterial endoprotease (EC3,4,24,27) substrate and heat at 50-70 ° C for 2-24 hours to hydrolyze After the reaction, fibroin is removed using a filter. A produced sericin ion water having a sericin concentration of 0.01 to 0.15%, a trace amount of salts, and a pH of 6 to 8.   The sericin ion water according to claim 1, wherein the sericin concentration is 0.01 to 0.15%.   In the sputum (sericin 20-30% fibroin 70-80%) sericin amino acid composition is serine, aspartic acid, glycine, threonine, glutamic acid, arginine, alanine, valine, lysine, histidine, leucine, isoleucine, tryptophan, phenylalanine , Proline, cystine) are known. Dead Sea salt consists of at least magnesium chloride, sodium chloride, calcium chloride, potassium chloride, and other trace metals. The sericin ion water according to claim 1 or 2, wherein the protease (EC3,4,24,27) preparation is a hydrolysis reaction catalyst using a protein as a substrate classified into a metalloprotease, which is an endo-type protease derived from Bacillus stearothermophilus, a kind of high-temperature bacteria. .   The above-mentioned Iro-dori is a hybrid of a cross between a Japanese variety of “Iro” and a Chinese species of “Dori” and has a unique amber color. The salt of the Dead Sea is Israeli Dead Sea lake dry salt, protease ( EC3,4,24,27) is contained using Samase PC10F manufactured by Amano Enzyme. Sericin ion water according to claim 1 or 3.   The above-mentioned squid (Irodori), Dead Sea salt, and protease (EC 3, 4, 24, 27) are used as a base, and the base is used for pure water 100. Protease (EC3,4,24,27) is contained in the range of 0.05 to 3% by weight, heated for 2 to 24 hours (50 to 70 ° C), and then cooled to room temperature.

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