JP2012136462A - Skin condition improving composition and usage of the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a composition for replenishing elastin and collagen in a body and enhancing abilities of synthesizing elastin and collagen by replenishing the elastin and the collagen from the outside of a body, and activating functions of the elastin and the collagen.SOLUTION: A skin condition improving composition is prepared by compounding, in 100 parts by weight of the elastin, 100 to 20,000 parts by weight of the collagen, preferably 1,000 to 10,000 parts by weight, 100 to 3,000 parts by weight of L-ascorbic acid, preferably 100 to 1,000 parts by weight, and 1 to 9 parts by weight of copper salts in terms of copper, preferably 1 to 5 parts by weight. The skin condition improving composition is expected to be used for skin condition improving functional foods, cosmetics and drugs containing the composition.

Description

  The present invention relates to a skin quality improving composition and use thereof, and more particularly to a composition containing elastin and use thereof.

  Collagen is a fibrous protein having strong rigidity like steel, and is abundant in skin dermis, tendons, arteries, cartilage, etc. of mammals, birds, fish and the like. Elastin is a fibrous protein having elasticity that expands and contracts like rubber, and is present together with collagen in connective tissues such as the dermis, ligaments, and arteries of the skin of mammals, birds, fish, and the like. Human skin contains a certain proportion of collagen and elastin. The amount of these proteins decreases with age, which causes skin firmness, loss of moisture and wrinkles.

  As a method for restoring and preventing the skin aging phenomenon, functional foods that supplement elastin and collagen from outside the body and increase the synthesis efficiency of elastin and collagen in the body have been studied. For example, Patent Document 1 includes tea leaves obtained by processing olive leaves and collagen, and if necessary, vitamin C, hyaluronic acid, coenzyme Q10, L-carnitine, chondroitin, acetylglucosamine, elastin and asterisk. A food composition comprising at least one selected from chitin sun is disclosed. Since olive leaves are rich in oleuropein, which has an effect of promoting the synthesis of collagen in the body, the in vivo synthesis efficiency of collagen is improved by ingesting collagen and oleuropein simultaneously.

  Patent Document 2 discloses an oral skin aging preventive / ameliorating agent containing two or more mucopolysaccharides, collagen and / or elastin or a partially decomposed product thereof, and coenzyme Q10 as active ingredients. By taking orally, this improving agent is said to have both safety and improvement effects such as skin wrinkles, sagging and dullness due to skin aging, and safety.

  Patent Document 3 contains methylsulfonylmethane, and further contains at least one selected from the group consisting of mucopolysaccharides, amino sugars, collagen, elastin, amino acids, and ceramides, and has a cosmetic effect. Things are disclosed. By ingesting the food composition, it is said that excellent beauty effects such as a whitening effect, an effect of preventing and reducing wrinkles, and an effect of improving skin firmness and gloss are obtained.

JP-A-2006-191845 (food composition and method for producing the same) JP-A-2006-143671 (oral skin aging preventive / improving agent) JP2005-13124 (food composition having beauty effect)

  The above-described conventional techniques are not sufficiently supplemented and synthesized with elastin and collagen in the body, and are not at a satisfactory level as an effect. Accordingly, an object of the present invention is to provide a composition that enhances replenishment and synthesis ability in the body by replenishing elastin and collagen from outside the body, thereby activating the functions of elastin and collagen.

  As a result of intensive studies on the above problems, the present inventors have found that the above problems can be solved by blending vitamins and copper salts in a certain ratio with elastin and collagen. That is, in the present invention, collagen is 100 to 20,000 parts by weight, preferably 10,000 to 10,000 parts by weight, and L-ascorbic acid is 100 to 3,000 parts by weight, preferably 100 parts by weight of elastin. Provides a skin quality improving composition comprising 100 to 1,000 parts by weight and 1 to 9 parts by weight, preferably 1 to 5 parts by weight, of copper salt in terms of copper. In the present specification, the skin quality improving composition is used in the sense of containing an anti-aging agent for skin.

  The copper salt is preferably at least one member of the group consisting of copper gluconate, copper sulfate, copper chlorophyllin sodium, copper chlorophyll and copper yeast.

  Furthermore, it is preferable to mix 5 to 65 parts by weight, preferably 5 to 35 parts by weight of copper gluconate as copper salts.

  Minerals other than copper include zinc, cobalt, nickel, titanium, potassium, calcium, chromium, selenium, iron, magnesium and manganese.

  The present invention also provides a food for improving skin quality, a cosmetic product for improving skin quality for oral use, and a medicament for improving skin quality, comprising the skin quality improving composition.

  The skin quality-improving composition of the present invention has elastin and collagen absorption and synthesis efficiencies as compared with a conventional mixture containing elastin and collagen by blending elastin, collagen, L-ascorbic acid and mineral at a specific ratio. As a result, improved skin firmness and moisture, improved skin wrinkles, improved and prevented skin dullness, improved and prevented rough skin, improved skin color, improved skin texture, etc. Play. Therefore, the skin quality improving composition of the present invention is expected to be used as a functional food or cosmetic containing the composition.

The CCD photograph of the 3D replica of the wrinkles of the eye corner of subject A (50 years old, female) who took the skin quality improving composition (Example 1) according to the present invention is shown. 1: Before ingestion (0 degree analysis) 2: Before ingestion (180 degree analysis) 3: After 8 weeks ingestion (0 degree analysis) 4: After 8 weeks ingestion (180 degree analysis) Here, the 0 degree analysis is Analyzing along the direction of the wrinkles means 180-degree analysis means analyzing from a direction rotated 180 degrees along the wrinkles. In each photograph, the length of the portion where the brightness is increased is detected as the width of the wrinkle and the length of the portion which is shaded as the depth of the wrinkle. A CCD photograph of a 3D replica of a wrinkle at the corner of an eye of a subject B (52 years old, female) who took collagen alone is shown. 1: Before ingestion (0 degree analysis) 2: Before ingestion (180 degree analysis) 3: After 8 weeks ingestion (0 degree analysis) 4: After 8 weeks ingestion (180 degree analysis) A CCD photograph of a 3D replica of the texture of cheek of subject A is shown. 1: Before ingestion (0 degree analysis) 2: Before ingestion (180 degree analysis) 3: After 8 weeks ingestion (0 degree analysis) 4: After 8 weeks ingestion (180 degree analysis) In each photo, the brightness increases The length of the existing part is detected as the width of the texture and the length of the shadowed part as the depth of the texture. A CCD photograph of a 3D replica of the texture of cheek of subject B is shown. 1: Before ingestion (0 degree analysis) 2: Before ingestion (180 degree analysis) 3: After 8 weeks ingestion (0 degree analysis) 4: After 8 weeks ingestion (180 degree analysis)

  Elastin is a major protein present together with collagen in mammals, birds, fish, etc., particularly in mammals' aorta, ligament ligament, yellow ligament, lung, skin, uterus, elastic cartilage and the like. As physical functions of elastin, elasticity and durability which are mechanical properties are well known. The loss of elastic function of elastin is associated with quantitative, qualitative and structural changes associated with pathological aging such as aging and arteriosclerosis. Elastin also has biological functions such as cell migration and cell proliferation. It is presumed that physical and biological functions such as quantitative, qualitative and structural improvement, elastic function and the like of elastin are involved in the skin quality improving function in the present invention.

  Elastin exists as an insoluble protein having a three-dimensional network structure in vivo. Elastin can be extracted from animal biological tissues, for example, connective tissues such as mammals, birds, fish, etc., by hydrolyzing elastin with an acid or alkali, or by enzymatic treatment. The elastin used in the present invention includes hydrolyzed elastin, α-elastin, κ-elastin and elastin peptide in addition to water-soluble elastin.

  Water-soluble elastin is insoluble obtained from animal living tissue as described in Patent 4078431 (water-soluble elastin and its production method and food and medicine containing the same), which is part of the inventor. Elastin purified from an elastin-soluble solubilized solution obtained by dissolving elastin in a certain alkaline solution, and a low molecular weight having a molecular weight of about 50,000 or less in which the content of amino acids constituting elastin is specified It is water-soluble elastin. Preferably, it is a low molecular weight water-soluble elastin having a molecular weight of about 30,000 or less.

  The method for producing water-soluble elastin includes (1) a step of obtaining insoluble elastin by removing collagen and other unnecessary proteins from animal living tissue, and (2) dissolution of insoluble elastin in a solubilized solution. A step of obtaining a solubilized solution, and (3) a step of recovering low molecular weight water-soluble elastin from the elastin-dissolved solubilized solution.

  Examples of animal living tissues include the ligamentum ligament, aortic blood vessels and the like obtained from mammals such as pigs, horses, cows, and sheep. In addition, arterial spheres obtained from fish such as skipjack, tuna, yellowtail and salmon are also included. Animal biological tissue can be easily homogenized with a mixer, meat chopper, or the like to easily remove unnecessary proteins such as collagen. The homogenized animal living tissue is appropriately degreased with hot water, a hot dilute alkaline aqueous solution or an organic solvent.

  The treatment for removing the collagen and other unnecessary proteins is performed by immersing the animal living tissue in an alkaline solution of 0.01 to 0.5 M, preferably 0.05 to 0.5 M, at 90 to 105 ° C. for 10 to 20 minutes. And do it. Prior to the treatment for removing collagen and other unwanted proteins, it is preferable to immerse the animal living tissue in a 0.1 to 2 M salt solution at room temperature for 1 hour.

  The solubilized solution is obtained by heating an acidic solution of 0.1 to 0.5M or an alkaline aqueous solution of 0.01 to 1.0M, preferably 0.05 to 0.5M, to a liquid temperature of 90 to 105 ° C. .

  The water-soluble elastin obtained by the above production method comprises 78-85% of the amino acids constituting elastin consisting of proline, glycine, alanine and valine, 2-4% consisting of aspartic acid and glutamic acid, and 1-2% lysine. Histidine and arginine, and 0.2-0.4% consists of desmosine and isodesmosine.

  The origin of collagen, which is an essential component of the skin quality improving composition of the present invention, is not particularly limited. For example, it is extracted and purified from pigs, fish, birds and the like based on a known method. Collagen used in the present invention includes hydrolyzed collagen, water-soluble collagen and collagen peptide. The collagen peptide is obtained, for example, by washing the dermis of an animal, neutralizing it after alkali treatment, obtaining an extract containing collagen, and then decomposing the extract with a collagenase enzyme. The peptide is appropriately filtered, sterilized, dried and then powdered. As the collagen, hydrolyzed collagen, water-soluble collagen and collagen peptide, commercially available products can be used.

  Human skin contains approximately 50 to 1 collagen and elastin. This ratio is important for maintaining normal skin function. Therefore, also in the skin quality improving composition of the present invention, elastin and collagen are contained in the above ratio. Specifically, in the skin quality improving composition of the present invention, collagen is 100 to 20,000 parts by weight, preferably 1,000 to 10,000 parts by weight, more preferably 3 parts per 100 parts by weight of elastin. 1,000 to 7,000 parts by weight.

  L-ascorbic acid is essential for the skin quality improving composition of the present invention. By using L-ascorbic acid as an essential component, synthesis of collagen in the body is promoted.

  The content of L-ascorbic acid in the skin quality improving composition of the present invention is 100 to 3,000 parts by weight, preferably 100 to 1,000 parts by weight with respect to 100 parts by weight of elastin.

  Copper salts are essential for the skin quality improving composition of the present invention. This promotes the synthesis of elastin in the body. Specific examples of the copper salts include copper gluconate, copper sulfate, copper yeast, copper chlorophyllin sodium, copper chlorophyll and the like, and preferably copper gluconate.

  Content of the copper salt in the skin quality improvement composition of this invention is 1-9 weight part in conversion of copper with respect to 100 weight part of elastin, Preferably it is 1-5 weight part. For example, copper gluconate is 5 to 65 parts by weight, preferably 5 to 35 parts by weight, based on 100 parts by weight of elastin.

  As long as the object of the present invention is not impaired, the skin quality improving composition of the present invention, besides the skin quality improving composition of the present invention, minerals other than copper, proteins, sugars, fats, trace elements, vitamins, sweeteners , Fragrances, colorants and the like can be added as appropriate.

  Minerals other than copper include zinc, cobalt, nickel, titanium, potassium, calcium, chromium, selenium, iron, magnesium and manganese.

  The form of the skin quality improving composition of the present invention is not particularly limited, such as pills, tablets, capsules, granules, granules, powders, syrups, drops, liquids, biscuits, jellies, gels and the like. In order to increase the intake of elastin, pills, tablets, liquids, powders, granules and the like are preferable.

  The skin quality improving composition of the present invention can be expected to have an effect of compensating for a decrease in elastin and collagen synthesis efficiency due to aging. The skin quality improving composition of the present invention improves skin firmness and moisture, improves skin wrinkles, improves and prevents skin dullness, improves and prevents rough skin, improves skin color, and improves skin texture. Served as functional food for quality improvement.

  The skin quality improving composition of the present invention, when orally ingested, exhibits functions desired by cosmetics such as prevention of skin firmness and texture improvement wrinkles. These functions are also functions of cosmetics for the purpose of beauty, and therefore the skin quality improving composition of the present invention can be said to be a cosmetic by oral intake. Therefore, the present invention also provides an oral skin quality improving cosmetic containing a skin quality improving composition.

  The skin quality improving composition of the present invention is also effective in healing and preventing diseases associated with skin abnormalities such as atopic dermatitis, housewife eczema, and hand eczema.

  The dose of the skin quality improving composition of the present invention is usually an amount corresponding to 10 to 500 mg, preferably 50 to 200 mg, more preferably 75 to 125 mg in terms of elastin per day for an adult.

  Hereinafter, the present invention will be described in more detail by showing examples of the present invention. However, the present invention is not limited to the following examples.

[Example 1]
1. Preparation of skin quality improving composition Low molecular weight water-soluble elastin was prepared from connective tissue of pigs according to the method described in Japanese Patent No. 4078431. Specifically, after removing a portion of the porcine aorta having a low elastin content such as fat and muscle with a blade, it was homogenized with a homogenizer. The homogenized tissue was boiled in boiling water having a volume twice that of the tissue for 10 minutes, and this operation was repeated to degrease. The defatted tissue is immersed in a 1M sodium chloride aqueous solution having a volume twice that of the tissue and stirred at room temperature for 50 minutes, and further immersed in a 1M sodium chloride aqueous solution having a volume 10 times that of the tissue and stirred at room temperature for 1 hour to remove unwanted proteins. Removed. The soaked tissue was placed in a 7.5-fold volume of 0.1 M aqueous sodium hydroxide solution and stirred at 100 ° C. for 15 minutes to remove collagen. This operation was repeated. Next, the tissue and the alkaline solution were separated to obtain highly insoluble elastin. Next, 10 volumes of 0.2 M sodium hydroxide solubilization solution was added to insoluble elastin, and the insoluble elastin was fragmented by stirring at 100 ° C. for 2 hours. Thereafter, the solution was neutralized, cooled to 25 ° C. or lower, and filtered through 200 mesh filter paper. Next, desalting is performed for 4 hours or more using an NF membrane (MW 1,000 to 2,000) at 10 to 45 ° C., and sterilization is performed at 95 ° C. for 30 minutes. It was recovered as a powder.

  The elastin obtained above contains collagen (product name: C-peptide, produced by Zerais Co., Ltd.), L-ascorbic acid (product name: L-ascorbic acid (vitamin C), produced by BASF Japan Ltd.), and gluconic acid. Copper (product name: food additive copper gluconate, manufactured by Tomita Pharmaceutical Co., Ltd.) was blended in the ratio shown in Table 1. The preparation was packaged in a bag so as to be 100 mg in terms of elastin. As a positive control, collagen well known as a moisturizing and anti-aging material was packaged in the amount shown in Table 1.

2. Evaluation of skin quality improving composition In order to verify the skin improvement effect by ingestion of the skin quality improving composition of the present invention, the skin quality improving composition (Example 1) and collagen (Comparative Example 1) shown in Table 1 were used. A single-blind parallel group comparison test was conducted (* 2 group comparison). The results were statistically processed by a t-test with before / after comparison / correspondence and a t-test without comparison / intergroup comparison, and the significance level was set to 5% or less by a two-sided test.

The total number of subjects was 8 for the examples and 8 for the comparative examples. Subjects must have the following conditions:
(1) Japanese women aged 40 to 60 years (2) Those who are worried about wrinkles in the corners of the eyes (3) Those who are worried about makeup flare, sagging skin, and dry skin (4) During the test period Those who can refrain from direct sunlight (sunburn) and those who can prevent ultraviolet rays (5) Existing functional foods for the purpose of improving skin quality during the test period (for example, elastin, collagen, ceramide, hyaluronic acid fortification) (6) Persons who are satisfied with all who can prohibit the change or addition of cosmetics during the test period.

  Each subject took the test product of Example 1 or Comparative Example 1 that was made indistinguishable in appearance and mixed with 160 mL of orange juice once a day before going to bed. The intake period was 8 weeks.

  A 3D replica analysis of the skin was performed on subjects before taking the test product and 8 weeks after taking the test product. This 3D replica analysis is a technique for determining the effects of long-term use of cosmetics, skin care supplements, and the like by collecting replicas over time from the cheeks and the corners of the eyes and analyzing the texture and wrinkle state. Specifically, a liquid silicone was applied to the sampling site of the test subject to solidify, and the solidified silicone was peeled off to prepare a texture / wrinkle replica of the sampling site. In the measurement of the texture, a 3D replica was collected from the center value of the cheek connecting the subject's left armpit and the lip. In the measurement of wrinkles, 3D replicas were collected from the left eye corner wrinkles of the subject.

  Light was applied to these 3D replicas from an angle, and the bright and shadowed parts were photographed with a CCD camera. 1 to 4 show CCD images of replica images.

  Next, using a commercially available 3D replica analysis system for reflection (product name: ASA-03RXD, manufactured by Asahi Biomed), after binarizing the image, the length of the portion where the brightness is increased is the width of the texture / wrinkle And the length of the shadowed part was detected as the depth of the texture and wrinkles.

The evaluation items for texture measurement are the following two types.
Texture volume ratio: A sum of those determined to be texture in the designated area as an average value per 1 mm ^{2 and} 1/100. An increase in this value before and after intake indicates an improvement in skin.
Number of textures: The average value within the designated area of the number of textures determined to be texture in 1 mm of analysis in the horizontal direction with respect to the light source. An increase in this value before and after intake indicates an improvement in skin.

The evaluation items of wrinkle measurement are the following four types.
Wrinkle volume ratio: The sum of those determined to be wrinkles in the designated area is expressed as an average value per 1 mm ^{2 and} 1/100. A decrease in this value before and after intake indicates an improvement in skin.
Maximum wrinkle depth: Calculated from the length of the shadow in the horizontal direction with the light source on the replica. A decrease in this value before and after intake indicates an improvement in skin.
Number of wrinkles: The average value within the designated area of the number of wrinkles that were judged to be fine in 1 mm of analysis in the horizontal direction with respect to the light source. A decrease in this value before and after intake indicates an improvement in skin.

  From Table 2, in Example 1, the skin improvement effect was recognized from the result of the texture volume ratio and the number of textures. In particular, the texture volume ratio was not improved in the skin condition in Comparative Example 1, whereas a clear improvement was observed in Example 1. There were also statistically significant differences between the groups.

  Since this test was performed at a time when the humidity of the outside air decreased and the texture and firmness of the skin deteriorated, no clear improvement was observed in Comparative Example 1. On the other hand, in Example 1, an improvement tendency of about 20% in the wrinkle volume ratio and about 10% in the number of wrinkles was observed.

The following questionnaire was conducted on the subjects.
Survey method: Skin elasticity and elasticity, skin shine, skin moisture, skin roughness, makeup glue, pore conspicuousness, wrinkles and fine wrinkles were answered on a 100 mm line segment.
Observation period: Before ingestion and 8 weeks after ingestion Analytical methods: Comparison before and after / corresponding t-test, comparison between groups / t-test without correspondence

  From Table 3, it is thought that the improvement effect with respect to the skin was able to be caught as a bodily sensation in both the Example and the Comparative Example.

  As described above, it was confirmed that the skin quality improving composition of the present invention is more effective than collagen alone. That is, the composition of the present invention has an effect of improving the skin condition by the interaction of components, and is particularly excellent in the effect of improving skin texture and wrinkles.

Claims (5)

  100 to 20,000 parts by weight of collagen, 1,000 to 10,000 parts by weight of collagen, 100 to 3,000 parts by weight of L-ascorbic acid, parts by weight and copper salts in terms of copper with respect to 100 parts by weight of elastin A skin quality improving composition comprising 1 to 9 parts by weight.   The skin quality improving composition according to claim 1, wherein the copper salt is at least one member of the group consisting of copper gluconate, copper sulfate, copper chlorophyllin sodium, copper chlorophyll and copper yeast.   The skin quality improving composition according to claim 1, wherein 5 to 65 parts by weight of copper gluconate is blended as the copper salts.   The food for skin quality improvement containing the skin quality improvement composition in any one of Claims 1-3.   An oral skin quality improving cosmetic comprising the skin quality improving composition according to any one of claims 1 to 3.